Zsombor Lacza

Summary

Publications

  1. ncbi request reprint Mitochondria produce reactive nitrogen species via an arginine-independent pathway
    Zsombor Lacza
    Department of Physiology Pharmacology, Wake Forest University Health Sciences, Winston Salem, NC 27157, USA
    Free Radic Res 40:369-78. 2006
  2. ncbi request reprint Neural stem cell transplantation in cold lesion: a novel approach for the investigation of brain trauma and repair
    Zsombor Lacza
    Department of Physiology Pharmacology, Wake Forest University School of Medicine, Medical Center Blvd, Winston Salem, NC 27157, USA
    Brain Res Brain Res Protoc 11:145-54. 2003
  3. ncbi request reprint Urotensin-II is a nitric oxide-dependent vasodilator in the pial arteries of the newborn pig
    Zsombor Lacza
    Department of Physiology Pharmacology, Wake Forest University Health Sciences, Medical Center Blvd, Winston Salem, NC 27157, USA
    Life Sci 78:2763-6. 2006
  4. ncbi request reprint The novel red-fluorescent probe DAR-4M measures reactive nitrogen species rather than NO
    Zsombor Lacza
    Department of Physiology Pharmacology, Wake Forest University School of Medicine, Medical Center Blvd, Winston Salem, NC 27157, USA
    J Pharmacol Toxicol Methods 52:335-40. 2005
  5. ncbi request reprint Mitochondrial NO and reactive nitrogen species production: does mtNOS exist?
    Zsombor Lacza
    Institute of Human Physiology and Clinical Experimental Research, Semmelweis University, Üllői út 78 a, 1082 Budapest, Hungary
    Nitric Oxide 14:162-8. 2006
  6. ncbi request reprint Lack of mitochondrial nitric oxide production in the mouse brain
    Zsombor Lacza
    Department of Physiology Pharmacology, Wake Forest University School of Medicine, Winston Salem, North Carolina, USA
    J Neurochem 90:942-51. 2004
  7. ncbi request reprint Investigation of the subunit composition and the pharmacology of the mitochondrial ATP-dependent K+ channel in the brain
    Zsombor Lacza
    Department of Physiology Pharmacology, Wake Forest University School of Medicine, Winston Salem, NC 27157, USA
    Brain Res 994:27-36. 2003
  8. ncbi request reprint Mitochondrial nitric oxide synthase is not eNOS, nNOS or iNOS
    Zsombor Lacza
    Department of Physiology Pharmacology, Wake Forest University School of Medicine, Medical Center Blvd, Winston Salem, NC, USA
    Free Radic Biol Med 35:1217-28. 2003
  9. ncbi request reprint Heart mitochondria contain functional ATP-dependent K+ channels
    Zsombor Lacza
    Department of Physiology and Pharmacology, Wake Forest University School of Medicine, Winston Salem, NC 27157, USA
    J Mol Cell Cardiol 35:1339-47. 2003
  10. ncbi request reprint PARP inhibition improves the effectiveness of neural stem cell transplantation in experimental brain trauma
    Zsombor Lacza
    Department of Physioloty Pharmacology, Wake Forest University School of Medicine, Winston Salem, NC 27157, USA
    Int J Mol Med 12:153-9. 2003

Collaborators

Detail Information

Publications41

  1. ncbi request reprint Mitochondria produce reactive nitrogen species via an arginine-independent pathway
    Zsombor Lacza
    Department of Physiology Pharmacology, Wake Forest University Health Sciences, Winston Salem, NC 27157, USA
    Free Radic Res 40:369-78. 2006
    ..We conclude that mitochondria from brain, heart and liver are capable of significant RNS production via the respiratory chain rather than through an arginine-dependent mtNOS...
  2. ncbi request reprint Neural stem cell transplantation in cold lesion: a novel approach for the investigation of brain trauma and repair
    Zsombor Lacza
    Department of Physiology Pharmacology, Wake Forest University School of Medicine, Medical Center Blvd, Winston Salem, NC 27157, USA
    Brain Res Brain Res Protoc 11:145-54. 2003
    ..The model is a combination of surgical, neurological and histological approaches, all adapted to each other to make a reliable and reproducible experimental model...
  3. ncbi request reprint Urotensin-II is a nitric oxide-dependent vasodilator in the pial arteries of the newborn pig
    Zsombor Lacza
    Department of Physiology Pharmacology, Wake Forest University Health Sciences, Medical Center Blvd, Winston Salem, NC 27157, USA
    Life Sci 78:2763-6. 2006
    ..We conclude that UT-II, in contrast to most other vascular beds, is a weak NO-dependent vasodilator in the piglet pial vasculature...
