The basic science of the subchondral boneHenning Madry
Institute for Experimental Orthopaedics, Department of Orthopaedic Surgery, Saarland University Medical Center, Homburg, Germany
Knee Surg Sports Traumatol Arthrosc 18:419-33. 2010
....
- Transplanted articular chondrocytes co-overexpressing IGF-I and FGF-2 stimulate cartilage repair in vivo
Patrick Orth
Experimental Orthopaedics and Osteoarthritis Research, Saarland University, Kirrbergerstrasse, Building 37, 66421, Homburg, Germany
Knee Surg Sports Traumatol Arthrosc 19:2119-30. 2011
..Here, the hypothesis that co-overexpression of IGF-I and FGF-2 by transplanted articular chondrocytes enhances the early repair of cartilage defects in vivo and protects the neighbouring cartilage from degeneration was tested...
- Reliability, reproducibility, and validation of five major histological scoring systems for experimental articular cartilage repair in the rabbit model
Patrick Orth
Experimental Orthopaedics and Osteoarthritis Research, Saarland University, Homburg Saar, Germany
Tissue Eng Part C Methods 18:329-39. 2012
..Histological and biochemical evaluations may serve as complementary tools to assess articular cartilage repair in vivo...
Efficacy of cationic liposome-mediated gene transfer to mesangial cells in vitro and in vivoH Madry
Department of Orthopaedic Surgery, Saarland University Medical Center, Saarland University, Homburg, Germany
J Mol Med (Berl) 79:184-9. 2001
..The current limitation of the evaluated liposomes for an efficient in vivo gene transfer to mesangial cells is the toxicity upon intrarenal arterial administration...
- [Autologous chondrocyte transplantation]
H Madry
Klinik fur Orthopadie und Orthopadische Chirurgie, Universitat des Saarlandes, Kirrberger Strasse 1, 66421, Homburg
Orthopade 37:756-63. 2008
..Long-term studies are needed to determine the effect of ACT on the development of osteoarthritis...
- [Tissue engineering for articular cartilage repair improved by gene transfer. Current concepts]
H Madry
Labor für Experimentelle Orthopädie, Klinik fur Orthopadie und Orthopadische Chirurgie, Universitatsklinikum des Saarlandes, 66421, Homburg
Orthopade 36:236-47. 2007
..The combination of cartilage tissue engineering with overexpression of potential therapeutic genes using gene transfer technologies provides a basis for the development of novel molecular therapies for the repair of cartilage defects...
- [Gene therapy in orthopaedic surgery]
H Madry
Labor für Experimentelle Orthopädie Klinik für Orthopädie und Orthopädische Chirurgie, Universitatsklinikum des Saarlandes, 66421, Homburg
Orthopade 35:1193-202; quiz 1203. 2006
..Current data indicate that gene-based therapies are effective in promoting the repair of articular cartilage, and bone defects. Such strategies may lead to the development of novel molecular therapies treatments in orthopaedic surgery...
- Nasu-Hakola disease (PLOSL): report of five cases and review of the literature
Henning Madry
Department of Orthopaedic Surgery, Saarland University Medical Center, Homburg Saar, Germany
Clin Orthop Relat Res 454:262-9. 2007
..A biopsy is not needed to confirm the diagnosis of polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy because of the unique combination of radiographic and neurologic features...
- Acceleration of articular cartilage repair by combined gene transfer of human insulin-like growth factor I and fibroblast growth factor-2 in vivo
Henning Madry
Institute for Experimental Orthopaedics and Department of Orthopaedic Surgery, Saarland University Medical Center, Homburg, Germany
Arch Orthop Trauma Surg 130:1311-22. 2010
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- Menisci are efficiently transduced by recombinant adeno-associated virus vectors in vitro and in vivo
Henning Madry
Laboratory for Experimental Orthopaedics, Department of Orthopaedic Surgery, Saarland University, Homburg, Germany
Am J Sports Med 32:1860-5. 2004
..Gene transfer is a potential approach to enhancing meniscal repair. Recombinant adeno-associated virus is a method of gene transfer that has advantages over previously used approaches to this problem...
- [Gene transfer in cruciate ligament surgery. Natural science-based principles and possible clinical applications]
H Madry
Labor für Experimentelle Orthopädie, Orthopadische Universitats und Poliklinik, Universitatskliniken des Saarlandes, 66421 Homburg
Orthopade 31:799-809. 2002
..Special characteristics that dictate the experimental strategies will be outlined...
