Rolf I Skotheim

Summary

Affiliation: Institute for Cancer Research
Country: Norway

Publications

  1. ncbi request reprint Transcriptome instability as a molecular pan-cancer characteristic of carcinomas
    Anita Sveen
    Department of Cancer Prevention, Institute for Cancer Research, The Norwegian Radium Hospital, Oslo University Hospital, P, O, Box 4953 Nydalen, Oslo NO 0424, Norway
    BMC Genomics 15:672. 2014
  2. pmc Systematic bioinformatic analysis of expression levels of 17,330 human genes across 9,783 samples from 175 types of healthy and pathological tissues
    Sami Kilpinen
    Medical Biotechnology, VTT Technical Research Centre and University of Turku, Itäinen Pitkäkatu 4C, Turku, Finland
    Genome Biol 9:R139. 2008
  3. pmc Transcriptome instability in colorectal cancer identified by exon microarray analyses: Associations with splicing factor expression levels and patient survival
    Anita Sveen
    Department of Cancer Prevention, Institute for Cancer Research, The Norwegian Radium Hospital, Oslo University Hospital, PO Box 4953 Nydalen, NO 0424 Oslo, Norway
    Genome Med 3:32. 2011
  4. pmc A universal assay for detection of oncogenic fusion transcripts by oligo microarray analysis
    Rolf I Skotheim
    Department of Cancer Prevention, Institute for Cancer Research, Norwegian Radium Hospital, Rikshospitalet University Hospital, Oslo, Norway
    Mol Cancer 8:5. 2009
  5. ncbi request reprint Differentiation of human embryonal carcinomas in vitro and in vivo reveals expression profiles relevant to normal development
    Rolf I Skotheim
    Department of Genetics, Institute for Cancer Research, The Norwegian Radium Hospital and University of Oslo, Norway
    Cancer Res 65:5588-98. 2005
  6. ncbi request reprint Novel genomic aberrations in testicular germ cell tumors by array-CGH, and associated gene expression changes
    Rolf I Skotheim
    Department of Cancer Prevention, Institute for Cancer Research, Rikshospitalet Radiumhospitalet Medical Center, Oslo, Norway
    Cell Oncol 28:315-26. 2006
  7. pmc Gene methylation profiles of normal mucosa, and benign and malignant colorectal tumors identify early onset markers
    Terje Ahlquist
    Department of Cancer Prevention, Institute for Cancer Research, Norwegian Radium Hospital, Rikshospitalet University Hospital, Oslo, Norway
    Mol Cancer 7:94. 2008
  8. pmc The loss of NKX3.1 expression in testicular--and prostate--cancers is not caused by promoter hypermethylation
    Guro E Lind
    Department of Genetics, Institute for Cancer Research, The Norwegian Radium Hospital, 0310 Oslo, Norway
    Mol Cancer 4:8. 2005
  9. doi request reprint Genomic changes in chromosomes 10, 16, and X in malignant peripheral nerve sheath tumors identify a high-risk patient group
    Helge R Brekke
    Department of Cancer Prevention, Institute for Cancer Research, The Norwegian Radium Hospital, Rikshospitalet, Oslo University Hospital, Oslo, Norway
    J Clin Oncol 28:1573-82. 2010
  10. doi request reprint Array-CGH analysis of microdissected chromosome 19 markers in ovarian carcinoma identifies candidate target genes
    Francesca Micci
    Section for Cancer Cytogenetics, Institute for Medical Informatics, The Norwegian Radium Hospital, Oslo University Hospital, Montebello, Oslo, Norway
    Genes Chromosomes Cancer 49:1046-53. 2010

Collaborators

Detail Information

Publications45

  1. ncbi request reprint Transcriptome instability as a molecular pan-cancer characteristic of carcinomas
    Anita Sveen
    Department of Cancer Prevention, Institute for Cancer Research, The Norwegian Radium Hospital, Oslo University Hospital, P, O, Box 4953 Nydalen, Oslo NO 0424, Norway
    BMC Genomics 15:672. 2014
    ..We have previously proposed transcriptome instability as a genome-wide, pre-mRNA splicing-related characteristic of colorectal cancer. Here, we explore the hypothesis of transcriptome instability being a general characteristic of cancer...
