Mahiran Basri

Summary

Affiliation: Universiti Putra Malaysia
Country: Malaysia

Publications

  1. doi request reprint Optimized lipase-catalyzed synthesis of adipate ester in a solvent-free system
    Naz Chaibakhsh
    Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor Darul Ehsan, Malaysia
    J Ind Microbiol Biotechnol 36:1149-55. 2009
  2. pmc A potential tocopherol acetate loaded palm oil esters-in-water nanoemulsions for nanocosmeceuticals
    Brian Sheng Xian Teo
    Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia
    J Nanobiotechnology 8:4. 2010
  3. doi request reprint Molten globule-triggered inactivation of a thermostable and solvent stable lipase in hydrophilic solvents
    Tengku Haziyamin Tengku Abdul Hamid
    Department of Microbiology, University Putra Malaysia, Serdang, Selangor, Malaysia
    Protein J 29:290-7. 2010
  4. pmc Molecular dynamics simulation of palmitate ester self-assembly with diclofenac
    Roghayeh Abedi Karjiban
    Department of Chemistry, Faculty of Science, UPM Serdang, Universiti Putra Malaysia, Selangor 43400, Malaysia E Mails M B M B A R
    Int J Mol Sci 13:9572-83. 2012
  5. pmc Comparison of estimation capabilities of response surface methodology (RSM) with artificial neural network (ANN) in lipase-catalyzed synthesis of palm-based wax ester
    Mahiran Basri
    Faculty of Science, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia
    BMC Biotechnol 7:53. 2007
  6. doi request reprint Secretory expression and characterization of a highly Ca2+-activated thermostable L2 lipase
    Suriana Sabri
    Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia
    Protein Expr Purif 68:161-6. 2009
  7. doi request reprint High level expression and characterization of a novel thermostable, organic solvent tolerant, 1,3-regioselective lipase from Geobacillus sp. strain ARM
    Afshin Ebrahimpour
    Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia
    Bioresour Technol 102:6972-81. 2011
  8. doi request reprint Purification and characterisation of an F16L mutant of a thermostable lipase
    Mohd Shukuri Mohamad Ali
    Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia
    Protein J 31:229-37. 2012
  9. doi request reprint Molecular investigation of a gene encoding organic solvent-tolerant alkaline protease from Pseudomonas aeruginosa strain K
    Raja Noor Zaliha Raja Abd Rahman
    Enzyme and Microbial Technology Laboratory, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang, Selangor, Malaysia
    J Basic Microbiol 50:143-9. 2010
  10. ncbi request reprint A new organic solvent tolerant protease from Bacillus pumilus 115b
    Raja Noor Zaliha Raja Abd Rahman
    Enzyme and Microbial Technology Research, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, UPM Serdang, 43400 Selangor, Malaysia
    J Ind Microbiol Biotechnol 34:509-17. 2007

Collaborators

  • Hiroyoshi Matsumura
  • Afshin Ebrahimpour
  • Raja Noor Zaliha Raja Abd Rahman
  • Donald Jacobs
  • Suraini Abd-Aziz
  • Arbakariya B Ariff
  • Mohd Zobir Hussein
  • Nursyamsyila Mat Hadzir
  • Mohd Basyaruddin Abd Rahman
  • Yasushi Kai
  • Bimo A Tejo
  • Abu Bakar Salleh
  • Mohd Basyaruddin Abdul Rahman
  • Mohd Shukuri Mohamad Ali
  • Thean Chor Leow
  • Roghayeh Abedi Karjiban
  • Raja Noor Zaliha Raja Abd Rahman
  • Naz Chaibakhsh
  • Fairolniza Mohd Shariff
  • Adam Leow Thean Chor
  • Raja Noor Zaliha Raja Abdul Rahman
  • Roswanira Abdul Wahab
  • Emilia Abdulmalek
  • Hamid Reza Fard Masoumi
  • Siti Nurbaya Oslan
  • Anuar Kassim
  • Amaliawati Ahmad Latiffi
  • Menega Ganasen
  • Chaw Jiang Lim
  • Rudzanna Ruslan
  • Mohd Zulhilmi Abdul Rahman
  • Syafinaz Zainol
  • Ng Sook Han
  • Rubina Nelofer
  • Norazlinaliza Salim
  • Syed Hussinien H Shah
  • Dzulkifly Kuang Abdullah
  • Iffah Izzati Zakaria
  • Zahariah Ismail
  • Loo Chew Hung
  • Uswatun Hasanah Zaidan
  • Mohd Saif Khusaini
  • Kok Whye Cheong
  • Atena Adnani
  • Nur Fariza Abd Rahman
  • Chee Fah Wong
  • Tengku Haziyamin Tengku Abdul Hamid
  • Brian Sheng Xian Teo
  • Salina Mat Radzi
  • Faujan Bin H Ahmad
  • Siti Salwa Abd Gani
  • Rosfarizan Mohamad
  • Randa A Abusham
  • Suriana Sabri
  • Siti Efliza Ashari
  • Habibah Abdul Wahab
  • Moohamad Ropaning Sulong
  • Zhibiao Fu
