H Stenmark

Summary

Affiliation: The Norwegian Radium Hospital
Country: Norway

Publications

  1. pmc Hrs regulates multivesicular body formation via ESCRT recruitment to endosomes
    Kristi G Bache
    Department of Biochemistry, The Norwegian Radium Hospital, Montebello, N 0310 Oslo, Norway
    J Cell Biol 162:435-42. 2003
  2. ncbi request reprint Intracellular trafficking and turnover of phosphatidylinositol 3-phosphate
    H Stenmark
    Department of Biochemistry, The Norwegian Radium Hospital, Montebello, N 0310 Oslo, Norway
    Semin Cell Dev Biol 12:193-9. 2001
  3. pmc The Rab GTPase family
    H Stenmark
    Department of Biochemistry, Norwegian Radium Hospital, Montebello, N 0310 Oslo, Norway
    Genome Biol 2:REVIEWS3007. 2001
  4. doi request reprint Rab GTPases as coordinators of vesicle traffic
    Harald Stenmark
    Department of Biochemistry, Institute for Cancer Research, The Norwegian Radium Hospital, University of Oslo, Montebello, N 0310 Oslo, Norway
    Nat Rev Mol Cell Biol 10:513-25. 2009
  5. ncbi request reprint Hrs sorts ubiquitinated proteins into clathrin-coated microdomains of early endosomes
    Camilla Raiborg
    Department of Biochemistry, Institute for Cancer Research, The Norwegian Radium Hospital, Montebello, N 0310 Oslo, Norway
    Nat Cell Biol 4:394-8. 2002
  6. pmc The Sir Hans Krebs Lecture. How a lipid mediates tumour suppression. Delivered on 29 June 2010 at the 35th FEBS Congress in Gothenburg, Sweden
    Harald Stenmark
    Centre for Cancer Biomedicine, Faculty of Medicine, University of Oslo, Norway
    FEBS J 277:4837-48. 2010
  7. ncbi request reprint Cycling lipids
    H Stenmark
    Department of Biochemistry, The Norwegian Radium Hospital, Oslo, N 0310, Norway
    Curr Biol 10:R57-9. 2000
  8. ncbi request reprint FYVE and coiled-coil domains determine the specific localisation of Hrs to early endosomes
    C Raiborg
    Department of Biochemistry, Institute for Cancer Research, The Norwegian Radium Hospital, Montebello, N 0310 Oslo, Norway
    J Cell Sci 114:2255-63. 2001
  9. pmc Hrs recruits clathrin to early endosomes
    C Raiborg
    Department of Biochemistry, Institute for Cancer Research, The Norwegian Radium Hospital, Montebello, N 0310 Oslo, Norway
    EMBO J 20:5008-21. 2001
  10. ncbi request reprint Direct interaction of EEA1 with Rab5b
    J Callaghan
    Department of Biochemistry, The Norwegian Radium Hospital, Oslo
    Eur J Biochem 265:361-6. 1999

Collaborators

Detail Information

Publications102 found, 100 shown here

  1. pmc Hrs regulates multivesicular body formation via ESCRT recruitment to endosomes
    Kristi G Bache
    Department of Biochemistry, The Norwegian Radium Hospital, Montebello, N 0310 Oslo, Norway
    J Cell Biol 162:435-42. 2003
    ..We conclude that Hrs mediates the initial recruitment of ESCRT-I to endosomes and, thereby, indirectly regulates multivesicular body formation...
  2. ncbi request reprint Intracellular trafficking and turnover of phosphatidylinositol 3-phosphate
    H Stenmark
    Department of Biochemistry, The Norwegian Radium Hospital, Montebello, N 0310 Oslo, Norway
    Semin Cell Dev Biol 12:193-9. 2001
    ....
  3. pmc The Rab GTPase family
    H Stenmark
    Department of Biochemistry, Norwegian Radium Hospital, Montebello, N 0310 Oslo, Norway
    Genome Biol 2:REVIEWS3007. 2001
    ..In the GTP-bound form, the Rab GTPases recruit specific sets of effector proteins onto membranes. Through their effectors, Rab GTPases regulate vesicle formation, actin- and tubulin-dependent vesicle movement, and membrane fusion...
  4. doi request reprint Rab GTPases as coordinators of vesicle traffic
    Harald Stenmark
    Department of Biochemistry, Institute for Cancer Research, The Norwegian Radium Hospital, University of Oslo, Montebello, N 0310 Oslo, Norway
    Nat Rev Mol Cell Biol 10:513-25. 2009
    ..Functional impairments of Rab pathways are associated with diseases, such as immunodeficiencies, cancer and neurological disorders...
  5. ncbi request reprint Hrs sorts ubiquitinated proteins into clathrin-coated microdomains of early endosomes
    Camilla Raiborg
    Department of Biochemistry, Institute for Cancer Research, The Norwegian Radium Hospital, Montebello, N 0310 Oslo, Norway
    Nat Cell Biol 4:394-8. 2002
    ..We conclude that Hrs sorts ubiquitinated membrane proteins into clathrin-coated microdomains of early endosomes, thereby preventing their recycling to the cell surface...
  6. pmc The Sir Hans Krebs Lecture. How a lipid mediates tumour suppression. Delivered on 29 June 2010 at the 35th FEBS Congress in Gothenburg, Sweden
    Harald Stenmark
    Centre for Cancer Biomedicine, Faculty of Medicine, University of Oslo, Norway
    FEBS J 277:4837-48. 2010
    ....
  7. ncbi request reprint Cycling lipids
    H Stenmark
    Department of Biochemistry, The Norwegian Radium Hospital, Oslo, N 0310, Norway
    Curr Biol 10:R57-9. 2000
    ..To form new synaptic vesicles, the clathrin coat must be shed and recent studies reveal that a lipid phosphatase, synaptojanin, plays a central role in this process...
