Research Topics
Genomes and Genes | Sang Yup LeeSummaryAffiliation: Korea Advanced Institute of Science and Technology Country: Korea Publications
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Detail Information
Publications
Production of microbial polyester by fermentation of recombinant microorganismsS Y Lee
Department of Chemical Engineering and BioProcess Engineering Research Center, Korea Advanced Institute of Science and Technology, 373 1 Kusong Dong, Yusong gu, Taejon 305 701, Korea
Adv Biochem Eng Biotechnol 71:183-207. 2001..Novel PHAs have been synthesized by introducing a new PHA biosynthesis pathway or a new PHA synthase gene. In this article, recent advances in the production of PHA by recombinant bacteria are described...- In silico metabolic pathway analysis and design: succinic acid production by metabolically engineered Escherichia coli as an exampleSang Yup Lee
Department of Chemical and Biomolecular Engineering and BioProcess Engineering Research Center, Korea Advanced Institute of Science and Technology, Daejeon, Republic of Korea
Genome Inform 13:214-23. 2002..The results of wet experiments indicate that the pathway predicted by in silico analysis is more efficient than conventional pathway... 
Metabolic engineering of Escherichia coli for production of enantiomerically pure (R)-(--)-hydroxycarboxylic acidsSang Yup Lee
Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology, Yuseong Gu, Daejeon 305 701, Korea
Appl Environ Microbiol 69:3421-6. 2003..This strategy can be used for the production of various enantiomerically pure (R)-hydroxycarboxylic acids from renewable resources...- Microbial cell-surface displaySang Yup Lee
Metabolic and Biomolecular Engineering National Research Laboratory, Daejeon, South Korea
Trends Biotechnol 21:45-52. 2003..Microbial cell-surface display has many potential applications, including live vaccine development, peptide library screening, bioconversion using whole cell biocatalyst and bioadsorption... - Enhanced display of lipase on the Escherichia coli cell surface, based on transcriptome analysisJong Hwan Baek
Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering BK21 Program, BioProcess Engineering Research Center, Dongyang University, Yeongju, Gyeongbuk 750 711, Republic of Korea
Appl Environ Microbiol 76:971-3. 2010..The fused Pseudomonas fluorescens SIK W1 lipase was successfully displayed on the surface of E. coli cells, and the lipase activity could be enhanced by the coexpression of the gadBC genes identified by transcriptome analysis... 
From genome sequence to integrated bioprocess for succinic acid production by Mannheimia succiniciproducensSang Yup Lee
Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering BK21 Program, BioProcess Engineering Research Center, Daejeon, Republic of Korea
Appl Microbiol Biotechnol 79:11-22. 2008..Finally, we finish this mini-review by discussing the issues that need to be addressed to make this process of fermentative succinic acid production employing M. succiniciproducens to reach the industrial-scale process...- Development of sucrose-utilizing Escherichia coli K-12 strain by cloning β-fructofuranosidases and its application for L-threonine productionJeong Wook Lee
Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering BK21 Program, BioProcess Engineering Research Center, Center for Systems and Synthetic Biotechnology, and Institute for BioCentury, KAIST, Daejeon 305 701, Republic of Korea
Appl Microbiol Biotechnol 88:905-13. 2010..284 g L-threonine per g sucrose. This simple approach to make E. coli K-12 to acquire sucrose-utilizing ability and its successful biotechnological application can be employed to develop sustainable bioprocesses using renewable biomass... - Microarray of DNA-protein complexes on poly-3-hydroxybutyrate surface for pathogen detectionTae Jung Park
Department of Chemical and Biomolecular Engineering BK21 Program, Center for Systems and Synthetic Biotechnology, Institute for the BioCentury, KAIST, 335 Gwahangno, Yuseong Gu, Daejeon, 305 701, Republic of Korea
Anal Bioanal Chem 393:1639-47. 2009.... - Metabolic engineering of Clostridium acetobutylicum ATCC 824 for isopropanol-butanol-ethanol fermentationJoungmin Lee
Department of Chemical and Biomolecular Engineering, Institute for the BioCentury, KAIST, Daejeon, Republic of Korea
Appl Environ Microbiol 78:1416-23. 2012..The resulting PJC4BK(pIPA3-Cm2) strain produced 20.4 g/liter of total alcohol. Fermentation could be prolonged by in situ removal of solvents by gas stripping, and 35.6 g/liter of the IBE mixture could be produced in 45 h... - Characterization of a bacterial self-assembly surface layer protein and its application as an electrical nanobiosensorTae Jung Park
BioProcess Engineering Research Center, Institute for the BioCentury, KAIST 335 Gwahangno, Yuseong Gu, Daejeon 305 701, Korea
J Nanosci Nanotechnol 11:402-7. 2011..Bacterial SLP could be used as a biological template for immobilization molecular array, and provides new approaches for nanoelectronic biosensor applications... - Prediction of novel synthetic pathways for the production of desired chemicalsAyoun Cho
Department of Chemical and Biomolecular Engineering BK21 Program, KAIST, Daejeon, South Korea
BMC Syst Biol 4:35. 2010..Also, some of these methods focus on specific pathways, and thus are limited to expansion to the whole metabolism... - Tailor-made type II Pseudomonas PHA synthases and their use for the biosynthesis of polylactic acid and its copolymer in recombinant Escherichia coliTaek Ho Yang
Corporate R and D, LG Chem Research Park, 104 1 Moonji dong, Yuseong Gu, Daejeon 305 380, Republic of Korea
Appl Microbiol Biotechnol 90:603-14. 2011..PLA homopolymer could also be produced at ca. 7 wt.% by employing the several PhaC1 variants containing E130D/S325T/S477G/Q481K quadruple mutations in wild-type E. coli XL1-Blue... - Multi-stage high cell continuous fermentation for high productivity and titerHo Nam Chang
