Bodil Kavli

Summary

Affiliation: Norwegian University of Science and Technology
Country: Norway

Publications

  1. pmc Uracil-DNA glycosylases SMUG1 and UNG2 coordinate the initial steps of base excision repair by distinct mechanisms
    Henrik Sahlin Pettersen
    Department of Cancer Research and Molecular Medicine, NTNU, N 7006 Trondheim, Norway
    Nucleic Acids Res 35:3879-92. 2007
  2. ncbi request reprint Uracil in DNA--general mutagen, but normal intermediate in acquired immunity
    Bodil Kavli
    Department of Cancer Research and Molecular Medicine, Faculty of Medicine, Norwegian University of Science and Technology, N 7006 Trondheim, Norway
    DNA Repair (Amst) 6:505-16. 2007
  3. doi request reprint The UNG2 Arg88Cys variant abrogates RPA-mediated recruitment of UNG2 to single-stranded DNA
    Kathrin Torseth
    Department of Cancer Research and Molecular Medicine, Faculty of Medicine, the FUGE Proteomics Node, Norwegian University of Science and Technology, N 7006 Trondheim, Norway
    DNA Repair (Amst) 11:559-69. 2012
  4. pmc UNG-initiated base excision repair is the major repair route for 5-fluorouracil in DNA, but 5-fluorouracil cytotoxicity depends mainly on RNA incorporation
    Henrik Sahlin Pettersen
    Department of Cancer Research and Molecular Medicine, Norwegian University of Science and Technology, N 7489 Trondheim, Norway
    Nucleic Acids Res 39:8430-44. 2011
  5. pmc Human AlkB homolog 1 is a mitochondrial protein that demethylates 3-methylcytosine in DNA and RNA
    Marianne Pedersen Westbye
    Department of Cancer Research and Molecular Medicine, Norwegian University of Science and Technology, N 7489 Trondheim, Norway
    J Biol Chem 283:25046-56. 2008
  6. ncbi request reprint Alkylation damage in DNA and RNA--repair mechanisms and medical significance
    Finn Drabløs
    Department of Cancer Research and Molecular Medicine, Norwegian University of Science and Technology, N 7489 Trondheim, Norway
    DNA Repair (Amst) 3:1389-407. 2004
  7. doi request reprint A combined nuclear and nucleolar localization motif in activation-induced cytidine deaminase (AID) controls immunoglobulin class switching
    Yi Hu
    Department of Cancer Research and Molecular Medicine, Norwegian University of Science and Technology, N 7491 Trondheim, Norway
    J Mol Biol 425:424-43. 2013
  8. doi request reprint Strikingly different properties of uracil-DNA glycosylases UNG2 and SMUG1 may explain divergent roles in processing of genomic uracil
    Berit Doseth
    Department of Cancer Research and Molecular Medicine, Faculty of Medicine, Norwegian University of Science and Technology, Trondheim, Norway
    DNA Repair (Amst) 11:587-93. 2012
  9. pmc Repair of U/G and U/A in DNA by UNG2-associated repair complexes takes place predominantly by short-patch repair both in proliferating and growth-arrested cells
    Mansour Akbari
    Department of Cancer Research and Molecular Medicine, Norwegian University of Science and Technology, N 7489 Trondheim, Norway
    Nucleic Acids Res 32:5486-98. 2004
  10. pmc Uracil in DNA and its processing by different DNA glycosylases
    Torkild Visnes
    Department of Cancer Research and Molecular Medicine, Norwegian University of Science and Technology, 7489 Trondheim, Norway
    Philos Trans R Soc Lond B Biol Sci 364:563-8. 2009

