Sang Yup Lee

Summary

Affiliation: Korea Advanced Institute of Science and Technology
Country: Korea

Publications

  1. doi request reprint Proteome-based identification of fusion partner for high-level extracellular production of recombinant proteins in Escherichia coli
    Zhi Gang Qian
    Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering, BioProcess Engineering Research Center, Center for Systems and Synthetic Biotechnology, KAIST, Daejeon, Republic of Korea
    Biotechnol Bioeng 101:587-601. 2008
  2. ncbi request reprint Systems biotechnology
    Sang Yup Lee
    Department of Chemical and Biomolecular Engineering BK21 Program, BioProcess Engineering Research Center, Bioinformatics Research Center, Center for Systems and Synthetic Biotechnology, Institute for the BioCentury, KAIST, Daejeon 305 701, Korea
    Genome Inform 23:214-6. 2009
  3. doi request reprint Editorial: Latest methods and advances in biotechnology
    Sang Yup Lee
    Biotechnol J 9:2-4. 2014
  4. pmc Comparative multi-omics systems analysis of Escherichia coli strains B and K-12
    Sung Ho Yoon
    Systems and Synthetic Biology Research Center, Korea Research Institute of Bioscience and Biotechnology, Yuseong, Daejeon 305 806, Korea
    Genome Biol 13:R37. 2012
  5. pmc Framework for network modularization and Bayesian network analysis to investigate the perturbed metabolic network
    Hyun Uk Kim
    Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology KAIST, Daejeon 305 701, Republic of Korea
    BMC Syst Biol 5:S14. 2011
  6. doi request reprint Editorial: Flavors of international biotechnology
    Sang Yup Lee
    Biotechnol J 8:754-5. 2013
  7. pmc Genome-scale metabolic model of the fission yeast Schizosaccharomyces pombe and the reconciliation of in silico/in vivo mutant growth
    Seung Bum Sohn
    Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering BK21 Program, Center for Systems and Synthetic Biotechnology, Institute for the BioCentury, KAIST, Daejeon, Republic of Korea
    BMC Syst Biol 6:49. 2012
  8. pmc The genome-scale metabolic network analysis of Zymomonas mobilis ZM4 explains physiological features and suggests ethanol and succinic acid production strategies
    Kyung Yun Lee
    Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering BK21 Program, KAIST, 335 Gwahangno, Yuseong Gu, Daejeon 305 701, Republic of Korea
    Microb Cell Fact 9:94. 2010
  9. pmc Genome-scale reconstruction and in silico analysis of the Ralstonia eutropha H16 for polyhydroxyalkanoate synthesis, lithoautotrophic growth, and 2-methyl citric acid production
    Jong Myoung Park
    Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering BK21 Program, KAIST, 291 Daehak ro, Yuseong Gu, Daejeon 305 701, Republic of Korea
    BMC Syst Biol 5:101. 2011
  10. pmc The genome sequence of E. coli W (ATCC 9637): comparative genome analysis and an improved genome-scale reconstruction of E. coli
    Colin T Archer
    Australian Institute for Bioengineering and Nanotechnology, Cnr Cooper and College Rds, The University of Queensland, St Lucia, Queensland 4072 Australia
    BMC Genomics 12:9. 2011

Detail Information

Publications131 found, 100 shown here

  1. doi request reprint Proteome-based identification of fusion partner for high-level extracellular production of recombinant proteins in Escherichia coli
    Zhi Gang Qian
    Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering, BioProcess Engineering Research Center, Center for Systems and Synthetic Biotechnology, KAIST, Daejeon, Republic of Korea
    Biotechnol Bioeng 101:587-601. 2008
    ..High cell density cultivation allowed production of these proteins to the levels of 250-700 mg/L in the culture medium, suggesting the good potential of this approach for the excretory production of recombinant proteins...
  2. ncbi request reprint Systems biotechnology
    Sang Yup Lee
    Department of Chemical and Biomolecular Engineering BK21 Program, BioProcess Engineering Research Center, Bioinformatics Research Center, Center for Systems and Synthetic Biotechnology, Institute for the BioCentury, KAIST, Daejeon 305 701, Korea
    Genome Inform 23:214-6. 2009
    ..It is expected that systems biotechnology will be at the heart of successful industrial biotechnology towards low carbon green growth of the world...
  3. doi request reprint Editorial: Latest methods and advances in biotechnology
    Sang Yup Lee
    Biotechnol J 9:2-4. 2014
    ..The special issue is edited by our Editors-in-Chief, Prof. Sang Yup Lee and Prof...
  4. pmc Comparative multi-omics systems analysis of Escherichia coli strains B and K-12
    Sung Ho Yoon
    Systems and Synthetic Biology Research Center, Korea Research Institute of Bioscience and Biotechnology, Yuseong, Daejeon 305 806, Korea
    Genome Biol 13:R37. 2012
    ..Here, we demonstrate that comparative analyses of multi-omics data combined with a computational modeling approach provide a framework for elucidating the phenotypic characteristics of organisms whose genomes are sequenced...
  5. pmc Framework for network modularization and Bayesian network analysis to investigate the perturbed metabolic network
    Hyun Uk Kim
    Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology KAIST, Daejeon 305 701, Republic of Korea
    BMC Syst Biol 5:S14. 2011
    ..With their increasing importance, their precise network characterization has also been crucial for better understanding of the cellular physiology...
  6. doi request reprint Editorial: Flavors of international biotechnology
    Sang Yup Lee
    Biotechnol J 8:754-5. 2013
    ..The articles in this issue showcase the diverse research in biotechnology conducted in various countries. ..
  7. pmc Genome-scale metabolic model of the fission yeast Schizosaccharomyces pombe and the reconciliation of in silico/in vivo mutant growth
    Seung Bum Sohn
    Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering BK21 Program, Center for Systems and Synthetic Biotechnology, Institute for the BioCentury, KAIST, Daejeon, Republic of Korea
    BMC Syst Biol 6:49. 2012
    ..One benchmark for measuring the in silico capability in predicting in vivo phenotypes is the use of single-gene mutant libraries to measure the accuracy of knockout simulations in predicting mutant growth phenotypes...
