B Laber

Summary

Publications

  1. ncbi Characterization of recombinant Arabidopsis thaliana threonine synthase
    B Laber
    Hoechst Schering AgrEvo GmbH, Biochemical Research, Frankfurt am Main, Germany
    Eur J Biochem 263:212-21. 1999
  2. ncbi Vitamin B6 biosynthesis: formation of pyridoxine 5'-phosphate from 4-(phosphohydroxy)-L-threonine and 1-deoxy-D-xylulose-5-phosphate by PdxA and PdxJ protein
    B Laber
    Hoechst Schering AgrEvo GmbH, Biochemical Research, Frankfurt am Main, Germany
    FEBS Lett 449:45-8. 1999
  3. ncbi Cloning, purification, and crystallization of Escherichia coli cystathionine beta-lyase
    B Laber
    Hoechst Schering AgrEvo GmbH, Berlin, Germany
    FEBS Lett 379:94-6. 1996
  4. ncbi Structural basis for the function of pyridoxine 5'-phosphate synthase
    M G Franco
    Max Planck Institut fur Biochemie, Abteilung Strukturforschung, D 82152 Planegg Martinsried, Germany
    Structure 9:245-53. 2001
  5. ncbi Crystallization and preliminary X-ray crystallographic analysis of PdxJ, the pyridoxine 5'-phosphate synthesizing enzyme
    M Garrido Franco
    Max Planck Institute of Biochemistry, Department of Structural Investigation, Am Klopferspitz 18a, D 82152 Martinsried, Germany
    Acta Crystallogr D Biol Crystallogr 56:1045-8. 2000
  6. ncbi Slow-binding inhibition of Escherichia coli cystathionine beta-lyase by L-aminoethoxyvinylglycine: a kinetic and X-ray study
    T Clausen
    Max Planck Institut fur Biochemie, Abteilung Strukturforschung, Am Klopferspitz 18a, D 82152 Martinsried, Germany
    Biochemistry 36:12633-43. 1997
  7. ncbi Mode of action of cystathionine beta-lyase
    T Clausen
    Max Planck Institut fur Biochemie, Abteilung Strukturforschung, Martinsried, Germany
    Biol Chem 378:321-6. 1997
  8. ncbi Reaction mechanism of Escherichia coli dihydrodipicolinate synthase investigated by X-ray crystallography and NMR spectroscopy
    S Blickling
    Max Planck Institut fur Biochemie, Abteilung Strukturforschung, Martinsried, FRG
    Biochemistry 36:24-33. 1997
  9. ncbi Crystal structure of the pyridoxal-5'-phosphate dependent cystathionine beta-lyase from Escherichia coli at 1.83 A
    T Clausen
    Max Planck Institut fur Biochemie, Abteilung Strukturforschung, Martinsried Germany
    J Mol Biol 262:202-24. 1996
  10. pmc Escherichia coli dihydrodipicolinate synthase. Identification of the active site and crystallization
    B Laber
    Max Planck Institute für Biochemie, Martinsried, Federal Republic of Germany
    Biochem J 288:691-5. 1992

