John G Lewis

Summary

Country: New Zealand

Publications

  1. ncbi request reprint An enzyme-linked immunosorbent assay for corticosteroid-binding globulin using monoclonal and polyclonal antibodies: decline in CBG following synthetic ACTH
    John G Lewis
    Steroid and Immunobiochemistry Laboratory, Canterbury Health Laboratories, PO Box 151, Christchurch, New Zealand
    Clin Chim Acta 328:121-8. 2003
  2. ncbi request reprint Plasma variation of corticosteroid-binding globulin and sex hormone-binding globulin
    J G Lewis
    Steroid and Immunobiochemistry Laboratory, Canterbury Health Laboratories, Christchurch, New Zealand
    Horm Metab Res 38:241-5. 2006
  3. doi request reprint Plasma retinol-binding protein is unlikely to be a useful marker of insulin resistance
    John G Lewis
    Steroid and Immunobiochemistry Laboratory, Canterbury Health Laboratories, Christchurch 8001, New Zealand
    Diabetes Res Clin Pract 80:e13-5. 2008
  4. doi request reprint Corticosteroid-binding globulin reactive centre loop antibodies recognise only the intact natured protein: elastase cleaved and uncleaved CBG may coexist in circulation
    John G Lewis
    Steroid and Immunobiochemistry Laboratory, Canterbury Health Laboratories, PO Box 151, Christchurch, New Zealand
    J Steroid Biochem Mol Biol 127:289-94. 2011
  5. doi request reprint Intact or "active" corticosteroid-binding globulin (CBG) and total CBG in plasma: determination by parallel ELISAs using monoclonal antibodies
    John G Lewis
    Steroid and Immunobiochemistry Laboratory, Canterbury Health Laboratories, Christchurch, New Zealand
    Clin Chim Acta 416:26-30. 2013
  6. doi request reprint Corticosteroid-binding globulin, cortisol, free cortisol, and sex hormone-binding globulin responses following oral glucose challenge in spinal cord-injured and able-bodied men
    J G Lewis
    Steroid and Immunobiochemistry Laboratory, Canterbury Health Laboratories, Christchurch, New Zealand
    Horm Metab Res 42:882-6. 2010
  7. ncbi request reprint An ELISA for plasma retinol-binding protein using monoclonal and polyclonal antibodies: plasma variation in normal and insulin resistant subjects
    John G Lewis
    Steroid and Immunobiochemistry Laboratory, Canterbury Health Laboratories, Christchurch, New Zealand
    Clin Biochem 40:828-34. 2007
  8. ncbi request reprint Circulating levels of isoflavones and markers of 5alpha-reductase activity are higher in Japanese compared with New Zealand males: what is the role of circulating steroids in prostate disease?
    J G Lewis
    Steroid and Immunobiochemistry Laboratory, Canterbury Health Laboratories, P O Box 151, Christchurch 8001, New Zealand
    Steroids 70:974-9. 2005
  9. ncbi request reprint Plasma retinol-binding protein is not a marker of insulin resistance in overweight subjects: a three year longitudinal study
    John G Lewis
    Steroid and Immunobiochemistry Laboratory, Canterbury Health Laboratories, P O Box 151, Christchurch, New Zealand
    Clin Biochem 41:1034-8. 2008
  10. ncbi request reprint Plasma free cortisol fraction reflects levels of functioning corticosteroid-binding globulin
    John G Lewis
    Steroid and Immunobiochemistry Laboratory, Canterbury Health Laboratories, PO Box 151 Christchurch, New Zealand
    Clin Chim Acta 359:189-94. 2005