  8. pmc The genome-scale metabolic network analysis of Zymomonas mobilis ZM4 explains physiological features and suggests ethanol and succinic acid production strategies
    Kyung Yun Lee
    Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering BK21 Program, KAIST, 335 Gwahangno, Yuseong Gu, Daejeon 305 701, Republic of Korea
    Microb Cell Fact 9:94. 2010
    ..mobilis is required to systematically analyze in silico the metabolic characteristics of this bacterium under a wide range of genotypic and environmental conditions...
  9. pmc Genome-scale reconstruction and in silico analysis of the Ralstonia eutropha H16 for polyhydroxyalkanoate synthesis, lithoautotrophic growth, and 2-methyl citric acid production
    Jong Myoung Park
    Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering BK21 Program, KAIST, 291 Daehak ro, Yuseong Gu, Daejeon 305 701, Republic of Korea
    BMC Syst Biol 5:101. 2011
    ..Thus, it is necessary to analyze its metabolic characteristics systematically and optimize the entire metabolic network at systems level...
  10. pmc The genome sequence of E. coli W (ATCC 9637): comparative genome analysis and an improved genome-scale reconstruction of E. coli
    Colin T Archer
    Australian Institute for Bioengineering and Nanotechnology, Cnr Cooper and College Rds, The University of Queensland, St Lucia, Queensland 4072 Australia
    BMC Genomics 12:9. 2011
    ..E. coli W is a fast-growing strain and is the only safe strain that can utilize sucrose as a carbon source. Lifecycle analysis has demonstrated that sucrose from sugarcane is a preferred carbon source for industrial bioprocesses...
  11. pmc New time-scale criteria for model simplification of bio-reaction systems
    Junwon Choi
    Department of Chemical and Biomolecular Engineering BK21 Program, KAIST, 335 Gwahangro, Yuseong Gu, Daejeon, 305 701, Republic of Korea
    BMC Bioinformatics 9:338. 2008
    ..A more efficient estimation approach is necessary for complex systems...
  12. ncbi request reprint Microarrays of peptides elevated on the protein layer for efficient protein kinase assay
    Seok Jae Lee
    Metabolic and Biomolecular Engineering National Research Laboratory, BioProcess Engineering Research Center, Korea Advanced Institute of Science and Technology, 373 1 Guseong dong, Yuseong Gu, Daejeon, 305 701, Republic of Korea
    Anal Biochem 330:311-6. 2004
    ..Therefore, this new strategy will not only be useful in high-throughput and cost-effective screening of kinase substrate peptides but also be generally applicable in studying various protein-peptide interactions...
  13. ncbi request reprint Fermentative production of chemicals that can be used for polymer synthesis
    Sang Y Lee
    Department of Chemical and Biomolecular Engineering and BioProcess Engineering Research Center, Korea Advanced Institute of Science and Technology, 373 1 Guseong dong, Yuseong Gu, Daejeon 305 701, Republic of Korea
    Macromol Biosci 4:157-64. 2004
    ..In addition, the metabolic engineering and fermentation strategies for their production are reviewed...
  14. doi request reprint Integration of systems biology with bioprocess engineering: L: -threonine production by systems metabolic engineering of Escherichia coli
    Sang Yup Lee
    Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering BK21 Program, Center for Systems and Synthetic Biotechnology, Institute for the BioCentury, KAIST, 335 Gwahangno, Yuseong Gu, Daejeon, 305 701, Republic of Korea
    Adv Biochem Eng Biotechnol 120:1-19. 2010
    ..Here we review strategies and applications of systems biology successfully implemented on bioprocess engineering, with particular focus on developing L: -threonine production strains of Escherichia coli...
  15. ncbi request reprint Systems biotechnology for strain improvement
    Sang Yup Lee
    Metabolic and Biomolecular Engineering National Research Laboratory and Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology, 373 1 Guseong dong, Yuseong Gu, Daejeon 305 701, Korea
    Trends Biotechnol 23:349-58. 2005
    ..This article highlights the recent developments in these systems approaches, which we call systems biotechnology, and discusses future prospects...
  16. doi request reprint Metabolic engineering of microorganisms: general strategies and drug production
    Sang Yup Lee
    Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering, Center for Systems and Synthetic Biotechnology, Institute for the BioCentury, KAIST, Daejeon 305 701, Korea
    Drug Discov Today 14:78-88. 2009
    ..Also, systems metabolic engineering of Escherichia coli for the production of L-valine, an important drug precursor, is showcased as an important strategy of future metabolic engineering effort...
  17. doi request reprint Fermentative butanol production by Clostridia
    Sang Yup Lee
    Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering BK21 Program, Korea Advanced Institute of Science and Technology, Yuseong Gu, Daejeon 305 701, Republic of Korea
    Biotechnol Bioeng 101:209-28. 2008
    ..The strategies for strain improvement by metabolic engineering and further requirements to make fermentative butanol production a successful industrial process are also discussed...
  18. pmc Metabolic engineering of Escherichia coli for enhanced production of succinic acid, based on genome comparison and in silico gene knockout simulation
    Sang Jun Lee
    Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology, 373 1 Guseong dong, Yuseong Gu, Daejeon 305 701, Republic of Korea
    Appl Environ Microbiol 71:7880-7. 2005
    ..coli. These results suggest that the comparative genome analysis combined with in silico metabolic analysis can be an efficient way of developing strategies for strain improvement...
  19. doi request reprint From genome sequence to integrated bioprocess for succinic acid production by Mannheimia succiniciproducens
    Sang Yup Lee
    Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering BK21 Program, BioProcess Engineering Research Center, Daejeon, Republic of Korea
    Appl Microbiol Biotechnol 79:11-22. 2008
    ..Finally, we finish this mini-review by discussing the issues that need to be addressed to make this process of fermentative succinic acid production employing M. succiniciproducens to reach the industrial-scale process...
  20. pmc Display of bacterial lipase on the Escherichia coli cell surface by using FadL as an anchoring motif and use of the enzyme in enantioselective biocatalysis
    Seung Hwan Lee
    Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology, 373 1 Guseong dong, Yuseong Gu, Daejeon 305 701, Republic of Korea
    Appl Environ Microbiol 70:5074-80. 2004
    ..These results suggest that FadL may be a useful anchoring motif for displaying enzymes on the cell surface of E. coli for whole-cell biocatalysis...
  21. pmc Enhanced display of lipase on the Escherichia coli cell surface, based on transcriptome analysis
    Jong Hwan Baek
    Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering BK21 Program, BioProcess Engineering Research Center, Dongyang University, Yeongju, Gyeongbuk 750 711, Republic of Korea
    Appl Environ Microbiol 76:971-3. 2010
    ..The fused Pseudomonas fluorescens SIK W1 lipase was successfully displayed on the surface of E. coli cells, and the lipase activity could be enhanced by the coexpression of the gadBC genes identified by transcriptome analysis...