  8. ncbi request reprint FYVE and coiled-coil domains determine the specific localisation of Hrs to early endosomes
    C Raiborg
    Department of Biochemistry, Institute for Cancer Research, The Norwegian Radium Hospital, Montebello, N 0310 Oslo, Norway
    J Cell Sci 114:2255-63. 2001
    ..We speculate that binding to PtdIns(3)P and a SNAP-25-related molecule may target Hrs specifically to early endosomes...
  9. pmc Hrs recruits clathrin to early endosomes
    C Raiborg
    Department of Biochemistry, Institute for Cancer Research, The Norwegian Radium Hospital, Montebello, N 0310 Oslo, Norway
    EMBO J 20:5008-21. 2001
    ..These results provide a molecular mechanism for the recruitment of clathrin onto early endosomes and suggest a function for Hrs in trafficking from early to late endosomes...
  10. ncbi request reprint Direct interaction of EEA1 with Rab5b
    J Callaghan
    Department of Biochemistry, The Norwegian Radium Hospital, Oslo
    Eur J Biochem 265:361-6. 1999
    ..These data provide evidence that EEA1 interacts with both Rab5a and Rab5b, and that the GTPase activities of the two proteins are differentially regulated in vivo...
  11. ncbi request reprint Interaction of the EEA1 FYVE finger with phosphatidylinositol 3-phosphate and early endosomes. Role of conserved residues
    J M Gaullier
    Department of Biochemistry, The Norwegian Radium Hospital, Montebello, N 0310 Oslo, Norway
    J Biol Chem 275:24595-600. 2000
    ..These results support recently proposed models for the ligand binding of the FYVE domain and indicate that PtdIns(3)P binding is crucial for the localization and function of EEA1...
  12. pmc The endosome fusion regulator early-endosomal autoantigen 1 (EEA1) is a dimer
    J Callaghan
    Department of Biochemistry, The Norwegian Radium Hospital, Montebello, N 0310 Oslo, Norway
    Biochem J 338:539-43. 1999
    ..These data enable us to propose a model for the quaternary structure of EEA1...
  13. ncbi request reprint The Rab5 effector EEA1 interacts directly with syntaxin-6
    A Simonsen
    Department of Biochemistry, The Norwegian Radium Hospital, Montebello, N 0310 Oslo, Norway
    J Biol Chem 274:28857-60. 1999
    ..Our results indicate that SNAREs can interact directly with Rab effectors, and suggest that EEA1 may participate in trans-Golgi network to endosome as well as in endocytic membrane traffic...
  14. ncbi request reprint Endosomal localization of the autoantigen EEA1 is mediated by a zinc-binding FYVE finger
    H Stenmark
    Department of Biochemistry, The Norwegian Radium Hospital, Montebello, N 0310 Oslo, Norway
    J Biol Chem 271:24048-54. 1996
    ..These results implicate the FYVE finger in the specific localization of EEA1 to endosomes...
  15. ncbi request reprint Function of Hrs in endocytic trafficking and signalling
    C Raiborg
    Department of Biochemistry, Norwegian Radium Hospital, Montebello, N-0310 Oslo, Norway
    Biochem Soc Trans 29:472-5. 2001
    ..Here we discuss the possible functions of Hrs in light of its interactions with phosphatidylinositol 3-phosphate and multiple proteins...
  16. pmc Phosphoinositides and phagocytosis
    D J Gillooly
    Department of Biochemistry, Institute for Cancer Research, The Norwegian Radium Hospital, Montebello, N-0310 Oslo, Norway
    J Cell Biol 155:15-7. 2001
    ..This suggests a possible mechanism of how this microorganism evades degradation in phagolysosomes...
  17. pmc Localization of phosphatidylinositol 3-phosphate in yeast and mammalian cells
    D J Gillooly
    Department of Biochemistry, Institute for Cancer Research, The Norwegian Radium Hospital, Montebello, 0310 Oslo, Norway
    EMBO J 19:4577-88. 2000
    ....
  18. ncbi request reprint The role of phosphoinositides in membrane transport
    A Simonsen
    Department of Biochemistry, Institute for Cancer Research, The Norwegian Radium Hospital, Montebello, N-0310, Oslo, Norway
    Curr Opin Cell Biol 13:485-92. 2001
    ....
  19. pmc Cellular functions of phosphatidylinositol 3-phosphate and FYVE domain proteins
    D J Gillooly
    Department of Biochemistry, The Norwegian Radium Hospital, Montebello, 0310 Oslo, Norway
    Biochem J 355:249-58. 2001
    ....
  20. ncbi request reprint Protein targeting to endosomes and phagosomes via FYVE and PX domains
    H C G Birkeland
    Department of Biochemistry, Norwegian Radium Hospital, Montebello, 0310 Oslo, Norway
    Curr Top Microbiol Immunol 282:89-115. 2004
    ....
  21. ncbi request reprint EEA1 links PI(3)K function to Rab5 regulation of endosome fusion
    A Simonsen
    Department of Biochemistry, The Norwegian Radium Hospital, Montebello, Oslo
    Nature 394:494-8. 1998
    ....
  22. doi request reprint PtdIns(3)P controls cytokinesis through KIF13A-mediated recruitment of FYVE-CENT to the midbody
    Antonia P Sagona
    Centre for Cancer Biomedicine, Faculty of Medicine, University of Oslo, Montebello, N 0310 Oslo, Norway
    Nat Cell Biol 12:362-71. 2010
    ..These results provide a mechanism for the translocation and docking of a cytokinesis regulatory machinery at the midbody...
  23. ncbi request reprint Alix regulates cortical actin and the spatial distribution of endosomes
    Alicia Cabezas
    Department of Biochemistry, The Norwegian Radium Hospital, Montebello, Oslo
    J Cell Sci 118:2625-35. 2005
    ....