Collaborators

Detail Information

Publications33

  1. ncbi request reprint An enzyme-linked immunosorbent assay for corticosteroid-binding globulin using monoclonal and polyclonal antibodies: decline in CBG following synthetic ACTH
    John G Lewis
    Steroid and Immunobiochemistry Laboratory, Canterbury Health Laboratories, PO Box 151, Christchurch, New Zealand
    Clin Chim Acta 328:121-8. 2003
    ..We have developed and validated an enzyme-linked immunosorbent assay (ELISA) for CBG and together with plasma cortisol calculated the "free cortisol index" as an additional parameter for assessing adrenal function...
  2. ncbi request reprint Plasma variation of corticosteroid-binding globulin and sex hormone-binding globulin
    J G Lewis
    Steroid and Immunobiochemistry Laboratory, Canterbury Health Laboratories, Christchurch, New Zealand
    Horm Metab Res 38:241-5. 2006
    ..The consequence of lower levels of CBG in these obese male subjects is reflected by higher levels of circulating free cortisol, potentially offering a more favourable environment for adipogenesis...
  3. doi request reprint Plasma retinol-binding protein is unlikely to be a useful marker of insulin resistance
    John G Lewis
    Steroid and Immunobiochemistry Laboratory, Canterbury Health Laboratories, Christchurch 8001, New Zealand
    Diabetes Res Clin Pract 80:e13-5. 2008
    ..We confirm, using the largest study cohort to date, that plasma RBP is unlikely to be a useful marker of insulin resistance...
  4. doi request reprint Corticosteroid-binding globulin reactive centre loop antibodies recognise only the intact natured protein: elastase cleaved and uncleaved CBG may coexist in circulation
    John G Lewis
    Steroid and Immunobiochemistry Laboratory, Canterbury Health Laboratories, PO Box 151, Christchurch, New Zealand
    J Steroid Biochem Mol Biol 127:289-94. 2011
    ..This antibody could be used for determining the time course of intact CBG in various relevant patient cohorts and for structure/function studies on the biology of human CBG...
  5. doi request reprint Intact or "active" corticosteroid-binding globulin (CBG) and total CBG in plasma: determination by parallel ELISAs using monoclonal antibodies
    John G Lewis
    Steroid and Immunobiochemistry Laboratory, Canterbury Health Laboratories, Christchurch, New Zealand
    Clin Chim Acta 416:26-30. 2013
    ..Gross differences in total and intact CBG levels may not be due to differences in N-glycosylation affecting antibody binding as CBG levels are unaffected by PNGase F treatment...
  6. doi request reprint Corticosteroid-binding globulin, cortisol, free cortisol, and sex hormone-binding globulin responses following oral glucose challenge in spinal cord-injured and able-bodied men
    J G Lewis
    Steroid and Immunobiochemistry Laboratory, Canterbury Health Laboratories, Christchurch, New Zealand
    Horm Metab Res 42:882-6. 2010
    ..There were no significant changes in plasma sex hormone-binding globulin in either group...
  7. ncbi request reprint An ELISA for plasma retinol-binding protein using monoclonal and polyclonal antibodies: plasma variation in normal and insulin resistant subjects
    John G Lewis
    Steroid and Immunobiochemistry Laboratory, Canterbury Health Laboratories, Christchurch, New Zealand
    Clin Biochem 40:828-34. 2007
    ..We examined this in normal subjects and compared plasma levels and variability in lean subjects and subjects with the metabolic syndrome...
  8. ncbi request reprint Circulating levels of isoflavones and markers of 5alpha-reductase activity are higher in Japanese compared with New Zealand males: what is the role of circulating steroids in prostate disease?
    J G Lewis
    Steroid and Immunobiochemistry Laboratory, Canterbury Health Laboratories, P O Box 151, Christchurch 8001, New Zealand
    Steroids 70:974-9. 2005
    ..Significantly the positive association between isoflavones levels and 5alpha-steroids is counter-intuitive to isoflavone intake offering prostate protection, unless this is postulated to occur through other mechanisms...
  9. ncbi request reprint Plasma retinol-binding protein is not a marker of insulin resistance in overweight subjects: a three year longitudinal study
    John G Lewis
    Steroid and Immunobiochemistry Laboratory, Canterbury Health Laboratories, P O Box 151, Christchurch, New Zealand
    Clin Biochem 41:1034-8. 2008
    ..This longitudinal study investigated whether or not plasma retinol-binding protein (RBP), recently referred to as RBP4, was a marker of insulin resistance in overweight subjects...
  10. ncbi request reprint Plasma free cortisol fraction reflects levels of functioning corticosteroid-binding globulin
    John G Lewis
    Steroid and Immunobiochemistry Laboratory, Canterbury Health Laboratories, PO Box 151 Christchurch, New Zealand
    Clin Chim Acta 359:189-94. 2005
    ..However little is known about the distribution of free cortisol in plasma where CBG is inactivated or in congenital CBG deficiency...
