The point of maximum cell water volume excursion in case of presence of an impermeable soluteIgor I Katkov
Celltronix, 9263 Pebblestone Ln, San Diego, CA 92126, USA
Cryobiology 44:193-203. 2002
..Practical aspects of the relativistic permeability approach are also discussed...
- Cryoprotectant-free cryopreservation of human spermatozoa by vitrification and freezing in vapor: effect on motility, DNA integrity, and fertilization ability
Vladimir Isachenko
Department of Gynecological Endocrinology and Reproductive Medicine, University of Bonn, 53105 Bonn, Germany
Biol Reprod 71:1167-73. 2004
..Herein, we discuss the implications of this finding in the light of the physics of extra- and intracellular vitrification...
- Influence of exposure to vitrification solutions on 2-cell mouse embryos: II. Osmotic effects or chemical toxicity?
Igor I Katkov
UCSD Department of Pediatrics and The Burnham Institute for Medical Research, San Diego, California 92037, USA
Cryo Letters 28:409-27. 2007
..The osmotic damage vs. specific chemical toxicity of the vitrificants as the major cause of the elemental disturbance of intracellular potassium and sodium content are discussed...
On proper linearization, construction and analysis of the Boyle-van't Hoff plots and correct calculation of the osmotically inactive volumeIgor I Katkov
Stem Cell Center, Sanford Burnham Medical Reserch Institute, La Jolla, CA 92037, USA
Cryobiology 62:232-41. 2011
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- Challenge from the simple: some caveats in linearization of the Boyle-van't Hoff and Arrhenius plots
Igor I Katkov
Celltronix, San Diego, California, USA
Cryobiology 57:142-9. 2008
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- Prediction of the glass transition temperature of water solutions: comparison of different models
Igor I Katkov
UCSD Cancer Center, The Burnham Institute, 10901 North Torrey Pines, Bldg 7, r 7 105, La Jolla, CA, USA
Cryobiology 49:62-82. 2004
..Practical pitfalls related to determining water content, including experimental data on thermal gravimetry, are also discussed...
- Cryopreservation by slow cooling with DMSO diminished production of Oct-4 pluripotency marker in human embryonic stem cells
Igor I Katkov
UCSD Cancer Center, San Diego, CA 92122, USA; Stem Cell Center Core, Burnham Institute for Medical Research (BIMR, La Jolla, CA 92037, USA
Cryobiology 53:194-205. 2006
..It is concluded that new protocols and media are required for freezing hESC and safe storage at -80 degrees C as well as studies of the mechanisms of stress-related events associated with cell cryopreservation...
DMSO-Free Programmed Cryopreservation of Fully Dissociated and Adherent Human Induced Pluripotent Stem CellsIgor I Katkov
Celltronix, San Diego, CA 92196, USA
Stem Cells Int 2011:981606. 2011
..CP of cells directly in plates in ready-to-go after thawing format for HT/HC screening can be beneficial in many SC-related scientific and commercial applications such as drug discovery and toxicity tests...
- Changes in intracellular potassium and sodium content of 2-cell mouse embryos induced by exposition to vitrification concentrations of ethylene glycol
Alexander G Pogorelov
Institute of Theoretical and Experimental Biophysics, Moscow Region, Russia
Cryo Letters 27:87-98. 2006
..Possible mechanisms of changes in the intracellular elemental concentrations including the high intracellular sodium observed in intact embryos are discussed...
- Vitrification of human laser treated blastocysts within cut standard straws (CSS): novel aseptic packaging and reduced concentrations of cryoprotectants
Vladimir Isachenko
IVF Clinic AltraVita, Moscow, Russia
Cryobiology 54:305-9. 2007
..Our results indicate that the use of reduced concentrations of cryoprotectants and aseptic packaging of blastocysts did not have any statistically significant impact on the study outcomes...
- Influence of exposure to vitrification solutions on 2-cell mouse embryos: I. Intracellular potassium and sodium content
Alexander G Pogorelov
Institute of Theoretical and Experimental Biophysics, RAS, 142290 Puschino, Moscow Region, Russia
Cryo Letters 28:403-8. 2007
..Further exposure to 30%-EG for 3 min led to a two-fold decrease of both elements (60 +/- 3 mM and 66 +/- 2 mM for K and Na, respectively)...
- Clean technique for cryoprotectant-free vitrification of human spermatozoa
Vladimir Isachenko
Department of Endocrinology and Reproductive Medicine, University of Bonn, Sigmund Freud Strasse 25, 53127 Bonn, Germany
Reprod Biomed Online 10:350-4. 2005
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- Vitrification of mammalian spermatozoa in the absence of cryoprotectants: from past practical difficulties to present success
Eugenia Isachenko
Department of Obstetrics and Gynecology, University of Cologne, Kerpener Strasse 34, 50931 Cologne, Germany
Reprod Biomed Online 6:191-200. 2003
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