Levi Makala

Summary

Affiliation: GHSU
Location: Augusta, USA

Publications

  1. pmc The role of indoleamine 2, 3 dioxygenase in regulating host immunity to leishmania infection
    Levi H C Makala
    Georgia Health Sciences University, Medical College of Georgia, Department of Pediatrics, Hematology Oncology Section, Augusta, Georgia 30912, USA
    J Biomed Sci 19:5. 2012
  2. ncbi Neospora caninum NcSRS2 is a transmembrane protein that contains a glycosylphosphatidylinositol anchor in insect cells
    Yoshifumi Nishikawa
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada cho, Obihiro, Hokkaido 080 8555, Japan
    Vet Parasitol 109:191-201. 2002
  3. ncbi Peyer's patches: organized lymphoid structures for the induction of mucosal immune responses in the intestine
    L H C Makala
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan
    Pathobiology 70:55-68. 2002
  4. ncbi Seasonal change in the number of Cryptosporidium parvum oocysts in water samples from the rivers in Hokkaido, Japan, detected by the ferric sulfate flocculation method
    Yoshinori Tsushima
    National Research Center for Protozoan Diseases, Obihiro University, Hokkaido, Japan
    J Vet Med Sci 65:121-3. 2003
  5. ncbi The current status of major tick borne diseases in Zambia
    Levi Hakwale Makala
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada cho, Obihiro, Hokkaido 080 8555, Japan
    Vet Res 34:27-45. 2003
  6. ncbi Unresponsiveness to surface antigen 1 modifies cytokine profiles in acute Toxoplasma gondii infection
    S Seng
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan
    Pathobiology 70:237-46. 2002
  7. ncbi Phenotype and function of murine discrete Peyer's patch macrophage derived - dendritic cells
    Levi H C Makala
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada cho, Hokkaido, Japan
    J Vet Med Sci 65:491-9. 2003
  8. ncbi Viability and infectivity of Cryptosporidium parvum oocysts detected in river water in Hokkaido, Japan
    Yoshinori Tsushima
    National Research Center for Protozoan Diseases, Obihiro University, Hokkaido, Japan
    J Vet Med Sci 65:585-9. 2003
  9. ncbi A comparison of the phenotype of dendritic cells derived from discrete Peyer's patch macrophages of non-infected and Toxoplasma gondii infected mice
    Levi H C Makala
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan
    J Vet Med Sci 65:591-7. 2003
  10. ncbi A role for balance of interferon-gamma and interleukin-4 production in protective immunity against Neospora caninum infection
    Yoshifumi Nishikawa
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada cho, Obihiro, Hokkaido 080 8555, Japan
    Vet Parasitol 116:175-84. 2003

