D Middleton

Summary

Affiliation: Northern Ireland

Publications

  1. ncbi Killer immunoglobulin receptor gene and allele frequencies in Caucasoid, Oriental and Black populations from different continents
    D Middleton
    Northern Ireland Regional Histocompatibility and Immunogenetics Laboratory, City Hospital, Belfast, Northern Ireland
    Tissue Antigens 71:105-13. 2008
  2. ncbi No association of KIR genes with Behcet's disease
    D Middleton
    Northern Ireland Regional Histocompatibility and Immunogenetics Laboratory, City Hospital, Belfast, UK
    Tissue Antigens 70:435-8. 2007
  3. ncbi KIR haplotype content at the allele level in 77 Northern Irish families
    D Middleton
    Northern Ireland Regional Histocompatibility and Immunogenetics Laboratory, Belfast City Hospital, Belfast, BT9 7TS, Northern Ireland, UK
    Immunogenetics 59:145-58. 2007
  4. ncbi Identification of three novel alleles of HLA-DRB1 and HLA-A in the Brazilian population
    H Abdeen
    Northern Ireland Histocompatibility and Immunogenetics Laboratory, Belfast City Hospital, Belfast, UK
    Tissue Antigens 69:607-10. 2007
  5. ncbi Characterisation of a novel HLA-A pseudogene, HLA-BEL, with significant sequence identity with a gorilla MHC class I gene
    F Williams
    Northern Ireland Regional Histocompatibility and Immunogenetics Laboratory, City Hospital, Belfast
    Tissue Antigens 54:360-9. 1999
  6. doi Investigation of killer cell immunoglobulin-like receptor gene diversity, KIR2DL1 and KIR2DS1
    A Meenagh
    Northern Ireland Regional Histocompatibility and Immunogenetics Laboratory, City Hospital, Belfast, UK
    Tissue Antigens 72:383-91. 2008
  7. ncbi Analysis of the distribution of HLA-B alleles in populations from five continents
    F Williams
    Northern Ireland Regional Histocompatibility and Immunogenetics Laboratory, City Hospital, Belfast, Northern Ireland
    Hum Immunol 62:645-50. 2001
  8. ncbi Investigation of killer cell immunoglobulin-like receptor gene diversity V. KIR3DL2
    A Meenagh
    Northern Ireland Regional Histocompatibility and Immunogenetics Laboratory, City Hospital, Belfast, UK
    Tissue Antigens 64:226-34. 2004
  9. ncbi HLA class I allele distribution of a Hong Kong Chinese population based on high-resolution PCR-SSOP typing
    D Middleton
    Northern Ireland Histocompatibility and Immunogenetics Laboratory, Blood Transfusion Building, City Hospital, Belfast, UK
    Tissue Antigens 63:555-61. 2004
  10. doi Investigation of killer cell immunoglobulin-like receptor (KIR) gene diversity: KIR2DL2, KIR2DL5 and KIR2DS5
    A Gonzalez
    Northern Ireland Regional Histocompatibility and Immunogenetics Laboratory, City Hospital, Belfast, UK
    Tissue Antigens 72:11-20. 2008

Collaborators

Detail Information

Publications48

  1. ncbi Killer immunoglobulin receptor gene and allele frequencies in Caucasoid, Oriental and Black populations from different continents
    D Middleton
    Northern Ireland Regional Histocompatibility and Immunogenetics Laboratory, City Hospital, Belfast, Northern Ireland
    Tissue Antigens 71:105-13. 2008
    ..allelefrequencies.net). In total, 5134 individuals were analysed and the populations grouped, with some exceptions, according to a geographical gradient...
  2. ncbi No association of KIR genes with Behcet's disease
    D Middleton
    Northern Ireland Regional Histocompatibility and Immunogenetics Laboratory, City Hospital, Belfast, UK
    Tissue Antigens 70:435-8. 2007
    ..Thus, no association was identified between presence or absence of any of the 14 KIR genes studied and BD. In addition, we did not find any associations of KIR with various manifestations of the disease nor with gender or age of onset...
