Harald Mischak

Summary

Publications

  1. pmc CKD273, a new proteomics classifier assessing CKD and its prognosis
    Angel Argiles
    RD Nephrologie, Montpellier, France
    PLoS ONE 8:e62837. 2013
  2. doi request reprint Profiling of rat urinary proteomic patterns associated with drug-induced nephrotoxicity using CE coupled with MS as a potential model for detection of drug-induced adverse effects
    Harald Mischak
    Mosaiques diagnostics GmbH, Hannover, Germany
    Proteomics Clin Appl 3:1062-71. 2009
  3. doi request reprint Clinical proteomics: A need to define the field and to begin to set adequate standards
    Harald Mischak
    Mosaiques Diagnostics, Hannover, Germany
    Proteomics Clin Appl 1:148-56. 2007
  4. ncbi request reprint Proteomics in uremia and renal disease
    Harald Mischak
    Mosaiques Diagnostics and Therapeutics AG, Hannover, Germany
    Semin Dial 22:409-16. 2009
  5. pmc Capillary electrophoresis-mass spectrometry as a powerful tool in biomarker discovery and clinical diagnosis: an update of recent developments
    Harald Mischak
    Mosaiques Diagnostics and Therapeutics, Hannover, Germany
    Mass Spectrom Rev 28:703-24. 2009
  6. ncbi request reprint Advances in urinary proteome analysis and biomarker discovery
    Danilo Fliser
    Mosaiques Diagnostics and Therapeutics AG, Mellendorfer Strasse 7 9, D 30625 Hannover, Germany
    J Am Soc Nephrol 18:1057-71. 2007
  7. ncbi request reprint Proteomic patterns established with capillary electrophoresis and mass spectrometry for diagnostic purposes
    Eva M Weissinger
    Mosaiques Diagnostics and Therapeutics AG, Hannover, Germany
    Kidney Int 65:2426-34. 2004
  8. ncbi request reprint Urinary proteome analysis identifies infants but not older children requiring pyeloplasty
    Jens Drube
    Department of Pediatric Kidney, Liver and Metabolic Diseases, Children s Hospital, Hannover Medical School, Hannover, Germany
    Pediatr Nephrol 25:1673-8. 2010
  9. doi request reprint Quantitative urinary proteome analysis for biomarker evaluation in chronic kidney disease
    Justyna Jantos-Siwy
    Mosaiques Diagnostics and Therapeutics, Hannover, Germany
    J Proteome Res 8:268-81. 2009
  10. ncbi request reprint Urine protein patterns can serve as diagnostic tools in patients with IgA nephropathy
    Marion Haubitz
    Department of Nephrology, Hannover Medical School, Hannover, Germany
    Kidney Int 67:2313-20. 2005

Detail Information

Publications53

  1. pmc CKD273, a new proteomics classifier assessing CKD and its prognosis
    Angel Argiles
    RD Nephrologie, Montpellier, France
    PLoS ONE 8:e62837. 2013
    ..Recently defined in a large population, CKD273 is the first proteomic-based classifier successfully tested for prognosis of CKD progression in an independent cohort...
  2. doi request reprint Profiling of rat urinary proteomic patterns associated with drug-induced nephrotoxicity using CE coupled with MS as a potential model for detection of drug-induced adverse effects
    Harald Mischak
    Mosaiques diagnostics GmbH, Hannover, Germany
    Proteomics Clin Appl 3:1062-71. 2009
    ..This study aiming at the definition of biomarkers for drug-induced cytotoxicity may serve as a proof-of-principle for the use of urinary proteomics in assessment of drug-induced nephrotoxicity...
  3. doi request reprint Clinical proteomics: A need to define the field and to begin to set adequate standards
    Harald Mischak
    Mosaiques Diagnostics, Hannover, Germany
    Proteomics Clin Appl 1:148-56. 2007
    ..Rather, the essence of clinical proteomics should be to address clinically relevant questions and to improve the state-of-the-art, both in diagnosis and in therapy of diseases...
