Nox/Duox family of nicotinamide adenine dinucleotide (phosphate) oxidasesJ David Lambeth
Department of Pathology, Emory University Medical School, Atlanta, Georgia 30322, USA
Curr Opin Hematol 9:11-7. 2002
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- NOX enzymes and the biology of reactive oxygen
J David Lambeth
Department of Pathology and Laboratory Medicine, Emory University Medical School, Atlanta, Georgia 30322, USA
Nat Rev Immunol 4:181-9. 2004
Molecular evolution of the reactive oxygen-generating NADPH oxidase (Nox/Duox) family of enzymesTsukasa Kawahara
Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, Georgia 30322, USA
BMC Evol Biol 7:109. 2007
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NOX enzymes as novel targets for drug developmentJ David Lambeth
Emory University, Atlanta, GA, USA
Semin Immunopathol 30:339-63. 2008
..As most (though not all) NOX-related diseases appear to be mediated by a single member of the NOX family, agents with isoform specificity will be preferred, although broadly active NOX inhibitors may prove to be useful in some settings...
- Molecular evolution of Phox-related regulatory subunits for NADPH oxidase enzymes
Tsukasa Kawahara
Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, Georgia 30322, USA
BMC Evol Biol 7:178. 2007
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- Nox5 forms a functional oligomer mediated by self-association of its dehydrogenase domain
Tsukasa Kawahara
Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, Georgia 30322, United States
Biochemistry 50:2013-25. 2011
..As a result of oligomerization, the short, calcium-independent splice form, Nox5S, may function as an endogenous inhibitor of calcium-stimulated ROS generation by full-length Nox5...
- Regulation of Nox and Duox enzymatic activity and expression
J David Lambeth
Department of Pathology and Laboratory Medicine, 148 Whitehead Biomedical Research Building, Emory University, 615 Michael Street, Atlanta, GA 30322, USA
Free Radic Biol Med 43:319-31. 2007
..The regulation of Nox and Duox expression in tissues and by specific stimuli is also considered here. An accompanying review considers biological and pathological roles of the Nox family of enzymes...
- Nox enzymes, ROS, and chronic disease: an example of antagonistic pleiotropy
J David Lambeth
148 Whitehead Biomedical Research Building, Department of Pathology and Laboratory Medicine, 615 Michael Street, Atlanta, GA 30322, USA
Free Radic Biol Med 43:332-47. 2007
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- Nox1-dependent reactive oxygen generation is regulated by Rac1
Guangjie Cheng
Department of Pathology and Laboratory Medicine, Emory University Medical School, 615 Michael Street, Atlanta, GA 30322, USA
J Biol Chem 281:17718-26. 2006
..A model is presented comparing activation by regulatory subunits of Nox1 versus gp91(phox) (Nox2) in which Rac1 activation provides a major trigger that acutely activates Nox1-dependent ROS generation...
- Nox3 regulation by NOXO1, p47phox, and p67phox
Guangjie Cheng
Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, GA 30322, USA
J Biol Chem 279:34250-5. 2004
..The unique regulation of Nox3 supports a model in which multiple interactions with regulatory subunits stabilize an active conformation of the catalytic subunit...
- Point mutations in the proline-rich region of p22phox are dominant inhibitors of Nox1- and Nox2-dependent reactive oxygen generation
Tsukasa Kawahara
Department of Pathology and Experimental Medicine, Emory University School of Medicine, Atlanta, Georgia 30322, USA
J Biol Chem 280:31859-69. 2005
..These studies stress the importance of p22phox for the function of Nox1, Nox2, Nox3, and Nox4, and emphasize the key role of the PRR for regulating Nox proteins whose activity is dependent upon p47phox or NOXO1...
- NOXO1, regulation of lipid binding, localization, and activation of Nox1 by the Phox homology (PX) domain
Guangjie Cheng
Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, Georgia 30322, USA
J Biol Chem 279:4737-42. 2004
..Thus, in transfected HEK293 cells, NOXO1 and NOXA1 activate Nox1 without the need for agonist activation, and this is mediated in part by binding of the NOXO1 PX domain to membrane lipids...
