Woo Suk Hwang

Summary

Affiliation: Seoul National University
Country: Korea

Publications

  1. ncbi request reprint Patient-specific embryonic stem cells derived from human SCNT blastocysts
    Woo Suk Hwang
    College of Veterinary Medicine, Seoul National University, Seoul 151 742, Korea
    Science 308:1777-83. 2005
  2. ncbi request reprint Evidence of a pluripotent human embryonic stem cell line derived from a cloned blastocyst
    Woo Suk Hwang
    College of Veterinary Medicine, Seoul National University, Seoul 151 742, Korea
    Science 303:1669-74. 2004
  3. ncbi request reprint Human embryonic stem cells and therapeutic cloning
    Woo Suk Hwang
    Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul, Korea
    J Vet Sci 6:87-96. 2005
  4. ncbi request reprint Cloned human embryonic stem cells for tissue repair and transplantation
    Woo Suk Hwang
    Department of Theriogenology and Biotechnology, Seoul National University, Seoul, Korea
    Stem Cell Rev 1:99-109. 2005
  5. ncbi request reprint Effect of protein supplementation in potassium simplex optimization medium on preimplantation development of bovine non-transgenic and transgenic cloned embryos
    M M U Bhuiyan
    Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul 151 742, South Korea
    Theriogenology 62:1403-16. 2004
  6. ncbi request reprint Embryotropic effect of glycosaminoglycans and receptors in development of porcine pre-implantation embryos
    Hye Soo Kim
    Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul 151 742, South Korea
    Theriogenology 63:1167-80. 2005
  7. ncbi request reprint Analysis of nuclear reprogramming in cloned miniature pig embryos by expression of Oct-4 and Oct-4 related genes
    Eugine Lee
    Department of Theriogenology and Biotechnololgy, College of Veterinary Medicine, Seoul National University, Seoul 151 742, South Korea
    Biochem Biophys Res Commun 348:1419-28. 2006
  8. ncbi request reprint Improved in vitro development of porcine embryos with different energy substrates and serum
    Hye Soo Kim
    Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul 151 742, South Korea
    Theriogenology 61:1381-93. 2004
  9. ncbi request reprint Improvement of a porcine somatic cell nuclear transfer technique by optimizing donor cell and recipient oocyte preparations
    Gab Sang Lee
    Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul 151 742, South Korea
    Theriogenology 59:1949-57. 2003
  10. ncbi request reprint Antiapoptotic and embryotrophic effects of alpha-tocopherol and L-ascorbic acid on porcine embryos derived from in vitro fertilization and somatic cell nuclear transfer
    Yeon Woo Jeong
    Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, 56 1 Sillim Dong, Gwanak Gu, Seoul 151 742, Korea
    Theriogenology 66:2104-12. 2006

Collaborators

  • Byeong Chun Lee
  • Goo Jang
  • Hye Jin Kim
  • Curie Ahn
  • Gerald Schatten
  • Dae Yong Kim
  • Jeong Mook Lim
  • Shin Yong Moon
  • Jae Yong Han
  • Dong Sup Lee
  • Sangho Roh
  • Sun Ha Paek
  • Jose Cibelli
  • Herve Chneiweiss
  • Ho Jae Han
  • M M Uddin Bhuiyan
  • Sung Keun Kang
  • Yeon Woo Jeong
  • Sue Kim
  • Gab Sang Lee
  • Ji Hye Kim
  • Sang Hwan Hyun
  • So Hyun Lee
  • Mohammad Shamim Hossein
  • Eugine Lee
  • Min Kyu Kim
  • Seon Mi Park
  • Sun Woo Park
  • Dong Hyun Nam
  • Hwan Yul Yong
  • Inho Choi
  • Hyun Ju Oh
  • Jong Yun Lee
  • Yeon Ik Jeong
  • Chang Kyu Lee
  • Ok Jae Koo
  • M S Hossein
  • Jae Young Kim
  • Kwang Taek Lim
  • Eun Song Lee
  • Jiho Choi
  • Eu Gine Lee
  • Kyung Jun Uh
  • Byoung Chol Oh
  • Myeong Seop Lee
  • Jung Sang Lee
  • Yuda Heru Fibrianto
  • Ji Young Hong
  • Joung Joo Kim