  2. pmc Systematic bioinformatic analysis of expression levels of 17,330 human genes across 9,783 samples from 175 types of healthy and pathological tissues
    Sami Kilpinen
    Medical Biotechnology, VTT Technical Research Centre and University of Turku, Itäinen Pitkäkatu 4C, Turku, Finland
    Genome Biol 9:R139. 2008
    ..This database of gene expression patterns in normal human tissues and pathological conditions covers 113 million datapoints and is available from the GeneSapiens website...
  3. pmc Transcriptome instability in colorectal cancer identified by exon microarray analyses: Associations with splicing factor expression levels and patient survival
    Anita Sveen
    Department of Cancer Prevention, Institute for Cancer Research, The Norwegian Radium Hospital, Oslo University Hospital, PO Box 4953 Nydalen, NO 0424 Oslo, Norway
    Genome Med 3:32. 2011
    ..In the present study we analyze genome-wide disruption of pre-mRNA splicing, and propose transcriptome instability as a characteristic that is analogous to genomic instability on the transcriptome level...
  4. pmc A universal assay for detection of oncogenic fusion transcripts by oligo microarray analysis
    Rolf I Skotheim
    Department of Cancer Prevention, Institute for Cancer Research, Norwegian Radium Hospital, Rikshospitalet University Hospital, Oslo, Norway
    Mol Cancer 8:5. 2009
    ..However, the available methodologies to detect such fusions all have their distinct short-comings...
  5. ncbi request reprint Differentiation of human embryonal carcinomas in vitro and in vivo reveals expression profiles relevant to normal development
    Rolf I Skotheim
    Department of Genetics, Institute for Cancer Research, The Norwegian Radium Hospital and University of Oslo, Norway
    Cancer Res 65:5588-98. 2005
    ....
  6. ncbi request reprint Novel genomic aberrations in testicular germ cell tumors by array-CGH, and associated gene expression changes
    Rolf I Skotheim
    Department of Cancer Prevention, Institute for Cancer Research, Rikshospitalet Radiumhospitalet Medical Center, Oslo, Norway
    Cell Oncol 28:315-26. 2006
    ..Testicular germ cell tumors of adolescent and young adult men (TGCTs) generally have near triploid and complex karyotypes. The actual genes driving the tumorigenesis remain essentially to be identified...
  7. pmc Gene methylation profiles of normal mucosa, and benign and malignant colorectal tumors identify early onset markers
    Terje Ahlquist
    Department of Cancer Prevention, Institute for Cancer Research, Norwegian Radium Hospital, Rikshospitalet University Hospital, Oslo, Norway
    Mol Cancer 7:94. 2008
    ..Possible CIMP tumors were identified by comparing the methylation profile with microsatellite instability (MSI), BRAF-, KRAS-, and TP53 mutation status...
  8. pmc The loss of NKX3.1 expression in testicular--and prostate--cancers is not caused by promoter hypermethylation
    Guro E Lind
    Department of Genetics, Institute for Cancer Research, The Norwegian Radium Hospital, 0310 Oslo, Norway
    Mol Cancer 4:8. 2005
    ....
  9. doi request reprint Genomic changes in chromosomes 10, 16, and X in malignant peripheral nerve sheath tumors identify a high-risk patient group
    Helge R Brekke
    Department of Cancer Prevention, Institute for Cancer Research, The Norwegian Radium Hospital, Rikshospitalet, Oslo University Hospital, Oslo, Norway
    J Clin Oncol 28:1573-82. 2010
    ..The purpose of this study was to identify genetic aberrations contributing to clinical aggressiveness of malignant peripheral nerve sheath tumors (MPNSTs)...
  10. doi request reprint Array-CGH analysis of microdissected chromosome 19 markers in ovarian carcinoma identifies candidate target genes
    Francesca Micci
    Section for Cancer Cytogenetics, Institute for Medical Informatics, The Norwegian Radium Hospital, Oslo University Hospital, Montebello, Oslo, Norway
    Genes Chromosomes Cancer 49:1046-53. 2010
    ....