Collaborators

Detail Information

Publications23

  1. pmc Uracil-DNA glycosylases SMUG1 and UNG2 coordinate the initial steps of base excision repair by distinct mechanisms
    Henrik Sahlin Pettersen
    Department of Cancer Research and Molecular Medicine, NTNU, N 7006 Trondheim, Norway
    Nucleic Acids Res 35:3879-92. 2007
    ..UNG2 is apparently adapted to rapid and highly coordinated repair of uracil (U:G and U:A) in replicating DNA, while the less efficient SMUG1 may be more important in repair of deaminated cytosine (U:G) in non-replicating chromatin...
  2. ncbi request reprint Uracil in DNA--general mutagen, but normal intermediate in acquired immunity
    Bodil Kavli
    Department of Cancer Research and Molecular Medicine, Faculty of Medicine, Norwegian University of Science and Technology, N 7006 Trondheim, Norway
    DNA Repair (Amst) 6:505-16. 2007
    ..Ung(-/-) mice have a similar phenotype, but in addition display dysregulated cytokine production and develop B cell lymphomas late in life...
  3. doi request reprint The UNG2 Arg88Cys variant abrogates RPA-mediated recruitment of UNG2 to single-stranded DNA
    Kathrin Torseth
    Department of Cancer Research and Molecular Medicine, Faculty of Medicine, the FUGE Proteomics Node, Norwegian University of Science and Technology, N 7006 Trondheim, Norway
    DNA Repair (Amst) 11:559-69. 2012
    ....
  4. pmc UNG-initiated base excision repair is the major repair route for 5-fluorouracil in DNA, but 5-fluorouracil cytotoxicity depends mainly on RNA incorporation
    Henrik Sahlin Pettersen
    Department of Cancer Research and Molecular Medicine, Norwegian University of Science and Technology, N 7489 Trondheim, Norway
    Nucleic Acids Res 39:8430-44. 2011
    ..In conclusion, UNG-initiated BER is the major route for FU-DNA repair, but cytotoxicity of FU is predominantly RNA-mediated, while DNA-mediated effects are limited to FdUrd...
  5. pmc Human AlkB homolog 1 is a mitochondrial protein that demethylates 3-methylcytosine in DNA and RNA
    Marianne Pedersen Westbye
    Department of Cancer Research and Molecular Medicine, Norwegian University of Science and Technology, N 7489 Trondheim, Norway
    J Biol Chem 283:25046-56. 2008
    ..In conclusion, hABH1 is a functional mitochondrial AlkB homolog that repairs 3-methylcytosine in single-stranded DNA and RNA...
  6. ncbi request reprint Alkylation damage in DNA and RNA--repair mechanisms and medical significance
    Finn Drabløs
    Department of Cancer Research and Molecular Medicine, Norwegian University of Science and Technology, N 7489 Trondheim, Norway
    DNA Repair (Amst) 3:1389-407. 2004
    ..An evaluation of the biological effects of environmental mutagens, as well as understanding the mechanism of action and resistance to alkylating drugs require a detailed understanding of DNA repair processes...
  7. doi request reprint A combined nuclear and nucleolar localization motif in activation-induced cytidine deaminase (AID) controls immunoglobulin class switching
    Yi Hu
    Department of Cancer Research and Molecular Medicine, Norwegian University of Science and Technology, N 7491 Trondheim, Norway
    J Mol Biol 425:424-43. 2013
    ..In addition, our results resolve the problem related to dissociation of deamination activity and CSR activity of AID mutants...
  8. doi request reprint Strikingly different properties of uracil-DNA glycosylases UNG2 and SMUG1 may explain divergent roles in processing of genomic uracil
    Berit Doseth
    Department of Cancer Research and Molecular Medicine, Faculty of Medicine, Norwegian University of Science and Technology, Trondheim, Norway
    DNA Repair (Amst) 11:587-93. 2012
    ..We suggest a model for mutagenic processing in which replication protein A (RPA) recruits UNG2 to sites of deamination and keeps DNA in a single stranded conformation, thus avoiding error-free BER of the deaminated cytosine...
  9. pmc Repair of U/G and U/A in DNA by UNG2-associated repair complexes takes place predominantly by short-patch repair both in proliferating and growth-arrested cells
    Mansour Akbari
    Department of Cancer Research and Molecular Medicine, Norwegian University of Science and Technology, N 7489 Trondheim, Norway
    Nucleic Acids Res 32:5486-98. 2004
    ..In conclusion, UNG2 is present in preassembled complexes proficient in BER. Furthermore, UNG2 is the major enzyme initiating BER of deaminated cytosine (U/G), and possibly the sole enzyme initiating BER of misincorporated uracil (U/A)...
  10. pmc Uracil in DNA and its processing by different DNA glycosylases
    Torkild Visnes
    Department of Cancer Research and Molecular Medicine, Norwegian University of Science and Technology, 7489 Trondheim, Norway
    Philos Trans R Soc Lond B Biol Sci 364:563-8. 2009
    ..There are also some indications that there may be species differences in the function of the uracil-DNA glycosylases...
  11. ncbi request reprint Genomic uracil and human disease
    Lars Hagen
    Department of Cancer Research and Molecular Medicine, Faculty of Medicine, Norwegian University of Science and Technology, Trondheim
    Exp Cell Res 312:2666-72. 2006
    ..Ung(-/-) mice have a similar phenotype and develop B-cell lymphomas late in life. However, there is no evidence indicating that UNG deficiency causes lymphomas in humans...
  12. pmc Cell cycle-specific UNG2 phosphorylations regulate protein turnover, activity and association with RPA
    Lars Hagen
    Department of Cancer Research and Molecular Medicine, Norwegian University of Science and Technology, Trondheim, Norway
    EMBO J 27:51-61. 2008
