JOHN DAVID LAMBETH

Summary

Publications

  1. ncbi request reprint Nox/Duox family of nicotinamide adenine dinucleotide (phosphate) oxidases
    J David Lambeth
    Department of Pathology, Emory University Medical School, Atlanta, Georgia 30322, USA
    Curr Opin Hematol 9:11-7. 2002
  2. ncbi request reprint NOX enzymes and the biology of reactive oxygen
    J David Lambeth
    Department of Pathology and Laboratory Medicine, Emory University Medical School, Atlanta, Georgia 30322, USA
    Nat Rev Immunol 4:181-9. 2004
  3. pmc Molecular evolution of the reactive oxygen-generating NADPH oxidase (Nox/Duox) family of enzymes
    Tsukasa Kawahara
    Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, Georgia 30322, USA
    BMC Evol Biol 7:109. 2007
  4. doi request reprint NOX enzymes as novel targets for drug development
    J David Lambeth
    Emory University, Atlanta, GA, USA
    Semin Immunopathol 30:339-63. 2008
  5. pmc Constitutive NADPH-dependent electron transferase activity of the Nox4 dehydrogenase domain
    Yukio Nisimoto
    Department of Pathology and Laboratory Medicine, Emory University Medical School, Atlanta, Georgia 30322, USA
    Biochemistry 49:2433-42. 2010
  6. pmc Molecular evolution of Phox-related regulatory subunits for NADPH oxidase enzymes
    Tsukasa Kawahara
    Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, Georgia 30322, USA
    BMC Evol Biol 7:178. 2007
  7. pmc Nox5 forms a functional oligomer mediated by self-association of its dehydrogenase domain
    Tsukasa Kawahara
    Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, Georgia 30322, United States
    Biochemistry 50:2013-25. 2011
  8. pmc Regulation of Nox and Duox enzymatic activity and expression
    J David Lambeth
    Department of Pathology and Laboratory Medicine, 148 Whitehead Biomedical Research Building, Emory University, 615 Michael Street, Atlanta, GA 30322, USA
    Free Radic Biol Med 43:319-31. 2007
  9. pmc Nox enzymes, ROS, and chronic disease: an example of antagonistic pleiotropy
    J David Lambeth
    148 Whitehead Biomedical Research Building, Department of Pathology and Laboratory Medicine, 615 Michael Street, Atlanta, GA 30322, USA
    Free Radic Biol Med 43:332-47. 2007
  10. ncbi request reprint Nox1-dependent reactive oxygen generation is regulated by Rac1
    Guangjie Cheng
    Department of Pathology and Laboratory Medicine, Emory University Medical School, 615 Michael Street, Atlanta, GA 30322, USA
    J Biol Chem 281:17718-26. 2006

Research Grants

  1. Regulation of Nox Enzymes by Calcium and Novel Subunits
    JOHN DAVID LAMBETH; Fiscal Year: 2010
  2. Regulation of Nox Enzymes by Calcium and Novel Subunits
    J Lambeth; Fiscal Year: 2007
  3. Mox 1: A Novel Mitogenic Oxidase
    J Lambeth; Fiscal Year: 2007
  4. Mox 1: A Novel Mitogenic Oxidase
    JOHN DAVID LAMBETH; Fiscal Year: 2010
  5. Mox 1: A Novel Mitogenic Oxidase
    J Lambeth; Fiscal Year: 2009
  6. MOX 1--A NOVEL MITOGENIC OXIDASE
    J Lambeth; Fiscal Year: 2006
  7. Dual Oxidases (Duox): Enzymology & Biological Function
    J Lambeth; Fiscal Year: 2006

