Sheila Nathan

Summary

Country: Malaysia

Publications

  1. pmc Complete killing of Caenorhabditis elegans by Burkholderia pseudomallei is dependent on prolonged direct association with the viable pathogen
    Song Hua Lee
    School of Biosciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, UKM Bangi, Selangor, Malaysia
    PLoS ONE 6:e16707. 2011
  2. pmc Immunogenic recombinant Burkholderia pseudomallei MprA serine protease elicits protective immunity in mice
    Chui Yoke Chin
    Faculty of Science and Technology, School of Biosciences and Biotechnology, Universiti Kebangsaan Malaysia, Bangi Selangor DE, Malaysia
    Front Cell Infect Microbiol 2:85. 2012
  3. pmc Immunogenic Eimeria tenella glycosylphosphatidylinositol-anchored surface antigens (SAGs) induce inflammatory responses in avian macrophages
    Yock Ping Chow
    School of Biosciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, Bangi, Selangor D E, Malaysia
    PLoS ONE 6:e25233. 2011
  4. pmc Discovery of potential anti-infectives against Staphylococcus aureus using a Caenorhabditis elegans infection model
    Cin Kong
    School of Biosciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor, Malaysia
    BMC Complement Altern Med 14:4. 2014
  5. pmc Multiple-antigen ELISA for melioidosis--a novel approach to the improved serodiagnosis of melioidosis
    Yuka Hara
    Malaysia Genome Institute, Jalan Bangi, Kajang, Selangor D E 43000, Malaysia
    BMC Infect Dis 13:165. 2013
  6. pmc InCoB celebrates its tenth anniversary as first joint conference with ISCB-Asia
    Christian Schonbach
    Department of Bioscience and Bioinformatics, Graduate School of Computer Science and Systems Engineering, Kyushu Institute of Technology, Fukuoka 820 8502, Japan
    BMC Genomics 12:S1. 2011
  7. pmc Towards big data science in the decade ahead from ten years of InCoB and the 1st ISCB-Asia Joint Conference
    Shoba Ranganathan
    Department of Chemistry and Biomolecular Sciences and ARC Centre of Excellence in Bioinformatics, Macquarie University, Sydney NSW 2109, Australia
    BMC Bioinformatics 12:S1. 2011
  8. ncbi request reprint Immunization with the recombinant Burkholderia pseudomallei outer membrane protein Omp85 induces protective immunity in mice
    Yu Ching Su
    School of Biosciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor Darul Ehsan, Malaysia
    Vaccine 28:5005-11. 2010
  9. doi request reprint Catalysis by Glomerella cingulata cutinase requires conformational cycling between the active and inactive states of its catalytic triad
    Mun Peak Nyon
    School of Biosciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, Selangor, Malaysia
    J Mol Biol 385:226-35. 2009
  10. doi request reprint A genome level survey of Burkholderia pseudomallei immunome expressed during human infection
    Yu Ching Su
    School of Biosciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor Darul Ehsan, Malaysia
    Microbes Infect 10:1335-45. 2008