    ..Our findings may aid further studies of how UNG2 initiates mutagenic rather than repair processing of activation-induced deaminase-generated uracil at Ig loci in B cells...
  13. ncbi request reprint Activation-induced cytidine deaminase (AID) is localized to subnuclear domains enriched in splicing factors
    Yi Hu
    Central Norway Health Authorities and Department of Cancer Research and Molecular Medicine, Faculty of Medicine, NTNU, N 7491, Norwegian University of Science and Technology, Trondheim, Norway Electronic address
    Exp Cell Res 322:178-92. 2014
    ..Based on our findings and the literature, we suggest a transcription-coupled splicing-associated model for AID targeting and activation. ..
  14. ncbi request reprint hUNG2 is the major repair enzyme for removal of uracil from U:A matches, U:G mismatches, and U in single-stranded DNA, with hSMUG1 as a broad specificity backup
    Bodil Kavli
    Institute of Cancer Research and Molecular Biology, Norwegian University of Science and Technology, N 7489 Trondheim, Norway
    J Biol Chem 277:39926-36. 2002
    ..We also provide evidence that hUNG2 is the major enzyme for removal of deaminated cytosine outside of replication foci, with hSMUG1 acting as a broad specificity backup...
  15. pmc B cells from hyper-IgM patients carrying UNG mutations lack ability to remove uracil from ssDNA and have elevated genomic uracil
    Bodil Kavli
    Department of Cancer Research and Molecular Medicine, Faculty of Medicine, Norwegian University of Science and Technology, N 7489 Trondheim, Norway
    J Exp Med 201:2011-21. 2005
    ..Based on our findings and recent information in the literature, we present an integrated model for the initiating steps in CSR...
  16. pmc Uracil-DNA glycosylase in base excision repair and adaptive immunity: species differences between man and mouse
    Berit Doseth
    Department of Cancer Research and Molecular Medicine, Faculty of Medicine, Norwegian University of Science and Technology, N 7491 Trondheim, Norway
    J Biol Chem 286:16669-80. 2011
    ..Our results reveal significant species differences in genomic uracil processing. These findings should be taken into account when mouse models are used in studies of uracil DNA repair and adaptive immunity...
  17. ncbi request reprint Expression and recruitment of uracil-DNA glycosylase are regulated by E2A during antibody diversification
    Anders Wallenius
    Department of Cancer Research and Molecular Medicine, Faculty of Medicine, Norwegian University of Science and Technology, N 7489, Trondheim, Norway Department of Molecular Biology, Umea University, 90187, Umea, Sweden
    Mol Immunol 60:23-31. 2014
    ..The results suggest that E2A is a key factor in regulating the balance between AID and UNG2, both at expression and Ig targeting levels, to stimulate Ig diversification and suppress normal DNA repair processes...
  18. ncbi request reprint The interacting pathways for prevention and repair of oxidative DNA damage
    Geir Slupphaug
    Institute of Cancer Research and Molecular Medicine, Norwegian University of Science and Technology, N 7489 Trondheim, Norway
    Mutat Res 531:231-51. 2003
    ..Thus, there is both inter- and intra-pathway complementation in repair of oxidative base damage, explaining the limited effects of absence of single DNA glycosylases in animal model systems...
  19. ncbi request reprint Error-free versus mutagenic processing of genomic uracil-Relevance to cancer
    Hans E Krokan
    Department of Cancer Research and Molecular Medicine, Norwegian University of Science and Technology, NO 7489 Trondheim, Norway Electronic address
    DNA Repair (Amst) 19:38-47. 2014
    ..In conclusion, genomic uracil is an essential intermediate in adaptive immunity and innate antiviral responses, but may also be a fundamental cause of a wide range of malignancies. ..
  20. ncbi request reprint Novel aspects of macromolecular repair and relationship to human disease
    Hans E Krokan
    Department of Cancer Research and Molecular Medicine, Norwegian University of Science and Technology, 7489 Trondheim, Norway
    J Mol Med (Berl) 82:280-97. 2004
    ..The new knowledge opens for interventions that are based on a deeper understanding of the mechanisms of defence...
  21. ncbi request reprint Xenopus CENP-A assembly into chromatin requires base excision repair proteins
    Samantha G Zeitlin
    Department of Biological Sciences, University of California San Diego, Mail Code 0322, La Jolla, CA 92093, USA
    DNA Repair (Amst) 4:760-72. 2005
    ..Conversely, inducing DNA damage increases the level of CENP-A detected on sperm chromatin. Our data suggest that base excision repair may be involved in assembly of this histone H3 variant...
  22. ncbi request reprint NEIL1 is the major DNA glycosylase that processes 5-hydroxyuracil in the proximity of a DNA single-strand break
    Jason L Parsons
    MRC Radiation and Genome Stability Unit, Harwell, Oxfordshire OX11 0RD, UK
    Biochemistry 46:4158-63. 2007
    ..Subsequently, we found that although both SMUG1 and NEIL1 are able to excise 5-OHU lesions located in the proximity of the 3'-end of a DNA SSB, NEIL1 is more efficient in the repair of these DNA lesions...
  23. pmc Characterisation of the substrate specificity of homogeneous vaccinia virus uracil-DNA glycosylase
    Natale Scaramozzino
    Laboratoire de Virologie, Centre de Recherches du Service de Santé des Armées CRSSA Emile Pardé, Grenoble, France
    Nucleic Acids Res 31:4950-7. 2003
    ..Interestingly, both viral and human enzymes preferentially excise uracil when it is opposite to cytosine. The present study provides the basis for the design of specific inhibitors for vUNG...