Collaborators

  • Tsukasa Kawahara
  • Mary Dinauer
  • Kathy Griendling
  • Mark Quinn
  • J L Arbiser
  • Lula L Hilenski
  • Robert A Clark
  • David Harrison
  • Jose M Cuezva
  • Larry Oberley
  • A M Shah
  • John Hoidal
  • Jian Mei Li
  • Barry Goldstein
  • Karl Heinz Krause
  • Brian Nickoloff
  • Guangjie Cheng
  • Rebecca S Arnold
  • Darren R Ritsick
  • Yukio Nisimoto
  • Heather M Jackson
  • Anna E Dikalova
  • BERNARD LASSEGUE
  • Dan Sorescu
  • Eunice Laurent
  • Katalin Szocs
  • Sergey Dikalov
  • Baskaran Govindarajan
  • Priya Ranjan
  • John A Petros
  • Darren Ritsick
  • Anna Dikalova
  • So Dug Lim
  • Kalyankar Mahadev
  • Junji Mitsushita
  • W Robert Taylor
  • Daiana Weiss
  • Sukhdev S Brar
  • Jonathan A Byrne
  • Marianne O Price
  • Liisa Valppu
  • Susan M E Smith
  • Maria Carolina Gongora
  • Hisamitsu Ogawa
  • Roberto A Macina
  • Wenhui Liu
  • Jackie Papkoff
  • Sylvie Robine
  • James W McCoy
  • Bethaney J Vincent
  • Jeffrey A Canter
  • Victoria Finnerty
  • Meiling Li
  • Joyce Slingerland
  • Jan A Smeitink
  • William A Edens
  • Yuping Zhang
  • Qiqin Yin-Goen
  • Andrew N Young
  • James E Sligh
  • Marta Martínez-Díez
  • Cynthia Cohen
  • Milton W Datta
  • Ju He
  • Andrea Remo
  • Richard J Rodenburg
  • Yasmin Hughes
  • Vikas Anathy
  • Peter M Burch
  • Nicholas H Heintz
  • Kelly Weirather
  • Becky A Diebold
  • Gary K Owens
  • Roza Clempus
  • Leland Chung
  • David S Weber
  • Alejandra San Martin
  • Carrie Sun
  • Mahul Amin
  • Harald H H W Schmidt
  • Fray Marshall
  • Alicia Lyle
  • James McCoy
  • Jean Marie Ruddy
  • Tohru Kamata
  • Hiroyuki Motoshima
  • Xiangdong Wu
  • Thomas P Kennedy
  • Christopher Gove
  • Kristia G Ardie