  • Dzolkhifli Omar
  • Siti Farhanie Mohd Fuzi
  • Zatty Syamimi Adura Mat Said
  • Mahashanon Arumugam
  • Ahmad Fuad Shamsuddin
  • Anirban Bhunia
  • Chong Chai Yun
  • Mohd Basyaruddin Abd Rahman
  • Emilia Abdul-Malek
  • Hamidon Basri
  • Subhrangsu Chatterjee
  • Nor M Mahadi
  • Rajiv K Kar
  • Abu B Salleh
  • Dzulkefly K Abdullah
  • Hamidon Bin Basri
  • Leow Thean Chor
  • Abdul Munir A Murad
  • Ramakrishnan Nagasundara Ramanan
  • Azren A Asmawi
  • Siti Salwa Abdul-Gani
  • Choo Yuen May
  • Hazimah Abu Hassan
  • Mohd Jelas Haron
  • Rosnah Ismail
  • Harrison Lau Lik Nang
  • Nor Izuan Jarmi
  • Siti Salhah Othman
  • Hossein Abbastabar Ahangar
  • Raja Noor Zaliha
  • Thean Chor
  • Adam Leow

Detail Information

Publications72

  1. doi request reprint Optimized lipase-catalyzed synthesis of adipate ester in a solvent-free system
    Naz Chaibakhsh
    Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor Darul Ehsan, Malaysia
    J Ind Microbiol Biotechnol 36:1149-55. 2009
    ..Higher volumetric productivity compared to a solvent-based system was also offered by SFS. The results demonstrate that the solvent-free system is efficient for enzymatic synthesis of adipate ester...
  2. pmc A potential tocopherol acetate loaded palm oil esters-in-water nanoemulsions for nanocosmeceuticals
    Brian Sheng Xian Teo
    Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia
    J Nanobiotechnology 8:4. 2010
    ..abstract:..
  3. doi request reprint Molten globule-triggered inactivation of a thermostable and solvent stable lipase in hydrophilic solvents
    Tengku Haziyamin Tengku Abdul Hamid
    Department of Microbiology, University Putra Malaysia, Serdang, Selangor, Malaysia
    Protein J 29:290-7. 2010
    ..The H-bonding arrangements triggered in methanol and DMSO, respectively, caused different forms of tertiary structure perturbations on Lip 42, despite both showing partial denaturation with molten globule formations...
  4. pmc Molecular dynamics simulation of palmitate ester self-assembly with diclofenac
    Roghayeh Abedi Karjiban
    Department of Chemistry, Faculty of Science, UPM Serdang, Universiti Putra Malaysia, Selangor 43400, Malaysia E Mails M B M B A R
    Int J Mol Sci 13:9572-83. 2012
    ..Both micelles showed a prolate-like shape, while adding the drug produced a more compact micellar structure. Our results proposed that the drug could behave as a co-surfactant in our simulated model...
  5. pmc Comparison of estimation capabilities of response surface methodology (RSM) with artificial neural network (ANN) in lipase-catalyzed synthesis of palm-based wax ester
    Mahiran Basri
    Faculty of Science, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia
    BMC Biotechnol 7:53. 2007
    ..RSM is one of the most popular techniques used for optimization of chemical and biochemical processes and ANNs are powerful and flexible tools that are well suited to modeling biochemical processes...
  6. doi request reprint Secretory expression and characterization of a highly Ca2+-activated thermostable L2 lipase
    Suriana Sabri
    Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia
    Protein Expr Purif 68:161-6. 2009
    ..Stabilization at high temperature and alkaline pH as well as its broad substrate specificity offer great potential for application in various industries that require high temperature operations...
  7. doi request reprint High level expression and characterization of a novel thermostable, organic solvent tolerant, 1,3-regioselective lipase from Geobacillus sp. strain ARM
    Afshin Ebrahimpour
    Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia
    Bioresour Technol 102:6972-81. 2011
    ..ARM lipase was active in a broad range of temperature and pH with optimum lipolytic activity at pH 8.0 and 65°C. The enzyme retained 50% residual activity at pH 6.0-7.0, 50°C for more than 150 min...
  8. doi request reprint Purification and characterisation of an F16L mutant of a thermostable lipase
    Mohd Shukuri Mohamad Ali
    Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia
    Protein J 31:229-37. 2012
    ..Further, the activity was not impaired by water-miscible solvents, including methanol, ethanol, and acetone...