Department of Chemical and Biomolecular Engineering, KAIST Korea Advanced Institute of Science and Technology, Daejeon, Korea
Bioprocess Biosyst Eng 34:419-31. 2011..We suggest MSC-HCDC as a new production platform for various fermentation products including monoclonal antibody... - Metabolic engineering of Escherichia coli for enhanced production of succinic acid, based on genome comparison and in silico gene knockout simulationSang Jun Lee
Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology, 373 1 Guseong dong, Yuseong Gu, Daejeon 305 701, Republic of Korea
Appl Environ Microbiol 71:7880-7. 2005..coli. These results suggest that the comparative genome analysis combined with in silico metabolic analysis can be an efficient way of developing strategies for strain improvement... - WebCell: a web-based environment for kinetic modeling and dynamic simulation of cellular networksDong-Yup Lee
Department of Chemical and Biomolecular Engineering, Metabolic and Biomolecular Engineering National Research Laboratory 373-1 Guseong-dong, Yuseong-gu, Daejeon 305-701, Korea
Bioinformatics 22:1150-1. 2006..A collected model library is also available to provide comprehensive implications for cellular dynamics of the published models... - EcoProDB: the Escherichia coli protein databaseHongseok Yun
Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering, Center for Systems and Synthetic Biotechnology, Institute for the BioCentury, Daejeon 305 701, Korea
Bioinformatics 23:2501-3. 2007..Availability: EcoProDB database system is accessible at http://eecoli.kaist.ac.kr... - Genome-wide identification of the subcellular localization of the Escherichia coli B proteome using experimental and computational methodsMee Jung Han
Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering BK21 Program, BioProcess Engineering Research Center, and Institute for the BioCentury, KAIST, Yuseong Gu, Daejeon, Republic of Korea
Proteomics 11:1213-27. 2011..coli B, and will be useful as a reference for proteome profiling studies under various conditions. The complete proteome data are available online (http://ecolib.kaist.ac.kr)... - Programmable peptide-directed two dimensional arrays of various nanoparticles on graphene sheetsBong Gill Choi
Department of Chem and Biomolecular Eng BK 21, KAIST, 291 Daehak ro, Yuseong Gu, Daejeon, 305 701, Republic of Korea
Nanoscale 3:3208-13. 2011..e., Pt, Au, Pd, and Ru), which would greatly expand the utility and versatility of this approach for the synthesis and array of the advanced nanomaterials... - Advanced cleanup process of the free-flow microfluidic device for protein analysisYun Suk Huh
Department of Chemical and Biomolecular Engineering BK21 Program, Korea Advanced Institute of Science and Technology, Daejeon 305 701, Republic of Korea
Ultramicroscopy 108:1365-70. 2008..The inflow of the purified fusion protein sample can be successfully immobilized on the gold surface and analyzed by confocal fluorescence microscopy and surface plasmon resonance for biotechnological sensors... - Development of label-free optical diagnosis for sensitive detection of influenza virus with genetically engineered fusion proteinTae Jung Park
BioProcess Engineering Research Center, KAIST, 291 Daehak ro, Yuseong Gu, Daejeon 305 701, Republic of Korea
Talanta 89:246-52. 2012..It was used for the detection of influenza virus, an infectious viral disease, as an example case... - Biosynthesis of polyhydroxyalkanoates containing 2-hydroxybutyrate from unrelated carbon source by metabolically engineered Escherichia coliSi Jae Park
Chemical Biotechnology Research Center, Korea Research Institute of Chemical Technology, Yuseong Gu, Daejeon, Republic of Korea
Appl Microbiol Biotechnol 93:273-83. 2012..The metabolic engineering strategy reported here should be useful for the production of PHAs containing 2HB monomer... - Engineering Escherichia coli for increased productivity of serine-rich proteins based on proteome profilingMee-Jung Han
Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering, BioProcess Engineering Research Center, Korea Advanced Institute of Science and Technology, Daejeon 305-701, Republic of Korea
Appl Environ Microbiol 69:5772-81. 2003..The approach taken in this study should be useful in designing a strategy for improving recombinant protein production... - In silico prediction and validation of the importance of the Entner-Doudoroff pathway in poly(3-hydroxybutyrate) production by metabolically engineered Escherichia coliSoon Ho Hong
Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology, 373-1 Guseong-dong, Yuseong-gu, Daejeon 305-701, Republic of Korea
Biotechnol Bioeng 83:854-63. 2003..coli eda gene, which proves the important role of ED pathway on P(3HB) synthesis in recombinant E. coli as predicted by metabolic flux analysis... - Micropatterning proteins on polyhydroxyalkanoate substrates by using the substrate binding domain as a fusion partnerJong Pil Park
Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering, Yuseong-gu, Daejeon, Republic of Korea
Biotechnol Bioeng 92:160-5. 2005..Also, antibody binding assay by surface plasmon resonance suggested that protein-protein interaction studies could be carried out using this system... - Metabolic engineering of Escherichia coli for the production of cadaverine: a five carbon diamineZhi Gang Qian
Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering BK21 Program, BioProcess Engineering Research Center, Center for Systems and Synthetic Biotechnology, and Institute for BioCentury, KAIST, 335 Gwahangno, Yuseong Gu, Daejeon 305 701, Republic of Korea