Detail Information

Publications34

  1. ncbi request reprint Nox/Duox family of nicotinamide adenine dinucleotide (phosphate) oxidases
    J David Lambeth
    Department of Pathology, Emory University Medical School, Atlanta, Georgia 30322, USA
    Curr Opin Hematol 9:11-7. 2002
    ....
  2. ncbi request reprint NOX enzymes and the biology of reactive oxygen
    J David Lambeth
    Department of Pathology and Laboratory Medicine, Emory University Medical School, Atlanta, Georgia 30322, USA
    Nat Rev Immunol 4:181-9. 2004
  3. pmc Molecular evolution of the reactive oxygen-generating NADPH oxidase (Nox/Duox) family of enzymes
    Tsukasa Kawahara
    Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, Georgia 30322, USA
    BMC Evol Biol 7:109. 2007
    ....
  4. doi request reprint NOX enzymes as novel targets for drug development
    J David Lambeth
    Emory University, Atlanta, GA, USA
    Semin Immunopathol 30:339-63. 2008
    ..As most (though not all) NOX-related diseases appear to be mediated by a single member of the NOX family, agents with isoform specificity will be preferred, although broadly active NOX inhibitors may prove to be useful in some settings...
  5. pmc Constitutive NADPH-dependent electron transferase activity of the Nox4 dehydrogenase domain
    Yukio Nisimoto
    Department of Pathology and Laboratory Medicine, Emory University Medical School, Atlanta, Georgia 30322, USA
    Biochemistry 49:2433-42. 2010
    ..These results indicate that the Nox4 DH domain exists in an intrinsically activated state and that electron transfer from NADPH to FAD is likely to be rate-limiting in the NADPH-dependent reduction of oxygen by holo-Nox4...
  6. pmc Molecular evolution of Phox-related regulatory subunits for NADPH oxidase enzymes
    Tsukasa Kawahara
    Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, Georgia 30322, USA
    BMC Evol Biol 7:178. 2007
    ....
  7. pmc Nox5 forms a functional oligomer mediated by self-association of its dehydrogenase domain
    Tsukasa Kawahara
    Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, Georgia 30322, United States
    Biochemistry 50:2013-25. 2011
    ..As a result of oligomerization, the short, calcium-independent splice form, Nox5S, may function as an endogenous inhibitor of calcium-stimulated ROS generation by full-length Nox5...
  8. pmc Regulation of Nox and Duox enzymatic activity and expression
    J David Lambeth
    Department of Pathology and Laboratory Medicine, 148 Whitehead Biomedical Research Building, Emory University, 615 Michael Street, Atlanta, GA 30322, USA
    Free Radic Biol Med 43:319-31. 2007
    ..The regulation of Nox and Duox expression in tissues and by specific stimuli is also considered here. An accompanying review considers biological and pathological roles of the Nox family of enzymes...
  9. pmc Nox enzymes, ROS, and chronic disease: an example of antagonistic pleiotropy
    J David Lambeth
    148 Whitehead Biomedical Research Building, Department of Pathology and Laboratory Medicine, 615 Michael Street, Atlanta, GA 30322, USA
    Free Radic Biol Med 43:332-47. 2007
    ....
  10. ncbi request reprint Nox1-dependent reactive oxygen generation is regulated by Rac1
    Guangjie Cheng
    Department of Pathology and Laboratory Medicine, Emory University Medical School, 615 Michael Street, Atlanta, GA 30322, USA
    J Biol Chem 281:17718-26. 2006
    ..A model is presented comparing activation by regulatory subunits of Nox1 versus gp91(phox) (Nox2) in which Rac1 activation provides a major trigger that acutely activates Nox1-dependent ROS generation...
  11. ncbi request reprint Nox3 regulation by NOXO1, p47phox, and p67phox
    Guangjie Cheng
    Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, GA 30322, USA
    J Biol Chem 279:34250-5. 2004
    ..The unique regulation of Nox3 supports a model in which multiple interactions with regulatory subunits stabilize an active conformation of the catalytic subunit...
  12. ncbi request reprint Point mutations in the proline-rich region of p22phox are dominant inhibitors of Nox1- and Nox2-dependent reactive oxygen generation
    Tsukasa Kawahara
    Department of Pathology and Experimental Medicine, Emory University School of Medicine, Atlanta, Georgia 30322, USA
    J Biol Chem 280:31859-69. 2005
    ..These studies stress the importance of p22phox for the function of Nox1, Nox2, Nox3, and Nox4, and emphasize the key role of the PRR for regulating Nox proteins whose activity is dependent upon p47phox or NOXO1...
  13. ncbi request reprint NOXO1, regulation of lipid binding, localization, and activation of Nox1 by the Phox homology (PX) domain
    Guangjie Cheng
    Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, Georgia 30322, USA
    J Biol Chem 279:4737-42. 2004
    ..Thus, in transfected HEK293 cells, NOXO1 and NOXA1 activate Nox1 without the need for agonist activation, and this is mediated in part by binding of the NOXO1 PX domain to membrane lipids...
  14. pmc Phosphatidylinositol (4,5)-bisphosphate modulates Nox5 localization via an N-terminal polybasic region
    Tsukasa Kawahara
    Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, GA 30322, USA
    Mol Biol Cell 19:4020-31. 2008
    ..In contrast, mutation in PBR-C did not affect localization. Thus, extracellular ROS production by Nox5 is modulated by PtdIns(4,5)P(2) by localizing Nox5 to the plasma membrane...
  15. ncbi request reprint Distinct subcellular localizations of Nox1 and Nox4 in vascular smooth muscle cells
    Lula L Hilenski
    Division of Cardiology, Emory University School of Medicine, Atlanta, GA 30322, USA
    Arterioscler Thromb Vasc Biol 24:677-83. 2004
    ..We hypothesize that the opposing functions of Nox1 and Nox4 are reflected in their differential subcellular locations...
  16. ncbi request reprint Superoxide production and expression of nox family proteins in human atherosclerosis
    Dan Sorescu
    Department of Medicine, Emory University, Atlanta, GA 30322, USA
    Circulation 105:1429-35. 2002
    ....
  17. pmc Nox4 B-loop creates an interface between the transmembrane and dehydrogenase domains
    Heather M Jackson
    Department of Pathology and Experimental Medicine, Emory University School of Medicine, Atlanta, Georgia 30322, USA
    J Biol Chem 285:10281-90. 2010
    ..These data indicate that the B-loop is critical for Nox4 function; we propose that the B-loop, by binding to the dehydrogenase domain, provides the interface between the transmembrane and dehydrogenase domains of Nox enzymes...
  18. ncbi request reprint Nox1 overexpression potentiates angiotensin II-induced hypertension and vascular smooth muscle hypertrophy in transgenic mice
    Anna Dikalova