  • Nam Shik Shin
  • Hak Mo Lee
  • M A Hashem
  • Susan Kerr Bernal
  • Chung Gyu Park
  • Jeong Ryul Lee
  • Aimee Cunningham
  • Hyun Yong Jeon
  • Kyu Seung Lee
  • Calvin Simerly
  • Eul Soon Park
  • Beum Soo An
  • Kyu Chan Hwang
  • Kwang Hwan Choi
  • Taeyoung Shin
  • Nam Hyung Kim
  • Jin Kyu Park
  • Chi Hun Park
  • In Sung Hwang
  • Yeun Wook Kim
  • Dilip P Bhandari
  • Jung Joo Kim
  • Won Wou Lee
  • Fibrianto Yuda
  • Jeong Joo Kim
  • Md Abul Hashem
  • Hee Jung Park
  • Okjae Koo
  • Kyung Hee Ko
  • Hyun Joo Oh
  • Bumrae Cho
  • Doo Hyun Chung
  • Soo Whan Kwon
  • M D Abul Hashem
  • Jaejin Cho
  • Sun Mi Park
  • Jung Tae Kim
  • Gene Lee
  • Sung Dae Kim
  • Dongkyoo Park
  • Hye Young Son

Detail Information

Publications58

  1. ncbi request reprint Patient-specific embryonic stem cells derived from human SCNT blastocysts
    Woo Suk Hwang
    College of Veterinary Medicine, Seoul National University, Seoul 151 742, Korea
    Science 308:1777-83. 2005
    ..Before clinical use of these cells can occur, preclinical evidence is required to prove that transplantation of differentiated NT-hESCs can be safe, effective, and tolerated...
  2. ncbi request reprint Evidence of a pluripotent human embryonic stem cell line derived from a cloned blastocyst
    Woo Suk Hwang
    College of Veterinary Medicine, Seoul National University, Seoul 151 742, Korea
    Science 303:1669-74. 2004
    ..Although we cannot completely exclude the possibility that the cells had a parthenogenetic origin, imprinting analyses support a SCNT origin of the derived human ES cells...
  3. ncbi request reprint Human embryonic stem cells and therapeutic cloning
    Woo Suk Hwang
    Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul, Korea
    J Vet Sci 6:87-96. 2005
    ..This concept is known as "therapeutic cloning". In this review, we describe the derivations of ES cells and cloned ES cells by somatic cell nuclear transfer, and their potential applications in transplantation medicine...
  4. ncbi request reprint Cloned human embryonic stem cells for tissue repair and transplantation
    Woo Suk Hwang
    Department of Theriogenology and Biotechnology, Seoul National University, Seoul, Korea
    Stem Cell Rev 1:99-109. 2005
    ..Our approach opens the door for the use of autologous cells derived from nuclear transfer ES (ntES)-derived cells in transplantation medicine...
  5. ncbi request reprint Effect of protein supplementation in potassium simplex optimization medium on preimplantation development of bovine non-transgenic and transgenic cloned embryos
    M M U Bhuiyan
    Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul 151 742, South Korea
    Theriogenology 62:1403-16. 2004
    ..Moreover, different developmental competence in response to protein supplementation of KSOM was observed between bovine non-transgenic and transgenic cloned embryos...
  6. ncbi request reprint Embryotropic effect of glycosaminoglycans and receptors in development of porcine pre-implantation embryos
    Hye Soo Kim
    Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul 151 742, South Korea
    Theriogenology 63:1167-80. 2005
    ..In conclusion, the present study demonstrated the expression of HA and HP receptors and the embryotrophic effect of HA or HP on porcine IVF embryos...
  7. ncbi request reprint Analysis of nuclear reprogramming in cloned miniature pig embryos by expression of Oct-4 and Oct-4 related genes
    Eugine Lee
    Department of Theriogenology and Biotechnololgy, College of Veterinary Medicine, Seoul National University, Seoul 151 742, South Korea
    Biochem Biophys Res Commun 348:1419-28. 2006
    ....