Collaborators

Detail Information

Publications33

  1. pmc The role of indoleamine 2, 3 dioxygenase in regulating host immunity to leishmania infection
    Levi H C Makala
    Georgia Health Sciences University, Medical College of Georgia, Department of Pediatrics, Hematology Oncology Section, Augusta, Georgia 30912, USA
    J Biomed Sci 19:5. 2012
    ....
  2. ncbi Neospora caninum NcSRS2 is a transmembrane protein that contains a glycosylphosphatidylinositol anchor in insect cells
    Yoshifumi Nishikawa
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada cho, Obihiro, Hokkaido 080 8555, Japan
    Vet Parasitol 109:191-201. 2002
    ..We have likewise shown the usefulness of the insect-recombinant baculovirus system in the expression of rNcSRS2...
  3. ncbi Peyer's patches: organized lymphoid structures for the induction of mucosal immune responses in the intestine
    L H C Makala
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan
    Pathobiology 70:55-68. 2002
    ..The present understanding of PP biology and function, taking into account their preferential and unique retention of immune competent cells at specific sites, is discussed...
  4. ncbi Seasonal change in the number of Cryptosporidium parvum oocysts in water samples from the rivers in Hokkaido, Japan, detected by the ferric sulfate flocculation method
    Yoshinori Tsushima
    National Research Center for Protozoan Diseases, Obihiro University, Hokkaido, Japan
    J Vet Med Sci 65:121-3. 2003
    ..50, 5.00 and 3.33 oocysts/l, respectively. The oocyst density in river water changed in relation to the season in 1999, 2000 and 2001. This report first cleared up the seasonal changes in C. parvum oocysts number in river water...
  5. ncbi The current status of major tick borne diseases in Zambia
    Levi Hakwale Makala
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada cho, Obihiro, Hokkaido 080 8555, Japan
    Vet Res 34:27-45. 2003
    ..It is thus hoped that this review will stimulate the desire to maximize the value of the tick and tick-borne disease research in both Zambia and the international research community...
  6. ncbi Unresponsiveness to surface antigen 1 modifies cytokine profiles in acute Toxoplasma gondii infection
    S Seng
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan
    Pathobiology 70:237-46. 2002
    ..gondii infection. These results provide evidence that SAG1 is a pivotal antigen involved in the induction of immune responses towards the development of Th1-protective immunity during T. gondii infection...
  7. ncbi Phenotype and function of murine discrete Peyer's patch macrophage derived - dendritic cells
    Levi H C Makala
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada cho, Hokkaido, Japan
    J Vet Med Sci 65:491-9. 2003
    ..Taken together, these results may provide a better understanding of the complex network regulating mucosal immune responses...
  8. ncbi Viability and infectivity of Cryptosporidium parvum oocysts detected in river water in Hokkaido, Japan
    Yoshinori Tsushima
    National Research Center for Protozoan Diseases, Obihiro University, Hokkaido, Japan
    J Vet Med Sci 65:585-9. 2003
    ..parvum genotype 2. Moreover, our data on the continued isolation, detection and identification of the C. parvum isolates is consistent with the available epidemiological data for the Tokachi area...
  9. ncbi A comparison of the phenotype of dendritic cells derived from discrete Peyer's patch macrophages of non-infected and Toxoplasma gondii infected mice
    Levi H C Makala
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan
    J Vet Med Sci 65:591-7. 2003
    ..Taken together, these results may help clarify the role of DC in the complex network regulating surface membrane antigens, as well as, their capacity for antigen uptake, processing and presentation during toxoplasmosis...
  10. ncbi A role for balance of interferon-gamma and interleukin-4 production in protective immunity against Neospora caninum infection
    Yoshifumi Nishikawa
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada cho, Obihiro, Hokkaido 080 8555, Japan
    Vet Parasitol 116:175-84. 2003
    ..caninum-infected and IFN-gamma-treated cells. In the light of the balance of Th1/Th2-type cytokine production, an IFN-gamma/IL-4 balance may have a crucial role for the control of cellular responses against the parasite invasion...
  11. ncbi Characterisation of Toxoplasma gondii engineered to express mouse interferon-gamma
    Yoshifumi Nishikawa
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada cho, Obihiro, Hokkaido 080 8555, Japan
    Int J Parasitol 33:1525-35. 2003
    ..gondii expression system may provide a new tool for cytokine gene expression and that parasites engineered to express a cytokine gene may be rationally designed for use in studies on immune responses to T. gondii...
  12. ncbi SAG1 is a host-targeted antigen for protection against toxoplasma gondii infection
    S Seng
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan
    Pathobiology 71:144-51. 2004
    ..These results suggest that SAG1 plays a critical role in eliciting protection, hence a target antigen for the development of protective Th1-based responses against T. gondii infection in mice...
  13. pmc Type 1 diabetes patients have significantly lower frequency of plasmacytoid dendritic cells in the peripheral blood
    Xueqin Chen
    Center for Biotechnology and Genomic Medicine, Medical College of Georgia, 1120 15th Street, CA4124, Augusta, GA, USA
    Clin Immunol 129:413-8. 2008
    ..These results indicate that the frequency of blood pDC and the pDC/mDC1 ratio are negatively associated with T1D...
  14. pmc APC dysfunction is correlated with defective suppression of T cell proliferation in human type 1 diabetes
    Yulan Jin
    Center for Biotechnology and Genomic Medicine, Medical College of Georgia, Augusta, GA 30912, USA
    Clin Immunol 130:272-9. 2009
    ..001) and CD4(+)CD25(hi) (p=0.009) T cells in young T1D subjects than age-matched controls but slightly lower CD4(+)CD25(+) (p=0.003) and CD4(+)CD25(hi) (p=0.08) T cells in old T1D subjects than age-matched controls...
