M Sioud

Summary

Affiliation: The Norwegian Radium Hospital
Country: Norway

Publications

  1. ncbi request reprint Nucleic acid enzymes as a novel generation of anti-gene agents
    M Sioud
    Institute for Cancer Research, Department of Immunology, The Norwegian Radium Hospital, Montebello, Oslo
    Curr Mol Med 1:575-88. 2001
  2. ncbi request reprint Ribozymes, DNAzymes and small interfering RNAs as therapeutics
    Mouldy Sioud
    Institute for Cancer Research, Department of Immunology, Molecular Medicine Group, The Norwegian Radium Hospital, Montebello, 0310 Oslo, Norway
    Curr Drug Targets 6:647-53. 2005
  3. pmc How does autoimmunity cause tumor regression? A potential mechanism involving cross-reaction through epitope mimicry
    Mouldy Sioud
    The Norweigian Radium Hospital, Institute for Cancer Research, Department of Immunology, Molecular Medicine Group, Oslo, Norway
    Mol Med 8:115-9. 2002
  4. ncbi request reprint Single-stranded small interfering RNA are more immunostimulatory than their double-stranded counterparts: a central role for 2'-hydroxyl uridines in immune responses
    Mouldy Sioud
    The Norwegian Radium Hospital, Department of Immunology, Molecular Medicine Group Montebello, Oslo, Norway
    Eur J Immunol 36:1222-30. 2006
  5. ncbi request reprint Induction of inflammatory cytokines and interferon responses by double-stranded and single-stranded siRNAs is sequence-dependent and requires endosomal localization
    Mouldy Sioud
    The Norwegian Radium Hospital, Department of Immunology, Molecular Medicine Group, Montebello, N 0310 Oslo, Norway
    J Mol Biol 348:1079-90. 2005
  6. ncbi request reprint Generation of an effective anti-tumor immunity after immunization with xenogeneic antigens
    Mouldy Sioud
    Department of Immunology, Institute for Cancer Research, The Norwegian Radium Hospital, Oslo, Norway
    Eur J Immunol 33:38-45. 2003
  7. ncbi request reprint Does the understanding of immune activation by RNA predict the design of safe siRNAs?
    Mouldy Sioud
    Institute for Cancer Research, Department of Immunology, Molecular Medicine Group, Montebello, N 0310 Oslo, Norway
    Front Biosci 13:4379-92. 2008
  8. ncbi request reprint Transcriptional responses of Bacillus subtillis and thuringiensis to antibiotics and anti-tumour drugs
    Mouldy Sioud
    Department of Immunology, The Institute for Cancer Research, The Norwegian Radium Hospital, Montebello, N 0310 Oslo, Norway
    Int J Mol Med 23:33-9. 2009
  9. ncbi request reprint Profiling microRNA expression using sensitive cDNA probes and filter arrays
    Mouldy Sioud
    Department of Immunology, Institute for Cancer Research, The Norwegian Radium Hospital, Oslo, Norway
    Biotechniques 37:574-6, 578-80. 2004
  10. ncbi request reprint Innate sensing of self and non-self RNAs by Toll-like receptors
    Mouldy Sioud
    The Norwegian Radium Hospital, Department of Immunology, Molecular Medicine Group, Montebello N 0310 Oslo, Norway
    Trends Mol Med 12:167-76. 2006

Collaborators

Detail Information

Publications89

  1. ncbi request reprint Nucleic acid enzymes as a novel generation of anti-gene agents
    M Sioud
    Institute for Cancer Research, Department of Immunology, The Norwegian Radium Hospital, Montebello, Oslo
    Curr Mol Med 1:575-88. 2001
    ..Relevant in vivo problems of the technology, mRNA repair by group I intron ribozymes and gene regulation by endogenous RNA will also be discussed...
  2. ncbi request reprint Ribozymes, DNAzymes and small interfering RNAs as therapeutics
    Mouldy Sioud
    Institute for Cancer Research, Department of Immunology, Molecular Medicine Group, The Norwegian Radium Hospital, Montebello, 0310 Oslo, Norway
    Curr Drug Targets 6:647-53. 2005
    ..Their potential use in cell cultures and in animal models for disease is also highlighted...
  3. pmc How does autoimmunity cause tumor regression? A potential mechanism involving cross-reaction through epitope mimicry
    Mouldy Sioud
    The Norweigian Radium Hospital, Institute for Cancer Research, Department of Immunology, Molecular Medicine Group, Oslo, Norway
    Mol Med 8:115-9. 2002
    ..This strategy may down the friendly established relationship between tumor tissues and the cells of the immune system...
  4. ncbi request reprint Single-stranded small interfering RNA are more immunostimulatory than their double-stranded counterparts: a central role for 2'-hydroxyl uridines in immune responses
    Mouldy Sioud
    The Norwegian Radium Hospital, Department of Immunology, Molecular Medicine Group Montebello, Oslo, Norway
    Eur J Immunol 36:1222-30. 2006
    ..Collectively, the data should facilitate the development of siRNA therapeutics and expand the understanding of how RNA is sensed by innate immunity...