Collaborators

Detail Information

Publications10

  1. ncbi Characterization of recombinant Arabidopsis thaliana threonine synthase
    B Laber
    Hoechst Schering AgrEvo GmbH, Biochemical Research, Frankfurt am Main, Germany
    Eur J Biochem 263:212-21. 1999
    ..The crystals diffracted to beyond 0.28 nm resolution and belonged to the space group P222 (unit cell parameters: a = 6.16 nm, b = 10.54 nm, c = 14.63 nm, alpha = beta = gamma = 90 degrees)...
  2. ncbi Vitamin B6 biosynthesis: formation of pyridoxine 5'-phosphate from 4-(phosphohydroxy)-L-threonine and 1-deoxy-D-xylulose-5-phosphate by PdxA and PdxJ protein
    B Laber
    Hoechst Schering AgrEvo GmbH, Biochemical Research, Frankfurt am Main, Germany
    FEBS Lett 449:45-8. 1999
    ..The sugar used as substrate by PdxJ is 1-deoxy-D-xylulose-5-phosphate rather than the previously assumed 1-deoxy-D-xylulose. The first vitamin B6 vitamer synthesised is PNP, and not pyridoxine...
  3. ncbi Cloning, purification, and crystallization of Escherichia coli cystathionine beta-lyase
    B Laber
    Hoechst Schering AgrEvo GmbH, Berlin, Germany
    FEBS Lett 379:94-6. 1996
    ..The crystals are suitable for X-ray analysis and a complete native date set to 1.83 A resolution has been collected using synchrotron radiation...
  4. ncbi Structural basis for the function of pyridoxine 5'-phosphate synthase
    M G Franco
    Max Planck Institut fur Biochemie, Abteilung Strukturforschung, D 82152 Planegg Martinsried, Germany
    Structure 9:245-53. 2001
    ..PNP synthase (commonly referred to as PdxJ) is a homooctameric enzyme that catalyzes the final step in this pathway, a complex intramolecular condensation reaction between 1-deoxy-D-xylulose-5'-phosphate and 1-amino-acetone-3-phosphate...
  5. ncbi Crystallization and preliminary X-ray crystallographic analysis of PdxJ, the pyridoxine 5'-phosphate synthesizing enzyme
    M Garrido Franco
    Max Planck Institute of Biochemistry, Department of Structural Investigation, Am Klopferspitz 18a, D 82152 Martinsried, Germany
    Acta Crystallogr D Biol Crystallogr 56:1045-8. 2000
    ..The 12 mercury sites located are related by 222 symmetry and, in combination with self-rotation search analyses and gel-filtration experiments, indicate the quaternary assembly of PdxJ into octamers with 422 symmetry...
  6. ncbi Slow-binding inhibition of Escherichia coli cystathionine beta-lyase by L-aminoethoxyvinylglycine: a kinetic and X-ray study
    T Clausen
    Max Planck Institut fur Biochemie, Abteilung Strukturforschung, Am Klopferspitz 18a, D 82152 Martinsried, Germany
    Biochemistry 36:12633-43. 1997
    ..e. protonation of PLP-C4', resulting in the "dead-end" ketimine PLP derivative. The CBLAVG structure furthermore suggests a binding mode for rhizobitoxine and explains the failure of MVG to inhibit CBL...
  7. ncbi Mode of action of cystathionine beta-lyase
    T Clausen
    Max Planck Institut fur Biochemie, Abteilung Strukturforschung, Martinsried, Germany
    Biol Chem 378:321-6. 1997
    ..In combination with further spectroscopic data, crystallographic evidence permits the formulation of a likely reaction mechanism...
  8. ncbi Reaction mechanism of Escherichia coli dihydrodipicolinate synthase investigated by X-ray crystallography and NMR spectroscopy
    S Blickling
    Max Planck Institut fur Biochemie, Abteilung Strukturforschung, Martinsried, FRG
    Biochemistry 36:24-33. 1997
    ..By this method, (4S)-4-hydroxy-2,3,4,5-tetrahydro-(2S)-dipicolinic acid is identified as the only product. A reaction mechanism for DHDPS is proposed, and important features for inhibition are identified...
  9. ncbi Crystal structure of the pyridoxal-5'-phosphate dependent cystathionine beta-lyase from Escherichia coli at 1.83 A
    T Clausen
    Max Planck Institut fur Biochemie, Abteilung Strukturforschung, Martinsried Germany
    J Mol Biol 262:202-24. 1996
    ..3 A resolution to an R-factor of 16.2%. It suggests that Lys210, the PLP-binding residue, mediates the proton transfer between C alpha and S gamma...
  10. pmc Escherichia coli dihydrodipicolinate synthase. Identification of the active site and crystallization
    B Laber
    Max Planck Institute für Biochemie, Martinsried, Federal Republic of Germany
    Biochem J 288:691-5. 1992
    ..26 nm and c = 11.19 nm. The density of the crystals indicates the presence of a dimer of DHDPS subunits per asymmetric unit...