Benjamin Levi

Summary

Publications

  1. pmc Role of Indian hedgehog signaling in palatal osteogenesis
    Benjamin Levi
    Hagey Laboratory for Pediatric Regenerative Medicine, Department of Surgery, Plastic and Reconstructive Surgery Division, Stanford University School of Medicine, Stanford, Calif, USA
    Plast Reconstr Surg 127:1182-90. 2011
  2. pmc Deleterious effects of freezing on osteogenic differentiation of human adipose-derived stromal cells in vitro and in vivo
    Aaron W James
    Hagey Pediatric Regenerative Research Laboratory, Plastic and Reconstructive Surgery Division, Department of Surgery, Stanford University School of Medicine, Stanford, California 94305 5148, USA
    Stem Cells Dev 20:427-39. 2011
  3. pmc Role of GSK-3β in the osteogenic differentiation of palatal mesenchyme
    Emily R Nelson
    Hagey Laboratory for Pediatric Regenerative Medicine, Plastic and Reconstructive Surgery Division, Department of Surgery, Stanford University School of Medicine, Stanford, California, United States of America
    PLoS ONE 6:e25847. 2011
  4. doi request reprint Dura mater stimulates human adipose-derived stromal cells to undergo bone formation in mouse calvarial defects
    Benjamin Levi
    Hagey Laboratory for Pediatric Regenerative Medicine, Plastic and Reconstructive Surgery Division, Department of Surgery, Stanford University School of Medicine, Stanford, California 94305 5148, USA
    Stem Cells 29:1241-55. 2011
  5. doi request reprint Nonintegrating knockdown and customized scaffold design enhances human adipose-derived stem cells in skeletal repair
    Benjamin Levi
    Hagey Laboratory for Pediatric Regenerative Medicine, Department of Surgery, Plastic and Reconstructive Surgery Division, Stanford University School of Medicine, Stanford, California 94305 5148, USA
    Stem Cells 29:2018-29. 2011
  6. pmc Human adipose-derived stromal cells stimulate autogenous skeletal repair via paracrine Hedgehog signaling with calvarial osteoblasts
    Benjamin Levi
    Hagey Laboratory for Pediatric Regenerative Medicine, Plastic and Reconstructive Surgery Division, Department of Surgery, Stanford University School of Medicine, Stanford, California 94305 5148, USA
    Stem Cells Dev 20:243-57. 2011
  7. pmc Acute skeletal injury is necessary for human adipose-derived stromal cell-mediated calvarial regeneration
    Benjamin Levi
    Hagey Laboratory for Pediatric Regenerative Medicine, Department of Surgery, Plastic and Reconstructive Surgery Division, Stanford University School of Medicine, Stanford, Calif 94305 5148, USA
    Plast Reconstr Surg 127:1118-29. 2011
  8. doi request reprint Novel application of human morphomics to quantify temporal soft tissues in Pierre Robin and Treacher Collins
    Jeffrey Lisiecki
    Department of Surgery, Section of Plastic Surgery, University of Michigan, Ann Arbor, Michigan, USA
    J Craniofac Surg 24:158-62. 2013
  9. doi request reprint Molecular analysis and differentiation capacity of adipose-derived stem cells from lymphedema tissue
    Benjamin Levi
    Department of Surgery, Division of Plastic and Reconstructive Surgery, Stanford University School of Medicine, 257 Campus Drive West, Hagey Building, Stanford, CA 94305 5148, USA
    Plast Reconstr Surg 132:580-9. 2013
  10. pmc In vivo directed differentiation of pluripotent stem cells for skeletal regeneration
    Benjamin Levi
    Hagey Laboratory for Pediatric Regenerative Medicine, Department of Surgery, Plastic and Reconstructive Surgery Division, Stanford University School of Medicine, Stanford, CA 94305, USA
    Proc Natl Acad Sci U S A 109:20379-84. 2012

Collaborators

Detail Information

Publications38

  1. pmc Role of Indian hedgehog signaling in palatal osteogenesis
    Benjamin Levi
    Hagey Laboratory for Pediatric Regenerative Medicine, Department of Surgery, Plastic and Reconstructive Surgery Division, Stanford University School of Medicine, Stanford, Calif, USA
    Plast Reconstr Surg 127:1182-90. 2011
    ..The authors hypothesize that Indian hedgehog activity plays a role in osteogenesis within the secondary palate and that defects in this pathway may inhibit osteogenesis of the secondary palate...
