Associations between genital tract infections, genital tract inflammation, and cervical cytobrush HIV-1 DNA in US versus Kenyan womenCaroline Mitchell
Department of Obstetrics and Gynecology, University of Washington, Seattle, WA 98104, USA
J Acquir Immune Defic Syndr 62:143-8. 2013
..01). HIV-1 DNA shedding was associated with increased concentrations of IL-1β and IL-6 and lower secretory leukocyte protease inhibitor among US women but not Kenyan women...
- Effect of sexual debut on vaginal microbiota in a cohort of young women
Caroline M Mitchell
Department of Obstetrics and Gynecology and Fred Hutchinson Cancer Research Institute, University of Washington, Seattle, Washington, USA
Obstet Gynecol 120:1306-13. 2012
..Bacterial vaginosis is uncommon in women who are virgins. We estimated effects of sexual debut on vaginal bacterial colonization...
- Effect of sexual activity on vaginal colonization with hydrogen peroxide-producing lactobacilli and Gardnerella vaginalis
Caroline Mitchell
Department of Obstetrics and Gynecology, University of Washington, Seattle, WA 98195, USA
Sex Transm Dis 38:1137-44. 2011
..Bacterial vaginosis is associated with sexual activity, but mechanisms for this association are unclear...
- Cervicovaginal shedding of HIV type 1 is related to genital tract inflammation independent of changes in vaginal microbiota
Caroline Mitchell
University of Washington, Seattle, 98104, USA
AIDS Res Hum Retroviruses 27:35-9. 2011
..This association suggests that there may be additional, noninfectious causes of inflammation that increase cervicovaginal HIV-1 shedding...
- Detection of hydrogen peroxide-producing Lactobacillus species in the vagina: a comparison of culture and quantitative PCR among HIV-1 seropositive women
Jennifer E Balkus
Department of Epidemiology, University of Washington, Box 359909, 325 9th Avenue, Seattle, WA 98104, USA
BMC Infect Dis 12:188. 2012
..We assessed the concordance of H(2)O(2)-producing Lactobacillus detected by culture with quantitative PCR (qPCR) detection of Lactobacillus species commonly assumed to be H(2)O(2)-producers...
- Interaction between lactobacilli, bacterial vaginosis-associated bacteria, and HIV Type 1 RNA and DNA Genital shedding in U.S. and Kenyan women
Caroline Mitchell
Department of Obstetrics and Gynecology, University of Washington, Seattle, WA, USA
AIDS Res Hum Retroviruses 29:13-9. 2013
..6; 0.40, 0.91). Vaginal Lactobacillus species were associated with lower risk of genital HIV-1 shedding, while the presence of certain BV-associated species may increase that risk...
- Behavioral predictors of colonization with Lactobacillus crispatus or Lactobacillus jensenii after treatment for bacterial vaginosis: a cohort study
Caroline Mitchell
Harborview Women s Clinic, Department of Obstetrics and Gynecology, University of Washington, Seattle, WA 98105, USA
Infect Dis Obstet Gynecol 2012:706540. 2012
..Evaluate predictors of vaginal colonization with lactobacilli after treatment for bacterial vaginosis (BV)...
- Estimating volume of cervicovaginal secretions in cervicovaginal lavage fluid collected for measurement of genital HIV-1 RNA levels in women
Caroline Mitchell
University of Washington, Department of Obstetrics and Gynecology, Harborview Medical Center, 325 9th Ave, Box 359865, Seattle, WA 98104, USA
J Clin Microbiol 49:735-6. 2011
..Thirty-eight women provided 275 cervicovaginal specimens. The estimated median volume of cervicovaginal fluid was 0.51 ml (interquartile range, 0.33, 0.69)...
- Detection of fastidious vaginal bacteria in women with HIV infection and bacterial vaginosis
Caroline Mitchell
Department of Obstetrics and Gynecology, University of Washington, P O Box 356460, Seattle, WA 98195, USA
Infect Dis Obstet Gynecol 2009:236919. 2009
..Fastidious bacteria have been associated with bacterial vaginosis (BV) using PCR methods. We assessed the prevalence of these bacteria in HIV-1 infected women and their relationship with vaginal pH and shedding of HIV-1 RNA...
Associations between vaginal bacteria and levels of vaginal defensins in pregnant womenCaroline Mitchell
Department of Obstetrics and Gynecology, Harborview Women s Clinic, University of Washington School of Medicine, Seattle, WA, USA
Am J Obstet Gynecol 208:132.e1-7. 2013
..We evaluated vaginal defensin concentrations and levels of bacterial vaginosis-associated bacterial species in pregnant women...
- Effects of pregnancy and bacterial vaginosis on proinflammatory cytokine and secretory leukocyte protease inhibitor concentrations in vaginal secretions
Jennifer Balkus
Department of Obstetrics and Gynecology, University of Washington, Box 356460, Seattle, WA 98195, USA
J Pregnancy 2010:385981. 2010
..Pregnancy and BV were both independently associated with increased vaginal concentrations of IL-1β and IL-8; pregnant women had increased concentrations of SLPI, while women with BV had decreased SLPI concentrations...
- Changes in the vaginal microenvironment with metronidazole treatment for bacterial vaginosis in early pregnancy
Caroline Mitchell
Department of Obstetrics and Gynecology, University of Washington, Seattle, Washington 98195, USA
J Womens Health (Larchmt) 18:1817-24. 2009
..We examined whether oral or vaginal metronidazole treatment for BV in early pregnancy was more effective in restoring the normal vaginal environment...
- Cross-contamination during processing of dried blood spots used for rapid diagnosis of HIV-1 infection of infants is rare and avoidable
Caroline Mitchell
University of Washington, Department of Obstetrics and Gynecology, 1959 NE Pacific St, Seattle, WA 98195, United States
J Virol Methods 163:489-91. 2010
..This study outlines procedures that minimize false-positive results for nested PCR of HIV-1 DNA from DBS...
