Nomikos M, Theodoridou M, Elgmati K, Parthimos D, Calver B, Buntwal L, et al
. Human PLC? exhibits superior fertilization potency over mouse PLC? in triggering the Ca(2+) oscillations required for mammalian oocyte activation. Mol Hum Reprod. 2014;20:489-98 pubmed publisher
..Our empirical observations are compatible with a basic mathematical model for the Ca(2+) dependence of generating cytoplasmic Ca(2+) oscillations in mammalian oocytes by sperm PLC?. ..
Nomikos M, Stamatiadis P, Sanders J, Beck K, Calver B, Buntwal L, et al
. Male infertility-linked point mutation reveals a vital binding role for the C2 domain of sperm PLC?. Biochem J. 2017;474:1003-1016 pubmed publisher
..Our findings highlight the importance of PLC? at fertilisation and the vital role of the C2 domain in PLC? function, possibly due to its novel binding characteristics. ..
Da as S, Fakhro K, Thanassoulas A, Krishnamoorthy N, Saleh A, Calver B, et al
. Hypertrophic cardiomyopathy-linked variants of cardiac myosin binding protein C3 display altered molecular properties and actin interaction. Biochem J. 2018;: pubmed publisher
Nomikos M, Kashir J, Swann K, Lai F. Sperm PLC?: from structure to Ca2+ oscillations, egg activation and therapeutic potential. FEBS Lett. 2013;587:3609-16 pubmed publisher
..PLC? appears to be unique in its ability to target PIP2 that is present on intracellular vesicles. We also discuss evidence that PLC? may be a significant factor in human fertility with potential therapeutic capacity. ..
Nomikos M, Sanders J, Theodoridou M, Kashir J, Matthews E, Nounesis G, et al
. Sperm-specific post-acrosomal WW-domain binding protein (PAWP) does not cause Ca2+ release in mouse oocytes. Mol Hum Reprod. 2014;20:938-47 pubmed publisher
..These data suggest that sperm-derived PAWP is not involved in triggering Ca(2+) oscillations at fertilization in mammalian oocytes. ..
Vassilakopoulou V, Calver B, Thanassoulas A, Beck K, Hu H, Buntwal L, et al
. Distinctive malfunctions of calmodulin mutations associated with heart RyR2-mediated arrhythmic disease. Biochim Biophys Acta. 2015;1850:2168-76 pubmed publisher
..Our findings suggest that the clinical presentation of CPVT or LQTS associated with these five CaM mutations may involve both altered intrinsic Ca(2+)-binding as well as defective interaction with RyR2. ..
Nomikos M, Kashir J, Lai F. The role and mechanism of action of sperm PLC-zeta in mammalian fertilisation. Biochem J. 2017;474:3659-3673 pubmed publisher
..We also postulate the significant potential diagnostic and therapeutic capacity of PLC? in alleviating certain types of male infertility...
Nomikos M, Elgmati K, Theodoridou M, Georgilis A, Gonzalez Garcia J, Nounesis G, et al
. Novel regulation of PLC? activity via its XY-linker. Biochem J. 2011;438:427-32 pubmed publisher
Nomikos M, Sanders J, Parthimos D, Buntwal L, Calver B, Stamatiadis P, et al
. Essential Role of the EF-hand Domain in Targeting Sperm Phospholipase CÎ¶ to Membrane Phosphatidylinositol 4,5-Bisphosphate (PIP2). J Biol Chem. 2015;290:29519-30 pubmed publisher