Biotechnol Bioeng 108:93-103. 2011..61 g L(-1) of cadaverine with a productivity of 0.32 g L(-1) h(-1) by fed-batch cultivation. The strategy reported here should be useful for the bio-based production of cadaverine from renewable resources... - Efficient production of polylactic acid and its copolymers by metabolically engineered Escherichia coliYu Kyung Jung
Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering BK21 Program, Center for Systems and Synthetic Biotechnology, Institute for the BioCentury, KAIST, Daejeon 305 701, Republic of Korea
J Biotechnol 151:94-101. 2011..coli JLXF5 strain was able to produce P(3HB-co-39.6 mol% LA) having the molecular weight of 141,000 Da to 20 g l⁻¹ with a polymer content of 43 wt% in a chemically defined medium by the pH-stat fed-batch culture... - Display of lipase on the cell surface of Escherichia coli using OprF as an anchor and its application to enantioselective resolution in organic solventSeung Hwan Lee
Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering, and BioProcess Engineering Research Center, Korea Advanced Institute of Science and Technology, Yuseong-gu, Daejeon, Republic of Korea
Biotechnol Bioeng 90:223-30. 2005..These results suggest that E. coli cells displaying lipases using OprF as an anchoring motif can be employed for various biotechnological applications both in aqueous and nonaqueous phases... - Characterization of the Arc two-component signal transduction system of the capnophilic rumen bacterium Mannheimia succiniciproducensWon Seok Jung
Omics and Integration Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Korea
FEMS Microbiol Lett 284:109-19. 2008..succiniciproducens ArcB. Our results indicate that different types of signaling molecules and distinct modes of kinase regulation are used by the ArcB proteins of E. coli and M. succiniciproducens... - Transcriptome and proteome analyses of adaptive responses to methyl methanesulfonate in Escherichia coli K-12 and ada mutant strainsJong Hwan Baek
Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering BK21 Program, BioProcess Engineering Research Center, Center for Systems and Synthetic Biotechnology and Institute for the BioCentury, KAIST, Daejeon, Republic of Korea
BMC Microbiol 9:186. 2009..Here we present time-dependent global gene and protein expression profiles following treatment with methyl methanesulfonate (MMS) in E. coli W3110 and its ada mutant strains... - Cell surface display of lipase in Pseudomonas putida KT2442 using OprF as an anchoring motif and its biocatalytic applicationsSeung Hwan Lee
Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology, 373-1 Guseong-dong, Yuseong-gu, Daejeon 305-701, Republic of Korea
Appl Environ Microbiol 71:8581-6. 2005..These results suggest that the OprF anchor can be used for efficient display of proteins in P. putida KT2442 and consequently for various biocatalytic applications... - Flux variability scanning based on enforced objective flux for identifying gene amplification targetsJong Myoung Park
Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering BK21 Program, Center for Systems and Synthetic Biotechnology, Institute for the BioCentury, Korea Advanced Institute of Science and Technology KAIST, Daejeon, 305 701, Republic of Korea
BMC Syst Biol 6:106. 2012..Here, we report a new algorithm which incorporates physiological data into a model to improve the model's prediction capabilities and to capitalize on the relationships between genes and metabolic fluxes... - Systems metabolic engineering of microorganisms for natural and non-natural chemicalsJeong Wook Lee
Department of Chemical and Biomolecular Engineering Brain Korea 21 program, Center for Systems and Synthetic Biotechnology, Institute for the BioCentury, Korea Advanced Institute of Science and Technology, Daejeon, Republic of Korea
Nat Chem Biol 8:536-46. 2012..Finally, we highlight the limitations and challenges to be overcome for the systems metabolic engineering of microorganisms at more advanced levels... - A nanoforest structure for practical surface-enhanced Raman scattering substratesMyeong Lok Seol
Department of Electrical Engineering, KAIST, Daejeon, Republic of Korea
Nanotechnology 23:095301. 2012..Simple fabrication and high throughput with strong in-plane hot-spots imply that the nanoforest structure can be a practical sensing component of a chip-based SERS sensing system... - Continuous butanol production with reduced byproducts formation from glycerol by a hyper producing mutant of Clostridium pasteurianumAlok Malaviya
Department of Chemical and Biomolecular Engineering BK21 Program, Institute for the BioCentury, KAIST, Yuseong Gu, Daejeon, Republic of Korea
Appl Microbiol Biotechnol 93:1485-94. 2012..9 h(-1). This study reports continuous production of butanol with reduced byproducts formation from glycerol using C. pasteurianum, and thus could help design a bioprocess for the improved production of butanol... - Proteome-level responses of Escherichia coli to long-chain fatty acids and use of fatty acid inducible promoter in protein productionMee Jung Han
Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering BK21 Program, Yuseong Gu, Daejeon 305 701, South Korea
J Biomed Biotechnol 2008:735101. 2008..These results suggest that proteomics is playing an important role not only in biological research but also in various biotechnological applications... - Application of systems biology for bioprocess developmentJin Hwan Park
Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering, Center for Systems and Synthetic Biotechnology, Institute for the BioCentury, KAIST, Daejeon 305 701, Korea
Trends Biotechnol 26:404-12. 2008..Here, we review the applications of systems biology for the development of strains and bioprocesses by means of several successful examples and, furthermore, discuss future prospects... - Biosynthesis of enantiopure (S)-3-hydroxybutyric acid in metabolically engineered Escherichia coliSang Hyun Lee