  4. ncbi request reprint The novel red-fluorescent probe DAR-4M measures reactive nitrogen species rather than NO
    Zsombor Lacza
    Department of Physiology Pharmacology, Wake Forest University School of Medicine, Medical Center Blvd, Winston Salem, NC 27157, USA
    J Pharmacol Toxicol Methods 52:335-40. 2005
    ..In the present study we tested the reaction of a novel rhodamine-based chromophore diaminorhodamine-4M (DAR-4M) with NO and other reactive nitrogen and oxygen species...
  5. ncbi request reprint Mitochondrial NO and reactive nitrogen species production: does mtNOS exist?
    Zsombor Lacza
    Institute of Human Physiology and Clinical Experimental Research, Semmelweis University, Üllői út 78 a, 1082 Budapest, Hungary
    Nitric Oxide 14:162-8. 2006
    ..The present review aims to summarize the most recent developments in mitochondrial NO production, highlights a few unsolved questions, and proposes new directions for future work in this research area...
  6. ncbi request reprint Lack of mitochondrial nitric oxide production in the mouse brain
    Zsombor Lacza
    Department of Physiology Pharmacology, Wake Forest University School of Medicine, Winston Salem, North Carolina, USA
    J Neurochem 90:942-51. 2004
    ..We conclude that mouse brain mitochondria do not contain NOS isoforms, nor do they produce NO through a NOS-dependent mechanism...
  7. ncbi request reprint Investigation of the subunit composition and the pharmacology of the mitochondrial ATP-dependent K+ channel in the brain
    Zsombor Lacza
    Department of Physiology Pharmacology, Wake Forest University School of Medicine, Winston Salem, NC 27157, USA
    Brain Res 994:27-36. 2003
    ..We conclude that brain mitochondria contain functional K(ATP) channels. The pore-forming subunit of the channel can be either Kir6.1 or Kir6.2, and the SUR subunit may be a SUR2 splice variant or a similar protein...
  8. ncbi request reprint Mitochondrial nitric oxide synthase is not eNOS, nNOS or iNOS
    Zsombor Lacza
    Department of Physiology Pharmacology, Wake Forest University School of Medicine, Medical Center Blvd, Winston Salem, NC, USA
    Free Radic Biol Med 35:1217-28. 2003
    ..Taken together, the presented data do not support the hypothesis that any of the known NOS enzymes are present in the mitochondria in physiologically relevant levels...
  9. ncbi request reprint Heart mitochondria contain functional ATP-dependent K+ channels
    Zsombor Lacza
    Department of Physiology and Pharmacology, Wake Forest University School of Medicine, Winston Salem, NC 27157, USA
    J Mol Cell Cardiol 35:1339-47. 2003
    ..We conclude that a functional K(ATP) channel is present in heart mitochondria, which can be opened by diazoxide or BMS-191095. The channel can be composed of Kir6.1 and Kir6.2 subunits and does not contain either SUR1 or SUR2...
  10. ncbi request reprint PARP inhibition improves the effectiveness of neural stem cell transplantation in experimental brain trauma
    Zsombor Lacza
    Department of Physioloty Pharmacology, Wake Forest University School of Medicine, Winston Salem, NC 27157, USA
    Int J Mol Med 12:153-9. 2003
    ..Inhibition of the ONOO- - PARP activation cascade significantly improves the effectiveness of neural stem cell transplantation and promotes rapid functional recovery...
  11. ncbi request reprint Prostacyclin-mediated compensatory mechanism in the coronary circulation during acute NO synthase blockade
    Zsombor Lacza
    Institute of Human Physiology and Clinical Experimental Research, Semmelweis University, Üllői út 78 a, H 1082 Budapest, Hungary
    Life Sci 73:1141-9. 2003
    ..We conclude that in case of decreased NO levels in the coronary circulation, arterial tone is maintained by prostacyclin production...
  12. ncbi request reprint Additive effect of cyclooxygenase and nitric oxide synthase blockade on the cerebrocortical microcirculation
    Zsombor Lacza
    Institute of Human Physiology and Clinical Experimental Research, Semmelweis University, Budapest, Hungary
    Neuroreport 20:1027-31. 2009
    ..Therefore, the COX pathway seems to play a similar role under physiological and NO-deficient conditions in the maintenance of the resting CBF...
