Research Topics
| Arunas LagunaviciusSummaryCountry: Lithuania Publications
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Detail Information
Publications
Duality of polynucleotide substrates for Phi29 DNA polymerase: 3'-->5' RNase activity of the enzymeArunas Lagunavicius
Fermentas UAB, Graiciuno 8, Vilnius LT 02241, Lithuania
RNA 14:503-13. 2008....- Novel application of Phi29 DNA polymerase: RNA detection and analysis in vitro and in situ by target RNA-primed RCAArunas Lagunavicius
Fermentas UAB, Vilnius LT 02241, Lithuania
RNA 15:765-71. 2009..We demonstrate that the exoribonucleolytic activity of Phi29 DNA polymerase can be successfully applied in vitro and in situ. These findings expand the potential for detection and analysis of RNA sequences distanced from 3'-end... - Direct detection of RNA in vitro and in situ by target-primed RCA: The impact of E. coli RNase III on the detection efficiency of RNA sequences distanced far from the 3'-endEgle Merkiene
Fermentas UAB, Graiciuno 8, Vilnius LT 02241, Lithuania
RNA 16:1508-15. 2010..These observations extend the target RNA-primed RCA possibilities to test RNA sequences distanced far from the 3'-end and customize this technique for the inner RNA sequence analysis... 
Photocaged variants of the MunI and PvuII restriction enzymesArunas Silanskas
Institute of Biotechnology, Vilnius University, Graiciuno 8, LT 02241 Vilnius, Lithuania
Biochemistry 50:2800-7. 2011..Upon removal of the caging group by UV irradiation, nearly 50% of the catalytic activity of MunI and 80% of the catalytic activity of PvuII could be restored...- Restriction endonuclease BpuJI specific for the 5'-CCCGT sequence is related to the archaeal Holliday junction resolvase familyRasa Sukackaite
Institute of Biotechnology, Graiciuno 8, Vilnius, Lithuania
Nucleic Acids Res 35:2377-89. 2007..This leads to a complicated pattern of specific DNA cleavage in the vicinity of the target site. Bioinformatics analysis identifies the AHJR-like domain in the putative Type III enzymes and functionally uncharacterized proteins... - Site-directed chemical modification of archaeal Thermococcus litoralis Sh1B DNA polymerase: Acquired ability to read through template-strand uracilsEdita Gaidamaviciute
Fermentas UAB, Vilnius LT 02241, Lithuania
Biochim Biophys Acta 1804:1385-93. 2010..We demonstrate that the acquired resistance of chemically modified T. litoralis Sh1B DNA polymerase to DNA uracil correlates with its decreased affinity for template-strand uracil... - Selective inactivation of M-MuLV RT RNase H activity by site-directed PEGylation: an improved ability to synthesize long cDNA moleculesTomas Radzvilavicius
Thermo Fisher Scientific formerly Fermentas, Graiciuno 8, Vilnius LT 02241, Lithuania
N Biotechnol 29:285-92. 2012..As a consequence, the efficiency of long cDNA molecules synthesis by modified enzyme was greatly increased... 
MnlI--The member of H-N-H subtype of Type IIS restriction endonucleasesEdita Kriukiene
Institute of Biotechnology, Graiciuno 8, Vilnius LT-02241, Lithuania
Biochim Biophys Acta 1751:194-204. 2005..We interpret the presented experimental evidence as a suggestion that the motif 306Rx3ExHHx14Nx8H represents the active site of MnlI. Consequentially, MnlI seems to be the member of Type IIS with the active site of the H-N-H type...- Efficient gene transfection using novel cationic polymers poly(hydroxyalkylene imines)Lolita Zaliauskiene
Thermo Fisher Scientific formely Fermentas, Graiciuno 8, Vilnius, Lithuania
Bioconjug Chem 21:1602-11. 2010..In summary, the results indicate that pHP is a new efficient reagent for gene delivery in vitro and in vivo... - The metal-independent type IIs restriction enzyme BfiI is a dimer that binds two DNA sites but has only one catalytic centreArunas Lagunavicius
Institute of Biotechnology, Graiciuno 8, Vilnius 2028, Lithuania
J Mol Biol 326:1051-64. 2003..Yet, when bound to two copies of its recognition sequence, the BfiI dimer cleaved only one phosphodiester bond at a time. The dimer thus seems to contain two DNA-binding domains but only one active site... - Crystal structure of the Bse634I restriction endonuclease: comparison of two enzymes recognizing the same DNA sequenceSaulius Grazulis
Max Planck Institut fur Biochemie, Abt Strukturforschung, Am Klopferspitz 18a, D 82152 Martinsried bei munchen, Germany
Nucleic Acids Res 30:876-85. 2002..Thus, restriction enzymes Bse634I, Cfr10I and NgoMIV, recognizing overlapping nucleotide sequences, exhibit a conserved tetrameric architecture that is of functional importance...