Developmental signaling: shrimp and strawberries help flies make conesEric C Lai
545 Life Sciences Addition, University of California, Department of Molecular and Cell Biology, Berkeley, CA 947200 3200, USA
Curr Biol 12:R722-4. 2002
..A new study has revealed a serial linkage between them, via Ebi and Strawberry Notch, which is important in determining the cone cell fate in the Drosophila eye...
Pervasive regulation of Drosophila Notch target genes by GY-box-, Brd-box-, and K-box-class microRNAsEric C Lai
Department of Molecular and Cell Biology Howard Hughes Medical Institute, University of California at Berkeley, Berkeley, CA 94720 3200, USA
Genes Dev 19:1067-80. 2005
..Collectively, these data establish insights into miRNA target recognition and demonstrate that the Notch signaling pathway is a major target of miRNA-mediated regulation in Drosophila...
- A hidden program in Drosophila peripheral neurogenesis revealed: fundamental principles underlying sensory organ diversity
Eric C Lai
Howard Hughes Medical Institute, 545 Life Sciences Addition, Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720 3200, USA
Dev Biol 269:1-17. 2004
..We propose that most Drosophila sensory organs are built from an archetypal lineage, and we speculate about how this stereotyped pattern of cell divisions may have been built during evolution...
- The ubiquitin ligase Drosophila Mind bomb promotes Notch signaling by regulating the localization and activity of Serrate and Delta
Eric C Lai
Department of Molecular and Cell Biology, Howard Hughes Medical Institute, 545 Life Sciences Addition, University of California at Berkeley, Berkeley, CA 94720 3200, USA
Development 132:2319-32. 2005
..We conclude that ubiquitination of Delta and Serrate by Neuralized and D-mib is an obligate feature of DSL ligand activation throughout Drosophila development...
- Notch signaling: control of cell communication and cell fate
Eric C Lai
Howard Hughes Medical Institute, 545 Life Sciences Addition, Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720 3200, USA
Development 131:965-73. 2004
..This primer describes the mechanism of Notch signal transduction and how it is used to control the formation of biological patterns...
- The Drosophila microRNA iab-4 causes a dominant homeotic transformation of halteres to wings
Matthew Ronshaugen
Department of Molecular and Cell Biology, Division of Genetics, Center for Integrative Genomics, University of California, Berkeley, 94720, USA
Genes Dev 19:2947-52. 2005
..These findings provide the first evidence for a noncoding homeotic gene and raise the possibility that other such genes occur within the Bithorax complex. We also discuss the regulation of mir-iab-4 expression during development...
- Hybrid neurons in a microRNA mutant are putative evolutionary intermediates in insect CO2 sensory systems
Pelin Cayirlioglu
Department of Biological Chemistry, Howard Hughes Medical Institute, David Geffen School of Medicine, University of California, Los Angeles, CA 90095, USA
Science 319:1256-60. 2008
..We propose that this hybrid ORN reveals a cellular intermediate in the evolution of species-specific behaviors elicited by CO2...
- miRNAs: whys and wherefores of miRNA-mediated regulation
Eric C Lai
Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA
Curr Biol 15:R458-60. 2005
..New bioinformatic and genetic studies are setting the stage for unraveling the specific biological functions of miRNAs...
- Drosophila microRNAs exhibit diverse spatial expression patterns during embryonic development
A Aziz Aboobaker
Department of Molecular and Cell Biology, University of California, Berkeley, 94720 3200, USA
Proc Natl Acad Sci U S A 102:18017-22. 2005
..Intriguingly, the expression patterns of several fly miRNAs are analogous to those of their vertebrate counterparts, suggesting that these miRNAs may have ancient roles in animal patterning...
- Complementary miRNA pairs suggest a regulatory role for miRNA:miRNA duplexes
Eric C Lai
Howard Hughes Medical Institute, University of California, Berkeley, Department of Molecular and Cell Biology, Berkeley, California 94720 3200, USA
RNA 10:171-5. 2004
....
- Cross GTPase-activating protein (CrossGAP)/Vilse links the Roundabout receptor to Rac to regulate midline repulsion
Hailan Hu
Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA
Proc Natl Acad Sci U S A 102:4613-8. 2005
..Dosage-sensitive genetic interactions among CrGAP, Robo, and Rac support a model in which CrGAP transduces signals downstream of Robo receptor to regulate Rac-dependent cytoskeletal changes...
- Drosophila tufted is a gain-of-function allele of the proneural gene amos
Eric C Lai
Howard Hughes Medical Institute, Department of Molecular and Cell Biology, University of California, Berkeley, California 94720, USA
Genetics 163:1413-25. 2003
..This phenomenon seems instead to be related to their shared ability to activate Asense and Senseless...
- Virus discovery by deep sequencing and assembly of virus-derived small silencing RNAs
Qingfa Wu
Department of Plant Pathology and Microbiology, Institute for Integrative Genome Biology, University of California, Riverside, CA 92521, USA
Proc Natl Acad Sci U S A 107:1606-11. 2010
..We propose that invertebrate viruses discovered by this approach may include previously undescribed human and vertebrate viral pathogens that are transmitted by arthropod vectors...