Corporate R and D, LG Chem, Ltd Research Park, Yuseong Gu, Daejeon, Republic of Korea
Appl Microbiol Biotechnol 79:633-41. 2008..7 g l(-1), which also resulted in the decrease of the S3HB productivity to 0.05 g l(-1)h(-1)... - In silico analysis of the effects of H2 and CO2 on the metabolism of a capnophilic bacterium Mannheimia succiniciproducensTae Yong Kim
Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering BK21 Program, Center for Systems and Synthetic Biotechnology, Institute for the BioCentury, 335 Gwahangno, Yuseong Gu, Daejeon 305 701, Republic of Korea
J Biotechnol 144:184-9. 2009.... - Proteome-based physiological analysis of the metabolically engineered succinic acid producer Mannheimia succiniciproducens LPK7Jeong Wook Lee
Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering BK21 Program, BioProcess Engineering Research Center, KAIST, 335 Gwahangno, Yuseong Gu, Daejeon 305 701, Republic of Korea
Bioprocess Biosyst Eng 33:97-107. 2010.... - Development of a markerless gene knock-out system for Mannheimia succiniciproducens using a temperature-sensitive plasmidJi Mahn Kim
Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering BK21 Program, BioProcess Engineering Research Center, Institute for the BioCentury, Daejeon, Korea
FEMS Microbiol Lett 278:78-85. 2008..This markerless deletion method should be useful for further metabolic engineering of M. succiniciproducens, which is a promising industrial bacterium for succinic acid production from renewable resources... - Comparison of the extracellular proteomes of Escherichia coli B and K-12 strains during high cell density cultivationXiao Xia Xia
Department of Chemical and Biomolecular Engineering, BioProcess Engineering Research Center, Institute for the BioCentury, Korea Advanced Institute of Science and Technology KAIST, Yuseong Gu, Daejeon, Republic of Korea
Proteomics 8:2089-103. 2008..These findings summarized as reference proteome maps will be useful for studying protein release in further detail, and provide new strategies for enhanced excretory production of recombinant proteins... - Succinic acid production from continuous fermentation process using Mannheimia succiniciproducens LPK7In Jae Oh
Department of Chemical and Biomolecular Engineering, Sogang University, Seoul 121 742, Korea
J Microbiol Biotechnol 18:908-12. 2008..To examine the quantitative balance of the metabolism, a flux distribution analysis was also performed using the metabolic network model of glycolysis and the pentose phosphate pathway... - Proteome-based identification of fusion partner for high-level extracellular production of recombinant proteins in Escherichia coliZhi Gang Qian
Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering, BioProcess Engineering Research Center, Center for Systems and Synthetic Biotechnology, KAIST, Daejeon, Republic of Korea
Biotechnol Bioeng 101:587-601. 2008..High cell density cultivation allowed production of these proteins to the levels of 250-700 mg/L in the culture medium, suggesting the good potential of this approach for the excretory production of recombinant proteins... - Continuous production of succinic acid using an external membrane cell recycle systemMoon Il Kim
Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology, Daejeon 305 701, Korea
J Microbiol Biotechnol 19:1369-73. 2009..3 times higher, respectively, compared with those obtained at the dilution rate of 0.1 h-1. These observations give a deep insight into the process design for a continuous succinic acid production by microorganisms... - Enhanced proteome profiling by inhibiting proteolysis with small heat shock proteinsMee Jung Han
Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering, BioProcess Engineering Research Center, Daejeon, Republic of Korea
J Proteome Res 4:2429-34. 2005..Therefore, the use of sHsps during 2-DE significantly improves proteome profiling by generally enabling the detection of many more protein spots that could not be seen previously... - Site-specific immobilization of gold binding polypeptide on gold nanoparticle-coated graphene sheet for biosensor applicationMinho Yang
Department of Chemical and Biomolecular Engineering, BK21 Program, KAIST, 291 Daehak ro, Yuseong Gu, Daejeon 305 701, Republic of Korea
Nanoscale 3:2950-6. 2011..This strategy, which integrates biotic and abiotic moieties through site-specific interactions, has a great potential for use in biosensing and bioconversion process... - Data integration and analysis of biological networksTae Yong Kim
Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering BK21 Program, Center for Systems and Synthetic Biotechnology, Institute for the BioCentury, Korea Advanced Institute of Science and Technology KAIST, Daejeon 305 701, Republic of Korea
Curr Opin Biotechnol 21:78-84. 2010..It is expected that this trend will continue, the outcomes of which will allow development of more sophisticated networks integrating diverse omics data, and enhance our understanding of biological systems... - Detection of the most common corneal dystrophies caused by BIGH3 gene point mutations using a multispot gold-capped nanoparticle array chipSo Young Yoo
BioProcess Engineering Research Center, Center for Systems and Synthetic Biotechnology, Institute for the BioCentury, KAIST, 335 Gwahangno, Yuseong Gu, Daejeon 305 701, Republic of Korea
Anal Chem 82:1349-57. 2010.... - Protein nanopatterns and biosensors using gold binding polypeptide as a fusion partnerTae Jung Park
Metabolic and Biomolecular Engineering National Research Laboratory, BioProcess Engineering Research Center, Korea Advanced Institute of Science and Technology (KAIST, Daejeon 305-701, Korea