  3. ncbi KIR haplotype content at the allele level in 77 Northern Irish families
    D Middleton
    Northern Ireland Regional Histocompatibility and Immunogenetics Laboratory, Belfast City Hospital, Belfast, BT9 7TS, Northern Ireland, UK
    Immunogenetics 59:145-58. 2007
    ..1%, 9.7% and 12.6% for KIR2DL4, KIR3DL2 and KIR3DL1 genes, respectively, but 62.6% and 53% for KIR2DL3 and KIR2DS4 genes, respectively, despite the fact that no one allele dominated the frequency in any of these genes...
  4. ncbi Identification of three novel alleles of HLA-DRB1 and HLA-A in the Brazilian population
    H Abdeen
    Northern Ireland Histocompatibility and Immunogenetics Laboratory, Belfast City Hospital, Belfast, UK
    Tissue Antigens 69:607-10. 2007
    ..These three novel alleles may have originated from other HLA alleles by gene conversion. However, it is also possible that HLA-A*2471 has evolved from one of the alleles of the HLA-A*2402 group through a point mutation...
  5. ncbi Characterisation of a novel HLA-A pseudogene, HLA-BEL, with significant sequence identity with a gorilla MHC class I gene
    F Williams
    Northern Ireland Regional Histocompatibility and Immunogenetics Laboratory, City Hospital, Belfast
    Tissue Antigens 54:360-9. 1999
    ..Its unique gene structure gives a possible insight into the evolutionary pathway that created HLA class I genes...
  6. doi Investigation of killer cell immunoglobulin-like receptor gene diversity, KIR2DL1 and KIR2DS1
    A Meenagh
    Northern Ireland Regional Histocompatibility and Immunogenetics Laboratory, City Hospital, Belfast, UK
    Tissue Antigens 72:383-91. 2008
    ..Results also indicated an inherited KIR2DL1/2DS1 splice variant present in a haplotype with several core loci absent, in two families...
  7. ncbi Analysis of the distribution of HLA-B alleles in populations from five continents
    F Williams
    Northern Ireland Regional Histocompatibility and Immunogenetics Laboratory, City Hospital, Belfast, Northern Ireland
    Hum Immunol 62:645-50. 2001
    ..HLA-B allele frequencies and HLA-A/B two locus haplotype frequencies were compiled for each population...
  8. ncbi Investigation of killer cell immunoglobulin-like receptor gene diversity V. KIR3DL2
    A Meenagh
    Northern Ireland Regional Histocompatibility and Immunogenetics Laboratory, City Hospital, Belfast, UK
    Tissue Antigens 64:226-34. 2004
    ..A high level of KIR3DL2 allelic polymorphism has been identified...
  9. ncbi HLA class I allele distribution of a Hong Kong Chinese population based on high-resolution PCR-SSOP typing
    D Middleton
    Northern Ireland Histocompatibility and Immunogenetics Laboratory, Blood Transfusion Building, City Hospital, Belfast, UK
    Tissue Antigens 63:555-61. 2004
    ..In addition, knowing which alleles do/do not occur in this population will aid in the distinction of ambiguities which result from the use of many of the typing kits available...
  10. doi Investigation of killer cell immunoglobulin-like receptor (KIR) gene diversity: KIR2DL2, KIR2DL5 and KIR2DS5
    A Gonzalez
    Northern Ireland Regional Histocompatibility and Immunogenetics Laboratory, City Hospital, Belfast, UK
    Tissue Antigens 72:11-20. 2008
    ..This information complements published reports by our laboratory for allele-level typing of other KIR members, totaling 12 of the 17 known genes. These methods are allowing us to characterize KIR haplotypes in our population...