  22. doi request reprint Development of sucrose-utilizing Escherichia coli K-12 strain by cloning β-fructofuranosidases and its application for L-threonine production
    Jeong Wook Lee
    Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering BK21 Program, BioProcess Engineering Research Center, Center for Systems and Synthetic Biotechnology, and Institute for BioCentury, KAIST, Daejeon 305 701, Republic of Korea
    Appl Microbiol Biotechnol 88:905-13. 2010
    ..284 g L-threonine per g sucrose. This simple approach to make E. coli K-12 to acquire sucrose-utilizing ability and its successful biotechnological application can be employed to develop sustainable bioprocesses using renewable biomass...
  23. doi request reprint Microarray of DNA-protein complexes on poly-3-hydroxybutyrate surface for pathogen detection
    Tae Jung Park
    Department of Chemical and Biomolecular Engineering BK21 Program, Center for Systems and Synthetic Biotechnology, Institute for the BioCentury, KAIST, 335 Gwahangno, Yuseong Gu, Daejeon, 305 701, Republic of Korea
    Anal Bioanal Chem 393:1639-47. 2009
    ....
  24. pmc Label-free electrochemical diagnosis of viral antigens with genetically engineered fusion protein
    Nam Su Heo
    BioProcess Engineering Research Center, KAIST, 291 Daehak ro, Yuseong Gu, Daejeon 305 701, Korea
    Sensors (Basel) 12:10097-108. 2012
    ..14 ng/ml hbsag...
  25. pmc Prediction of novel synthetic pathways for the production of desired chemicals
    Ayoun Cho
    Department of Chemical and Biomolecular Engineering BK21 Program, KAIST, Daejeon, South Korea
    BMC Syst Biol 4:35. 2010
    ..Also, some of these methods focus on specific pathways, and thus are limited to expansion to the whole metabolism...
  26. pmc Metabolic engineering of Clostridium acetobutylicum ATCC 824 for isopropanol-butanol-ethanol fermentation
    Joungmin Lee
    Department of Chemical and Biomolecular Engineering, Institute for the BioCentury, KAIST, Daejeon, Republic of Korea
    Appl Environ Microbiol 78:1416-23. 2012
    ..The resulting PJC4BK(pIPA3-Cm2) strain produced 20.4 g/liter of total alcohol. Fermentation could be prolonged by in situ removal of solvents by gas stripping, and 35.6 g/liter of the IBE mixture could be produced in 45 h...
  27. ncbi request reprint Characterization of a bacterial self-assembly surface layer protein and its application as an electrical nanobiosensor
    Tae Jung Park
    BioProcess Engineering Research Center, Institute for the BioCentury, KAIST 335 Gwahangno, Yuseong Gu, Daejeon 305 701, Korea
    J Nanosci Nanotechnol 11:402-7. 2011
    ..Bacterial SLP could be used as a biological template for immobilization molecular array, and provides new approaches for nanoelectronic biosensor applications...
  28. pmc Construction and characterization of shuttle vectors for succinic acid-producing rumen bacteria
    Yu Sin Jang
    Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering BK21 Program and BioProcess Engineering Research Center, Republic of Korea
    Appl Environ Microbiol 73:5411-20. 2007
    ..Thus, the shuttle vectors developed in this study should be useful for genetic and metabolic engineering of succinic acid-producing rumen bacteria...
  29. ncbi request reprint Spore display using Bacillus thuringiensis exosporium protein InhA
    Tae Jung Park
    Department of Chemical and Biomolecular Engineering BK21 Program, BioProcess Engineering Research Center, Center for Systems and Synthetic Biotechnology, and Institute for the BioCentury, KAIST, Daejeon 305 701, Korea
    J Microbiol Biotechnol 19:495-501. 2009
    ..InhA-mediated anchoring of foreign proteins in the exosporium of Bacillus spores can provide a new method of microbial display, thereby broadening the potential for novel applications of microbial display...
  30. ncbi request reprint WebCell: a web-based environment for kinetic modeling and dynamic simulation of cellular networks
    Dong Yup Lee
    Department of Chemical and Biomolecular Engineering, Metabolic and Biomolecular Engineering National Research Laboratory 373 1 Guseong dong, Yuseong Gu, Daejeon 305 701, Korea
    Bioinformatics 22:1150-1. 2006
    ..A collected model library is also available to provide comprehensive implications for cellular dynamics of the published models...
  31. ncbi request reprint Micropatterning proteins on polyhydroxyalkanoate substrates by using the substrate binding domain as a fusion partner
    Jong Pil Park
    Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering, Yuseong Gu, Daejeon, Republic of Korea
    Biotechnol Bioeng 92:160-5. 2005
    ..Also, antibody binding assay by surface plasmon resonance suggested that protein-protein interaction studies could be carried out using this system...
  32. ncbi request reprint EcoProDB: the Escherichia coli protein database
    Hongseok Yun
    Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering, Center for Systems and Synthetic Biotechnology, Institute for the BioCentury, Daejeon 305 701, Korea
    Bioinformatics 23:2501-3. 2007
    ..Availability: EcoProDB database system is accessible at http://eecoli.kaist.ac.kr...
  33. doi request reprint Tailor-made type II Pseudomonas PHA synthases and their use for the biosynthesis of polylactic acid and its copolymer in recombinant Escherichia coli
    Taek Ho Yang
    Corporate R and D, LG Chem Research Park, 104 1 Moonji dong, Yuseong Gu, Daejeon 305 380, Republic of Korea
    Appl Microbiol Biotechnol 90:603-14. 2011
    ..PLA homopolymer could also be produced at ca. 7 wt.% by employing the several PhaC1 variants containing E130D/S325T/S477G/Q481K quadruple mutations in wild-type E. coli XL1-Blue...
  34. doi request reprint Genome-wide identification of the subcellular localization of the Escherichia coli B proteome using experimental and computational methods
    Mee Jung Han
    Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering BK21 Program, BioProcess Engineering Research Center, and Institute for the BioCentury, KAIST, Yuseong Gu, Daejeon, Republic of Korea
    Proteomics 11:1213-27. 2011
    ..coli B, and will be useful as a reference for proteome profiling studies under various conditions. The complete proteome data are available online (http://ecolib.kaist.ac.kr)...
