Thomas Arnesen

Summary

Affiliation: University of Bergen
Country: Norway

Publications

  1. pmc Identification and characterization of the human ARD1-NATH protein acetyltransferase complex
    Thomas Arnesen
    Department of Molecular Biology, University of Bergen, N 5020 Bergen, Norway
    Biochem J 386:433-43. 2005
  2. pmc Protein N-terminal acetylation: NAT 2007-2008 Symposia
    Thomas Arnesen
    Department of Molecular Biology, University of Bergen, N 5020 Bergen, Norway
    BMC Proc 3:S1. 2009
  3. pmc N-terminal acetylation by NatC is not a general determinant for substrate subcellular localization in Saccharomyces cerevisiae
    Henriette Aksnes
    Department of Molecular Biology, University of Bergen, Bergen, Norway
    PLoS ONE 8:e61012. 2013
  4. pmc The human N-alpha-acetyltransferase 40 (hNaa40p/hNatD) is conserved from yeast and N-terminally acetylates histones H2A and H4
    Kristine Hole
    Department of Molecular Biology, University of Bergen, Bergen, Norway
    PLoS ONE 6:e24713. 2011
  5. pmc NatF contributes to an evolutionary shift in protein N-terminal acetylation and is important for normal chromosome segregation
    Petra Van Damme
    Department of Medical Protein Research, Ghent University, Ghent, Belgium
    PLoS Genet 7:e1002169. 2011
  6. pmc A synopsis of eukaryotic Nalpha-terminal acetyltransferases: nomenclature, subunits and substrates
    Bogdan Polevoda
    Department of Biochemistry and Biophysics, University of Rochester Medical Center, Rochester, NY 14642, USA
    BMC Proc 3:S2. 2009
  7. pmc The chaperone-like protein HYPK acts together with NatA in cotranslational N-terminal acetylation and prevention of Huntingtin aggregation
    Thomas Arnesen
    Department of Molecular Biology, University of Bergen, Thormøhlensgate 55, N 5020 Bergen, Norway
    Mol Cell Biol 30:1898-909. 2010
  8. pmc Towards a functional understanding of protein N-terminal acetylation
    Thomas Arnesen
    Department of Molecular Biology, University of Bergen, Bergen, Norway
    PLoS Biol 9:e1001074. 2011
  9. pmc A novel human NatA Nalpha-terminal acetyltransferase complex: hNaa16p-hNaa10p (hNat2-hArd1)
    Thomas Arnesen
    Department of Molecular Biology, University of Bergen, N 5020 Bergen, Norway
    BMC Biochem 10:15. 2009
  10. ncbi request reprint The protein acetyltransferase ARD1: a novel cancer drug target?
    Thomas Arnesen
    Department of Molecular Biology, University of Bergen, N 5020 Bergen, Norway
    Curr Cancer Drug Targets 8:545-53. 2008