  11. ncbi Characterization of HLA-B*3921 and confirmation of HLA-B*4415, two variant HLA-B alleles identified in the Omani population
    F Williams
    Northern Ireland Regional Histocompatibility and Immunogenetics Laboratory, City Hospital, Belfast
    Tissue Antigens 56:376-9. 2000
    ..In addition we confirm the existence of HLA-B*4415, an allele closely related to HLA-B*4501 differing only at the Bw4/Bw6 epitope...
  12. ncbi Aberrant splicing of intron 1 creates a novel null HLA-B*1501 allele
    M D Curran
    Northern Ireland Histocompatibility and Immunogenetics Laboratory, City Hospital, Belfast, UK
    Tissue Antigens 53:244-52. 1999
    ..Our data predicts that the HLA-B*1501 null allele would express a small truncated protein containing the signal sequence fused to an ORF within intron 1 and terminating (out of frame) just within exon 2...
  13. ncbi Analysis of KIR gene frequencies in HLA class I characterised bladder, colorectal and laryngeal tumours
    D Middleton
    Northern Ireland Histocompatibility and Immunogenetics Laboratory, Blood Transfusion Building, City Hospital, Belfast, Northern Ireland, UK
    Tissue Antigens 69:220-6. 2007
    ..Finally, in the group of colorectal carcinomas, there was an overall significant difference in the frequencies of C group heterozygosity and homozygosity with HLA alterations on the tumour...
  14. ncbi Investigation of killer cell immunoglobulin-like receptor gene diversity III. KIR2DL3
    L Keaney
    Northern Ireland Regional Histocompatibility and Immunogenetics Laboratory, City Hospital, Belfast, Northern Ireland
    Tissue Antigens 64:188-94. 2004
    ..In addition, cell line DNA and Centre d'Etude du Humaine (CEPH) families, both from the 13th International Histocompatibility Workshop have been investigated, establishing control data for this gene...
  15. ncbi No association in frequency of KIR receptors in patients with rheumatoid arthritis from Northern Ireland
    D Middleton
    Northern Ireland Histocompatibility and Immunogenetics Laboratory, City Hospital, Belfast, Northern Ireland
    Tissue Antigens 69:577-82. 2007
    ..Furthermore, no differences were found when the patients were sub-divided according to gender or shared epitope and when the presence of the human leucocyte antigen ligand was taken into account...
  16. doi No association of KIR3DL1 or KIR3DS1 or their alleles with ankylosing spondylitis
    J McCappin
    Northern Ireland Regional Histocompatibility and Immunogenetics Laboratory, Belfast City Hospital, Belfast, UK
    Tissue Antigens 75:68-73. 2010
    ..We found no association in frequencies of the alleles of these genes in AS. In addition, no differences were found when the patients and controls were differentiated by gender...
  17. ncbi Characterisation of a new HLA-B allele, HLA-B*0720, identified in the Cuban population
    F Williams
    Northern Ireland Regional Histocompatibility and Immunogenetics Laboratory, City Hospital, Belfast, Northern Ireland
    Tissue Antigens 57:80-2. 2001
    ..To date, 286 HLA-B alleles have been identified and characterised. We describe a new HLA-B*07 allele present in a Cuban Caucasoid individual, which has been officially named HLA-B*0720...
  18. ncbi Modification of an HLA-B PCR-SSOP typing system leading to improved allele determination
    D Middleton
    Northern Ireland Tissue Typing Laboratory, City Hospital, Belfast, Northern Ireland, United Kingdom
    Tissue Antigens 45:232-6. 1995
    ..When the method was applied to the typing of 66 consecutive cadaveric donors there were three donors whose type differed from the serological type...
  19. ncbi Analysis of the distribution of HLA-A alleles in populations from five continents
    D Middleton
    Northern Ireland Regional Histocompatibility and Immunogenetics Laboratory, City Hospital, Belfast, Northern Ireland, UK
    Hum Immunol 61:1048-52. 2000
    ..It would appear by their presence in all populations studied, either rural or indigenous, that certain alleles are very important in pathogen peptide presentation...
