Tohru Miyoshi-Akiyama

Summary

Publications

  1. doi request reprint Fully human monoclonal antibody directed to proteolytic cleavage site in severe acute respiratory syndrome (SARS) coronavirus S protein neutralizes the virus in a rhesus macaque SARS model
    Tohru Miyoshi-Akiyama
    Department of Infectious Diseases, National Center for Global Health and Medicine, 1 21 1 Toyama, Shinjuku ku, Tokyo 162 8655, Japan
    J Infect Dis 203:1574-81. 2011
  2. pmc Multilocus sequence typing (MLST) for characterization of Enterobacter cloacae
    Tohru Miyoshi-Akiyama
    Department of Infectious Diseases, National Center for Global Health and Medicine, Shinjuku ku, Tokyo, Japan
    PLoS ONE 8:e66358. 2013
  3. doi request reprint Discrimination of influenza A subtype by antibodies recognizing host-specific amino acids in the viral nucleoprotein
    Tohru Miyoshi-Akiyama
    Department of Infectious Diseases, National Center for Global Health and Medicine, Tokyo, Japan
    Influenza Other Respir Viruses 6:434-41. 2012
  4. pmc Complete genome sequence of Helicobacter cinaedi type strain ATCC BAA-847
    Tohru Miyoshi-Akiyama
    Department of Infectious Diseases, National Center for Global Health and Medicine, Toyama, Tokyo, Japan
    J Bacteriol 194:5692. 2012
  5. pmc Complete genome sequence of Streptococcus pyogenes M1 476, isolated from a patient with streptococcal toxic shock syndrome
    Tohru Miyoshi-Akiyama
    Department of Infectious Diseases, National Center for Global Health and Medicine, Shinjuku ku, Tokyo, Japan
    J Bacteriol 194:5466. 2012
  6. doi request reprint Development and evaluation of a line probe assay for rapid typing of influenza viruses and detection of the H274Y mutation
    Tohru Miyoshi-Akiyama
    Department of Infectious Diseases, Research Institute, National Center for Global Health and Medicine, Shinjuku, Tokyo 162 8655, Japan
    J Virol Methods 185:276-80. 2012
  7. pmc Complete annotated genome sequence of Mycobacterium tuberculosis Erdman
    Tohru Miyoshi-Akiyama
    Department of Infectious Diseases, Research Institute, National Center for Global Health and Medicine, Shinjuku, Tokyo, Japan
    J Bacteriol 194:2770. 2012
  8. pmc Genome sequence of clinical isolate Mycobacterium tuberculosis NCGM2209
    Tohru Miyoshi-Akiyama
    Department of Infectious Diseases, Research Institute, National Center for Global Health and Medicine, 1 21 1 Toyama, Shinjuku ku, Tokyo 162 8655, Japan
    J Bacteriol 193:6792. 2011
  9. pmc Complete genome sequence of highly multidrug-resistant Pseudomonas aeruginosa NCGM2.S1, a representative strain of a cluster endemic to Japan
    Tohru Miyoshi-Akiyama
    Department of Infectious Diseases, Research Institute, National Center for Global Health and Medicine, 1 21 1 Toyama, Shinjuku ku, Tokyo 162 8655, Japan
    J Bacteriol 193:7010. 2011
  10. pmc Multidrug-resistant Pseudomonas aeruginosa strain that caused an outbreak in a neurosurgery ward and its aac(6')-Iae gene cassette encoding a novel aminoglycoside acetyltransferase
    Jun ichiro Sekiguchi
    Department of Infectious Diseases, Research Institute, International Medical Center of Japan, 1 21 1 Toyama, Shinjuku, Tokyo 162 8655, Japan
    Antimicrob Agents Chemother 49:3734-42. 2005

Collaborators

Detail Information

Publications46

  1. doi request reprint Fully human monoclonal antibody directed to proteolytic cleavage site in severe acute respiratory syndrome (SARS) coronavirus S protein neutralizes the virus in a rhesus macaque SARS model
    Tohru Miyoshi-Akiyama
    Department of Infectious Diseases, National Center for Global Health and Medicine, 1 21 1 Toyama, Shinjuku ku, Tokyo 162 8655, Japan
    J Infect Dis 203:1574-81. 2011
    ..In the present study, we evaluated the efficacy of a fully human monoclonal antibody capable of neutralizing SARS-CoV in vitro in a Rhesus macaque model of SARS...
