Elissa P Lei

Summary

Publications

  1. Chen D, Brovkina M, Matzat L, Lei E. Shep RNA-Binding Capacity Is Required for Antagonism of gypsy Chromatin Insulator Activity. G3 (Bethesda). 2019;9:749-754 pubmed publisher
    ..Our findings suggest that regulation of insulator function by RNA-binding proteins relies on RNA-mediated interactions. ..
  2. Chen D, Lei E. Function and regulation of chromatin insulators in dynamic genome organization. Curr Opin Cell Biol. 2019;58:61-68 pubmed publisher
    ..Insulator-dependent structures vary across individual cells and tissues, highlighting the need to study the regulation of insulators in particular temporal and spatial contexts throughout development. ..
  3. Bag I, Dale R, Palmer C, Lei E. The zinc-finger protein CLAMP promotes gypsy chromatin insulator function in Drosophila. J Cell Sci. 2019;132: pubmed publisher
    ..Finally, reduction of CLAMP disrupts CP190 localization within the nucleus. Our results support a positive functional relationship between CLAMP and CP190 to promote gypsy chromatin insulator activity. ..
  4. Názer E, Dale R, Palmer C, Lei E. Argonaute2 attenuates active transcription by limiting RNA Polymerase II elongation in Drosophila melanogaster. Sci Rep. 2018;8:15685 pubmed publisher
    ..Altogether, these results provide key insights into the molecular role of AGO2 in attenuating elongation of certain actively transcribed genes. ..
  5. Caravaca J, Lei E. Maintenance of a Drosophila melanogaster Population Cage. J Vis Exp. 2016;: pubmed publisher
    ..This paper describes a detailed and flexible protocol for the maintenance of a Drosophila melanogaster population cage, starting with 1.5 g of harvested material from the previous cycle. ..
  6. Matzat L, Lei E. Surviving an identity crisis: a revised view of chromatin insulators in the genomics era. Biochim Biophys Acta. 2014;1839:203-14 pubmed publisher
    ..Finally, we discuss the emerging understanding of mechanisms of chromatin insulator regulation. This article is part of a Special Issue entitled: Chromatin and epigenetic regulation of animal development. ..
  7. Názer E, Lei E. Modulation of chromatin modifying complexes by noncoding RNAs in trans. Curr Opin Genet Dev. 2014;25:68-73 pubmed publisher
    ..In this review, we discuss new insights into the mechanisms by which ncRNAs function in trans with respect to Polycomb Group, chromatin insulator, and dosage compensation complexes in mammals and/or Drosophila. ..
  8. King M, Matzat L, Dale R, Lim S, Lei E. The RNA-binding protein Rumpelstiltskin antagonizes gypsy chromatin insulator function in a tissue-specific manner. J Cell Sci. 2014;127:2956-66 pubmed publisher
    ..Our findings indicate parallel roles for RNA-binding proteins in mediating tissue-specific regulation of chromatin insulator activity. ..
  9. Dale R, Matzat L, Lei E. metaseq: a Python package for integrative genome-wide analysis reveals relationships between chromatin insulators and associated nuclear mRNA. Nucleic Acids Res. 2014;42:9158-70 pubmed publisher
    ..Based on the metaseq-enabled analysis presented here, we propose a model where Shep associates with chromatin cotranscriptionally, then is recruited to insulator complexes in trans where it plays a negative role in insulator activity. ..

More Information

Publications11

  1. Chinen M, Lei E. Drosophila Argonaute2 turnover is regulated by the ubiquitin proteasome pathway. Biochem Biophys Res Commun. 2017;483:951-957 pubmed publisher
    ..Finally, AGO2 protein levels increase after treatment with the proteasome inhibitor MG132. Our results indicate that the ubiquitin-proteasome pathway is involved in AGO2 protein turnover. ..
  2. Chen D, Dale R, Lei E. Shep regulates Drosophila neuronal remodeling by controlling transcription of its chromatin targets. Development. 2018;145: pubmed publisher
    ..Our results reveal direct regulation of transcriptional programs by Shep to regulate neuronal remodeling during metamorphosis. ..