Anal Chem 78:7197-205. 2006..Furthermore, these studies can be carried out in a microfluidic system, which allows high-throughput analysis of biomolecular interactions... - Genome-based metabolic engineering of Mannheimia succiniciproducens for succinic acid productionSang Jun Lee
Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology, 373 1 Guseong dong, Yuseong Gu, Daejeon 305 701, Republic of Korea
Appl Environ Microbiol 72:1939-48. 2006..4 g/liter of succinic acid, with a succinic acid yield of 1.16 mol succinic acid per mol glucose and a succinic acid productivity of 1.8 g/liter/h, which should be useful for industrial production of succinic acid... - Single-step multiplex detection of toxic metal ions by Au nanowires-on-chip sensor using reporter eliminationTaejoon Kang
Department of Chemistry, KAIST, Daejeon 305 701, Korea
Lab Chip 12:3077-81. 2012..We successfully detected ions of mercury (Hg(2+)), silver (Ag(+)), and lead (Pb(2+)) coexisting in the same solution by using this sensor... - Biosynthesis of lactate-containing polyesters by metabolically engineered bacteriaSi Jae Park
Chemical Biotechnology Research Center, Green Chemistry Division, Korea Research Institute of Chemical Technology, Daejeon, Republic of Korea
Biotechnol J 7:199-212. 2012..Challenges remaining to efficiently produce PLA and its copolymers and strategies to overcome these challenges through metabolic engineering combined with enzyme engineering are discussed... - Prediction of metabolic fluxes by incorporating genomic context and flux-converging pattern analysesJong Myoung Park
Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering, KAIST, Daejeon 305 701, Republic of Korea
Proc Natl Acad Sci U S A 107:14931-6. 2010..Thus, this strategy will be useful for accurately predicting the intracellular fluxes of large metabolic networks when their experimental determination is difficult... - Plasmonic properties of the multispot copper-capped nanoparticle array chip and its application to optical biosensors for pathogen detection of multiplex DNAsDo Kyun Kim
BioProcess Engineering Research Center, Center for Systems and Synthetic Biotechnology, KAIST, Yuseong Gu, Daejeon, Republic of Korea
Anal Chem 83:6215-22. 2011..The optical biosensor developed in this study represents a unique approach to performing LSPR that utilizes a simple and cost-effective optical setup with disposable chips... - Label-free optical diagnosis of hepatitis B virus with genetically engineered fusion proteinsShun Zheng
Department of Chemical and Biomolecular Engineering BK21 Program, KAIST, Yuseong Gu, Daejeon, Republic of Korea
Talanta 82:803-9. 2010.... - Metabolic engineering of microorganisms: general strategies and drug productionSang Yup Lee
Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering, Center for Systems and Synthetic Biotechnology, Institute for the BioCentury, KAIST, Daejeon 305 701, Korea
Drug Discov Today 14:78-88. 2009..Also, systems metabolic engineering of Escherichia coli for the production of L-valine, an important drug precursor, is showcased as an important strategy of future metabolic engineering effort... - Genome-scale metabolic model of methylotrophic yeast Pichia pastoris and its use for in silico analysis of heterologous protein productionSeung Bum Sohn
Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering BK21 Program, Center for Systems and Synthetic Biotechnology, Institute for the BioCentury, KAIST, 335 Gwahangno, Daejeon, Republic of Korea
Biotechnol J 5:705-15. 2010..The protein-producing model versions of PpaMBEL1254 were then analyzed to examine the impact on oxygen limitation on protein production... - Optimization and scale-up of succinic acid production by Mannheimia succiniciproducens LPK7In Jae Oh
Department of Chemical and Biomolecular Engineering, Sogang University, Seoul 121 742, Korea
J Microbiol Biotechnol 19:167-71. 2009..4 g/l and 0.86 g/g. This result shows similar amount of succinic acid obtained in lab-scale fermentation, and it is possible to scale up to larger fermentors without major problems... - Development of anaerobically inducible nar promoter expression vectors for the expression of recombinant proteins in Escherichia coliNag Jong Kim
Department of Chemical and Biomolecular Engineering BK21 Program, Bioinformatics Research Center, BioProcess Engineering Research Center and Center for Ultramicrochemical Process Systems, KAIST, 335 Gwahangno, Yuseong Gu, Daejeon 305 701, Republic of Korea
J Biotechnol 151:102-7. 2011.... - Metabolic engineering of Escherichia coli for the production of putrescine: a four carbon diamineZhi Gang Qian
Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering BK21 Program, BioProcess Engineering Research Center, Center for Systems and Synthetic Biotechnology, Institute for the BioCentury, KAIST, 335 Gwahangno, Yuseong Gu, Daejeon 305 701, Republic of Korea
Biotechnol Bioeng 104:651-62. 2009.... - Fed-batch culture of Escherichia coli for L-valine production based on in silico flux response analysisJin Hwan Park
Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering, BK21 Program, KAIST, Daejeon 305 701, Republic of Korea
Biotechnol Bioeng 108:934-46. 2011..coli. This approach of employing systems-level analysis of metabolic fluxes in developing fed-batch cultivation strategy would also be applicable in developing strategies for the efficient production of other bioproducts... - Development of a fully integrated microfluidic system for sensing infectious viral diseaseYun Suk Huh
Department of Chemical and Biomolecular Engineering BK21 Program, KAIST, Yuseong Gu, Daejeon, Korea
Electrophoresis 29:2960-9. 2008..It was used for the detection of severe acute respiratory syndrome (SARS), an infectious viral disease, as an example case... - Genome-scale analysis of Mannheimia succiniciproducens metabolismTae Yong Kim