  2. pmc Deleterious effects of freezing on osteogenic differentiation of human adipose-derived stromal cells in vitro and in vivo
    Aaron W James
    Hagey Pediatric Regenerative Research Laboratory, Plastic and Reconstructive Surgery Division, Department of Surgery, Stanford University School of Medicine, Stanford, California 94305 5148, USA
    Stem Cells Dev 20:427-39. 2011
    ..The use of recombinant proteins, however, may be used to mitigate the deleterious effects of the freeze-thaw process...
  3. pmc Role of GSK-3β in the osteogenic differentiation of palatal mesenchyme
    Emily R Nelson
    Hagey Laboratory for Pediatric Regenerative Medicine, Plastic and Reconstructive Surgery Division, Department of Surgery, Stanford University School of Medicine, Stanford, California, United States of America
    PLoS ONE 6:e25847. 2011
    ..Using GSK-3ß null mouse embryos, we examine the potential coordinate roles of Wnt and Hedgehog signaling on palatal ossification...
  4. doi request reprint Dura mater stimulates human adipose-derived stromal cells to undergo bone formation in mouse calvarial defects
    Benjamin Levi
    Hagey Laboratory for Pediatric Regenerative Medicine, Plastic and Reconstructive Surgery Division, Department of Surgery, Stanford University School of Medicine, Stanford, California 94305 5148, USA
    Stem Cells 29:1241-55. 2011
    ..DM is an osteogenic cell type that both participates in and stimulates osseous healing in a hASC-engrafted calvarial defect. Furthermore, DM-derived BMP-2 paracrine stimulation appears to play a key role for hASC mediated repair...
  5. doi request reprint Nonintegrating knockdown and customized scaffold design enhances human adipose-derived stem cells in skeletal repair
    Benjamin Levi
    Hagey Laboratory for Pediatric Regenerative Medicine, Department of Surgery, Plastic and Reconstructive Surgery Division, Stanford University School of Medicine, Stanford, California 94305 5148, USA
    Stem Cells 29:2018-29. 2011
    ..This study therefore suggests that genetic targeting of hASCs combined with custom scaffold design can optimize hASCs for skeletal regenerative medicine...
  6. pmc Human adipose-derived stromal cells stimulate autogenous skeletal repair via paracrine Hedgehog signaling with calvarial osteoblasts
    Benjamin Levi
    Hagey Laboratory for Pediatric Regenerative Medicine, Plastic and Reconstructive Surgery Division, Department of Surgery, Stanford University School of Medicine, Stanford, California 94305 5148, USA
    Stem Cells Dev 20:243-57. 2011
    ..Our studies suggest that hASC-derived Hedgehog signaling may play a paracrine role in skeletal repair...
  7. pmc Acute skeletal injury is necessary for human adipose-derived stromal cell-mediated calvarial regeneration
    Benjamin Levi
    Hagey Laboratory for Pediatric Regenerative Medicine, Department of Surgery, Plastic and Reconstructive Surgery Division, Stanford University School of Medicine, Stanford, Calif 94305 5148, USA
    Plast Reconstr Surg 127:1118-29. 2011
    ..The more clinically relevant repair of an established skeletal defect, however, has not been addressed. The authors sought to determine whether human ASCs could heal chronic (established) calvarial defects...
  8. doi request reprint Novel application of human morphomics to quantify temporal soft tissues in Pierre Robin and Treacher Collins
    Jeffrey Lisiecki
    Department of Surgery, Section of Plastic Surgery, University of Michigan, Ann Arbor, Michigan, USA
    J Craniofac Surg 24:158-62. 2013
    ..We posit that the temporalis muscle and temporal fat pad, like other derivatives of the first branchial arch, are hypoplastic in patients with TC and PR compared to age-matched controls...
  9. doi request reprint Molecular analysis and differentiation capacity of adipose-derived stem cells from lymphedema tissue
    Benjamin Levi
    Department of Surgery, Division of Plastic and Reconstructive Surgery, Stanford University School of Medicine, 257 Campus Drive West, Hagey Building, Stanford, CA 94305 5148, USA
    Plast Reconstr Surg 132:580-9. 2013
    ..Liposuction to address the adipose nature of the lymphedema has provided an opportunity for a detailed analysis of the stromal fraction of lymphedema-associated fat to clarify the molecular mechanisms for this adipogenic transformation...
  10. pmc In vivo directed differentiation of pluripotent stem cells for skeletal regeneration
    Benjamin Levi
    Hagey Laboratory for Pediatric Regenerative Medicine, Department of Surgery, Plastic and Reconstructive Surgery Division, Stanford University School of Medicine, Stanford, CA 94305, USA
    Proc Natl Acad Sci U S A 109:20379-84. 2012
    ....