  9. doi request reprint Molecular investigation of a gene encoding organic solvent-tolerant alkaline protease from Pseudomonas aeruginosa strain K
    Raja Noor Zaliha Raja Abd Rahman
    Enzyme and Microbial Technology Laboratory, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang, Selangor, Malaysia
    J Basic Microbiol 50:143-9. 2010
    ..coli BL21 (DE3) with an optimum proteolytic activity detected at 1.0112 U/ml. To date, this is the first reported expression of alkaline protease (aprA) with such remarkable property in Escherichia coli...
  10. ncbi request reprint A new organic solvent tolerant protease from Bacillus pumilus 115b
    Raja Noor Zaliha Raja Abd Rahman
    Enzyme and Microbial Technology Research, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, UPM Serdang, 43400 Selangor, Malaysia
    J Ind Microbiol Biotechnol 34:509-17. 2007
    ..This is the only report available to date on organic solvent tolerant protease from B. pumilus...
  11. ncbi request reprint Kinetic behaviour of free lipase and mica-based immobilized lipase catalyzing the synthesis of sugar esters
    Uswatun Hasanah Zaidan
    Department of Chemistry, Faculty of Science, Universiti Putra Malaysia
    Biosci Biotechnol Biochem 75:1446-50. 2011
    ..The kinetic parameters deduced from this model were used to simulate reaction rate data which were in close agreement with the experimental values...
  12. doi request reprint Lipase-catalyzed dimethyl adipate synthesis: response surface modeling and kinetics
    Naz Chaibakhsh
    Department of Chemistry, Universiti Putra Malaysia, UPM Serdang, Selangor Darul Ehsan, Malaysia
    Biotechnol J 5:848-55. 2010
    ..The kinetic parameters were determined and used to simulate the experimental results. A good quality of fit was observed between the simulated and experimental initial rates...
  13. doi request reprint Manipulation of the conformation and enzymatic properties of T1 lipase by site-directed mutagenesis of the protein core
    Roswanira Abdul Wahab
    Faculty of Science, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia
    Appl Biochem Biotechnol 167:612-20. 2012
    ..This is especially true when the residue is next to the catalytic serine as demonstrated with the Phe and Trp mutation...
  14. ncbi request reprint Kinetics of enzymatic synthesis of liquid wax ester from oleic acid and oleyl alcohol
    Salina Mat Radzi
    Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia
    J Oleo Sci 59:127-34. 2010
    ..Activation energy shows a value of 21.77 Kcal/mol. The thermodynamic parameters of the process, enthalpy and entropy, were 21.15 Kcal/mol and 52.07 cal/mol.K, respectively...
  15. doi request reprint Organic-solvent stability of elastase strain K overexpressed in an Escherichia-Pseudomonas expression system
    Chee Fah Wong
    Enzyme and Microbial Technology Laboratory, Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia
    Biotechnol Appl Biochem 57:1-7. 2010
    ....
  16. doi request reprint Chemometric analysis of lipase-catalyzed synthesis of xylitol esters in a solvent-free system
    Atena Adnani
    Faculty of Science, Universiti Putra Malaysia, Serdang, Selangor, Malaysia
    Carbohydr Res 346:472-9. 2011
    ..5%) shows that the model derived from the Taguchi orthogonal array can be used for optimization and better understanding of the effect of reaction parameters on the enzymatic synthesis of xylitol esters in a solvent-free system...
  17. ncbi request reprint Deciphering the flexibility and dynamics of Geobacillus zalihae strain T1 lipase at high temperatures by molecular dynamics simulation
    Mohd Basyaruddin Abdul Rahman
    Structural Biology Research Centre, Malaysia Genome Institute, 43600 UKM Bangi, Selangor, Malaysia
    Protein Pept Lett 16:1360-70. 2009
    ..The results suggest that the small domain of model enzyme is a critical region to the thermostability of this organism...
  18. pmc Unlocking the mystery behind the activation phenomenon of T1 lipase: a molecular dynamics simulations approach
    Mohd Zulhilmi Abdul Rahman
    Institute of Bioscience, Universiti Putra Malaysia, 43400 Serdang, Selangor
    Protein Sci 21:1210-21. 2012
    ....
  19. ncbi request reprint Locally isolated yeasts from Malaysia: identification, phylogenetic study and characterization
    Siti Nurbaya Oslan
    Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang, Selangor, Malaysia
    Acta Biochim Pol 59:225-9. 2012
    ..In conclusion, a new yeast-vector system for isolate WB can be developed by using phleomycin or geneticin as the drugs resistance marker. Moreover, strains RT and R1 can be investigated as a novel source of a thermostable lipase...
  20. doi request reprint Modeling and optimization of lipase-catalyzed production of succinic acid ester using central composite design analysis
    Mohd Basyaruddin Abdul Rahman
    Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor Darul Ehsan, Malaysia
    J Ind Microbiol Biotechnol 38:229-34. 2011
    ..1°C, 272.8 min, 20 mg enzyme amount and 7.8:1 alcohol:acid molar ratio, the esterification percentage was 85.0%. The model can present a rapid means for estimating the conversion yield of succinate ester within the selected ranges...
