Gerardo Gamba


Country: Mexico


  1. Gamba G. WNK lies upstream of kinases involved in regulation of ion transporters. Biochem J. 2005;391:e1-3 pubmed
    ..The phosphorylation site involved in SPAK or OSR1 activation is identified as a threonine residue within the T-loop. ..
  2. Bazúa Valenti S, Rojas Vega L, Castañeda Bueno M, Barrera Chimal J, Bautista R, Cervantes Pérez L, et al. The Calcium-Sensing Receptor Increases Activity of the Renal NCC through the WNK4-SPAK Pathway. J Am Soc Nephrol. 2018;29:1838-1848 pubmed publisher
    ..It is possible that activation of CaSR by Ca2+ in the apical membrane of the DCT increases NaCl reabsorption by NCC, with the consequent, well known decrease of Ca2+ reabsorption, further promoting hypercalciuria. ..
  3. Bazúa Valenti S, Gamba G. Revisiting the NaCl cotransporter regulation by with-no-lysine kinases. Am J Physiol Cell Physiol. 2015;308:C779-91 pubmed publisher
    ..The understanding of the effect of WNKs on NCC is a complex issue, but recent evidence discussed in this review suggests that we could be reaching the end of the dark ages regarding this matter. ..
  4. Rojas Vega L, Jiménez Vega A, Bazúa Valenti S, Arroyo Garza I, Jiménez J, Gómez Ocádiz R, et al. Increased phosphorylation of the renal Na+-Cl- cotransporter in male kidney transplant recipient patients with hypertension: a prospective cohort. Am J Physiol Renal Physiol. 2015;309:F836-42 pubmed publisher
    ..Our results are consistent with the hypothesis that NCC activation plays a major role in tacrolimus-induced hypertension. ..
  5. Bazúa Valenti S, Chávez Canales M, Rojas Vega L, González Rodríguez X, Vázquez N, Rodríguez Gama A, et al. The Effect of WNK4 on the Na+-Cl- Cotransporter Is Modulated by Intracellular Chloride. J Am Soc Nephrol. 2015;26:1781-6 pubmed publisher
    ..These observations suggest that WNK4 can exert differential effects on NCC, depending on the intracellular chloride concentration. ..
  6. Rojas Vega L, Gamba G. Mini-review: regulation of the renal NaCl cotransporter by hormones. Am J Physiol Renal Physiol. 2016;310:F10-4 pubmed publisher
  7. Gamba G. Regulation of the renal Na+-Cl- cotransporter by phosphorylation and ubiquitylation. Am J Physiol Renal Physiol. 2012;303:F1573-83 pubmed publisher
    ..This work presents a review of our current knowledge of the regulation of NCC activity by phosphorylation and ubiquitylation. ..
  8. Hadchouel J, Ellison D, Gamba G. Regulation of Renal Electrolyte Transport by WNK and SPAK-OSR1 Kinases. Annu Rev Physiol. 2016;78:367-89 pubmed publisher
    ..In contrast, the process of regulating the channels is independent of SPAK-OSR1. We present a review of the remarkable advances in this area in the past 10 years. ..
  9. Barba Navarro R, Tapia Silva M, Garza García C, Lopez Giacoman S, Melgoza Toral I, Vazquez Rangel A, et al. The Effect of Spironolactone on Acute Kidney Injury After Cardiac Surgery: A Randomized, Placebo-Controlled Trial. Am J Kidney Dis. 2017;69:192-199 pubmed publisher
    ..Our trial demonstrated that spironolactone was not protective for AKI associated with cardiac surgery and there may be a trend toward risk. ..