- microRNAs: runts of the genome assert themselves
Eric C Lai
Department of Molecular and Cell Biology, University of California/HHMI, 645 Life Sciences Addition, Berkeley, CA 94720-3200, USA
Curr Biol 13:R925-36. 2003
- RNA sensors and riboswitches: self-regulating messages
Eric C Lai
545 Life Sciences Addition, University of California, Department of Molecular and Cell Biology, Berkeley, CA 94720 3200, USA
Curr Biol 13:R285-91. 2003
..Remarkably, these sensors allow associated mRNAs to regulate their own transcription or translation accordingly, without the need for regulatory proteins...
- Keeping a good pathway down: transcriptional repression of Notch pathway target genes by CSL proteins
Eric C Lai
Department of Molecular and Cell Biology, University of California, Berkeley, 94720 3200, USA
EMBO Rep 3:840-5. 2002
..This review highlights differences in composition and similarities in function of different CSL co-repressor complexes, which actively repress Notch pathway target genes in the absence of Notch pathway activity...
Predicting and validating microRNA targetsEric C Lai
545 Life Sciences Addition, Department of Molecular and Cell Biology and Howard Hughes Medical Institute, University of California at Berkeley, Berkeley, CA 94720 3200, USA
Genome Biol 5:115. 2004
..There has been considerable progress, but assessing success and biological significance requires a move into the 'wet' lab...
- Computational identification of Drosophila microRNA genes
Eric C Lai
Howard Hughes Medical Institute, Department of Molecular and Cell Biology, University of California at Berkeley, 539 Life Sciences Addition, Berkeley, CA 94720, USA
Genome Biol 4:R42. 2003
..Second, miRNAs are usually highly conserved between the genomes of related species. Third, miRNAs display a characteristic pattern of evolutionary divergence...
- Micro RNAs are complementary to 3' UTR sequence motifs that mediate negative post-transcriptional regulation
Eric C Lai
University of California at Berkeley, Department of Molecular and Cell Biology, 545 Life Sciences Addition 3200, Berkeley, California 94720 3200, USA
Nat Genet 30:363-4. 2002
..These findings suggest a more general role for micro RNAs in gene regulation through the formation of RNA duplexes...
- Protein degradation: four E3s for the notch pathway
Eric C Lai
University of California, Department of Molecular and Cell Biology, Berkeley, California 94720 3200, USA
Curr Biol 12:R74-8. 2002
..These include Suppressor of deltex/Itch and Sel-10, which both regulate Notch, Neuralized, which regulates the Notch ligand Delta, and LNX, which regulates the Notch antagonist Numb...
- Notch cleavage: Nicastrin helps Presenilin make the final cut
Eric C Lai
University of California, Department of Molecular and Cell Biology, Berkeley 94720 3200, USA
Curr Biol 12:R200-2. 2002
..Recent studies have identified the novel protein Nicastrin as another essential component of the Presenilin/gamma-secretase complex...
- The mirtron pathway generates microRNA-class regulatory RNAs in Drosophila
Katsutomo Okamura
Memorial Sloan Kettering Cancer Center, Department of Developmental Biology, 1275 York Ave, Box 252, New York, NY 10021, USA
Cell 130:89-100. 2007
..These findings reveal that mirtrons are an alternate source of miRNA-type regulatory RNAs...
- Discovery of functional elements in 12 Drosophila genomes using evolutionary signatures
Alexander Stark
The Broad Institute, Massachusetts Institute of Technology and Harvard University, Cambridge, Massachusetts 02140, USA
Nature 450:219-32. 2007
..We also study how discovery power scales with the divergence and number of species compared, and we provide general guidelines for comparative studies...
- Functionally distinct regulatory RNAs generated by bidirectional transcription and processing of microRNA loci
David M Tyler
Department of Developmental Biology, Sloan Kettering Institute, New York, New York 10021, USA
Genes Dev 22:26-36. 2008
..These findings demonstrate that antisense transcription and processing contributes to the functional diversification of miRNA genes...
- The Drosophila hairpin RNA pathway generates endogenous short interfering RNAs
Katsutomo Okamura
Sloan Kettering Institute, Department of Developmental Biology, 521 Rockefeller Research Laboratories, 1275 York Avenue, Box 252, New York, New York 10065, USA
Nature 453:803-6. 2008
..These novel regulatory RNAs reveal unexpected complexity in the sorting of small RNAs, and open a window onto the biological usage of endogenous RNA interference in Drosophila...
- Two distinct mechanisms generate endogenous siRNAs from bidirectional transcription in Drosophila melanogaster
Katsutomo Okamura
Sloan Kettering Institute, Department of Developmental Biology, 521 Rockefeller Research Laboratories, 1275 York Avenue, Box 252, New York, New York 10065, USA
Nat Struct Mol Biol 15:581-90. 2008
..These newly recognized siRNA pathways broaden the scope of regulatory networks mediated by small RNAs...
- Endogenous RNA interference provides a somatic defense against Drosophila transposons
Wei Jen Chung
Department of Developmental Biology, Sloan Kettering Institute, 521 Rockefeller Research Laboratories, 1275 York Ave, Box 252, New York, New York 10065, USA
Curr Biol 18:795-802. 2008
..The piRNA system is thought to provide primarily a germline defense against TE activity...
- Functional screening identifies miR-315 as a potent activator of Wingless signaling
Serena J Silver
Department of Genetics, Howard Hughes Medical Institute, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA 02115, USA
Proc Natl Acad Sci U S A 104:18151-6. 2007
..In summary, activity-based screening can selectively identify miRNAs whose deregulation can lead to interpretable phenotypic consequences...
