Fully human monoclonal antibody directed to proteolytic cleavage site in severe acute respiratory syndrome (SARS) coronavirus S protein neutralizes the virus in a rhesus macaque SARS modelTohru Miyoshi-Akiyama
Department of Infectious Diseases, National Center for Global Health and Medicine, 1 21 1 Toyama, Shinjuku ku, Tokyo 162 8655, Japan
J Infect Dis 203:1574-81. 2011
..In the present study, we evaluated the efficacy of a fully human monoclonal antibody capable of neutralizing SARS-CoV in vitro in a Rhesus macaque model of SARS...
Evolutionary paths of streptococcal and staphylococcal superantigensKayo Okumura
Department of Infectious Diseases, National Center for Global Health and Medicine, 1 21 1, Shinjuku ku, Tokyo, 162 8655, Japan
BMC Genomics 13:404. 2012
..abstract:..
- Discrimination of influenza A subtype by antibodies recognizing host-specific amino acids in the viral nucleoprotein
Tohru Miyoshi-Akiyama
Department of Infectious Diseases, National Center for Global Health and Medicine, Tokyo, Japan
Influenza Other Respi Viruses 6:434-41. 2012
....
- Complete genome sequence of Helicobacter cinaedi type strain ATCC BAA-847
Tohru Miyoshi-Akiyama
Department of Infectious Diseases, National Center for Global Health and Medicine, Toyama, Tokyo, Japan
J Bacteriol 194:5692. 2012
..The genome sequence will provide new insights into the diagnosis, pathogenic mechanisms, and drug resistance of H. cinaedi...
- Complete genome sequence of Streptococcus pyogenes M1 476, isolated from a patient with streptococcal toxic shock syndrome
Tohru Miyoshi-Akiyama
Department of Infectious Diseases, National Center for Global Health and Medicine, Shinjuku ku, Tokyo, Japan
J Bacteriol 194:5466. 2012
..The genome sequence will provide new insights into the mechanisms underlying STSS...
- Development and evaluation of a line probe assay for rapid typing of influenza viruses and detection of the H274Y mutation
Tohru Miyoshi-Akiyama
Department of Infectious Diseases, Research Institute, National Center for Global Health and Medicine, Shinjuku, Tokyo 162 8655, Japan
J Virol Methods 185:276-80. 2012
..These findings indicate that LiPA for influenza viruses may be used to monitor viral trends during the influenza season...
- Complete annotated genome sequence of Mycobacterium tuberculosis Erdman
Tohru Miyoshi-Akiyama
Department of Infectious Diseases, Research Institute, National Center for Global Health and Medicine, Shinjuku, Tokyo, Japan
J Bacteriol 194:2770. 2012
..We report the completely annotated genome sequence of Mycobacterium tuberculosis Erdman (TMC 107; ATCC 35801), which is a well-known laboratory strain of M. tuberculosis...
- Complete genome sequence of highly multidrug-resistant Pseudomonas aeruginosa NCGM2.S1, a representative strain of a cluster endemic to Japan
Tohru Miyoshi-Akiyama
Department of Infectious Diseases, Research Institute, National Center for Global Health and Medicine, 1 21 1 Toyama, Shinjuku ku, Tokyo 162 8655, Japan
J Bacteriol 193:7010. 2011
..S1, a representative strain of a cluster endemic to Japan with a high level of resistance to carbapenem (MIC ≥ 128 μg/ml), amikacin (MIC ≥ 128 μg/ml), and fluoroquinolone (MIC ≥ 128 μg/ml)...
- Genome sequence of clinical isolate Mycobacterium tuberculosis NCGM2209
Tohru Miyoshi-Akiyama
Department of Infectious Diseases, Research Institute, National Center for Global Health and Medicine, 1 21 1 Toyama, Shinjuku ku, Tokyo 162 8655, Japan
J Bacteriol 193:6792. 2011
..We report the annotated genome sequence of a clinical isolate, Mycobacterium tuberculosis strain NCGM2209, which belongs to the "Beijing family" and was isolated in Japan...
- Multidrug-resistant Pseudomonas aeruginosa strain that caused an outbreak in a neurosurgery ward and its aac(6')-Iae gene cassette encoding a novel aminoglycoside acetyltransferase
Jun ichiro Sekiguchi
Department of Infectious Diseases, Research Institute, International Medical Center of Japan, 1 21 1 Toyama, Shinjuku, Tokyo 162 8655, Japan
Antimicrob Agents Chemother 49:3734-42. 2005
..These results indicate that P. aeruginosa IMCJ2.S1 has developed multidrug resistance by acquiring resistance determinants, including a novel member of the aac(6')-I family and mutations in drug resistance genes...