Department of Chemical and Biomolecular Engineering BK21 Program, Metabolic and Biomolecular Engineering National Research Laboratory, Korea Advanced Institute of Science and Technology, Daejeon, Republic of Korea
Biotechnol Bioeng 97:657-71. 2007..succiniciproducens to various environmental and genetic perturbations and consequently for designing rational strategies for strain improvement... - Biosynthesis of R-3-hydroxyalkanoic acids by metabolically engineered Escherichia coliSi Jae Park
Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering, BioProcess Engineering Research Center, Republic of Korea
Appl Biochem Biotechnol 113:373-9. 2004..When the R. eutropha intracellular depolymerase gene was expressed by using a stable plasmid containing the hok/sok locus of plasmid R1, R3HB could be efficiently produced... - Knowledge representation model for systems-level analysis of signal transduction networksDong Yup Lee
Department of Chemical and Biomolecular Engineering and Bioinformatics Research Center, Korea Advanced Institute of Science and Technology, 373 1 Guseong dong, Yuseong Gu, Daejeon 305 701, Republic of Korea
Genome Inform 15:234-43. 2004..This strategy should be useful in drug target discovery and its validation... - The proteome of Mannheimia succiniciproducens, a capnophilic rumen bacteriumJeong Wook Lee
Department of Chemical and Biomolecular Engineering, Metabolic and Biomolecular Engineering National Research Laboratory, BioProcess Engineering Research Center, Korea Advanced Institute of Science and Technology, Daejeon, Republic of Korea
Proteomics 6:3550-66. 2006..Comparative proteome profiling revealed valuable information to understand physiological changes during growth, and subsequently suggested target genes to be manipulated for the strain improvement... - Development of chemically defined medium for Mannheimia succiniciproducens based on its genome sequenceHyohak Song
Department of Chemical and Biomolecular Engineering BK21 Program, KAIST, 335 Gwahangno, Yuseong Gu, Daejeon 305 701, Republic of Korea
Appl Microbiol Biotechnol 79:263-72. 2008..The strategy reported in this paper should be generally applicable to the development of CDMs for other organisms, whose genome sequences are available... - Spore display using Bacillus thuringiensis exosporium protein InhATae Jung Park
Department of Chemical and Biomolecular Engineering BK21 Program, BioProcess Engineering Research Center, Center for Systems and Synthetic Biotechnology, and Institute for the BioCentury, KAIST, Daejeon 305 701, Korea
J Microbiol Biotechnol 19:495-501. 2009..InhA-mediated anchoring of foreign proteins in the exosporium of Bacillus spores can provide a new method of microbial display, thereby broadening the potential for novel applications of microbial display... - Systems metabolic engineering for chemicals and materialsJeong Wook Lee
Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering BK21 Program, Center for Systems and Synthetic Biotechnology, Institute for the BioCentury, KAIST, 291 Daehak ro, Yuseong Gu, Daejeon 305 701, Korea
Trends Biotechnol 29:370-8. 2011..Here, we review the recent trends in systems metabolic engineering for the production of chemicals and materials by presenting general strategies and showcasing representative examples... - Alignment of SWNTs by protein-ligand interaction of functionalized magnetic particles under low magnetic fieldsTae Jung Park
BioProcess Engineering Research Center, Institute for the BioCentury, KAIST 335 Gwahangno, Yuseong Gu, Daejeon 305 701, Korea
J Nanosci Nanotechnol 11:4540-5. 2011..We suggest that this approach, which consists of a combination of biological interaction among streptavidin-biotin and magnetite particles, should be useful for lateral orientation of individual SWNTs with controllable direction... - In silico genome-scale metabolic analysis of Pseudomonas putida KT2440 for polyhydroxyalkanoate synthesis, degradation of aromatics and anaerobic survivalSeung Bum Sohn
Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering BK21 Program, Center for Systems and Synthetic Biotechnology, Institute for the BioCentury, KAIST, Daejeon, Republic of Korea
Biotechnol J 5:739-50. 2010..Through analysis of PpuMBEL1071, extended survival of P. putida under anaerobic stress was achieved by introducing the ackA gene from Pseudomonas aeruginosa and Escherichia coli... - Combined transcriptome and proteome analysis of Escherichia coli during high cell density cultureSung Ho Yoon
Department of Chemical and Biomolecular Engineering, and BioProcess Engineering Research Center, Korea Advanced Institute of Science and Technology, 373-1 Guseong-dong, Yuseong-gu, Daejeon 305-701, Korea
Biotechnol Bioeng 81:753-67. 2003..These findings should be invaluable in designing metabolic engineering and fermentation strategies for the production of recombinant proteins and metabolites by HCDC of E. coli... - Excretion of human beta-endorphin into culture medium by using outer membrane protein F as a fusion partner in recombinant Escherichia coliKi Jun Jeong
Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering and BioProcess Engineering Research Center, Korea Advanced Institute of Science and Technology, Yuseong-gu, Daejeon 305-701, Korea
Appl Environ Microbiol 68:4979-85. 2002..From 2.7 liters of culture supernatant, 545.4 mg of beta-endorphin was obtained... - Construction and optimization of synthetic pathways in metabolic engineeringDoKyun Na
Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering BK21 Program, Korea Advanced Institute of Science and Technology, Daejeon 305 701, Republic of Korea
Curr Opin Microbiol 13:363-70. 2010.... - Biosynthesis of poly(3-hydroxybutyrate- co-3-hydroxyalkanoates) by metabolically engineered Escherichia coli strainsSi Jae Park
Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering, BioProcess Engineering Research Center, Republic of Korea
