Mohammed Amarzguioui

Summary

Affiliation: University of Oslo
Country: Norway

Publications

  1. ncbi request reprint An algorithm for selection of functional siRNA sequences
    Mohammed Amarzguioui
    The Biotechnology Centre of Oslo, University of Oslo, Gaustadalleen 21, Oslo N 0349, Norway
    Biochem Biophys Res Commun 316:1050-8. 2004
  2. ncbi request reprint Approaches for chemically synthesized siRNA and vector-mediated RNAi
    Mohammed Amarzguioui
    Division of Molecular Biology, Beckman Research Institute of the City of Hope, 1450 East Duarte Road, Duarte, CA 91010 3011, USA
    FEBS Lett 579:5974-81. 2005
  3. ncbi request reprint Rational design and in vitro and in vivo delivery of Dicer substrate siRNA
    Mohammed Amarzguioui
    The Biotechnology Centre of Oslo, Gaustadalleen 21, 0349 Oslo, Norway
    Nat Protoc 1:508-17. 2006
  4. ncbi request reprint Improved siRNA-mediated silencing in refractory adherent cell lines by detachment and transfection in suspension
    Mohammed Amarzguioui
    Biotechnology Centre of Oslo, University of Oslo, Oslo, Norway
    Biotechniques 36:766-8, 770. 2004
  5. ncbi request reprint Ex vivo and in vivo delivery of anti-tissue factor short interfering RNA inhibits mouse pulmonary metastasis of B16 melanoma cells
    Mohammed Amarzguioui
    The Biotechnology Centre of Oslo, Norway
    Clin Cancer Res 12:4055-61. 2006
  6. pmc Tolerance for mutations and chemical modifications in a siRNA
    Mohammed Amarzguioui
    The Biotechnology Centre of Oslo, University of Oslo, Gaustadalleen 21, N 0349, Oslo, Norway
    Nucleic Acids Res 31:589-95. 2003
  7. pmc Similar behaviour of single-strand and double-strand siRNAs suggests they act through a common RNAi pathway
    Torgeir Holen
    The Biotechnology Centre of Oslo, University of Oslo, Gaustadalleen 21, N 0349 Oslo, Norway
    Nucleic Acids Res 31:2401-7. 2003
  8. ncbi request reprint Short-interfering RNA-mediated Lck knockdown results in augmented downstream T cell responses
    Trond Methi
    The Biotechnology Centre of Oslo, University of Oslo, Oslo, Norway
    J Immunol 175:7398-406. 2005
  9. doi request reprint Principles of Dicer substrate (D-siRNA) design and function
    Mohammed Amarzguioui
    The Biotechnology Centre of Oslo, Oslo, Norway
    Methods Mol Biol 442:3-10. 2008
  10. ncbi request reprint Downregulation of tissue factor by RNA interference in human melanoma LOX-L cells reduces pulmonary metastasis in nude mice
    Xiaolin Wang
    Biotechnology Center of Oslo, University of Oslo, Oslo, Norway
    Int J Cancer 112:994-1002. 2004