  11. ncbi request reprint Plasma sex hormone-binding globulin rather than corticosteroid-binding globulin is a marker of insulin resistance in obese adult males
    J G Lewis
    Steroid and Immunobiochemistry Laboratory, Canterbury Health Laboratories, Christchurch, New Zealand
    Diabetes Obes Metab 6:259-63. 2004
    ..The aim of this study was to test the hypothesis that these steroid-binding proteins are markers of insulin resistance and obesity in adult patients with the metabolic syndrome...
  12. doi request reprint Plasma levels of sex hormone-binding globulin, corticosteroid-binding globulin and cortisol in overweight subjects who develop impaired fasting glucose: a 3-year prospective study
    J G Lewis
    Steroid and Immunobiochemistry Laboratory, Canterbury Health Laboratories, Christchurch, New Zealand
    Horm Metab Res 41:255-9. 2009
    ....
  13. ncbi request reprint Caution on the use of saliva measurements to monitor absorption of progesterone from transdermal creams in postmenopausal women
    John G Lewis
    Steroid and Immunobiochemistry Laboratory, Canterbury Health Laboratories, P O Box 151, Christchurch, New Zealand
    Maturitas 41:1-6. 2002
    ..To determine the levels of progesterone in plasma, red cells and saliva as well as pregnanediol-3-glucuronide excretion in postmenopausal women using transdermal progesterone creams...
  14. ncbi request reprint ZZ polyester beads: an efficient and simple method for purifying IgG from mouse hybridoma supernatants
    John G Lewis
    Steroid and Immunobiochemistry Laboratory, Canterbury Health Laboratories, Christchurch, New Zealand
    J Immunol Methods 346:71-4. 2009
    ..As a negative control we used culture supernatant from an IgM secreting hybridoma and showed that it did not bind to ZZ polyester beads...
  15. ncbi request reprint Is late-night salivary cortisol a better screening test for possible cortisol excess than standard screening tests in obese patients with Type 2 diabetes?
    Elna Ellis
    Christchurch Hospital, Christchurch, New Zealand
    N Z Med J 125:47-58. 2012
    ....
  16. ncbi request reprint An enzyme-linked immunosorbent assay (ELISA) for methylphenidate (Ritalin ) in urine
    Mark G Lewis
    Canterbury Health Laboratories, Christchurch, New Zealand
    J Anal Toxicol 27:342-5. 2003
    ..This method is unaffected by drugs of abuse and is suitable for routine use in the toxicology laboratory...
  17. ncbi request reprint A case of low cortisol-binding globulin: use of plasma free cortisol in interpretation of hypothalamic-pituitary-adrenal axis tests
    James S Davidson
    Department of Chemical Pathology, LabPlus, Auckland City Hospital, Auckland, New Zealand
    Ann Clin Biochem 43:237-9. 2006
    ..Measurement of CBG concentration and calculation of free cortisol or free cortisol index can help avoid false interpretations of dynamic tests of the HPA axis based on plasma total cortisol...
  18. ncbi request reprint Monoclonal antibodies to human sex hormone-binding globulin (SHBG): characterization and use in a simple enzyme-linked immunosorbent assay (ELISA) of SHBG in plasma
    J G Lewis
    Steroid and Immunobiochemistry Laboratory, Canterbury Health Laboratories, Christchurch, New Zealand
    Steroids 64:259-65. 1999
    ..The monoclonal antibody-based ELISA shows excellent performance characteristics and is unaffected by added testosterone or estradiol...
  19. ncbi request reprint Two cases of adrenal suppression following a Chinese herbal remedy: a cause for concern?
    C M Florkowski
    Canterbury Health Laboratories, Christchurch Hospital
    N Z Med J 115:223-4. 2002
  20. doi request reprint Comparison of indices of insulin resistance with metabolic syndrome classifications to predict the development of impaired fasting glucose in overweight and obese subjects: a 3-year prospective study
    B I Shand
    Lipid and Diabetes Research Group, Christchurch Hospital, Christchurch, New Zealand
    Int J Obes (Lond) 33:1274-9. 2009
    ..To compare the ability of biochemical indices of insulin resistance (IR) with metabolic syndrome (MetS) classifications to predict changes in blood glucose control over a 3-year period in overweight and obese subjects...
  21. doi request reprint Immune reactivity of four monoclonal and two polyclonal antibodies raised against recombinant pneumococcal surface adhesin A
    Anja M Werno
    Microbiology Unit, Canterbury Health Laboratories, Christchurch, New Zealand
    Hybridoma (Larchmt) 31:168-75. 2012
    ..The specificity of the four MAbs appears to be high as they only recognized S. pneumoniae and S. pseudopneumoniae. However, further testing of closely related streptococcal species is necessary to confirm this finding...
  22. doi request reprint Plasma sex hormone-binding globulin, corticosteroid-binding globulin, cortisol, and free cortisol levels in outpatients attending a lipid disorders clinic: a cross-sectional study of 1137 subjects
    J G Lewis
    Department of Clinical Biochemistry, Canterbury Health Laboratories, Christchurch, New Zealand