Appl Biochem Biotechnol 113:335-46. 2004..coli strains constructed in this study should be useful for the production of PHAs suitable for various commercial applications... - Construction and characterization of shuttle vectors for succinic acid-producing rumen bacteriaYu Sin Jang
Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering BK21 Program and BioProcess Engineering Research Center, Republic of Korea
Appl Environ Microbiol 73:5411-20. 2007..Thus, the shuttle vectors developed in this study should be useful for genetic and metabolic engineering of succinic acid-producing rumen bacteria... - The genome sequence of the capnophilic rumen bacterium Mannheimia succiniciproducensSoon Ho Hong
Department of Chemical and Biomolecular Engineering, BioProcess Engineering Research Center, Rese Korea Advanced Institute of Science and Technology, Yuseong Gu, Daejeon, Republic of Korea
Nat Biotechnol 22:1275-81. 2004..This characteristic metabolism allows highly efficient production of succinic acid, an important four-carbon industrial chemical... - Systems biotechnologySang Yup Lee
Department of Chemical and Biomolecular Engineering BK21 Program, BioProcess Engineering Research Center, Bioinformatics Research Center, Center for Systems and Synthetic Biotechnology, Institute for the BioCentury, KAIST, Daejeon 305 701, Korea
Genome Inform 23:214-6. 2009..It is expected that systems biotechnology will be at the heart of successful industrial biotechnology towards low carbon green growth of the world... - DNA microarray for the identification of pathogens causing bloodstream infectionsSeung Min Yoo
Department of Chemical and Biomolecular Engineering BK21 program and Bioinformatics Research Center, KAIST, 335 Gwahangno, Yuseong Gu, Daejeon 305 701, Republic of Korea
Expert Rev Mol Diagn 10:263-8. 2010..In this article, we review recently published microarray-based diagnostic studies for accurate and rapid identification of pathogens infected in bloodstream and discuss the advantages and limitations over conventional diagnostic methods... - Systems biotechnology for strain improvementSang Yup Lee
Metabolic and Biomolecular Engineering National Research Laboratory and Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology, 373 1 Guseong dong, Yuseong Gu, Daejeon 305 701, Korea
Trends Biotechnol 23:349-58. 2005..This article highlights the recent developments in these systems approaches, which we call systems biotechnology, and discusses future prospects... - Escherichia coli W as a new platform strain for the enhanced production of L-valine by systems metabolic engineeringJin Hwan Park
Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering BK21 Program, KAIST, Daejeon, Republic of Korea
Biotechnol Bioeng 108:1140-7. 2011..coli K-12, which have so far been the most efficient L-valine producer. Furthermore, it is expected that various bioproducts including other amino acids might be more efficiently produced by this revisited platform strain of E. coli... - Integration of systems biology with bioprocess engineering: L: -threonine production by systems metabolic engineering of Escherichia coliSang Yup Lee
Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering BK21 Program, Center for Systems and Synthetic Biotechnology, Institute for the BioCentury, KAIST, 335 Gwahangno, Yuseong Gu, Daejeon, 305 701, Republic of Korea
Adv Biochem Eng Biotechnol 120:1-19. 2010..Here we review strategies and applications of systems biology successfully implemented on bioprocess engineering, with particular focus on developing L: -threonine production strains of Escherichia coli... - Complete genome sequence of the metabolically versatile plant growth-promoting endophyte Variovorax paradoxus S110Jong In Han
Department of Civil and Environmental Engineering, KAIST, Republic of Korea
J Bacteriol 193:1183-90. 2011..Because it has many associations with other biota, it is also suited to serve as an additional model system for studies of microbe-plant and microbe-microbe interactions... - Novel gene members in the Pho regulon of Escherichia coliJong Hwan Baek
Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering BK21 Program, Daejeon, Korea
FEMS Microbiol Lett 264:104-9. 2006..These results indicate the additional roles of PhoB as a global regulator... - New time-scale criteria for model simplification of bio-reaction systemsJunwon Choi
Department of Chemical and Biomolecular Engineering BK21 Program, KAIST, 335 Gwahangro, Yuseong Gu, Daejeon, 305 701, Republic of Korea
BMC Bioinformatics 9:338. 2008..A more efficient estimation approach is necessary for complex systems... - Fermentative butanol production by ClostridiaSang Yup Lee
Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering BK21 Program, Korea Advanced Institute of Science and Technology, Yuseong Gu, Daejeon 305 701, Republic of Korea
Biotechnol Bioeng 101:209-28. 2008..The strategies for strain improvement by metabolic engineering and further requirements to make fermentative butanol production a successful industrial process are also discussed... - Microbial small heat shock proteins and their use in biotechnologyMee Jung Han
Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology KAIST, Yuseong Gu, Daejeon 305 701, Republic of Korea
Biotechnol Adv 26:591-609. 2008..In this review, we discuss the properties and diversity of microbial sHsps, as well as their potential uses in the biotechnology industry... - Incorporating metabolic flux ratios into constraint-based flux analysis by using artificial metabolites and converging ratio determinantsHyung Seok Choi
Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering, BioProcess Engineering Research Center, Institute for the BioCentury, Yuseong Gu, Daejeon, Republic of Korea