  4. ncbi request reprint Proteomics in uremia and renal disease
    Harald Mischak
    Mosaiques Diagnostics and Therapeutics AG, Hannover, Germany
    Semin Dial 22:409-16. 2009
    ..Finally, several examples on the application of CE-MS to determine biomarkers for disease diagnosis, prognosis, and therapy evaluation are presented...
  5. pmc Capillary electrophoresis-mass spectrometry as a powerful tool in biomarker discovery and clinical diagnosis: an update of recent developments
    Harald Mischak
    Mosaiques Diagnostics and Therapeutics, Hannover, Germany
    Mass Spectrom Rev 28:703-24. 2009
    ..Finally, we present several examples on the use of CE-MS to determine urinary biomarkers and implications for disease diagnosis, prognosis, and therapy evaluation...
  6. ncbi request reprint Advances in urinary proteome analysis and biomarker discovery
    Danilo Fliser
    Mosaiques Diagnostics and Therapeutics AG, Mellendorfer Strasse 7 9, D 30625 Hannover, Germany
    J Am Soc Nephrol 18:1057-71. 2007
    ..It is predicted that proteomics will play an important role in clinical nephrology in the very near future and that this progress will require interactive dialogue and collaboration between clinicians and analytical specialists...
  7. ncbi request reprint Proteomic patterns established with capillary electrophoresis and mass spectrometry for diagnostic purposes
    Eva M Weissinger
    Mosaiques Diagnostics and Therapeutics AG, Hannover, Germany
    Kidney Int 65:2426-34. 2004
    ..Proteomics applied in large scale may provide a useful diagnostic tool...
  8. ncbi request reprint Urinary proteome analysis identifies infants but not older children requiring pyeloplasty
    Jens Drube
    Department of Pediatric Kidney, Liver and Metabolic Diseases, Children s Hospital, Hannover Medical School, Hannover, Germany
    Pediatr Nephrol 25:1673-8. 2010
    ..Based on our results, the proteome pattern established by Decramer and co-workers predicts the need for surgery in infants but not in older children with UPJO...
  9. doi request reprint Quantitative urinary proteome analysis for biomarker evaluation in chronic kidney disease
    Justyna Jantos-Siwy
    Mosaiques Diagnostics and Therapeutics, Hannover, Germany
    J Proteome Res 8:268-81. 2009
    ..The calculation of absolute concentrations via exogenous stable isotope-labeled peptide standards is of no additional benefit...
  10. ncbi request reprint Urine protein patterns can serve as diagnostic tools in patients with IgA nephropathy
    Marion Haubitz
    Department of Nephrology, Hannover Medical School, Hannover, Germany
    Kidney Int 67:2313-20. 2005
    ..Early intervention and consequent therapy may prevent or at least delay the development of ESRD in these patients. Up to now, the diagnosis could only be achieved with a renal biopsy...
  11. ncbi request reprint Proteomic analysis for the assessment of diabetic renal damage in humans
    Harald Mischak
    Mosaiques Diagnostics and Therapeutics AG, Hannover, Germany
    Clin Sci (Lond) 107:485-95. 2004
    ..Longitudinal studies should explore the potential of this powerful diagnostic tool for early detection of diabetic renal damage...
  12. pmc Evaluation of the Zucker diabetic fatty (ZDF) rat as a model for human disease based on urinary peptidomic profiles
    Justyna Siwy
    Mosaiques Diagnostics, Hannover, Germany
    PLoS ONE 7:e51334. 2012
    ..Based on urinary peptidomic analysis, the ZDF rat model displays similarity to human CVD but might not be the most appropriate model to display human CKD on a molecular level...
  13. ncbi request reprint Determination of peptides and proteins in human urine with capillary electrophoresis-mass spectrometry, a suitable tool for the establishment of new diagnostic markers
    Stefan Wittke
    Mosaiques Diagnostics and Therapeutics AG, Feodor Lynen Strasse 5, 30625 Hannover, Germany
    J Chromatogr A 1013:173-81. 2003
    ..Twenty-seven polypeptides were exclusively found in samples of patients. Another 13, present in controls, were missing. These data indicate that CE-MS can be applied as powerful tool in clinical diagnostics...