  24. ncbi request reprint Flat clathrin coats on endosomes mediate degradative protein sorting by scaffolding Hrs in dynamic microdomains
    Camilla Raiborg
    Department of Biochemistry, Institute for Cancer Research, the Norwegian Radium Hospital and the University of Oslo, Montebello, N 0310 Oslo, Norway
    J Cell Sci 119:2414-24. 2006
    ..We conclude that clathrin is responsible for concentrating Hrs in endosomal microdomains specialised for recognition of ubiquitylated membrane proteins, thus enabling efficient sorting of cargo into the degradative pathway...
  25. ncbi request reprint Insertion of diphtheria toxin B-fragment into the plasma membrane at low pH. Characterization and topology of inserted regions
    J O Moskaug
    Institute for Cancer Research, Norwegian Radium Hospital, Montebello, Oslo
    J Biol Chem 266:2652-9. 1991
    ..The topology of the inserted B-fragment is discussed...
  26. pmc Cloning of an intracellular protein that binds selectively to mitogenic acidic fibroblast growth factor
    E Kolpakova
    Institute for Cancer Research, The Norwegian Radium Hospital, Montebello, 0310 Oslo, Norway
    Biochem J 336:213-22. 1998
    ..The possibility is discussed that FIBP may be involved in the mitogenic action of aFGF...
  27. ncbi request reprint Alfy, a novel FYVE-domain-containing protein associated with protein granules and autophagic membranes
    Anne Simonsen
    Department of Biochemistry, The Norwegian Radium Hospital, Montebello, Oslo 0310, Norway
    J Cell Sci 117:4239-51. 2004
    ..By electron microscopy, similar structures can be found within autophagosomes. We propose that Alfy might target cytosolic protein aggregates for autophagic degradation...
  28. ncbi request reprint The structure of an endosomal protein sorter
    Thomas Slagsvold
    Department of Biochemistry, The Norwegian Radium Hospital, Montebello, N 0310 Oslo, Norway
    Dev Cell 7:457-8. 2004
    ..Two recent reports, one in Nature and one in this issue of Developmental Cell, reveal the crystal structure of the yeast form of ESCRT-II...
  29. doi request reprint ESCRT & Co
    Ingrid Roxrud
    Centre for Cancer Biomedicine, Faculty of Medicine, University of Oslo, Montebello, N 0310 Oslo, Norway
    Biol Cell 102:293-318. 2010
    ..In conclusion, the discovery of novel interactors has and will extend our knowledge of the biological roles of ESCRTs...
  30. ncbi request reprint Endosomal and non-endosomal functions of ESCRT proteins
    Thomas Slagsvold
    Department of Biochemistry, the Norwegian Radium Hospital and the University of Oslo, Montebello, N 0310 Oslo, Norway
    Trends Cell Biol 16:317-26. 2006
    ..We also discuss evidence that some ESCRT subunits have evolved additional functions that are unrelated to membrane trafficking...
  31. pmc An endosomally localized isoform of Eps15 interacts with Hrs to mediate degradation of epidermal growth factor receptor
    Ingrid Roxrud
    Centre for Cancer Biomedicine, University of Oslo and The Norwegian Radium Hospital, Montebello, N 0310 Oslo, Norway
    J Cell Biol 180:1205-18. 2008
    ..These results indicate that Eps15b is an endosomally localized isoform of Eps15 that is present in the Hrs complex via direct Hrs interaction and important for the sorting function of this complex...
  32. ncbi request reprint Cloning and subcellular localization of a human phosphatidylinositol 3-phosphate 5-kinase, PIKfyve/Fab1
    Alicia Cabezas
    Department of Biochemistry, The Norwegian Radium Hospital, Montebello, N 0310 Oslo, Norway
    Gene 371:34-41. 2006
    ..We show that PIKfyve is distributed in microdomains that are distinct from those occupied by EEA1 and Hrs...
  33. pmc Cell-cycle-dependent binding kinetics for the early endosomal tethering factor EEA1
    Trygve Bergeland
    Department of Molecular Biosciences, University of Oslo, PO Box 1041, Blindernveien 31, Oslo 0316, Norway
    EMBO Rep 9:171-8. 2008
    ..This might be a general mechanism to fine-tune and control tethering and fusion of early endosomes...
  34. ncbi request reprint STAM and Hrs are subunits of a multivalent ubiquitin-binding complex on early endosomes
    Kristi G Bache
    Department of Biochemistry, Institute for Cancer Research, The Norwegian Radium Hospital, Montebello, N 0310 Oslo, Norway
    J Biol Chem 278:12513-21. 2003
    ..We propose that Hrs, Eps15, and STAM proteins function in a multivalent complex that sorts ubiquitinated proteins into the multivesicular body pathway...
  35. ncbi request reprint Hrs and endocytic sorting of ubiquitinated membrane proteins
    Camilla Raiborg
    Department of Biochemistry, Institute for Cancer Research, Norwegian Radium Hospital, Montebello, N 0310 Oslo, Norway
    Cell Struct Funct 27:403-8. 2002
    ....
  36. pmc SPG20, a novel biomarker for early detection of colorectal cancer, encodes a regulator of cytokinesis
    G E Lind
    Department of Cancer Prevention, Institute for Cancer Research, Radiumhospitalet, Oslo University Hospital, Oslo, Norway
    Oncogene 30:3967-78. 2011
    ..The present study identifies SPG20 promoter hypermethylation as a biomarker suitable for non-invasive detection of colorectal cancer, and a possible mechanism for cytokinesis arrest in colorectal tumorigenesis...