  35. pmc Genome-based metabolic engineering of Mannheimia succiniciproducens for succinic acid production
    Sang Jun Lee
    Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology, 373 1 Guseong dong, Yuseong Gu, Daejeon 305 701, Republic of Korea
    Appl Environ Microbiol 72:1939-48. 2006
    ..4 g/liter of succinic acid, with a succinic acid yield of 1.16 mol succinic acid per mol glucose and a succinic acid productivity of 1.8 g/liter/h, which should be useful for industrial production of succinic acid...
  36. doi request reprint Advanced cleanup process of the free-flow microfluidic device for protein analysis
    Yun Suk Huh
    Department of Chemical and Biomolecular Engineering BK21 Program, Korea Advanced Institute of Science and Technology, Daejeon 305 701, Republic of Korea
    Ultramicroscopy 108:1365-70. 2008
    ..The inflow of the purified fusion protein sample can be successfully immobilized on the gold surface and analyzed by confocal fluorescence microscopy and surface plasmon resonance for biotechnological sensors...
  37. ncbi request reprint Display of lipase on the cell surface of Escherichia coli using OprF as an anchor and its application to enantioselective resolution in organic solvent
    Seung Hwan Lee
    Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering, and BioProcess Engineering Research Center, Korea Advanced Institute of Science and Technology, Yuseong Gu, Daejeon, Republic of Korea
    Biotechnol Bioeng 90:223-30. 2005
    ..These results suggest that E. coli cells displaying lipases using OprF as an anchoring motif can be employed for various biotechnological applications both in aqueous and nonaqueous phases...
  38. doi request reprint Programmable peptide-directed two dimensional arrays of various nanoparticles on graphene sheets
    Bong Gill Choi
    Department of Chem and Biomolecular Eng BK 21, KAIST, 291 Daehak ro, Yuseong Gu, Daejeon, 305 701, Republic of Korea
    Nanoscale 3:3208-13. 2011
    ..e., Pt, Au, Pd, and Ru), which would greatly expand the utility and versatility of this approach for the synthesis and array of the advanced nanomaterials...
  39. doi request reprint Multi-stage high cell continuous fermentation for high productivity and titer
    Ho Nam Chang
    Department of Chemical and Biomolecular Engineering, KAIST Korea Advanced Institute of Science and Technology, Daejeon, Korea
    Bioprocess Biosyst Eng 34:419-31. 2011
    ..We suggest MSC-HCDC as a new production platform for various fermentation products including monoclonal antibody...
  40. doi request reprint Microbial production of short-chain alkanes
    Yong Jun Choi
    Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering BK21 Program, BioProcess Engineering Research Center and Center for Systems and Synthetic Biotechnology, Institute for the BioCentury, Korea Advanced Institute of Science and Technology KAIST, 291 Daehak ro, Yuseong Gu, Daejeon 305 701, South Korea
    Nature 502:571-4. 2013
    ..ADP1 wax ester synthase (atfA) and the E. coli mutant alcohol dehydrogenase (adhE(mut)). ..
  41. doi request reprint Characterization of the Arc two-component signal transduction system of the capnophilic rumen bacterium Mannheimia succiniciproducens
    Won Seok Jung
    Omics and Integration Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Korea
    FEMS Microbiol Lett 284:109-19. 2008
    ..succiniciproducens ArcB. Our results indicate that different types of signaling molecules and distinct modes of kinase regulation are used by the ArcB proteins of E. coli and M. succiniciproducens...
  42. doi request reprint Development of label-free optical diagnosis for sensitive detection of influenza virus with genetically engineered fusion protein
    Tae Jung Park
    BioProcess Engineering Research Center, KAIST, 291 Daehak ro, Yuseong Gu, Daejeon 305 701, Republic of Korea
    Talanta 89:246-52. 2012
    ..It was used for the detection of influenza virus, an infectious viral disease, as an example case...
  43. doi request reprint Biosynthesis of polyhydroxyalkanoates containing 2-hydroxybutyrate from unrelated carbon source by metabolically engineered Escherichia coli
    Si Jae Park
    Chemical Biotechnology Research Center, Korea Research Institute of Chemical Technology, Yuseong Gu, Daejeon, Republic of Korea
    Appl Microbiol Biotechnol 93:273-83. 2012
    ..The metabolic engineering strategy reported here should be useful for the production of PHAs containing 2HB monomer...
  44. pmc Transcriptome and proteome analyses of adaptive responses to methyl methanesulfonate in Escherichia coli K-12 and ada mutant strains
    Jong Hwan Baek
    Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering BK21 Program, BioProcess Engineering Research Center, Center for Systems and Synthetic Biotechnology and Institute for the BioCentury, KAIST, Daejeon, Republic of Korea
    BMC Microbiol 9:186. 2009
    ..Here we present time-dependent global gene and protein expression profiles following treatment with methyl methanesulfonate (MMS) in E. coli W3110 and its ada mutant strains...
  45. ncbi request reprint Protein nanopatterns and biosensors using gold binding polypeptide as a fusion partner
    Tae Jung Park
    Metabolic and Biomolecular Engineering National Research Laboratory, BioProcess Engineering Research Center, Korea Advanced Institute of Science and Technology KAIST, Daejeon 305 701, Korea
    Anal Chem 78:7197-205. 2006
    ..Furthermore, these studies can be carried out in a microfluidic system, which allows high-throughput analysis of biomolecular interactions...
  46. doi request reprint Metabolic engineering of Escherichia coli for the production of cadaverine: a five carbon diamine
    Zhi Gang Qian
    Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering BK21 Program, BioProcess Engineering Research Center, Center for Systems and Synthetic Biotechnology, and Institute for BioCentury, KAIST, 335 Gwahangno, Yuseong Gu, Daejeon 305 701, Republic of Korea
    Biotechnol Bioeng 108:93-103. 2011
    ..61 g L(-1) of cadaverine with a productivity of 0.32 g L(-1) h(-1) by fed-batch cultivation. The strategy reported here should be useful for the bio-based production of cadaverine from renewable resources...
  47. doi request reprint Biosynthesis of enantiopure (S)-3-hydroxybutyric acid in metabolically engineered Escherichia coli
    Sang Hyun Lee
    Corporate R and D, LG Chem, Ltd Research Park, Yuseong Gu, Daejeon, Republic of Korea
    Appl Microbiol Biotechnol 79:633-41. 2008
    ..7 g l(-1), which also resulted in the decrease of the S3HB productivity to 0.05 g l(-1)h(-1)...