  2. pmc Multilocus sequence typing (MLST) for characterization of Enterobacter cloacae
    Tohru Miyoshi-Akiyama
    Department of Infectious Diseases, National Center for Global Health and Medicine, Shinjuku ku, Tokyo, Japan
    PLoS ONE 8:e66358. 2013
    ..The MLST scheme yielded 83 sequence types (ST) including 78 novel STs found in the clinical isolates. These findings supported the robustness of the MLST scheme developed in this study...
  3. doi request reprint Discrimination of influenza A subtype by antibodies recognizing host-specific amino acids in the viral nucleoprotein
    Tohru Miyoshi-Akiyama
    Department of Infectious Diseases, National Center for Global Health and Medicine, Tokyo, Japan
    Influenza Other Respir Viruses 6:434-41. 2012
    ....
  4. pmc Complete genome sequence of Helicobacter cinaedi type strain ATCC BAA-847
    Tohru Miyoshi-Akiyama
    Department of Infectious Diseases, National Center for Global Health and Medicine, Toyama, Tokyo, Japan
    J Bacteriol 194:5692. 2012
    ..The genome sequence will provide new insights into the diagnosis, pathogenic mechanisms, and drug resistance of H. cinaedi...
  5. pmc Complete genome sequence of Streptococcus pyogenes M1 476, isolated from a patient with streptococcal toxic shock syndrome
    Tohru Miyoshi-Akiyama
    Department of Infectious Diseases, National Center for Global Health and Medicine, Shinjuku ku, Tokyo, Japan
    J Bacteriol 194:5466. 2012
    ..The genome sequence will provide new insights into the mechanisms underlying STSS...
  6. doi request reprint Development and evaluation of a line probe assay for rapid typing of influenza viruses and detection of the H274Y mutation
    Tohru Miyoshi-Akiyama
    Department of Infectious Diseases, Research Institute, National Center for Global Health and Medicine, Shinjuku, Tokyo 162 8655, Japan
    J Virol Methods 185:276-80. 2012
    ..These findings indicate that LiPA for influenza viruses may be used to monitor viral trends during the influenza season...
  7. pmc Complete annotated genome sequence of Mycobacterium tuberculosis Erdman
    Tohru Miyoshi-Akiyama
    Department of Infectious Diseases, Research Institute, National Center for Global Health and Medicine, Shinjuku, Tokyo, Japan
    J Bacteriol 194:2770. 2012
    ..We report the completely annotated genome sequence of Mycobacterium tuberculosis Erdman (TMC 107; ATCC 35801), which is a well-known laboratory strain of M. tuberculosis...
  8. pmc Genome sequence of clinical isolate Mycobacterium tuberculosis NCGM2209
    Tohru Miyoshi-Akiyama
    Department of Infectious Diseases, Research Institute, National Center for Global Health and Medicine, 1 21 1 Toyama, Shinjuku ku, Tokyo 162 8655, Japan
    J Bacteriol 193:6792. 2011
    ..We report the annotated genome sequence of a clinical isolate, Mycobacterium tuberculosis strain NCGM2209, which belongs to the "Beijing family" and was isolated in Japan...
  9. pmc Complete genome sequence of highly multidrug-resistant Pseudomonas aeruginosa NCGM2.S1, a representative strain of a cluster endemic to Japan
    Tohru Miyoshi-Akiyama
    Department of Infectious Diseases, Research Institute, National Center for Global Health and Medicine, 1 21 1 Toyama, Shinjuku ku, Tokyo 162 8655, Japan
    J Bacteriol 193:7010. 2011
    ..S1, a representative strain of a cluster endemic to Japan with a high level of resistance to carbapenem (MIC ≥ 128 μg/ml), amikacin (MIC ≥ 128 μg/ml), and fluoroquinolone (MIC ≥ 128 μg/ml)...
  10. pmc Multidrug-resistant Pseudomonas aeruginosa strain that caused an outbreak in a neurosurgery ward and its aac(6')-Iae gene cassette encoding a novel aminoglycoside acetyltransferase
    Jun ichiro Sekiguchi
    Department of Infectious Diseases, Research Institute, International Medical Center of Japan, 1 21 1 Toyama, Shinjuku, Tokyo 162 8655, Japan
    Antimicrob Agents Chemother 49:3734-42. 2005
    ..These results indicate that P. aeruginosa IMCJ2.S1 has developed multidrug resistance by acquiring resistance determinants, including a novel member of the aac(6')-I family and mutations in drug resistance genes...
