Douglas S Walsh

Summary

Affiliation: The US Army Medical Research Unit
Country: Kenya

Publications

  1. pmc Antimicrobial resistance surveillance in the AFHSC-GEIS network
    William G Meyer
    Armed Forces Health Surveillance Center, 11800 Tech Rd, Silver Spring, MD 20904, USA
    BMC Public Health 11:S8. 2011
  2. ncbi request reprint Buruli ulcer (Mycobacterium ulcerans infection)
    Douglas S Walsh
    United States Army Medical Research Unit Kenya, Walter Reed Project, PO Box 54, Kisumu, 10400, Kenya
    Trans R Soc Trop Med Hyg 102:969-78. 2008
  3. ncbi request reprint Clinical and histological features of inoculation site skin lesions in cynomolgus monkeys experimentally infected with Orientia tsutsugamushi
    Douglas S Walsh
    Department of Immunology and Medicine, United States Army Medical Component, Armed Forces Research Institute of Medical Sciences AFRIMS, Bangkok, Thailand
    Vector Borne Zoonotic Dis 7:547-54. 2007
  4. ncbi request reprint Clinical and histologic features of skin lesions in a cynomolgus monkey experimentally infected with mycobacterium ulcerans (Buruli ulcer) by intradermal inoculation
    Douglas S Walsh
    Department of Immunology and Medicine, US Army Medical Component, Armed Forces Research Institute of Medical Sciences AFRIMS, Bangkok, Thailand
    Am J Trop Med Hyg 76:132-4. 2007
  5. pmc Direct comparison of the histidine-rich protein-2 enzyme-linked immunosorbent assay (HRP-2 ELISA) and malaria SYBR green I fluorescence (MSF) drug sensitivity tests in Plasmodium falciparum reference clones and fresh ex vivo field isolates from Cambodia
    Suwanna Chaorattanakawee
    Department of Immunology and Medicine, Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand
    Malar J 12:239. 2013
  6. pmc Optimizing the HRP-2 in vitro malaria drug susceptibility assay using a reference clone to improve comparisons of Plasmodium falciparum field isolates
    Wiriya Rutvisuttinunt
    Department of Immunology and Medicine, US Army Medical Corps, Armed Forces Research Institute of Medical Sciences USAMC AFRIMS, Bangkok, Thailand
    Malar J 11:325. 2012
  7. pmc Efficacy of two versus three-day regimens of dihydroartemisinin-piperaquine for uncomplicated malaria in military personnel in northern Cambodia: an open-label randomized trial
    Chanthap Lon
    US Army Medical Component, Armed Forces Research Institute of Medical Sciences, Department of Immunology and Medicine, Bangkok, Thailand US Army Medical Component, Armed Forces Research Institute of Medical Sciences, Phnom Penh, Cambodia
    PLoS ONE 9:e93138. 2014
  8. pmc Severe malaria in Battambang Referral Hospital, an area of multidrug resistance in Western-Cambodia: a retrospective analysis of cases from 2006-2009
    Chanthap Lon
    Department of Immunology and Medicine, Armed Forces Research Institute of Medical Sciences, 315 6 Rajvithi Road, Bangkok 10400, Thailand
    Malar J 12:217. 2013
  9. pmc Pre-existing cross-reactive antibodies to avian influenza H5N1 and 2009 pandemic H1N1 in US military personnel
    Sathit Pichyangkul
    Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand National Center for Genetic Engineering and Biotechnology, Pathum Thani, Thailand Department of Microbiology, Faculty of Science, Mahidol University, Bangkok, Thailand Department of Dentistry, Chulalongkorn University, Bangkok, Thailand Hong Kong University Pasteur Research Center, Hong Kong
    Am J Trop Med Hyg 90:149-52. 2014
  10. pmc Ex vivo drug sensitivity profiles of Plasmodium falciparum field isolates from Cambodia and Thailand, 2005 to 2010, determined by a histidine-rich protein-2 assay
    Stuart D Tyner
    Department of Immunology and Medicine, US Army Medical Corps, Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand
    Malar J 11:198. 2012