  48. doi request reprint Efficient production of polylactic acid and its copolymers by metabolically engineered Escherichia coli
    Yu Kyung Jung
    Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering BK21 Program, Center for Systems and Synthetic Biotechnology, Institute for the BioCentury, KAIST, Daejeon 305 701, Republic of Korea
    J Biotechnol 151:94-101. 2011
    ..coli JLXF5 strain was able to produce P(3HB-co-39.6 mol% LA) having the molecular weight of 141,000 Da to 20 g l⁻¹ with a polymer content of 43 wt% in a chemically defined medium by the pH-stat fed-batch culture...
  49. pmc Engineering Escherichia coli for increased productivity of serine-rich proteins based on proteome profiling
    Mee Jung Han
    Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering, BioProcess Engineering Research Center, Korea Advanced Institute of Science and Technology, Daejeon 305 701, Republic of Korea
    Appl Environ Microbiol 69:5772-81. 2003
    ..The approach taken in this study should be useful in designing a strategy for improving recombinant protein production...
  50. pmc Cell surface display of lipase in Pseudomonas putida KT2442 using OprF as an anchoring motif and its biocatalytic applications
    Seung Hwan Lee
    Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology, 373 1 Guseong dong, Yuseong Gu, Daejeon 305 701, Republic of Korea
    Appl Environ Microbiol 71:8581-6. 2005
    ..These results suggest that the OprF anchor can be used for efficient display of proteins in P. putida KT2442 and consequently for various biocatalytic applications...
  51. ncbi request reprint Genome-scale analysis of Mannheimia succiniciproducens metabolism
    Tae Yong Kim
    Department of Chemical and Biomolecular Engineering BK21 Program, Metabolic and Biomolecular Engineering National Research Laboratory, Korea Advanced Institute of Science and Technology, Daejeon, Republic of Korea
    Biotechnol Bioeng 97:657-71. 2007
    ..succiniciproducens to various environmental and genetic perturbations and consequently for designing rational strategies for strain improvement...
  52. doi request reprint Metabolic engineering of Escherichia coli for the production of fumaric acid
    Chan Woo Song
    Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering BK21 Program, Center for Systems and Synthetic Biotechnology, Institute for the BioCentury, Korea Advanced Institute of Science and Technology KAIST, 291 Daehak ro, Yuseong Gu, Daejeon 305 701, Republic of Korea
    Biotechnol Bioeng 110:2025-34. 2013
    ..389 g fumaric acid/g glucose and 0.448 g/L/h, respectively. This study demonstrates the possibility for the efficient production of fumaric acid by metabolically engineered E. coli...
  53. pmc Flux variability scanning based on enforced objective flux for identifying gene amplification targets
    Jong Myoung Park
    Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering BK21 Program, Center for Systems and Synthetic Biotechnology, Institute for the BioCentury, Korea Advanced Institute of Science and Technology KAIST, Daejeon, 305 701, Republic of Korea
    BMC Syst Biol 6:106. 2012
    ..Here, we report a new algorithm which incorporates physiological data into a model to improve the model's prediction capabilities and to capitalize on the relationships between genes and metabolic fluxes...
  54. doi request reprint Acetone-butanol-ethanol production with high productivity using Clostridium acetobutylicum BKM19
    Yu Sin Jang
    Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering BK21 Program, BioProcess Engineering Research Center, Center for Systems and Synthetic Biotechnology, Institute for the BioCentury, KAIST, Daejeon 305 701, Republic of Korea
    Biotechnol Bioeng 110:1646-53. 2013
    ..7 and 21.1 g L(-1)  h(-1) , and the yields of 0.17 and 0.34 g g(-1) , respectively. The obtained butanol and ABE volumetric productivities are the highest reported productivities obtained from all known-processes...
  55. ncbi request reprint Enhanced proteome profiling by inhibiting proteolysis with small heat shock proteins
    Mee Jung Han
    Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering, BioProcess Engineering Research Center, Daejeon, Republic of Korea
    J Proteome Res 4:2429-34. 2005
    ..Therefore, the use of sHsps during 2-DE significantly improves proteome profiling by generally enabling the detection of many more protein spots that could not be seen previously...
  56. doi request reprint Metabolic engineering of Escherichia coli for the production of putrescine: a four carbon diamine
    Zhi Gang Qian
    Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering BK21 Program, BioProcess Engineering Research Center, Center for Systems and Synthetic Biotechnology, Institute for the BioCentury, KAIST, 335 Gwahangno, Yuseong Gu, Daejeon 305 701, Republic of Korea
    Biotechnol Bioeng 104:651-62. 2009
    ....
  57. doi request reprint Systems metabolic engineering of microorganisms for natural and non-natural chemicals
    Jeong Wook Lee
    Department of Chemical and Biomolecular Engineering Brain Korea 21 program, Center for Systems and Synthetic Biotechnology, Institute for the BioCentury, Korea Advanced Institute of Science and Technology, Daejeon, Republic of Korea
    Nat Chem Biol 8:536-46. 2012
    ..Finally, we highlight the limitations and challenges to be overcome for the systems metabolic engineering of microorganisms at more advanced levels...
  58. doi request reprint A nanoforest structure for practical surface-enhanced Raman scattering substrates
    Myeong Lok Seol
    Department of Electrical Engineering, KAIST, Daejeon, Republic of Korea
    Nanotechnology 23:095301. 2012
    ..Simple fabrication and high throughput with strong in-plane hot-spots imply that the nanoforest structure can be a practical sensing component of a chip-based SERS sensing system...
  59. ncbi request reprint Continuous production of succinic acid using an external membrane cell recycle system
    Moon Il Kim
    Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology, Daejeon 305 701, Korea
    J Microbiol Biotechnol 19:1369-73. 2009
    ..3 times higher, respectively, compared with those obtained at the dilution rate of 0.1 h-1. These observations give a deep insight into the process design for a continuous succinic acid production by microorganisms...
  60. doi request reprint Continuous butanol production with reduced byproducts formation from glycerol by a hyper producing mutant of Clostridium pasteurianum
    Alok Malaviya
    Department of Chemical and Biomolecular Engineering BK21 Program, Institute for the BioCentury, KAIST, Yuseong Gu, Daejeon, Republic of Korea
    Appl Microbiol Biotechnol 93:1485-94. 2012
    ..9 h(-1). This study reports continuous production of butanol with reduced byproducts formation from glycerol using C. pasteurianum, and thus could help design a bioprocess for the improved production of butanol...