  11. pmc CD105 protein depletion enhances human adipose-derived stromal cell osteogenesis through reduction of transforming growth factor β1 (TGF-β1) signaling
    Benjamin Levi
    Hagey Laboratory for Pediatric Regenerative Medicine, Department of Surgery, Plastic and Reconstructive Surgery Division, Stanford University School of Medicine, Stanford, California 94305, USA
    J Biol Chem 286:39497-509. 2011
    ..These findings thus highlight a potential avenue to promote osteogenesis in adipose-derived mesenchymal cells for skeletal regeneration...
  12. pmc Enhancement of human adipose-derived stromal cell angiogenesis through knockdown of a BMP-2 inhibitor
    Benjamin Levi
    Hagey Laboratory for Pediatric Regenerative Medicine, Department of Surgery, Plastic and Reconstructive Surgery Division, Stanford University School of Medicine, Stanford, Calif 94305 5148, USA
    Plast Reconstr Surg 129:53-66. 2012
    ....
  13. pmc Regulation of human adipose-derived stromal cell osteogenic differentiation by insulin-like growth factor-1 and platelet-derived growth factor-alpha
    Benjamin Levi
    Hagey Pediatric Regenerative Research Laboratory, Department of Surgery, Plastic and Reconstructive Surgery Division, Stanford University School of Medicine, Stanford, Calif 94305 5148, USA
    Plast Reconstr Surg 126:41-52. 2010
    ..The authors hypothesized that insulin-like growth factor (IGF) and platelet-derived growth factor (PDGF) would stimulate human adipose-derived stromal cell osteogenesis and that IGF would stimulate adipogenesis...
  14. doi request reprint Differences in osteogenic differentiation of adipose-derived stromal cells from murine, canine, and human sources in vitro and in vivo
    Benjamin Levi
    Hagey Pediatric Regenerative Medicine Research Laboratory, Department of Surgery, Plastic and Reconstructive Surgery Division, Stanford University School of Medicine, Stanford, CA 94305 5148, USA
    Plast Reconstr Surg 128:373-86. 2011
    ..Briefly, the authors found that significant differences exist with regard to proliferation and osteogenic potentials of adipose-derived stromal cells across species...
  15. pmc Studies in adipose-derived stromal cells: migration and participation in repair of cranial injury after systemic injection
    Benjamin Levi
    Hagey Pediatric Regenerative Research Laboratory, Department of Surgery, Plastic and Reconstructive Surgery Division, Stanford University School of Medicine, Stanford, Calif 94305 5148, USA
    Plast Reconstr Surg 127:1130-40. 2011
    ..The authors sought to examine whether systemically administered adipose-derived stromal cells would migrate to and heal surgically created defects of the mouse cranial skeleton...
  16. doi request reprint Divergent modulation of adipose-derived stromal cell differentiation by TGF-beta1 based on species of derivation
    Benjamin Levi
    Hagey Pediatric Regenerative Research Laboratory, Department of Surgery, Division of Plastic and Reconstructive Surgery, Stanford University School of Medicine, Stanford, CA 94305 5148, USA
    Plast Reconstr Surg 126:412-25. 2010
    ..In the following study, the authors sought to determine the species-specific response of adipose-derived stromal cells to recombinant TGF-beta1 (rTGF-beta1)...
  17. pmc Human adipose derived stromal cells heal critical size mouse calvarial defects
    Benjamin Levi
    Hagey Pediatric Regenerative Research Laboratory, Plastic and Reconstructive Surgery Division, Department of Surgery, Stanford University School of Medicine, Stanford, California, USA
    PLoS ONE 5:e11177. 2010
    ..This suggests that they may be used to heal defects of the craniofacial or appendicular skeleton. We sought to substantiate the use of undifferentiated hASCs in the regeneration of a non-healing mouse skeletal defect...
  18. doi request reprint Temporal morphomics as a model for determining preoperative risk of blood transfusion in nonsyndromic craniosynostosis patients
    Jacob Rinkinen
    Department of Surgery, Section of Plastic Surgery, University of Michigan, Ann Arbor, MI, USA
    Plast Reconstr Surg 132:403e-12e. 2013
    ..The authors propose an objective model, based on patients' three-dimensional anatomical characteristics, to stratify the likelihood of needing perioperative packed red blood cells...
