Patrick M McDonough

Summary

Publications

  1. pmc Quantification of lipid droplets and associated proteins in cellular models of obesity via high-content/high-throughput microscopy and automated image analysis
    Patrick M McDonough
    Vala Sciences Inc, San Diego, California 92109, USA
    Assay Drug Dev Technol 7:440-60. 2009
  2. pmc Differential phosphorylation of perilipin 1A at the initiation of lipolysis revealed by novel monoclonal antibodies and high content analysis
    Patrick M McDonough
    Vala Sciences Inc, San Diego, California, United States of America
    PLoS ONE 8:e55511. 2013
  3. pmc Quantification of hormone sensitive lipase phosphorylation and colocalization with lipid droplets in murine 3T3L1 and human subcutaneous adipocytes via automated digital microscopy and high-content analysis
    Patrick M McDonough
    Vala Sciences, Inc, San Diego, California, USA
    Assay Drug Dev Technol 9:262-80. 2011
  4. ncbi request reprint A live cell, image-based approach to understanding the enzymology and pharmacology of 2-bromopalmitate and palmitoylation
    Ivana Mikic
    Vala Sciences Inc, La Jolla, CA, USA
    Methods Enzymol 414:150-87. 2006
  5. ncbi request reprint Plasma membrane assays and three-compartment image cytometry for high content screening
    Natalie L Prigozhina
    Vala Sciences, Inc, San Diego, CA 92121, USA
    Assay Drug Dev Technol 5:29-48. 2007
  6. doi request reprint Identification of MicroRNAs that control lipid droplet formation and growth in hepatocytes via high-content screening
    Ross Whittaker
    Vala Sciences Inc, San Diego, CA, USA
    J Biomol Screen 15:798-805. 2010
  7. ncbi request reprint Deletion of the inducible 70-kDa heat shock protein genes in mice impairs cardiac contractile function and calcium handling associated with hypertrophy
    Yun Kyung Kim
    Department of Medicine, University of California, San Diego, La Jolla, CA 92093 0618, USA
    Circulation 113:2589-97. 2006
  8. ncbi request reprint The antidiabetic agent rosiglitazone upregulates SERCA2 and enhances TNF-alpha- and LPS-induced NF-kappaB-dependent transcription and TNF-alpha-induced IL-6 secretion in ventricular myocytes
    Rajanya D Shah
    The Rees Stealy Research Foundation Laboratory, San Diego, USA
    Cell Physiol Biochem 15:41-50. 2005
  9. ncbi request reprint A dynamic pool of calcium in catecholamine storage vesicles. Exploration in living cells by a novel vesicle-targeted chromogranin A-aequorin chimeric photoprotein
    Nitish R Mahapatra
    Department of Medicine, University of California, San Diego, La Jolla 92093 0838, USA
    J Biol Chem 279:51107-21. 2004
  10. pmc Sorcin modulates mitochondrial Ca(2+) handling and reduces apoptosis in neonatal rat cardiac myocytes
    Jorge Suarez
    Department of Medicine, University of California, San Diego, La Jolla, CA 92093, USA
    Am J Physiol Cell Physiol 304:C248-56. 2013