  8. ncbi request reprint Improved in vitro development of porcine embryos with different energy substrates and serum
    Hye Soo Kim
    Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul 151 742, South Korea
    Theriogenology 61:1381-93. 2004
    ..Our results demonstrated that supplementing pyruvate/lactate containing NCSU-23 medium with 0.4% BSA for 4 days and replacing it with 10% FBS for another 3 days improved porcine IVF embryo development...
  9. ncbi request reprint Improvement of a porcine somatic cell nuclear transfer technique by optimizing donor cell and recipient oocyte preparations
    Gab Sang Lee
    Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul 151 742, South Korea
    Theriogenology 59:1949-57. 2003
    ..A system with good reproducibility has been established using fetal fibroblasts as the donor karyoplast after subculturing 1-10 times, and using both in vivo and in vitro-matured oocytes as the recipient cytoplast...
  10. ncbi request reprint Antiapoptotic and embryotrophic effects of alpha-tocopherol and L-ascorbic acid on porcine embryos derived from in vitro fertilization and somatic cell nuclear transfer
    Yeon Woo Jeong
    Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, 56 1 Sillim Dong, Gwanak Gu, Seoul 151 742, Korea
    Theriogenology 66:2104-12. 2006
    ..23 and 0.17 for control and combined group. In conclusion, we recommend supplementing porcine embryo culture medium with 100microM alpha-tocopherol or 100microM l-ascorbic (as a second choice)...
  11. ncbi request reprint Effect of epidermal growth factor in preimplantation development of porcine cloned embryos
    Gab Sang Lee
    Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul, South Korea
    Mol Reprod Dev 71:45-51. 2005
    ..In conclusion, we demonstrated that EGF and EGFr mRNA are expressed in porcine IVF and SCNT preimplantation embryos, and that EGF increased the quality of blastocysts by increasing total cell numbers in porcine SCNT embryos...
  12. ncbi request reprint Improved developmental competence of cloned porcine embryos with different energy supplements and chemical activation
    Gab Sang Lee
    College of Veterinary Medicine, Seoul National University, Seoul, Korea
    Mol Reprod Dev 66:17-23. 2003
    ..Our results indicate that supplementing NCSU-23 medium with lactate/pyruvate and exposure of cytochalasin B after electrical stimulus can improve in vitro developmental competence of porcine SCNT embryos...
  13. ncbi request reprint An approach for producing transgenic cloned cows by nuclear transfer of cells transfected with human alpha 1-antitrypsin gene
    Goo Jang
    Department of Therigenology and Biotechnology, College of Veterinary Medicine, Seoul National University, San56 1, Kwanak gu, Seoul 151 742, South Korea
    Theriogenology 65:1800-12. 2006
    ..Although we failed to obtain a viable transgenic cloned calf, integration of alpha1-AT gene into the fetus presents the possibility of producing transgenic cloned cows by somatic cell nuclear transfer...
  14. ncbi request reprint Anti-apoptotic effect of insulin-like growth factor (IGF)-I and its receptor in porcine preimplantation embryos derived from in vitro fertilization and somatic cell nuclear transfer
    Sue Kim
    Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul, Korea
    Mol Reprod Dev 73:1523-30. 2006
    ....
  15. ncbi request reprint Improved in vitro bovine embryo development and increased efficiency in producing viable calves using defined media
    Kwang Taek Lim
    Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul 151 742, Republic of Korea
    Theriogenology 67:293-302. 2007
    ..In conclusion, chemically defined culture media without BSA or FBS improved developmental competence of in vitro cultured bovine embryos and delivery of viable calves after ET...
  16. ncbi request reprint Production of transgenic cloned piglets from genetically transformed fetal fibroblasts selected by green fluorescent protein
    Gab Sang Lee
    Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Sillim dong, Kwanak Ku, Seoul 151 742, South Korea
    Theriogenology 63:973-91. 2005
    ..In conclusion, a system for porcine SCNT was developed and led to the successful production of hEPO transgenic cloned piglets...
  17. ncbi request reprint Preimplantational embryo development and incidence of blastomere apoptosis in bovine somatic cell nuclear transfer embryos reconstructed with long-term cultured donor cells
    Goo Jang
    Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul 151 742, South Korea
    Theriogenology 62:512-21. 2004
    ..In conclusion, these results indicated that the long-term culture of donor cells caused increased the incidence of apoptosis in bovine SCNT embryos but did not affect the developmental competence and the cell number of blastocysts...
