Thomas Arnesen

Summary

Affiliation: University of Bergen
Country: Norway

Publications

  1. pmc Identification and characterization of the human ARD1-NATH protein acetyltransferase complex
    Thomas Arnesen
    Department of Molecular Biology, University of Bergen, N 5020 Bergen, Norway
    Biochem J 386:433-43. 2005
  2. pmc Protein N-terminal acetylation: NAT 2007-2008 Symposia
    Thomas Arnesen
    Department of Molecular Biology, University of Bergen, N 5020 Bergen, Norway
    BMC Proc 3:S1. 2009
  3. pmc A synopsis of eukaryotic Nalpha-terminal acetyltransferases: nomenclature, subunits and substrates
    Bogdan Polevoda
    Department of Biochemistry and Biophysics, University of Rochester Medical Center, Rochester, NY 14642, USA
    BMC Proc 3:S2. 2009
  4. pmc The chaperone-like protein HYPK acts together with NatA in cotranslational N-terminal acetylation and prevention of Huntingtin aggregation
    Thomas Arnesen
    Department of Molecular Biology, University of Bergen, Thormøhlensgate 55, N 5020 Bergen, Norway
    Mol Cell Biol 30:1898-909. 2010
  5. pmc A novel human NatA Nalpha-terminal acetyltransferase complex: hNaa16p-hNaa10p (hNat2-hArd1)
    Thomas Arnesen
    Department of Molecular Biology, University of Bergen, N 5020 Bergen, Norway
    BMC Biochem 10:15. 2009
  6. ncbi request reprint The protein acetyltransferase ARD1: a novel cancer drug target?
    Thomas Arnesen
    Department of Molecular Biology, University of Bergen, N 5020 Bergen, Norway
    Curr Cancer Drug Targets 8:545-53. 2008
  7. pmc Towards a functional understanding of protein N-terminal acetylation
    Thomas Arnesen
    Department of Molecular Biology, University of Bergen, Bergen, Norway
    PLoS Biol 9:e1001074. 2011
  8. pmc Characterization of hARD2, a processed hARD1 gene duplicate, encoding a human protein N-alpha-acetyltransferase
    Thomas Arnesen
    Department of Molecular Biology, University of Bergen, N 5020 Bergen, Norway
    BMC Biochem 7:13. 2006
  9. ncbi request reprint Induction of apoptosis in human cells by RNAi-mediated knockdown of hARD1 and NATH, components of the protein N-alpha-acetyltransferase complex
    T Arnesen
    Department of Molecular Biology, University of Bergen, Norway
    Oncogene 25:4350-60. 2006
  10. ncbi request reprint Cloning and characterization of hNAT5/hSAN: an evolutionarily conserved component of the NatA protein N-alpha-acetyltransferase complex
    Thomas Arnesen
    Department of Molecular Biology, University of Bergen, N 5020 Bergen, Norway
    Gene 371:291-5. 2006