Collaborators

Detail Information

Publications34

  1. pmc Complete killing of Caenorhabditis elegans by Burkholderia pseudomallei is dependent on prolonged direct association with the viable pathogen
    Song Hua Lee
    School of Biosciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, UKM Bangi, Selangor, Malaysia
    PLoS ONE 6:e16707. 2011
    ..Much remains to be known about the contributions of genotypic variations within the bacteria and the host, and environmental factors that lead to the manifestation of the clinical symptoms of melioidosis...
  2. pmc Immunogenic recombinant Burkholderia pseudomallei MprA serine protease elicits protective immunity in mice
    Chui Yoke Chin
    Faculty of Science and Technology, School of Biosciences and Biotechnology, Universiti Kebangsaan Malaysia, Bangi Selangor DE, Malaysia
    Front Cell Infect Microbiol 2:85. 2012
    ..In conclusion, MprA is immunogenic in melioidosis patients whilst also eliciting a strong immune response upon bacterial challenge in mice and presents itself as a potential vaccine candidate for the treatment of melioidosis...
  3. pmc Immunogenic Eimeria tenella glycosylphosphatidylinositol-anchored surface antigens (SAGs) induce inflammatory responses in avian macrophages
    Yock Ping Chow
    School of Biosciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, Bangi, Selangor D E, Malaysia
    PLoS ONE 6:e25233. 2011
    ....
  4. pmc Discovery of potential anti-infectives against Staphylococcus aureus using a Caenorhabditis elegans infection model
    Cin Kong
    School of Biosciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor, Malaysia
    BMC Complement Altern Med 14:4. 2014
    ....
  5. pmc Multiple-antigen ELISA for melioidosis--a novel approach to the improved serodiagnosis of melioidosis
    Yuka Hara
    Malaysia Genome Institute, Jalan Bangi, Kajang, Selangor D E 43000, Malaysia
    BMC Infect Dis 13:165. 2013
    ..Hence, rapid diagnosis of melioidosis is crucial for the successful management of melioidosis...
  6. pmc InCoB celebrates its tenth anniversary as first joint conference with ISCB-Asia
    Christian Schonbach
    Department of Bioscience and Bioinformatics, Graduate School of Computer Science and Systems Engineering, Kyushu Institute of Technology, Fukuoka 820 8502, Japan
    BMC Genomics 12:S1. 2011
    ..1%) were accepted. The strong showing of Asia among submissions (82.7%) and acceptances (81.6%) signals the success of this tenth InCoB anniversary meeting, and bodes well for the future of ISCB-Asia...
  7. pmc Towards big data science in the decade ahead from ten years of InCoB and the 1st ISCB-Asia Joint Conference
    Shoba Ranganathan
    Department of Chemistry and Biomolecular Sciences and ARC Centre of Excellence in Bioinformatics, Macquarie University, Sydney NSW 2109, Australia
    BMC Bioinformatics 12:S1. 2011
    ....
  8. ncbi request reprint Immunization with the recombinant Burkholderia pseudomallei outer membrane protein Omp85 induces protective immunity in mice
    Yu Ching Su
    School of Biosciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor Darul Ehsan, Malaysia
    Vaccine 28:5005-11. 2010
    ..pseudomallei by human polymorphonuclear cells. In conclusion, we demonstrated that B. pseudomallei Omp85 is potentially able to induce protective immunity against melioidosis...
  9. doi request reprint Catalysis by Glomerella cingulata cutinase requires conformational cycling between the active and inactive states of its catalytic triad
    Mun Peak Nyon
    School of Biosciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, Selangor, Malaysia
    J Mol Biol 385:226-35. 2009
    ....
  10. doi request reprint A genome level survey of Burkholderia pseudomallei immunome expressed during human infection
    Yu Ching Su
    School of Biosciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor Darul Ehsan, Malaysia
    Microbes Infect 10:1335-45. 2008
    ..These immunogens can be further evaluated as prophylactic and serodiagnostic candidates as well as drug targets...
  11. pmc Immunogenic Burkholderia pseudomallei outer membrane proteins as potential candidate vaccine targets
    Yuka Hara
    Malaysia Genome Institute, UKM MTDC Smart Technology Centre, Bangi, Selangor, Malaysia
    PLoS ONE 4:e6496. 2009
    ..There is no vaccine towards the bacterium available in the market, and the efficacy of many of the bacterium's surface and secreted proteins are currently being evaluated as vaccine candidates...
  12. ncbi request reprint Burkholderia pseudomallei animal and human isolates from Malaysia exhibit different phenotypic characteristics
    Song Hua Lee
    Faculty of Science and Technology, School of Biosciences and Biotechnology, Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor Darul Ehsan, Malaysia
    Diagn Microbiol Infect Dis 58:263-70. 2007
    ..pseudomallei-susceptible BALB/c mice. Most of the clinical isolates were highly virulent. However, virulence did not correlate with isolate origin since 2 of the animal isolates were also highly virulent...