- Distinct subcellular localizations of Nox1 and Nox4 in vascular smooth muscle cells
Lula L Hilenski
Division of Cardiology, Emory University School of Medicine, Atlanta, GA 30322, USA
Arterioscler Thromb Vasc Biol 24:677-83. 2004
..We hypothesize that the opposing functions of Nox1 and Nox4 are reflected in their differential subcellular locations...
- Constitutive NADPH-dependent electron transferase activity of the Nox4 dehydrogenase domain
Yukio Nisimoto
Department of Pathology and Laboratory Medicine, Emory University Medical School, Atlanta, Georgia 30322, USA
Biochemistry 49:2433-42. 2010
..These results indicate that the Nox4 DH domain exists in an intrinsically activated state and that electron transfer from NADPH to FAD is likely to be rate-limiting in the NADPH-dependent reduction of oxygen by holo-Nox4...
- Phosphatidylinositol (4,5)-bisphosphate modulates Nox5 localization via an N-terminal polybasic region
Tsukasa Kawahara
Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, GA 30322, USA
Mol Biol Cell 19:4020-31. 2008
..In contrast, mutation in PBR-C did not affect localization. Thus, extracellular ROS production by Nox5 is modulated by PtdIns(4,5)P(2) by localizing Nox5 to the plasma membrane...
- Superoxide production and expression of nox family proteins in human atherosclerosis
Dan Sorescu
Department of Medicine, Emory University, Atlanta, GA 30322, USA
Circulation 105:1429-35. 2002
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- Nox4 B-loop creates an interface between the transmembrane and dehydrogenase domains
Heather M Jackson
Department of Pathology and Experimental Medicine, Emory University School of Medicine, Atlanta, Georgia 30322, USA
J Biol Chem 285:10281-90. 2010
..These data indicate that the B-loop is critical for Nox4 function; we propose that the B-loop, by binding to the dehydrogenase domain, provides the interface between the transmembrane and dehydrogenase domains of Nox enzymes...
- Upregulation of Nox-based NAD(P)H oxidases in restenosis after carotid injury
Katalin Szocs
Department of Medicine, Emory University, Atlanta, GA 30322, USA
Arterioscler Thromb Vasc Biol 22:21-7. 2002
..This dynamic regulation of oxidase components may be critical to smooth muscle phenotypic modulation in restenosis and atherosclerosis...
- Nox1 overexpression potentiates angiotensin II-induced hypertension and vascular smooth muscle hypertrophy in transgenic mice
Anna Dikalova
Division of Cardiology, Emory University, Atlanta, GA 30322, USA
Circulation 112:2668-76. 2005
..Nox1 upregulation has been implicated in cardiovascular pathologies such as hypertension and restenosis...
- Nox regulation of smooth muscle contraction
Darren R Ritsick
Department of Pathology and Laboratory Medicine, Emory University, Atlanta, GA 30322, USA
Free Radic Biol Med 43:31-8. 2007
..Thus, these studies demonstrate a novel biological role for Nox-generated ROS in mediating agonist-induced calcium flux and smooth muscle contraction...
- Overexpression of Akt converts radial growth melanoma to vertical growth melanoma
Baskaran Govindarajan
Department of Dermatology, Emory University School of Medicine, and Atlanta Veterans Administration Medical Center, Atlanta, Georgia 30322, USA
J Clin Invest 117:719-29. 2007
..Akt thus serves as a molecular switch that increases angiogenesis and the generation of superoxide, fostering more aggressive tumor behavior. Targeting Akt and ROS may be of therapeutic importance in treatment of advanced melanoma...
- Increased Nox1 and hydrogen peroxide in prostate cancer
So Dug Lim
Department of Pathology and Laboratory Medicine, Emory Clinic Building B, Emory University School of Medicine, Atlanta, Georgia, USA
Prostate 62:200-7. 2005
..We propose that NADPH oxidases (Nox) account for increased levels of ROS in some cancers. Previously, transfection of Nox1 into a prostate cancer cell line dramatically enhanced tumor growth (Arbiser et al.: PNAS 99:715-720, 2001)...
- Alternative mRNA splice forms of NOXO1: differential tissue expression and regulation of Nox1 and Nox3
Guangjie Cheng
Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, GA 30322, USA
Gene 356:118-26. 2005
..These data suggest different tissue localizations and functions for NOXO1beta and NOXO1gamma in regulating Nox family members...