  18. ncbi request reprint Effects of canine serum collected from dogs at different estrous cycle stages on in vitro nuclear maturation of canine oocytes
    Hyun Ju Oh
    Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Korea
    Zygote 13:227-32. 2005
    ..3% MII) or 20% canine estrous serum (5.1% MII) or the control (2.7% MII). In conclusion, our results demonstrate that supplementing culture medium with 10% canine estrous serum improves IVM of canine follicular stage oocytes...
  19. ncbi request reprint Expression of leptin ligand and receptor and effect of exogenous leptin supplement on in vitro development of porcine embryos
    Hye Soo Kim
    Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul 151 742, South Korea
    Theriogenology 65:831-44. 2006
    ..In conclusion, the present study demonstrated the expression of leptin ligand and receptor and the embryotropic effect of leptin in SCNT embryos...
  20. ncbi request reprint Effects of estradiol-17beta and progesterone supplementation on in vitro nuclear maturation of canine oocytes
    Min Kyu Kim
    Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul 151 742, Republic of Korea
    Theriogenology 63:1342-53. 2005
    ....
  21. ncbi request reprint Effect of centrifugation and electrical activation on male pronucleus formation and embryonic development of porcine oocytes reconstructed with intracytoplasmic sperm injection
    Hwan Yul Yong
    Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Korea
    Reprod Fertil Dev 17:557-63. 2005
    ..05). In conclusion, the present study clearly demonstrated that oocyte centrifugation before sperm injection is not beneficial to normal fertilisation and that electrical activation is not necessary in the modified porcine ICSI...
  22. ncbi request reprint The role of gonadotropin-releasing hormone (GnRH) and its receptor in development of porcine preimplantation embryos derived from in vitro fertilization
    Dong Hyun Nam
    Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul 151 742, South Korea
    Theriogenology 63:190-201. 2005
    ..In conclusion, the present study demonstrated that supplementing a culture medium with GnRH agonist can improve blastocyst formation and the quality of porcine IVF embryos, and that this action was mediated through GnRH receptors...
  23. ncbi request reprint Endangered wolves cloned from adult somatic cells
    Min Kyu Kim
    Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul, Korea
    Cloning Stem Cells 9:130-7. 2007
    ..In conclusion, we demonstrated live birth of two cloned gray wolves by nuclear transfer of wolf somatic cells into enucleated canine oocyte, indicating that SCNT is a practical approach for conserving endangered canids...
  24. ncbi request reprint Embryotropic effect of insulin-like growth factor (IGF)-I and its receptor on development of porcine preimplantation embryos produced by in vitro fertilization and somatic cell nuclear transfer
    Sue Kim
    Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul, Korea
    Mol Reprod Dev 72:88-97. 2005
    ..In conclusion, the present study demonstrated that IGF-IR mRNA is expressed in porcine IVF and SCNT embryos, and that IGF-I improved the developmental competence of IVF and SCNT embryos through its specific receptors...
  25. ncbi request reprint Dogs cloned from adult somatic cells
    Byeong Chun Lee
    Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Gwanak Gu, Seoul 151 742, South Korea
    Nature 436:641. 2005
    ....
  26. doi request reprint Anti-apoptotic effect of melatonin on preimplantation development of porcine parthenogenetic embryos
    Jiho Choi
    SooAm Biotech Research Foundation, Bangbae3 dong, Seocho Gu, Seoul, Korea
    Mol Reprod Dev 75:1127-35. 2008
    ..Therefore, these data suggested that melatonin may have important implications for improving porcine preimplantation SCNT embryo development...
  27. ncbi request reprint Sperm movement in the ooplasm, dithiothreitol pretreatment and sperm freezing are not required for the development of porcine embryos derived from injection of head membrane-damaged sperm
    Hwan Yul Yong
    Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul 151 742, South Korea
    Theriogenology 63:783-94. 2005
    ..In conclusion, the present study clearly demonstrated that sperm movement in the ooplasm, use of DTT and fresh spermatozoa did not significantly affect on embryo development in porcine modified ICSI...