- Overexpression of human fibroblast growth factor 2 stimulates cell proliferation in an ex vivo model of articular chondrocyte transplantation
Henning Madry
Department of Orthopaedic Surgery, Saarland University Medical Center, Homburg, Germany
J Gene Med 6:238-45. 2004
..In the present study, we tested the hypothesis that human FGF-2 (hFGF-2) gene transfer into articular chondrocytes modulates cell proliferation in an ex vivo model of chondrocyte transplantation...
- Sustained transgene expression in cartilage defects in vivo after transplantation of articular chondrocytes modified by lipid-mediated gene transfer in a gel suspension delivery system
Henning Madry
Laboratory for Experimental Orthopaedics, Department of Orthopaedic Surgery, Saarland University Medical Center, Homburg, Germany
J Gene Med 5:502-9. 2003
..We tested the hypothesis that a recombinant gene can be delivered to sites of cartilage damage in vivo using chondrocytes transfected by a lipid-mediated gene transfer method...
- Recombinant adeno-associated virus vectors efficiently and persistently transduce chondrocytes in normal and osteoarthritic human articular cartilage
Henning Madry
Orthopedic Research Laboratories, Department of Orthopedic Surgery, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02115, USA
Hum Gene Ther 14:393-402. 2003
..Finally, the data demonstrate that these rAAV vectors are capable of effectively delivering recombinant genes to chondral and osteochondral defects in vivo...
- Effective, safe nonviral gene transfer to preserve the chondrogenic differentiation potential of human mesenchymal stem cells
Sebastian Elsler
Center of Experimental Orthopaedics, Saarland University Medical Center, Saarland University, Homburg Saar, Germany
J Gene Med 14:501-11. 2012
....
- Improved tissue repair in articular cartilage defects in vivo by rAAV-mediated overexpression of human fibroblast growth factor 2
Magali Cucchiarini
Laboratory for Experimental Orthopaedics, Department of Orthopaedics and Orthopaedic Surgery, Saarland University Medical Center, D 66421 Homburg, Germany
Mol Ther 12:229-38. 2005
..This process may further benefit from cosupplementation with other factors. These results provide a basis for rAAV application to sites of articular cartilage damage to deliver agents that promote tissue repair...
- Local stimulation of articular cartilage repair by transplantation of encapsulated chondrocytes overexpressing human fibroblast growth factor 2 (FGF-2) in vivo
Gunter Kaul
Laboratory for Experimental Orthopaedics, Department of Orthopaedic Surgery, Saarland University Medical Center, Homburg, Germany
J Gene Med 8:100-11. 2006
..These results may lead to the development of safe gene-based therapies for human articular cartilage defects...
- In vitro and in vivo characterization of nonbiomedical- and biomedical-grade alginates for articular chondrocyte transplantation
Susanne Heiligenstein
Experimental Orthopaedics and Osteoarthritis Research, Saarland University, Homburg, Germany
Tissue Eng Part C Methods 17:829-42. 2011
..The individual characteristics of these alginates may be valuable to tailor specific experimental and clinical strategies for cartilage tissue engineering...
- Metabolic activities and chondrogenic differentiation of human mesenchymal stem cells following recombinant adeno-associated virus-mediated gene transfer and overexpression of fibroblast growth factor 2
Magali Cucchiarini
Experimental Orthopaedics and Osteoarthritis Research, Saarland University Medical Center, Saarland University, Homburg Saar, Germany
Tissue Eng Part A 17:1921-33. 2011
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- Human mesenchymal stem cells overexpressing therapeutic genes: from basic science to clinical applications for articular cartilage repair
Magali Cucchiarini
Center of Experimental Orthopaedics, Saarland University Medical Center, University of the Saarland, Homburg Saar, Germany
Biomed Mater Eng 22:197-208. 2012
..Here, we propose an overview of the current approaches employed for cell- and gene-based treatment of articular cartilage disorders using mesenchymal stem cells...
Benefits of recombinant adeno-associated virus (rAAV)-mediated insulinlike growth factor I (IGF-I) overexpression for the long-term reconstruction of human osteoarthritic cartilage by modulation of the IGF-I axisAnja Weimer
Center of Experimental Orthopaedics, Saarland University Medical Center, Homburg Saar, Germany
Mol Med 18:346-58. 2012
..These findings show the benefits of directly providing an IGF-I sequence to articular cartilage via rAAV for the future treatment of human osteoarthritis...
- Remodelling of human osteoarthritic cartilage by FGF-2, alone or combined with Sox9 via rAAV gene transfer
Magali Cucchiarini
Laboratory for Experimental Orthopaedics, Department of Orthopaedic Surgery, Saarland University Medical Center, Homburg, Germany
J Cell Mol Med 13:2476-88. 2009
..These data provide further motivation to develop coupled gene transfer approaches via rAAV for the treatment of human OA...