  13. ncbi request reprint Genome-wide prediction and annotation of Burkholderia pseudomallei AraC/XylS family transcription regulator
    Boon San Lim
    School of Biosciences and Biotechnology, Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor, Malaysia
    In Silico Biol 7:389-97. 2007
    ..This paper has demonstrated an efficient and systematic genome-wide scale prediction of the AraC/XylS family that can be applied to other protein families...
  14. pmc Burkholderia pseudomallei suppresses Caenorhabditis elegans immunity by specific degradation of a GATA transcription factor
    Song Hua Lee
    School of Biosciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor, Malaysia
    Proc Natl Acad Sci U S A 110:15067-72. 2013
    ..pseudomallei type III secretion system. Together, our studies using an intact host provide evidence for pathogen-mediated host immune suppression through the destruction of a host transcription factor. ..
  15. pmc Burkholderia pseudomallei kills Caenorhabditis elegans through virulence mechanisms distinct from intestinal lumen colonization
    Soon Keat Ooi
    School of Biosciences and Biotechnology, Universiti Kebangsaan Malaysia, Bangi, Selangor, Malaysia
    Virulence 3:485-96. 2012
    ..Collectively, our findings propose that B. pseudomallei specifically and continuously secretes toxins to overcome C. elegans immune responses...
  16. pmc Delayed activation of host innate immune pathways in streptozotocin-induced diabetic hosts leads to more severe disease during infection with Burkholderia pseudomallei
    Chui Yoke Chin
    School of Biosciences and Biotechnology, Universiti Kebangsaan Malaysia, Selangor, Malaysia
    Immunology 135:312-32. 2012
    ..pseudomallei acute infection, poor glycaemic control impaired innate responses during the early stages of B. pseudomallei infection, contributing to the increased susceptibility of STZ-induced diabetics to this fatal disease...
  17. pmc Mammalian sperm fertility related proteins
    Ali Ashrafzadeh
    1 School of Biosciences and Biotechnology, Faculty of Science and Technology, University Kebangsaan Malaysia, Selangor, Malaysia
    Int J Med Sci 10:1649-57. 2013
    ..The data from these studies show the impact of sperm proteome studies on identifying different valuable markers for fertility screening. In this article, we review recent development in unraveling sperm fertility related proteins. ..
  18. doi request reprint Thermal stability engineering of Glomerella cingulata cutinase
    Iuan Sheau Chin
    School of Biosciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor, Malaysia
    Protein Eng Des Sel 26:369-75. 2013
    ..Taken together, our study may provide valuable information for enhancing catalytic performance and thermal stability in future engineering endeavors...
  19. pmc Genome wide transcriptome profiling of a murine acute melioidosis model reveals new insights into how Burkholderia pseudomallei overcomes host innate immunity
    Chui Yoke Chin
    School of Biosciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi Selangor D E Malaysia
    BMC Genomics 11:672. 2010
    ..We compared the transcriptome of infected liver and spleen with uninfected tissues over an infection period of 42 hr to identify genes whose expression is altered in response to an acute infection...
  20. pmc Burkholderia pseudomallei transcriptional adaptation in macrophages
    Sylvia Chieng
    School of Biosciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, Bangi, 43600, Malaysia
    BMC Genomics 13:328. 2012
    ..How the bacterium interacts with host macrophage cells is still not well understood and is critical to appreciate the strategies used by this bacterium to survive and how intracellular survival leads to disease manifestation...
  21. pmc Crystallization and preliminary X-ray analysis of recombinant Glomerella cingulata cutinase
    Mun Peak Nyon
    School of Biosciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 Bangi, Malaysia
    Acta Crystallogr Sect F Struct Biol Cryst Commun 64:504-8. 2008
    ..5 A resolution. All of the crystals are suitable for structural studies, which are currently ongoing...
  22. ncbi request reprint Expression profile of immune-related genes in Lates calcarifer infected by Cryptocaryon irritans
    Norashikin Mohd-Shaharuddin
    School of Biosciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, Selangor, Malaysia
    Fish Shellfish Immunol 34:762-9. 2013
    ..irritans and L. calcarifer and suggest that the innate immune system plays an important role in early defence against parasite infection allowing the fish to eventually recover from the infection...
  23. ncbi request reprint Expression and purification of a recombinant scFv towards the exotoxin of the pathogen, Burkholderia pseudomallei
    Kue Peng Lim
    Centre for Gene Analysis and Technology, School of Biosciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor Darul Ehsan, Malaysia
    J Microbiol 42:126-32. 2004