  28. ncbi request reprint Effects of protein source and energy substrates on the in vitro development of bovine embryos in a two-step culture system
    Kwang Taek Lim
    Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul 151 742, Korea
    J Vet Sci 4:73-8. 2003
    ..Therefore, the avoidance of this negative effect should allow more optimal conditions to be developed for in vitro production...
  29. ncbi request reprint Developmental competence and gene expression in preimplantation bovine embryos derived from somatic cell nuclear transfer using different donor cells
    Goo Jang
    Department of Theriogenology and Biotechnology, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University, Korea
    Zygote 13:187-95. 2005
    ..This may affect the developmental competence of SCNT embryos reconstructed with different cell types after implantation or during fetal growth in vivo...
  30. ncbi request reprint Temporal effects of alpha-tocopherol and L-ascorbic acid on in vitro fertilized porcine embryo development
    Mohammad Shamim Hossein
    Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, 56 1 Sillim Dong, Gwanak Gu, Seoul 151 742, Republic of Korea
    Anim Reprod Sci 100:107-17. 2007
    ..Our results demonstrated that the embryotrophic effects of alpha-tocopherol and/or L-ascorbic acid, in terms of frequency of blastocyst formation and number of cells in blastocyst, depends on the concentration and supplementation timing...
  31. ncbi request reprint Successful surgical correction of anal atresia in a transgenic cloned piglet
    Gab Sang Lee
    Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Korea
    J Vet Sci 6:243-5. 2005
    ..This cloned piglet seemed to be normal with healthy status after surgery. This report can be useful for the treatment of anal atresia of cloned piglets...
  32. ncbi request reprint Embryotrophic effects of ethylenediaminetetraacetic acid and hemoglobin on in vitro porcine embryos development
    Ji Hye Kim
    Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul 151 742, South Korea
    Theriogenology 66:449-55. 2006
    ..In conclusion, the present study clearly demonstrated that the combining treatment of EDTA and Hb improved IVF porcine embryo development...
  33. ncbi request reprint Characterization of pig vasa homolog gene and specific expression in germ cell lineage
    Gab Sang Lee
    Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul, Korea
    Mol Reprod Dev 72:320-8. 2005
    ..Our results suggest that Pvh gene can be a useful marker for germ cell development in pigs...
  34. ncbi request reprint Characterization of porcine growth differentiation factor-9 and its expression in oocyte maturation
    Gab Sang Lee
    Laboratory of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul, Republic of Korea
    Mol Reprod Dev 75:707-14. 2008
    ..In conclusion, porcine gdf-9 gene is expressed in the porcine reproductive organ, especially in oocytes and embryos, and its expression is decreased during IVM...
  35. ncbi request reprint Production of blastocysts after intergeneric nuclear transfer of goral (Naemorhedus goral) somatic cells into bovine oocytes
    Byoung Chol Oh
    Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, and The Xenotransplantation Research Center, Seoul National University Hospital, Korea
    J Vet Med Sci 68:1167-71. 2006
    ..The total cell number of goral intergeneric embryos averaged 130.3 (range 105-180). In conclusion, this study demonstrated that bovine oocytes can support blastocyst development after intergeneric SCNT with goral fibroblasts...
  36. ncbi request reprint Role of messenger RNA expression of platelet activating factor and its receptor in porcine in vitro-fertilized and cloned embryo development
    So Hyun Lee
    Department of Theriogenology and Biotechnololgy, College of Veterinary Medicine, Seoul National University, Seoul 151 742, Korea
    Biol Reprod 71:919-25. 2004
    ..In conclusion, the present study demonstrated that PAFr mRNA was expressed in porcine IVF and SCNT embryos, and that PAF supplement improved the developmental competence of SCNT embryos through its specific receptors...
  37. ncbi request reprint Effects of thiol compounds on in vitro maturation of canine oocytes collected from different reproductive stages
    Mohammad Shamim Hossein
    Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul, Republic of Korea
    Mol Reprod Dev 74:1213-20. 2007
    ..5 mM cysteine and 100 microM cysteamine were supplemented together. In conclusion, addition of 0.5 mM cysteine and 100 microM cysteamine to the maturation medium improved IVM of canine oocytes...