  5. ncbi request reprint Induction of inflammatory cytokines and interferon responses by double-stranded and single-stranded siRNAs is sequence-dependent and requires endosomal localization
    Mouldy Sioud
    The Norwegian Radium Hospital, Department of Immunology, Molecular Medicine Group, Montebello, N 0310 Oslo, Norway
    J Mol Biol 348:1079-90. 2005
    ....
  6. ncbi request reprint Generation of an effective anti-tumor immunity after immunization with xenogeneic antigens
    Mouldy Sioud
    Department of Immunology, Institute for Cancer Research, The Norwegian Radium Hospital, Oslo, Norway
    Eur J Immunol 33:38-45. 2003
    ..Taken together, the data establish the in vivo applicability of the cross-stimulation between self and foreign antigens as an alternative way to break tolerance against the poorly immunogenic gliomas...
  7. ncbi request reprint Does the understanding of immune activation by RNA predict the design of safe siRNAs?
    Mouldy Sioud
    Institute for Cancer Research, Department of Immunology, Molecular Medicine Group, Montebello, N 0310 Oslo, Norway
    Front Biosci 13:4379-92. 2008
    ....
  8. ncbi request reprint Transcriptional responses of Bacillus subtillis and thuringiensis to antibiotics and anti-tumour drugs
    Mouldy Sioud
    Department of Immunology, The Institute for Cancer Research, The Norwegian Radium Hospital, Montebello, N 0310 Oslo, Norway
    Int J Mol Med 23:33-9. 2009
    ..Collectively, the present study would assist in defining common and/or different pathways affected by eubacterial and eukaryotic type DNA topoisomerase inhibitors in the same living organism...
  9. ncbi request reprint Profiling microRNA expression using sensitive cDNA probes and filter arrays
    Mouldy Sioud
    Department of Immunology, Institute for Cancer Research, The Norwegian Radium Hospital, Oslo, Norway
    Biotechniques 37:574-6, 578-80. 2004
    ..The present study should facilitate a high-throughput analysis of miRNA expression between samples...
  10. ncbi request reprint Innate sensing of self and non-self RNAs by Toll-like receptors
    Mouldy Sioud
    The Norwegian Radium Hospital, Department of Immunology, Molecular Medicine Group, Montebello N 0310 Oslo, Norway
    Trends Mol Med 12:167-76. 2006
    ..Here, we discuss the recent developments that have expanded the understanding of self-non-self discrimination of RNAs by the innate immune system, and consider future directions for therapeutic applications of these findings...
  11. ncbi request reprint Therapeutic siRNAs
    Mouldy Sioud
    The Norwegian Radium Hospital, Department of Immunology, Molecular Medicine Group, Montebello, N 310, Oslo, Norway
    Trends Pharmacol Sci 25:22-8. 2004
    ..Beyond their value for dissecting gene functions and target validation, siRNAs also hold great potential as gene-specific therapeutic agents...
  12. ncbi request reprint On the delivery of small interfering RNAs into mammalian cells
    Mouldy Sioud
    The Norwegian Radium Hospital, Department of Immunology, Molecular Medicine Group, Montebello, Oslo
    Expert Opin Drug Deliv 2:639-51. 2005
    ..This review highlights recent advances in siRNA delivery, as well as challenging problems related to immune stimulation...
  13. ncbi request reprint TLR agonists induce the differentiation of human bone marrow CD34+ progenitors into CD11c+ CD80/86+ DC capable of inducing a Th1-type response
    Mouldy Sioud
    Department of Immunology, Institute for Cancer Research, Rikshospitalet Radiumhospitalet Medical Center, Oslo, Norway
    Eur J Immunol 37:2834-46. 2007
    ..Collectively, these data highlight the differential effects of TLR agonists on human bone marow CD34(+) progenitor cells and provide a new opportunity for generating functional DC that would be useful in cancer vaccination...
  14. ncbi request reprint Suppression of immunostimulatory siRNA-driven innate immune activation by 2'-modified RNAs
    Mouldy Sioud
    Department of Immunology, Institute for Cancer Research, Rikshopitalet Radiumhospitalet Medical Center, Oslo, Norway
    Biochem Biophys Res Commun 361:122-6. 2007
    ..Therefore, this new generation of TLR antagonists can be used as immunosuppressive agents to interfere with TLR signaling...
  15. ncbi request reprint RNA interference and innate immunity
    Mouldy Sioud
    Institute for Cancer Research, Department of Immunology, Molecular Medicine Group, Montebello, N 0310 Oslo, Norway
    Adv Drug Deliv Rev 59:153-63. 2007
    ..Also, it highlights the spectrum of molecular strategies allowing the design of therapeutic siRNAs with minimal side effects...
  16. ncbi request reprint Signaling through toll-like receptor 7/8 induces the differentiation of human bone marrow CD34+ progenitor cells along the myeloid lineage
    Mouldy Sioud
    Department of Immunology, Radiumhospitalet University Hospital, Oslo, Montebello N 0310, Norway
    J Mol Biol 364:945-54. 2006
    ..Thus, TLR signaling may regulate BM cell development in humans...
  17. doi request reprint Recent advances in small interfering RNA sensing by the immune system
    Mouldy Sioud
    Institute for Cancer Research, Department of Immunology, Molecular Medicine Group, Montebello, Oslo, Norway
    N Biotechnol 27:236-42. 2010
    ..This minireview summarizes our current knowledge of siRNA sensing by the immune receptors and how to separate siRNA unwanted effects from gene silencing...