  61. doi request reprint Data integration and analysis of biological networks
    Tae Yong Kim
    Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering BK21 Program, Center for Systems and Synthetic Biotechnology, Institute for the BioCentury, Korea Advanced Institute of Science and Technology KAIST, Daejeon 305 701, Republic of Korea
    Curr Opin Biotechnol 21:78-84. 2010
    ..It is expected that this trend will continue, the outcomes of which will allow development of more sophisticated networks integrating diverse omics data, and enhance our understanding of biological systems...
  62. doi request reprint Proteome-based physiological analysis of the metabolically engineered succinic acid producer Mannheimia succiniciproducens LPK7
    Jeong Wook Lee
    Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering BK21 Program, BioProcess Engineering Research Center, KAIST, 335 Gwahangno, Yuseong Gu, Daejeon 305 701, Republic of Korea
    Bioprocess Biosyst Eng 33:97-107. 2010
    ....
  63. doi request reprint Site-specific immobilization of gold binding polypeptide on gold nanoparticle-coated graphene sheet for biosensor application
    Minho Yang
    Department of Chemical and Biomolecular Engineering, BK21 Program, KAIST, 291 Daehak ro, Yuseong Gu, Daejeon 305 701, Republic of Korea
    Nanoscale 3:2950-6. 2011
    ..This strategy, which integrates biotic and abiotic moieties through site-specific interactions, has a great potential for use in biosensing and bioconversion process...
  64. ncbi request reprint In silico prediction and validation of the importance of the Entner-Doudoroff pathway in poly(3-hydroxybutyrate) production by metabolically engineered Escherichia coli
    Soon Ho Hong
    Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology, 373 1 Guseong dong, Yuseong Gu, Daejeon 305 701, Republic of Korea
    Biotechnol Bioeng 83:854-63. 2003
    ..coli eda gene, which proves the important role of ED pathway on P(3HB) synthesis in recombinant E. coli as predicted by metabolic flux analysis...
  65. doi request reprint In silico analysis of the effects of H2 and CO2 on the metabolism of a capnophilic bacterium Mannheimia succiniciproducens
    Tae Yong Kim
    Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering BK21 Program, Center for Systems and Synthetic Biotechnology, Institute for the BioCentury, 335 Gwahangno, Yuseong Gu, Daejeon 305 701, Republic of Korea
    J Biotechnol 144:184-9. 2009
    ....
  66. pmc Enhanced production of insulin-like growth factor I fusion protein in Escherichia coli by coexpression of the down-regulated genes identified by transcriptome profiling
    Jong Hyun Choi
    Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering and BioProcess Engineering Research Center, Daejeon 305 701, Korea
    Appl Environ Microbiol 69:4737-42. 2003
    ..36 +/- 0.027 to 0.82 +/- 0.048 g/liter/h. These results demonstrate that transcriptome profiling can provide invaluable information in designing engineered strains showing enhanced performance...
  67. doi request reprint Detection of the most common corneal dystrophies caused by BIGH3 gene point mutations using a multispot gold-capped nanoparticle array chip
    So Young Yoo
    BioProcess Engineering Research Center, Center for Systems and Synthetic Biotechnology, Institute for the BioCentury, KAIST, 335 Gwahangno, Yuseong Gu, Daejeon 305 701, Republic of Korea
    Anal Chem 82:1349-57. 2010
    ....
  68. doi request reprint Comparison of the extracellular proteomes of Escherichia coli B and K-12 strains during high cell density cultivation
    Xiao Xia Xia
    Department of Chemical and Biomolecular Engineering, BioProcess Engineering Research Center, Institute for the BioCentury, Korea Advanced Institute of Science and Technology KAIST, Yuseong Gu, Daejeon, Republic of Korea
    Proteomics 8:2089-103. 2008
    ..These findings summarized as reference proteome maps will be useful for studying protein release in further detail, and provide new strategies for enhanced excretory production of recombinant proteins...
  69. ncbi request reprint Succinic acid production from continuous fermentation process using Mannheimia succiniciproducens LPK7
    In Jae Oh
    Department of Chemical and Biomolecular Engineering, Sogang University, Seoul 121 742, Korea
    J Microbiol Biotechnol 18:908-12. 2008
    ..To examine the quantitative balance of the metabolism, a flux distribution analysis was also performed using the metabolic network model of glycolysis and the pentose phosphate pathway...
  70. pmc Proteome-level responses of Escherichia coli to long-chain fatty acids and use of fatty acid inducible promoter in protein production
    Mee Jung Han
    Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering BK21 Program, Yuseong Gu, Daejeon 305 701, South Korea
    J Biomed Biotechnol 2008:735101. 2008
    ..These results suggest that proteomics is playing an important role not only in biological research but also in various biotechnological applications...
  71. doi request reprint Application of systems biology for bioprocess development
    Jin Hwan Park
    Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering, Center for Systems and Synthetic Biotechnology, Institute for the BioCentury, KAIST, Daejeon 305 701, Korea
    Trends Biotechnol 26:404-12. 2008
    ..Here, we review the applications of systems biology for the development of strains and bioprocesses by means of several successful examples and, furthermore, discuss future prospects...
  72. ncbi request reprint Development of a markerless gene knock-out system for Mannheimia succiniciproducens using a temperature-sensitive plasmid
    Ji Mahn Kim
    Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering BK21 Program, BioProcess Engineering Research Center, Institute for the BioCentury, Daejeon, Korea
    FEMS Microbiol Lett 278:78-85. 2008
    ..This markerless deletion method should be useful for further metabolic engineering of M. succiniciproducens, which is a promising industrial bacterium for succinic acid production from renewable resources...
  73. doi request reprint Single-step multiplex detection of toxic metal ions by Au nanowires-on-chip sensor using reporter elimination
    Taejoon Kang
    Department of Chemistry, KAIST, Daejeon 305 701, Korea
    Lab Chip 12:3077-81. 2012
    ..We successfully detected ions of mercury (Hg(2+)), silver (Ag(+)), and lead (Pb(2+)) coexisting in the same solution by using this sensor...
