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Genomes and GenesSpecies | John G LewisSummaryCountry: New Zealand Publications
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An enzyme-linked immunosorbent assay for corticosteroid-binding globulin using monoclonal and polyclonal antibodies: decline in CBG following synthetic ACTHJohn G Lewis
Steroid and Immunobiochemistry Laboratory, Canterbury Health Laboratories, PO Box 151, Christchurch, New Zealand
Clin Chim Acta 328:121-8. 2003..We have developed and validated an enzyme-linked immunosorbent assay (ELISA) for CBG and together with plasma cortisol calculated the "free cortisol index" as an additional parameter for assessing adrenal function...- Plasma variation of corticosteroid-binding globulin and sex hormone-binding globulinJ G Lewis
Steroid and Immunobiochemistry Laboratory, Canterbury Health Laboratories, Christchurch, New Zealand
Horm Metab Res 38:241-5. 2006..The consequence of lower levels of CBG in these obese male subjects is reflected by higher levels of circulating free cortisol, potentially offering a more favourable environment for adipogenesis... 
Plasma retinol-binding protein is unlikely to be a useful marker of insulin resistanceJohn G Lewis
Steroid and Immunobiochemistry Laboratory, Canterbury Health Laboratories, Christchurch 8001, New Zealand
Diabetes Res Clin Pract 80:e13-5. 2008..We confirm, using the largest study cohort to date, that plasma RBP is unlikely to be a useful marker of insulin resistance...- Corticosteroid-binding globulin reactive centre loop antibodies recognise only the intact natured protein: elastase cleaved and uncleaved CBG may coexist in circulationJohn G Lewis
Steroid and Immunobiochemistry Laboratory, Canterbury Health Laboratories, PO Box 151, Christchurch, New Zealand
J Steroid Biochem Mol Biol 127:289-94. 2011..This antibody could be used for determining the time course of intact CBG in various relevant patient cohorts and for structure/function studies on the biology of human CBG... - Intact or "active" corticosteroid-binding globulin (CBG) and total CBG in plasma: determination by parallel ELISAs using monoclonal antibodiesJohn G Lewis
Steroid and Immunobiochemistry Laboratory, Canterbury Health Laboratories, Christchurch, New Zealand
Clin Chim Acta 416:26-30. 2013..Gross differences in total and intact CBG levels may not be due to differences in N-glycosylation affecting antibody binding as CBG levels are unaffected by PNGase F treatment... - Plasma retinol-binding protein is not a marker of insulin resistance in overweight subjects: a three year longitudinal studyJohn G Lewis
Steroid and Immunobiochemistry Laboratory, Canterbury Health Laboratories, P O Box 151, Christchurch, New Zealand
Clin Biochem 41:1034-8. 2008..This longitudinal study investigated whether or not plasma retinol-binding protein (RBP), recently referred to as RBP4, was a marker of insulin resistance in overweight subjects... - Plasma levels of sex hormone-binding globulin, corticosteroid-binding globulin and cortisol in overweight subjects who develop impaired fasting glucose: a 3-year prospective studyJ G Lewis
Steroid and Immunobiochemistry Laboratory, Canterbury Health Laboratories, Christchurch, New Zealand
Horm Metab Res 41:255-9. 2009.... - ZZ polyester beads: an efficient and simple method for purifying IgG from mouse hybridoma supernatantsJohn G Lewis
Steroid and Immunobiochemistry Laboratory, Canterbury Health Laboratories, Christchurch, New Zealand
J Immunol Methods 346:71-4. 2009..As a negative control we used culture supernatant from an IgM secreting hybridoma and showed that it did not bind to ZZ polyester beads... - Circulating levels of isoflavones and markers of 5alpha-reductase activity are higher in Japanese compared with New Zealand males: what is the role of circulating steroids in prostate disease?J G Lewis
Steroid and Immunobiochemistry Laboratory, Canterbury Health Laboratories, P O Box 151, Christchurch 8001, New Zealand
Steroids 70:974-9. 2005..Significantly the positive association between isoflavones levels and 5alpha-steroids is counter-intuitive to isoflavone intake offering prostate protection, unless this is postulated to occur through other mechanisms... - An ELISA for plasma retinol-binding protein using monoclonal and polyclonal antibodies: plasma variation in normal and insulin resistant subjectsJohn G Lewis
Steroid and Immunobiochemistry Laboratory, Canterbury Health Laboratories, Christchurch, New Zealand