  18. ncbi request reprint Profiling the immune response in patients with breast cancer by phage-displayed cDNA libraries
    M Sioud
    Institute for Cancer Research, Department of Immunology, Molecular Medicine Group The Norwegian Radium Hospital, Oslo, Norway
    Eur J Immunol 31:716-25. 2001
    ..The obtained results demonstrate that phage display could be a valuable method for the identification of antigens recognized by the humoral immune system in patients with cancer...
  19. doi request reprint Promises and challenges in developing RNAi as a research tool and therapy
    Mouldy Sioud
    Molecular Medicine Group, Department of Immunology, Institute for Cancer Research, The Norwegian Radium Hospital, Montebello, Oslo, Norway
    Methods Mol Biol 703:173-87. 2011
    ..This chapter summarizes recent progress in siRNA design, sensing by the immune system, and discusses some of the promising approaches that are currently being explored in separating siRNA unwanted effects from gene silencing...
  20. ncbi request reprint Protein kinase C-alpha isoform is involved in erythropoietin-induced erythroid differentiation of CD34(+) progenitor cells from human bone marrow
    J H Myklebust
    Department of Immunology, The Norwegian Radium Hospital, Oslo, Norway
    Blood 95:510-8. 2000
    ..Taken together, these findings point to a novel role for the PKC-alpha isoform in mediating EPO-induced erythroid differentiation of the CD34(+) progenitor cells from human bone marrow. (Blood. 2000;95:510-518)..
  21. ncbi request reprint Identification of immunogenic antigens using a phage-displayed cDNA library from an invasive ductal breast carcinoma tumour
    M H Hansen
    Department of Immunology, Molecular Medicine Group, The Norwegian Radium Hospital, Oslo, Norway
    Int J Oncol 19:1303-9. 2001
    ..Both the known and unknown immunoselected gene products should offer an additional source for cancer gene discovery for diagnostic testing and vaccine development...
  22. pmc Antigen-specific IgG antibodies in stage IV long-time survival breast cancer patients
    M H Hansen
    Department of Immunology, Molecular Medicine, Norwegian Radium Hospital, Oslo
    Mol Med 7:230-9. 2001
    ..Thus, the identified B-cell epitopes should be of great interest in vaccine development...
  23. doi request reprint Evidence for the involvement of galectin-3 in mesenchymal stem cell suppression of allogeneic T-cell proliferation
    M Sioud
    Department of Immunology, The Institute for Cancer Research, Rikshospitalet Radiumhospitalet Medical Center, Oslo, Norway
    Scand J Immunol 71:267-74. 2010
    ..05). Collectively, the data emphasize a new role of galectin-3 in the immunomodulatory function of MSC and indicate that NOD signalling pathway is also functional in these cells...
  24. ncbi request reprint A nuclease-resistant protein kinase C alpha ribozyme blocks glioma cell growth
    M Sioud
    Department of Immunology, Norwegian Radium Hospital, Montebello, Oslo, Norway
    Nat Biotechnol 16:556-61. 1998
    ..A single injection of cationic liposome ribozyme complexes into glioma tumors inhibited tumor growth, demonstrating both the efficacy of the ribozyme and a major role of PKC alpha in tumor growth...
  25. doi request reprint Does our current understanding of immune tolerance, autoimmunity, and immunosuppressive mechanisms facilitate the design of efficient cancer vaccines?
    M Sioud
    Department of Immunology, The Norwegian Radium Hospital, Institute for Cancer Research, Oslo, Norway
    Scand J Immunol 70:516-25. 2009
    ..This review highlights the problem of immune tolerance, the induction of autoreactive T cells, and describes strategies to enhance tumour immunity...
  26. ncbi request reprint Differential expression of bcl-2 homologs in human CD34(+) hematopoietic progenitor cells induced to differentiate into erythroid or granulocytic cells
    D Josefsen
    Department of Immunology, Institute for Cancer Research, The Norwegian Radium Hospital, Montebello, Oslo, Norway
    Stem Cells 18:261-72. 2000
    ..The use of Bcl-x(L)-specific ribozymes suggested that Bcl-x(L) is important for survival but not for differentiation of erythroid progenitor cells...
  27. ncbi request reprint Therapeutic RNA and DNA enzymes
    M Sioud
    Institute for Cancer Research, Department of Immunology, The Norwegian Radium Hospital, Montebello, N 0310 Oslo, Norway
    Biochem Pharmacol 60:1023-6. 2000
    ..Furthermore, such agents can be a valuable tool for probing gene function...
  28. pmc Ribozyme inhibition of the protein kinase C alpha triggers apoptosis in glioma cells
    M Leirdal
    Institute for Cancer Research, Department of Immunology, The Norwegian Radium Hospital, Oslo
    Br J Cancer 80:1558-64. 1999
    ..PKC alpha ribozymes may prove useful in the management of malignant gliomas...
  29. ncbi request reprint Increased miR-21 expression during human monocyte differentiation into DCs
    Lina Cekaite
    Department of Immunology, Faculty Division, Oslo University Hospital, Oslo, Norway
    Front Biosci (Elite Ed) 2:818-28. 2010
    ..Collectively, the present study highlights the involvement of miRNAs, particularly miR-21 in monocyte differentiation to DCs and identifies potential miR-21 target genes...