  11. pmc novel 6'-n-aminoglycoside acetyltransferase AAC(6')-Iaj from a clinical isolate of Pseudomonas aeruginosa
    Tatsuya Tada
    Department of Infectious Diseases, Research Institute, National Center for Global Health and Medicine, Shinjuku, Tokyo, Japan
    Antimicrob Agents Chemother 57:96-100. 2013
    ..aeruginosa NCGM1588, including arbekacin...
  12. ncbi request reprint Development of an immunochromatographic assay for the rapid detection of AAC(6')-Iae-producing multidrug-resistant Pseudomonas aeruginosa
    Tomoe Kitao
    Department of Infectious Diseases, National Center for Global Health and Medicine, Shinjuku, Tokyo 162 8655, Japan
    J Antimicrob Chemother 65:1382-6. 2010
    ..Here, a new immunochromatographic assay specific for aminoglycoside 6'-N-acetyltransferase AAC(6')-Iae was designed. AAC(6')-Iae is a significant marker molecule for multidrug-resistant (MDR) Pseudomonas aeruginosa isolates in Japan...
  13. ncbi request reprint Streptococcus dysgalactiae-derived mitogen (SDM), a novel bacterial superantigen: characterization of its biological activity and predicted tertiary structure
    Tohru Miyoshi-Akiyama
    Department of Microbiology and Immunology, Tokyo Women s Medical University, 8 1 Kawada cho, Shinjuku ku, Tokyo 162 8666, Japan
    Mol Microbiol 47:1589-99. 2003
    ..SDM showed overall structural similarity to both these superantigens. This is the first study to characterize fully a bacterial superantigen from S. dysgalactiae...
  14. ncbi request reprint Quantitative and qualitative comparison of virulence traits, including murine lethality, among different M types of group A streptococci
    Tohru Miyoshi-Akiyama
    Department of Microbiology and Immunology, Tokyo Women s Medical University, Tokyo, Japan
    J Infect Dis 187:1876-87. 2003
    ..These results indicate that M types may serve, in part, as markers for strains/clones with particular profiles of virulence traits and mouse lethality...
  15. pmc Evolutionary paths of streptococcal and staphylococcal superantigens
    Kayo Okumura
    Department of Infectious Diseases, National Center for Global Health and Medicine, Tokyo 162 8655, Japan
    BMC Genomics 13:404. 2012
    ..Although speG is the only SAg gene of SDSE, speG was present in only 50% of clinical SDSE strains and smez in none. In this study, we analyzed the evolutionary paths of streptococcal and staphylococcal SAgs...
  16. doi request reprint Emergence of a novel multidrug-resistant Pseudomonas aeruginosa strain producing IMP-type metallo-β-lactamases and AAC(6')-Iae in Japan
    Tomoe Kitao
    Department of Infectious Diseases, National Center for Global Health and Medicine, 1 21 1 Toyama, Shinjuku, Tokyo 162 8655, Japan
    Int J Antimicrob Agents 39:518-21. 2012
    ..aeruginosa strain is emerging in Japan. Isolates belonging to both clusters were isolated from different parts of the country...
  17. pmc IMP-43 and IMP-44 metallo-β-lactamases with increased carbapenemase activities in multidrug-resistant Pseudomonas aeruginosa
    Tatsuya Tada
    Department of Infectious Diseases, Research Institute, National Center for Global Health and Medicine, Shinjuku, Tokyo, Japan
    Antimicrob Agents Chemother 57:4427-32. 2013
    ....
  18. pmc Detection of multidrug resistance in Mycobacterium tuberculosis
    Jun ichiro Sekiguchi
    International Medical Center of Japan, 1 21 1 Toyama, Shinjuku, Tokyo 162 8655, Japan
    J Clin Microbiol 45:179-92. 2007
    ..In conclusion, our new method is useful for rapid detection of multiple-drug-resistant M. tuberculosis and for identifying novel mutations in drug-resistant M. tuberculosis...
  19. doi request reprint Concomitant regulation of host tissue-destroying virulence factors and carbohydrate metabolism during invasive diseases induced by group g streptococci
    Shinya Watanabe
    Department of Infectious Diseases, Research Institute, National Center for Global Health and Medicine
    J Infect Dis 208:1482-93. 2013
    ..Recent epidemiological studies reveal that invasive SDSE infections have been increasing in Asia, Europe, and the United States. The mechanisms and key virulence factors by which SDSE causes invasive diseases are poorly understood...