  21. pmc Crystallization and preliminary X-ray crystallographic analysis of a thermostable organic solvent-tolerant lipase from Bacillus sp. strain 42
    Mohd Saif Khusaini
    Enzyme and Microbial Technology Research, Faculty of Biotechnology and Biomolecular Sciences, University Putra Malaysia, Serdang, Selangor 43400, Malaysia
    Acta Crystallogr Sect F Struct Biol Cryst Commun 67:401-3. 2011
    ..0 Å resolution using an in-house diffractometer. The estimated crystal dimensions were 0.09×0.19×0.08 mm. The crystal belonged to the monoclinic space group C2, with unit-cell parameters a=117.41, b=80.85, c=99.44 Å, β=96.40°...
  22. doi request reprint High yield lipase-catalyzed synthesis of Engkabang fat esters for the cosmetic industry
    Nur Fariza Abd Rahman
    Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, UPM Serdang, Selangor, Malaysia
    Bioresour Technol 102:2168-76. 2011
    ..The actual yields obtained compared well with the predicted values of 100.0% and 91.5%, respectively. Meanwhile, the properties of the esters show that they are suitable to be used as ingredient for cosmetic applications...
  23. doi request reprint Application of artificial neural network for yield prediction of lipase-catalyzed synthesis of dioctyl adipate
    Mohd Basyaruddin Abdul Rahman
    Department of Chemistry, Faculty of Science, 43400 UPM, Serdang, Universiti Putra Malaysia, Serdang, Selangor Darul Ehsan, Malaysia
    Appl Biochem Biotechnol 158:722-35. 2009
    ..In further part of the study, the feedforward backpropagation model was used for prediction of the ester yield within the given range of the main parameters...
  24. ncbi request reprint Production of L2 lipase by Bacillus sp. strain L2: nutritional and physical factors
    Fairolniza Mohd Shariff
    Enzyme and Microbial Technology Research, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang, Selangor, Malaysia
    J Basic Microbiol 47:406-12. 2007
    ..0, 150 rpm of agitation rate and 1% of starting inoculum size. The activity staining of crude L2 lipase revealed a clearing zone at 39 kDa...
  25. doi request reprint Bacteriocin release protein-mediated secretory expression of recombinant chalcone synthase in Escherichia coli
    Iffah Izzati Zakaria
    Faculty of Biotechnology and Biomolecular Sciences, Enzyme and Microbial Technology Research Group, Universiti Putra Malaysia, Serdang, Malaysia
    Appl Biochem Biotechnol 165:737-47. 2011
    ..coli Tuner (DE3) plysS. The optimized conditions, which include the incubation of cells for 20 h with 40 ng/ml mitomycin C at OD(600) induction time of 0.5 was found to be the best condition for chalcone synthase secretion...
  26. ncbi request reprint A novel organic solvent tolerant lipase from Bacillus sphaericus 205y: extracellular expression of a novel OST-lipase gene
    Moohamad Ropaning Sulong
    Enzyme and Microbial Technology Research, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Malaysia
    Protein Expr Purif 49:190-5. 2006
    ..Studies on the effect of oil showed that the lipase was most active in the presence of tricaprin (C10). The lipase exhibited 1,3 positional specificity. Keywords: Bacter..
  27. pmc Phase behaviour and formation of fatty acid esters nanoemulsions containing piroxicam
    Nursyamsyila Mat Hadzir
    Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia
    AAPS PharmSciTech 14:456-63. 2013
    ..The results proved that the prepared nanoemulsions system cannot be formed spontaneously, and thus, energy input was required to produce nano-sized range particles...
  28. ncbi request reprint Preparation of emulsions by rotor-stator homogenizer and ultrasonic cavitation for the cosmeceutical industry
    Ng Sook Han
    Universiti Putra Malaysia, Selangor, Malaysia
    J Cosmet Sci 63:333-44. 2012
    ..A highly stable emulsion was prepared using ultrasonic cavitation comprising a very small particle size with higher zeta potential value and G' > G″ demonstrating gel-like behavior...
  29. ncbi request reprint S5 Lipase: an organic solvent tolerant enzyme
    Raja Noor Zaliha Abdul Rahman
    Enzyme and Microbial Technology Research, University Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia
    J Microbiol 44:583-90. 2006
    ..The absence of magnesium ions (Mg2+) in the basal medium was also shown to stimulate lipase production. Meanwhile, an alkaline earth metal ion, Na+, was found to stimulate the production of S5 lipase...
  30. pmc A modeling study by response surface methodology and artificial neural network on culture parameters optimization for thermostable lipase production from a newly isolated thermophilic Geobacillus sp. strain ARM
    Afshin Ebrahimpour
    Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia
    BMC Biotechnol 8:96. 2008
    ..The most important stages in a biological process are modeling and optimization to improve a system and increase the efficiency of the process without increasing the cost...