  30. ncbi request reprint High-throughput analysis of microRNA gene expression using sensitive probes
    Mouldy Sioud
    Molecular Medicine Group, Institute for Cancer Research, Oslo, Norway
    Methods Mol Biol 309:311-20. 2005
  31. ncbi request reprint Cationic liposome-mediated delivery of siRNAs in adult mice
    Mouldy Sioud
    Department of Immunology, Molecular Medicine Group, The Norwegian Radium Hospital, 0310, Montebello, Norway
    Biochem Biophys Res Commun 312:1220-5. 2003
    ..Taken together, the data provide a basis for lipid-mediated systemic delivery of siRNAs and indicate that certain siRNA sequences can activate the innate immunity response genes that can be beneficial for the treatment of cancer...
  32. ncbi request reprint Targeting Raf-1 gene expression by a DNA enzyme inhibits juvenile myelomonocytic leukemia cell growth
    Per Ole Iversen
    Institute for Nutrition Research, University of Oslo, and Department of Immunology, Molecular Medicine Group, The Norwegian Radium Hospital, Oslo, Norway
    Blood 99:4147-53. 2002
    ..The DNA enzyme efficiently catabolized mRNA-Raf-1 with subsequent inhibition of JMML cell growth, suggesting its potential as a mechanism-based therapy in this fatal leukemia...
  33. doi request reprint NOD2/CARD15 on bone marrow CD34+ hematopoietic cells mediates induction of cytokines and cell differentiation
    Mouldy Sioud
    Institute for Cancer Research, The Norwegian Radium Hospital, Montebello, HN 0310, Oslo, Norway
    J Leukoc Biol 85:939-46. 2009
    ..Although the best-known function of NLRs involves mature cells, our data highlight for the first time the functionality of these receptors in human BM CD34+ hematopoietic cells...
  34. pmc Impaired expression of indoleamine 2, 3-dioxygenase in monocyte-derived dendritic cells in response to Toll-like receptor-7/8 ligands
    Gro Furset
    Rikshospitalet Radiumhospitalet Medical Center, Institute for Cancer Research, Department of Immunology, Molecular Medicine Group, Montebello, Oslo, Norway
    Immunology 123:263-71. 2008
    ..The findings that 2'-O-methyl modified RNAs can block isRNA-induced IDO expression would facilitate the design of new TLR inhibitors...
  35. ncbi request reprint Selective targeting of cancer cells using synthetic peptides
    Mohsen Shadidi
    Department of Immunology, Molecular Medicine Group, The Norwegian Radium Hospital, Institute for Cancer Research, Montebello, N0310 Oslo, Norway
    Drug Resist Updat 6:363-71. 2003
    ..Here we give an update on the progress made during the last two years on the identification and potential of specific synthetic tumor targeting peptides...
  36. ncbi request reprint Gene-array analysis of glioma cells after treatment with an anti-PKCalpha siRNA: a general protocol
    Marianne Leirdal
    Department of Immunology, Molecular Medicine Group, The Norwegian Radium Hospital, Oslo
    Methods Mol Biol 252:493-500. 2004
    ..The data indicate that siRNAs are an ideal tool for target validation, and show that RNA-dependent RNA polymerase is not required for RNAi in mammalian cells...
  37. ncbi request reprint Gene expression analysis in blood cells in response to unmodified and 2'-modified siRNAs reveals TLR-dependent and independent effects
    Lina Cekaite
    Department of Tumour Biology, Institute for Cancer Research, The Norwegian Radium Hospital, University of Oslo, Montebello, 310, Oslo, Norway
    J Mol Biol 365:90-108. 2007
    ..The data should help to define strategies to either enhance or avoid the non-specific effects of siRNAs in order to develop safe therapeutics...
  38. pmc Limited heterogeneity in the T-cell receptor V-gene usage in lymphocytes infiltrating human colorectal tumours
    B Ostenstad
    Institute of Immunology and Rheumatology, National Hospital, Oslo, Norway
    Br J Cancer 69:1078-82. 1994
    ..Our results demonstrate a limited heterogeneity in the V-gene usage of TILs from seven patients with colorectal cancers, suggesting a local antigen-driven immune response at the tumour site...
  39. ncbi request reprint Substitution of the 2'-hydroxyl group at position 2.1 by an amino group interferes with Mg(2+) binding and efficient cleavage by hammerhead ribozyme
    M Sioud
    Department of Immunology, The Norwegian Radium Hospital, Oslo, Montebello, N 0310, Norway
    Biochem Biophys Res Commun 262:461-6. 1999
    ..Taken together, our findings indicate that changes in the sugar at position 2.1 alter Mg(2+)-promoting ribozyme cleavage...
  40. doi request reprint Profiling of miRNA expression and prediction of target genes
    Mouldy Sioud
    Department of Immunology, Institute for Cancer Research, Radiumhospitalet Rickshospitalet University Hospital, Oslo, Norway
    Methods Mol Biol 629:257-71. 2010
    ..This chapter highlights the technical advances in miRNA gene profiling and discusses the limitations of target prediction programs and the necessity of defining the roles that govern miRNA specificity and target discrimination in vivo...