Clin Biochem 40:828-34. 2007..We examined this in normal subjects and compared plasma levels and variability in lean subjects and subjects with the metabolic syndrome... - Plasma free cortisol fraction reflects levels of functioning corticosteroid-binding globulinJohn G Lewis
Steroid and Immunobiochemistry Laboratory, Canterbury Health Laboratories, PO Box 151 Christchurch, New Zealand
Clin Chim Acta 359:189-94. 2005..However little is known about the distribution of free cortisol in plasma where CBG is inactivated or in congenital CBG deficiency... - Plasma sex hormone-binding globulin rather than corticosteroid-binding globulin is a marker of insulin resistance in obese adult malesJ G Lewis
Steroid and Immunobiochemistry Laboratory, Canterbury Health Laboratories, Christchurch, New Zealand
Diabetes Obes Metab 6:259-63. 2004..The aim of this study was to test the hypothesis that these steroid-binding proteins are markers of insulin resistance and obesity in adult patients with the metabolic syndrome... - Corticosteroid-binding globulin, cortisol, free cortisol, and sex hormone-binding globulin responses following oral glucose challenge in spinal cord-injured and able-bodied menJ G Lewis
Steroid and Immunobiochemistry Laboratory, Canterbury Health Laboratories, Christchurch, New Zealand
Horm Metab Res 42:882-6. 2010..There were no significant changes in plasma sex hormone-binding globulin in either group... - Caution on the use of saliva measurements to monitor absorption of progesterone from transdermal creams in postmenopausal womenJohn G Lewis
Steroid and Immunobiochemistry Laboratory, Canterbury Health Laboratories, P O Box 151, Christchurch, New Zealand
Maturitas 41:1-6. 2002..To determine the levels of progesterone in plasma, red cells and saliva as well as pregnanediol-3-glucuronide excretion in postmenopausal women using transdermal progesterone creams... - Is late-night salivary cortisol a better screening test for possible cortisol excess than standard screening tests in obese patients with Type 2 diabetes?Elna Ellis
Christchurch Hospital, Christchurch, New Zealand
N Z Med J 125:47-58. 2012.... - An enzyme-linked immunosorbent assay (ELISA) for methylphenidate (Ritalin ) in urineMark G Lewis
Canterbury Health Laboratories, Christchurch, New Zealand
J Anal Toxicol 27:342-5. 2003..This method is unaffected by drugs of abuse and is suitable for routine use in the toxicology laboratory... - A case of low cortisol-binding globulin: use of plasma free cortisol in interpretation of hypothalamic-pituitary-adrenal axis testsJames S Davidson
Department of Chemical Pathology, LabPlus, Auckland City Hospital, Auckland, New Zealand
Ann Clin Biochem 43:237-9. 2006..Measurement of CBG concentration and calculation of free cortisol or free cortisol index can help avoid false interpretations of dynamic tests of the HPA axis based on plasma total cortisol... - Comparison of indices of insulin resistance with metabolic syndrome classifications to predict the development of impaired fasting glucose in overweight and obese subjects: a 3-year prospective studyB I Shand
Lipid and Diabetes Research Group, Christchurch Hospital, Christchurch, New Zealand
Int J Obes (Lond) 33:1274-9. 2009..To compare the ability of biochemical indices of insulin resistance (IR) with metabolic syndrome (MetS) classifications to predict changes in blood glucose control over a 3-year period in overweight and obese subjects... - Monoclonal antibodies to human sex hormone-binding globulin (SHBG): characterization and use in a simple enzyme-linked immunosorbent assay (ELISA) of SHBG in plasmaJ G Lewis
Steroid and Immunobiochemistry Laboratory, Canterbury Health Laboratories, Christchurch, New Zealand
Steroids 64:259-65. 1999..The monoclonal antibody-based ELISA shows excellent performance characteristics and is unaffected by added testosterone or estradiol... - Two cases of adrenal suppression following a Chinese herbal remedy: a cause for concern?C M Florkowski
Canterbury Health Laboratories, Christchurch Hospital
N Z Med J 115:223-4. 2002 - Immune reactivity of four monoclonal and two polyclonal antibodies raised against recombinant pneumococcal surface adhesin AAnja M Werno
Microbiology Unit, Canterbury Health Laboratories, Christchurch, New Zealand
Hybridoma (Larchmt) 31:168-75. 2012..The specificity of the four MAbs appears to be high as they only recognized S. pneumoniae and S. pseudopneumoniae. However, further testing of closely related streptococcal species is necessary to confirm this finding... - Plasma sex hormone-binding globulin, corticosteroid-binding globulin, cortisol, and free cortisol levels in outpatients attending a lipid disorders clinic: a cross-sectional study of 1137 subjectsJ G Lewis
