Arunas Lagunavicius

Summary

Country: Lithuania

Publications

  1. pmc Duality of polynucleotide substrates for Phi29 DNA polymerase: 3'-->5' RNase activity of the enzyme
    Arunas Lagunavicius
    Fermentas UAB, Graiciuno 8, Vilnius LT 02241, Lithuania
    RNA 14:503-13. 2008
  2. pmc Novel application of Phi29 DNA polymerase: RNA detection and analysis in vitro and in situ by target RNA-primed RCA
    Arunas Lagunavicius
    Fermentas UAB, Vilnius LT 02241, Lithuania
    RNA 15:765-71. 2009
  3. pmc Direct detection of RNA in vitro and in situ by target-primed RCA: The impact of E. coli RNase III on the detection efficiency of RNA sequences distanced far from the 3'-end
    Egle Merkiene
    Fermentas UAB, Graiciuno 8, Vilnius LT 02241, Lithuania
    RNA 16:1508-15. 2010
  4. doi request reprint Photocaged variants of the MunI and PvuII restriction enzymes
    Arunas Silanskas
    Institute of Biotechnology, Vilnius University, Graiciuno 8, LT 02241 Vilnius, Lithuania
    Biochemistry 50:2800-7. 2011
  5. pmc Restriction endonuclease BpuJI specific for the 5'-CCCGT sequence is related to the archaeal Holliday junction resolvase family
    Rasa Sukackaite
    Institute of Biotechnology, Graiciuno 8, Vilnius, Lithuania
    Nucleic Acids Res 35:2377-89. 2007
  6. doi request reprint Site-directed chemical modification of archaeal Thermococcus litoralis Sh1B DNA polymerase: Acquired ability to read through template-strand uracils
    Edita Gaidamaviciute
    Fermentas UAB, Vilnius LT 02241, Lithuania
    Biochim Biophys Acta 1804:1385-93. 2010
  7. doi request reprint Selective inactivation of M-MuLV RT RNase H activity by site-directed PEGylation: an improved ability to synthesize long cDNA molecules
    Tomas Radzvilavicius
    Thermo Fisher Scientific formerly Fermentas, Graiciuno 8, Vilnius LT 02241, Lithuania
    N Biotechnol 29:285-92. 2012
  8. ncbi request reprint MnlI--The member of H-N-H subtype of Type IIS restriction endonucleases
    Edita Kriukiene
    Institute of Biotechnology, Graiciuno 8, Vilnius LT 02241, Lithuania
    Biochim Biophys Acta 1751:194-204. 2005
  9. doi request reprint Efficient gene transfection using novel cationic polymers poly(hydroxyalkylene imines)
    Lolita Zaliauskiene
    Thermo Fisher Scientific formely Fermentas, Graiciuno 8, Vilnius, Lithuania
    Bioconjug Chem 21:1602-11. 2010
  10. ncbi request reprint The metal-independent type IIs restriction enzyme BfiI is a dimer that binds two DNA sites but has only one catalytic centre
    Arunas Lagunavicius
    Institute of Biotechnology, Graiciuno 8, Vilnius 2028, Lithuania
    J Mol Biol 326:1051-64. 2003

Collaborators

  • Ceslovas Venclovas
  • Alfred Pingoud
  • Edita Kriukiene
  • Saulius Grazulis
  • Edita Gaidamaviciute
  • Tomas Radzvilavicius
  • Arunas Silanskas
  • Lolita Zaliauskiene
  • Egle Merkiene
  • Laurynas Riauba
  • Claus Urbanke
  • Virginijus Siksnys
  • Rasa Sukackaite
  • Wolfgang Wende
  • Michael Foss
  • Arvydas Janulaitis
  • Sarunas Zigmantas
  • Ieva Volungeviciene
  • Daiva Tauraite
  • Ausvydas Vareikis
  • Agne Petuskaite
  • Julius Gagilas
  • Ricardas Makuska
  • Ula Bernadisiute
  • Kornelijus Stankevicius