  37. ncbi request reprint Phosphorylation of Hrs downstream of the epidermal growth factor receptor
    Kristi G Bache
    Department of Biochemistry, Institute for Cancer Research, The Norwegian Radium Hospital, Montebello, Oslo, Norway
    Eur J Biochem 269:3881-7. 2002
    ..These results suggest that Hrs is a substrate for several kinases downstream of the EGF receptor...
  38. ncbi request reprint Protein sorting into multivesicular endosomes
    Camilla Raiborg
    Department of Biochemistry, Institute for Cancer Research, The Norwegian Radium Hospital, Montebello, N 0310, Oslo, Norway
    Curr Opin Cell Biol 15:446-55. 2003
    ..Enveloped RNA viruses such as HIV can redirect these complexes from multivesicular endosomes to the plasma membrane to facilitate viral budding...
  39. ncbi request reprint Eap45 in mammalian ESCRT-II binds ubiquitin via a phosphoinositide-interacting GLUE domain
    Thomas Slagsvold
    Department of Biochemistry, The Norwegian Radium Hospital, Montebello, Oslo
    J Biol Chem 280:19600-6. 2005
    ..Furthermore, we show that Eap45 colocalizes with ubiquitinated proteins on late endosomes. These results are consistent with a role for Eap45 in endosomal sorting of ubiquitinated cargo...
  40. ncbi request reprint Both clathrin-positive and -negative coats are involved in endosomal sorting of the EGF receptor
    Frøydis D Myromslien
    Institute of Pathology, University of Oslo, Rikshospitalet Radiumhospitalet HF, 0027 Oslo, Norway
    Exp Cell Res 312:3036-48. 2006
    ..These results demonstrate that both clathrin-positive and clathrin-negative electron dense coats exist on endosomes and are involved in endosomal sorting of the EGFR...
  41. ncbi request reprint Phosphatidylinositol 3-phosphate is found in microdomains of early endosomes
    David J Gillooly
    Department of Biochemistry, Institute for Cancer Research, The Norwegian Radium Hospital, Montebello, 0310 Oslo, Norway
    Histochem Cell Biol 120:445-53. 2003
    ..These microdomains may be necessary for the assembly of distinct multimolecular complexes that specify organelle identity, membrane trafficking and receptor signalling...
  42. doi request reprint The ESCRT machinery in endosomal sorting of ubiquitylated membrane proteins
    Camilla Raiborg
    Centre for Cancer Biomedicine, Norwegian Radium Hospital, University of Oslo, Oslo, Norway
    Nature 458:445-52. 2009
    ..The involvement of the ESCRT machinery in suppressing diseases such as cancer, neurodegeneration and infections underscores its importance to the cell...
  43. doi request reprint ESCRT proteins in physiology and disease
    Susanne Stuffers
    Centre for Cancer Biomedicine, Faculty Division, The Norwegian Radium Hospital, University of Oslo, Norway
    Exp Cell Res 315:1619-26. 2009
    ..We also review the additional functions of ESCRT proteins in cytokinesis and discuss how these may be related to ESCRT-associated pathologies...
  44. ncbi request reprint A new side to ubiquitin
    Camilla Raiborg
    Department of Biochemistry, the Norwegian Radium Hospital and the University of Oslo, Montebello, N 0310 Oslo, Norway
    Trends Biochem Sci 31:541-4. 2006
    ..The discovery of an alternative interaction face of ubiquitin opens new avenues for understanding how this small protein regulates protein function...
  45. ncbi request reprint Vps22/EAP30 in ESCRT-II mediates endosomal sorting of growth factor and chemokine receptors destined for lysosomal degradation
    Lene Malerød
    Centre for Cancer Biomedicine, University of Oslo, Montebello, N 0310 Oslo, Norway
    Traffic 8:1617-29. 2007
    ..In contrast, this was not the case when Vps22 was depleted. These results indicate an important role for Vps22 in ligand-induced EGFR and CXCR4 turnover and suggest that termination of EGF signaling occurs prior to ESCRT-II engagement...
  46. pmc Fab1 phosphatidylinositol 3-phosphate 5-kinase controls trafficking but not silencing of endocytosed receptors
    Tor Erik Rusten
    Department of Biochemistry, the Norwegian Radium Hospital and the University of Oslo, Montebello, N 0310 Oslo, Norway
    Mol Biol Cell 17:3989-4001. 2006
    ..This is consistent with the possibility that Fab1 functions at a late stage in endocytic receptor trafficking, at a point when signal termination has occurred...
  47. ncbi request reprint Analyzing phosphoinositides and their interacting proteins
    Tor Erik Rusten
    Department of Biochemistry, the Norwegian Radium Hospital and the University of Oslo, Montebello, N 0310 Oslo, Norway
    Nat Methods 3:251-8. 2006
    ..Here we describe strategies and techniques for quantitative and qualitative measurement of PIs, for characterization of specific PI-binding proteins and for determination of PI kinase and phosphatase activities in vitro and in vivo...
  48. doi request reprint The role of ESCRT proteins in attenuation of cell signalling
    Lina M Rodahl
    Centre for Cancer Biomedicine, Faculty Division, Norwegian Radium Hospital, University of Oslo, N 0310 Oslo, Norway
    Biochem Soc Trans 37:137-42. 2009
    ....
  49. ncbi request reprint Regulation of membrane traffic by phosphoinositide 3-kinases
    Karine Lindmo
    Department of Biochemistry, Norwegian Radium Hospital and University of Oslo, Montebello, N 0310 Oslo, Norway
    J Cell Sci 119:605-14. 2006
    ..By contrast, class III PI 3-kinases mainly mediate receptor-independent trafficking events, which mostly are related to endocytic membrane traffic, phagosome maturation and autophagy...
