Research Topics
| K SandvigSummaryAffiliation: The Norwegian Radium Hospital Country: Norway Publications
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Detail Information
Publications
Cellular trafficking of quantum dot-ligand bioconjugates and their induction of changes in normal routing of unconjugated ligandsChristina Tekle
Centre for Cancer Biomedicine, Faculty Division, Norwegian Radium Hospital, University of Oslo, 0316 Oslo, Norway
Nano Lett 8:1858-65. 2008..Thus, the results demonstrate that Qdots may have severe consequences on cell physiology...- BiP Negatively Affects Ricin TransportTone F Gregers
Department of Biosciences, and Centre for Immune Regulation, University of Oslo, Oslo 0316, Norway
Toxins (Basel) 5:969-82. 2013..Our findings suggest that in contrast to what was shown with the Shiga toxin, the presence of BiP does not facilitate, but rather inhibits the entry of ricin into the cytosol... 
Membrane traffic exploited by protein toxinsKirsten Sandvig
Department of Biochemistry, Institute for Cancer Research, The Norwegian Radium Hospital, Montebello, 0310 Oslo, Norway
Annu Rev Cell Dev Biol 18:1-24. 2002..With increasing information about the internalization and intracellular trafficking of these opportunistic toxins, new avenues have been opened for their application in areas of medicine such as drug delivery and therapy...- Pathways followed by ricin and Shiga toxin into cellsKirsten Sandvig
Institute for Cancer Research, The Norwegian Radium Hospital, Montebello, 0310 Oslo, Norway
Histochem Cell Biol 117:131-41. 2002..Trafficking of protein toxins along these pathways will be discussed in the present article... - Clathrin-independent endocytosis: mechanisms and functionKirsten Sandvig
Department of Biochemistry, Institute for Cancer Research, The Norwegian Radium Hospital, Oslo University Hospital, Oslo, Norway
Curr Opin Cell Biol 23:413-20. 2011.... - Protein toxins from plants and bacteria: probes for intracellular transport and tools in medicineKirsten Sandvig
Department of Biochemistry, Institute for Cancer Research, The Norwegian Radium Hospital, Oslo University Hospital, Oslo, Norway
FEBS Lett 584:2626-34. 2010..Increased knowledge about toxins is important to prevent and treat these diseases in an optimal way. Interestingly, toxins can be used for diagnosis and treatment of cancer... - Ricin transport into cells: studies of endocytosis and intracellular transportK Sandvig
Department of Biochemistry, Institute for Cancer Research, The Norwegian Radium Hospital, Oslo
Int J Med Microbiol 290:415-20. 2000..By using ricin we have characterized different types of endocytosis and the transport steps used by this toxin... - Apical macropinocytosis in polarized MDCK cells: regulation by N-ethylmaleimide-sensitive proteinsK Sandvig
Institute for Cancer Research, The Norwegian Radium Hospital, Department of Biochemistry, Montebello, Oslo
Eur J Cell Biol 79:447-57. 2000..The results suggest that the fusogenic property of both the apical and the basolateral plasma membrane of MDCK cells differs from that typically observed in cells unable to polarize... - Transport of protein toxins into cells: pathways used by ricin, cholera toxin and Shiga toxinKirsten Sandvig
Institute for Cancer Research, The Norwegian Radium Hospital, Montebello, 0310, Oslo, Norway
FEBS Lett 529:49-53. 2002..The toxins can also be used as vectors to carry other proteins into the cells. Studies with protein toxins reveal that there are more pathways along the plasma membrane to ER route than originally believed... - Endocytosis and retrograde transport of Shiga toxinKirsten Sandvig
Department of Biochemistry, Institute for Cancer Research, The Norwegian Radium Hospital, Oslo University Hospital, Montebello, N 0310 Oslo, Norway
Toxicon 56:1181-5. 2010..This ribotoxic effect is in some cells followed by apoptosis. In this article we focus on new discoveries concerning endocytosis and retrograde transport of Shiga toxin to the Golgi, the ER and the cytosol... 