- Development of an immunochromatographic assay for the rapid detection of AAC(6')-Iae-producing multidrug-resistant Pseudomonas aeruginosa
Tomoe Kitao
Department of Infectious Diseases, National Center for Global Health and Medicine, Shinjuku, Tokyo 162 8655, Japan
J Antimicrob Chemother 65:1382-6. 2010
..Here, a new immunochromatographic assay specific for aminoglycoside 6'-N-acetyltransferase AAC(6')-Iae was designed. AAC(6')-Iae is a significant marker molecule for multidrug-resistant (MDR) Pseudomonas aeruginosa isolates in Japan...
Streptococcus dysgalactiae-derived mitogen (SDM), a novel bacterial superantigen: characterization of its biological activity and predicted tertiary structureTohru Miyoshi-Akiyama
Department of Microbiology and Immunology, Tokyo Women s Medical University, 8 1 Kawada cho, Shinjuku ku, Tokyo 162 8666, Japan
Mol Microbiol 47:1589-99. 2003
..SDM showed overall structural similarity to both these superantigens. This is the first study to characterize fully a bacterial superantigen from S. dysgalactiae...
- Quantitative and qualitative comparison of virulence traits, including murine lethality, among different M types of group A streptococci
Tohru Miyoshi-Akiyama
Department of Microbiology and Immunology, Tokyo Women s Medical University, Tokyo, Japan
J Infect Dis 187:1876-87. 2003
..These results indicate that M types may serve, in part, as markers for strains/clones with particular profiles of virulence traits and mouse lethality...
- Emergence of a novel multidrug-resistant Pseudomonas aeruginosa strain producing IMP-type metallo-β-lactamases and AAC(6')-Iae in Japan
Tomoe Kitao
Department of Infectious Diseases, National Center for Global Health and Medicine, 1 21 1 Toyama, Shinjuku, Tokyo 162 8655, Japan
Int J Antimicrob Agents 39:518-21. 2012
..aeruginosa strain is emerging in Japan. Isolates belonging to both clusters were isolated from different parts of the country...
- Development and evaluation of a line probe assay for rapid identification of pncA mutations in pyrazinamide-resistant mycobacterium tuberculosis strains
Jun ichiro Sekiguchi
Department of Infectious Diseases, Research Institute, International Medical Center of Japan, 1 21 1 Toyama, Shinjuku, Tokyo 162 8655, Japan
J Clin Microbiol 45:2802-7. 2007
..This assay can be used with sputa from tuberculosis patients. It appears to be reliable and widely applicable and, given its simplicity and rapid performance, will be a valuable tool for diagnostic use...
- Monitoring of S protein maturation in the endoplasmic reticulum by calnexin is important for the infectivity of severe acute respiratory syndrome coronavirus
Masaya Fukushi
Department of Infectious Diseases, Research Institute, National Center for Global Health and Medicine, Tokyo, Japan
J Virol 86:11745-53. 2012
..In SARS-CoV, viral production was obviously inhibited. These findings demonstrated that calnexin strictly monitors the maturation of S protein by its direct binding, resulting in conferring infectivity on SARS-CoV...
- Development of an immunochromatographic assay for rapid detection of AAC(6')-Ib-producing Pseudomonas aeruginosa
Tatsuya Tada
Department of Infectious Diseases, Research Institute, National Center for Global Health and Medicine, Shinjuku, Tokyo 162 8655, Japan
J Microbiol Methods 91:114-6. 2012
..The results of the assessment were fully consistent with those of aac(6')-Ib PCR analyses...
- Cloning, expression, and characterization of the superantigen streptococcal pyrogenic exotoxin G from Streptococcus dysgalactiae
Jizi Zhao
Department of Infectious Diseases, Research Institute, International Medical Center of Japan, 1 21 1 Toyama, Shinjuku ku, Tokyo 162 8655, Japan
Infect Immun 75:1721-9. 2007
..Bovine serum showed reactivity to the rSPEGG proteins. These results indicated that SPEGGs have properties as superantigens, and it is likely that SPEGGs play a pathogenic role in animals...
- KHM-1, a novel plasmid-mediated metallo-beta-lactamase from a Citrobacter freundii clinical isolate
Jun ichiro Sekiguchi
Department of Infectious Diseases, Research Institute, International Medical Center of Japan, Shinjuku, Tokyo, Japan
Antimicrob Agents Chemother 52:4194-7. 2008
..Recombinant KHM-1 exhibited EDTA-inhibitable hydrolytic activity against most beta-lactams, with an overall preference for cephalosporins...