  13. ncbi request reprint Graft derived cells with double nuclei in the penumbral region of experimental brain trauma
    Eszter M Horvath
    Department of Physiology and Pharmacology, Wake Forest University Health Sciences, Winston Salem, NC, USA
    Neurosci Lett 396:182-6. 2006
    ..Thus, these cells showed a possible sign of cell fusion in the penumbral region of the injured brain...
  14. ncbi request reprint Mechanisms of pain-induced local cerebral blood flow changes in the rat sensory cortex and thalamus
    Benedek Erdos
    Institute of Human Physiology and Clinical Experimental Research, Semmelweis University, Budapest, Hungary
    Brain Res 960:219-27. 2003
    ....
  15. ncbi request reprint Somatosensory pain does not affect total cerebral blood volume
    Tamas Mersich
    Institute of Human Physiology and Clinical Experimental Research, Semmelweis University Faculty of Medicine, Budapest, Hungary
    Neuroreport 18:649-52. 2007
    ..from 5.0+/-0.6 to 4.24+/-0.9 vol%). Nitric oxide synthase blockade, however, did not affect either the stimulation induced increase of regional cerebral blood flow or the steadiness of total cerebral blood volume during the stimulation...
  16. ncbi request reprint Targeting mitochondrial ATP-sensitive potassium channels--a novel approach to neuroprotection
    David W Busija
    Department of Physiology and Pharmacology, and Center for Investigative Neuroscience, Wake Forest University Health Sciences, Medical Center Boulevard, Winston Salem, NC 27157 1010, USA
    Brain Res Brain Res Rev 46:282-94. 2004
    ..However, little is known about the mechanisms involved. The purpose of this review is to detail the current state of knowledge about this important, emerging area of investigation, and to provide suggestions for future studies...
  17. ncbi request reprint MitoK(ATP) opener, diazoxide, reduces neuronal damage after middle cerebral artery occlusion in the rat
    Katsuyoshi Shimizu
    Department of Physiology and Pharmacology, Wake Forest University School of Medicine, Winston Salem, North Carolina 27157 1083, USA
    Am J Physiol Heart Circ Physiol 283:H1005-11. 2002
    ..These results indicate that selective opening of the mitoK(ATP) channel has neuroprotective effects against ischemia-reperfusion injury in the rat brain...
  18. ncbi request reprint Piglet pial arteries respond to N-methyl-D-aspartate in vivo but not in vitro
    Steve A Simandle
    Department of Pediatrics, Division of Neonatology, Wake Forest University School of Medicine, Medical Center Boulevard, Winston Salem, NC 27157 1010, USA
    Microvasc Res 70:76-83. 2005
    ..This work confirms our previous in vivo data and is consistent with the hypothesis that cerebral arteries respond to NMDA through a secondary interaction mediated by neuronal release of NO and not to NMDA directly...
  19. ncbi request reprint Hydrogen peroxide acts as an EDHF in the piglet pial vasculature in response to bradykinin
    Zsombor Lacza
    Department of Physiology and Pharmacology, Wake Forest University School of Medicine, Winston Salem, North Carolina 27157, USA
    Am J Physiol Heart Circ Physiol 283:H406-11. 2002
    ..We conclude that H(2)O(2) mediates the non-nitric oxide-, non-prostanoid-dependent vasorelaxation to BK in the piglet pial vasculature. The response is mediated via BK(2) receptors and the opening of K(ATP) channels...
  20. doi request reprint Albumin-coated bioactive suture for cell transplantation
    Dénes Balázs Horváthy
    Institute of Human Physiology and Clinical Experimental Research, Semmelweis University, Budapest, Hungary
    Surg Innov 20:249-55. 2013
    ..Using these stitches in vivo resulted in short-term and long-term survival of cells. As a result, albumin-coated suture can be a vehicle for stem cell therapy in soft tissues such as muscle, tendon, or peripheral nerves...
  21. pmc Serum albumin enhances bone healing in a nonunion femoral defect model in rats: a computer tomography micromorphometry study
    Gábor Skaliczki
    Department of Orthopaedics, Semmelweis University, Karolina ut 27, 1113, Budapest, Hungary
    Int Orthop 37:741-5. 2013
    ..Our earlier studies showed that serum albumin itself can markedly enhance the proliferation of stem cells on bone allograft and postulated that albumin coating alone may improve bone graft integration in vivo...