- Restoration of the extracellular matrix in human osteoarthritic articular cartilage by overexpression of the transcription factor SOX9
Magali Cucchiarini
Saarland University Medical Center, Homburg, Germany
Arthritis Rheum 56:158-67. 2007
..The purpose of this study was to investigate the ability of rAAV-mediated SOX9 overexpression to restore major ECM components in human OA articular cartilage...
- Evaluation of nonbiomedical and biomedical grade alginates for the transplantation of genetically modified articular chondrocytes to cartilage defects in a large animal model in vivo
Susanne Heiligenstein
Experimental Orthopaedics and Osteoarthritis Research, Saarland University Medical Center, Homburg, Germany
J Gene Med 13:230-42. 2011
..In the present study, we tested the hypothesis that the alginate system allows for sustained transgene expression in cartilage defects in a preclinical large animal model in vivo...
- Analysis of novel nonviral gene transfer systems for gene delivery to cells of the musculoskeletal system
Patrick Orth
Laboratory for Experimental Orthopaedics, Department of Orthopaedic Surgery, Saarland University Medical Center, Homburg, Germany
Mol Biotechnol 38:137-44. 2008
..These results indicate that relatively high levels of transfection can be achieved using novel nonviral gene transfer methods...
- Gene therapy for cartilage defects
Magali Cucchiarini
Laboratory for Experimental Orthopaedics, Department of Orthopaedic Surgery, Saarland University Medical Center, 66421 Homburg/Saar, Germany
J Gene Med 7:1495-509. 2005
..The first experimental steps provide reason for cautious optimism...
- Effect of open wedge high tibial osteotomy on the lateral compartment in sheep. Part I: analysis of the lateral meniscus
Henning Madry
Center of Experimental Orthopaedics, Saarland University, Kirrbergerstr 1, Bldg 37, 66421, Homburg, Saar, Germany
Knee Surg Sports Traumatol Arthrosc 21:39-48. 2013
..To evaluate whether medial open wedge high tibial osteotomy (HTO) results in structural and biochemical changes in the lateral meniscus in adult sheep...
- rAAV Vectors as Safe and Efficient Tools for the Stable Delivery of Genes to Primary Human Chondrosarcoma Cells In Vitro and In Situ
Henning Madry
Center of Experimental Orthopaedics, Saarland University Medical Center, Saarland, 66421 Homburg, Germany
Sarcoma 2012:347417. 2012
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- Clinical potential and challenges of using genetically modified cells for articular cartilage repair
Henning Madry
Experimental Orthopaedics and Osteoarthritis Research, Saarland University Medical Center, Homburg Saar, Germany
Croat Med J 52:245-61. 2011
..This, in turn, may provide an important step toward the unanswered question of articular cartilage regeneration...
- Effect of subchondral drilling on the microarchitecture of subchondral bone: analysis in a large animal model at 6 months
Patrick Orth
Center of Experimental Orthopaedics, Saarland University, Homburg, Germany
Am J Sports Med 40:828-36. 2012
..Marrow stimulation techniques such as subchondral drilling are clinically important treatment options for symptomatic small cartilage defects. Little is known about whether they induce deleterious changes in the subchondral bone...
- Failed cartilage repair for early osteoarthritis defects: a biochemical, histological and immunohistochemical analysis of the repair tissue after treatment with marrow-stimulation techniques
Gunter Kaul
Experimental Orthopaedics and Osteoarthritis Research, Saarland University Medical Center and Saarland University, Kirrbergerstr 1, Bldg 37, 66421, Homburg Saar, Germany
Knee Surg Sports Traumatol Arthrosc 20:2315-24. 2012
..To examine the entire repair tissue resulting from marrow-stimulation techniques in patients with early osteoarthritis...
- Biological aspects of early osteoarthritis
Henning Madry
Experimental Orthopaedics and Osteoarthritis Research, Saarland University, Kirrbergerstrasse, Building 37, 66421, Homburg, Germany
Knee Surg Sports Traumatol Arthrosc 20:407-22. 2012
..The aim of this review is to highlight the molecular basis and histopathological features of early OA...
- Gene transfer of a human insulin-like growth factor I cDNA enhances tissue engineering of cartilage
Henning Madry
Department of Orthopedic Surgery, Harvard Medical School, Massachusetts General Hospital, Boston 02114, USA
Hum Gene Ther 13:1621-30. 2002
....
