Du L, Dong S, Zhang X, Jiang C, Chen J, Yao L, et al
. Selective oxidation of aliphatic C-H bonds in alkylphenols by a chemomimetic biocatalytic system. Proc Natl Acad Sci U S A. 2017;114:E5129-E5137 pubmed publisher
..Moreover, the crystal structures of the central P450 biocatalyst CreJ in complex with two representative substrates provide significant structural insights into its substrate flexibility and reaction selectivity. ..
Qi F, Lei C, Li F, Zhang X, Wang J, Zhang W, et al
. Deciphering the late steps of rifamycin biosynthesis. Nat Commun. 2018;9:2342 pubmed publisher
..Both reactions showcase interesting chemistries for these two widespread and well-studied enzyme families. ..
Du L, Ma L, Qi F, Zheng X, Jiang C, Li A, et al
. Characterization of a Unique Pathway for 4-Cresol Catabolism Initiated by Phosphorylation in Corynebacterium glutamicum. J Biol Chem. 2016;291:6583-94 pubmed publisher
..Steady-state kinetic parameters (Km and kcat) for each catabolic step were determined, and these results suggest that kinetic controls serve a key role in directing the metabolic flux to the most energy effective route. ..
Chen H, Meng X, Xu X, Liu W, Li S. The molecular basis for lipase stereoselectivity. Appl Microbiol Biotechnol. 2018;102:3487-3495 pubmed publisher
..Moreover, the synergistic effects of these structural factors on the catalytic properties including stereoselectivity, activity, and stability are also discussed. ..
Sun Y, Ma L, Han D, Du L, Qi F, Zhang W, et al
. In vitro reconstitution of the cyclosporine specific P450 hydroxylases using heterologous redox partner proteins. J Ind Microbiol Biotechnol. 2017;44:161-166 pubmed publisher
..The results suggest the great value of cyanobacterial redox partner proteins for both academic research and industrial application of P450 biocatalysts. ..
Li Z, Du L, Zhang W, Zhang X, Jiang Y, Liu K, et al
. Complete elucidation of the late steps of bafilomycin biosynthesis in Streptomyces lohii. J Biol Chem. 2017;292:7095-7104 pubmed publisher
..Taken together, this work provides significant insights into bafilomycin biosynthesis to inform future pharmacological development of these compounds. ..
Zhang X, Li S. Expansion of chemical space for natural products by uncommon P450 reactions. Nat Prod Rep. 2017;34:1061-1089 pubmed publisher
..These uncommon P450 reactions lead to a higher level of chemical space expansion for natural products, through which a broader spectrum of bioactivities can be gained by the host organisms. ..
Zhang W, Huffman J, Li S, Shen Y, Du L. Unusual acylation of chloramphenicol in Lysobacter enzymogenes, a biocontrol agent with intrinsic resistance to multiple antibiotics. BMC Biotechnol. 2017;17:59 pubmed publisher
..Because Lysobacter are ubiquitous inhabitants of soil and water, the finding may have important implications in understanding microbial competitions and bioactive natural product regulation. ..