- Outbreaks of multidrug-resistant Pseudomonas aeruginosa in community hospitals in Japan
Jun ichiro Sekiguchi
Department of Infectious Diseases, Research Institute, International Medical Center of Japan, 1 21 1 Toyama, Shinjuku, Tokyo 162 8655, Japan
J Clin Microbiol 45:979-89. 2007
..aeruginosa occurred in community hospitals in this area. The methods developed in this study to detect aac(6')-Iae were rapid and effective in diagnosing infections caused by various MDR P. aeruginosa clones...
- Use of DNA arrays to identify a mutation in the negative regulator, csrR, responsible for the high virulence of a naturally occurring type M3 group A streptococcus clinical isolate
Tohru Miyoshi-Akiyama
Department of Infectious Diseases, International Medical Center of Japan, Tokyo, Japan
J Infect Dis 193:1677-84. 2006
..Although wt CsrR bound to the hasA promoter region, type M3-f CsrR did not. Thus, the high virulence of the type M3-f strain is associated with the decreased binding of type M3-f CsrR to its target sequences...
- Complete genome sequencing and analysis of a Lancefield group G Streptococcus dysgalactiae subsp. equisimilis strain causing streptococcal toxic shock syndrome (STSS)
Yumi Shimomura
Department of Infectious Diseases, National Center for Global Health and Medicine, 1 21 1 Toyama, Shinjuku ku, Tokyo 162 8655, Japan
BMC Genomics 12:17. 2011
..We sequenced the entire genome of SDSE strain GGS_124 isolated from a patient with STSS...
- Positive correlation between low adhesion of group A Streptococcus to mammalian cells and virulence in a mouse model
Tohru Miyoshi-Akiyama
Department of Infectious Diseases, International Medical Center of Japan, Research Institute, Toyama, Shinjuku ku, Tokyo, Japan
FEMS Microbiol Lett 293:107-14. 2009
..Taken together, these results indicate that the invasiveness of GAS, reflected as higher virulence, is correlated directly with lower adhesion to host cells...
- Laninamivir octanoate and artificial surfactant combination therapy significantly increases survival of mice infected with lethal influenza H1N1 Virus
Masaya Fukushi
Disease Control and Prevention Center, National Center for Global Health and Medicine, Tokyo, Japan
PLoS ONE 7:e42419. 2012
..Therefore, the present study examined whether combination therapy of NAI with exogenous artificial surfactant affects mortality of influenza virus-infected mice...
- Downregulation of katG expression is associated with isoniazid resistance in Mycobacterium tuberculosis
Hiroki Ando
National Center for Global Health and Medicine, 1 21 1 Toyama, Shinjuku, Tokyo 162 8655, Japan
Mol Microbiol 79:1615-28. 2011
..These results suggested that downregulation of katG is a mechanism of INH resistance in M. tuberculosis and that mutations in the furA-katG intergenic region play a role in this resistance mechanism...
- Development of an immunochromatographic assay for diagnosing the production of IMP-type metallo-β-lactamases that mediate carbapenem resistance in Pseudomonas
Tomoe Kitao
Department of Infectious Diseases, Research Institute, National Center for Global Health and Medicine, Shinjuku, Tokyo, Japan
J Microbiol Methods 87:330-7. 2011
..The detection limit of the assay was 5.7×10(4)cfu per test. Taken together, these data suggest that the developed assay can be used for rapid and reliable diagnosis of the production of IMP-type MBLs in Gram-negative bacteria...
- Development of an immunochromatographic assay specifically detecting pandemic H1N1 (2009) influenza virus
Tohru Miyoshi-Akiyama
Department of Infectious Diseases, Research Institute, International Medical Center of Japan, 1 21 1 Toyama, Shinjuku ku, Tokyo 162 8655, Japan
J Clin Microbiol 48:703-8. 2010
..This RDK, together with a new vaccine and the stockpiling of anti-influenza drugs, will make aggressive measures to contain AH1pdm infections possible...
- Genome sequence of multidrug-resistant Pseudomonas aeruginosa NCGM1179
Tatsuya Tada
Department of Infectious Diseases, Research Institute, National Center for Global Health and Medicine, 1 21 1 Toyama, Shinjuku ku, Tokyo 162 8655, Japan
J Bacteriol 193:6397. 2011
..We report the annotated genome sequence of multidrug-resistant Pseudomonas aeruginosa strain NCGM1179, which is highly resistant to carbapenems, aminoglycosides, and fluoroquinolones and is emerging at medical facilities in Japan...