Collaborators

Detail Information

Publications24

  1. pmc Antimicrobial resistance surveillance in the AFHSC-GEIS network
    William G Meyer
    Armed Forces Health Surveillance Center, 11800 Tech Rd, Silver Spring, MD 20904, USA
    BMC Public Health 11:S8. 2011
    ..This section documents the accomplishments and activities of the network through AFHSC-GEIS partners in 2009...
  2. ncbi request reprint Buruli ulcer (Mycobacterium ulcerans infection)
    Douglas S Walsh
    United States Army Medical Research Unit Kenya, Walter Reed Project, PO Box 54, Kisumu, 10400, Kenya
    Trans R Soc Trop Med Hyg 102:969-78. 2008
    ..The first cultivation of M. ulcerans from nature was reported in 2008...
  3. ncbi request reprint Clinical and histological features of inoculation site skin lesions in cynomolgus monkeys experimentally infected with Orientia tsutsugamushi
    Douglas S Walsh
    Department of Immunology and Medicine, United States Army Medical Component, Armed Forces Research Institute of Medical Sciences AFRIMS, Bangkok, Thailand
    Vector Borne Zoonotic Dis 7:547-54. 2007
    ..This pilot study shows that cynomolgus intradermally inoculated with O. tsutsugamushi replicate the localized cutaneous pathogenesis of human scrub typhus infections, strengthening the value of this animal model...
  4. ncbi request reprint Clinical and histologic features of skin lesions in a cynomolgus monkey experimentally infected with mycobacterium ulcerans (Buruli ulcer) by intradermal inoculation
    Douglas S Walsh
    Department of Immunology and Medicine, US Army Medical Component, Armed Forces Research Institute of Medical Sciences AFRIMS, Bangkok, Thailand
    Am J Trop Med Hyg 76:132-4. 2007
    ..This report, the first to describe a non-human primate experimentally infected with M. ulcerans, suggests that cynomolgus monkeys are modestly susceptible and develop some of the clinical and histologic features of Buruli ulcer...
  5. pmc Direct comparison of the histidine-rich protein-2 enzyme-linked immunosorbent assay (HRP-2 ELISA) and malaria SYBR green I fluorescence (MSF) drug sensitivity tests in Plasmodium falciparum reference clones and fresh ex vivo field isolates from Cambodia
    Suwanna Chaorattanakawee
    Department of Immunology and Medicine, Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand
    Malar J 12:239. 2013
    ....
  6. pmc Optimizing the HRP-2 in vitro malaria drug susceptibility assay using a reference clone to improve comparisons of Plasmodium falciparum field isolates
    Wiriya Rutvisuttinunt
    Department of Immunology and Medicine, US Army Medical Corps, Armed Forces Research Institute of Medical Sciences USAMC AFRIMS, Bangkok, Thailand
    Malar J 11:325. 2012
    ..Although in vitro anti-malarial susceptibility tests are widely used, uncertainties remain regarding interpretation of P. falciparum field isolate values...
  7. pmc Efficacy of two versus three-day regimens of dihydroartemisinin-piperaquine for uncomplicated malaria in military personnel in northern Cambodia: an open-label randomized trial
    Chanthap Lon
    US Army Medical Component, Armed Forces Research Institute of Medical Sciences, Department of Immunology and Medicine, Bangkok, Thailand US Army Medical Component, Armed Forces Research Institute of Medical Sciences, Phnom Penh, Cambodia
    PLoS ONE 9:e93138. 2014
    ..We compared two regimens of dihydroartemisinin-piperaquine in military and civilians on the Thai-Cambodian border to evaluate national treatment policy...
  8. pmc Severe malaria in Battambang Referral Hospital, an area of multidrug resistance in Western-Cambodia: a retrospective analysis of cases from 2006-2009
    Chanthap Lon
    Department of Immunology and Medicine, Armed Forces Research Institute of Medical Sciences, 315 6 Rajvithi Road, Bangkok 10400, Thailand
    Malar J 12:217. 2013
    ..Little has been reported in the peer-reviewed literature regarding the burden of severe malaria (SM) in Cambodia...
  9. pmc Pre-existing cross-reactive antibodies to avian influenza H5N1 and 2009 pandemic H1N1 in US military personnel
    Sathit Pichyangkul
    Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand National Center for Genetic Engineering and Biotechnology, Pathum Thani, Thailand Department of Microbiology, Faculty of Science, Mahidol University, Bangkok, Thailand Department of Dentistry, Chulalongkorn University, Bangkok, Thailand Hong Kong University Pasteur Research Center, Hong Kong
    Am J Trop Med Hyg 90:149-52. 2014
    ..Although unclear if the result of prior vaccination or disease exposure, these pre-existing antibodies may prevent or reduce disease severity...
  10. pmc Ex vivo drug sensitivity profiles of Plasmodium falciparum field isolates from Cambodia and Thailand, 2005 to 2010, determined by a histidine-rich protein-2 assay
    Stuart D Tyner
    Department of Immunology and Medicine, US Army Medical Corps, Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand
    Malar J 11:198. 2012
    ....
  11. pmc Evaluation of parasite subpopulations and genetic diversity of the msp1, msp2 and glurp genes during and following artesunate monotherapy treatment of Plasmodium falciparum malaria in Western Cambodia
    Panita Gosi
    Department of Immunology and Medicine, Armed Forces Research Institute of Medical Science AFRIMS, Bangkok, Thailand
    Malar J 12:403. 2013
    ..An additional objective was to investigate whether the classification of clinical outcomes remained valid when additional genotyping was performed...
  12. pmc Limited susceptibility of cynomolgus monkeys (Macaca fascicularis) to leprosy after experimental administration of Mycobacterium leprae
    Gerald P Walsh
    Leonard Wood Memorial, Cebu Leprosy and Tuberculosis Research Foundation, Cebu, Philippines
    Am J Trop Med Hyg 87:327-36. 2012
    ..Overall, however, cynomolgus monkeys seem minimally susceptible to leprosy after experimental M. leprae administration...
  13. doi request reprint Buruli ulcer: Advances in understanding Mycobacterium ulcerans infection
    Douglas S Walsh
    Department of Immunology and Medicine, Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand
    Dermatol Clin 29:1-8. 2011
    ..First-line therapy for BU is rifampin plus streptomycin, sometimes with surgery. New insights into the pathogenesis of BU should improve control strategies...
  14. ncbi request reprint Recent advances in leprosy and Buruli ulcer (Mycobacterium ulcerans infection)
    Douglas S Walsh
    Department of Immunology and Medicine, United States Army Medical Component, Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand
    Curr Opin Infect Dis 23:445-55. 2010
    ..After tuberculosis, leprosy (Mycobacterium leprae) and Buruli ulcer (M. ulcerans infection) are the second and third most common mycobacterial infections in humankind, respectively. Recent advances in both diseases are summarized...
  15. doi request reprint Short report: Clinical and molecular evidence for a case of Buruli ulcer (Mycobacterium ulcerans infection) in Kenya
    Douglas S Walsh
    Kenya Medical Research Institute Walter Reed Project, United States Army Medical Research Unit Kenya, Kisumu, Kenya
    Am J Trop Med Hyg 81:1110-3. 2009
    ..Buruli ulcer is named after Buruli County in Uganda (east Africa), where an epidemic occurred in the 1960s. Today, BU is most common in central and west Africa. We describe clinical and molecular evidence for a case of BU in Kenya...
  16. pmc Evaluation of in vitro cross-reactivity to avian H5N1 and pandemic H1N1 2009 influenza following prime boost regimens of seasonal influenza vaccination in healthy human subjects: a randomised trial
    Delia Bethell
    Department of Immunology and Medicine, Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand
    PLoS ONE 8:e59674. 2013
    ....
  17. pmc Inhibitory activity of ferroquine, versus chloroquine, against western Kenya Plasmodium falciparum field isolates determined by a SYBR Green I in vitro assay
    Fredrick L Eyase
    Department of Emerging Infectious Diseases Program, U S Army Medical Research Unit Kenya, Kenya Medical Research Institute Walter Reed Project, Kisumu, Kenya
    Am J Trop Med Hyg 85:984-8. 2011