Clathrin-independent endocytosis: from nonexisting to an extreme degree of complexityKirsten Sandvig
Centre for Cancer Biomedicine, University of Oslo and Department of Biochemistry, The Norwegian Radium Hospital, Montebello, Oslo, Norway
Histochem Cell Biol 129:267-76. 2008....- Endocytosis and intracellular transport of ricin: recent discoveriesK Sandvig
Institute for Cancer Research, The Norwegian Radium Hospital, Montebello, Oslo
FEBS Lett 452:67-70. 1999..The different transport steps have been analyzed by studying trafficking of ricin as well as modified ricin molecules... - Pathways followed by protein toxins into cellsKirsten Sandvig
Department of Biochemistry, Institute for Cancer Research, The Norwegian Radium Hospital, Montebello, Oslo, Norway
Int J Med Microbiol 293:483-90. 2004.... - Shiga toxinsK Sandvig
Department of Biochemistry, Institute for Cancer Research, The Norwegian Radium Hospital, Montebello, 0310 Oslo, Norway
Toxicon 39:1629-35. 2001..An increasing knowledge about the toxins and their interactions with cells is important both for treatment of disease, and for elucidation of pathways of intracellular transport... - Endosome to Golgi transport of ricin is independent of clathrin and of the Rab9- and Rab11-GTPasesT G Iversen
Institute for Cancer Research, The Norwegian Radium Hospital, Montebello 0310 Oslo, Norway
Mol Biol Cell 12:2099-107. 2001..In conclusion, ricin is transported from endosomes to the Golgi apparatus by a Rab9-, Rab11-, and clathrin-independent pathway... - Internalization of cholera toxin by different endocytic mechanismsM L Torgersen
Institute for Cancer Research, The Norwegian Radium Hospital, Montebello, 0310 Oslo, Norway
J Cell Sci 114:3737-47. 2001..In conclusion, a large fraction of CT can be endocytosed by clathrin-dependent as well as by caveolae- and clathrin-independent endocytosis in different cell types... - Photochemical internalization: a novel technology for delivery of macromolecules into cytosolK Berg
Department of Biophysics, Institute for Cancer Research, The Norwegian Radium Hospital, Montebello, Oslo
Cancer Res 59:1180-3. 1999..g., in gene therapy, vaccination, and cancer treatment... - Photochemical internalisation increases the cytotoxic effect of the immunotoxin MOC31-geloninP K Selbo
Department of Biophysics, Institute for Cancer Research, The Norwegian Radium Hospital, Oslo, Norway
Int J Cancer 87:853-9. 2000..Our results indicate that PCI is a promising tool for increasing the cytotoxicity of immunotoxins, which is important for further improvement of the PCI concept towards possible use in cancer therapy... - Proteoglycan synthesis is increased in cells with impaired clathrin-dependent endocytosisA Llorente
Department of Biochemistry, The Norwegian Radium Hospital, Montebello, Norway
J Cell Sci 114:335-43. 2001..Thus, the activation of protein kinase C observed upon inhibition of clathrin-dependent endocytosis may be responsible for the increased synthesis of proteoglycans... - Selective modulation of the endocytic uptake of ricin and fluid phase markers without alteration in transferrin endocytosisK Sandvig
Institute for Cancer Research, Norwegian Radium Hospital, Montebello, Oslo
J Biol Chem 265:6382-8. 1990..The data support the view that there are different endocytic mechanisms, and they indicate that at least in some cell types the non-clathrin-coated endocytosis can be modulated... - Reconstitution of clathrin-independent endocytosis at the apical domain of permeabilized MDCK II cells: requirement for a Rho-family GTPaseØ Garred
Institute for Cancer Research, The Norwegian Radium Hospital, Montebello, 0310 Oslo, Norway
Traffic 2:26-36. 2001.... - Release of gelonin from endosomes and lysosomes to cytosol by photochemical internalizationP K Selbo
Department of Biophysics, Institute for Cancer Research, The Norwegian Radium Hospital, Montebello, Oslo, Norway
Biochim Biophys Acta 1475:307-13. 2000..Thus, although gelonin can enter the cytosol from lysosomes, lysosomal degradation is a limiting factor for the outcome of PCI of gelonin... - Toxin-induced cell lysis: protection by 3-methyladenine and cycloheximideK Sandvig
Institute for Cancer Research, Norwegian Radium Hospital, Montebello, Oslo