Collaborators

Detail Information

Publications36

  1. pmc Identification and characterization of the human ARD1-NATH protein acetyltransferase complex
    Thomas Arnesen
    Department of Molecular Biology, University of Bergen, N 5020 Bergen, Norway
    Biochem J 386:433-43. 2005
    ..This study identifies the human homologues of the yeast Ard1p and Nat1p proteins and presents new aspects of the NATH and hARD1 proteins relative to their yeast homologues...
  2. pmc Protein N-terminal acetylation: NAT 2007-2008 Symposia
    Thomas Arnesen
    Department of Molecular Biology, University of Bergen, N 5020 Bergen, Norway
    BMC Proc 3:S1. 2009
    ....
  3. pmc N-terminal acetylation by NatC is not a general determinant for substrate subcellular localization in Saccharomyces cerevisiae
    Henriette Aksnes
    Department of Molecular Biology, University of Bergen, Bergen, Norway
    PLoS ONE 8:e61012. 2013
    ..Furthermore, all organelle-localized substrates indicated undisrupted structures, thus suggesting that absence of NatC acetylation does not have a vast effect on organelle morphology in yeast...
  4. pmc The human N-alpha-acetyltransferase 40 (hNaa40p/hNatD) is conserved from yeast and N-terminally acetylates histones H2A and H4
    Kristine Hole
    Department of Molecular Biology, University of Bergen, Bergen, Norway
    PLoS ONE 6:e24713. 2011
    ..Combined, our results strongly suggest that human Naa40p/NatD is conserved from yeast. Thus, the NATs of all classes of N-terminally acetylated proteins in humans now appear to be accounted for...
  5. pmc NatF contributes to an evolutionary shift in protein N-terminal acetylation and is important for normal chromosome segregation
    Petra Van Damme
    Department of Medical Protein Research, Ghent University, Ghent, Belgium
    PLoS Genet 7:e1002169. 2011
    ..With the characterization of NatF, the co-translational N-Ac machinery appears complete since all the major substrate groups in eukaryotes are accounted for...
  6. pmc A synopsis of eukaryotic Nalpha-terminal acetyltransferases: nomenclature, subunits and substrates
    Bogdan Polevoda
    Department of Biochemistry and Biophysics, University of Rochester Medical Center, Rochester, NY 14642, USA
    BMC Proc 3:S2. 2009
    ..ABSTRACT : We have introduced a consistent nomenclature for the various subunits of the NatA-NatE N-terminal acetyltransferases from yeast, humans and other eukaryotes...
  7. pmc The chaperone-like protein HYPK acts together with NatA in cotranslational N-terminal acetylation and prevention of Huntingtin aggregation
    Thomas Arnesen
    Department of Molecular Biology, University of Bergen, Thormøhlensgate 55, N 5020 Bergen, Norway
    Mol Cell Biol 30:1898-909. 2010
    ..Taken together, the data indicate that the physical interaction between HYPK and NatA seems to be of functional importance both for Htt aggregation and for N-terminal acetylation...
  8. pmc Towards a functional understanding of protein N-terminal acetylation
    Thomas Arnesen
    Department of Molecular Biology, University of Bergen, Bergen, Norway
    PLoS Biol 9:e1001074. 2011
    ..These contributions represent new and intriguing hypotheses that will guide the research in the years to come...
  9. pmc A novel human NatA Nalpha-terminal acetyltransferase complex: hNaa16p-hNaa10p (hNat2-hArd1)
    Thomas Arnesen
    Department of Molecular Biology, University of Bergen, N 5020 Bergen, Norway
    BMC Biochem 10:15. 2009
    ..In humans, the homologues hNaa15p (hNat1) and hNaa10p (hArd1) were demonstrated to form a stable ribosome associated NAT complex acetylating NatA type N-termini in vitro and in vivo...
  10. ncbi request reprint The protein acetyltransferase ARD1: a novel cancer drug target?
    Thomas Arnesen
    Department of Molecular Biology, University of Bergen, N 5020 Bergen, Norway
    Curr Cancer Drug Targets 8:545-53. 2008
    ..This review focuses on the enzymatic and biological activities of hARD1, and potential mechanisms of functional inhibition...
  11. pmc Characterization of hARD2, a processed hARD1 gene duplicate, encoding a human protein N-alpha-acetyltransferase
    Thomas Arnesen
    Department of Molecular Biology, University of Bergen, N 5020 Bergen, Norway
    BMC Biochem 7:13. 2006
    ..We recently described the human protein acetyltransferase hARD1 (human Arrest Defective 1). hARD1 interacts with NATH (N-Acetyl Transferase Human) forming a complex expressing protein N-terminal alpha-acetylation activity...
  12. ncbi request reprint Induction of apoptosis in human cells by RNAi-mediated knockdown of hARD1 and NATH, components of the protein N-alpha-acetyltransferase complex
    T Arnesen
    Department of Molecular Biology, University of Bergen, Norway
    Oncogene 25:4350-60. 2006
    ..Our results argue for an essential role of the NATH-hARD1 complex in cell survival and underscore the importance of protein N-alpha-acetylation in mammalian cells...