  41. doi request reprint Optimized protocols for siRNA delivery into monocytes and dendritic cells
    Anne Mobergslien
    Departments of Immunology, Institute for Cancer Research, Radiumhospitalet Rikshopitalet University Hospital, Oslo, Norway
    Methods Mol Biol 629:71-85. 2010
    ..This chapter describes detailed protocols for introducing siRNAs into human monocytes and dendritic cells using standard electroporation techniques...
  42. doi request reprint Development of TLR7/8 small RNA antagonists
    Mouldy Sioud
    Department of Immunology, Institute for Cancer Research, Rikshospitalet Radiumhospitalet, University Hospital, Oslo, Norway
    Methods Mol Biol 629:387-94. 2010
    ..This chapter describes the characterization of immunosuppressive RNA oligonucleotides carrying 2'-modified uridines...
  43. doi request reprint Modulation of dendritic cell maturation and function by siRNA-bearing 5'-triphosphate
    Mouldy Sioud
    Department of Immunology, Institute for Cancer Research, Rikshospitalet Radiumhospitalet, University Hospital, Oslo, Norway
    Methods Mol Biol 629:395-404. 2010
    ..This chapter describes the in vitro synthesis of siRNA targeting indoleamine 2,3-dioxygenase (IDO), an enzyme that plays a critical role in hindering immunity against tumours...
  44. pmc Modulation of dendritic cell maturation and function with mono- and bifunctional small interfering RNAs targeting indoleamine 2,3-dioxygenase
    Gro F Flatekval
    Department of Immunology, Institute for Cancer Research, Rikshospitalet Radiumhospitalet Medical Centre, Montebello, Oslo, Norway
    Immunology 128:e837-48. 2009
    ..Collectively, our data highlight the potential therapeutic applications of this new generation of siRNAs in immunotherapy...
  45. doi request reprint Expression profiling of microRNAs in cancer cells: technical considerations
    Mouldy Sioud
    Department of Immunology, Institute for Cancer Research, Rikshopitalet Radiumhospitalet Medical Center, Montebello, Oslo, Norway
    Methods Mol Biol 439:179-90. 2008
    ..This chapter summarizes these recent findings and highlights the technical advances in miRNA probe preparation, miRNA expression profiling and target identification...
  46. ncbi request reprint Transcriptional targeting of small interfering RNAs into cancer cells
    Trang Huynh
    Department of Immunology, Molecular Medicine Group, University Department, University of Oslo, The Norwegian Radium Hospital, Montebello, N 310 Oslo, Norway
    Biochem Biophys Res Commun 350:854-9. 2006
    ..Collectively, these data indicate that the survivin promoter is suitable for directing siRNA expression in cancer cells, but not normal cells...
  47. ncbi request reprint An overview of the immune system and technical advances in tumor antigen discovery and validation
    Mouldy Sioud
    Department of Immunology, Institute for Cancer Research, The Norwegian Radium Hospital, University of Oslo, Oslo, Norway
    Methods Mol Biol 360:277-318. 2007
    ..It also outlines the technical advances in tumor antigen discovery and validation...
  48. ncbi request reprint Gene silencing in mammalian cells by preformed small RNA duplexes
    Marianne Leirdal
    Department of Immunology, Molecular Medicine Group, The Norwegian Radium Hospital, Montebello, Oslo N 0310, Norway
    Biochem Biophys Res Commun 295:744-8. 2002
    ..Taken together, the present data indicate that in vitro transcribed siRNA can be useful for silencing gene expression. Additionally, bi- and perhaps poly-siRNAs may be expressed and processed in mammalian cells...
  49. pmc Combination of endostatin and a protein kinase Calpha DNA enzyme improves the survival of rats with malignant glioma
    Dag R Sorensen
    Department of Comparative Medicine, The National Hospital, University of Oslo, Oslo, Norway
    Neoplasia 4:474-9. 2002
    ..0009) or endostatin (P<.025) alone. Thus, the combined treatment may represent an attractive therapeutic strategy against malignant gliomas...
  50. ncbi request reprint Strategies for the design of random siRNA libraries and the selection of anti-GFP siRNAs
    Mouldy Sioud
    Molecular Medicine Group, Institute for Cancer Research, Oslon, Norway
    Methods Mol Biol 309:83-91. 2005
  51. doi request reprint A combined immunostimulatory and immunoinhibitory short interference RNA reduces hypercoagulability in a rat model of acute promyelocytic leukaemia
    Per Ole Iversen
    Department of Nutrition, Faculty of Medicine, Institute of Basic Medical Sciences, University of Oslo, Oslo, Norway
    Thromb Haemost 104:350-4. 2010
    ..05) upon treatment with the siRNA. The bifunctional siRNA reduces the hypercoagulable state in APL in addition to its direct anti-leukaemic properties, supporting testing of this small molecule in human APL...
  52. ncbi request reprint Druggable signaling proteins
    Mouldy Sioud
    Department of Immunology, Institute for Cancer Research, The Norwegian Radium Hospital, University of Oslo, Oslo, Norway
    Methods Mol Biol 361:1-24. 2007
    ..These include imatinib (anti-BCR-ABL), gefitinib (anti-EGF receptor), and herpetin (anti-ErbB-2). Here, some selected validated and drugable targets are summarized...