  31. pmc Geobacillus zalihae sp. nov., a thermophilic lipolytic bacterium isolated from palm oil mill effluent in Malaysia
    Raja Noor Zaliha Raja Abd Rahman
    Enzyme and Microbial Technology Research Group, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia
    BMC Microbiol 7:77. 2007
    ..Thermophiles harboring intrinsically stable enzymes are suitable for industrial applications. The quest for intrinsically thermostable lipases from thermophiles is a prominent task due to the laborious processes via genetic modification...
  32. doi request reprint Reductive alkylation causes the formation of a molten globule-like intermediate structure in Geobacillus zalihae strain T1 thermostable lipase
    Kok Whye Cheong
    Institute of Bioscience, Universiti Putra Malaysia, Serdang, Selangor, Malaysia
    Appl Biochem Biotechnol 164:362-75. 2011
    ..Based on matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) analysis, a small number of lysine residues were confirmed to be alkylated...
  33. ncbi request reprint Secretory expression of thermostable T1 lipase through bacteriocin release protein
    Raja Noor Zaliha Raja Abdul Rahman
    Faculty of Biotechnology and Biomolecular Sciences, Enzyme and Microbial Technology Research Group, Universiti Putra Malaysia, 43400 UPM Serdang, Malaysia
    Protein Expr Purif 40:411-6. 2005
    ..IPTG at 0.05 mM, YT medium, induction at A(600 nm) of 1.25, 30 degrees C, and 32 h of induction time were best condition for T1 lipase secretion with Origami B as a host...
  34. pmc Crystallization and preliminary X-ray crystallographic analysis of highly thermostable L2 lipase from the newly isolated Bacillus sp. L2
    Fairolniza Mohd Shariff
    Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia
    Acta Crystallogr Sect F Struct Biol Cryst Commun 66:715-7. 2010
    ..44, b = 94.90, c = 126.46 A. The asymmetric unit contained one single molecule of protein, with a Matthews coefficient (V(M)) of 2.85 A(3) Da(-1) and a solvent content of 57%...
  35. ncbi request reprint On the importance of the small domain in the thermostability of thermoalkalophilic lipases from l1 and t1: insights from molecular dynamics simulation
    Roghayeh Abedi Karjiban
    Department of Chemistry, Faculty of Science, Universiti Putra Malaysia
    Protein Pept Lett 17:699-707. 2010
    ..The results appear to propose that these regions are critical in determining of the overall thermal stability of both organisms...
  36. ncbi request reprint Optimization of enzymatic synthesis of palm-based kojic acid ester using response surface methodology
    Siti Efliza Ashari
    Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang, Selangor, Malaysia
    J Oleo Sci 58:503-10. 2009
    ..21%. The results demonstrated that response surface methodology can be applied effectively to optimize the lipase-catalysed synthesis of kojic acid monooleate. The optimum conditions can be used to scale up the process...
  37. ncbi request reprint High-yield purification of an organic solvent-tolerant lipase from Pseudomonas sp. strain S5
    Raja Noor Zaliha R A Rahman
    Enzyme and Microbial Technology Research, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia
    Anal Biochem 341:267-74. 2005
    ..The S5 lipase exhibited the highest activity in the presence of palm oil as a natural oil and triolein as a synthetic triglyceride. It showed random positional specificity on the thin-layer chromatography...
  38. pmc Solution structures, dynamics, and ice growth inhibitory activity of peptide fragments derived from an antarctic yeast protein
    Syed Hussinien H Shah
    Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, UPM Serdang, Selangor, Malaysia
    PLoS ONE 7:e49788. 2012
    ..This report represents the first detailed report of downsizing a yeast AFP into its peptide fragments with measurable antifreeze activities...
  39. pmc Combination of Oxyanion Gln114 Mutation and Medium Engineering to Influence the Enantioselectivity of Thermophilic Lipase from Geobacillus zalihae
    Roswanira Abdul Wahab
    Enzyme and Microbial Technology Group, Faculty of Science, Universiti Putra Malaysia, Serdang, Selangor 43400, Malaysia E mail Faculty of Science, Universiti Teknologi Malaysia, Skudai, Johor 81310, Malaysia
    Int J Mol Sci 13:11666-80. 2012
    ..The higher desiccant loading also increased viscosity in the reaction and further reduced the efficiency of the lipase-catalyzed esterifications...
  40. doi request reprint Comparison of the estimation capabilities of response surface methodology and artificial neural network for the optimization of recombinant lipase production by E. coli BL21
    Rubina Nelofer
    Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, University Putra Malaysia, 43400 Serdang, Selangor, Malaysia
    J Ind Microbiol Biotechnol 39:243-54. 2012
    ..2 IU/mL) and the predicted lipase activity was also closer to the observed data at these levels, suggesting that ANN is a better optimization method than RSM for lipase production by the recombinant strain...