  13. ncbi request reprint Cloning and characterization of hNAT5/hSAN: an evolutionarily conserved component of the NatA protein N-alpha-acetyltransferase complex
    Thomas Arnesen
    Department of Molecular Biology, University of Bergen, N 5020 Bergen, Norway
    Gene 371:291-5. 2006
    ..In summary, we present the first description of the human homologue of Nat5p/San, hNAT5, the third component of the human NatA N-alpha-acetyltransferase complex...
  14. pmc Proteomics analyses reveal the evolutionary conservation and divergence of N-terminal acetyltransferases from yeast and humans
    Thomas Arnesen
    Department of Molecular Biology, University of Bergen, Bergen, Norway
    Proc Natl Acad Sci U S A 106:8157-62. 2009
    ..Thus, although we revealed different N-acetylation patterns in yeast and humans, the major NAT, NatA, acetylates the same substrates in both species...
  15. pmc Knockdown of human N alpha-terminal acetyltransferase complex C leads to p53-dependent apoptosis and aberrant human Arl8b localization
    Kristian K Starheim
    Department of Molecular Biology, University of Bergen, Thormøhlensgate 55, N 5020 Bergen, Norway
    Mol Cell Biol 29:3569-81. 2009
    ..Taken together, hNatC-mediated N-terminal acetylation is important for maintenance of protein function and cell viability in human cells...
  16. pmc Protein N-terminal acetyltransferases act as N-terminal propionyltransferases in vitro and in vivo
    Håvard Foyn
    Department of Molecular Biology, University of Bergen, N 5020 Bergen, Norway
    Mol Cell Proteomics 12:42-54. 2013
    ....
  17. doi request reprint Identification of the human N(alpha)-acetyltransferase complex B (hNatB): a complex important for cell-cycle progression
    Kristian K Starheim
    Department of Molecular Biology, University of Bergen, N 5020 Bergen, Norway
    Biochem J 415:325-31. 2008
    ..In summary, we show for the first time a vertebrate NatB protein N(alpha)-acetyltransferase complex essential for normal cell proliferation...
  18. pmc N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB
    Petra Van Damme
    Department of Medical Protein Research, Vlaams Instituut voor Biotechnologie, Ghent University, B 9000 Ghent, Belgium
    Proc Natl Acad Sci U S A 109:12449-54. 2012
    ....
  19. pmc Proteome-derived peptide libraries allow detailed analysis of the substrate specificities of N(alpha)-acetyltransferases and point to hNaa10p as the post-translational actin N(alpha)-acetyltransferase
    Petra Van Damme
    Department of Medical Protein Research, VIB, B 9000 Ghent, Belgium
    Mol Cell Proteomics 10:M110.004580. 2011
    ..Thus, complex formation of NatA might alter the substrate specificity profile as compared with its isolated catalytic subunits, and, furthermore, NatA or hNaa10p may function as a post-translational actin N(α)-acetyltransferase...
  20. ncbi request reprint Interaction between HIF-1 alpha (ODD) and hARD1 does not induce acetylation and destabilization of HIF-1 alpha
    Thomas Arnesen
    Department of Molecular Biology, University of Bergen, N 5020 Bergen, Norway
    FEBS Lett 579:6428-32. 2005
    ..Moreover, hARD1 does not acetylate and destabilize HIF-1 alpha. However, we find that hARD1 specifically binds HIF-1 alpha, suggesting a putative, still unclear, connection between these proteins...
  21. pmc Human Naa50p (Nat5/San) displays both protein N alpha- and N epsilon-acetyltransferase activity
    Rune Evjenth
    Department of Molecular Biology, University of Bergen, N 5020 Bergen, Norway
    J Biol Chem 284:31122-9. 2009
    ..In addition, histone 4 was detected as a hNaa50p KAT substrate in vitro. Our findings thus provide the first experimental evidence of an enzyme having both KAT and NAT activities...
  22. doi request reprint Depletion of the human Nα-terminal acetyltransferase A induces p53-dependent apoptosis and p53-independent growth inhibition
    Darina Gromyko
    Department of Molecular Biology, University of Bergen, Bergen, Norway
    Int J Cancer 127:2777-89. 2010
    ..Our findings indicate that several mechanisms of growth inhibition and apoptosis may be induced by hNatA knockdown and that hNatA knockdown could be exploited for use in combinatorial chemotherapy...
  23. ncbi request reprint Bioinformatics analysis of a Saccharomyces cerevisiae N-terminal proteome provides evidence of alternative translation initiation and post-translational N-terminal acetylation
    Kenny Helsens
    Department of Medical Protein Research, VIB, B 9000 Ghent, Belgium
    J Proteome Res 10:3578-89. 2011
    ..Finally, we also observed an unexpected high number of N(α)-acetylated peptides that could not be related to TIS and therefore suggest events of post-translational N(α)-acetylation...