    ..pseudomallei antigens. Thus, these antibodies should serve as suitable reagents for future affinity purification of the exotoxin...
  24. ncbi request reprint Epitope mapping of Burkholderia pseudomallei serine metalloprotease: identification of serine protease epitope mimics
    Shzu wei Chan
    Centre for Gene Analysis and Technology, School of Biosciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor D E, Malaysia
    FEMS Immunol Med Microbiol 43:37-44. 2005
    ..In addition, these peptide-bearing phages showed competitive inhibition of B. pseudomallei serine metalloprotease binding to the polyclonal IgG...
  25. pmc Computational discovery and RT-PCR validation of novel Burkholderia conserved and Burkholderia pseudomallei unique sRNAs
    Jia Shiun Khoo
    School of Biosciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Malaysia
    BMC Genomics 13:S13. 2012
    ....
  26. ncbi request reprint Neutralization of Burkholderia pseudomallei protease by Fabs generated through phage display
    Sheila Nathan
    School of Biosciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, Selanger
    Biosci Biotechnol Biochem 69:2302-11. 2005
    ..Thus these recombinant antibodies are candidates for immunotherapy and tools to aid in further elucidation of the mechanism of action of the B. pseudomallei protease...
  27. ncbi request reprint Phage displayed Bacillus thuringiensis Cry1Ba4 toxin is toxic to Plutella xylostella
    Sheila Nathan
    School of Biosciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, Selangor, Malaysia
    Curr Microbiol 53:412-5. 2006
    ..Thus, we have successfully displayed the Cry1Ba4 activated toxin on the surface of a phage and demonstrated toxicity towards larvae...
  28. doi request reprint Characterization of Afp1, an antifreeze protein from the psychrophilic yeast Glaciozyma antarctica PI12
    Noor Haza Fazlin Hashim
    School of Biosciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600, Bangi, Selangor, Malaysia
    Extremophiles 17:63-73. 2013
    ..Activity assays of the recombinant Afp1 confirmed the antifreeze protein properties with a high TH value of 0.08 °C...
  29. pmc Transcriptome analysis of the Cryptocaryon irritans tomont stage identifies potential genes for the detection and control of cryptocaryonosis
    Yogeswaran Lokanathan
    School of Biosciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, Selangor, Malaysia
    BMC Genomics 11:76. 2010
    ..irritans genome is virtually unexplored; therefore, we generated and analyzed expressed sequence tags (ESTs) of the parasite to identify genes that encode for surface proteins, excretory/secretory proteins and repeat-containing proteins...
  30. ncbi request reprint Neutralizing chimeric mouse-human antibodies against Burkholderia pseudomallei protease: expression, purification and characterization
    Shzu wei Chan
    Centre for Gene Analysis and Technology, School of Biosciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor D E, Malaysia
    J Biochem Mol Biol 37:556-64. 2004
    ..These were isolated from high titer serum by affinity chromatography on recombinant-Protein A-Sepharose. Purified polyclonal antibody specificity towards B. pseudomallei protease was proven by Western blotting and ELISA...
  31. pmc Comparative analysis of Mafriwal (Bos taurus × Bos indicus) and Kedah Kelantan (Bos indicus) sperm proteome identifies sperm proteins potentially responsible for higher fertility in a tropical climate
    Ali Ashrafzadeh
    School of Biosciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, Bangi UKM 43600, Selangor, Malaysia
    Int J Mol Sci 14:15860-77. 2013
    ....
  32. ncbi request reprint Detection of virulent Newcastle disease virus using a phage-capturing dot blot assay
    Thong Chuan Lee
    Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, University Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia
    J Virol Methods 136:224-9. 2006
    ..This phage clone was employed as a diagnostic reagent in a dot blot assay and it successfully detected only virulent NDV strains...
  33. ncbi request reprint In silico analysis of Burkholderia pseudomallei genome sequence for potential drug targets
    Chan Eng Chong
    School of Biosciences and Biotechnology, Faculty of Science and Technology, University Kebangsaan Malaysia, 43600 Bangi, Malaysia
    In Silico Biol 6:341-6. 2006
    ..Therefore, our in silico approach has enabled rapid screening and identification of potential drug targets for further characterization in the laboratory...
  34. ncbi request reprint Bacterial expression of the scFv fragment of a recombinant antibody specific for Burkholderia pseudomallei exotoxin
    Yu Ching Su
    Centre for Gene Analysis and Technology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor D E, Malaysia
    J Biochem Mol Biol 36:493-8. 2003
    ..05%. However, by indirect ELISA, only the scFv protein that was expressed in 0.2% (v/v) glycerol exhibited high specificity towards the Burkholderia pseudomallei exotoxin...