- Enhanced repair of articular cartilage defects in vivo by transplanted chondrocytes overexpressing insulin-like growth factor I (IGF-I)
H Madry
Laboratory for Experimental Orthopaedics, Department of Orthopaedic Surgery, Saarland University, Homburg, Germany
Gene Ther 12:1171-9. 2005
..These results suggest that therapeutic growth factor gene delivery using encapsulated and transplanted genetically modified chondrocytes may be applicable to sites of focal articular cartilage damage...
- rAAV-mediated overexpression of FGF-2 promotes cell proliferation, survival, and alpha-SMA expression in human meniscal lesions
M Cucchiarini
Department of Orthopaedic Surgery, Laboratory for Experimental Orthopaedics, Saarland University Medical Center, Homburg Saar, Germany
Gene Ther 16:1363-72. 2009
..Such a direct gene-based approach may have value in options aiming at treating human meniscal defects...
- [Molecular tools to remodel osteoarthritic articular cartilage : growth, transcription, and signaling factors]
M Cucchiarini
Labor für Experimentelle Orthopädie, Klinik fur Orthopadie und Orthopadische Chirurgie, Universitatsklinikum des Saarlandes, Kirrbergerstrasse 37, 66421, Homburg Saar, Deutschland
Orthopade 38:1063-70. 2009
..In conclusion, recent experimental findings permit cautious optimism, holding promise for treating human OA in the future...
- Overexpression of human insulin-like growth factor-I promotes new tissue formation in an ex vivo model of articular chondrocyte transplantation
H Madry
Orthopaedic Research Laboratories, Department of Orthopaedic Surgery, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA
Gene Ther 8:1443-9. 2001
..The data further suggest that therapeutic growth factor gene transfer may be applicable to articular cartilage...
- Enamel matrix derivative inhibits proteoglycan production and articular cartilage repair, delays the restoration of the subchondral bone and induces changes of the synovial membrane in a lapine osteochondral defect model in vivo
Alice Kiss
Department of Operative Dentistry and Periodontology, Saarland University Medical Centre, Homburg Saar, Germany
J Tissue Eng Regen Med . 2012
..These data suggest that EMD inhibits the early repair of the osteochondral unit in vivo. Copyright © 2012 John Wiley & Sons, Ltd...
- Cartilage repair and joint preservation: medical and surgical treatment options
Henning Madry
Lehrstuhl für Experimentelle Orthopädie und Arthroseforschung, Universitat des Saarlandes, 66421 Homburg, Germany
Dtsch Arztebl Int 108:669-77. 2011
..Reconstructive surgical treatment strategies aim to form a repair tissue or to unload compartments of the joint with articular cartilage damage...
- Direct FGF-2 Gene Transfer via Recombinant Adeno-Associated Virus Vectors Stimulates Cell Proliferation, Collagen Production, and the Repair of Experimental Lesions in the Human ACL
Henning Madry
Magali Cucchiarini, Center of Experimental Orthopaedics, Saarland University Medical Center, Kirrbergerstr Building 37, 66421 Homburg, Germany
Am J Sports Med 41:194-202. 2013
..Basic fibroblast growth factor (FGF-2) is a powerful stimulator of fibroblast proliferation and type I/III collagen production...
- Genetic modification of mesenchymal stem cells for cartilage repair
Magali Cucchiarini
Experimental Orthopaedics and Osteoarthritis Research, Saarland University Medical Center, Homburg Saar, Germany
Biomed Mater Eng 20:135-43. 2010
..The aim of this report is to provide an overview of the most recent therapeutic approaches developed in experimental orthopaedic research...
- The subchondral bone in articular cartilage repair: current problems in the surgical management
Andreas H Gomoll
Department of Orthopaedic Surgery, Brigham and Women s Hospital, Harvard Medical School, Chestnut Hill, MA 02467, USA
Knee Surg Sports Traumatol Arthrosc 18:434-47. 2010
..Lastly, since this area remains in constant evolution, the requirements for prospective studies needed to evaluate these emerging technologies will be reviewed...
- Gene therapy for articular cartilage repair
S Trippel
Department of Orthopaedic Surgery, Indiana University School of Medicine, 541 Clinical Drive, Suite 600, Indianapolis, IP 46202 5111, USA
Proc Inst Mech Eng H 221:451-9. 2007
..Although preliminary, data from in-vitro and in-vivo studies suggest that gene therapy can deliver such potentially therapeutic agents to protect existing cartilage and to build new cartilage...
- A low morbidity surgical approach to the sheep femoral trochlea
Patrick Orth
Center of Experimental Orthopaedics and Osteoarthritis Research, Saarland University, Homburg Saar, Germany
BMC Musculoskelet Disord 14:5. 2013
..abstract:..