  20. ncbi A HLA-Cw*03 allele, Cw*0322Q with limited or no expression
    D Middleton
    Northern Ireland Histocompatiblity and Immunogenetics Laboratory, Blood Transfusion Building, Belfast City Hospital, Belfast, BT9 7TS, Northern Ireland
    Tissue Antigens 67:343-5. 2006
  21. ncbi A common KIR2DS4 deletion variant in the human that predicts a soluble KIR molecule analogous to the KIR1D molecule observed in the rhesus monkey
    L D Maxwell
    Northern Ireland Regional Histocompatibility and Immunogenetics Laboratory, City Hospital, Belfast, Northern Ireland
    Tissue Antigens 60:254-8. 2002
    ..Parallels with a recent study in the rhesus monkey highlighting access to the same open reading frame as the deletion variant, also predicting a soluble KIR molecule, are drawn...
  22. ncbi Identification of a new HLA-B*55 allele, HLA-B*5503
    F Williams
    Northern Ireland Tissue Typing Laboratory, City Hospital, Belfast, United Kingdom
    Tissue Antigens 48:598-9. 1996
  23. ncbi Mitochondrial DNA polymorphism: its role in longevity of the Irish population
    O A Ross
    Northern Ireland Regional Histocompatibility and Immunogenetics Laboratory, Belfast City Hospital, Blood Transfusing Building, Belfast NI BT9 7TS, UK
    Exp Gerontol 36:1161-78. 2001
    ..164...
  24. ncbi A novel HLA-B allele, HLA-B*1803
    M D Curran
    Northern Ireland Tissue Typing Laboratory, City Hospital, Belfast, Northern Ireland, United Kingdom
    Tissue Antigens 48:708-10. 1996
  25. ncbi Development of a PCR-SSOP approach capable of defining the natural killer cell inhibitory receptor (KIR) gene sequence repertoires
    K A Crum
    Northern Ireland Regional Histocompatibility and Immunogenetics Laboratory, City Hospital, Belfast
    Tissue Antigens 56:313-26. 2000
    ..The segregation analysis concluded that at least 3 distinct gene loci encode 2DL1-4 and at least 4 gene loci encode the non-inhibitory KIR2DS1-2DS5. In the case of 3DL1-2 and 3DS1, our data suggests 3 gene loci, one for each subfamily...
  26. ncbi KIR allele and gene polymorphism group (KAG)
    D Middleton
    Northern Ireland Histocompatibility and Immunogenetics Laboratory, City Hospital, Belfast, BT9 7TS Northern Ireland
    Mol Immunol 42:455-7. 2005
    ..Discussion took place on nomenclature issues of the KIR genes and their alleles, and a website has been developed to collect frequencies of KIR genes and alleles in various diverse populations...
  27. ncbi Identification of two novel alleles HLA-B*3569 and -B*4450 and confirmation of HLA-A*2631 in the Brazilian population
    H Abdeen
    Northern Ireland Histocompatibility and Immunogenetics Laboratory, Belfast City Hospital, Belfast, UK
    Tissue Antigens 69:273-6. 2007
    ..It appears that HLA-B*3569 arose from the analogous HLA-B*3510 through a point mutation. However, HLA-B*4450 may have arisen from HLA-B*440301 and -B*4425 by gene conversion...
  28. ncbi 14th International HLA and Immunogenetics Workshop: report on KIR receptors and their applications
    D Middleton
    Northern Ireland Histocompatibility and Immunogenetics Laboratory, City Hospital, Belfast, Northern Ireland
    Tissue Antigens 69:85-7. 2007
    ..To introduce this work, we provide an introduction to KIR receptors and an outline of previous applications of KIR receptor typing prior to the Workshop...