Collaborators

Detail Information

Publications29

  1. pmc Identification and characterization of the human ARD1-NATH protein acetyltransferase complex
    Thomas Arnesen
    Department of Molecular Biology, University of Bergen, N 5020 Bergen, Norway
    Biochem J 386:433-43. 2005
    ..This study identifies the human homologues of the yeast Ard1p and Nat1p proteins and presents new aspects of the NATH and hARD1 proteins relative to their yeast homologues...
  2. pmc Protein N-terminal acetylation: NAT 2007-2008 Symposia
    Thomas Arnesen
    Department of Molecular Biology, University of Bergen, N 5020 Bergen, Norway
    BMC Proc 3:S1. 2009
    ....
  3. pmc A synopsis of eukaryotic Nalpha-terminal acetyltransferases: nomenclature, subunits and substrates
    Bogdan Polevoda
    Department of Biochemistry and Biophysics, University of Rochester Medical Center, Rochester, NY 14642, USA
    BMC Proc 3:S2. 2009
    ..ABSTRACT : We have introduced a consistent nomenclature for the various subunits of the NatA-NatE N-terminal acetyltransferases from yeast, humans and other eukaryotes...
  4. pmc The chaperone-like protein HYPK acts together with NatA in cotranslational N-terminal acetylation and prevention of Huntingtin aggregation
    Thomas Arnesen
    Department of Molecular Biology, University of Bergen, Thormøhlensgate 55, N 5020 Bergen, Norway
    Mol Cell Biol 30:1898-909. 2010
    ..Taken together, the data indicate that the physical interaction between HYPK and NatA seems to be of functional importance both for Htt aggregation and for N-terminal acetylation...
  5. pmc A novel human NatA Nalpha-terminal acetyltransferase complex: hNaa16p-hNaa10p (hNat2-hArd1)
    Thomas Arnesen
    Department of Molecular Biology, University of Bergen, N 5020 Bergen, Norway
    BMC Biochem 10:15. 2009
    ..In humans, the homologues hNaa15p (hNat1) and hNaa10p (hArd1) were demonstrated to form a stable ribosome associated NAT complex acetylating NatA type N-termini in vitro and in vivo...
  6. ncbi request reprint The protein acetyltransferase ARD1: a novel cancer drug target?
    Thomas Arnesen
    Department of Molecular Biology, University of Bergen, N 5020 Bergen, Norway
    Curr Cancer Drug Targets 8:545-53. 2008
    ..This review focuses on the enzymatic and biological activities of hARD1, and potential mechanisms of functional inhibition...
  7. pmc Towards a functional understanding of protein N-terminal acetylation
    Thomas Arnesen
    Department of Molecular Biology, University of Bergen, Bergen, Norway
    PLoS Biol 9:e1001074. 2011
    ..These contributions represent new and intriguing hypotheses that will guide the research in the years to come...
  8. pmc Characterization of hARD2, a processed hARD1 gene duplicate, encoding a human protein N-alpha-acetyltransferase
    Thomas Arnesen
    Department of Molecular Biology, University of Bergen, N 5020 Bergen, Norway
    BMC Biochem 7:13. 2006
    ..We recently described the human protein acetyltransferase hARD1 (human Arrest Defective 1). hARD1 interacts with NATH (N-Acetyl Transferase Human) forming a complex expressing protein N-terminal alpha-acetylation activity...
  9. ncbi request reprint Induction of apoptosis in human cells by RNAi-mediated knockdown of hARD1 and NATH, components of the protein N-alpha-acetyltransferase complex
    T Arnesen
    Department of Molecular Biology, University of Bergen, Norway
    Oncogene 25:4350-60. 2006
    ..Our results argue for an essential role of the NATH-hARD1 complex in cell survival and underscore the importance of protein N-alpha-acetylation in mammalian cells...
  10. ncbi request reprint Cloning and characterization of hNAT5/hSAN: an evolutionarily conserved component of the NatA protein N-alpha-acetyltransferase complex
    Thomas Arnesen
    Department of Molecular Biology, University of Bergen, N 5020 Bergen, Norway
    Gene 371:291-5. 2006
    ..In summary, we present the first description of the human homologue of Nat5p/San, hNAT5, the third component of the human NatA N-alpha-acetyltransferase complex...
  11. pmc Knockdown of human N alpha-terminal acetyltransferase complex C leads to p53-dependent apoptosis and aberrant human Arl8b localization
    Kristian K Starheim
    Department of Molecular Biology, University of Bergen, Thormøhlensgate 55, N 5020 Bergen, Norway
    Mol Cell Biol 29:3569-81. 2009
    ..Taken together, hNatC-mediated N-terminal acetylation is important for maintenance of protein function and cell viability in human cells...
  12. doi request reprint Identification of the human N(alpha)-acetyltransferase complex B (hNatB): a complex important for cell-cycle progression
    Kristian K Starheim
    Department of Molecular Biology, University of Bergen, N 5020 Bergen, Norway
    Biochem J 415:325-31. 2008
    ..In summary, we show for the first time a vertebrate NatB protein N(alpha)-acetyltransferase complex essential for normal cell proliferation...
  13. pmc Proteomics analyses reveal the evolutionary conservation and divergence of N-terminal acetyltransferases from yeast and humans
    Thomas Arnesen
    Department of Molecular Biology, University of Bergen, Bergen, Norway
    Proc Natl Acad Sci U S A 106:8157-62. 2009
    ..Thus, although we revealed different N-acetylation patterns in yeast and humans, the major NAT, NatA, acetylates the same substrates in both species...
  14. ncbi request reprint Interaction between HIF-1 alpha (ODD) and hARD1 does not induce acetylation and destabilization of HIF-1 alpha
    Thomas Arnesen
    Department of Molecular Biology, University of Bergen, N 5020 Bergen, Norway
    FEBS Lett 579:6428-32. 2005
    ..Moreover, hARD1 does not acetylate and destabilize HIF-1 alpha. However, we find that hARD1 specifically binds HIF-1 alpha, suggesting a putative, still unclear, connection between these proteins...
