Use of tuf as a target for sequence-based identification of Gram-positive cocci of the genus Enterococcus, Streptococcus, coagulase-negative Staphylococcus, and LactococcusXuerui Li
State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Grazing Animal Diseases of the Ministry of Agriculture, China
Ann Clin Microbiol Antimicrob 11:31. 2012
..It is useful to simultaneously identify species in different genera using a single primer pair...
Bioinformatics analysis of rabbit haemorrhagic disease virus genomeXiao Ting Tian
State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujia ping 1, Yanchang bu, Lanzhou, Gansu, China
Virol J 8:494. 2011
..Recent researches revealed that it, as one number of the Caliciviridae, has some specialties in its genome, its reproduction and so on...
A novel bi-functional DNA vaccine expressing VP1 protein and producing antisense RNA targeted to 5'UTR of foot-and-mouth disease virus can induce both rapid inhibitory effect and specific immune response in miceBin Yang
Key Laboratory of Animal Virology of Ministry of Agriculture, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping 11, Lanzhou, Gansu 730046, PR China
Vaccine 26:5477-83. 2008
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Molecular characterization of China rabies virus vaccine strainWenqiang Jiao
State Key Laboratory of Veterinary Etiological Biology, Ministry of Agriculture, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Science, Xujia ping 1, Yanchang bu, Lanzhou, Gansu, China
Virol J 8:521. 2011
..The main goal of the present study is to amplify the whole genome of aG strain, and genetic relationships between other vaccine strains and wild strains were analyzed...
Development of a foot-and-mouth disease virus serotype A empty capsid subunit vaccine using silkworm (Bombyx mori) pupaeZhiyong Li
State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Grazing Animal Diseases of Ministry of Agriculture, Lanzhou Veterinary Research Institute, Chinese Academy of Agriculture Sciences, Lanzhou, Gansu, China
PLoS ONE 7:e43849. 2012
..33 PD(50) per dose. These data provide evidence that silkworm pupae can be used to express immunogenic FMDV proteins. This strategy might be used to develop a new generation of empty capsid subunit vaccines against a variety of diseases...
Expression and antigenicity characterization for truncated capsid protein of porcine circovirus type 2Zhongzi Lou
State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, China
Can J Vet Res 75:61-4. 2011
..Reactivity between His-XF2-2 and the 7 mAbs was detected, FmAb-8 showed the best reactivity. The dominant B-cell epitope was located at 97 to 141 aa. The PEPSCAN indicated that the P122-136 peptide contained the dominant B-cell epitope...
FMD subunit vaccine produced using a silkworm-baculovirus expression system: protective efficacy against two type Asia1 isolates in cattleZhiyong Li
State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agriculture Sciences, 1 Xujiaping, Lanzhou 730046, China
Vet Microbiol 149:99-103. 2011
..When challenged with two Chinese isolates, the subunit vaccine could achieve 6.5 PD(50) (challenged with Asia I/HNK/CHA/05 strain) and 5.2 PD(50) (challenged with Asia I/JSL/05 strain) per dose...
Molecular detection of a mixed infection of Goatpox virus, Orf virus, and Mycoplasma capricolum subsp. capripneumoniae in goatsYuefeng Chu
Key Laboratory of Grazing Animal Diseases of Ministry of Agriculture, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China
J Vet Diagn Invest 23:786-9. 2011
..The PCR products were cloned, sequenced, and aligned with related reference sequences in GenBank for further identification of the pathogens. The present study reports a mixed infection with GTPV, ORFV, and MCCP in goats...
Expression of foot-and-mouth disease virus capsid proteins in silkworm-baculovirus expression system and its utilization as a subunit vaccineZhiyong Li
Key Laboratory of Animal Virology of Ministry of Agriculture, Lanzhou Veterinary Research Institute, Chinese Academy of Agriculture Sciences, Lanzhou, Gansu, China
PLoS ONE 3:e2273. 2008
..Thus, it is urgent to develop a novel FMDV vaccine that is safer, more effective and more economical than traditional vaccines...