Detail Information

Publications11

  1. pmc Duality of polynucleotide substrates for Phi29 DNA polymerase: 3'-->5' RNase activity of the enzyme
    Arunas Lagunavicius
    Fermentas UAB, Graiciuno 8, Vilnius LT 02241, Lithuania
    RNA 14:503-13. 2008
    ....
  2. pmc Novel application of Phi29 DNA polymerase: RNA detection and analysis in vitro and in situ by target RNA-primed RCA
    Arunas Lagunavicius
    Fermentas UAB, Vilnius LT 02241, Lithuania
    RNA 15:765-71. 2009
    ..We demonstrate that the exoribonucleolytic activity of Phi29 DNA polymerase can be successfully applied in vitro and in situ. These findings expand the potential for detection and analysis of RNA sequences distanced from 3'-end...
  3. pmc Direct detection of RNA in vitro and in situ by target-primed RCA: The impact of E. coli RNase III on the detection efficiency of RNA sequences distanced far from the 3'-end
    Egle Merkiene
    Fermentas UAB, Graiciuno 8, Vilnius LT 02241, Lithuania
    RNA 16:1508-15. 2010
    ..These observations extend the target RNA-primed RCA possibilities to test RNA sequences distanced far from the 3'-end and customize this technique for the inner RNA sequence analysis...
  4. doi request reprint Photocaged variants of the MunI and PvuII restriction enzymes
    Arunas Silanskas
    Institute of Biotechnology, Vilnius University, Graiciuno 8, LT 02241 Vilnius, Lithuania
    Biochemistry 50:2800-7. 2011
    ..Upon removal of the caging group by UV irradiation, nearly 50% of the catalytic activity of MunI and 80% of the catalytic activity of PvuII could be restored...
  5. pmc Restriction endonuclease BpuJI specific for the 5'-CCCGT sequence is related to the archaeal Holliday junction resolvase family
    Rasa Sukackaite
    Institute of Biotechnology, Graiciuno 8, Vilnius, Lithuania
    Nucleic Acids Res 35:2377-89. 2007
    ..This leads to a complicated pattern of specific DNA cleavage in the vicinity of the target site. Bioinformatics analysis identifies the AHJR-like domain in the putative Type III enzymes and functionally uncharacterized proteins...
  6. doi request reprint Site-directed chemical modification of archaeal Thermococcus litoralis Sh1B DNA polymerase: Acquired ability to read through template-strand uracils
    Edita Gaidamaviciute
    Fermentas UAB, Vilnius LT 02241, Lithuania
    Biochim Biophys Acta 1804:1385-93. 2010
    ..We demonstrate that the acquired resistance of chemically modified T. litoralis Sh1B DNA polymerase to DNA uracil correlates with its decreased affinity for template-strand uracil...
  7. doi request reprint Selective inactivation of M-MuLV RT RNase H activity by site-directed PEGylation: an improved ability to synthesize long cDNA molecules
    Tomas Radzvilavicius
    Thermo Fisher Scientific formerly Fermentas, Graiciuno 8, Vilnius LT 02241, Lithuania
    N Biotechnol 29:285-92. 2012
    ..As a consequence, the efficiency of long cDNA molecules synthesis by modified enzyme was greatly increased...
  8. ncbi request reprint MnlI--The member of H-N-H subtype of Type IIS restriction endonucleases
    Edita Kriukiene
    Institute of Biotechnology, Graiciuno 8, Vilnius LT 02241, Lithuania
    Biochim Biophys Acta 1751:194-204. 2005
    ..We interpret the presented experimental evidence as a suggestion that the motif 306Rx3ExHHx14Nx8H represents the active site of MnlI. Consequentially, MnlI seems to be the member of Type IIS with the active site of the H-N-H type...
  9. doi request reprint Efficient gene transfection using novel cationic polymers poly(hydroxyalkylene imines)
    Lolita Zaliauskiene
    Thermo Fisher Scientific formely Fermentas, Graiciuno 8, Vilnius, Lithuania
    Bioconjug Chem 21:1602-11. 2010
    ..In summary, the results indicate that pHP is a new efficient reagent for gene delivery in vitro and in vivo...
  10. ncbi request reprint The metal-independent type IIs restriction enzyme BfiI is a dimer that binds two DNA sites but has only one catalytic centre
    Arunas Lagunavicius
    Institute of Biotechnology, Graiciuno 8, Vilnius 2028, Lithuania
    J Mol Biol 326:1051-64. 2003
    ..Yet, when bound to two copies of its recognition sequence, the BfiI dimer cleaved only one phosphodiester bond at a time. The dimer thus seems to contain two DNA-binding domains but only one active site...
  11. pmc Crystal structure of the Bse634I restriction endonuclease: comparison of two enzymes recognizing the same DNA sequence
    Saulius Grazulis
    Max Planck Institut fur Biochemie, Abt Strukturforschung, Am Klopferspitz 18a, D 82152 Martinsried bei munchen, Germany
    Nucleic Acids Res 30:876-85. 2002
    ..Thus, restriction enzymes Bse634I, Cfr10I and NgoMIV, recognizing overlapping nucleotide sequences, exhibit a conserved tetrameric architecture that is of functional importance...