  19. pmc Micro-computed tomography evaluation of human fat grafts in nude mice
    Michael T Chung
    Hagey Laboratory for Pediatric Regenerative Medicine, Plastic and Reconstructive Surgery Division, Department of Surgery, Stanford University School of Medicine, California, USA
    Tissue Eng Part C Methods 19:227-32. 2013
    ..The present study evaluated the long-term viability of human fat grafts in a murine model using a novel imaging technique allowing for in vivo volumetric analysis...
  20. doi request reprint Cranial suture biology: from pathways to patient care
    Benjamin Levi
    Hagey Laboratory of Pediatric Regenerative Medicine Research, Department of Surgery, Plastic and Reconstructive Surgery Division, Stanford University School of Medicine, Stanford, California 94305, USA
    J Craniofac Surg 23:13-9. 2012
    ..In this review, we discuss the development of the cranial vault and explain the basic science behind craniosynostosis in humans as well as in animal models and how these studies may lead to future advances in craniosynostosis treatments...
  21. pmc Paracrine interaction between adipose-derived stromal cells and cranial suture-derived mesenchymal cells
    Aaron W James
    Hagey Laboratory for Pediatric Regenerative Medicine, Department of Surgery, Plastic and Reconstructive Surgery Division, Stanford University School of Medicine, Stanford, Calif, USA
    Plast Reconstr Surg 126:806-21. 2010
    ..Various reports have previously examined the unique in vitro attributes of suture-derived mesenchymal cells; this study sought to extend those findings...
  22. pmc Stem cells: update and impact on craniofacial surgery
    Benjamin Levi
    From the Hagey Laboratory for Pediatric Regenerative Medicine Research Laboratory and Plastic and Reconstructive Surgery Division, Department of Surgery, and Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, Stanford, California
    J Craniofac Surg 23:319-23. 2012
    ....
  23. pmc Preclinical derivation and imaging of autologously transplanted canine induced pluripotent stem cells
    Andrew S Lee
    Department of Radiology, Stanford University School of Medicine, Stanford, California 94305 5454, USA
    J Biol Chem 286:32697-704. 2011
    ..To demonstrate clinical potential of ciPSCs to treat models of injury, we generated endothelial cells (ciPSC-ECs) and used these cells to treat immunodeficient murine models of myocardial infarction and hindlimb ischemia...
  24. doi request reprint Depot-specific variation in the osteogenic and adipogenic potential of human adipose-derived stromal cells
    Benjamin Levi
    Hagey Pediatric Regenerative Medicine Research Laboratory, Department of Surgery, Plastic and Reconstructive Surgery Division, Stanford University School of Medicine, Stanford University, Stanford, Calif 94305 5148, USA
    Plast Reconstr Surg 126:822-34. 2010
    ..However, multiple facets of their biology remain unclear. The authors examined the variations in osteogenesis and adipogenesis in adipose-derived stromal cells between subcutaneous fat depots and potential molecular causes...
  25. pmc Sonic Hedgehog influences the balance of osteogenesis and adipogenesis in mouse adipose-derived stromal cells
    Aaron W James
    Hagey Pediatric Regenerative Medicine Laboratory, Division of Plastic and Reconstructive Surgery, Department of Surgery, Stanford University School of Medicine, Stanford, California 94305 5148, USA
    Tissue Eng Part A 16:2605-16. 2010
    ..These data suggest that Hedgehog signaling directs the lineage differentiation of mesodermal stem cells and represents a promising strategy for skeletal tissue regeneration...
  26. pmc Palatogenesis: engineering, pathways and pathologies
    Benjamin Levi
    Hagey Laboratory for Pediatric Regenerative Medicine, Department of Surgery, Plastic and Reconstructive Surgery Division, Stanford University School of Medicine, Stanford, California, USA
    Organogenesis 7:242-54. 2011
    ....
  27. ncbi request reprint Elucidating mechanisms of osteogenesis in human adipose-derived stromal cells via microarray analysis
    Jackie Lee
    Department of Surgery, Division of Plastic and Reconstructive Surgery, Stanford University School of Medicine, Stanford, California, USA
    J Craniofac Surg 21:1136-41. 2010
    ..Subsequently, we analyzed those genes related to osteogenesis that have not been previously studied about hASCs. We have preliminarily assessed the role of IGFBP3, TGF-B3, TNC, CTGF, DKK-1, and PDGFRB in hASC osteogenic differentiation...
  28. doi request reprint Novel temporalis muscle and fat pad morphomic analyses aids preoperative risk evaluation and outcome assessment in nonsyndromic craniosynostosis
    Jacob Rinkinen
    Section of Plastic Surgery, Department of Surgery, University of Michigan, Ann Arbor, MI 48109 0219, USA
    J Craniofac Surg 24:250-5. 2013
    ....