  15. pmc Leishmania major attenuates host immunity by stimulating local indoleamine 2,3-dioxygenase expression
    Levi H C Makala
    Immunotherapy Center, Medical College of Georgia, Augusta, GA 30912, USA
    J Infect Dis 203:715-25. 2011
    ..Thus, IDO acts as a molecular switch regulating host responses, and IDO inhibitor drugs are a potential new approach to enhance host immunity to established leishmania infections...
  16. ncbi Phenotype and function of murine peritoneal cavity macrophage derived-dendritic cells
    Levi H C Makala
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada cho, Hokkaido, Japan
    J Vet Med Sci 64:813-20. 2002
    ..The generated DC cells efficiently presented soluble protein antigen to CD3(+) spleen T cells...
  17. ncbi Diagnosis of equine piroplasmosis in Xinjiang province of China by the enzyme-linked immunosorbent assays using recombinant antigens
    Xuenan Xuan
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada cho, Obihiro, Hokkaido 080 8555, Japan
    Vet Parasitol 108:179-82. 2002
    ..To our knowledge, this is the first report describing a survey on equine piroplasmosis in Xinjiang province, China...
  18. ncbi Development and evaluation of an enzyme-linked immunosorbent assay with recombinant SAG2 for diagnosis of Toxoplasma gondii infection in cats
    X Huang
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada cho, Obihiro, Hokkaido 080 8555, Japan
    J Parasitol 88:804-7. 2002
    ..All the 150 ELISA-negative samples were negative by LAT. These results indicate that the ELISA with rSAG2 expressed in E. coli should be a useful method for detection of T. gondii infection in cats...
  19. pmc In the absence of endogenous gamma interferon, mice acutely infected with Neospora caninum succumb to a lethal immune response characterized by inactivation of peritoneal macrophages
    Y Nishikawa
    National Research Center for Protozoan Diseases, Obihiro University, Obihiro, Hokkaido 080 8555, Japan
    Clin Diagn Lab Immunol 8:811-6. 2001
    ..The present study indicates that early IFN-gamma production has a crucial role in the activation of peritoneal macrophages for the induction of protective immune responses against N. caninum...
  20. ncbi Ontogeny of pig discrete Peyer's patches: expression of surface antigens
    L H Makala
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan
    J Vet Med Sci 63:625-36. 2001
    ..This implies a degree of organization of immunological cells within the discrete PP, enhancing the potential to mount immune responses in the most efficient manner...
  21. ncbi Ontogeny of pig discrete Peyer's patches: distribution and morphometric analysis
    L H Makala
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada cho, Obihiro, Hokkaido 080 8555, Japan
    Pathobiology 68:275-82. 2000
    ....
  22. pmc Characterization of Neospora caninum surface protein NcSRS2 based on baculovirus expression system and its application for serodiagnosis of Neospora infection
    Y Nishikawa
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan
    J Clin Microbiol 39:3987-91. 2001
    ..caninum from IFAT-negative sera. These results indicate that the ELISA using baculovirus-expressed NcSRS2 can be useful for effective and reliable serodiagnosis of N. caninum infection...
  23. ncbi Diagnosis of equine piroplasmosis in Brazil by serodiagnostic methods with recombinant antigens
    X Xuan
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan
    J Vet Med Sci 63:1159-60. 2001
    ..equi and B. caballi infections. These results indicate that equine piroplasmosis is widespread and therefore a cause for serious concern in the States of Sao Paulo and Mato Grosso do Sul, Brazil...
  24. ncbi Antigen presentation by murine peritoneal cavity macrophage-derived dendritic cells
    L H Makala
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido, Japan
    Pathobiology 69:104-12. 2001
    ..This accessory function of PEC-DC is antigen specific...
  25. ncbi Comparison of the accessory activity of murine peritoneal cavity macrophage derived dendritic cells and peritoneal cavity macrophages in a mixed lymphocyte reaction
    L H Makala
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan
    J Vet Med Sci 63:1271-7. 2001
    ..These variations may be attributed to the intensity of expression of MHC class II glycoproteins, as well as the actual numbers of MHC class II+ cells...
  26. ncbi Immune responses of interferon gamma (IFN-gamma) knock out mice to repeated Haemaphysalis longicornis (Acari: Ixodidae) nymph infestations
    Badgar Battsetseg
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan
    J Med Entomol 39:173-6. 2002
    ....
  27. ncbi Dendritic cells: a specialized complex system of antigen presenting cells
    Levi H C Makala
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan
    J Vet Med Sci 64:181-93. 2002
    ..The history and current status of DC termed "directors of the immune system orchestra" is reviewed. The present understanding of DC cell biology, function and use, taking into account their complexity is discussed...
  28. ncbi Canine herpesvirus ORF2 is a membrane protein modified by N-linked glycosylation
    Yoshifumi Nishikawa
    Department of Global Agricultural Science, Graduate School of Agricultural and Life Science, The University of Tokyo, Japan
    Virus Res 87:1-9. 2002
    ..In vitro, the ORF2 protein did not affect viral replication and cell-to-cell viral spreading. Present findings represent the first evidence pointing to the CHV ORF2 as a membrane protein modified by an N-linked glycosylation...
  29. ncbi Mechanisms of apoptosis in murine fibroblasts by two intracellular protozoan parasites, Toxoplasma gondii and Neospora caninum
    Yoshifumi Nishikawa
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, Japan
    Parasite Immunol 24:347-54. 2002
    ..gondii and N. caninum-infected cells. These results suggest that the Fas/FasL pathway may play a crucial role in the induction of apoptosis in N. caninum- and T. gondii-infected cells mediated by IFN-gamma...