- Allelic discrimination using fluorogenic probes and the 5' nuclease assayK J Livak
PE Applied Biosystems, Foster City, CA 94404, USA
Genet Anal 14:143-9. 1999..Furthermore, a standard method of analysis has been developed that enables automated genotype determination. Applications of this assay have included typing a number of polymorphisms in human drug metabolism genes...
- Polymorphisms in the human DNA ligase I gene (LIG1) including a complex GT repeatK J Livak
Research and Development Division, The Du Pont Merck Pharmaceutical, Wilmington, DE 19880 0328, USA
Mutat Res 406:1-8. 1998..Detection of the repeated sequences required the development of specialized cloning and PCR conditions. Analysis of a pedigree showed that this complex repeat is polymorphic...
- Seven-color, homogeneous detection of six PCR productsL G Lee
PE Biosystems, Foster City, CA, USA
Biotechniques 27:342-9. 1999..5) proved that the cross-talk between the dyes was minimal. SNP analyses of known, synthetic target sequences and genomic DNA were plotted both as normalized, subtracted spectra and as data points in three separate dot plots...