  11. doi request reprint Transforming pluripotency: an exon-level study of malignancy-specific transcripts in human embryonal carcinoma and embryonic stem cells
    Sharmini Alagaratnam
    Department of Cancer Prevention, Institute for Cancer Research, Oslo University Hospital, Oslo, Norway
    Stem Cells Dev 22:1136-46. 2013
    ..These findings are of particular interest for both GCT and ES cell biology, and, in general, to the concept of CSCs...
  12. doi request reprint ColoGuidePro: a prognostic 7-gene expression signature for stage III colorectal cancer patients
    Anita Sveen
    Department of Cancer Prevention, Institute for Cancer Research, The Norwegian Radium Hospital, Oslo, Norway
    Clin Cancer Res 18:6001-10. 2012
    ..This study was conducted to develop a clinically feasible and robust prognostic classifier for these patients independent of adjuvant treatment...
  13. doi request reprint The exon-level biomarker SLC39A14 has organ-confined cancer-specificity in colorectal cancer
    Anita Sveen
    Department of Cancer Prevention, Institute for Cancer Research, The Norwegian Radium Hospital, Oslo University Hospital, Oslo, Norway
    Int J Cancer 131:1479-85. 2012
    ..In conclusion, the SLC39A14-exon4B transcript variant is a CRC biomarker with high sensitivity and organ-confined specificity. Further use of the transcript and its encoded protein isoform should be explored in an organ-confined context...
  14. pmc Identification of an epigenetic biomarker panel with high sensitivity and specificity for colorectal cancer and adenomas
    Guro E Lind
    Department of Cancer Prevention, Institute for Cancer Research, Oslo University Hospital Radiumhospitalet, Oslo, Norway
    Mol Cancer 10:85. 2011
    ..This study investigates a panel of epigenetic markers for the detection of colorectal cancer and adenomas...
  15. ncbi request reprint ColoGuideEx: a robust gene classifier specific for stage II colorectal cancer prognosis
    Trude H Agesen
    Department of Cancer Prevention, Institute for Cancer Research, The Norwegian Radium Hospital, Oslo University Hospital, Nydalen, Oslo, Norway
    Gut 61:1560-7. 2012
    ..The present study aimed to develop a gene expression classifier for improved risk stratification of patients with stage II CRC...
  16. pmc Distinct high resolution genome profiles of early onset and late onset colorectal cancer integrated with gene expression data identify candidate susceptibility loci
    Marianne Berg
    Department of Cancer Prevention, Institute for Cancer Research, Oslo University Hospital, Norwegian Radium Hospital, Oslo, Norway
    Mol Cancer 9:100. 2010
    ..Integration analysis of CNV and genome wide mRNA expression data, available for the same tumors, was performed to identify a restricted candidate gene list...
  17. pmc Phospholipase C isozymes are deregulated in colorectal cancer--insights gained from gene set enrichment analysis of the transcriptome
    Stine A Danielsen
    Department of Cancer Prevention, Institute for Cancer Research, Oslo University Hospital, Oslo, Norway
    PLoS ONE 6:e24419. 2011
    ..In conclusion, we have identified significantly deregulated pathways in CRC, and validated repression of PLCD1 and PLCE1 expression. This illustrates that the GSEA approach may guide discovery of novel biomarkers in cancer...
  18. pmc Whole-transcriptome sequencing identifies novel IRF2BP2-CDX1 fusion gene brought about by translocation t(1;5)(q42;q32) in mesenchymal chondrosarcoma
    Kaja B Nyquist
    Section for Cancer Cytogenetics, Institute for Medical Informatics, The Norwegian Radium Hospital, Oslo University Hospital, Oslo, Norway
    PLoS ONE 7:e49705. 2012
    ..This demonstrates that genetic heterogeneity exists in mesenchymal chondrosarcoma...
  19. pmc Assessment of fusion gene status in sarcomas using a custom made fusion gene microarray
    Marthe Løvf
    Department of Cancer Prevention, Institute for Cancer Research, Norwegian Radium Hospital, Oslo University Hospital, Oslo, Norway
    PLoS ONE 8:e70649. 2013
    ..The analytical sensitivity may be further optimised, but we conclude that a sarcoma-fusion gene microarray is suitable as a time-saving screening tool to identify the majority of the correct fusion genes. ..