  50. pmc Defective downregulation of receptor tyrosine kinases in cancer
    Kristi G Bache
    Department of Biochemistry, Norwegian Radium Hospital, Montebello, Oslo, Norway
    EMBO J 23:2707-12. 2004
    ..A complex molecular machinery that uses the small protein ubiquitin as a key regulator assures proper endocytosis and degradation of RTKs. Here we discuss evidence that implicates deregulation of this machinery in cancer...
  51. pmc The selective macroautophagic degradation of aggregated proteins requires the PI3P-binding protein Alfy
    Maria Filimonenko
    Centre for Cancer Biomedicine, University of Oslo, Department of Biochemistry, The Norwegian Radium Hospital, Oslo, Norway
    Mol Cell 38:265-79. 2010
    ..We propose that Alfy plays a key role in selective macroautophagy by bridging cargo to the molecular machinery that builds autophagosomes...
  52. pmc Functional multivesicular bodies are required for autophagic clearance of protein aggregates associated with neurodegenerative disease
    Maria Filimonenko
    Centre for Cancer Biomedicine, University of Oslo and Department of Biochemistry, The Norwegian Radium Hospital, Montebello, N 0310 Oslo, Norway
    J Cell Biol 179:485-500. 2007
    ..Together, our data indicate that efficient autophagic degradation requires functional MVBs and provide a possible explanation to the observed neurodegenerative phenotype seen in patients with CHMP2B mutations...
  53. doi request reprint Ubiquitylation of the gap junction protein connexin-43 signals its trafficking from early endosomes to lysosomes in a process mediated by Hrs and Tsg101
    Edward Leithe
    Centre for Cancer Biomedicine, Faculty of Medicine, University of Oslo and Institute for Cancer Research, Norwegian Radium Hospital, Oslo University Hospital, Montebello, Oslo, Norway
    J Cell Sci 122:3883-93. 2009
    ..Collectively, these data reveal a central role of early endosomes in sorting of ubiquitylated Cx43, and identify Hrs and Tsg101 as crucial regulators of trafficking of Cx43 to lysosomes...
  54. doi request reprint Ubiquitination of alpha 5 beta 1 integrin controls fibroblast migration through lysosomal degradation of fibronectin-integrin complexes
    Viola Hélène Lobert
    Centre for Cancer Biomedicine, Faculty of Medicine, University of Oslo, 0316 Oslo, Norway
    Dev Cell 19:148-59. 2010
    ..Fibronectin and alpha 5 beta 1 integrin therefore are trafficked to lysosomes in a similar way to growth factors and their receptors...
  55. pmc The growth-regulatory protein HCRP1/hVps37A is a subunit of mammalian ESCRT-I and mediates receptor down-regulation
    Kristi G Bache
    Department of Biochemistry, The Norwegian Radium Hospital, Montebello, N 0310 Oslo, Norway
    Mol Biol Cell 15:4337-46. 2004
    ..Together, these results indicate that HCRP1 is a subunit of mammalian ESCRT-I and that its function is essential for lysosomal sorting of EGF receptors...
  56. doi request reprint Cytokinesis and cancer
    Antonia P Sagona
    Centre for Cancer Biomedicine, Faculty of Medicine, University of Oslo, Oslo, Norway
    FEBS Lett 584:2652-61. 2010
    ..In this minireview, we will discuss the possible links between cytokinesis and cancer, and highlight key mechanisms that connect these processes...
  57. ncbi request reprint Peroxisomal targeting as a tool for assaying potein-protein interactions in the living cell: cytokine-independent survival kinase (CISK) binds PDK-1 in vivo in a phosphorylation-dependent manner
    Trine Nilsen
    Department of Biochemistry, Institute for Cancer Research, The Norwegian Radium Hospital, Montebello, 0310 Oslo, Norway
    J Biol Chem 279:4794-801. 2004
    ..The method therefore allows assessment of protein-protein interactions that depend on post-translational modifications that are cell-specific or dependent on the physiological state of the cell...
  58. pmc CISK attenuates degradation of the chemokine receptor CXCR4 via the ubiquitin ligase AIP4
    Thomas Slagsvold
    Department of Biochemistry, the Norwegian Radium Hospital and the University of Oslo, Montebello, Oslo, Norway
    EMBO J 25:3738-49. 2006
    ..Taken together, these results reveal a critical function of CISK in specifically attenuating ubiquitin-dependent degradation of CXCR4, and provide a mechanistic link between the PI 3-kinase pathway and CXCR4 stability...
  59. ncbi request reprint The PI 3-kinase regulator Vps15 is required for autophagic clearance of protein aggregates
    Karine Lindmo
    Centre for Cancer Biomedicine, University of Oslo, Institute for Cancer Research, The Norwegian Radium Hospital, Montebello, Oslo, Norway
    Autophagy 4:500-6. 2008
    ..These results provide the first genetic evidence for a function of Vps15 in autophagy in multicellular organisms and suggest that the Vps15-containing PI 3-kinase complex may play an important role in clearance of protein aggregates...
  60. ncbi request reprint Differential functions of Hrs and ESCRT proteins in endocytic membrane trafficking
    Camilla Raiborg
    Centre for Cancer Biomedicine, University of Oslo, and Department of Biochemistry, The Norwegian Radium Hospital, Rikshospitalet, Norway
    Exp Cell Res 314:801-13. 2008
    ....
  61. doi request reprint Divide and ProsPer: the emerging role of PtdIns3P in cytokinesis
    Ioannis P Nezis
    Centre for Cancer Biomedicine, Faculty of Medicine, University of Oslo, Montebello, Oslo, Norway
    Trends Cell Biol 20:642-9. 2010
    ..Elucidating the role of PtdIns3P in cytokinesis might contribute to insight into mechanisms of tumour development and suppression...