  29. ncbi Characterisation of a new HLA-B allele, HLA-B*0724
    D Middleton
    Northern Ireland Regional Histocompatibility and Immunogenetics Laboratory, City Hospital, Belfast, Northern Ireland, UK
    Tissue Antigens 57:471-3. 2001
  30. ncbi New allele frequency database: http://www.allelefrequencies.net
    D Middleton
    Northern Ireland Histocompatibility and Immunogenetics Laboratory, Belfast, Northern Ireland
    Tissue Antigens 61:403-7. 2003
    ..Data for HLA alleles has been added but this will be extended to include frequency data of polymorphisms in other immunogenetic regions, e.g. cytokines, KIR receptors, MIC...
  31. ncbi Genetic, morphometric and immunohistochemical markers of latent coeliac disease
    S D Johnston
    Department of Medicine, Queen s University, Belfast, Northern Ireland, UK
    Eur J Gastroenterol Hepatol 11:1283-8. 1999
    ....
  32. ncbi A new HLA-B51 allele, B*5107 in RCE55 detected and characterized by PCR-SSOP, cloning and nucleotide sequence determination
    M D Curran
    Northern Ireland Tissue Typing Laboratory, City Hospital, Belfast
    Tissue Antigens 48:228-30. 1996
  33. ncbi KIR genes polymorphism in Argentinean Caucasoid and Amerindian populations
    A C Flores
    Laboratorio de Inmunogenética, Hospital de Clinicas, Facultad de Medicina, UBA, Buenos Aires, Argentina
    Tissue Antigens 69:568-76. 2007
    ..The Amerindian populations showed several similarities to Asian but not to Caucasoid populations with regard to the frequency of KIR2DS3, full-length KIR2DS4 gene and KIR2DL4 alleles...
  34. ncbi HLA class I and II polymorphisms in Azores show different settlements in Oriental and Central islands
    H Spinola
    Human Genetics Laboratory, University of Madeira, Funchal, Portugal
    Tissue Antigens 66:217-30. 2005
    ..The Central group of islands on the contrary clearly shows an influence of north Europeans (most probably derived from a well-documented Flemish settlement), with much less affinity to mainland Portugal...
  35. ncbi Mismatched human leukocyte antigen alleles protect against heterosexual HIV transmission
    S F Lockett
    Centre for HIV Research, Institute of Cell, Animal and Population Biology, University of Edinburgh, Edinburgh, Scotland
    J Acquir Immune Defic Syndr 27:277-80. 2001
    ..Analysis of the frequencies of specific alleles at the A, B, and DR loci revealed a significantly higher frequency of HLA DR5 among exposed uninfected individuals, relative to population controls...
  36. ncbi Frequencies of HLA-A, HLA-B, HLA-DR, and HLA-DQ phenotypes in the United Arab Emirates population
    V Valluri
    Immunology and Specialized testing, Mafrag Hospital, Abu Dhabi, UAE
    Tissue Antigens 66:107-13. 2005
    ..It also sheds light on the similarities between the UAE Emiratis, AGP Arabs, Iranians, and Asians, specially the predominance of DR2 of the class II antigens...
  37. ncbi HLA genes in Portugal inferred from sequence-based typing: in the crossroad between Europe and Africa
    H Spinola
    Human Genetics Laboratory, University of Madeira, Campus of Penteada, Funchal, Portugal
    Tissue Antigens 66:26-36. 2005
    ..North and South Portugal show more similarity to North Africans in opposition to Centre which appears closer to Europeans...
  38. ncbi Association of functional heme oxygenase-1 gene promoter polymorphism with renal transplantation outcomes
    A E Courtney
    Nephrology Research Group, Queen s University of Belfast, and Regional Nephrology Unit, Belfast City Hospital, Belfast BT9 7AB, UK
    Am J Transplant 7:908-13. 2007
    ..There is no evidence of a protective effect for the S-allele of the HO-1 gene promoter polymorphism on graft or recipient survival in clinical renal transplantation...