  74. ncbi request reprint Biosynthesis of R-3-hydroxyalkanoic acids by metabolically engineered Escherichia coli
    Si Jae Park
    Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering, BioProcess Engineering Research Center, Republic of Korea
    Appl Biochem Biotechnol 113:373-9. 2004
    ..When the R. eutropha intracellular depolymerase gene was expressed by using a stable plasmid containing the hok/sok locus of plasmid R1, R3HB could be efficiently produced...
  75. doi request reprint Genome-scale metabolic model of methylotrophic yeast Pichia pastoris and its use for in silico analysis of heterologous protein production
    Seung Bum Sohn
    Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering BK21 Program, Center for Systems and Synthetic Biotechnology, Institute for the BioCentury, KAIST, 335 Gwahangno, Daejeon, Republic of Korea
    Biotechnol J 5:705-15. 2010
    ..The protein-producing model versions of PpaMBEL1254 were then analyzed to examine the impact on oxygen limitation on protein production...
  76. ncbi request reprint Alignment of SWNTs by protein-ligand interaction of functionalized magnetic particles under low magnetic fields
    Tae Jung Park
    BioProcess Engineering Research Center, Institute for the BioCentury, KAIST 335 Gwahangno, Yuseong Gu, Daejeon 305 701, Korea
    J Nanosci Nanotechnol 11:4540-5. 2011
    ..We suggest that this approach, which consists of a combination of biological interaction among streptavidin-biotin and magnetite particles, should be useful for lateral orientation of individual SWNTs with controllable direction...
  77. doi request reprint Plasmonic properties of the multispot copper-capped nanoparticle array chip and its application to optical biosensors for pathogen detection of multiplex DNAs
    Do Kyun Kim
    BioProcess Engineering Research Center, Center for Systems and Synthetic Biotechnology, KAIST, Yuseong Gu, Daejeon, Republic of Korea
    Anal Chem 83:6215-22. 2011
    ..The optical biosensor developed in this study represents a unique approach to performing LSPR that utilizes a simple and cost-effective optical setup with disposable chips...
  78. pmc Prediction of metabolic fluxes by incorporating genomic context and flux-converging pattern analyses
    Jong Myoung Park
    Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering, KAIST, Daejeon 305 701, Republic of Korea
    Proc Natl Acad Sci U S A 107:14931-6. 2010
    ..Thus, this strategy will be useful for accurately predicting the intracellular fluxes of large metabolic networks when their experimental determination is difficult...
  79. doi request reprint Fed-batch culture of Escherichia coli for L-valine production based on in silico flux response analysis
    Jin Hwan Park
    Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering, BK21 Program, KAIST, Daejeon 305 701, Republic of Korea
    Biotechnol Bioeng 108:934-46. 2011
    ..coli. This approach of employing systems-level analysis of metabolic fluxes in developing fed-batch cultivation strategy would also be applicable in developing strategies for the efficient production of other bioproducts...
  80. doi request reprint Biosynthesis of polylactic acid and its copolymers using evolved propionate CoA transferase and PHA synthase
    Taek Ho Yang
    Corporate R and D, LG Chem Research Park, 104 1 Moonji dong, Yuseong Gu, Daejeon 305 380, Republic of Korea
    Biotechnol Bioeng 105:150-60. 2010
    ..Fed-batch cultures were performed to produce P(3HB-co-LA) copolymers having 9-64 mol% of lactate, and their molecular weights, thermal properties, and melt flow properties were determined...
  81. ncbi request reprint The proteome of Mannheimia succiniciproducens, a capnophilic rumen bacterium
    Jeong Wook Lee
    Department of Chemical and Biomolecular Engineering, Metabolic and Biomolecular Engineering National Research Laboratory, BioProcess Engineering Research Center, Korea Advanced Institute of Science and Technology, Daejeon, Republic of Korea
    Proteomics 6:3550-66. 2006
    ..Comparative proteome profiling revealed valuable information to understand physiological changes during growth, and subsequently suggested target genes to be manipulated for the strain improvement...
  82. doi request reprint Development of a fully integrated microfluidic system for sensing infectious viral disease
    Yun Suk Huh
    Department of Chemical and Biomolecular Engineering BK21 Program, KAIST, Yuseong Gu, Daejeon, Korea
    Electrophoresis 29:2960-9. 2008
    ..It was used for the detection of severe acute respiratory syndrome (SARS), an infectious viral disease, as an example case...
  83. ncbi request reprint Optimization and scale-up of succinic acid production by Mannheimia succiniciproducens LPK7
    In Jae Oh
    Department of Chemical and Biomolecular Engineering, Sogang University, Seoul 121 742, Korea
    J Microbiol Biotechnol 19:167-71. 2009
    ..4 g/l and 0.86 g/g. This result shows similar amount of succinic acid obtained in lab-scale fermentation, and it is possible to scale up to larger fermentors without major problems...
  84. pmc Systems metabolic engineering of Escherichia coli for L-threonine production
    Kwang Ho Lee
    Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering BK21 Program, BioProcess Engineering Research Center, Korea Advanced Institute of Science and Technology KAIST, Daejeon, Korea
    Mol Syst Biol 3:149. 2007
    ..4 g/l Thr by fed-batch culture. The systems metabolic engineering strategy reported here may be broadly employed for developing genetically defined organisms for the efficient production of various bioproducts...
  85. ncbi request reprint Knowledge representation model for systems-level analysis of signal transduction networks
    Dong Yup Lee
    Department of Chemical and Biomolecular Engineering and Bioinformatics Research Center, Korea Advanced Institute of Science and Technology, 373 1 Guseong dong, Yuseong Gu, Daejeon 305 701, Republic of Korea
    Genome Inform 15:234-43. 2004
    ..This strategy should be useful in drug target discovery and its validation...
  86. ncbi request reprint MetaFluxNet, a program package for metabolic pathway construction and analysis, and its use in large-scale metabolic flux analysis of Escherichia coli
    Sang Yup Lee
    Metabolic and Biomolecular Engineering National Research Laboratory, Korea Advanced Institute of Science and Technology, 373 1 Guseong dong, Yuseong Gu, Daejeon 305 701, Republic of Korea
    Genome Inform 14:23-33. 2003
    ..The results indicated that among three carbon sources available, the most reduced substrate is sorbitol which yields efficient succinic acid production. The software can be downloaded from http://mbel.kaist.ac.kr/...