    ..FQ is more potent than CQ against CQ-resistant P. falciparum field isolates and the W2 clone, and is less affected by Pfcrt 76T. These findings support the notion that FQ could be useful in treating persons with P. falciparum malaria...
  18. pmc Impact of RTS,S/AS02(A) and RTS,S/AS01(B) on genotypes of P. falciparum in adults participating in a malaria vaccine clinical trial
    John N Waitumbi
    Walter Reed Project, Kenya Medical Research Institute, Kisumu, Kenya
    PLoS ONE 4:e7849. 2009
    ..Parasite isolates from this study were used to determine the molecular impact of RTS,S/AS02(A) and RTS,S/AS01(B) on the multiplicity of infection (MOI) and the csp allelic characteristics of subsequent parasitemias...
  19. ncbi request reprint Heterologous prime-boost immunization in rhesus macaques by two, optimally spaced particle-mediated epidermal deliveries of Plasmodium falciparum circumsporozoite protein-encoding DNA, followed by intramuscular RTS,S/AS02A
    Douglas S Walsh
    Department of Immunology and Medicine, US Army Medical Component, Armed Forces Research Institute of Medical Sciences AFRIMS, Bangkok, Thailand
    Vaccine 24:4167-78. 2006
    ..In field studies, RTS,S/AS02A reduced malaria infection, clinical episodes, and disease severity. Heterologous prime-boost immunization regimens, optimally spaced, might improve the protective immunity of RTS,S/AS02A...
  20. ncbi request reprint Pre-clinical evaluation of the malaria vaccine candidate P. falciparum MSP1(42) formulated with novel adjuvants or with alum
    Sathit Pichyangkul
    Department of Immunology and Medicine, Armed Forces Research Institute of Medical Science AFRIMS, Bangkok, Thailand
    Vaccine 22:3831-40. 2004
    ..The notable difference in Th1/Th2 polarization induced by the AS02A and AS01B formulations warrants comparative clinical testing...
  21. ncbi request reprint Effective treatment of uncomplicated Plasmodium falciparum malaria with azithromycin-quinine combinations: a randomized, dose-ranging study
    R Scott Miller
    Department of Immunology and Medicine, Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand
    Am J Trop Med Hyg 74:401-6. 2006
    ..Three- and five-day azithromycin-quinine combination therapy appears safe, well tolerated, and effective in curing drug-resistant P. falciparum malaria. Further evaluation, especially in pediatric and obstetric populations, is warranted...
  22. ncbi request reprint Efficacy of monthly tafenoquine for prophylaxis of Plasmodium vivax and multidrug-resistant P. falciparum malaria
    Douglas S Walsh
    Department of Immunology and Medicine, United States Army Medical Component, Bangkok, Thailand
    J Infect Dis 190:1456-63. 2004
    ..falciparum malaria (95% CI, 60%-100%). Monthly tafenoquine was safe, well tolerated, and highly effective in preventing P. vivax and multidrug-resistant P. falciparum malaria in Thai soldiers during 6 months of prophylaxis...
  23. ncbi request reprint Safety and immunogenicity of rts,s+trap malaria vaccine, formulated in the as02a adjuvant system, in infant rhesus monkeys
    Douglas S Walsh
    Department of Immunology and Medicine, U S Army Medical Component, Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand
    Am J Trop Med Hyg 70:499-509. 2004
    ..RTS,S+TRAP/AS02A was safe and well tolerated. Vigorous antibody production and modest, selective cell-mediated immune responses suggest that RTS,S+TRAP/AS02A may be immunogenic in human infants...
  24. ncbi request reprint Robust in vitro replication of Plasmodium falciparum in glycosyl-phosphatidylinositol-anchored membrane glycoprotein-deficient red blood cells
    Kovit Pattanapanyasat
    Center of Excellence for Flow Cytometry, Office for Research and Development, Department of Medicine, Faculty of Medicine, Siriraj Hospital, Mahidol University, Bangkok, Thailand
    Am J Trop Med Hyg 69:360-5. 2003
    ..4 +/- 4.8%). Expression of DAF in 12 (63%) of 19 infected PNH samples was reduced. Thus, P. falciparum does not selectively avoid RBCs with fewer GPI proteins and parasite replication in PNH cells is at least as robust as in normal RBCs...