  14. doi request reprint Urine proteomic analysis differentiates cholangiocarcinoma from primary sclerosing cholangitis and other benign biliary disorders
    Jochen Metzger
    Mosaiques diagnostics GmbH, Hannover, Germany
    Gut 62:122-30. 2013
    ..Nevertheless, bile collection depends on invasive endoscopic retrograde cholangiography. The authors therefore evaluated urine proteomic analysis for non-invasive CC diagnosis...
  15. ncbi request reprint Biomarker discovery by CE-MS enables sequence analysis via MS/MS with platform-independent separation
    Petra Zurbig
    Mosaiques Diagnostics and Therapeutics AG, Hannover, Germany
    Electrophoresis 27:2111-25. 2006
    ..This uniqueness facilitates the independent entry of different sequencing platforms for peptide sequencing of CE-MS-defined biomarkers from highly complex mixtures...
  16. ncbi request reprint High resolution proteome/peptidome analysis of body fluids by capillary electrophoresis coupled with MS
    Eric Schiffer
    Mosaiques Diagnostics and Therapeutics AG, Hanover, Germany
    Proteomics 6:5615-27. 2006
    ..We also discuss clinical applications of the technology for the diagnosis of renal diseases, urogenital cancer, and arteriosclerosis as well as monitoring the responses to therapeutic interventions...
  17. pmc Addressing the challenge of defining valid proteomic biomarkers and classifiers
    Mohammed Dakna
    Mosaiques Diagnostics and Therapeutics, Hannover, Germany
    BMC Bioinformatics 11:594. 2010
    ..The second urine of the morning was collected from apparently healthy male and female volunteers (aged 21-40) in the course of the routine medical check-up before recruitment at the Hannover Medical School...
  18. ncbi request reprint Capillary electrophoresis coupled to mass spectrometer for automated and robust polypeptide determination in body fluids for clinical use
    Thorsten Kaiser
    Mosaiques Diagnostics and Therapeutics AG, Hannover, Germany
    Electrophoresis 25:2044-55. 2004
    ..CE-MS detects many polypeptides in single samples and the application of the software to the search of identical polypeptides excreted in urine allows the unbiased diagnosis based on a pattern and does not rely on single disease markers...
  19. ncbi request reprint Detection of acute tubulointerstitial rejection by proteomic analysis of urinary samples in renal transplant recipients
    Stefan Wittke
    Mosaiques Diagnostics and Therapeutics AG, Hannover, Germany
    Am J Transplant 5:2479-88. 2005
    ..Further investigation is needed to establish polypeptide patterns in vascular rejection and to explore whether changes in the urinary proteome occur before the onset of histologically discernible rejection...
  20. pmc Assessment of metabolomic and proteomic biomarkers in detection and prognosis of progression of renal function in chronic kidney disease
    Esther Nkuipou-Kenfack
    Mosaiques diagnostics GmbH, Hannover, Germany Department of Toxicology, Hannover Medical School, Hannover, Germany
    PLoS ONE 9:e96955. 2014
    ..In conclusion, we found excellent association of plasma and urinary metabolites and urinary peptides with kidney function, and disease progression, but no added value in combining the different biomarkers data. ..
  21. ncbi request reprint Urine as a source for clinical proteome analysis: from discovery to clinical application
    Eva Rodríguez-Suárez
    Mosaiques diagnostics GmbH, Hannover, Germany
    Biochim Biophys Acta 1844:884-98. 2014
    ..We also discuss some current challenges in this field and reflect on future research prospects. This article is part of a Special Issue entitled: Biomarkers: A Proteomic Challenge. ..
  22. pmc The human urinary proteome reveals high similarity between kidney aging and chronic kidney disease
    Petra Zurbig
    Mosaiques Diagnostics and Therapeutics AG, Hannover, Germany
    Proteomics 9:2108-17. 2009
    ..This similarity has to be taken into account when searching for biomarkers of renal disease...
  23. doi request reprint Urinary proteomics based on capillary electrophoresis-coupled mass spectrometry in kidney disease: discovery and validation of biomarkers, and clinical application
    Harald Mischak
    Mosaiques Diagnostics and Therapeutics, Hannover, Germany
    Adv Chronic Kidney Dis 17:493-506. 2010
    ....