  22. ncbi request reprint Activation of mitochondrial ATP-sensitive potassium channels prevents neuronal cell death after ischemia in neonatal rats
    Nishadi Rajapakse
    Department of Physiology and Pharmacology, Wake Forest University School of Medicine, Medical Center Boulevard, Winston Salem, NC 27157, USA
    Neurosci Lett 327:208-12. 2002
    ..Fluorescent studies indicated DIZ directly depolarized the mitochondria. In conclusion, selective opening of mK(ATP) prior to HI results in neuroprotection in immature rats...
  23. pmc Mesenchymal stem cells rescue cardiomyoblasts from cell death in an in vitro ischemia model via direct cell-to-cell connections
    Attila Cselenyák
    Institute of Human Physiology and Clinical Experimental Research, Semmelweis University, H 1094, Tuzoltó utca 37 47, Budapest, Hungary
    BMC Cell Biol 11:29. 2010
    ..Using an in vitro ischemia model of 150 minutes of oxygen glucose deprivation we investigated cell viability and cell interactions with confocal microscopy and flow cytometry...
  24. pmc Human internal thoracic artery grafts exhibit severe morphological and functional damage and spasmic vasomotion due to oxidative stress
    Levente Kiss
    Institute of Human Physiology and Clinical Experimental Research, Semmelweis University, Budapest, Hungary
    Med Sci Monit 17:CR411-6. 2011
    ..We aimed to investigate and describe the histology, PARP activation and functionality of ITA grafts and to assess the possible beneficial effect of PARP-inhibition...
  25. pmc Role of endocannabinoids and cannabinoid-1 receptors in cerebrocortical blood flow regulation
    András Iring
    Institute of Human Physiology and Clinical Experimental Research, Semmelweis University, Budapest, Hungary
    PLoS ONE 8:e53390. 2013
    ....
  26. pmc Hypersensitivity to thromboxane receptor mediated cerebral vasomotion and CBF oscillations during acute NO-deficiency in rats
    Bėla Horváth
    Institute of Human Physiology and Clinical Experimental Research, Semmelweis University, Budapest, Hungary
    PLoS ONE 5:e14477. 2010
    ..In the present study we hypothesized that in the absence of NO, overactivation of the TP-receptor mediated cerebrovascular signaling pathway contributes to the development of vasomotion and CBF oscillations...
  27. ncbi request reprint Human heart mitochondria do not produce physiologically relevant quantities of nitric oxide
    Attila Csordas
    Department of Human Physiology and Clinical Experimental Research, Semmelweis University, Budapest, Hungary
    Life Sci 80:633-7. 2007
    ..These results indicate that mitochondria do not produce physiologically relevant quantities of NO in the heart and are unlikely to have any physiological importance as NO donors, nor do they contain a recognizable mtNOS enzyme...
  28. doi request reprint The role of mitochondria in direct cell-to-cell connection dependent rescue of postischemic cardiomyoblasts
    Eszter Pankotai
    Department of Human Physiology and Clinical Experimental Research, Semmelweis University, Budapest, Hungary
    Mitochondrion 12:352-6. 2012
    ..This novel mechanism opens a new possiblity for cell-based cardiac repair in ischemic heart disease...
  29. ncbi request reprint Mitochondrial nitric oxide synthase: current concepts and controversies
    Zsombor Lacza
    Department of Human Physiology and Clinical Experimental Research, Semmelweis University, 1082 Budapest, Hungary
    Front Biosci (Landmark Ed) 14:4436-43. 2009
    ..This review summarizes the experimental evidence gathered over the last decade in this field and highlights new areas of research that reveal surprising dimensions of NO production and metabolism by mitochondria...
  30. ncbi request reprint Effects of 7-ketocholesterol on the activity of endothelial poly(ADP-ribose) polymerase and on endothelium-dependent relaxant function
    Levente Kiss
    Department of Human Physiology and Clinical Experimental Research, Semmelweis University, Budapest 1082, Hungary
    Int J Mol Med 18:1113-7. 2006
    ..Our experimental results suggest that although 7-ketocholesterol can activate PARP enzyme in endothelial cells, it is not sufficient on its own to cause impairment in the endothelium-dependent vascular reactivity...
