- Single cell cytometry of protein function in RNAi treated cells and in native populations
Department of Biological Technologies, Oncology Research, Wyeth Research, 87 Cambridge Park Drive, Cambridge, MA 02140, USA
BMC Cell Biol 9:43. 2008
..Such approaches are important for immune cells analyzed by flow cytometry, but have not been broadly available for adherent cells that are critical to the study of solid-tumor cancers and other disease models...
- A kinome-wide siRNA screen identifies multiple roles for protein kinases in hypoxic stress adaptation, including roles for IRAK4 and GAK in protection against apoptosis in VHL-/- renal carcinoma cells, despite activation of the NF-κB pathway
Applied Genomics, Department of Biological Technologies, Wyeth Research, Cambridge, MA, USA
J Biomol Screen 18:782-96. 2013
- Quantitative optimization of reverse transfection conditions for 384-well siRNA library screening
Section of Biologic Research, Department of Biological Technologies, Wyeth Research, Cambridge, MA 02140, USA
Assay Drug Dev Technol 6:683-91. 2008
..The assay is fast, inexpensive, and quantitative. Conditions identified as optimal using this technique have been employed successfully in library screens...
- High-content screening moves to the front of the line
Steven A Haney
Department of Biological Technologies, Wyeth Research, 87 Cambridge Park Drive, Cambridge, MA 02140, USA
Drug Discov Today 11:889-94. 2006
..The use of HCS in target validation is expanding the work that can be done at this stage, especially the range of targets that can be characterized, and putting it into a more biological context...
- Image-based assessment of growth and signaling changes in cancer cells mediated by direct cell-cell contact
Department of Biological Technologies, Wyeth Research, Cambridge, Massachusetts, USA
PLoS ONE 4:e6822. 2009
..Despite the crucial importance of the cellular environment in regulating cellular signaling, in vitro methods for the study of such interactions are difficult and/or indirect...
- Impact of image segmentation on high-content screening data quality for SK-BR-3 cells
Andrew A Hill
Department of Biological Technologies, Wyeth Research, 35 CambridgePark Drive, Cambridge, MA 02140, USA
BMC Bioinformatics 8:340. 2007
..We asked if segmentation errors were common for a clinically relevant cell line, if such errors had measurable effects on the data, and if HCS data could be improved by automated identification of well-segmented cells...