Jan E Brinchmann
Affiliation: University of Oslo
- Expanding autologous multipotent mesenchymal bone marrow stromal cellsJan E Brinchmann
Cell Therapy, Institute of Immunology, Rikshospitalet Medical Centre, Oslo, Norway
J Neurol Sci 265:127-30. 2008..To obtain the best cell culture conditions, a number of methodological decisions have to be made. Special considerations are necessary if the cells are to be used for the treatment of patients...
- In vitro expansion of human mesenchymal stem cells: choice of serum is a determinant of cell proliferation, differentiation, gene expression, and transcriptome stabilityAboulghassem Shahdadfar
Institute of Immunology, Rikshospitalet University Hospital, 0027 Oslo, Norway
Stem Cells 23:1357-66. 2005..Thus, hMSCs may be expanded rapidly and with stable gene expression in AS in the absence of growth factors, whereas FBS induces a more differentiated and less stable transcriptional profile...
- Genetic and epigenetic instability of human bone marrow mesenchymal stem cells expanded in autologous serum or fetal bovine serumJohn Arne Dahl
Department of Biochemistry, Institute of Basic Medical Sciences, Faculty of Medicine, University of Oslo, and Rikshospitalet Medical Centre, Oslo, Norway
Int J Dev Biol 52:1033-42. 2008....
- Phenotype and gene expression of human mesenchymal stem cells in alginate scaffoldsShivali Duggal
Institute of Immunology, Rikshospitalet University Hospital, Oslo, Norway
Tissue Eng Part A 15:1763-73. 2009..As alginate may be entirely dissolved, leaving the cells as single cell suspensions for various analyses, this represents a useful model for the study of cells in 3D cultures...
- Modulation of DNA glycosylase activities in mesenchymal stem cellsGunn A Hildrestrand
Centre for Molecular Biology and Neuroscience and Institute of Medical Microbiology, Rikshospitalet University Hospital, Oslo, Norway
Exp Cell Res 315:2558-67. 2009..The glycosylase activities remained stable through at least 12 passages, suggesting that DNA repair is proficient through the period required for in vitro expansion of AT-MSCs to clinically relevant numbers...