  20. ncbi request reprint Topoisomerase-II alpha is upregulated in malignant peripheral nerve sheath tumors and associated with clinical outcome
    Rolf I Skotheim
    Department of Genetics, Institute for Cancer Research, The Norwegian Radium Hospital and University of Oslo, Norway
    J Clin Oncol 21:4586-91. 2003
    ..To identify target genes of clinical significance for patients with malignant peripheral-nerve sheath tumor (MPNST), an aggressive cancer for which no consensus therapy exists...
  21. pmc Candidate genes for testicular cancer evaluated by in situ protein expression analyses on tissue microarrays
    Rolf I Skotheim
    Department of Genetics, Institute for Cancer Research, The Norwegian Radium Hospital, Oslo, Norway
    Neoplasia 5:397-404. 2003
    ..In summary, we have developed a high-throughput tool for the evaluation of TGCT markers, and utilized this to validate five candidate genes whose protein expressions were indeed deregulated in TGCT...
  22. pmc MiR-9, -31, and -182 deregulation promote proliferation and tumor cell survival in colon cancer
    Lina Cekaite
    Department of Cancer Prevention, Institute for Cancer Research, Norwegian Radium Hospital, Oslo University Hospital, Oslo, Norway
    Neoplasia 14:868-79. 2012
    ..This study suggests that deregulated expression of miR-9, miR-31, and miR-182 during carcinogenesis plays a significant role in the development of colon cancer by promoting proliferation and tumor cell survival...
  23. pmc Hypermethylated MAL gene - a silent marker of early colon tumorigenesis
    Guro E Lind
    Department of Cancer Prevention, Institute for Cancer Research, The Norwegian Radium Hospital, Rikshospitalet University Hospital, Oslo, Norway
    J Transl Med 6:13. 2008
    ..The aim of the present study was to investigate the potential of the MAL (T-cell differentiation protein) gene as an early epigenetic diagnostic marker for colorectal tumors...
  24. doi request reprint Fusion gene microarray reveals cancer type-specificity among fusion genes
    Marthe Løvf
    Department of Cancer Prevention, Institute for Cancer Research, Norwegian Radium Hospital, Oslo University Hospital, Oslo, Norway
    Genes Chromosomes Cancer 50:348-57. 2011
    ..We therefore find it unlikely that the assayed fusion genes are commonly present across multiple cancer types...
  25. pmc Gene expression profiles of primary colorectal carcinomas, liver metastases, and carcinomatoses
    Kristine Kleivi
    Department of Genetics, Institute for Cancer Research, Rikshospitalet Radiumhospitalet Medical Center, Oslo, Norway
    Mol Cancer 6:2. 2007
    ..Therefore, the aim of the present study is to investigate and compare the gene expression patterns of primary carcinomas (n = 18), liver metastases (n = 4), and carcinomatoses (n = 4), relative to normal samples from the large bowel...
  26. pmc Molecular characteristics of malignant ovarian germ cell tumors and comparison with testicular counterparts: implications for pathogenesis
    Sigrid Marie Kraggerud
    Department of Cancer Prevention, Institute for Cancer Research, Oslo University Hospital, N 0310 Oslo, Norway
    Endocr Rev 34:339-76. 2013
    ..This similarity combined with the process of ovary development, explain why mOGCTs present so early in life, and with greater histological complexity, than most somatic solid tumors...
  27. pmc Identification of p53 as a strong predictor of survival for patients with malignant peripheral nerve sheath tumors
    Helge R Brekke
    Department of Cancer Prevention, Institute for Cancer Research, Norwegian Radium Hospital, Rikshospitalet University Hospital, Montebello, Oslo, Norway
    Neuro Oncol 11:514-28. 2009
    ..Patients in complete remission with a primary p53-positive MPNST diagnosis may be considered in a high-risk subgroup and candidates for adjuvant treatment...
  28. ncbi request reprint The epigenome of testicular germ cell tumors
    Guro E Lind
    Department of Cancer Prevention, Rikshospitalet Radiumhospitalet Medical Centre, Montebello and Centre for Cancer Biomedicine, University of Oslo, Oslo, Norway
    APMIS 115:1147-60. 2007
    ..Aberrant DNA methylation has the potential to improve current diagnostics by noninvasive testing and might also serve as a prognostic marker for treatment response...