  29. doi request reprint Fat grafting for thermal injury: current state and future directions
    Kavitha Ranganathan
    Department of Surgery, Section of Plastic Surgery, University of Michigan, Ann Arbor, MI, USA
    J Burn Care Res 34:219-26. 2013
    ....
  30. pmc Pierre Robin sequence and Treacher Collins hypoplastic mandible comparison using three-dimensional morphometric analysis
    Michael T Chung
    Hagey Laboratory for Pediatric Regenerative Medicine, Department of Surgery, Plastic and Reconstructive Surgery Division, Stanford University School of Medicine, Stanford, CA 94305, USA
    J Craniofac Surg 23:1959-63. 2012
    ..These findings underscore distinct considerations that must be made in surgical planning for reconstruction...
  31. pmc Strategies for organ level tissue engineering
    Kristine C Rustad
    Stanford University, Hagey Laboratory for Pediatric Regenerative Medicine, Stanford, CA, USA
    Organogenesis 6:151-7. 2010
    ..Ultimately, the successful translation of tissue-engineered constructs into everyday clinical practice will depend upon the ability of the tissue engineer to "scale up" every aspect of the research and development process...
  32. pmc Concise review: adipose-derived stromal cells for skeletal regenerative medicine
    Benjamin Levi
    Hagey Pediatric Regenerative Medicine Research Laboratory, Department of Surgery, Plastic and Reconstructive Surgery Division, Stanford University School of Medicine, Stanford, California 94305 5148, USA
    Stem Cells 29:576-82. 2011
    ....
  33. pmc Adipose-derived mesenchymal stem cells from ventral hernia repair patients demonstrate decreased vasculogenesis
    Jeffrey Lisiecki
    Department of Surgery, University of Michigan, 1500 E Medical Center Drive, Ann Arbor, MI 48109, USA
    Biomed Res Int 2014:983715. 2014
    ..Ventral hernia repair (VHR) patients are at high risk for wound infections. We hypothesize that ASCs from VHR patients are less vasculogenic than ASCs from healthy controls...
  34. doi request reprint Outcomes of tethered cord repair with a layered soft tissue closure
    Benjamin Levi
    Section of Plastic Surgery, Department of Surgery, University of Michigan, Ann Arbor, MI, USA
    Ann Plast Surg 70:74-8. 2013
    ..We propose that composite tissue closure of tethered cord repairs yields superior outcomes and that a collaborative effort between neurosurgery and plastic surgery may result in enhanced structural and functional results...
  35. doi request reprint Heterotopic ossification following burn injury: the role of stem cells
    Emily R Nelson
    Department of Surgery, Plastic and Reconstructive Surgery Division, Hagey Laboratory for Pediatric Regenerative Medicine, Stanford University School of Medicine, Stanford, California, USA
    J Burn Care Res 33:463-70. 2012
    ..Current literature supports a role for MSCs in modulating heterotopic bone formation, and direct manipulation of MSCs might one day be used to prevent and treat HO...
  36. pmc Stem cell niches for skin regeneration
    Victor W Wong
    Department of Surgery, Oregon Health and Science University, 3181 Southwest Sam Jackson Park Road, Portland, OR 97239, USA
    Int J Biomater 2012:926059. 2012
    ..Innovative biomaterial systems that successfully recapitulate these microenvironments will facilitate progenitor cell-mediated skin repair and regeneration...
  37. pmc Retinoic acid enhances osteogenesis in cranial suture-derived mesenchymal cells: potential mechanisms of retinoid-induced craniosynostosis
    Aaron W James
    Hagey Pediatric Regenerative Research Laboratory, Department of Surgery, Division of Plastic and Reconstructive Surgery, Stanford University School of Medicine, Stanford, CA, USA
    Plast Reconstr Surg 125:1352-61. 2010
    ..The authors used the culture of mouse cranial suture-derived mesenchymal cells to probe the potential cellular mechanisms of this teratogen to better elucidate mechanisms of retinoid-induced suture fusion...
  38. doi request reprint The basic science of vascular biology: implications for the practicing surgeon
    Jason P Glotzbach
    Hagey Laboratory for Pediatric Regenerative Medicine, Division of Plastic and Reconstructive Surgery, Department of Surgery, Stanford University School of Medicine, Stanford, CA 94305 5148, USA
    Plast Reconstr Surg 126:1528-38. 2010
    ....