Research Topics


Shi Lung Lin



  1. Lin S, Ying S. Mechanism and Method for Generating Tumor-Free iPS Cells Using Intronic MicroRNA miR-302 Induction. Methods Mol Biol. 2018;1733:265-282 pubmed publisher
    ..In this chapter, we review the mechanism underlying miR-302-mediated tumor suppression and then demonstrate how to apply this mechanism to generate tumor-free iPSCs. The same strategy may also be used to prevent ESC tumorigenicity. ..
  2. Lin S. microRNAs and Fragile X Syndrome. Adv Exp Med Biol. 2015;888:107-21 pubmed publisher
  3. Lin S, Ying S. Gene Silencing In Vitro and In Vivo Using Intronic MicroRNAs. Methods Mol Biol. 2018;1733:107-126 pubmed publisher
    ..These miRNA-mediated animal models provide artificial means to reproduce the mechanisms of miRNA-induced disease in vivo and will shed further light on miRNA-related therapies. ..
  4. Lin S, Chang D, Ying S. Isolation and Identification of Gene-Specific MicroRNAs. Methods Mol Biol. 2018;1733:173-180 pubmed publisher
    ..According to this proof-of-principle methodology, we now have full knowledge to design various intronic pre-miRNA inserts that are more efficient and effective for inducing specific gene silencing effects in vitro and in vivo. ..
  5. Lin S, Chang S, Ying S. Transgene-Like Animal Models Using Intronic MicroRNAs. Methods Mol Biol. 2018;1733:239-254 pubmed publisher
  6. Lin S. Identification and Isolation of Novel Sugar-Like RNA Protecting Materials: Glycylglycerins from Pluripotent Stem Cells. Methods Mol Biol. 2018;1733:305-316 pubmed publisher
  7. Lin S, Chang D, Ying S, Leu D, Wu D. MicroRNA miR-302 inhibits the tumorigenecity of human pluripotent stem cells by coordinate suppression of the CDK2 and CDK4/6 cell cycle pathways. Cancer Res. 2010;70:9473-82 pubmed publisher
    ..Overall, our findings indicate that miR-302 inhibits human pluripotent stem cell tumorigenicity by enhancing multiple G1 phase arrest pathways rather than by silencing p21Cip1. ..
  8. Chang Lin S, HUNG A, Chang D, Lin Y, Ying S, Lin S. Novel glycylated sugar alcohols protect ESC-specific microRNAs from degradation in iPS cells. Nucleic Acids Res. 2016;44:4894-906 pubmed publisher