Exp Cell Res 200:253-62. 1992..The data suggest that autophagy is important for the toxin-induced cell lysis... - Delivery into cells: lessons learned from plant and bacterial toxinsK Sandvig
Institute for Cancer Research, The Norwegian Radium Hospital, University of Oslo, Montebello
Gene Ther 12:865-72. 2005..In conclusion, the protein toxins are useful tools to study transport and cytosolic translocation, and they can be used as vectors for transport to the interior of the cell... - Penetration of protein toxins into cellsP O Falnes
Department of Biochemistry, Institute for Cancer Research, The Norwegian Radium Hospital, Montebello, Oslo, 0310, Norway
Curr Opin Cell Biol 12:407-13. 2000..In addition, the intracellular targets of many of these toxins have been identified recently... - Clathrin-coated pits with long, dynamin-wrapped necks upon expression of a clathrin antisense RNAT-G Iversen
Institute for Cancer Research at The Norwegian Radium Hospital, Montebello, N-0310 Oslo, Norway
Proc Natl Acad Sci U S A 100:5175-80. 2003.... - In vivo documentation of photochemical internalization, a novel approach to site specific cancer therapyP K Selbo
Department of Biophysics, Institute for Cancer Research, The Norwegian Radium Hospital, Montebello, N 0310 Oslo, Norway
Int J Cancer 92:761-6. 2001..These results demonstrate that the synergistic effect of combining photoactivation of photosensitizer located in endocytic vesicles and gelonin is indeed a result of PCI of gelonin... - Depletion of sphingolipids facilitates endosome to Golgi transport of ricinStine Grimmer
Institute for Cancer Research, Department of Biochemistry, The Norwegian Radium Hospital, University of Oslo, Montebello, 0310 Oslo, Norway
Traffic 7:1243-53. 2006..Our results indicate that the presence of sphingolipids actually limits and may function to control endosome to Golgi transport of ricin... - Phosphoinositide-regulated retrograde transport of ricin: crosstalk between hVps34 and sorting nexinsSigrid S Skånland
Department of Biochemistry, Institute for Cancer Research, University of Oslo, Faculty Department The Norwegian Radium Hospital, Montebello, N 0310 Oslo, Norway
Traffic 8:297-309. 2007..Interestingly, knocking down both SNX2 and SNX4 results in a better inhibition than knocking down only one of them, suggesting that they may act on separate pathways... - EDEM is involved in retrotranslocation of ricin from the endoplasmic reticulum to the cytosolMonika Slominska-Wojewodzka
Department of Biochemistry, Institute for Cancer Research, Faculty Division The Norwegian Radium Hospital, University of Oslo, Montebello, N 0310 Oslo, Norway
Mol Biol Cell 17:1664-75. 2006..In conclusion, our results indicate that EDEM is involved in retrotranslocation of ricin from the ER to the cytosol... - Transport of ricin from endosomes to the Golgi apparatus is regulated by Rab6A and Rab6A'Audrun Utskarpen
Department of Biochemistry, Institute for Cancer Research, Faculty Division The Norwegian Radium Hospital, University of Oslo, Montebello, 0310 Oslo, Norway
Traffic 7:663-72. 2006..In conclusion, both Rab6A and Rab6A' seem to be involved in the transport of ricin from endosomes to the Golgi apparatus... - SNX1 and SNX2 mediate retrograde transport of Shiga toxinAudrun Utskarpen
Institute for Cancer Research, Centre for Cancer Biomedicine, The Norwegian Radium Hospital, Montebello, Oslo, Norway
Biochem Biophys Res Commun 358:566-70. 2007..Inhibition of PI(3)P formation by wortmannin resulted in a similar reduction. Thus, although being partly redundant, both SNX1 and SNX2 are required for efficient Stx trafficking to the Golgi apparatus... - Polyunsaturated fatty acids regulate Shiga toxin transportBjørn Spilsberg
Department of Biochemistry, Centre for Cancer Biomedicine, Institute for Cancer Research, Faculty Division The Norwegian Radium Hospital, University of Oslo, Montebello, N 0310 Oslo, Norway
Biochem Biophys Res Commun 364:283-8. 2007..In conclusion, these results clearly demonstrate the importance of PUFA for Stx and cholera toxin trafficking... - The Mitogen-activated protein kinase p38 links Shiga Toxin-dependent signaling and traffickingSebastien Walchli