  41. ncbi request reprint Characterization and effect on skin hydration of engkabang-based emulsions
    Siti Salwa Abd Gani
    Faculty of Science, Universiti Putra Malaysia, Selangor, Malaysia
    Biosci Biotechnol Biochem 74:1188-93. 2010
    ..35 x 10(-12) and 2.77 x 10(-15) for F10 and E15 respectively. The hydration of the skins increased after application of F10 and E15 with p-value below 0.05...
  42. ncbi request reprint A thermoalkaliphilic lipase of Geobacillus sp. T1
    Thean Chor Leow
    Enzyme and Microbial Technology Research Group, Universiti Putra Malaysia, 43400, UPM Serdang, Selangor, Malaysia
    Extremophiles 11:527-35. 2007
    ..Serine and aspartate residues were involved in catalysis, as its activity was strongly inhibited by 5 mM PMSF and 1 mM Pepstatin. The T(m) for T1 lipase was around 72.2 degrees C, as revealed by denatured protein analysis of CD spectra...
  43. doi request reprint Molecular dynamics study of the structure, flexibility and dynamics of thermostable l1 lipase at high temperatures
    Roghayeh Abedi Karjiban
    Department of Chemistry, Universiti Putra Malaysia, UPM Serdang, 43400, Selangor, Malaysia
    Protein J 28:14-23. 2009
    ..The results appear to suggest that tight interactions of Zn(2+)-binding coordination with specified residues became weak at high temperature which suggests the contribution of this region to the thermostability of the enzyme...
  44. doi request reprint Cold-adapted RTX lipase from antarctic Pseudomonas sp. strain AMS8: isolation, molecular modeling and heterologous expression
    Mohd Shukuri Mohamad Ali
    Enzyme and Microbial Technology Research Center, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang, Selangor 43400, Malaysia
    Protein J 32:317-25. 2013
    ..The optimal expression level was attained using the recombinant plasmid pET32b/BL21(DE3) expressed at 15 °C for 8 h, induced by 0.1 mM isopropyl β-D thiogalactoside (IPTG) at E. coli growth optimal density of 0.5...
  45. pmc Structural adaptation of cold-active RTX lipase from Pseudomonas sp. strain AMS8 revealed via homology and molecular dynamics simulation approaches
    Mohd Shukuri Mohamad Ali
    Enzyme and Microbial Technology Research Center, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia
    Biomed Res Int 2013:925373. 2013
    ..The information obtained could be a useful tool for low temperature industrial applications and molecular engineering purposes, in the near future...
  46. pmc Capillary-Seeding Crystallization and Preliminary Crystallographic Analysis of a Solvent-Tolerant Elastase from Pseudomonas aeruginosa Strain K
    Mohd Shukuri Mohamad Ali
    Enzyme and Microbial Technology Research Centre, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang UPM 43400, Selangor, Malaysia
    Int J Mol Sci 14:17608-17. 2013
    ..39 Å at SPring-8, Japan, using synchrotron radiation for preliminary data diffraction analysis. The space group was determined to be monoclinic space group P1211 with unit cell parameters of a = 38.99 Ǻ, b = 90.173 Å and c = 40.60 Å. ..
  47. ncbi request reprint Effect of ion pair on thermostability of F1 protease: integration of computational and experimental approaches
    Raja Noor Zaliha Raja Abd Rahman
    Enzyme and Microbial Technology Research Group, Selangor, Malaysia
    J Microbiol Biotechnol 22:34-45. 2012
    ..The effects of addition and disruption of ion pair networks on the activity and various stabilities of mutant F1 proteases were compared with those of the wild-type F1 protease...
  48. pmc Improvement of Thermal Stability via Outer-Loop Ion Pair Interaction of Mutated T1 Lipase from Geobacillus zalihae Strain T1
    Rudzanna Ruslan
    Enzyme and Microbial Technology Laboratory, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia E Mails R R T C L M S M A A B S
    Int J Mol Sci 13:943-60. 2012
    ..The additional ion pair in D311E may regulate the stability of this mutant lipase at high temperatures as predicted in silico and spectroscopically...
  49. doi request reprint Multivariate optimization in the biosynthesis of a triethanolamine (TEA)-based esterquat cationic surfactant using an artificial neural network
    Hamid Reza Fard Masoumi
    Department of Chemistry, Faculty of Science, University Putra Malaysia, 43400 Selangor, Malaysia
    Molecules 16:5538-49. 2011
    ..This method represents a more flexible and convenient means for optimizing enzymatic reaction using ANN than has been previously reported by conventional methods...
