David J King

Summary

Publications

  1. ncbi request reprint Mammalian cell display and somatic hypermutation in vitro for human antibody discovery
    David J King
    Anaptysbio Inc, 10421 Pacific Center Court, San Diego, CA 92121, USA
    Curr Drug Discov Technol 11:56-64. 2014
  2. pmc Humanization of antibodies using heavy chain complementarity-determining region 3 grafting coupled with in vitro somatic hypermutation
    Peter M Bowers
    Anaptysbio Inc, San Diego, California 92131, USA
    J Biol Chem 288:7688-96. 2013
  3. pmc Simultaneous surface display and secretion of proteins from mammalian cells facilitate efficient in vitro selection and maturation of antibodies
    Robert A Horlick
    AnaptysBio, Inc, San Diego, California 92121, USA
    J Biol Chem 288:19861-9. 2013
  4. doi request reprint Mammalian cell display for the discovery and optimization of antibody therapeutics
    Peter M Bowers
    Anaptysbio Inc, 10421 Pacific Center Court, San Diego, CA 92121, USA
    Methods 65:44-56. 2014
  5. doi request reprint An integrated approach to extreme thermostabilization and affinity maturation of an antibody
    Audrey D McConnell
    AnaptysBio, Inc, 10421 Pacific Center Court, Suite 200, San Diego, CA 92121, USA
    Protein Eng Des Sel 26:151-64. 2013
  6. pmc High affinity humanized antibodies without making hybridomas; immunization paired with mammalian cell display and in vitro somatic hypermutation
    Audrey D McConnell
    AnaptysBio, Inc, San Diego, California, USA
    PLoS ONE 7:e49458. 2012
  7. pmc Coupling mammalian cell surface display with somatic hypermutation for the discovery and maturation of human antibodies
    Peter M Bowers
    AnaptysBio, Inc, 10421 Pacific Center Court, Suite 200, San Diego, CA 92121, USA
    Proc Natl Acad Sci U S A 108:20455-60. 2011

Collaborators

  • Peter M Bowers
  • Robert A Horlick
  • Audrey D McConnell
  • John L Macomber
  • Tamlyn Y Neben
  • Irina P Krapf
  • Geoffery L Tomlinson
  • Petra Verdino
  • Betty Chau
  • Betty F Wu
  • Xue Zhang
  • Jennifer L Dalton
  • Ashley D Berkebile
  • Larry Altobell
  • Rachelle M Toobian
  • Andy Chen
  • Laurence Altobell
  • Eric Hare
  • Marilyn R Kehry
  • Steven Neben
  • Joseph C Sheffer
  • Ashley Berkebile
  • Vladimir Spasojevich
  • Minjee Do
  • Vladimir Spasojevic
  • Andy P Chen
  • Josh Maclaren
  • William J Boyle
  • Trevin Holland
  • Heather A Jones
  • Steven S Neben
  • Audrey McConnell
  • Geoffrey L Tomlinson

Detail Information

Publications7

  1. ncbi request reprint Mammalian cell display and somatic hypermutation in vitro for human antibody discovery
    David J King
    Anaptysbio Inc, 10421 Pacific Center Court, San Diego, CA 92121, USA
    Curr Drug Discov Technol 11:56-64. 2014
    ....
  2. pmc Humanization of antibodies using heavy chain complementarity-determining region 3 grafting coupled with in vitro somatic hypermutation
    Peter M Bowers
    Anaptysbio Inc, San Diego, California 92131, USA
    J Biol Chem 288:7688-96. 2013
    ..The resulting antibody contained half the heavy chain murine donor sequence compared with the same antibody humanized using traditional methods...
  3. pmc Simultaneous surface display and secretion of proteins from mammalian cells facilitate efficient in vitro selection and maturation of antibodies
    Robert A Horlick
    AnaptysBio, Inc, San Diego, California 92121, USA
    J Biol Chem 288:19861-9. 2013
    ..This system is particularly useful in the context of in situ mutagenesis, as in the case of in vitro somatic hypermutation. ..
  4. doi request reprint Mammalian cell display for the discovery and optimization of antibody therapeutics
    Peter M Bowers
    Anaptysbio Inc, 10421 Pacific Center Court, San Diego, CA 92121, USA
    Methods 65:44-56. 2014
    ..This approach overcomes many of the previous limitations of mammalian cell display, enabling direct selection and maturation of antibodies as full-length, glycosylated IgGs. ..
  5. doi request reprint An integrated approach to extreme thermostabilization and affinity maturation of an antibody
    Audrey D McConnell
    AnaptysBio, Inc, 10421 Pacific Center Court, Suite 200, San Diego, CA 92121, USA
    Protein Eng Des Sel 26:151-64. 2013
    ....
  6. pmc High affinity humanized antibodies without making hybridomas; immunization paired with mammalian cell display and in vitro somatic hypermutation
    Audrey D McConnell
    AnaptysBio, Inc, San Diego, California, USA
    PLoS ONE 7:e49458. 2012
    ..This approach enables the efficient sampling of an immune repertoire and the direct selection and maturation of high-affinity, humanized IgGs...
  7. pmc Coupling mammalian cell surface display with somatic hypermutation for the discovery and maturation of human antibodies
    Peter M Bowers
    AnaptysBio, Inc, 10421 Pacific Center Court, Suite 200, San Diego, CA 92121, USA
    Proc Natl Acad Sci U S A 108:20455-60. 2011
    ..This approach overcomes many of the previous limitations of mammalian cell display, enabling direct selection and maturation of antibodies as full-length, glycosylated IgGs...