Department of Clinical Biochemistry, Canterbury Health Laboratories, Christchurch, New Zealand
Horm Metab Res 42:274-9. 2010..However, the aetiological role of CBG and cortisol in insulin resistance is uncertain, although in males, cortisol and CBG could be subtly related to the degree of insulin resistance... - Plasma betaine concentrations correlate with plasma cortisol but not with C-reactive protein in an elderly populationMichael Lever
Clinical Biochemistry Unit, Canterbury Health Laboratories, Christchurch, New Zealand
Clin Chem Lab Med 50:1635-40. 2012..We aimed to compare plasma cortisol (a stress marker) and C-reactive protein (an inflammation marker) as statistical predictors of plasma betaine concentrations... - A patient with pseudo-Addison's disease and falsely elevated thyroxine due to interference in serum cortisol and free thyroxine immunoassays by two different mechanismsMohamed Saleem
Canterbury Health Laboratories, Christchurch, New Zealand
Ann Clin Biochem 46:172-5. 2009..Good clinician-laboratory interface and laboratory work-up of patients with results that are discordant from the clinical findings can reduce unnecessary investigation and inappropriate treatment... - The effect of isoflavone extract ingestion, as Trinovin, on plasma steroids in normal menJohn G Lewis
Steroid and Immunobiochemistry Laboratory, Canterbury Health Laboratories, Christchurch, New Zealand
Steroids 67:25-9. 2002..In contrast the increase in dihydrotestosterone plasma levels could be seen as possibly detrimental... - Reduced maternal corticosteroid-binding globulin and cortisol levels in pre-eclampsia and gamete recipient pregnanciesJui T Ho
Hanson Institute, University of Adelaide, and Endocrine and Metabolic Unit of the Royal Adelaide Hospital, Adelaide, South Australia, Australia
Clin Endocrinol (Oxf) 66:869-77. 2007..To measure and contrast maternal cortisol and corticosteroid-binding globulin (CBG) levels in pregnancies with normal outcomes, pre-eclampsia, intrauterine growth restriction (IUGR) and in gamete recipients... - Effect of Ginkgo biloba extract on plasma steroid concentrations in healthy volunteers: a pilot studyJohn S Markowitz
Department of Pharmaceutical Sciences, Medical University of South Carolina, Children s Research Institute, Charleston, South Carolina 29425, USA
Pharmacotherapy 25:1337-40. 2005..To determine if a standardized ginkgo supplement significantly alters concentrations of circulating androgenic steroids in humans... - Effects of St John's wort (Hypericum perforatum L.) extract on plasma androgen concentrations in healthy men and women: a pilot studyJennifer L Donovan
Laboratory of Drug Disposition and Pharmacogenetics, Department of Psychiatry and Behavioral Sciences, Medical University of South Carolina, North Charleston, SC 29425, USA
Phytother Res 19:901-6. 2005.... - Hormonal effects on the secretion and glycoform profile of corticosteroid-binding globulinRobin Mihrshahi
Department of Biological Sciences, Macquarie University, Sydney, NSW 2109, Australia
J Steroid Biochem Mol Biol 101:275-85. 2006..Stimulation with E2 resulted in the synthesis of additional isoforms of increased acidity, which may represent a type of CBG only seen during pregnancy in vivo. Possible physiological implications of these findings are discussed... - Measuring cortisol in human psychobiological studiesAri Levine
Interdisciplinary Program in the Brain Sciences, Bar Ilan University, Ramat Gan, 52900, Israel
Physiol Behav 90:43-53. 2007..These points are raised with special emphasis on their significance during pregnancy and the post-partum. Such information may prove useful in informing researcher's cortisol-related protocols and in the interpretation of cortisol data... - Rheumatoid factor and false positive sex-hormone binding globulinJohn G Lewis
Clin Chim Acta 332:139-41; author reply 143-4. 2003 - Human sperm sex hormone-binding globulin isoform: characterization and measurement by time-resolved fluorescence immunoassayDavid M Selva
Department of Obstetrics, Child and Family Research Institute, 950 West 28th Avenue, Vancouver, British Columbia, Canada V5Z 4H4
J Clin Endocrinol Metab 90:6275-82. 2005..Sperm SHBG isoform levels correlate significantly with age and sperm motility and may influence sperm function in relation to male fertility...