  50. ncbi request reprint Physical factors affecting the production of organic solvent-tolerant protease by Pseudomonas aeruginosa strain K
    Raja Noor Zaliha Abd Rahman
    Department of Biochemistry and Microbiology, Enzyme and Microbial Technology Research, Faculty of Science and Environmental Studies, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia
    Bioresour Technol 96:429-36. 2005
    ..0 to 9.0. However, the highest yield of protease was observed at pH 7.0. Neutral media increased the protease production compared to acidic or alkaline media...
  51. doi request reprint Determining optimum conditions for lipase-catalyzed synthesis of triethanolamine (TEA)-based esterquat cationic surfactant by a Taguchi robust design method
    Hamid Reza Fard Masoumi
    Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, 43400 Selangor, Malaysia
    Molecules 16:4672-80. 2011
    ..The amount of enzyme in the reaction had also a significant effect on reaction conversion%...
  52. ncbi request reprint Anticancer activity of 3-O-acylated betulinic acid derivatives obtained by enzymatic synthesis
    Faujan Bin H Ahmad
    Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia
    Biosci Biotechnol Biochem 74:1025-9. 2010
    ..In an ovarian cancer cell line, all betulinic acid derivatives prepared showed weaker cytotoxicity than betulinic acid...
  53. ncbi request reprint Biodegradation of hydrocarbon contamination by immobilized bacterial cells
    Raja Noor Zaliha Abd Rahman
    Enzyme and Microbial Technology Research, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra, Malaysia
    J Microbiol 44:354-9. 2006
    ..We conclude that immobilized hydrocarbon-degrading bacteria represent a promising application in the bioremediation of hydrocarbon-contaminated areas...
  54. ncbi request reprint Reductive alkylation of lipase: experimental and molecular modeling approaches
    Raja Noor Zaliha A Rahman
    Enzyme and Microbial Technology Research, Faculty of Science and Environmental Studies, Institute of Bioscience, Universiti Putra Malaysia, 43400 Serdang
    Appl Biochem Biotechnol 118:11-20. 2004
    ....
  55. doi request reprint Green nanoemulsion-laden glyphosate isopropylamine formulation in suppressing creeping foxglove (A. gangetica), slender button weed (D. ocimifolia) and buffalo grass (P. conjugatum)
    Chaw Jiang Lim
    Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, Selangor, Malaysia
    Pest Manag Sci 69:104-11. 2013
    ....
  56. ncbi request reprint Secretory expression of thermostable alkaline protease from Bacillus stearothermophilus FI by using native signal peptide and α-factor secretion signal in Pichia pastoris
    Amaliawati Ahmad Latiffi
    Enzyme and Microbial Technology Research Center, 43400 Serdang, Selangor, Malaysia
    Genes Genet Syst 88:85-91. 2013
    ..Production medium YPTD was found to be the best for F1 protease expression with the highest yield of 4.13 U/mL. The protein was expressed as His-tagged fusion protein with a size about 34 kDa. ..
  57. pmc Modification of palm kernel oil esters nanoemulsions with hydrocolloid gum for enhanced topical delivery of ibuprofen
    Norazlinaliza Salim
    Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, Selangor, Malaysia
    Int J Nanomedicine 7:4739-47. 2012
    ..The present study investigated the modification of nanoemulsions with different hydrocolloid gums, to enhanced drug delivery of ibuprofen. The in vitro characterization of the initial and modified nanoemulsions was also studied...
  58. pmc Enzymatic Properties and Mutational Studies of Chalcone Synthase from Physcomitrella patens
    Raja Noor Zaliha Raja Abdul Rahman
    Faculty of Biotechnology and Biomolecular Sciences, Enzyme and Microbial Technology Research Group, Universiti Putra Malaysia, Selangor 43400, Malaysia E Mails I I Z A B S
    Int J Mol Sci 13:9673-91. 2012
    ..These substitutions are believed to have a restricted number of elongations of the growing polypeptide chain due to the smaller cavity volume of the mutant's active site...
  59. pmc 3D Structure Elucidation of Thermostable L2 Lipase from Thermophilic Bacillus sp. L2
    Raja Noor Zaliha Raja Abd Rahman
    Faculty of Biotechnology and Biomolecular Sciences, Enzyme and Microbial Technology Research, Universiti Putra Malaysia, UPM Serdang, Selangor 43400, Malaysia E Mails F M S A B S
    Int J Mol Sci 13:9207-17. 2012
    ..Ser-113, His-358 and Asp-317 were assigned as catalytic triad residues. One Ca(2+) and one Zn(2+) were found in the L2 lipase molecule...
  60. ncbi request reprint Immobilization of lipase from Candida rugosa on layered double hydroxides for esterification reaction
    Mohd Basyaruddin A Rahman
    Centre for Research in Enzyme and Microbial Technology, Faculty of Science and Environmental Studies, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor
    Appl Biochem Biotechnol 118:313-20. 2004
    ..Stability was found to be the highest in the nanocomposite NAZAD...