  24. doi request reprint Urinary excretion of twenty peptides forms an early and accurate diagnostic pattern of acute kidney injury
    Jochen Metzger
    Mosaiques diagnostics GmbH, Hannover, Germany
    Kidney Int 78:1252-62. 2010
    ....
  25. ncbi request reprint Capillary electrophoresis coupled to mass spectrometry for clinical diagnostic purposes
    Danilo Fliser
    Medical School Hannover, Hannover, Germany
    Electrophoresis 26:2708-16. 2005
    ..This review further presents examples of clinical applications of CE-MS for detection and identification of biomarkers in urine...
  26. ncbi request reprint Application of proteomics to posttransplantational follow-up
    Eva M Weissinger
    Department of Hematology, Hannover Medical School, Hannover, Germany
    Methods Mol Med 134:217-28. 2007
    ....
  27. doi request reprint Diagnosis of subclinical and clinical acute T-cell-mediated rejection in renal transplant patients by urinary proteome analysis
    Jochen Metzger
    Mosaiques diagnostics GmbH, Hannover, Germany
    Proteomics Clin Appl 5:322-33. 2011
    ..Noninvasive diagnosis of acute renal allograft rejection may be advantageous compared with the allograft biopsy...
  28. doi request reprint Clinical proteomics in obstetrics and neonatology
    Julie Klein
    Mosaiques Diagnostics and Therapeutics, Hannover, Germany
    Expert Rev Proteomics 11:75-89. 2014
    ....
  29. ncbi request reprint CE - a multifunctional application for clinical diagnosis
    Maike Sniehotta
    Mosaiques Diagnostics and Therapeutics AG, Hannover, Germany
    Electrophoresis 28:1407-17. 2007
    ..In this report, we present a technical overview of various CE techniques and discuss their applications in clinical medicine...
  30. ncbi request reprint Value of proteomics applied to the follow-up in stem cell transplantation
    Eva M Weissinger
    Department of Hematology Hemostasis Oncology, Hannover Medical School, Carl Neuberg Str 1, TPFZ KMT Ambulanz, 30625, Hannover, Germany
    Ann Hematol 85:205-11. 2006
    ..Finally, the benefits and limitations of CE-MS for the analysis of proteins in the follow up of patients after HSCT are discussed against the background of alternative technologies...
  31. ncbi request reprint Discovery and validation of urinary biomarkers for detection of renal cell carcinoma
    Maria Frantzi
    Mosaiques diagnostics GmbH, Hannover, Germany Biomedical Research Foundation, Academy of Athens, Biotechnology Division, Athens, Greece Electronic address
    J Proteomics 98:44-58. 2014
    ..This study investigates whether RCC-specific peptides with diagnostic potential can be detected in urine and whether a combination of such peptides could form a urinary screening tool...
  32. pmc Plasma and urinary amino acid metabolomic profiling in patients with different levels of kidney function
    Flore Duranton
    RD Nephrologie, Montpellier, France, Biocrates Life Sciences AG, Innsbruck, Austria, Mosaiques Diagnostics and Therapeutics AG, Hannover, Germany, Centre Hospitalier Universitaire et Université Bordeaux II, Bordeaux, France, Nephrologie, Dialyse et Transplantation, Universite de Montpellier, Hopital Lapeyronie, Centre Hospitalier Universitaire de Montpellier, Montpellier, France, Laboratoire de Recherche en Biostatistique, Epidémiologie et Recherche Clinique, Institut Universitaire de Recherche Clinique, Montpellier, France, Néphrologie Dialyse St Guilhem, Sete, France
    Clin J Am Soc Nephrol 9:37-45. 2014
    ..Patients with CKD display altered plasma amino acid profiles. This study estimated the association between the estimated GFR and urinary and plasma amino acid profiles in CKD patients...