- Nox1 is over-expressed in human colon cancers and correlates with activating mutations in K-Ras
Eunice Laurent
Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, GA 30322, USA
Int J Cancer 123:100-7. 2008
..We conclude that Nox1 mRNA and protein are overexpressed in colon cancer and are strongly correlated with activating mutations in K-Ras...
- Upregulation of Nox1 in vascular smooth muscle leads to impaired endothelium-dependent relaxation via eNOS uncoupling
Anna E Dikalova
Department of Medicine, Emory University, Atlanta, Georgia 30322, USA
Am J Physiol Heart Circ Physiol 299:H673-9. 2010
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- Spring brings breezes, wheezes, and pollen oxidases
Darren R Ritsick
Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, Georgia 30322, USA
J Clin Invest 115:2067-9. 2005
..These findings suggest that inhibition of the pollen oxidase may provide a way to antagonize allergic inflammation at a very early step...
- Reactive oxygen generated by Nox1 triggers the angiogenic switch
Jack L Arbiser
Department of Dermatology, Emory University School of Medicine, Atlanta, GA 30322, USA
Proc Natl Acad Sci U S A 99:715-20. 2002
..Nox1 induction of VEGF is eliminated by coexpression of catalase, indicating that hydrogen peroxide signals part of the switch to the angiogenic phenotype...
- Nox1 expression determines cellular reactive oxygen and modulates c-fos-induced growth factor, interleukin-8, and Cav-1
Rebecca S Arnold
Department of Urology, Winship Cancer Institute, Emory University School of Medicine, 1365 Clifton Rd, Building B, Atlanta, GA 30322, USA
Am J Pathol 171:2021-32. 2007
..0001). These studies indicate that Nox1 overexpression may function as a reversible signal for cellular proliferation with relevance for a common human tumor...
- Redox-dependent expression of cyclin D1 and cell proliferation by Nox1 in mouse lung epithelial cells
Priya Ranjan
Department of Pathology and Vermont Cancer Center, University of Vermont College of Medicine, Burlington, 05405, USA
Antioxid Redox Signal 8:1447-59. 2006
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- Creation of a genetic system for analysis of the phagocyte respiratory burst: high-level reconstitution of the NADPH oxidase in a nonhematopoietic system
Marianne O Price
Herman B Wells Center for Pediatric Research, Department of Pediatrics, James Whitcomb Riley Hospital for Children, Indiana University Medical Center, Indianapolis 46202, USA
Blood 99:2653-61. 2002
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- Contrasting roles of NADPH oxidase isoforms in pressure-overload versus angiotensin II-induced cardiac hypertrophy
Jonathan A Byrne
Department of Cardiology, King s College London, London, UK
Circ Res 93:802-5. 2003
..These data suggest a differential response of the cardiac Nox isoforms, gp91phox and Nox4, to Ang II versus pressure overload...
- NOX5 NAD(P)H oxidase regulates growth and apoptosis in DU 145 prostate cancer cells
Sukhdev S Brar
Department of Internal Medicine, Carolinas Medical Center, Charlotte, NC 28232, USA
Am J Physiol Cell Physiol 285:C353-69. 2003
..These results indicate that ROS generated by the newly described NOX5 oxidase are essential for prostate cancer growth, possibly by providing trophic intracellular oxidant tone that retards programmed cell death...
- The NAD(P)H oxidase homolog Nox4 modulates insulin-stimulated generation of H2O2 and plays an integral role in insulin signal transduction
Kalyankar Mahadev
Dorrance H Hamilton Research Laboratories, Division of Endocrinology, Diabetes and Metabolic Diseases, Department of Medicine, Jefferson Medical College, Thomas Jefferson University, Philadelphia, Pennsylvania 19107 6799, USA
Mol Cell Biol 24:1844-54. 2004
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- The superoxide-generating oxidase Nox1 is functionally required for Ras oncogene transformation
Junji Mitsushita
Department of Molecular Biology and Biochemistry, Shinshu University School of Medicine, Matsumoto, Nagano, Japan
Cancer Res 64:3580-5. 2004
..Therefore, we propose that increased reactive oxygen species generation by Ras-induced Nox1 is required for oncogenic Ras transformation...