  62. pmc Ref(2)P, the Drosophila melanogaster homologue of mammalian p62, is required for the formation of protein aggregates in adult brain
    Ioannis P Nezis
    Department of Biochemistry, Centre for Cancer Biomedicine, University of Oslo and Institute for Cancer Research, The Norwegian Radium Hospital, N 0310 Oslo, Norway
    J Cell Biol 180:1065-71. 2008
    ..Our findings reveal a major role for Ref(2)P in the formation of ubiquitin-positive protein aggregates both under physiological conditions and when normal protein turnover is inhibited...
  63. pmc Self-eating from an ER-associated cup
    Anne Simonsen
    Centre for Cancer Biomedicine, Faculty of Medicine, University of Oslo, Oslo, Norway
    J Cell Biol 182:621-2. 2008
    ..Griffiths, and N.T. Ktistakis. 2008. J. Cell Biol. 182:685-701) provides evidence that cup-shaped protrusions from the endoplasmic reticulum, named omegasomes, serve as platforms for autophagosome biogenesis in mammalian cells...
  64. doi request reprint Cindr interacts with anillin to control cytokinesis in Drosophila melanogaster
    Kaisa Haglund
    Department of Biochemistry, Institute for Cancer Research, Oslo University Hospital, N 0310 Oslo, Norway
    Curr Biol 20:944-50. 2010
    ..We propose that Cindr and Anillin cooperate to promote intercellular bridge stability during incomplete cytokinesis in Drosophila melanogaster...
  65. pmc Disruption of Vps4 and JNK function in Drosophila causes tumour growth
    Lina M Rodahl
    Faculty of Medicine, Centre for Cancer Biomedicine, University of Oslo and Institute for Cancer Research, The Norwegian Radium Hospital, Rikshospitalet University Hospital, Montebello, Oslo, Norway
    PLoS ONE 4:e4354. 2009
    ..We conclude that dvps4 is a tumour suppressor in Drosophila and that JNK is central to the cell-autonomous phenotypes of ESCRT-deficient cells...
  66. doi request reprint Multivesicular endosome biogenesis in the absence of ESCRTs
    Susanne Stuffers
    Department of Biochemistry, Institute for Cancer Research, The Norwegian Radiumhospital and Centre for Cancer Biomedicine, Faculty of Medicine, University of Oslo, Montebello, N 0310 Oslo, Norway
    Traffic 10:925-37. 2009
    ..Our observations suggest that both ESCRT-dependent and ESCRT-independent mechanisms of MVE biogenesis exist in mammalian cells...
  67. pmc Time-resolved ultrastructural detection of phosphatidylinositol 3-phosphate
    Susanne Stuffers
    Dept of Biochemistry, Institute for Cancer Research, the Norwegian Radium Hospital and Centre for Cancer Biomedicine, Faculty of Medicine, University of Oslo, Montebello, Oslo, Norway
    J Histochem Cytochem 58:1025-32. 2010
    ..We conclude that the dimerizer-inducible probe is useful for the time-resolved detection of PtdIns(3)P at the ultrastructural level, but its effects on endosome morphology after EGF stimulation need to be taken into account...
  68. doi request reprint How do ESCRT proteins control autophagy?
    Tor Erik Rusten
    Centre for Cancer Biomedicine, Faculty of Medicine, University of Oslo, Montebello, N 0310 Oslo, Norway
    J Cell Sci 122:2179-83. 2009
    ..We propose that the involvement of ESCRT proteins in the fusion of autophagosomes with the endolysosomal system is the most plausible model...
  69. doi request reprint Autophagy in tumour suppression and promotion
    Andreas Brech
    Centre for Cancer Biomedicine, Faculty of Medicine, University of Oslo, Montebello, N 0310 Oslo, Norway
    Mol Oncol 3:366-75. 2009
    ..In this review we discuss the mechanisms that link autophagy to tumour suppression and promotion and provide examples of the dual functions of autophagy in cancer...
  70. doi request reprint Dual degradation mechanisms ensure disposal of NHE6 mutant protein associated with neurological disease
    Ingrid Roxrud
    Centre for Cancer Biomedicine, University of Oslo, Montebello, N 0310 Oslo, Norway
    Exp Cell Res 315:3014-27. 2009
    ..Our results suggest that NHE6 participates in regulation of endosomal pH and provides a cellular basis for understanding the loss of NHE6 function leading to a neurological phenotype resembling Angelman syndrome...
  71. doi request reprint p62, an autophagy hero or culprit?
    Tor Erik Rusten
    Centre for Cancer Biomedicine, University of Oslo and Department of Biochemistry, The Norwegian Radium Hospital Montebello, N 0310, Oslo, Norway
    Nat Cell Biol 12:207-9. 2010
    ..Lack of autophagy leads to accumulation of p62, which is not good for liver cells, as it induces a cellular stress response that leads to disease...
  72. pmc Autophagic degradation of dBruce controls DNA fragmentation in nurse cells during late Drosophila melanogaster oogenesis
    Ioannis P Nezis
    Centre for Cancer Biomedicine, Institute for Cancer Research, The Norwegian Radium Hospital, Oslo University Hospital, Oslo, Norway
    J Cell Biol 190:523-31. 2010
    ..Our results reveal autophagic degradation of an IAP as a novel mechanism of triggering cell death and thereby provide a mechanistic link between autophagy and cell death...
  73. doi request reprint ESCRTing membrane deformation
    Lene Malerød
    Centre of Cancer Biomedicine, Faculty of Medicine, University of Oslo, Montebello, N 0310 Oslo, Norway
    Cell 136:15-7. 2009
    ..In this issue, Saksena et al. (2009) use an elegant fluorescence-based approach to define the sequential activation, recruitment, and disassembly of ESCRT-III subunits during membrane involution in vitro...