- Cytocidal effect of Streptococcus pyogenes on mouse neutrophils in vivo and the critical role of streptolysin S
Tohru Miyoshi-Akiyama
Department of Infectious Diseases, Research Institute, International Medical Center of Japan, Tokyo, Japan
J Infect Dis 192:107-16. 2005
..Our findings suggest that, in addition to the production of antiphagocytic factors and survival inside phagocytes, GAS uses a more aggressive method--the elimination of neutrophils--to evade the host's innate immune system...
- Dissection and identification of regions required to form pseudoparticles by the interaction between the nucleocapsid (N) and membrane (M) proteins of SARS coronavirus
Seisuke Hatakeyama
Department of Infectious Diseases, Research Institute, International Medical Center of Japan, Tokyo, Japan
Virology 380:99-108. 2008
..These results indicated that the minimum portion of N required for the interaction with M and pseudoparticle formation consists of amino acids 301-422...
- AAC(6')-Iaf, a novel aminoglycoside 6'-N-acetyltransferase from multidrug-resistant Pseudomonas aeruginosa clinical isolates
Tomoe Kitao
Department of Infectious Diseases, Research Institute, International Medical Center of Japan, 1 21 1 Toyama, Shinjuku, Tokyo 162 8655, Japan
Antimicrob Agents Chemother 53:2327-34. 2009
..Collectively, these findings indicate that AAC(6')-Iaf contributes to aminoglycoside resistance...
- A co-stimulatory molecule on activated T cells, H4/ICOS, delivers specific signals in T(h) cells and regulates their responses
Yutaka Arimura
Departments of Microbiology and Immunology, Tokyo Women s Medical University, 8 1 Kawada cho, Shinjuku ku, Tokyo 162 8666, Japan
Int Immunol 14:555-66. 2002
..Thus, the difference in H4/ICOS expression on activated CD4(+) T cells, which is regulated among the mouse strains, may also regulate the polarization of T(h) cells...
- Serial histopathological examination of the lungs of mice infected with influenza A virus PR8 strain
Masaya Fukushi
Disease Control and Prevention Center, National Center for Global Health and Medicine, Tokyo, Japan
PLoS ONE 6:e21207. 2011
..Inhibition of the development of DAD with alveolar collapse may decrease the mortality rate in severe viral pneumonia caused by influenza virus infection...
- novel 6'-n-aminoglycoside acetyltransferase AAC(6')-Iaj from a clinical isolate of Pseudomonas aeruginosa
Tatsuya Tada
Department of Infectious Diseases, Research Institute, National Center for Global Health and Medicine, Shinjuku, Tokyo, Japan
Antimicrob Agents Chemother 57:96-100. 2013
..aeruginosa NCGM1588, including arbekacin...
- NDM-8 Metallo-β-Lactamase in a Multidrug-Resistant Escherichia coli Strain Isolated in Nepal
Tatsuya Tada
Department of Infectious Diseases, Research Institute
Antimicrob Agents Chemother 57:2394-6. 2013
..The amino acid sequence of NDM-8 has substitutions at positions 130 (Asp to Gly) and 154 (Met to Leu) compared with NDM-1. NDM-8 showed enzymatic activities against β-lactams similar to those of NDM-1...
- Detection of multidrug resistance in Mycobacterium tuberculosis
Jun ichiro Sekiguchi
International Medical Center of Japan, 1 21 1 Toyama, Shinjuku, Tokyo 162 8655, Japan
J Clin Microbiol 45:179-92. 2007
..In conclusion, our new method is useful for rapid detection of multiple-drug-resistant M. tuberculosis and for identifying novel mutations in drug-resistant M. tuberculosis...
- Cloning and characterization of a novel trimethoprim-resistant dihydrofolate reductase from a nosocomial isolate of Staphylococcus aureus CM.S2 (IMCJ1454)
Jun-ichiro Sekiguchi
Department of Infectious Diseases, Research Institute, International Medical Center of Japan, 1-21-1 Toyama, Shinjuku, Tokyo 162-8655, Japan
Antimicrob Agents Chemother 49:3948-51. 2005
..Escherichia coli expressing dfrG was highly resistant to TMP. Recombinant S3DHFR exhibited DHFR activity that was not inhibited by TMP...
- Complete genome sequence of Melissococcus plutonius ATCC 35311
Kayo Okumura
Department of Animal and Food Hygiene, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, Japan
J Bacteriol 193:4029-30. 2011
..M. plutonius is a one-genus, one-species bacterium and the etiological agent of European foulbrood of the honeybee. The genome sequence will provide new insights into the molecular mechanisms underlying its pathogenicity...