  53. doi request reprint New insights into mesenchymal stromal cell-mediated T-cell suppression through galectins
    M Sioud
    Department of Immunology, Institute for Cancer Research, Radiumhospitalet Rikshospitalet University Hospital, Montebello, Oslo, Norway
    Scand J Immunol 73:79-84. 2011
    ....
  54. ncbi request reprint Probing the specificity of human myeloma proteins with a random peptide phage library
    A Dybwad
    Department of Immunology, Molecular Medicine Group, The Norwegian Radium Hospital, Montebello, Norway
    Scand J Immunol 57:583-90. 2003
    ..g. neural cell adhesion proteins) and pathogen-derived proteins (e.g. herpes simplex virus capsid proteins). The selected peptides could be useful as targeting agents for myeloma cells expressing surface immunoglobulins...
  55. ncbi request reprint Systemic delivery of synthetic siRNAs
    Mouldy Sioud
    Department of Immunology, Molecular Medicine Group, The Norwegian Radium Hospital, Oslo
    Methods Mol Biol 252:515-22. 2004
    ..However, the major obstacle to the use of siRNAs as therapeutics is the difficulty involved in effective in vivo delivery. This chapter describes the liposomal delivery of siRNAs into adult mice...
  56. ncbi request reprint Ribozyme- and siRNA-mediated mRNA degradation: a general introduction
    Mouldy Sioud
    Department of Immunology, The Norwegian Radium Hospital, Oslo
    Methods Mol Biol 252:1-8. 2004
  57. ncbi request reprint Vector-based delivery of siRNAs: in vitro and in vivo challenges
    Sebastien Walchli
    Institute for Cancer Research, Department of Immunology, Molecular Medicine Group, Rikshospitalet Radiumhospitalet Medical Centre, Montebello 0310 Oslo, Norway
    Front Biosci 13:3488-93. 2008
    ..Here we review the recent advances in developing controllable expression vectors in order to accelerate the therapeutic applications of RNAi...
  58. ncbi request reprint siRNA delivery in vivo
    Mouldy Sioud
    Molecular Medicine Group, Institute for Cancer Research, Oslo, Norway
    Methods Mol Biol 309:237-49. 2005
  59. ncbi request reprint Mucosal vaccination with phage-displayed tumour antigens identified through proteomics-based strategy inhibits the growth and metastasis of 4T1 breast adenocarcinoma
    Mohsen Shadidi
    Department of Immunology, Molecular Medicine Group, Institute for Cancer Research, Rikshospitalet Radiumhospitalet Medical Center, Montebello, N 0310 Oslo, Norway
    Int J Oncol 32:241-7. 2008
    ..In addition, arrayed phage-displayed tumour antigens could be useful as a serum-based screening test for the detection of several tumour antigens...
  60. doi request reprint Targeted delivery of antisense oligonucleotides and siRNAs into mammalian cells
    Mouldy Sioud
    Department of Immunology, Institute for Cancer Research, The Norwegian Radium Hospital, University of Oslo, Norway
    Methods Mol Biol 487:61-82. 2009
    ..Also, transcriptional targeting strategies and detailed protocols for the selection of cancer cell-binding peptide from random peptide libraries are described...
  61. doi request reprint Advances in RNA sensing by the immune system: separation of siRNA unwanted effects from RNA interference
    Mouldy Sioud
    Department of Immunology, Institute for Cancer Research, Radiumhospitalet Rikshopitalet University Hospital, Oslo, Norway
    Methods Mol Biol 629:33-52. 2010
    ..This chapter describes how to separate gene silencing from immunostimulation. Also, it discusses the impact of these findings on the design of effective cancer vaccines...
  62. ncbi request reprint Design of bifunctional siRNAs: combining immunostimulation and gene-silencing in one single siRNA molecule
    Gro Furset
    Department of Immunology, Molecular Medicine Group, The Norwegian Radium Hospital, University of Oslo, Montebello, N 0310 Oslo, Norway
    Biochem Biophys Res Commun 352:642-9. 2007
    ..Interestingly, IL10 siRNA-transfected iMoDCs were capable for enhancing the response of allogeneic T cells, providing support for the rational design of bifunctional siRNAs as immune modulating therapy...
  63. ncbi request reprint Identification of novel carrier peptides for the specific delivery of therapeutics into cancer cells
    Mohsen Shadidi
    Institute for Cancer Research, Department of Immunology, Molecular Medicine Group, The Norwegian Radium Hospital, Montebello, N 0310 Oslo, Norway
    FASEB J 17:256-8. 2003
    ..Thus, efficient delivery of antisense oligonucleotides can be achieved by coupling them to cancer cell-specific peptides, identified by a method that did not require any knowledge about their corresponding receptors...
  64. ncbi request reprint Protein arrays: a versatile toolbox for target identification and monitoring of patient immune responses
    Lina Cekaite
    Department of Tumor Biology, Institute for Cancer Research, The Norwegian Radium Hospital, University of Oslo, Oslo, Norway
    Methods Mol Biol 360:335-48. 2007
    ..Here, we review the advantage of protein and serum screening of peptides and cDNA repertoires displayed on phages as well as the fabrication of protein microarrays for probing immune responses in patients...