  20. pmc Monitoring of S protein maturation in the endoplasmic reticulum by calnexin is important for the infectivity of severe acute respiratory syndrome coronavirus
    Masaya Fukushi
    Department of Infectious Diseases, Research Institute, National Center for Global Health and Medicine, Tokyo, Japan
    J Virol 86:11745-53. 2012
    ..In SARS-CoV, viral production was obviously inhibited. These findings demonstrated that calnexin strictly monitors the maturation of S protein by its direct binding, resulting in conferring infectivity on SARS-CoV...
  21. doi request reprint Development of an immunochromatographic assay for rapid detection of AAC(6')-Ib-producing Pseudomonas aeruginosa
    Tatsuya Tada
    Department of Infectious Diseases, Research Institute, National Center for Global Health and Medicine, Shinjuku, Tokyo 162 8655, Japan
    J Microbiol Methods 91:114-6. 2012
    ..The results of the assessment were fully consistent with those of aac(6')-Ib PCR analyses...
  22. ncbi request reprint Use of DNA arrays to identify a mutation in the negative regulator, csrR, responsible for the high virulence of a naturally occurring type M3 group A streptococcus clinical isolate
    Tohru Miyoshi-Akiyama
    Department of Infectious Diseases, International Medical Center of Japan, Tokyo, Japan
    J Infect Dis 193:1677-84. 2006
    ..Although wt CsrR bound to the hasA promoter region, type M3-f CsrR did not. Thus, the high virulence of the type M3-f strain is associated with the decreased binding of type M3-f CsrR to its target sequences...
  23. pmc KHM-1, a novel plasmid-mediated metallo-beta-lactamase from a Citrobacter freundii clinical isolate
    Jun ichiro Sekiguchi
    Department of Infectious Diseases, Research Institute, International Medical Center of Japan, Shinjuku, Tokyo, Japan
    Antimicrob Agents Chemother 52:4194-7. 2008
    ..Recombinant KHM-1 exhibited EDTA-inhibitable hydrolytic activity against most beta-lactams, with an overall preference for cephalosporins...
  24. doi request reprint Downregulation of katG expression is associated with isoniazid resistance in Mycobacterium tuberculosis
    Hiroki Ando
    National Center for Global Health and Medicine, 1 21 1 Toyama, Shinjuku, Tokyo 162 8655, Japan
    Mol Microbiol 79:1615-28. 2011
    ..These results suggested that downregulation of katG is a mechanism of INH resistance in M. tuberculosis and that mutations in the furA-katG intergenic region play a role in this resistance mechanism...
  25. pmc Outbreaks of multidrug-resistant Pseudomonas aeruginosa in community hospitals in Japan
    Jun ichiro Sekiguchi
    Department of Infectious Diseases, Research Institute, International Medical Center of Japan, 1 21 1 Toyama, Shinjuku, Tokyo 162 8655, Japan
    J Clin Microbiol 45:979-89. 2007
    ..aeruginosa occurred in community hospitals in this area. The methods developed in this study to detect aac(6')-Iae were rapid and effective in diagnosing infections caused by various MDR P. aeruginosa clones...
  26. doi request reprint Development of an immunochromatographic assay for diagnosing the production of IMP-type metallo-β-lactamases that mediate carbapenem resistance in Pseudomonas
    Tomoe Kitao
    Department of Infectious Diseases, Research Institute, National Center for Global Health and Medicine, Shinjuku, Tokyo, Japan
    J Microbiol Methods 87:330-7. 2011
    ..The detection limit of the assay was 5.7×10(4)cfu per test. Taken together, these data suggest that the developed assay can be used for rapid and reliable diagnosis of the production of IMP-type MBLs in Gram-negative bacteria...
  27. pmc NDM-1 Metallo-β-Lactamase and ArmA 16S rRNA methylase producing Providencia rettgeri clinical isolates in Nepal
    Tatsuya Tada
    Department of Infectious Diseases, Research Institute, National Center for Global Health and Medicine, 1 21 1 Toyama, Shinjuku, Tokyo 162 8655, Japan
    BMC Infect Dis 14:56. 2014
    ..We analyzed P. rettgeri clinical isolates with resistance to carbapenems and aminoglycosides in a hospital in Nepal...
  28. pmc Laninamivir octanoate and artificial surfactant combination therapy significantly increases survival of mice infected with lethal influenza H1N1 Virus
    Masaya Fukushi
    Disease Control and Prevention Center, National Center for Global Health and Medicine, Tokyo, Japan
    PLoS ONE 7:e42419. 2012
    ..Therefore, the present study examined whether combination therapy of NAI with exogenous artificial surfactant affects mortality of influenza virus-infected mice...