  38. ncbi request reprint The beneficial effects of insulin and metformin on in vitro developmental potential of porcine oocytes and embryos
    Myeong Seop Lee
    Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul 151 742, Korea
    Biol Reprod 73:1264-8. 2005
    ..The effects of insulin and metformin were associated with oocyte GSH content and tyrosine kinase activity...
  39. ncbi request reprint Improved embryo development with decreased apoptosis in blastomeres after the treatment of cloned bovine embryos with beta-mercaptoethanol and hemoglobin
    Eul Soon Park
    School of Agricultural Biotechnology, Seoul National University, Seoul, Korea
    Mol Reprod Dev 67:200-6. 2004
    ..The use of ME-treated donor cells and the culture of cloned embryos in Hb + ME-containing medium yielded the optimal results for promoting the production of blastocysts with improved quality...
  40. ncbi request reprint Influence of somatic cell donor breed on reproductive performance and comparison of prenatal growth in cloned canines
    Yeon Woo Jeong
    SooAm Biotech Research Foundation, Seoul, Republic of Korea Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul, Republic of Korea
    Theriogenology 81:1207-1213.e1. 2014
    ..Taken together, the efficiency of somatic cell cloning and fetal survival after embryo transfer may be affected significantly by selecting the appropriate genotype. ..
  41. ncbi request reprint Effect of glycosaminoglycans on the preimplantation development of embryos derived from in vitro fertilization and somatic cell nuclear transfer
    Goo Jang
    Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul, Korea
    Reprod Fertil Dev 15:179-85. 2003
    ..The GAGs increased the quality of blastocysts by increasing total cell numbers in the SCNT embryos...
  42. ncbi request reprint A modified method for ICSI in the pig: injection of head membrane-damaged sperm using a 3-4 micro m diameter injection pipette
    Hwan Yul Yong
    Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul 151 742, Korea
    Hum Reprod 18:2390-6. 2003
    ..Conventional ICSI to date was focused only on tail membrane damage to achieve sperm immobilization and disruption of the plasma membrane, even though liberation of soluble sperm factors is achieved by disruption of the sperm head membrane...
  43. ncbi request reprint Effect of beta-mercaptoethanol or epidermal growth factor supplementation on in vitro maturation of canine oocytes collected from dogs with different stages of the estrus cycle
    Min Kyu Kim
    Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul 151 742, Korea
    J Vet Sci 5:253-8. 2004
    ..Taken together, supplementation of beta-ME (50 or 100 microM) or EGF (20 ng/ml) improved IVM of canine oocytes to MII stage...
  44. ncbi request reprint Analysis of imprinted IGF2/H19 gene methylation and expression in normal fertilized and parthenogenetic embryonic stem cells of pigs
    Kyung Jun Uh
    Department of Agricultural Biotechnology, Animal Biotechnology Major, and Research Institute for Agriculture and Life Science, Seoul National University, Seoul, Republic of Korea Sooam Biotech Research Foundation, Seoul, Republic of Korea
    Anim Reprod Sci 147:47-55. 2014
    ..Therefore, with their uni-parental genotype, the pluripotent differentiation potentials of PG ES cells could be a valuable tool for understanding genomic imprinting in embryonic development...
  45. ncbi request reprint Age-dependent expression of immune-privilege and proliferation-related molecules on porcine Sertoli cells
    Hak Mo Lee
    Department of Thoracic and Cardiovascular Surgery, Seoul National University, Seoul, Korea
    Xenotransplantation 13:69-74. 2006
    ..In this study, the time-dependent expressions of immune-privilege- and proliferation-related molecules in Sertoli cells were determined...
  46. ncbi request reprint The past, present, and future of xenotransplantation
    Curie Ahn
    Department of Internal Medicine, Seoul National University College of Medicine, Seoul, Korea
    Yonsei Med J 45:1017-24. 2004
  47. ncbi request reprint Choice of the adequate detection time for the accurate evaluation of the efficiency of siRNA-induced gene silencing
    Inho Choi
    Laboratory of Immunology, Transplantation Research Institute and Clinical Research Institute, Seoul National University Hospital, Seoul 110 744, Republic of Korea
    J Biotechnol 120:251-61. 2005
    ....