    Horm Metab Res 42:274-9. 2010
    ..However, the aetiological role of CBG and cortisol in insulin resistance is uncertain, although in males, cortisol and CBG could be subtly related to the degree of insulin resistance...
  23. ncbi request reprint Plasma betaine concentrations correlate with plasma cortisol but not with C-reactive protein in an elderly population
    Michael Lever
    Clinical Biochemistry Unit, Canterbury Health Laboratories, Christchurch, New Zealand
    Clin Chem Lab Med 50:1635-40. 2012
    ..We aimed to compare plasma cortisol (a stress marker) and C-reactive protein (an inflammation marker) as statistical predictors of plasma betaine concentrations...
  24. doi request reprint A patient with pseudo-Addison's disease and falsely elevated thyroxine due to interference in serum cortisol and free thyroxine immunoassays by two different mechanisms
    Mohamed Saleem
    Canterbury Health Laboratories, Christchurch, New Zealand
    Ann Clin Biochem 46:172-5. 2009
    ..Good clinician-laboratory interface and laboratory work-up of patients with results that are discordant from the clinical findings can reduce unnecessary investigation and inappropriate treatment...
  25. ncbi request reprint The effect of isoflavone extract ingestion, as Trinovin, on plasma steroids in normal men
    John G Lewis
    Steroid and Immunobiochemistry Laboratory, Canterbury Health Laboratories, Christchurch, New Zealand
    Steroids 67:25-9. 2002
    ..In contrast the increase in dihydrotestosterone plasma levels could be seen as possibly detrimental...
  26. doi request reprint The reactive centre loop of corticosteroid-binding globulin (CBG) is a protease target for cortisol release
    John G Lewis
    Steroid and Immunobiochemistry Laboratory, Canterbury Health Laboratories, Christchurch, New Zealand Electronic address
    Mol Cell Endocrinol 384:96-101. 2014
    ..These findings demonstrate a new mechanism for cortisol release from its binding globulin. ..
  27. ncbi request reprint Reduced maternal corticosteroid-binding globulin and cortisol levels in pre-eclampsia and gamete recipient pregnancies
    Jui T Ho
    Hanson Institute, University of Adelaide, and Endocrine and Metabolic Unit of the Royal Adelaide Hospital, Adelaide, South Australia, Australia
    Clin Endocrinol (Oxf) 66:869-77. 2007
    ..To measure and contrast maternal cortisol and corticosteroid-binding globulin (CBG) levels in pregnancies with normal outcomes, pre-eclampsia, intrauterine growth restriction (IUGR) and in gamete recipients...
  28. ncbi request reprint Hormonal effects on the secretion and glycoform profile of corticosteroid-binding globulin
    Robin Mihrshahi
    Department of Biological Sciences, Macquarie University, Sydney, NSW 2109, Australia
    J Steroid Biochem Mol Biol 101:275-85. 2006
    ..Stimulation with E2 resulted in the synthesis of additional isoforms of increased acidity, which may represent a type of CBG only seen during pregnancy in vivo. Possible physiological implications of these findings are discussed...
  29. ncbi request reprint Effects of St John's wort (Hypericum perforatum L.) extract on plasma androgen concentrations in healthy men and women: a pilot study
    Jennifer L Donovan
    Laboratory of Drug Disposition and Pharmacogenetics, Department of Psychiatry and Behavioral Sciences, Medical University of South Carolina, North Charleston, SC 29425, USA
    Phytother Res 19:901-6. 2005
    ....
  30. ncbi request reprint Effect of Ginkgo biloba extract on plasma steroid concentrations in healthy volunteers: a pilot study
    John S Markowitz
    Department of Pharmaceutical Sciences, Medical University of South Carolina, Children s Research Institute, Charleston, South Carolina 29425, USA
    Pharmacotherapy 25:1337-40. 2005
    ..To determine if a standardized ginkgo supplement significantly alters concentrations of circulating androgenic steroids in humans...
  31. ncbi request reprint Measuring cortisol in human psychobiological studies
    Ari Levine
    Interdisciplinary Program in the Brain Sciences, Bar Ilan University, Ramat Gan, 52900, Israel
    Physiol Behav 90:43-53. 2007
    ..These points are raised with special emphasis on their significance during pregnancy and the post-partum. Such information may prove useful in informing researcher's cortisol-related protocols and in the interpretation of cortisol data...
  32. ncbi request reprint Human sperm sex hormone-binding globulin isoform: characterization and measurement by time-resolved fluorescence immunoassay
    David M Selva
    Department of Obstetrics, Child and Family Research Institute, 950 West 28th Avenue, Vancouver, British Columbia, Canada V5Z 4H4
    J Clin Endocrinol Metab 90:6275-82. 2005
    ..SHBG gene expression in human testis results in an SHBG isoform that accumulates in the sperm head...
  33. ncbi request reprint Rheumatoid factor and false positive sex-hormone binding globulin
    John G Lewis
    Clin Chim Acta 332:139-41; author reply 143-4. 2003