  33. doi request reprint Exploring the uremic toxins using proteomic technologies
    Eric Schiffer
    Mosaiques Diagnostics and Therapeutics, Hanover, Germany
    Contrib Nephrol 160:159-71. 2008
    ..Subsequently, examples of the application of proteomic techniques to define novel biomarkers for renal diseases and uremic toxins will be discussed...
  34. ncbi request reprint Proteomics: a novel tool to unravel the patho-physiology of uraemia
    Eva M Weissinger
    Mosaiques Diagnostics and Therapeutics AG, Hanover, Germany
    Nephrol Dial Transplant 19:3068-77. 2004
    ....
  35. ncbi request reprint Prediction of muscle-invasive bladder cancer using urinary proteomics
    Eric Schiffer
    Mosaiques Diagnostics and Therapeutics, Hannover, Germany
    Clin Cancer Res 15:4935-43. 2009
    ..Minimally invasive methods of predicting the risk of muscle-invasive urothelial bladder carcinoma may expedite appropriate therapy and reduce morbidity and cost...
  36. ncbi request reprint Multicentre prospective validation of a urinary peptidome-based classifier for the diagnosis of type 2 diabetic nephropathy
    Justyna Siwy
    Mosaiques diagnostics GmbH, Hanover, Germany Charite Universitaetsmedizin Berlin, Medizinische Klinik IV, Berlin, Germany
    Nephrol Dial Transplant 29:1563-70. 2014
    ....
  37. doi request reprint CE-MS in biomarker discovery, validation, and clinical application
    Harald Mischak
    Mosaiques Diagnostics and Therapeutics, Hannover, Germany
    Proteomics Clin Appl 5:9-23. 2011
    ..We will present several examples of a successful application of CE-MS for biomarker discovery, implications for disease diagnosis, prognosis, and therapy evaluation, and will discuss current challenges and possible future improvements...
  38. ncbi request reprint Effects of oral vitamin C supplementation in hemodialysis patients: a proteomic assessment
    Eva M Weissinger
    Medical School of Hannover, Hannover, Germany
    Proteomics 6:993-1000. 2006
    ..These results underline the remarkable potential for proteomics to recognize specific peptide profiles in different pathological situations, which might not be detected by classical methods...
  39. doi request reprint Capillary electrophoresis-mass spectrometry in urinary proteome analysis: current applications and future developments
    Jochen Metzger
    Mosaiques Diagnostics and Therapeutics, Mellendorfer Str 7 9, 30625, Hannover, Germany
    Anal Bioanal Chem 393:1431-42. 2009
    ....
  40. ncbi request reprint Proteomics applied to the clinical follow-up of patients after allogeneic hematopoietic stem cell transplantation
    Thorsten Kaiser
    Mosaiques Diagnostics and Therapeutics and Hannover Medical School, Hannover, Germany
    Blood 104:340-9. 2004
    ..The data reveal that capillary electrophoresis and mass spectrometry allow identification of biomarkers for a variety of diseases or related complications...
  41. doi request reprint Capillary electrophoresis coupled to mass spectrometry for proteomic profiling of human urine and biomarker discovery
    Petra Zurbig
    Mosaiques diagnostics GmbH, Mellendorfer Str 7 9, 30625 Hannover, Germany
    Methods Mol Biol 564:105-21. 2009
    ..In this chapter, we discuss materials and methods for CE-MS-based clinical proteomics allowing the reproducible profiling of urine...
  42. doi request reprint Capillary electrophoresis coupled to mass spectrometry for biomarker discovery and diagnosis of kidney diseases
    Petra Zurbig
    Mosaiques Diagnostics and Therapeutics AG, Hannover, Germany
    Contrib Nephrol 160:107-26. 2008
    ..This advancement offers the early diagnosis of different nephrological diseases...
  43. doi request reprint Technical, bioinformatical and statistical aspects of liquid chromatography-mass spectrometry (LC-MS) and capillary electrophoresis-mass spectrometry (CE-MS) based clinical proteomics: a critical assessment
    Mohammed Dakna
    Mosaiques Diagnostics and Therapeutics, Hannover, Germany
    J Chromatogr B Analyt Technol Biomed Life Sci 877:1250-8. 2009
    ..We further review the statistical issues of feature selection and sample classification...