Collaborators

  • Jeffrey H Price
  • Ying Hu
  • Jun Zhang
  • Nitish R Mahapatra
  • Eric Swanson
  • Darrell D Belke
  • Hong Wang
  • DANIEL T O'CONNOR
  • Sean M Hartig
  • Jorge Suarez
  • Ross Whittaker
  • Wolfgang H Dillmann
  • Ivana Mikic
  • Fabio Cerignoli
  • Natalie L Prigozhina
  • Scott Callaway
  • Yun Kyung Kim
  • Rajanya D Shah
  • Markus Meyer
  • Brian T Scott
  • Angelica Suarez-Ramirez
  • Eduardo S Fricovsky
  • David Charlot
  • Piyush Gehalot
  • Mark Mercola
  • Randy Ingermanson
  • Rob Towart
  • David J Gallacher
  • Alex Savchenko
  • Patricia A Loy
  • Eugene Sisman
  • Eigo Suyama
  • Pedro Aza-Blanc
  • Randall S Ingermanson
  • Dennis R Roop
  • Ling Zhong
  • Michael A Mancini
  • Edward A Hunter
  • David A Zacharias
  • David J Dix
  • Sonia Planey
  • Terri Seron
  • Rachael Watson
  • Benedikt von Massenbach
  • Edward Hunter
  • Carolina Ceballos
  • David Zacharias
  • Christa Boer
  • Edwin Golez
  • Frank Gonzales
  • Ami Abbott
  • Shawna Caudillo
  • John Morello
  • Paul J Paolini
  • Huda E Shubeita
  • Danielle Augustin
  • Wolfgang F Bluhm
  • Susanne U Trost
  • Brian Scott
  • Peter Ho
  • Stephen P Cary
  • Thomas Dieterle
  • Gregg J Silverman

Detail Information

Publications12

  1. pmc Quantification of lipid droplets and associated proteins in cellular models of obesity via high-content/high-throughput microscopy and automated image analysis
    Patrick M McDonough
    Vala Sciences Inc, San Diego, California 92109, USA
    Assay Drug Dev Technol 7:440-60. 2009
    ..The results suggest that HCA techniques can be utilized to quantify lipid droplets and associated proteins in many cell models relevant to a variety of diseases...
  2. pmc Differential phosphorylation of perilipin 1A at the initiation of lipolysis revealed by novel monoclonal antibodies and high content analysis
    Patrick M McDonough
    Vala Sciences Inc, San Diego, California, United States of America
    PLoS ONE 8:e55511. 2013
    ..Thus, we suggest perilipin 1A and HSL are differentially phosphorylated in a similar manner at the initiation of lipolysis and arginine residues near the target serines may influence this process...
  3. pmc Quantification of hormone sensitive lipase phosphorylation and colocalization with lipid droplets in murine 3T3L1 and human subcutaneous adipocytes via automated digital microscopy and high-content analysis
    Patrick M McDonough
    Vala Sciences, Inc, San Diego, California, USA
    Assay Drug Dev Technol 9:262-80. 2011
    ..50. The results illustrate several key differences between human and murine adipocytes and demonstrate advantages of utilizing HCA techniques to study lipolysis in cultured adipocytes...
  4. ncbi request reprint A live cell, image-based approach to understanding the enzymology and pharmacology of 2-bromopalmitate and palmitoylation
    Ivana Mikic
    Vala Sciences Inc, La Jolla, CA, USA
    Methods Enzymol 414:150-87. 2006
    ..Using this system we determined the residence half-life of palmitate on the dipalmitoyl substrate peptide from GAP43 as well as the EC(50) for 2-bromopalmitate, a common inhibitor of palmitoylation...
  5. ncbi request reprint Plasma membrane assays and three-compartment image cytometry for high content screening
    Natalie L Prigozhina
    Vala Sciences, Inc, San Diego, CA 92121, USA
    Assay Drug Dev Technol 5:29-48. 2007
    ..The results demonstrate that this new algorithm for plasma membrane image cytometry enables statistically significant analyses in a variety of applications in both cultured cells and tissue sections...
  6. doi request reprint Identification of MicroRNAs that control lipid droplet formation and growth in hepatocytes via high-content screening
    Ross Whittaker
    Vala Sciences Inc, San Diego, CA, USA
    J Biomol Screen 15:798-805. 2010
    ..Thus, HCA-based screening identified novel miRNA and protein regulators of LDs and cholesterol metabolism that may be relevant to hepatic diseases arising from obesity and alcohol abuse...