  39. ncbi HLA-A *2607: sequence of a novel A*26 subtype predicted by DNA typing which shares the MA2.1 epitope with A*02, B*57 and B*58
    K L Arnett
    Department of Structural Biology, Stanford University, California, USA
    Tissue Antigens 47:422-5. 1996
  40. ncbi The influence of transforming growth factor-beta1 gene polymorphisms on the severity of gingival overgrowth associated with concomitant use of cyclosporin A and a calcium channel blocker
    G J Linden
    Division of Restorative Dentistry, School of Dentistry, Queen s University of Belfast, UK
    J Periodontol 72:808-14. 2001
    ....
  41. ncbi Pathogenesis studies with Australian bat lyssavirus in grey-headed flying foxes (Pteropus poliocephalus)
    K A McColl
    CSIRO Livestock Industries, Australian Animal Health Laboratory, PO Bag 24, Geelong, Victoria 3220
    Aust Vet J 80:636-41. 2002
    ..To examine the susceptibility of the grey-headed flying fox (Pteropus poliocephalus) to Australian bat lyssavirus (ABL), and to provide preliminary observations on the pathogenesis of the disease in flying foxes...
  42. ncbi Killer-cell immunoglobulin-like receptor (KIR) nomenclature report, 2002
    S G E Marsh
    Anthony Nolan Research Institute, Royal Free Hospital, Pond Street, Hampstead, London NW3 2QG, UK
    Eur J Immunogenet 30:229-34. 2003
  43. ncbi Killer-cell immunoglobulin-like receptor (KIR) nomenclature report, 2002
    S G E Marsh
    Anthony Nolan Research Institute, Royal Free Hospital, London, UK
    Tissue Antigens 62:79-86. 2003
  44. ncbi Genetic polymorphism of IL-12 p40 gene in immune-mediated disease
    M A Hall
    Department of Rheumatology, Division of Medicine, 5th Floor Thomas Guy House, Guy s, King s and St Thomas Hospitals School of Medicine, King s College, Guy s Hospital Campus, London SE1 9RT, UK
    Genes Immun 1:219-24. 2000
    ..02 95% CI 0.95-4.3). This new genetic marker could be useful in anthropological studies and should be investigated in other autoimmune, allergic, inflammatory and infectious diseases...
  45. ncbi Distribution of HLA alleles in Portugal and Cabo Verde. Relationships with the slave trade route
    H Spinola
    Human Genetics Laboratory, Centre of Macaronesian Studies, University of Madeira, Campus of Penteada, 9000 Funchal, Portugal
    Ann Hum Genet 66:285-96. 2002
    ..A PCA of the populations studied, plus other relevant ones, clearly shows gene heterogeneity in mainland Portugal as well as the differences and relationships between these populations and Madeira and Cabo Verde...
  46. ncbi KIR receptors and HLA-C in the maintenance of pregnancy
    A C Flores
    Laboratorio de Inmunogenética, Hospital de Clinicas, Buenos Aires, Argentina
    Tissue Antigens 69:112-3. 2007
    ..The present study might support that in RSA patients, the balance between inhibitory and activating receptors present in natural killer cells is inclined toward an activating state that may contribute to pregnancy loss...
  47. ncbi Increased frequencies of activating natural killer receptors are associated with liver injury in individuals who do not eliminate hepatitis C virus
    N Paladino
    División Inmunogenética, Hospital de Clinicas Jose de San Martin, Facultad de Medicina, Universidad de Bueno Aires, Buenos Aires, Argentina
    Tissue Antigens 69:109-11. 2007
    ..016). We conclude that higher natural killer cytotoxicity might be associated with a worse progression of the HCV infection...
  48. ncbi Preliminary analysis of a KIR haplotype estimation algorithm: a simulation study
    P A Gourraud
    INSERM U558, Department of Epidemiology, Faculty of Medicine, France
    Tissue Antigens 69:96-100. 2007
    ..Large sample size studies are required using phase-known data and/or simulations. It would allow a genotype-based approach to explore the KIR gene haplotype diversity. The haplotype frequencies may be used to compare populations...