  74. ncbi request reprint The phosphatidylinositol 3-phosphate-binding FYVE finger
    Harald Stenmark
    Department of Biochemistry, The Norwegian Radium Hospital, Oslo, Norway
    FEBS Lett 513:77-84. 2002
    ..Here we review recent work that sheds light on the targeting of FYVE finger proteins to PI3P-containing membranes, and how these proteins serve to regulate multiple cellular functions...
  75. doi request reprint Ultrastructural characterization of giant endosomes induced by GTPase-deficient Rab5
    Catherine Sem Wegner
    Department of Biochemistry, Institute for Cancer Research, The Norwegian Radium Hospital, Oslo University Hospital, Montebello, Oslo, Norway
    Histochem Cell Biol 133:41-55. 2010
    ..In addition, we observe defects in the sorting of the EGF receptor and the transferrin receptor through this compartment...
  76. ncbi request reprint Aberrant receptor signaling and trafficking as mechanisms in oncogenesis
    Kaisa Haglund
    Centre for Cancer Biomedicine, University of Oslo, and Department of Biochemistry, Institute for Cancer Research, The Norwegian Radium Hospital, Montebello, 0310 Oslo, Norway
    Crit Rev Oncog 13:39-74. 2007
    ..In this review we will discuss these signaling pathways and how their dysfunction may contribute to oncogenesis. We will also highlight the important role of endocytic membrane trafficking in receptor signaling and tumor suppression...
  77. ncbi request reprint ESCRTs and Fab1 regulate distinct steps of autophagy
    Tor Erik Rusten
    Centre for Cancer Biomedicine, University of Oslo, N 0310 Oslo, Norway
    Curr Biol 17:1817-25. 2007
    ..Thus, this study links ESCRT function with autophagy and aggregate-induced neurodegeneration, thereby providing a plausible explanation for the fact that ESCRT mutations are involved in inherited neurodegenerative disease in humans [4]...
  78. ncbi request reprint ESCRTs
    Lene Malerød
    Department of Biochemistry, Institute for Cancer Research, Norwegian Radium Hospital, Montebello, N 0310 Oslo, Norway
    Curr Biol 17:R42-3. 2007
  79. pmc Cbl-dependent ubiquitination is required for progression of EGF receptors into clathrin-coated pits
    Espen Stang
    Institute of Pathology, University of Oslo, 0027 Oslo, Norway
    Mol Biol Cell 15:3591-604. 2004
    ....
  80. ncbi request reprint The small GTPase Rab22 interacts with EEA1 and controls endosomal membrane trafficking
    Maria Kauppi
    Department of Molecular Medicine, National Public Health Institute KTL, Biomedicum, PO Box 104, Fin 00251 Helsinki, Finland
    J Cell Sci 115:899-911. 2002
    ..These results suggest that Rab22a may regulate the dynamic interactions of endosomal compartments and it may be involved in the communication between the biosynthetic and early endocytic pathways...
  81. pmc p62/SQSTM1 forms protein aggregates degraded by autophagy and has a protective effect on huntingtin-induced cell death
    Geir Bjørkøy
    Biochemistry Department, Institute of Medical Biology, University of Tromsø, 9037 Tromsø, Norway
    J Cell Biol 171:603-14. 2005
    ..We suggest that p62 may, via LC3, be involved in linking polyubiquitinated protein aggregates to the autophagy machinery...
  82. ncbi request reprint Modulation of receptor recycling and degradation by the endosomal kinesin KIF16B
    Sebastian Hoepfner
    Max Planck Institute for Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, D 01307 Dresden, Germany
    Cell 121:437-50. 2005
    ..We propose that this mechanism could have important implications for signaling...
  83. pmc Misfolding diverts CFTR from recycling to degradation: quality control at early endosomes
    Manu Sharma
    Hospital for Sick Children, Program in Cell and Lung Biology, 555 University Ave, Toronto, Ontario M5G 1X8, Canada
    J Cell Biol 164:923-33. 2004
    ....
  84. ncbi request reprint The E3 ubiquitin ligase AIP4 mediates ubiquitination and sorting of the G protein-coupled receptor CXCR4
    Adriano Marchese
    Department of Microbiology and Immunology, Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, PA 19107, USA
    Dev Cell 5:709-22. 2003
    ..We propose a model whereby AIP4, Hrs, and Vps4 coordinate a cascade of ubiquitination and deubiquitination events that sort CXCR4 to the degradative pathway...
  85. ncbi request reprint Role of Rab5 in the recruitment of hVps34/p150 to the early endosome
    James T Murray
    Department of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, NY, USA
    Traffic 3:416-27. 2002
    ..However, Rab5 does not appear to act by directly recruiting p150/hVps34 complexes from the cytosol to the endosomal membrane...
  86. ncbi request reprint Regulation of ubiquitin-binding proteins by monoubiquitination
    Daniela Hoeller
    Institute for Biochemistry II, Goethe University Medical School, Theodor Stern Kai 7, 60590 Frankfurt, Germany
    Nat Cell Biol 8:163-9. 2006
    ..We propose that monoubiquitination of ubiquitin-binding proteins inhibits their capacity to bind to and control the functions of ubiquitinated targets in vivo...
  87. ncbi request reprint Double-sided ubiquitin binding of Hrs-UIM in endosomal protein sorting
    Satoshi Hirano
    Structural Biology Research Center, Photon Factory, Institute of Materials Structure Science, High Energy Accelerator Research Organization, Tsukuba, Ibaraki 305 0801, Japan
    Nat Struct Mol Biol 13:272-7. 2006
    ..Hrs-UIM belongs to a new subclass of double-sided UIMs, in contrast to its yeast homolog Vps27p, which has two tandem single-sided UIMs...