  15. pmc Protein N-terminal acetyltransferases act as N-terminal propionyltransferases in vitro and in vivo
    Håvard Foyn
    Department of Molecular Biology, University of Bergen, N 5020 Bergen, Norway
    Mol Cell Proteomics 12:42-54. 2013
    ....
  16. pmc The human N-alpha-acetyltransferase 40 (hNaa40p/hNatD) is conserved from yeast and N-terminally acetylates histones H2A and H4
    Kristine Hole
    Department of Molecular Biology, University of Bergen, Bergen, Norway
    PLoS ONE 6:e24713. 2011
    ..Combined, our results strongly suggest that human Naa40p/NatD is conserved from yeast. Thus, the NATs of all classes of N-terminally acetylated proteins in humans now appear to be accounted for...
  17. doi request reprint Depletion of the human Nα-terminal acetyltransferase A induces p53-dependent apoptosis and p53-independent growth inhibition
    Darina Gromyko
    Department of Molecular Biology, University of Bergen, Bergen, Norway
    Int J Cancer 127:2777-89. 2010
    ..Our findings indicate that several mechanisms of growth inhibition and apoptosis may be induced by hNatA knockdown and that hNatA knockdown could be exploited for use in combinatorial chemotherapy...
  18. pmc Human Naa50p (Nat5/San) displays both protein N alpha- and N epsilon-acetyltransferase activity
    Rune Evjenth
    Department of Molecular Biology, University of Bergen, N 5020 Bergen, Norway
    J Biol Chem 284:31122-9. 2009
    ..In addition, histone 4 was detected as a hNaa50p KAT substrate in vitro. Our findings thus provide the first experimental evidence of an enzyme having both KAT and NAT activities...
  19. pmc Specificity and versatility of substrate binding sites in four catalytic domains of human N-terminal acetyltransferases
    Cedric Grauffel
    Department of Molecular Biology, University of Bergen, Bergen, Norway
    PLoS ONE 7:e52642. 2012
    ..Finally, by comparing the four structures we propose maps of the peptide-enzyme interactions that should help rationalizing substrate-specificity and lay the ground for inhibitor design...
  20. pmc Human protein N-terminal acetyltransferase hNaa50p (hNAT5/hSAN) follows ordered sequential catalytic mechanism: combined kinetic and NMR study
    Rune H Evjenth
    Department of Molecular Biology, University of Bergen, N 5020 Bergen, Norway
    J Biol Chem 287:10081-8. 2012
    ..This model is further strengthened by the NMR results using a catalytically inactive hNaa50p mutant...
  21. doi request reprint HPLC-based quantification of in vitro N-terminal acetylation
    Rune H Evjenth
    Department of Molecular Biology, University of Bergen, Bergen, Norway
    Methods Mol Biol 981:95-102. 2013
    ..We here describe an in vitro method based on reverse-phase HPLC to quantitatively measure in vitro acetylation of NAT oligopeptide substrates, enabling the determination of NAT specificity as well as kinetic parameters...
  22. doi request reprint Protein N-terminal acetyltransferases: when the start matters
    Kristian K Starheim
    Department of Molecular Biology, University of Bergen, Bergen, Norway
    Trends Biochem Sci 37:152-61. 2012
    ..Here, we discuss how these recent findings shed light on NATs as major protein regulators and key cellular players...
  23. pmc HIV-1 Rev oligomerization is not obligatory in the presence of an extra basic domain
    Clemens Furnes
    Department of Molecular Biology, University of Bergen, N 5020 Bergen, Norway
    Retrovirology 2:39. 2005
    ..However, the biological significance of the obligatory complex formation of Rev upon the viral RNA is unclear...
  24. pmc N-terminal acetylation by NatC is not a general determinant for substrate subcellular localization in Saccharomyces cerevisiae
    Henriette Aksnes
    Department of Molecular Biology, University of Bergen, Bergen, Norway
    PLoS ONE 8:e61012. 2013
    ..Furthermore, all organelle-localized substrates indicated undisrupted structures, thus suggesting that absence of NatC acetylation does not have a vast effect on organelle morphology in yeast...
  25. pmc Composition and biological significance of the human Nalpha-terminal acetyltransferases
    Kristian K Starheim
    Department of Molecular Biology, University of Bergen, N 5020 Bergen, Norway
    BMC Proc 3:S3. 2009
    ..We here summarize the identified N-terminal acetyltransferase complexes in humans, and we review the biological studies on Nalpha-terminal acetylation in humans and other higher eukaryotes...
  26. doi request reprint Outcome after surgery for primary hyperaldosteronism may depend on KCNJ5 tumor mutation status: a population-based study from Western Norway
    Thomas Arnesen
    Department of Surgery, Haukeland University Hospital, 5021 Bergen, Norway
    Langenbecks Arch Surg 398:869-74. 2013
    ....
  27. pmc The protein Nalpha-terminal acetyltransferase hNaa10p (hArd1) is phosphorylated in HEK293 cells
    Hiwa Målen
    Department of Molecular Biology, University of Bergen, N 5020 Bergen, Norway
    BMC Res Notes 2:32. 2009
    ..In the flexible C-terminal tail of hNaa10p, there are several potential phosphorylation sites that might serve as points of regulation...
  28. ncbi request reprint Expression of N-acetyl transferase human and human Arrest defective 1 proteins in thyroid neoplasms
    Thomas Arnesen
    Department of Surgical Sciences, University of Bergen and Haukeland University Hospital, Norway
    Thyroid 15:1131-6. 2005
    ..SiRNA-mediated knockdown of NATH resulted in decreased levels of hARD1 protein. Taken together, these results suggest that NATH positively affects the level of hARD1 protein both in vivo and in cell cultures...
  29. ncbi request reprint A novel type of deletion in the CDKN2A gene identified in a melanoma-prone family
    Stian Knappskog
    Department of Medicine, Section of Oncology, Haukeland University Hospital, N 5021 Bergen, Norway
    Genes Chromosomes Cancer 45:1155-63. 2006
    ....