Research Topics
Genomes and GenesSpecies | Woo Suk HwangSummaryAffiliation: Seoul National University Country: Korea Publications
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Publications
Patient-specific embryonic stem cells derived from human SCNT blastocystsWoo Suk Hwang
College of Veterinary Medicine, Seoul National University, Seoul 151 742, Korea
Science 308:1777-83. 2005..Before clinical use of these cells can occur, preclinical evidence is required to prove that transplantation of differentiated NT-hESCs can be safe, effective, and tolerated...- Evidence of a pluripotent human embryonic stem cell line derived from a cloned blastocystWoo Suk Hwang
College of Veterinary Medicine, Seoul National University, Seoul 151 742, Korea
Science 303:1669-74. 2004..Although we cannot completely exclude the possibility that the cells had a parthenogenetic origin, imprinting analyses support a SCNT origin of the derived human ES cells... - Human embryonic stem cells and therapeutic cloningWoo Suk Hwang
Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul, Korea
J Vet Sci 6:87-96. 2005..This concept is known as "therapeutic cloning". In this review, we describe the derivations of ES cells and cloned ES cells by somatic cell nuclear transfer, and their potential applications in transplantation medicine... - Cloned human embryonic stem cells for tissue repair and transplantationWoo Suk Hwang
Department of Theriogenology and Biotechnology, Seoul National University, Seoul, Korea
Stem Cell Rev 1:99-109. 2005..Our approach opens the door for the use of autologous cells derived from nuclear transfer ES (ntES)-derived cells in transplantation medicine... - Analysis of nuclear reprogramming in cloned miniature pig embryos by expression of Oct-4 and Oct-4 related genesEugine Lee
Department of Theriogenology and Biotechnololgy, College of Veterinary Medicine, Seoul National University, Seoul 151-742, South Korea
Biochem Biophys Res Commun 348:1419-28. 2006.... - Effect of epidermal growth factor in preimplantation development of porcine cloned embryosGab Sang Lee
Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul, South Korea
Mol Reprod Dev 71:45-51. 2005..In conclusion, we demonstrated that EGF and EGFr mRNA are expressed in porcine IVF and SCNT preimplantation embryos, and that EGF increased the quality of blastocysts by increasing total cell numbers in porcine SCNT embryos... - Antiapoptotic and embryotrophic effects of alpha-tocopherol and L-ascorbic acid on porcine embryos derived from in vitro fertilization and somatic cell nuclear transferYeon Woo Jeong
Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, 56-1 Sillim-dong, Gwanak-gu, Seoul 151-742, Korea
Theriogenology 66:2104-12. 2006..23 and 0.17 for control and combined group. In conclusion, we recommend supplementing porcine embryo culture medium with 100microM alpha-tocopherol or 100microM l-ascorbic (as a second choice)... - Production of transgenic cloned piglets from genetically transformed fetal fibroblasts selected by green fluorescent proteinGab Sang Lee
Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Sillim-dong, Kwanak-Ku, Seoul 151-742, South Korea
Theriogenology 63:973-91. 2005..In conclusion, a system for porcine SCNT was developed and led to the successful production of hEPO transgenic cloned piglets... - Anti-apoptotic effect of insulin-like growth factor (IGF)-I and its receptor in porcine preimplantation embryos derived from in vitro fertilization and somatic cell nuclear transferSue Kim
Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul, Korea
Mol Reprod Dev 73:1523-30. 2006.... - Improved in vitro bovine embryo development and increased efficiency in producing viable calves using defined mediaKwang Taek Lim
Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul 151 742, Republic of Korea
Theriogenology 67:293-302. 2007..In conclusion, chemically defined culture media without BSA or FBS improved developmental competence of in vitro cultured bovine embryos and delivery of viable calves after ET... - An approach for producing transgenic cloned cows by nuclear transfer of cells transfected with human alpha 1-antitrypsin geneGoo Jang
Department of Therigenology and Biotechnology, College of Veterinary Medicine, Seoul National University, San56-1, Kwanak-Gu, Seoul 151-742, South Korea
Theriogenology 65:1800-12. 2006..Although we failed to obtain a viable transgenic cloned calf, integration of alpha1-AT gene into the fetus presents the possibility of producing transgenic cloned cows by somatic cell nuclear transfer... - The role of gonadotropin-releasing hormone (GnRH) and its receptor in development of porcine preimplantation embryos derived from in vitro fertilizationDong Hyun Nam
Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul 151-742, South Korea
Theriogenology 63:190-201. 2005..In conclusion, the present study demonstrated that supplementing a culture medium with GnRH agonist can improve blastocyst formation and the quality of porcine IVF embryos, and that this action was mediated through GnRH receptors... - Effect of centrifugation and electrical activation on male pronucleus formation and embryonic development of porcine oocytes reconstructed with intracytoplasmic sperm injectionHwan Yul Yong
Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Korea
Reprod Fertil Dev 17:557-63. 2005..05). In conclusion, the present study clearly demonstrated that oocyte centrifugation before sperm injection is not beneficial to normal fertilisation and that electrical activation is not necessary in the modified porcine ICSI... - Effects of estradiol-17beta and progesterone supplementation on in vitro nuclear maturation of canine oocytesMin Kyu Kim
Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul 151-742, Republic of Korea
Theriogenology 63:1342-53. 2005.... - Endangered wolves cloned from adult somatic cellsMin Kyu Kim
Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul, Korea
Cloning Stem Cells 9:130-7. 2007..In conclusion, we demonstrated live birth of two cloned gray wolves by nuclear transfer of wolf somatic cells into enucleated canine oocyte, indicating that SCNT is a practical approach for conserving endangered canids... - Embryotropic effect of insulin-like growth factor (IGF)-I and its receptor on development of porcine preimplantation embryos produced by in vitro fertilization and somatic cell nuclear transferSue Kim
Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul, Korea
Mol Reprod Dev 72:88-97. 2005..In conclusion, the present study demonstrated that IGF-IR mRNA is expressed in porcine IVF and SCNT embryos, and that IGF-I improved the developmental competence of IVF and SCNT embryos through its specific receptors... - Dogs cloned from adult somatic cellsByeong Chun Lee
Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Gwanak-gu, Seoul 151-742, South Korea
Nature 436:641. 2005.... - Effects of canine serum collected from dogs at different estrous cycle stages on in vitro nuclear maturation of canine oocytesHyun Ju Oh
Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Korea
Zygote 13:227-32. 2005..3% MII) or 20% canine estrous serum (5.1% MII) or the control (2.7% MII). In conclusion, our results demonstrate that supplementing culture medium with 10% canine estrous serum improves IVM of canine follicular stage oocytes... - Embryotrophic effects of ethylenediaminetetraacetic acid and hemoglobin on in vitro porcine embryos developmentJi Hye Kim
Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul 151-742, South Korea
Theriogenology 66:449-55. 2006..In conclusion, the present study clearly demonstrated that the combining treatment of EDTA and Hb improved IVF porcine embryo development... - Sperm movement in the ooplasm, dithiothreitol pretreatment and sperm freezing are not required for the development of porcine embryos derived from injection of head membrane-damaged spermHwan Yul Yong
Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul 151-742, South Korea
Theriogenology 63:783-94. 2005..In conclusion, the present study clearly demonstrated that sperm movement in the ooplasm, use of DTT and fresh spermatozoa did not significantly affect on embryo development in porcine modified ICSI... - Temporal effects of alpha-tocopherol and L-ascorbic acid on in vitro fertilized porcine embryo developmentMohammad Shamim Hossein
Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, 56 1 Sillim Dong, Gwanak Gu, Seoul 151 742, Republic of Korea
Anim Reprod Sci 100:107-17. 2007..Our results demonstrated that the embryotrophic effects of alpha-tocopherol and/or L-ascorbic acid, in terms of frequency of blastocyst formation and number of cells in blastocyst, depends on the concentration and supplementation timing... - Effects of protein source and energy substrates on the in vitro development of bovine embryos in a two-step culture systemKwang Taek Lim
Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul 151 742, Korea
J Vet Sci 4:73-8. 2003..Therefore, the avoidance of this negative effect should allow more optimal conditions to be developed for in vitro production... - Successful surgical correction of anal atresia in a transgenic cloned pigletGab Sang Lee
Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Korea
J Vet Sci 6:243-5. 2005..This cloned piglet seemed to be normal with healthy status after surgery. This report can be useful for the treatment of anal atresia of cloned piglets... - Developmental competence and gene expression in preimplantation bovine embryos derived from somatic cell nuclear transfer using different donor cellsGoo Jang
Department of Theriogenology and Biotechnology, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University, Korea
Zygote 13:187-95. 2005..This may affect the developmental competence of SCNT embryos reconstructed with different cell types after implantation or during fetal growth in vivo... - Characterization of porcine growth differentiation factor-9 and its expression in oocyte maturationGab Sang Lee
Laboratory of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul, Republic of Korea
Mol Reprod Dev 75:707-14. 2008..In conclusion, porcine gdf-9 gene is expressed in the porcine reproductive organ, especially in oocytes and embryos, and its expression is decreased during IVM... - Characterization of pig vasa homolog gene and specific expression in germ cell lineageGab Sang Lee
Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul, Korea
Mol Reprod Dev 72:320-8. 2005..Our results suggest that Pvh gene can be a useful marker for germ cell development in pigs... - Effects of thiol compounds on in vitro maturation of canine oocytes collected from different reproductive stagesMohammad Shamim Hossein
Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul, Republic of Korea
Mol Reprod Dev 74:1213-20. 2007..5 mM cysteine and 100 microM cysteamine were supplemented together. In conclusion, addition of 0.5 mM cysteine and 100 microM cysteamine to the maturation medium improved IVM of canine oocytes... - Role of messenger RNA expression of platelet activating factor and its receptor in porcine in vitro-fertilized and cloned embryo developmentSo Hyun Lee
Department of Theriogenology and Biotechnololgy, College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea
Biol Reprod 71:919-25. 2004..In conclusion, the present study demonstrated that PAFr mRNA was expressed in porcine IVF and SCNT embryos, and that PAF supplement improved the developmental competence of SCNT embryos through its specific receptors... - The beneficial effects of insulin and metformin on in vitro developmental potential of porcine oocytes and embryosMyeong Seop Lee
Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea
Biol Reprod 73:1264-8. 2005..The effects of insulin and metformin were associated with oocyte GSH content and tyrosine kinase activity... - Improved embryo development with decreased apoptosis in blastomeres after the treatment of cloned bovine embryos with beta-mercaptoethanol and hemoglobinEul Soon Park
School of Agricultural Biotechnology, Seoul National University, Seoul, Korea
Mol Reprod Dev 67:200-6. 2004..The use of ME-treated donor cells and the culture of cloned embryos in Hb + ME-containing medium yielded the optimal results for promoting the production of blastocysts with improved quality... - Anti-apoptotic effect of melatonin on preimplantation development of porcine parthenogenetic embryosJiho Choi
SooAm Biotech Research Foundation, Bangbae3 dong, Seocho Gu, Seoul, Korea
Mol Reprod Dev 75:1127-35. 2008..Therefore, these data suggested that melatonin may have important implications for improving porcine preimplantation SCNT embryo development... - Effect of beta-mercaptoethanol or epidermal growth factor supplementation on in vitro maturation of canine oocytes collected from dogs with different stages of the estrus cycleMin Kyu Kim
Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea
J Vet Sci 5:253-8. 2004..Taken together, supplementation of beta-ME (50 or 100 microM) or EGF (20 ng/ml) improved IVM of canine oocytes to MII stage... - Effect of glycosaminoglycans on the preimplantation development of embryos derived from in vitro fertilization and somatic cell nuclear transferGoo Jang
Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul, Korea
Reprod Fertil Dev 15:179-85. 2003..The GAGs increased the quality of blastocysts by increasing total cell numbers in the SCNT embryos... - A modified method for ICSI in the pig: injection of head membrane-damaged sperm using a 3-4 micro m diameter injection pipetteHwan Yul Yong
Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea
Hum Reprod 18:2390-6. 2003..We believe that the present study provides sufficient technical advancement to replace conventional ICSI with the modified ICSI, which is more effective and also avoids unnecessary procedures involved in conventional ICSI... - Age-dependent expression of immune-privilege and proliferation-related molecules on porcine Sertoli cellsHak-Mo Lee
Department of Thoracic and Cardiovascular Surgery, Seoul National University, Seoul, Korea
Xenotransplantation 13:69-74. 2006..These data may be useful for determining the optimal time for harvesting Sertoli cells with respect to maximal immunoprotection and proliferative activity... - The past, present, and future of xenotransplantationCurie Ahn
Department of Internal Medicine, Seoul National University College of Medicine, Seoul, Korea
Yonsei Med J 45:1017-24. 2004 - Choice of the adequate detection time for the accurate evaluation of the efficiency of siRNA-induced gene silencingInho Choi
Laboratory of Immunology, Transplantation Research Institute and Clinical Research Institute, Seoul National University Hospital, Seoul 110-744, Republic of Korea
J Biotechnol 120:251-61. 2005.... - Effects of insulin-transferrin-selenium in defined and porcine follicular fluid supplemented IVM media on porcine IVF and SCNT embryo productionYeon Woo Jeong
SooAm Biotech Research Foundation, Bangbae 3 Dong, Seoul 137 851, Republic of Korea
Anim Reprod Sci 106:13-24. 2008..Thus, we recommended to supplement porcine IVM medium with 10 microg/mL insulin, 5.5 microg/mL transferrin and 5 microg/mL selenium... - Beneficial effects of brain-derived neurotropic factor on in vitro maturation of porcine oocytesEugine Lee
SooAm Biotech Research Foundation, Sooambuilding 1027 4, Bangbae3 dong, Seocho Gu, Seoul 137 851, South Korea
Reproduction 134:405-14. 2007..Also, when used together with EGF, BDNF increased the developmental potency of embryos after IVF and SCNT, demonstrating an improved in vitro production protocol for porcine oocytes... - The analysis of chromatin remodeling and the staining for DNA methylation and histone acetylation do not provide definitive indicators of the developmental ability of inter-species cloned embryosEugine Lee
SooAm Biotech Research Foundation, Seocho Gu, Seoul, Republic of Korea
Anim Reprod Sci 105:438-50. 2008..These results suggest that although partial chromatin remodeling occurs, further investigation is needed to be able to use pig oocytes as recipient oocytes in dog cloning... - Woo Suk HwangAimee Cunningham
Seoul National University
Sci Am 293:48. 2005 - MHC expression in a human adult stem cell line and its down-regulation by hCMV US gene transfectionJae Young Kim
Xenotransplantation Research Center, Seoul National University Hospital, Seoul 110-744, South Korea
Int J Biochem Cell Biol 37:69-78. 2005..In addition, viral stealth mechanisms can be exploited for stem cell transplantation... - Molecular cloning, expression and functional characterization of miniature swine CD86Inho Choi
Xenotransplantation Research Center and Clinical Research Institute, Seoul National University Hospital, Seoul 110 744, South Korea
Mol Immunol 43:480-6. 2006.... - Novel Calbindin-D(9k) protein as a useful biomarker for environmental estrogenic compounds in the uterus of immature ratsBeum-Soo An
Laboratory of Veterinary Biochemistry and Molecular Biology, College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University, Cheongju, 361-763, Chungbuk, Republic of Korea
Reprod Toxicol 17:311-9. 2003..Thus, regarding to risk assessment, we propose that CaBP-9k protein assay in the immature rat uterus can be a very sensitive and powerful tool to identify compounds with estrogenic activity when used in combination with classical assays... - Establishment and characterization of endothelial cell lines from the aorta of miniature pig for the study of xenotransplantationDonghee Kim
Xenotransplantation Research Center and Transplantation Research Institute, Seoul National University Hospital, Republic of Korea
Cell Biol Int 29:638-46. 2005.... - A massive snowball of fraud and deceitSusan Kerr Bernal
J Androl 27:313-5. 2006 - Embryogenesis and blastocyst development after somatic cell nuclear transfer in nonhuman primates: overcoming defects caused by meiotic spindle extractionCalvin Simerly
Department of Obstetrics-Gynecology-Reproductive Sciences, Pittsburgh Development Center, Magee-Womens Research Institute, University of Pittsburgh School of Medicine, 204 Craft Avenue, Pittsburgh, PA 15213, USA
Dev Biol 276:237-52. 2004....