  29. ncbi request reprint A novel transcript, VNN1-AB, as a biomarker for colorectal cancer
    Marthe Løvf
    Department of Cancer Prevention, Institute for Cancer Research, The Norwegian Radium Hospital, Oslo University Hospital, Oslo, Norway Centre for Cancer Biomedicine, Faculty of Medicine, University of Oslo, Oslo, Norway Department of Biosciences, University of Oslo, Oslo, Norway
    Int J Cancer 135:2077-84. 2014
    ..We have identified a novel transcript of the VNN1 gene, with an organ-confined complete specificity for colorectal neoplasia. ..
  30. ncbi request reprint Chromosome 19 rearrangements in ovarian carcinomas: zinc finger genes are particularly targeted
    Marianne L Smebye
    Section for Cancer Cytogenetics, Institute for Cancer Genetics and Informatics, The Norwegian Radium Hospital, Oslo University Hospital, Oslo, Norway Centre for Cancer Biomedicine, University of Oslo, Oslo, Norway
    Genes Chromosomes Cancer 53:558-67. 2014
    ..We suggest that DNA copy number changes brought about by rearrangements of chromosome 19 contribute to ovarian carcinogenesis by leading to upregulation of ZNF223 and other zinc finger genes. © 2014 Wiley Periodicals, Inc. ..
  31. doi request reprint Survey of 548 oncogenic fusion transcripts in thyroid tumors supports the importance of the already established thyroid fusions genes
    Ricardo Celestino
    Department of Cancer Prevention, Institute for Cancer Research, Norwegian Radium Hospital, Oslo University Hospital, Oslo, Norway
    Genes Chromosomes Cancer 51:1154-64. 2012
    ..Our results reinforce the pathogenic role played by RET/PTC1, RET/PTC3, and PAX8-PPARG fusion genes in thyroid tumorigenesis...
  32. doi request reprint TPD52, a candidate gene from genomic studies, is overexpressed in testicular germ cell tumours
    Sharmini Alagaratnam
    Department of Cancer Prevention, Institute for Cancer Research, Norwegian Radium Hospital, Rikshospitalet HE, Oslo, Norway
    Mol Cell Endocrinol 306:75-80. 2009
    ..TPD52 was expressed in two-thirds of seminomas and embryonal carcinomas, and at intermediate frequencies in the more differentiated non-seminomas...
  33. pmc High frequency of fusion transcripts involving TCF7L2 in colorectal cancer: novel fusion partner and splice variants
    Torfinn Nome
    Department of Cancer Prevention, Institute for Cancer Research, Norwegian Radium Hospital Oslo University Hospital, Oslo, Norway Centre for Cancer Biomedicine, Faculty of Medicine, University of Oslo, Oslo, Norway
    PLoS ONE 9:e91264. 2014
    ..The similar fusion transcripts induced by genomic rearrangements observed in individual cancer cell lines may yet have oncogenic potential as suggested in the original study by Bass et al. ..
  34. ncbi request reprint The testicular germ cell tumour genome
    Rolf I Skotheim
    Department of Genetics, Institute for Cancer Research, The Norwegian Radium Hospital, N 0310 Oslo, Norway
    APMIS 111:136-50; discussion 50-1. 2003
    ....
  35. pmc NKX3.1 expression is lost in testicular germ cell tumors
    Rolf I Skotheim
    Department of Genetics, Institute for Cancer Research, The Norwegian Radium Hospital and University of Oslo, Oslo, Norway
    Am J Pathol 163:2149-54. 2003
    ..These results suggest that NKX3.1 is essential for normal testis function and that its loss of expression is highly associated with the invasive phenotype of testicular germ cell tumors...
  36. ncbi request reprint Lack of parental origin specificity of altered alleles at 11p15 in testicular germ cell tumors
    Sigrid M Kraggerud
    Department of Genetics, Institute for Cancer Research, The Norwegian Radium Hospital, Montebello, N 0310, Oslo, Norway
    Cancer Genet Cytogenet 147:1-8. 2003
    ..In summary, our results support the theory that a nonimprinted 11p15 tumor suppressor gene is involved in the development of a subgroup of TGCTs...