  61. pmc Role of α-Helical Structure in Organic Solvent-Activated Homodimer of Elastase Strain K
    Raja Noor Zaliha Raja Abd Rahman
    Enzyme and Microbial Technology Laboratory, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, UPM Serdang 43400, Selangor, Malaysia E Mails A B S C F W
    Int J Mol Sci 12:5797-814. 2011
    ....
  62. doi request reprint An improved method for the preparations of nanostructured lipid carriers containing heat-sensitive bioactives
    Loo Chew Hung
    Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, UPM, Serdang, Selangor, Malaysia
    Colloids Surf B Biointerfaces 87:180-6. 2011
    ..The physical stability of the formulation was maintained throughout study duration...
  63. pmc A Newly Isolated Thermostable Lipase from Bacillus sp
    Fairolniza Mohd Shariff
    Enzyme and Microbial Technology Research, Faculty of Biotechnology and Biomolecular Sciences, University Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia E Mails F M S A B S
    Int J Mol Sci 12:2917-34. 2011
    ..The CD spectra of secondary structure analysis showed that the L2 lipase structure contained 38.6% α-helices, 2.2% ß-strands, 23.6% turns and 35.6% random conformations...
  64. pmc Optimization of lipase-mediated synthesis of 1-nonene oxide using phenylacetic Acid and hydrogen peroxide
    Emilia Abdulmalek
    Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia
    Int J Mol Sci 13:13140-9. 2012
    ..Interestingly, the enzyme was stable under the single-step addition of H(2)O(2) with a catalytic activity of 190.0 Ug-1. The entire epoxidation process was carried out within 12 h using a conventional water bath shaker...
  65. pmc Effect of alcohol structure on the optimum condition for novozym 435-catalyzed synthesis of adipate esters
    Mohd Basyaruddin Abdul Rahman
    Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, 43400 Serdang, Malaysia
    Biotechnol Res Int 2011:162987. 2011
    ..1%. The enzyme is highly efficient biocatalyst for the synthesis of adipate esters by offering a simple production process and a high esterification yield...
  66. ncbi request reprint High level expression of thermostable lipase from Geobacillus sp. strain T1
    Thean Chor Leow
    Enzyme and Microbial Technology Research Group, Faculty of Science and Environmental Studies, Universiti Putra Malaysia, Serdang, Malaysia
    Biosci Biotechnol Biochem 68:96-103. 2004
    ..It was stable up to 65 degrees C at pH 7 and active over a wide pH range (pH 6-11). This study presents a rapid cloning and overexpression, aimed at improving the enzyme yield for successful industrial application...
  67. doi request reprint Synthesis and physico-chemical properties of new tetraethylammonium-based amino acid chiral ionic liquids
    Mohd Basyaruddin Abdul Rahman
    Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, UPM Serdang, Selangor, Malaysia
    Molecules 15:2388-97. 2010
    ..Several new CILs prepared in this work showed high ionic conductivity compared to the amino acid ionic liquids (AAILs) found in the literature...
  68. pmc Expression of an Organic Solvent Stable Lipase from Staphylococcus epidermidis AT2
    Raja Noor Zaliha Raja Abd Rahman
    Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang, Selangor, Malaysia E Mail N H A K
    Int J Mol Sci 11:3195-208. 2010
    ..and Bacillus sp., but very few from Staphylococcus sp. This lipase demonstrates great potential to be employed in various industrial applications...
  69. pmc Formulation optimization of a palm-based nanoemulsion system containing levodopa
    Syafinaz Zainol
    Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia
    Int J Mol Sci 13:13049-64. 2012
    ..The experimental data could be fitted sufficiently well into a second-order polynomial model. The optimized formulation was stable for six months at 4 °C...
  70. pmc Optimization of physical factors affecting the production of thermo-stable organic solvent-tolerant protease from a newly isolated halo tolerant Bacillus subtilis strain Rand
    Randa A Abusham
    Enzyme and Microbial Technology Research Group, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia
    Microb Cell Fact 8:20. 2009
    ..abstract:..
  71. ncbi request reprint Secretory expression in Escherichia coli and single-step purification of a heat-stable alkaline protease
    Zhibiao Fu
    Department of Biochemistry, Hongkong University of Science and Technology, 43400 UPM Serdang, Selangor, Malaysia
    Protein Expr Purif 28:63-8. 2003
    ..0 to 10.0. The enzyme exhibited a high degree of thermostability with a half-life of 4 h at 85 degrees C, 25 min at 90 degrees C, and was inhibited by the serine protease inhibitor phenylmethylsulfonyl fluoride (PMSF)...
  72. ncbi request reprint Novel cation-pi interaction revealed by crystal structure of thermoalkalophilic lipase
    Hiroyoshi Matsumura
    Department of Applied Chemistry, Graduate School of Engineering, Osaka University, 2 1, Yamadaoka, Suita, Osaka 565 0871, Japan
    Proteins 70:592-8. 2008