  7. ncbi request reprint Deletion of the inducible 70-kDa heat shock protein genes in mice impairs cardiac contractile function and calcium handling associated with hypertrophy
    Yun Kyung Kim
    Department of Medicine, University of California, San Diego, La Jolla, CA 92093 0618, USA
    Circulation 113:2589-97. 2006
    ..Hspa1a and Hspa1b genes encode stress-inducible 70-kDa heat shock proteins (Hsp70) that protect cells from insults such as ischemia. Mice with null mutations of both genes (KO) were generated, and their cardiac phenotype was explored...
  8. ncbi request reprint The antidiabetic agent rosiglitazone upregulates SERCA2 and enhances TNF-alpha- and LPS-induced NF-kappaB-dependent transcription and TNF-alpha-induced IL-6 secretion in ventricular myocytes
    Rajanya D Shah
    The Rees Stealy Research Foundation Laboratory, San Diego, USA
    Cell Physiol Biochem 15:41-50. 2005
    ..5-fold. Rosiglitazone had no significant effects on basal levels of NF-kappaB-dependent transcription and IL-6 secretion. Thus, cardioprotective effects of rosiglitazone may be partly mediated by NF-kappaB...
  9. ncbi request reprint A dynamic pool of calcium in catecholamine storage vesicles. Exploration in living cells by a novel vesicle-targeted chromogranin A-aequorin chimeric photoprotein
    Nitish R Mahapatra
    Department of Medicine, University of California, San Diego, La Jolla 92093 0838, USA
    J Biol Chem 279:51107-21. 2004
    ....
  10. pmc Sorcin modulates mitochondrial Ca(2+) handling and reduces apoptosis in neonatal rat cardiac myocytes
    Jorge Suarez
    Department of Medicine, University of California, San Diego, La Jolla, CA 92093, USA
    Am J Physiol Cell Physiol 304:C248-56. 2013
    ..In contrast, antisense sorcin induced caspase-3 activation. Thus, sorcin antiapoptotic properties may be due to modulation of mitochondrial Ca(2+) handling in cardiac myocytes...
  11. pmc High throughput measurement of Ca²⁺ dynamics for drug risk assessment in human stem cell-derived cardiomyocytes by kinetic image cytometry
    Fabio Cerignoli
    Sanford Burnham Medical Research Institute, 10901N Torrey Pines Road, La Jolla, CA 92037, USA
    J Pharmacol Toxicol Methods 66:246-56. 2012
    ....
  12. ncbi request reprint A recombinant antibody increases cardiac contractility by mimicking phospholamban phosphorylation
    Markus Meyer
    Department of Medicine, University of California, San Diego, La Jolla, CA 92093 0618, USA
    FASEB J 18:1312-4. 2004
    ..The PLN-Ab provides a novel therapeutic approach to improving contractility through in vivo expression of an antibody inside cardiac myocytes...

Research Grants9

  1. HT Image Assay of Lipid Droplet Formation in Human Adipocytes
    Patrick McDonough; Fiscal Year: 2006
    ..Phase 2 will likely involve further development of the software and staining techniques (particularly with regard to the proteins) and expansion of the product line to include additional cell types and other proteins of interest. ..
  2. Development automated assay-regulators insulin synthesis
    Patrick McDonough; Fiscal Year: 2006
    ..Such bioactive molecules will be used subsequently to probe the regulatory pathways that control insulin secretion and should contribute to cell-based or pharmacologic treatments for type-I and -II diabetes. ..
  3. High Throughput Imaging Assay for Beta-Catenin
    Patrick McDonough; Fiscal Year: 2007
    ..Discovery of such chemicals will contribute to our understanding of the regulation of ¿-catenin localization and potentially help lead to development of novel therapeutics, depression, and dementia. ..
  4. Live cell and HCS assays to quantify production of cardiomyocytes from stem cells
    Patrick McDonough; Fiscal Year: 2007
    ..We will develop an instrument that will help researchers produce heart cells from embryonic stem cells. Heart cells produced in this manner can potentially be used to treat heart disease. ..