  24. pmc Specificity and versatility of substrate binding sites in four catalytic domains of human N-terminal acetyltransferases
    Cedric Grauffel
    Department of Molecular Biology, University of Bergen, Bergen, Norway
    PLoS ONE 7:e52642. 2012
    ..Finally, by comparing the four structures we propose maps of the peptide-enzyme interactions that should help rationalizing substrate-specificity and lay the ground for inhibitor design...
  25. pmc Human protein N-terminal acetyltransferase hNaa50p (hNAT5/hSAN) follows ordered sequential catalytic mechanism: combined kinetic and NMR study
    Rune H Evjenth
    Department of Molecular Biology, University of Bergen, N 5020 Bergen, Norway
    J Biol Chem 287:10081-8. 2012
    ..This model is further strengthened by the NMR results using a catalytically inactive hNaa50p mutant...
  26. doi request reprint Design, synthesis, and kinetic characterization of protein N-terminal acetyltransferase inhibitors
    Håvard Foyn
    Department of Molecular Biology, University of Bergen, N 5020 Bergen, Norway
    ACS Chem Biol 8:1121-7. 2013
    ..Our demonstration that it is possible to develop NAT selective inhibitors should assist future efforts to develop NAT inhibitors with more drug-like properties that can be used to chemically interrogate in vivo NAT function. ..
  27. doi request reprint HPLC-based quantification of in vitro N-terminal acetylation
    Rune H Evjenth
    Department of Molecular Biology, University of Bergen, Bergen, Norway
    Methods Mol Biol 981:95-102. 2013
    ..We here describe an in vitro method based on reverse-phase HPLC to quantitatively measure in vitro acetylation of NAT oligopeptide substrates, enabling the determination of NAT specificity as well as kinetic parameters...
  28. doi request reprint Protein alpha-N-acetylation studied by N-terminomics
    Petra Van Damme
    Department of Medical Protein Research, VIB, Ghent, Belgium
    FEBS J 278:3822-34. 2011
    ..In this review, we report on such emerging technologies as well as on breakthroughs in our understanding of protein N-terminal biology...
  29. doi request reprint In-gel N-acetylation for the quantification of the degree of protein in vivo N-terminal acetylation
    Petra Van Damme
    Department of Medical Protein Research, VIB, Ghent, Belgium
    Methods Mol Biol 981:115-26. 2013
    ....
  30. doi request reprint Protein N-terminal acetyltransferases: when the start matters
    Kristian K Starheim
    Department of Molecular Biology, University of Bergen, Bergen, Norway
    Trends Biochem Sci 37:152-61. 2012
    ..Here, we discuss how these recent findings shed light on NATs as major protein regulators and key cellular players...
  31. pmc HIV-1 Rev oligomerization is not obligatory in the presence of an extra basic domain
    Clemens Furnes
    Department of Molecular Biology, University of Bergen, N 5020 Bergen, Norway
    Retrovirology 2:39. 2005
    ..However, the biological significance of the obligatory complex formation of Rev upon the viral RNA is unclear...
  32. pmc Composition and biological significance of the human Nalpha-terminal acetyltransferases
    Kristian K Starheim
    Department of Molecular Biology, University of Bergen, N 5020 Bergen, Norway
    BMC Proc 3:S3. 2009
    ..We here summarize the identified N-terminal acetyltransferase complexes in humans, and we review the biological studies on Nalpha-terminal acetylation in humans and other higher eukaryotes...
  33. doi request reprint Outcome after surgery for primary hyperaldosteronism may depend on KCNJ5 tumor mutation status: a population-based study from Western Norway
    Thomas Arnesen
    Department of Surgery, Haukeland University Hospital, 5021 Bergen, Norway
    Langenbecks Arch Surg 398:869-74. 2013
    ....
  34. pmc The protein Nalpha-terminal acetyltransferase hNaa10p (hArd1) is phosphorylated in HEK293 cells
    Hiwa Målen
    Department of Molecular Biology, University of Bergen, N 5020 Bergen, Norway
    BMC Res Notes 2:32. 2009
    ..In the flexible C-terminal tail of hNaa10p, there are several potential phosphorylation sites that might serve as points of regulation...
  35. ncbi request reprint Expression of N-acetyl transferase human and human Arrest defective 1 proteins in thyroid neoplasms
    Thomas Arnesen
    Department of Surgical Sciences, University of Bergen and Haukeland University Hospital, Norway
    Thyroid 15:1131-6. 2005
    ..SiRNA-mediated knockdown of NATH resulted in decreased levels of hARD1 protein. Taken together, these results suggest that NATH positively affects the level of hARD1 protein both in vivo and in cell cultures...
  36. ncbi request reprint A novel type of deletion in the CDKN2A gene identified in a melanoma-prone family
    Stian Knappskog
    Department of Medicine, Section of Oncology, Haukeland University Hospital, N 5021 Bergen, Norway
    Genes Chromosomes Cancer 45:1155-63. 2006
    ....