  88. pmc The mammalian phosphatidylinositol 3-phosphate 5-kinase (PIKfyve) regulates endosome-to-TGN retrograde transport
    Anna C Rutherford
    The Henry Wellcome Integrated Signalling Laboratories, Department of Biochemistry, School of Medical Sciences, University of Bristol, Bristol, BS8 1TD, UK
    J Cell Sci 119:3944-57. 2006
    ..It follows that the swollen endosomal phenotype observed in PIKfyve-suppressed cells results primarily from a reduction in retrograde membrane fission rather than a defect in multivesicular body biogenesis...
  89. ncbi request reprint Structural basis of ubiquitin recognition by mammalian Eap45 GLUE domain
    Satoshi Hirano
    Structural Biology Research Center, Photon Factory, Institute of Materials Structure Science, High Energy Accelerator Research Organization KEK, Tsukuba, Ibaraki 305 0801, Japan
    Nat Struct Mol Biol 13:1031-2. 2006
    ..In the Eap45 GLUE-Ub complex structure, Ub binds far from the proposed PI-binding site of Eap45 GLUE, suggesting their independent binding...
  90. ncbi request reprint Working out coupled monoubiquitination
    Kaisa Haglund
    Nat Cell Biol 8:1218-9. 2006
  91. pmc Endocytic pathways regulate Toll-like receptor 4 signaling and link innate and adaptive immunity
    Harald Husebye
    Institute of Cancer Research and Molecular Medicine, The Norwegian University of Science and Technology, Trondheim, Norway
    EMBO J 25:683-92. 2006
    ..Our results show that endosomal trafficking of the LPS receptor complex is essential for signal termination and LPS-associated antigen presentation, thus controlling both innate and adaptive immunity through TLR4...
  92. ncbi request reprint Early endosomal regulation of Smad-dependent signaling in endothelial cells
    Ekaterini Panopoulou
    Laboratory of Biological Chemistry, University of Ioannina Medical School, 45110 Ioannina, Greece
    J Biol Chem 277:18046-52. 2002
    ..The results suggest an important interconnection between early endosomal dynamics and TGFbeta/activin signal transduction pathways...
  93. pmc SLC9A6 mutations cause X-linked mental retardation, microcephaly, epilepsy, and ataxia, a phenotype mimicking Angelman syndrome
    Gregor D Gilfillan
    Department of Medical Genetics, Ulleval University Hospital, NO 0407 Oslo, Norway
    Am J Hum Genet 82:1003-10. 2008
    ..Therefore, mutations in SLC9A6 cause X-linked mental retardation. Additionally, males with findings suggestive of unexplained Angelman syndrome should be considered as potential candidates for SLC9A6 mutations...
  94. ncbi request reprint RILP is required for the proper morphology and function of late endosomes
    Cinzia Progida
    Dipartimento di Scienze e Tecnologie Biologiche ed Ambientali, Universita del Salento, Via Provinciale Monteroni, 73100 Lecce, Italy
    J Cell Sci 120:3729-37. 2007
    ..We propose that RILP might coordinate the biogenesis of MVEs with dynein-mediated motility...
  95. ncbi request reprint Endocytosis of the dermatan sulfate proteoglycan decorin utilizes multiple pathways and is modulated by epidermal growth factor receptor signaling
    David Denis Sofeu Feugaing
    Department of Obstetrics and Gynecology, Domagkstr 11, Munster University Hospital, D 48149 Munster, Germany
    Biochimie 89:637-57. 2007
    ..Of note, lipid-raft-dependent EGFR signaling modulates decorin uptake, suggesting the presence of a potential feedback regulation mechanism for desensitization of signaling events mediated by decorin...
  96. ncbi request reprint Developmental biology: moonlighting at the pole
    Tor Erik Rusten
    Nature 445:497-9. 2007
  97. ncbi request reprint The biogenesis of multivesicular endosomes
    Jean Gruenberg
    Department of Biochemistry, University of Geneva, 30 Quai Ernest Ansermet, 1211 Geneva 4, Switzerland
    Nat Rev Mol Cell Biol 5:317-23. 2004
  98. pmc Acquisition of Hrs, an essential component of phagosomal maturation, is impaired by mycobacteria
    Otilia V Vieira
    Cell Biology Program, Hospital for Sick Children, and Department of Biochemistry, University of Toronto, Ontario M5G 1X8, Canada
    Mol Cell Biol 24:4593-604. 2004
    ..These findings suggest that the inability to recruit Hrs contributes to the arrest of phagosomal maturation induced by pathogenic mycobacteria...
  99. ncbi request reprint The Rab5 effector Rabaptin-5 and its isoform Rabaptin-5delta differ in their ability to interact with the small GTPase Rab4
    Elena Korobko
    Institute of Gene Biology, Russian Academy of Sciences, Moscow, Russia
    FEBS J 272:37-46. 2005
    ..Our data suggests that while Rabaptin-5 was proposed to act as a molecular linker between Rab5 and Rab4, to coordinate endocytic and recycling traffic, Rabaptin-5delta is involved only in the Rab5-driven events...
  100. ncbi request reprint Actin-based motility of endosomes is linked to the polar tip growth of root hairs
    Boris Voigt
    Institute of Cellular and Molecular Botany, University of Bonn, Kirschallee 1, D 53115 Bonn, Germany
    Eur J Cell Biol 84:609-21. 2005
    ..In conclusion, actin-propelled endosomal motility is tightly linked to the polar tip growth of root hairs...
  101. pmc The ESCRT-III subunit hVps24 is required for degradation but not silencing of the epidermal growth factor receptor
    Kristi G Bache
    Department of Biochemistry, Institute for Cancer Research, Norwegian Radium Hospital, Montebello, N 0310 Oslo, Norway
    Mol Biol Cell 17:2513-23. 2006
    ..Together, our results reveal functional differences between ESCRT-I and ESCRT-III in degradative protein trafficking and indicate that degradation of the EGFR is not required for termination of its signaling...