  29. pmc Development and evaluation of a line probe assay for rapid identification of pncA mutations in pyrazinamide-resistant mycobacterium tuberculosis strains
    Jun ichiro Sekiguchi
    Department of Infectious Diseases, Research Institute, International Medical Center of Japan, 1 21 1 Toyama, Shinjuku, Tokyo 162 8655, Japan
    J Clin Microbiol 45:2802-7. 2007
    ..This assay can be used with sputa from tuberculosis patients. It appears to be reliable and widely applicable and, given its simplicity and rapid performance, will be a valuable tool for diagnostic use...
  30. ncbi request reprint Cytocidal effect of Streptococcus pyogenes on mouse neutrophils in vivo and the critical role of streptolysin S
    Tohru Miyoshi-Akiyama
    Department of Infectious Diseases, Research Institute, International Medical Center of Japan, Tokyo, Japan
    J Infect Dis 192:107-16. 2005
    ..Our findings suggest that, in addition to the production of antiphagocytic factors and survival inside phagocytes, GAS uses a more aggressive method--the elimination of neutrophils--to evade the host's innate immune system...
  31. doi request reprint Positive correlation between low adhesion of group A Streptococcus to mammalian cells and virulence in a mouse model
    Tohru Miyoshi-Akiyama
    Department of Infectious Diseases, International Medical Center of Japan, Research Institute, Toyama, Shinjuku ku, Tokyo, Japan
    FEMS Microbiol Lett 293:107-14. 2009
    ..Taken together, these results indicate that the invasiveness of GAS, reflected as higher virulence, is correlated directly with lower adhesion to host cells...
  32. pmc NDM-8 metallo-β-lactamase in a multidrug-resistant Escherichia coli strain isolated in Nepal
    Tatsuya Tada
    Department of Infectious Diseases, Research Institute, National Center for Global Health and Medicine, Toyama, Shinjuku, Tokyo, Japan
    Antimicrob Agents Chemother 57:2394-6. 2013
    ..The amino acid sequence of NDM-8 has substitutions at positions 130 (Asp to Gly) and 154 (Met to Leu) compared with NDM-1. NDM-8 showed enzymatic activities against β-lactams similar to those of NDM-1...
  33. pmc Cloning, expression, and characterization of the superantigen streptococcal pyrogenic exotoxin G from Streptococcus dysgalactiae
    Jizi Zhao
    Department of Infectious Diseases, Research Institute, International Medical Center of Japan, 1 21 1 Toyama, Shinjuku ku, Tokyo 162 8655, Japan
    Infect Immun 75:1721-9. 2007
    ..Bovine serum showed reactivity to the rSPEGG proteins. These results indicated that SPEGGs have properties as superantigens, and it is likely that SPEGGs play a pathogenic role in animals...
  34. pmc Serial histopathological examination of the lungs of mice infected with influenza A virus PR8 strain
    Masaya Fukushi
    Disease Control and Prevention Center, National Center for Global Health and Medicine, Tokyo, Japan
    PLoS ONE 6:e21207. 2011
    ..Inhibition of the development of DAD with alveolar collapse may decrease the mortality rate in severe viral pneumonia caused by influenza virus infection...
  35. pmc Development of an immunochromatographic assay specifically detecting pandemic H1N1 (2009) influenza virus
    Tohru Miyoshi-Akiyama
    Department of Infectious Diseases, Research Institute, International Medical Center of Japan, 1 21 1 Toyama, Shinjuku ku, Tokyo 162 8655, Japan
    J Clin Microbiol 48:703-8. 2010
    ..This RDK, together with a new vaccine and the stockpiling of anti-influenza drugs, will make aggressive measures to contain AH1pdm infections possible...
  36. doi request reprint Dissection and identification of regions required to form pseudoparticles by the interaction between the nucleocapsid (N) and membrane (M) proteins of SARS coronavirus
    Seisuke Hatakeyama
    Department of Infectious Diseases, Research Institute, International Medical Center of Japan, Tokyo, Japan
    Virology 380:99-108. 2008
    ..These results indicated that the minimum portion of N required for the interaction with M and pseudoparticle formation consists of amino acids 301-422...
  37. pmc Genome sequence of multidrug-resistant Pseudomonas aeruginosa NCGM1179
    Tatsuya Tada
    Department of Infectious Diseases, Research Institute, National Center for Global Health and Medicine, 1 21 1 Toyama, Shinjuku ku, Tokyo 162 8655, Japan
    J Bacteriol 193:6397. 2011
    ..We report the annotated genome sequence of multidrug-resistant Pseudomonas aeruginosa strain NCGM1179, which is highly resistant to carbapenems, aminoglycosides, and fluoroquinolones and is emerging at medical facilities in Japan...