  37. doi request reprint FLI1 is a novel ETS transcription factor involved in gene fusions in prostate cancer
    Paula Paulo
    Department of Genetics, Portuguese Oncology Institute, Porto, Portugal
    Genes Chromosomes Cancer 51:240-9. 2012
    ..015). We report that FLI1 is a novel ETS transcription factor involved in gene fusions in prostate cancer and that intratumor genetic heterogeneity of ETS rearrangements can occasionally be found in index primary tumors...
  38. doi request reprint Anticipating the clinical use of prognostic gene expression-based tests for colon cancer stage II and III: is Godot finally arriving?
    Anita Sveen
    Authors Affiliations Department of Cancer Prevention, Institute for Cancer Research, The Norwegian Radium Hospital Departments of Gastrointestinal Surgery and Oncology, Oslo University Hospital and Centre for Cancer Biomedicine, Faculty of Medicine, University of Oslo, Oslo, Norway
    Clin Cancer Res 19:6669-77. 2013
    ..However, microsatellite instability is the only molecular prognostic marker recommended for clinical use...
  39. ncbi request reprint Genome profiles of familial/bilateral and sporadic testicular germ cell tumors
    Sigrid Marie Kraggerud
    Department of Genetics, Institute for Cancer Research, The Norwegian Radium Hospital, Oslo, Norway
    Genes Chromosomes Cancer 34:168-74. 2002
    ..02, respectively), whereas gain of the proximal 17q (17q11.2-21) and high-level amplification from chromosome arm 12p, and losses from 10q were associated with nonseminomas (P < 0.001, P = 0.04, and P = 0.03, respectively)...
  40. doi request reprint Reverse painting of microdissected chromosome 19 markers in ovarian carcinoma identifies a complex rearrangement map
    Francesca Micci
    Department of Medical Genetics, The Norwegian Radium Hospital, Rikshospitalet University Hospital, Montebello, Oslo, Norway
    Genes Chromosomes Cancer 48:184-93. 2009
    ..A detailed map of the rearrangements should provide clues to the positions of oncogenes and potential fusion genes important in ovarian carcinogenesis...
  41. pmc A tumor-associated mutation of FYVE-CENT prevents its interaction with Beclin 1 and interferes with cytokinesis
    Antonia P Sagona
    Centre for Cancer Biomedicine, Faculty of Medicine, University of Oslo, Oslo, Norway
    PLoS ONE 6:e17086. 2011
    ..These findings suggest a positive feedback loop for recruitment of FYVE-CENT and Beclin 1 to the intercellular bridge during cytokinesis, and reveal a novel potential tumor suppressor mechanism for Beclin 1...
  42. ncbi request reprint PtdIns(3)P controls cytokinesis through KIF13A-mediated recruitment of FYVE-CENT to the midbody
    Antonia P Sagona
    Centre for Cancer Biomedicine, Faculty of Medicine, University of Oslo, Montebello, N 0310 Oslo, Norway
    Nat Cell Biol 12:362-71. 2010
    ..These results provide a mechanism for the translocation and docking of a cytokinesis regulatory machinery at the midbody...
  43. ncbi request reprint New insights into testicular germ cell tumorigenesis from gene expression profiling
    Rolf I Skotheim
    Department of Genetics, Institute for Cancer Research, The Norwegian Radium Hospital, N 0310 Oslo, Norway
    Cancer Res 62:2359-64. 2002
    ....
  44. ncbi request reprint Alternative splicing in cancer: noise, functional, or systematic?
    Rolf I Skotheim
    Department of Cancer Prevention, Institute for Cancer Research, Rikshospitalet Radiumhospitalet Medical Center, Oslo, Norway
    Int J Biochem Cell Biol 39:1432-49. 2007
    ..These studies are expected to open an entirely new level of genetic information that is currently still poorly understood...
  45. pmc Spindle proteins are differentially expressed in the various histological subtypes of testicular germ cell tumors
    Espen Burum-Auensen
    Division of Pathology, Medical Faculty, University of Oslo, Rikshospitalet, Oslo University Hospital, Oslo, Norway
    J Carcinog 9:1. 2010
    ..The importance of these spindle proteins remains unknown in the development of TGCTs, thus we have explored the expression levels of these proteins in normal and malignant testicular tissues...