  48. ncbi request reprint Woo Suk Hwang
    Aimee Cunningham
    Seoul National University
    Sci Am 293:48. 2005
  49. ncbi request reprint Novel Calbindin-D(9k) protein as a useful biomarker for environmental estrogenic compounds in the uterus of immature rats
    Beum Soo An
    Laboratory of Veterinary Biochemistry and Molecular Biology, College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University, Cheongju, 361 763, Chungbuk, Republic of Korea
    Reprod Toxicol 17:311-9. 2003
    ..Thus, regarding to risk assessment, we propose that CaBP-9k protein assay in the immature rat uterus can be a very sensitive and powerful tool to identify compounds with estrogenic activity when used in combination with classical assays...
  50. ncbi request reprint MHC expression in a human adult stem cell line and its down-regulation by hCMV US gene transfection
    Jae Young Kim
    Xenotransplantation Research Center, Seoul National University Hospital, Seoul 110 744, South Korea
    Int J Biochem Cell Biol 37:69-78. 2005
    ..In addition, viral stealth mechanisms can be exploited for stem cell transplantation...
  51. doi request reprint The analysis of chromatin remodeling and the staining for DNA methylation and histone acetylation do not provide definitive indicators of the developmental ability of inter-species cloned embryos
    Eugine Lee
    SooAm Biotech Research Foundation, Seocho Gu, Seoul, Republic of Korea
    Anim Reprod Sci 105:438-50. 2008
    ..These results suggest that although partial chromatin remodeling occurs, further investigation is needed to be able to use pig oocytes as recipient oocytes in dog cloning...
  52. ncbi request reprint Embryogenesis and blastocyst development after somatic cell nuclear transfer in nonhuman primates: overcoming defects caused by meiotic spindle extraction
    Calvin Simerly
    Department of Obstetrics Gynecology Reproductive Sciences, Pittsburgh Development Center, Magee Womens Research Institute, University of Pittsburgh School of Medicine, 204 Craft Avenue, Pittsburgh, PA 15213, USA
    Dev Biol 276:237-52. 2004
    ....
  53. ncbi request reprint Beneficial effects of brain-derived neurotropic factor on in vitro maturation of porcine oocytes
    Eugine Lee
    SooAm Biotech Research Foundation, Sooambuilding 1027 4, Bangbae3 dong, Seocho Gu, Seoul 137 851, South Korea
    Reproduction 134:405-14. 2007
    ..Also, when used together with EGF, BDNF increased the developmental potency of embryos after IVF and SCNT, demonstrating an improved in vitro production protocol for porcine oocytes...
  54. ncbi request reprint A massive snowball of fraud and deceit
    Susan Kerr Bernal
    J Androl 27:313-5. 2006
  55. ncbi request reprint [Scientific publication and therapeutic cloning now face Hwang mystification: more than a "Cloningate"]
    Herve Chneiweiss
    UMR 752 Inserm Paris 5 Plasticité Gliale, College de France, 11, place Marcellin Berthelot, 75231 Paris Cedex 05, France
    Med Sci (Paris) 22:218-22. 2006
  56. ncbi request reprint Establishment and characterization of endothelial cell lines from the aorta of miniature pig for the study of xenotransplantation
    Donghee Kim
    Xenotransplantation Research Center and Transplantation Research Institute, Seoul National University Hospital, Republic of Korea
    Cell Biol Int 29:638-46. 2005
    ....
  57. ncbi request reprint Molecular cloning, expression and functional characterization of miniature swine CD86
    Inho Choi
    Xenotransplantation Research Center and Clinical Research Institute, Seoul National University Hospital, Seoul 110 744, South Korea
    Mol Immunol 43:480-6. 2006
    ....
  58. ncbi request reprint Effects of insulin-transferrin-selenium in defined and porcine follicular fluid supplemented IVM media on porcine IVF and SCNT embryo production
    Yeon Woo Jeong
    SooAm Biotech Research Foundation, Bangbae 3 Dong, Seoul 137 851, Republic of Korea
    Anim Reprod Sci 106:13-24. 2008
    ..Thus, we recommended to supplement porcine IVM medium with 10 microg/mL insulin, 5.5 microg/mL transferrin and 5 microg/mL selenium...