    Division of Cardiology, Emory University, Atlanta, GA 30322, USA
    Circulation 112:2668-76. 2005
    ..Nox1 upregulation has been implicated in cardiovascular pathologies such as hypertension and restenosis...
  19. pmc Nox regulation of smooth muscle contraction
    Darren R Ritsick
    Department of Pathology and Laboratory Medicine, Emory University, Atlanta, GA 30322, USA
    Free Radic Biol Med 43:31-8. 2007
    ..Thus, these studies demonstrate a novel biological role for Nox-generated ROS in mediating agonist-induced calcium flux and smooth muscle contraction...
  20. pmc Overexpression of Akt converts radial growth melanoma to vertical growth melanoma
    Baskaran Govindarajan
    Department of Dermatology, Emory University School of Medicine, and Atlanta Veterans Administration Medical Center, Atlanta, Georgia 30322, USA
    J Clin Invest 117:719-29. 2007
    ..Akt thus serves as a molecular switch that increases angiogenesis and the generation of superoxide, fostering more aggressive tumor behavior. Targeting Akt and ROS may be of therapeutic importance in treatment of advanced melanoma...
  21. ncbi request reprint Upregulation of Nox-based NAD(P)H oxidases in restenosis after carotid injury
    Katalin Szocs
    Department of Medicine, Emory University, Atlanta, GA 30322, USA
    Arterioscler Thromb Vasc Biol 22:21-7. 2002
    ..This dynamic regulation of oxidase components may be critical to smooth muscle phenotypic modulation in restenosis and atherosclerosis...
  22. ncbi request reprint Alternative mRNA splice forms of NOXO1: differential tissue expression and regulation of Nox1 and Nox3
    Guangjie Cheng
    Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, GA 30322, USA
    Gene 356:118-26. 2005
    ..These data suggest different tissue localizations and functions for NOXO1beta and NOXO1gamma in regulating Nox family members...
  23. ncbi request reprint Increased Nox1 and hydrogen peroxide in prostate cancer
    So Dug Lim
    Department of Pathology and Laboratory Medicine, Emory Clinic Building B, Emory University School of Medicine, Atlanta, Georgia, USA
    Prostate 62:200-7. 2005
    ..We propose that NADPH oxidases (Nox) account for increased levels of ROS in some cancers. Previously, transfection of Nox1 into a prostate cancer cell line dramatically enhanced tumor growth (Arbiser et al.: PNAS 99:715-720, 2001)...
  24. pmc Spring brings breezes, wheezes, and pollen oxidases
    Darren R Ritsick
    Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, Georgia 30322, USA
    J Clin Invest 115:2067-9. 2005
    ..These findings suggest that inhibition of the pollen oxidase may provide a way to antagonize allergic inflammation at a very early step...
  25. pmc Upregulation of Nox1 in vascular smooth muscle leads to impaired endothelium-dependent relaxation via eNOS uncoupling
    Anna E Dikalova
    Department of Medicine, Emory University, Atlanta, Georgia 30322, USA
    Am J Physiol Heart Circ Physiol 299:H673-9. 2010
    ....
  26. pmc Reactive oxygen generated by Nox1 triggers the angiogenic switch
    Jack L Arbiser
    Department of Dermatology, Emory University School of Medicine, Atlanta, GA 30322, USA
    Proc Natl Acad Sci U S A 99:715-20. 2002
    ..Nox1 induction of VEGF is eliminated by coexpression of catalase, indicating that hydrogen peroxide signals part of the switch to the angiogenic phenotype...
  27. pmc Nox1 expression determines cellular reactive oxygen and modulates c-fos-induced growth factor, interleukin-8, and Cav-1
    Rebecca S Arnold
    Department of Urology, Winship Cancer Institute, Emory University School of Medicine, 1365 Clifton Rd, Building B, Atlanta, GA 30322, USA
    Am J Pathol 171:2021-32. 2007
    ..0001). These studies indicate that Nox1 overexpression may function as a reversible signal for cellular proliferation with relevance for a common human tumor...
  28. pmc Nox1 is over-expressed in human colon cancers and correlates with activating mutations in K-Ras
    Eunice Laurent
    Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, GA 30322, USA
    Int J Cancer 123:100-7. 2008
    ..We conclude that Nox1 mRNA and protein are overexpressed in colon cancer and are strongly correlated with activating mutations in K-Ras...
  29. ncbi request reprint Creation of a genetic system for analysis of the phagocyte respiratory burst: high-level reconstitution of the NADPH oxidase in a nonhematopoietic system
    Marianne O Price
    Herman B Wells Center for Pediatric Research, Department of Pediatrics, James Whitcomb Riley Hospital for Children, Indiana University Medical Center, Indianapolis 46202, USA
    Blood 99:2653-61. 2002
    ....
  30. ncbi request reprint NOX5 NAD(P)H oxidase regulates growth and apoptosis in DU 145 prostate cancer cells
    Sukhdev S Brar
    Department of Internal Medicine, Carolinas Medical Center, Charlotte, NC 28232, USA
    Am J Physiol Cell Physiol 285:C353-69. 2003
    ..These results indicate that ROS generated by the newly described NOX5 oxidase are essential for prostate cancer growth, possibly by providing trophic intracellular oxidant tone that retards programmed cell death...
  31. ncbi request reprint Contrasting roles of NADPH oxidase isoforms in pressure-overload versus angiotensin II-induced cardiac hypertrophy
    Jonathan A Byrne
    Department of Cardiology, King s College London, London, UK
    Circ Res 93:802-5. 2003
    ..These data suggest a differential response of the cardiac Nox isoforms, gp91phox and Nox4, to Ang II versus pressure overload...
  32. ncbi request reprint Redox-dependent expression of cyclin D1 and cell proliferation by Nox1 in mouse lung epithelial cells
    Priya Ranjan
    Department of Pathology and Vermont Cancer Center, University of Vermont College of Medicine, Burlington, 05405, USA
    Antioxid Redox Signal 8:1447-59. 2006
    ....
  33. pmc The NAD(P)H oxidase homolog Nox4 modulates insulin-stimulated generation of H2O2 and plays an integral role in insulin signal transduction
    Kalyankar Mahadev
    Dorrance H Hamilton Research Laboratories, Division of Endocrinology, Diabetes and Metabolic Diseases, Department of Medicine, Jefferson Medical College, Thomas Jefferson University, Philadelphia, Pennsylvania 19107 6799, USA
    Mol Cell Biol 24:1844-54. 2004
    ....
  34. ncbi request reprint The superoxide-generating oxidase Nox1 is functionally required for Ras oncogene transformation
    Junji Mitsushita
    Department of Molecular Biology and Biochemistry, Shinshu University School of Medicine, Matsumoto, Nagano, Japan
    Cancer Res 64:3580-5. 2004
    ..Therefore, we propose that increased reactive oxygen species generation by Ras-induced Nox1 is required for oncogenic Ras transformation...