  31. ncbi request reprint Freeze-dried human serum albumin improves the adherence and proliferation of mesenchymal stem cells on mineralized human bone allografts
    Miklós Weszl
    Tissue Engineering Laboratory, Department of Human Physiology and Clinical Experimental Research, Semmelweis University, 1094 Budapest Tu zolt utcaó 37 47, Budapest 1094, Hungary
    J Orthop Res 30:489-96. 2012
    ..Micro-hardness measurements confirmed that albumin coating does not influence the physical characteristics of the scaffold, so it is possible to introduce albumin coating into the manufacturing process of lyophilized bone allografts...
  32. ncbi request reprint Current developments in the therapeutic potential of S-nitrosoglutathione, an endogenous NO-donor molecule
    István Hornyák
    Semmelweis University, Department of Human Physiology and Clinical Experimental Research, H 1094, Budapest, Tuzolto Street 37 47, Hungary
    Curr Pharm Biotechnol 12:1368-74. 2011
    ..The review focuses on the chemical and biological data which support the therapeutic use of GSNO and highlights areas where further research is needed...
  33. doi request reprint Increased stability of S-nitrosothiol solutions via pH modulations
    István Hornyák
    Semmelweis University, Department of Human Physiology and Clinical Experimental Research, Budapest, Hungary
    Free Radic Res 46:214-25. 2012
    ..8% of the initial GSNO concentration for 25 days. This amount of topically administered GSNO was still capable of increasing the dermal blood flow over 200% in human subjects...
  34. doi request reprint Intra-mitochondrial poly(ADP-ribosyl)ation: potential role for alpha-ketoglutarate dehydrogenase
    Eszter Pankotai
    Department of Human Physiology and Clinical Experimental Research, Semmelweis University, Budapest, Hungary
    Mitochondrion 9:159-64. 2009
    ..The KGDH enzyme may, therefore, have a novel function as a PARP-like enzyme, which may play a role in regulating intramitochondrial NAD(+) and poly(ADP-ribose) homeostasis, with possible roles in physiology and pathophysiology...
  35. ncbi request reprint Integration of neuronally predifferentiated human dental pulp stem cells into rat brain in vivo
    Marianna Király
    Department of Oral Biology, Semmelweis University, Budapest, Hungary
    Neurochem Int 59:371-81. 2011
    ..This proof of concept study reveals that predifferentiated hDPSCs may serve as useful sources of neuro- and gliogenesis in vivo, especially when the brain is injured...
  36. pmc Combined exercise and insulin-like growth factor-1 supplementation induces neurogenesis in old rats, but do not attenuate age-associated DNA damage
    Erika Koltai
    Semmelweis University, Research Institute of Sport Science, Budapest, Hungary
    Rejuvenation Res 14:585-96. 2011
    ..Age-associated decreases in SIRT1 and the associated increase in lysine acetylation, in the hippocampus, could have significant impact on function and thus, could suggest a therapeutic target...
  37. ncbi request reprint [Influence of diabetes mellitus on cerebral ischemia and reperfusion injury]
    Marianna Muranyi
    Semmelweis Egyetem, Általános Orvostudományi Kar, Klinikai Kísérleti Kutató és Humán Elettani Intézet, Budapest
    Orv Hetil 147:1885-9. 2006
    ..In summary, diabetes mellitus enhances intracellular pathways activated by cerebral ischemia and leads to exaggerated brain damage in diabetic subjects...
  38. pmc Activation of poly(ADP-ribose) polymerase by myocardial ischemia and coronary reperfusion in human circulating leukocytes
    Emese Toth-Zsamboki
    Cardiovascular Research Group of the Hungarian Academy of Sciences and Semmelweis University and National Health Center, Budapest, Hungary
    Mol Med 12:221-8. 2006
    ..These results represent the first direct demonstration of PARP activation in human myocardial infarction. Future work is required to test whether pharmacological inhibition of PARP may offer myocardial protection during primary PCI...
  39. ncbi request reprint [Astrocytes in health and disease]
    Marianna Muranyi
    Semmelweis Egyetem, Általános Orvostudományi Kar, Klinikai Kísérleti Kutató és Humán Elettani Intézet, Budapest
    Orv Hetil 148:697-702. 2007
    ..Thus, investigation of the molecular mechanisms of astrocyte function may reveal novel targets for the development of therapeutic tools in neuronal diseases...
  40. ncbi request reprint [Neuroprotection in brain ischemia--doubts and hopes]
    Zsolt Zador
    Szegedi Tudomanyegyetem, Patológiai Intézet, H 6701 Szeged
    Ideggyogy Sz 57:81-93. 2004
    ..Strategies to facilitate brain plasticity and regeneration is an additional promising tool to enhance recovery in brain ischaemia...