  65. ncbi request reprint Analysis of the humoral immune response to immunoselected phage-displayed peptides by a microarray-based method
    Lina Cekaite
    Department of Tumor Biology, Institute for Cancer Research, The Norwegian Radium Hospital, Oslo, Norway
    Proteomics 4:2572-82. 2004
    ..A significant tumor effect was found with most of the selected phage-displayed peptides, suggesting that recombinant phage microarrays can serve as a tool in monitoring humoral responses towards phage-displayed peptides...
  66. ncbi request reprint Systematic identification of sense-antisense transcripts in mammalian cells
    Øystein Røsok
    The Norwegian Radium Hospital, Institute for Cancer Research, Department of Immunology, Molecular Medicine Group, Montebello, N 0310, Oslo, Norway
    Nat Biotechnol 22:104-8. 2004
    ..When the antisense RNAs were specifically targeted, their corresponding mRNA levels significantly altered, a result consistent with a regulatory role for the identified antisense RNAs...
  67. doi request reprint Overcoming self-tolerance to tumour cells
    Mouldy Sioud
    Department of Immunology, Institute for Cancer Research, Rikshospitalet Radiumhospitalet, University Hospital, Oslo, Norway
    Methods Mol Biol 629:495-505. 2010
    ..This review highlights some of the strategies used to break self-tolerance against solid and blood tumour cells. Also, the possibility of reprogramming DC and/or lymphocyte functions using small interfering RNAi (siRNA) is discussed...
  68. pmc Evidence for the involvement of Gi2 in activation of extracellular signal-regulated kinases in hepatocytes
    O Melien
    Department of Pharmacology, Faculty of Medicine, University of Oslo, Box 1057 Blindern, N 0316, Oslo, Norway
    BMC Cell Biol 2:13. 2001
    ..Activation of the extracellular signal-regulated kinases ERK1 and ERK2 in hepatocytes by prostaglandin (PG)F2alpha was recently found to be inhibited by pertussis toxin (PTX) suggesting a role for Gi proteins...
  69. doi request reprint What are the key targeted delivery technologies of siRNA now?
    Mouldy Sioud
    Department of Immunology, Institute for Cancer Research, Radiumhospitalet Rikshopitalet Universtity Hospital, Oslo, Norway
    Methods Mol Biol 629:93-107. 2010
    ..The present chapter discusses the current progress made in the specific delivery of siRNAs...
  70. doi request reprint Deciphering the code of innate immunity recognition of siRNAs
    Mouldy Sioud
    Department of Immunology, Institute for Cancer Research, The Norwegian Radium Hospital, University of Oslo, Norway
    Methods Mol Biol 487:41-59. 2009
    ....
  71. ncbi request reprint Identification of genes differentially expressed in breast cancer cell line SKBR3: potential identification of new prognostic biomarkers
    Marianne Leirdal
    Institute for Cancer Research, Department of Immunology, Molecular Medicine Group, The Norwegian Radium Hospital, Montebello, N 0310 Oslo, Norway
    Int J Mol Med 14:217-22. 2004
    ....
  72. doi request reprint Recent advances in hematopoietic stem cell transplantation and perspectives of RNAi applications
    Yngvar Fløisand
    Department of Hematology, Rikshopitalet Radiumhospitalet, University Hospital, Oslo, Norway
    Methods Mol Biol 629:507-23. 2010
    ..This chapter reviews the recent advances in ASCT and discusses the potential application of RNAi in hematopoietic cells...
  73. ncbi request reprint Identification of a novel centrosome/microtubule-associated coiled-coil protein involved in cell-cycle progression and spindle organization
    Sebastian Patzke
    Department of Immunology, The Norwegian Radium Hospital, Montebello, 0310 Oslo, Norway
    Oncogene 24:1159-73. 2005
    ..Taken together, we demonstrate that CSPP is associated with centrosomes and microtubules and may play a role in the regulation of G(1)/S-phase progression and spindle assembly...
  74. ncbi request reprint Systematic search for natural antisense transcripts in eukaryotes (review)
    Oystein Røsok
    Institute for Cancer Research, Molecular Medicine Group, Montebello, N 0310 Oslo, Norway
    Int J Mol Med 15:197-203. 2005
    ..This experimental strategy allows systematic analysis of sense and antisense partners in any cell or tissue type...
  75. ncbi request reprint Influence of light fluence rate on the effects of photodynamic therapy in an orthotopic rat glioma model
    Even Angell-Petersen
    Department of Surgical Oncology, The Norwegian Radium Hospital, Oslo, Norway
    J Neurosurg 104:109-17. 2006
    ..The effect depends on the total light energy delivered to the target tissue, but may also be influenced by the rate of light delivery...
  76. ncbi request reprint Discovery of differentially expressed genes: technical considerations
    Øystein Røsok
    Department of Immunology, Institute for Cancer Research, The Norwegian Radium Hospital, University of Oslo, Oslo, Norway
    Methods Mol Biol 360:115-29. 2007
    ..It should facilitate the discovery of differentially expressed genes...