  87. doi request reprint Label-free optical diagnosis of hepatitis B virus with genetically engineered fusion proteins
    Shun Zheng
    Department of Chemical and Biomolecular Engineering BK21 Program, KAIST, Yuseong Gu, Daejeon, Republic of Korea
    Talanta 82:803-9. 2010
    ....
  88. doi request reprint Development of chemically defined medium for Mannheimia succiniciproducens based on its genome sequence
    Hyohak Song
    Department of Chemical and Biomolecular Engineering BK21 Program, KAIST, 335 Gwahangno, Yuseong Gu, Daejeon 305 701, Republic of Korea
    Appl Microbiol Biotechnol 79:263-72. 2008
    ..The strategy reported in this paper should be generally applicable to the development of CDMs for other organisms, whose genome sequences are available...
  89. doi request reprint Development of a whole-cell biosensor by cell surface display of a gold-binding polypeptide on the gold surface
    Tae Jung Park
    BioProcess Engineering Research Center, Center for Systems and Synthetic Biotechnology, Institute for the BioCentury, KAIST, Daejeon, Korea
    FEMS Microbiol Lett 293:141-7. 2009
    ....
  90. ncbi request reprint Constitutive production of human leptin by fed-batch culture of recombinant rpoS- Escherichia coli
    Ki Jun Jeong
    Department of Chemical and Biomolecular Engineering and BioProcess Engineering Research Center, Korea Advanced Institute of Science and Technology, 373 1 Guseong dong, Yuseong Gu, Daejeon 305 701, Republic of Korea
    Protein Expr Purif 36:150-6. 2004
    ..These results suggest that the HCE promoter can be used for the efficient production of leptin, and most likely other recombinant proteins, in a constitutive manner...
  91. doi request reprint Microbial small heat shock proteins and their use in biotechnology
    Mee Jung Han
    Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology KAIST, Yuseong Gu, Daejeon 305 701, Republic of Korea
    Biotechnol Adv 26:591-609. 2008
    ..In this review, we discuss the properties and diversity of microbial sHsps, as well as their potential uses in the biotechnology industry...
  92. doi request reprint In silico genome-scale metabolic analysis of Pseudomonas putida KT2440 for polyhydroxyalkanoate synthesis, degradation of aromatics and anaerobic survival
    Seung Bum Sohn
    Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering BK21 Program, Center for Systems and Synthetic Biotechnology, Institute for the BioCentury, KAIST, Daejeon, Republic of Korea
    Biotechnol J 5:739-50. 2010
    ..Through analysis of PpuMBEL1071, extended survival of P. putida under anaerobic stress was achieved by introducing the ackA gene from Pseudomonas aeruginosa and Escherichia coli...
  93. ncbi request reprint Roles and applications of small heat shock proteins in the production of recombinant proteins in Escherichia coli
    Mee Jung Han
    Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering and BioProcess Engineering Research Center, Korea Advanced Institute of Science and Technology, Daejeon, Republic of Korea
    Biotechnol Bioeng 88:426-36. 2004
    ..This strategy seems to be generally applicable as it was successfully employed for the enhanced cytosolic or secretory production of several other recombinant proteins in E. coli...
  94. doi request reprint Patterned multiplex pathogen DNA detection by Au particle-on-wire SERS sensor
    Taejoon Kang
    Department of Chemistry, KAIST, Daejeon, Korea
    Nano Lett 10:1189-93. 2010
    ..Target DNAs from reference bacteria and clinical isolates were successfully identified by this sensor system, enabling diagnostics for infectious diseases...
  95. ncbi request reprint Combined transcriptome and proteome analysis of Escherichia coli during high cell density culture
    Sung Ho Yoon
    Department of Chemical and Biomolecular Engineering, and BioProcess Engineering Research Center, Korea Advanced Institute of Science and Technology, 373 1 Guseong dong, Yuseong Gu, Daejeon 305 701, Korea
    Biotechnol Bioeng 81:753-67. 2003
    ..These findings should be invaluable in designing metabolic engineering and fermentation strategies for the production of recombinant proteins and metabolites by HCDC of E. coli...
  96. pmc Excretion of human beta-endorphin into culture medium by using outer membrane protein F as a fusion partner in recombinant Escherichia coli
    Ki Jun Jeong
    Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering and BioProcess Engineering Research Center, Korea Advanced Institute of Science and Technology, Yuseong Gu, Daejeon 305 701, Korea
    Appl Environ Microbiol 68:4979-85. 2002
    ..From 2.7 liters of culture supernatant, 545.4 mg of beta-endorphin was obtained...
  97. doi request reprint Construction and optimization of synthetic pathways in metabolic engineering
    DoKyun Na
    Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering BK21 Program, Korea Advanced Institute of Science and Technology, Daejeon 305 701, Republic of Korea
    Curr Opin Microbiol 13:363-70. 2010
    ....
  98. ncbi request reprint Biosynthesis of poly(3-hydroxybutyrate- co-3-hydroxyalkanoates) by metabolically engineered Escherichia coli strains
    Si Jae Park
    Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering, BioProcess Engineering Research Center, Republic of Korea
    Appl Biochem Biotechnol 113:335-46. 2004
    ..coli strains constructed in this study should be useful for the production of PHAs suitable for various commercial applications...
  99. pmc Metabolic engineering of Escherichia coli for production of enantiomerically pure (R)-(--)-hydroxycarboxylic acids
    Sang Yup Lee
    Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology, Yuseong Gu, Daejeon 305 701, Korea
    Appl Environ Microbiol 69:3421-6. 2003
    ..This strategy can be used for the production of various enantiomerically pure (R)-hydroxycarboxylic acids from renewable resources...
  100. ncbi request reprint Microbial cell-surface display
    Sang Yup Lee
    Metabolic and Biomolecular Engineering National Research Laboratory, Daejeon, South Korea
    Trends Biotechnol 21:45-52. 2003
    ..Microbial cell-surface display has many potential applications, including live vaccine development, peptide library screening, bioconversion using whole cell biocatalyst and bioadsorption...
  101. ncbi request reprint In silico metabolic pathway analysis and design: succinic acid production by metabolically engineered Escherichia coli as an example
    Sang Yup Lee
    Department of Chemical and Biomolecular Engineering and BioProcess Engineering Research Center, Korea Advanced Institute of Science and Technology, Daejeon, Republic of Korea
    Genome Inform 13:214-23. 2002
    ..The results of wet experiments indicate that the pathway predicted by in silico analysis is more efficient than conventional pathway...