Department of Biochemistry and Centre for Cancer Biomedicine, Institute for Cancer Research, The Norwegian Radium Hospital, University of Oslo, Montebello, N 0310 Oslo, Norway
Mol Biol Cell 19:95-104. 2008..Intracellular transport of Stx is Ca(2+) dependent, and we provide evidence that Stx activates a signaling cascade involving cross talk between Ca(2+) and p38, to regulate its trafficking to the Golgi apparatus... - Protein kinase Cdelta is activated by Shiga toxin and regulates its transportMaria L Torgersen
Institute for Cancer Research, Faculty Division, The Norwegian Radium Hospital, University of Oslo, Montebello, 0310 Oslo, Norway
J Biol Chem 282:16317-28. 2007..These results are in agreement with our previous data, which show that Stx is able to stimulate its own transport... - Shiga toxin regulates its entry in a Syk-dependent mannerSilje Ugland Lauvrak
Department of Biochemistry, Institute for Cancer Research, The Norwegian Radium Hospital, 0310 Oslo, Norway
Mol Biol Cell 17:1096-109. 2006..Also, Golgi transport of Stx was inhibited under all these conditions. In conclusion, our data suggest that Stx regulates its entry into target cells... - Induction of direct endosome to endoplasmic reticulum transport in Chinese hamster ovary (CHO) cells (LdlF) with a temperature-sensitive defect in epsilon-coatomer protein (epsilon-COP)Alicia Llorente
Institute for Cancer Research, The Norwegian Radium Hospital, Montebello, 0310 Oslo, Norway
J Biol Chem 278:35850-5. 2003..In conclusion, our results suggest that in ldlF cells depleted of epsilon-COP ricin might be transported to the ER by an induced brefeldin A-resistant pathway that circumvents the Golgi apparatus... - Cellular internalization of cytolethal distending toxin: a new end to a known pathwayLina Guerra
Microbiology and Tumorbiology Center, Karolinska Institutet, Stockholm, Sweden
Cell Microbiol 7:921-34. 2005.... - Role of lipids in the retrograde pathway of ricin intoxicationBjørn Spilsberg
Institute for Cancer Research, The Norwegian Radium Hospital, Montebello, N 0310 Oslo, Norway
Traffic 4:544-52. 2003..Binding of ricin to glycoproteins is sufficient for all transport steps required for ricin intoxication... - Efficient endosome-to-Golgi transport of Shiga toxin is dependent on dynamin and clathrinSilje U Lauvrak
Institute for Cancer Research, The Norwegian Radium Hospital, Montebello, 0310 Oslo, Norway
J Cell Sci 117:2321-31. 2004.... - Caveolae: anchored, multifunctional platforms in the lipid oceanBo van Deurs
Structural Cell Biology Unit, Department of Medical Anatomy, The Panum Institute, University of Copenhagen, Denmark
Trends Cell Biol 13:92-100. 2003..Rather than having a specific function, caveolae might be considered to be multifunctional organelles with a physiological role that varies depending on cell type and cellular needs... - Differential role of actin, clathrin, and dynamin in Fc gamma receptor-mediated endocytosis and phagocytosisShirley M L Tse
Department of Pediatrics, Division of Rheumatology, Hospital for Sick Children, Institute of Medical Science, and University Health Network, University of Toronto, Toronto, Ontario M5G 1X8, Canada
J Biol Chem 278:3331-8. 2003..These results imply that, contrary to prevailing notions, normal dynamin and clathrin function is not required for phagocytosis and reveal the existence of a component of phagocytosis that is independent of actin and Rab5... - Endosome-to-Golgi transport is regulated by protein kinase A type II alphaKim Are Birkeli
Institute for Cancer Research, The Norwegian Radium Hospital, Montebello, 0310 Oslo, Norway
J Biol Chem 278:1991-7. 2003..In conclusion, our results demonstrate that transport of ricin from endosomes to the Golgi apparatus and further to the ER is regulated by PKA type II alpha isozyme... - Selective regulation of the Rab9-independent transport of ricin to the Golgi apparatus by calciumSilje U Lauvrak
Institute for Cancer Research, The Norwegian Radium Hospital, Montebello, 0310 Oslo, Norway
J Cell Sci 115:3449-56. 2002..Together, these findings indicate that the different pathways to the Golgi apparatus utilized by ricin and the mannose 6-phosphate receptor are regulated by different mechanisms... - Membrane ruffling and macropinocytosis in A431 cells require cholesterolStine Grimmer