  77. doi request reprint Evidence for the involvement of FAM110C protein in cell spreading and migration
    Helena Hauge
    Department of Immunology the Norwegian Radium Hospital Rikshospitalet University Hospital, Oslo, Norway
    Cell Signal 21:1866-73. 2009
    ..Collectively, these findings suggest a new function for FAM110C in the regulation of cell spreading, migration and filopodia induction...
  78. doi request reprint Systemic delivery of synthetic siRNAs
    Dag R Sørensen
    Department of Comparative Medicine, Rikshospitalet Radiumhospitalet, University Hospital, Oslo, Norway
    Methods Mol Biol 629:87-91. 2010
    ..This chapter describes cationic lipid delivery of siRNAs to adult mice...
  79. ncbi request reprint Gene silencing by systemic delivery of synthetic siRNAs in adult mice
    Dag R Sørensen
    Department of Comparative Medicine, The National Hospital, Oslo 0310, Norway
    J Mol Biol 327:761-6. 2003
    ..Collectively, these results demonstrate that synthetic siRNAs can function in vivo as pharmaceutical drugs...
  80. ncbi request reprint Inhibition of gene expression by nucleic acid enzymes in rodent models of human disease
    Per Ole Iversen
    Institute for Nutrition, University of Oslo, Norway
    Methods Mol Biol 252:451-6. 2004
    ..The described protocols should facilitate the in vivo evaluation of other oligonucleotide-based therapy such as small interfering RNAs (siRNAs)...
  81. ncbi request reprint Colon cancer cell-derived tumor necrosis factor-alpha mediates the tumor growth-promoting response in macrophages by up-regulating the colony-stimulating factor-1 pathway
    Karin Zins
    Laboratory for Cardiovascular Research, Department of Anatomy and Cell Biology, Vienna Medical University, Waehringerstrasse 13, A 1090 Vienna, Austria
    Cancer Res 67:1038-45. 2007
    ..Thus, interrupting tumor cell-macrophage communication by targeting TNF-alpha may provide an alternative therapeutic approach for the treatment of colon cancer...
  82. ncbi request reprint RNA interference below the immune radar
    Mouldy Sioud
    Nat Biotechnol 24:521-2. 2006
  83. ncbi request reprint Colony-stimulating factor-1 blockade by antisense oligonucleotides and small interfering RNAs suppresses growth of human mammary tumor xenografts in mice
    Seyedhossein Aharinejad
    Laboratory for Cardiovascular Research, Department of Anatomy and Cell Biology, Vienna Medical University, Waehringerstrasse 13, A 1090 Vienna, Austria
    Cancer Res 64:5378-84. 2004
    ..In addition, mouse survival significantly increased after CSF-1 blockade. These studies demonstrate that CSF-1 and CSF-1 receptor are potential therapeutic targets for the treatment of mammary cancer...
  84. ncbi request reprint Target validation using RNA interference in solid tumors
    Seyedhossein Aharinejad
    Laboratory for Cardiovascular Research, Center for Anatomy and Cell Biology, Vienna Medical University, Vienna, Austria
    Methods Mol Biol 361:227-38. 2007
    ..The suppression of CSF-1 by RNA interference is therefore a powerful tool to block gene function and influence tumor-stroma interactions in solid tumor development...
  85. ncbi request reprint Host CD147 blockade by small interfering RNAs suppresses growth of human colon cancer xenografts
    Dietmar Abraham
    Laboratory for Cardiovascular Research, Center of Anatomy and Cell Biology, Medical University of Vienna, Waehringerstrasse 13, A 1090 Vienna, Austria
    Front Biosci 13:5571-9. 2008
    ..Interestingly, inhibition of cancer cell-derived EMMPRIN leads to increased MMP-9 levels in vivo. Our findings provide new insights into CD147-mediated tumor-host interactions mediating colon cancer growth...
  86. ncbi request reprint Selection of peptides for specific delivery of oligonucleotides into cancer cells
    Mohsen Shadidi
    Department of Immunology, Molecular Medicine Group, The Norwegian Radium Hospital, Oslo
    Methods Mol Biol 252:569-80. 2004
    ..The established biopanning procedure should help in the rational selection of cancer-targeting peptides for specific delivery of DNA and RNA oligonucleotides into cancer cells...
  87. ncbi request reprint [Tyrosine kinase receptor-ras-ERK signal transduction pathway as therapeutic tarfet in cancer]
    Marianne Leirdal
    Avdeling for immunologi Gruppe for molekylaer medisin Institutt for Kreftforskning Det Norske Radiumhospital Montebello 0310 Oslo
    Tidsskr Nor Laegeforen 122:178-82. 2002
    ....
  88. ncbi request reprint Potential design rules and enzymatic synthesis of siRNAs
    Mouldy Sioud
    Department of Immunology, Molecular Medicine Group, The Norwegian Radium Hospital, Oslo
    Methods Mol Biol 252:457-69. 2004
    ..Here, potential design rules, cost-effective strategies for producing siRNAs by T7 RNA polymerase, and expression cassettes for in vivo testing are described...
  89. ncbi request reprint Treatment options and individualized medicine
    Mouldy Sioud
    Department of Immunology, Institute for Cancer Research, The Norwegian Radium Hospital, University of Oslo, Oslo, Norway
    Methods Mol Biol 361:327-40. 2007
    ..In this chapter, several relevant examples are presented...