Research Grants22

  1. Regulation of Nox Enzymes by Calcium and Novel Subunits
    JOHN DAVID LAMBETH; Fiscal Year: 2010
    ..This proposal centers on understanding the fundamentals of this process, and has direct implications, for example in our ability to design new classes of drugs that treat these diseases by targeting Nox/Duox enzymes. ..
  2. Regulation of Nox Enzymes by Calcium and Novel Subunits
    J Lambeth; Fiscal Year: 2007
    ..These studies are expected to have implications with regard to both normal and aberrant generation of ROS signals relevant to cell growth and cancer. ..
  3. Mox 1: A Novel Mitogenic Oxidase
    J Lambeth; Fiscal Year: 2007
    ....
  4. Mox 1: A Novel Mitogenic Oxidase
    JOHN DAVID LAMBETH; Fiscal Year: 2010
    ....
  5. Mox 1: A Novel Mitogenic Oxidase
    J Lambeth; Fiscal Year: 2009
    ..Such inhibitors will have potential in the treatment of several types of diseases, including cancer, cardiovascular diseases, and shock lung. ..
  6. MOX 1--A NOVEL MITOGENIC OXIDASE
    J Lambeth; Fiscal Year: 2006
    ..abstract_text> ..
  7. Dual Oxidases (Duox): Enzymology & Biological Function
    J Lambeth; Fiscal Year: 2006
    ..Because ECM is a critical determinant of transformation Duox enzymes may play an important role in cancer biology in some tissues. ..