Institute for Cancer Research, The Norwegian Radium Hospital, Montebello, 0310 Oslo, Norway
J Cell Sci 115:2953-62. 2002..Thus, our results demonstrate that cholesterol is required for the membrane localization of activated Rac1, actin reorganization, membrane ruffling and macropinocytosis... - Caveolae: stable membrane domains with a potential for internalizationAnette M Hommelgaard
Structural Cell Biology Unit, Department of Medical Anatomy, The Panum Institute, University of Copenhagen, DK-2200 Copenhagen N, Denmark
Traffic 6:720-4. 2005..Finally, we propose that future studies dealing with internalization of caveolae should actually document such internalization and include kinetic data... - Productive entry of type C foot-and-mouth disease virus into susceptible cultured cells requires clathrin and is dependent on the presence of plasma membrane cholesterolMiguel A Martín Acebes
Centro de Biologia Molecular Severo Ochoa CSIC UAM, Cantoblanco 28049, Madrid, Spain
Virology 369:105-18. 2007.... - Cholesterol regulates prostasome release from secretory lysosomes in PC-3 human prostate cancer cellsAlicia Llorente
Centre for Cancer Biomedicine, Faculty Division The Norwegian Radium Hospital, University of Oslo, Rikshospitalet Radiumhospitalet Medical Centre, N 0310 Oslo, Norway
Eur J Cell Biol 86:405-15. 2007.... - Structural requirements for furin-induced cleavage and activation of Shiga toxinAlma Kurmanova
W A Engelhardt Institute for Molecular Biology, The Russian Academy of Sciences, Moscow, Russia
Biochem Biophys Res Commun 357:144-9. 2007..In contrast, LoVo/neo cells lacking functional furin were unable to activate both wild type Shiga toxin and Shiga-2D toxin. In conclusion, an extended loop structure is required for furin-induced cleavage of Shiga toxin... - The Cbl-interacting protein TULA inhibits dynamin-dependent endocytosisVibeke Bertelsen
Institute of Pathology, Faculty Division Rikshospitalet, University of Oslo, Rikshospitalet Radiumhospitalet Medical Centre, 0027 Oslo, Norway
Exp Cell Res 313:1696-709. 2007..We conclude that TULA inhibits both clathrin-dependent and clathrin-independent endocytic pathways by functionally sequestering dynamin via the SH3 domain of TULA binding proline-rich sequences in dynamin... - Golgi vesiculation induced by cholesterol occurs by a dynamin- and cPLA2-dependent mechanismStine Grimmer
Institute for Cancer Research, Department of Biochemistry, The Norwegian Radium Hospital, Montebello, 0310 Oslo, Norway
Traffic 6:144-56. 2005..Thus, our results demonstrate that the cholesterol-induced vesiculation of the Golgi apparatus is mediated by a cPLA2- and dynamin-dependent mechanism... - Diphtheria toxin translocation across cellular membranes is regulated by sphingolipidsBjørn Spilsberg
Institute for Cancer Research, The Norwegian Radium Hospital, Montebello, N 0310 Oslo, Norway
Biochem Biophys Res Commun 329:465-73. 2005..In conclusion, diphtheria toxin translocation is facilitated by depletion of membrane sphingolipids... - Cholesterol loading induces a block in the exit of VSVG from the TGNMing Ying
Institute for Cancer Research, The Norwegian Radium Hospital, Montebello, 0310 Oslo, Norway
Traffic 4:772-84. 2003..Furthermore, the data illustrate the importance of actin filaments for Golgi structure... - The A-subunit of surface-bound Shiga toxin stimulates clathrin-dependent uptake of the toxinMaria L Torgersen
Institute for Cancer Research, The Norwegian Radium Hospital, Montebello, Oslo, Norway
FEBS J 272:4103-13. 2005..In conclusion, the data indicate that the Shiga toxin A-subunit of cell surface-bound toxin stimulates clathrin-dependent uptake of the toxin. Possible explanations for this phenomenon are discussed... - Sequestration of epidermal growth factor receptors in non-caveolar lipid rafts inhibits ligand bindingKirstine Roepstorff
Structural Cell Biology Unit, Department of Medical Anatomy, The Panum Institute, University of Copenhagen, DK 2200 Copenhagen N, Denmark
J Biol Chem 277:18954-60. 2002..We propose that non-caveolar lipid rafts function as negative regulators of EGF receptor signaling by sequestering a fraction of the EGF receptors in a state inaccessible for ligand binding...