Collaborators

Detail Information

Publications15

  1. ncbi request reprint An algorithm for selection of functional siRNA sequences
    Mohammed Amarzguioui
    The Biotechnology Centre of Oslo, University of Oslo, Gaustadalleen 21, Oslo N 0349, Norway
    Biochem Biophys Res Commun 316:1050-8. 2004
    ..A selection algorithm based on these findings strongly differentiated between the two functional groups of siRNA in both data sets and proved highly effective when used to design siRNA targeting new endogenous human genes...
  2. ncbi request reprint Approaches for chemically synthesized siRNA and vector-mediated RNAi
    Mohammed Amarzguioui
    Division of Molecular Biology, Beckman Research Institute of the City of Hope, 1450 East Duarte Road, Duarte, CA 91010 3011, USA
    FEBS Lett 579:5974-81. 2005
    ....
  3. ncbi request reprint Rational design and in vitro and in vivo delivery of Dicer substrate siRNA
    Mohammed Amarzguioui
    The Biotechnology Centre of Oslo, Gaustadalleen 21, 0349 Oslo, Norway
    Nat Protoc 1:508-17. 2006
    ..siRNA delivery and transfection and analysis of macrophages in vivo can be accomplished within 36 h...
  4. ncbi request reprint Improved siRNA-mediated silencing in refractory adherent cell lines by detachment and transfection in suspension
    Mohammed Amarzguioui
    Biotechnology Centre of Oslo, University of Oslo, Oslo, Norway
    Biotechniques 36:766-8, 770. 2004
  5. ncbi request reprint Ex vivo and in vivo delivery of anti-tissue factor short interfering RNA inhibits mouse pulmonary metastasis of B16 melanoma cells
    Mohammed Amarzguioui
    The Biotechnology Centre of Oslo, Norway
    Clin Cancer Res 12:4055-61. 2006
    ..The purposes of this work are to establish a noncytotoxic in vivo model for investigation of tissue factor function and provide preclinical assessment of the therapeutic potential of tissue factor siRNA for prevention of metastasis...
  6. pmc Tolerance for mutations and chemical modifications in a siRNA
    Mohammed Amarzguioui
    The Biotechnology Centre of Oslo, University of Oslo, Gaustadalleen 21, N 0349, Oslo, Norway
    Nucleic Acids Res 31:589-95. 2003
    ..Most of the chemically modified versions tested had near-wild-type initial activity, while the long-term activity was increased for certain siRNA species. Our results may improve the design of siRNAs for in vivo experiments...
  7. pmc Similar behaviour of single-strand and double-strand siRNAs suggests they act through a common RNAi pathway
    Torgeir Holen
    The Biotechnology Centre of Oslo, University of Oslo, Gaustadalleen 21, N 0349 Oslo, Norway
    Nucleic Acids Res 31:2401-7. 2003
    ....
  8. ncbi request reprint Short-interfering RNA-mediated Lck knockdown results in augmented downstream T cell responses
    Trond Methi
    The Biotechnology Centre of Oslo, University of Oslo, Oslo, Norway
    J Immunol 175:7398-406. 2005
    ..This report underscores Lck as an important mediator of proximal TCR signaling, but also indicates a suppressive role on downstream immune function...
  9. doi request reprint Principles of Dicer substrate (D-siRNA) design and function
    Mohammed Amarzguioui
    The Biotechnology Centre of Oslo, Oslo, Norway
    Methods Mol Biol 442:3-10. 2008
    ..We describe the fundamental principles and experimental results leading to optimal Dicer substrates...
  10. ncbi request reprint Downregulation of tissue factor by RNA interference in human melanoma LOX-L cells reduces pulmonary metastasis in nude mice
    Xiaolin Wang
    Biotechnology Center of Oslo, University of Oslo, Oslo, Norway
    Int J Cancer 112:994-1002. 2004
    ..The results provide a basis for investigating molecular mechanisms underlying the effects of TF on the metastatic capacity of LOX-L melanoma cells...
  11. pmc Positional effects of short interfering RNAs targeting the human coagulation trigger Tissue Factor
    Torgeir Holen
    The Biotechnology Centre of Oslo, University of Oslo, Gaustadalleen 21, N 0349 Oslo, Norway
    Nucleic Acids Res 30:1757-66. 2002
    ..Finally, we observed 3' mRNA cleavage fragments resulting from the action of the most effective siRNAs. The depletion rate-dependent appearance of these fragments argues for the existence of a two-step mRNA degradation mechanism...
  12. ncbi request reprint Knockdown of C-terminal Src kinase by siRNA-mediated RNA interference augments T cell receptor signaling in mature T cells
    Torkel Vang
    The Biotechnology Center of Oslo, University of Oslo, Blindern, N 0317 Oslo, Norway
    Eur J Immunol 34:2191-9. 2004
    ..Altogether, these data suggest that acute elimination of Csk in T cells without evolution of compensatory mechanisms results in aberrant Lck activity and augmented TCR-stimulated responses...
  13. ncbi request reprint A facile lentiviral vector system for expression of doxycycline-inducible shRNAs: knockdown of the pre-miRNA processing enzyme Drosha
    Lars Aagaard
    Division of Molecular Biology, Beckman Research Institute of the City of Hope, Duarte, California 91010, USA
    Mol Ther 15:938-45. 2007
    ..We anticipate that our vector will facilitate functional studies of miRNA biogenesis...
  14. pmc Functional polarity is introduced by Dicer processing of short substrate RNAs
    Scott D Rose
    Integrated DNA Technologies, Inc 1710 Commercial Park, Coralville, IA 52241, USA
    Nucleic Acids Res 33:4140-56. 2005
    ..Thus, Dicer processing confers functional polarity within the RNAi pathway...
  15. pmc The antisense strand of small interfering RNAs directs histone methylation and transcriptional gene silencing in human cells
    Marc S Weinberg
    Department of Molecular Medicine and Hematology, University of the Witwatersrand Medical School, Parktown, South Africa
    RNA 12:256-62. 2006
    ....