  44. pmc Urinary proteomics for early diagnosis in diabetic nephropathy
    Petra Zurbig
    Mosaiques diagnostics GmbH, Hannover, Germany
    Diabetes 61:3304-13. 2012
    ..Before albumin excretion starts to increase, there is a decrease in collagen fragments. Urinary proteomics enables noninvasive assessment of DN risk at an early stage via determination of specific collagen fragments...
  45. ncbi request reprint Discovery of biomarkers in human urine and cerebrospinal fluid by capillary electrophoresis coupled to mass spectrometry: towards new diagnostic and therapeutic approaches
    Stefan Wittke
    Mosaiques Diagnostics and Therapeutics AG, Hannover, Germany
    Electrophoresis 26:1476-87. 2005
    ..1000 individual native polypeptides within ca. 60 min...
  46. ncbi request reprint Differential polypeptide display: the search for the elusive target
    Stefan Wittke
    Mosaiques Diagnostics and Therapeutics AG, Hannover, Germany
    J Chromatogr B Analyt Technol Biomed Life Sci 803:17-26. 2004
    ..The developments of the last years are presented discussed...
  47. doi request reprint Bile proteomic profiles differentiate cholangiocarcinoma from primary sclerosing cholangitis and choledocholithiasis
    Tim O Lankisch
    Department of Gastroenterology, Hepatology and Endocrinology, Hannover Medical School, Hannover, Germany
    Hepatology 53:875-84. 2011
    ..87 (95% CI: 0.73-0.95, P = 0.0001) in ROC analysis. Eight out of 10 samples of patients with CC complicating PSC were identified...
  48. doi request reprint Urinary proteome pattern in children with renal Fanconi syndrome
    Jens Drube
    Department of Paediatric Nephrology, Children s Hospital, Hannover Medical School, Hannover, Germany
    Nephrol Dial Transplant 24:2161-9. 2009
    ..We studied the urinary low-molecular-weight proteome to identify excreted peptides indicative of a pathogenetic mechanism leading to tubular dysfunction...
  49. pmc Comprehensive human urine standards for comparability and standardization in clinical proteome analysis
    Harald Mischak
    Mosaiques Diagnostics and Therapeutics AG, Hannover, Germany
    Proteomics Clin Appl 4:464-78. 2010
    ..Urine proteomics is emerging as a powerful tool for biomarker discovery. The purpose of this study is the development of a well-characterized "real life" sample that can be used as reference standard in urine clinical proteomics studies...
  50. ncbi request reprint Comparison of CE-MS/MS and LC-MS/MS sequencing demonstrates significant complementarity in natural peptide identification in human urine
    Julie Klein
    Mosaiques Diagnostics, Hannover, Germany
    Electrophoresis 35:1060-4. 2014
    ..The combination of both technologies results in significantly increased sequence coverage...
  51. doi request reprint Challenges of using mass spectrometry as a bladder cancer biomarker discovery platform
    Eric Schiffer
    Mosaiques Diagnostics and Therapeutics AG, Hannover, Germany
    World J Urol 26:67-74. 2008
    ..In consequence, the necessity of repeated screening for reappearance demonstrates the urgent need for novel biomarkers as alternatives to invasive standard procedures...
  52. doi request reprint Markers of vascular disease in plasma from patients with chronic kidney disease identified by proteomic analysis
    Eric Schiffer
    Mosaiques diagnostics GmbH, Hannover, Germany
    J Hypertens 29:783-90. 2011
    ..Arterial calcification and aortic stiffness are currently used as surrogates for vascular alterations. However, still little is known about prediction and the patho-physiologic mechanisms leading to CVD...
  53. ncbi request reprint Capillary electrophoresis coupled to mass spectrometry to establish polypeptide patterns in dialysis fluids
    Thorsten Kaiser
    Mosaiques Diagnostics and Therapeutics AG, Feodor Lynen Strasse 5, 30625 Hannover, Germany
    J Chromatogr A 1013:157-71. 2003
    ..We established a fast and sensitive technique, easily applicable to the monitoring of different modalities of dialyzers...