J Biotechnol 129:696-705. 2007..These new mass balance equations were used as additional constraints during the constraint-based flux analysis with genome-scale E. coli metabolic model, which allowed more accurate determination of intracellular metabolic fluxes... - Microarrays of peptides elevated on the protein layer for efficient protein kinase assaySeok Jae Lee
Metabolic and Biomolecular Engineering National Research Laboratory, BioProcess Engineering Research Center, Korea Advanced Institute of Science and Technology, 373 1 Guseong dong, Yuseong Gu, Daejeon, 305 701, Republic of Korea
Anal Biochem 330:311-6. 2004..Therefore, this new strategy will not only be useful in high-throughput and cost-effective screening of kinase substrate peptides but also be generally applicable in studying various protein-peptide interactions... - Display of bacterial lipase on the Escherichia coli cell surface by using FadL as an anchoring motif and use of the enzyme in enantioselective biocatalysisSeung Hwan Lee
Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology, 373 1 Guseong dong, Yuseong Gu, Daejeon 305 701, Republic of Korea
Appl Environ Microbiol 70:5074-80. 2004..These results suggest that FadL may be a useful anchoring motif for displaying enzymes on the cell surface of E. coli for whole-cell biocatalysis... - The genome-scale metabolic network analysis of Zymomonas mobilis ZM4 explains physiological features and suggests ethanol and succinic acid production strategiesKyung Yun Lee
Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering BK21 Program, KAIST, 335 Gwahangno, Yuseong Gu, Daejeon 305 701, Republic of Korea
Microb Cell Fact 9:94. 2010..mobilis is required to systematically analyze in silico the metabolic characteristics of this bacterium under a wide range of genotypic and environmental conditions... - Microwave-assisted synthesis of highly water-soluble graphene towards electrical DNA sensorBong Gill Choi
Department of Chemical and Biomolecular Engineering BK21, KAIST, 335 Gwahangno, Yuseong Gu, Daejeon 305 701, Republic of Korea
Nanoscale 2:2692-7. 2010.... - Constitutive production of human leptin by fed-batch culture of recombinant rpoS- Escherichia coliKi Jun Jeong
Department of Chemical and Biomolecular Engineering and BioProcess Engineering Research Center, Korea Advanced Institute of Science and Technology, 373-1 Guseong-dong, Yuseong-gu, Daejeon 305-701, Republic of Korea
Protein Expr Purif 36:150-6. 2004..These results suggest that the HCE promoter can be used for the efficient production of leptin, and most likely other recombinant proteins, in a constitutive manner... - MetaFluxNet: the management of metabolic reaction information and quantitative metabolic flux analysisDong-Yup Lee
Metabolic and Biomolecular Engineering National Research Laboratory, Korea Advanced Institute of Science and Technology, 373-1 Guseong-dong, Yuseong-gu, Daejeon 305-701, Korea
Bioinformatics 19:2144-6. 2003..AVAILABILITY: http://mbel.kaist.ac.kr/ Supplementary information: A manual for MetaFluxNet is available as PDF file... - Enhanced production of insulin-like growth factor I fusion protein in Escherichia coli by coexpression of the down-regulated genes identified by transcriptome profilingJong Hyun Choi
Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering and BioProcess Engineering Research Center, Daejeon 305-701, Korea
Appl Environ Microbiol 69:4737-42. 2003..36 +/- 0.027 to 0.82 +/- 0.048 g/liter/h. These results demonstrate that transcriptome profiling can provide invaluable information in designing engineered strains showing enhanced performance... - MFAML: a standard data structure for representing and exchanging metabolic flux modelsHongseok Yun
Bioinformatics Research Center, Korea Advanced Institute of Science and Technology, 373-1 Guseong-dong, Yuseong-gu, Daejeon 305-701, Korea
Bioinformatics 21:3329-30. 2005..AVAILABILITY: MFAML (version 1) is fully described and available at http://mbel.kaist.ac.kr/mfaml/... - Development of a whole-cell biosensor by cell surface display of a gold-binding polypeptide on the gold surfaceTae Jung Park
BioProcess Engineering Research Center, Center for Systems and Synthetic Biotechnology, Institute for the BioCentury, KAIST, Daejeon, Korea
FEMS Microbiol Lett 293:141-7. 2009.... - Biotechnological applications of microbial proteomesMee Jung Han
Department of Chemical and Biomolecular Engineering, Dongyang University, 1 Gyochon dong, Punggi eup, Yeongju, Gyeongbuk 750 711, Republic of Korea
J Biotechnol 145:341-9. 2010..Successful applications of proteomic information have proven to have enormous value for both scientific and practical applications... - Systems metabolic engineering of Escherichia coli for L-threonine productionKwang Ho Lee
Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering BK21 Program, BioProcess Engineering Research Center, Korea Advanced Institute of Science and Technology KAIST, Daejeon, Korea
Mol Syst Biol 3:149. 2007..4 g/l Thr by fed-batch culture. The systems metabolic engineering strategy reported here may be broadly employed for developing genetically defined organisms for the efficient production of various bioproducts... - A physiology study of Escherichia coli overexpressing phosphoenolpyruvate carboxykinaseYeong Deok Kwon
Department of Life Science, Catholic University of Korea, Bucheon, Gyunggi 420 743, Korea
Biosci Biotechnol Biochem 72:1138-41. 2008..This unexpected result of higher ATP coupled with slower growth is discussed based on transcriptome analysis... - Recovery of succinic acid produced by fermentation of a metabolically engineered Mannheimia succiniciproducens strainHyohak Song
Metabolic and Biomolecular Engineering National Research Laboratory, Institute for the BioCentury, Korea Advanced Institute of Science and Technology KAIST, 335 Gwahangno, Yuseong Gu, Daejeon 305 701, Republic of Korea
J Biotechnol 132:445-52. 2007....