Innate immune function by Toll-like receptors: distinct responses in newborns and the elderlyTobias R Kollmann
Division of Infectious and Immunological Diseases, Department of Pediatrics, University of British Columbia, Vancouver, BC, Canada
Immunity 37:771-83. 2012
..The near-mirror-image patterns that emerge in contrasts of early versus late innate immune ontogeny emphasize changes in host-environment interactions as the underlying molecular and teleologic drivers...
Neonatal innate TLR-mediated responses are distinct from those of adultsTobias R Kollmann
Division of Infectious and Immunological Diseases, Department of Pediatrics, University of British Columbia, CFRI, 950 West 28th Avenue, Vancouver, BC, V5Z4H4, Canada
J Immunol 183:7150-60. 2009
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- Neonatal immunization with Listeria monocytogenes induces T cells with an adult-like avidity, sensitivity, and TCR-Vbeta repertoire, and does not adversely impact the response to boosting
Kinga K Smolen
Department of Experimental Medicine, Faculty of Medicine, University of British Columbia, Vancouver, British Columbia, Canada
Vaccine 28:235-42. 2009
..Overall, our data provide further evidence in support of immunization at birth as a feasible public health strategy to combat early life infections...
- A single immunization near birth elicits immediate and lifelong protective immunity
Brian A Reikie
Department of Medicine, University of British Columbia, Vancouver, British Columbia, V5Z 1M9, Canada
Vaccine 29:83-90. 2010
..This is the first direct evidence that vaccination--even in the absence of a booster dose--is capable of inducing immediate and lifelong protective immune memory regardless of age at the time of initial vaccination...
- Antibody responses to vaccination among South African HIV-exposed and unexposed uninfected infants during the first 2 years of life
Brian A Reikie
Division of Infectious and Immunological Diseases and Centre for Understanding and Preventing Infections in Children, Department of Pediatrics, University of British Columbia, Vancouver, British Columbia, Canada
Clin Vaccine Immunol 20:33-8. 2013
..However, our data also suggest that a large fraction of both HEU and HUU South African infants have antibody titers for several infectious threats that remain below the level of protection for much of their first 2 years of life...
- Ontogeny of Toll-like receptor mediated cytokine responses of human blood mononuclear cells
Nathan P Corbett
Division of Infectious and Immunological Diseases, Department of Pediatrics, University of British Columbia, Vancouver, Canada
PLoS ONE 5:e15041. 2010
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- Identification of B cells through negative gating-An example of the MIFlowCyt standard applied
Darren Blimkie
Department of Pediatrics, Division of Infectious and Immunological Diseases, Child and Family Research Institute, University of British Columbia, Vancouver, British Columbia, Canada
Cytometry A 77:546-51. 2010
..We demonstrate how a MIFlowCyt-compliant report can be prepared with minimal effort, and yet provide the reader with a much clearer picture of the portrayed FCM experiment and data...
- Variables to be controlled in the assessment of blood innate immune responses to Toll-like receptor stimulation
Darren Blimkie
Division of Infectious and Immunological Diseases, Department of Pediatrics, University of British Columbia, Vancouver, BC, Canada
J Immunol Methods 366:89-99. 2011
..In summary, for innate immune analysis scrupulous adherence to standard operating procedures is paramount...
- Age of recipient and number of doses differentially impact human B and T cell immune memory responses to HPV vaccination
Kinga K Smolen
Experimental Medicine Program, Department of Medicine, University of British Columbia, Vancouver, BC, Canada
Vaccine 30:3572-9. 2012
..Our data indicate that rational design of vaccines has to be tailored according to the desired induction of B and/or T cell memory...
- Correlation analysis of intracellular and secreted cytokines via the generalized integrated mean fluorescence intensity
Parisa Shooshtari
Terry Fox Laboratory, British Columbia Cancer Agency, Vancouver, BC, Canada
Cytometry A 77:873-80. 2010
..We refer to this model as generalized iMFI...
- Ontogeny of Toll-like receptor mediated cytokine responses of South African infants throughout the first year of life
Brian A Reikie
Division of Infectious and Immunological Diseases, Department of Pediatrics, Centre for Understanding and Preventing Infections in Children, University of British Columbia, Vancouver, British Colombia, Canada
PLoS ONE 7:e44763. 2012
..This is the first report depicting dramatic differences in innate immune ontogeny between different populations in the world, with important implications for global vaccination strategies...
- GLA-SE, a synthetic toll-like receptor 4 agonist, enhances T-cell responses to influenza vaccine in older adults
Hayedeh Behzad
Vancouver Coastal Health Research Institute, Vancouver, Canada
J Infect Dis 205:466-73. 2012
..The decline in influenza vaccine efficacy in older adults is associated with a limited ability of current split-virus vaccines (SVVs) to stimulate cytotoxic T lymphocyte (CTL) responses required for clinical protection against influenza...
- Flow cytometry data standards
Josef Spidlen
Terry Fox Laboratory, BC Cancer Agency, Vancouver, BC, Canada
BMC Res Notes 4:50. 2011
..abstract:..
- Systems biology evaluation of immune responses induced by human host defence peptide LL-37 in mononuclear cells
Neeloffer Mookherjee
Centre for Microbial Diseases and Immunity Research, University of British Columbia, Vancouver, BC, Canada
Mol Biosyst 5:483-96. 2009
..These data are discussed as reflecting the integration of several responsive signalling pathways through the involvement of transcription factor complexes in gene expression activated by LL-37 in human mononuclear cells...
- Profound lack of interleukin (IL)-12/IL-23p40 in neonates born early in gestation is associated with an increased risk of sepsis
Pascal M Lavoie
Child and Family Research Institute, Vancouver, British Columbia, Canada
J Infect Dis 202:1754-63. 2010
..Infants born prematurely are highly vulnerable to infections and also exhibit a high susceptibility to organ damage due to inflammation...
- Fine-tuning the safety and immunogenicity of Listeria monocytogenes-based neonatal vaccine platforms
Daniela I M Loeffler
Department of Paediatrics, Child and Family Research Institute, Vancouver, BC, Canada
Vaccine 27:919-27. 2009
..Our phagosomal driven Lm-based vaccine platform presents the broadest (cellular & humoral response) and most efficient (highly protective) vaccine platform for neonatal vaccination yet described...