More Information


  1. Moreno E, Plata C, Rodríguez Gama A, Argaiz E, Vazquez N, Leyva Ríos K, et al. The European Eel NCC? Gene Encodes a Thiazide-resistant Na-Cl Cotransporter. J Biol Chem. 2016;291:22472-22481 pubmed
    ..Thus, NCC? exhibits significant functional differences from NCCs that could be helpful in defining several aspects of the structure-function relationship of this important cotransporter. ..
  2. Gamba G. Molecular physiology and pathophysiology of electroneutral cation-chloride cotransporters. Physiol Rev. 2005;85:423-93 pubmed
  3. Gamba G. The thiazide-sensitive Na+-Cl- cotransporter: molecular biology, functional properties, and regulation by WNKs. Am J Physiol Renal Physiol. 2009;297:F838-48 pubmed publisher
    ..Thus one of the legacies of Steve Hebert is a better understanding of salt and water homeostasis. ..
  4. Bazúa Valenti S, Castañeda Bueno M, Gamba G. Physiological role of SLC12 family members in the kidney. Am J Physiol Renal Physiol. 2016;311:F131-44 pubmed publisher
    ..In this work, we present an overview of the physiological roles of these transporters in the kidney, with particular emphasis on the knowledge gained in the past few years. ..
  5. Mercado A, de los Heros P, Melo Z, Chavez Canales M, Murillo de Ozores A, Moreno E, et al. With no lysine L-WNK1 isoforms are negative regulators of the K+-Cl- cotransporters. Am J Physiol Cell Physiol. 2016;311:C54-66 pubmed publisher
    ..Together, our data support that NKCC1 and KCCs are coordinately regulated by L-WNK1 isoforms. ..
  6. Melo Z, Cruz Rangel S, Bautista R, Vazquez N, Castañeda Bueno M, Mount D, et al. Molecular evidence for a role for K(+)-Cl(-) cotransporters in the kidney. Am J Physiol Renal Physiol. 2013;305:F1402-11 pubmed publisher
    ..Our data are consistent with a role for KCC3 in the proximal tubule glucose reabsorption mechanism and for KCC4 in salt reabsorption of the thick ascending loop of Henle's loop and acid secretion of the collecting duct. ..
  7. Gamba G. Regulation of NKCC2 activity by SPAK truncated isoforms. Am J Physiol Renal Physiol. 2014;306:F49-50 pubmed publisher
  8. Castañeda Bueno M, Cervantes Pérez L, Rojas Vega L, Arroyo Garza I, Vazquez N, Moreno E, et al. Modulation of NCC activity by low and high K(+) intake: insights into the signaling pathways involved. Am J Physiol Renal Physiol. 2014;306:F1507-19 pubmed publisher
    ..Thus, the accompanying anion might modulate the NCC response to the high-K(+) diet. ..
  9. Lagnaz D, Arroyo J, Chavez Canales M, Vazquez N, Rizzo F, Spirlì A, et al. WNK3 abrogates the NEDD4-2-mediated inhibition of the renal Na+-Cl- cotransporter. Am J Physiol Renal Physiol. 2014;307:F275-86 pubmed publisher
    ..Together, these results suggest that a novel role for WNK3 on NCC expression at the plasma membrane, an effect apparently independent of the SPAK kinase and the aldosterone-SGK1 pathway. ..
  10. Rojas Vega L, Reyes Castro L, Ramírez V, Bautista Pérez R, Rafael C, Castañeda Bueno M, et al. Ovarian hormones and prolactin increase renal NaCl cotransporter phosphorylation. Am J Physiol Renal Physiol. 2015;308:F799-808 pubmed publisher
    ..Our results indicate that estradiol, progesterone, and prolactin, the hormones that are involved in sexual cycle, pregnancy and lactation, upregulate the activity of NCC. ..
  11. Gamba G, Friedman P. Thick ascending limb: the Na(+):K (+):2Cl (-) co-transporter, NKCC2, and the calcium-sensing receptor, CaSR. Pflugers Arch. 2009;458:61-76 pubmed publisher
    ..The present review discusses these functions and their genetic and molecular regulation...
  12. Torre Villalvazo I, Cervantes Pérez L, Noriega L, Jimenez J, Uribe N, Chavez Canales M, et al. Inactivation of SPAK kinase reduces body weight gain in mice fed a High-fat diet by improving energy expenditure and insulin sensitivity. Am J Physiol Endocrinol Metab. 2017;:ajpendo.00108.2017 pubmed publisher