  38. ncbi request reprint A co-stimulatory molecule on activated T cells, H4/ICOS, delivers specific signals in T(h) cells and regulates their responses
    Yutaka Arimura
    Departments of Microbiology and Immunology, Tokyo Women s Medical University, 8 1 Kawada cho, Shinjuku ku, Tokyo 162 8666, Japan
    Int Immunol 14:555-66. 2002
    ..Thus, the difference in H4/ICOS expression on activated CD4(+) T cells, which is regulated among the mouse strains, may also regulate the polarization of T(h) cells...
  39. pmc Dissemination of 16S rRNA methylase ArmA-producing acinetobacter baumannii and emergence of OXA-72 carbapenemase coproducers in Japan
    Tatsuya Tada
    Department of Infectious Diseases, Research Institute, National Center for Global Health and Medicine, Shinjuku, Tokyo, Japan
    Antimicrob Agents Chemother 58:2916-20. 2014
    ..These findings show that A. baumannii isolates harboring armA are disseminated throughout Japan, and this is the first report to show that A. baumannii strains harboring blaOXA-72 and armA are emerging in hospitals in Japan. ..
  40. pmc Complete genome sequencing and analysis of a Lancefield group G Streptococcus dysgalactiae subsp. equisimilis strain causing streptococcal toxic shock syndrome (STSS)
    Yumi Shimomura
    Department of Infectious Diseases, National Center for Global Health and Medicine, 1 21 1 Toyama, Shinjuku ku, Tokyo 162 8655, Japan
    BMC Genomics 12:17. 2011
    ..We sequenced the entire genome of SDSE strain GGS_124 isolated from a patient with STSS...
  41. doi request reprint A silent mutation in mabA confers isoniazid resistance on Mycobacterium tuberculosis
    Hiroki Ando
    Department of Infectious Diseases, Research Institute, National Center for Global Health and Medicine, 1 21 1 Toyama, Shinjuku, Tokyo, 162 8655, Japan
    Mol Microbiol 91:538-47. 2014
    ..The mabA(g609a) silent mutation results in a novel mechanism of INH resistance, filling in a missing piece of INH resistance in Mtb. ..
  42. pmc AAC(6')-Iaf, a novel aminoglycoside 6'-N-acetyltransferase from multidrug-resistant Pseudomonas aeruginosa clinical isolates
    Tomoe Kitao
    Department of Infectious Diseases, Research Institute, International Medical Center of Japan, 1 21 1 Toyama, Shinjuku, Tokyo 162 8655, Japan
    Antimicrob Agents Chemother 53:2327-34. 2009
    ..Collectively, these findings indicate that AAC(6')-Iaf contributes to aminoglycoside resistance...
  43. ncbi request reprint Parvimonas micra as a causative organism of spondylodiscitis: a report of two cases and a literature review
    Haruka Uemura
    Disease Control and Prevention Center, National Center for Global Health and Medicine, 1 21 1 Toyama, Shinjuku ku, Tokyo, 162 8655, Japan Electronic address
    Int J Infect Dis 23:53-5. 2014
    ..This report of the detection and treatment of two cases of spondylodiscitis due to P. micra and review of the literature indicates that the use of gene sequencing methods might improve the accuracy of diagnosing this infection. ..
  44. pmc Cloning and characterization of a novel trimethoprim-resistant dihydrofolate reductase from a nosocomial isolate of Staphylococcus aureus CM.S2 (IMCJ1454)
    Jun ichiro Sekiguchi
    Department of Infectious Diseases, Research Institute, International Medical Center of Japan, 1 21 1 Toyama, Shinjuku, Tokyo 162 8655, Japan
    Antimicrob Agents Chemother 49:3948-51. 2005
    ..Escherichia coli expressing dfrG was highly resistant to TMP. Recombinant S3DHFR exhibited DHFR activity that was not inhibited by TMP...
  45. pmc Complete genome sequence of Melissococcus plutonius ATCC 35311
    Kayo Okumura
    Department of Animal and Food Hygiene, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, Japan
    J Bacteriol 193:4029-30. 2011
    ..M. plutonius is a one-genus, one-species bacterium and the etiological agent of European foulbrood of the honeybee. The genome sequence will provide new insights into the molecular mechanisms underlying its pathogenicity...