Roger L Kaspar

Summary

Publications

  1. pmc Visualization of plasmid delivery to keratinocytes in mouse and human epidermis
    Emilio Gonzalez-Gonzalez
    Molecular Imaging Program at Stanford MIPS, Stanford University School of Medicine, Stanford, CA 94305, USA
    Sci Rep 1:158. 2011
  2. doi request reprint Use of self-delivery siRNAs to inhibit gene expression in an organotypic pachyonychia congenita model
    Robyn P Hickerson
    TransDerm Inc, Santa Cruz, California, USA
    J Invest Dermatol 131:1037-44. 2011
  3. pmc Stability study of unmodified siRNA and relevance to clinical use
    Robyn P Hickerson
    TransDerm Inc, Santa Cruz, California, USA
    Oligonucleotides 18:345-54. 2008
  4. ncbi request reprint Delivery and inhibition of reporter genes by small interfering RNAs in a mouse skin model
    Qian Wang
    Molecular Imaging Program at Stanford, and Department of Radiology, Stanford University School of Medicine, Stanford, California, USA
    J Invest Dermatol 127:2577-84. 2007
  5. ncbi request reprint Single-nucleotide-specific siRNA targeting in a dominant-negative skin model
    Robyn P Hickerson
    TransDerm Inc, Santa Cruz, California, USA
    J Invest Dermatol 128:594-605. 2008
  6. pmc Silencing of reporter gene expression in skin using siRNAs and expression of plasmid DNA delivered by a soluble protrusion array device (PAD)
    Emilio Gonzalez-Gonzalez
    Department of Pediatrics, Stanford University School of Medicine, Stanford, California, USA
    Mol Ther 18:1667-74. 2010
  7. doi request reprint Development of quantitative molecular clinical end points for siRNA clinical trials
    Robyn P Hickerson
    TransDerm, Santa Cruz, California 95060, USA
    J Invest Dermatol 131:1029-36. 2011
  8. doi request reprint Biodegradable nanoparticles with sustained release of functional siRNA in skin
    Gunilla B Jacobson
    Department of Chemistry, Stanford University, Stanford, California 94305, USA
    J Pharm Sci 99:4261-6. 2010
  9. ncbi request reprint shRNAs targeting hepatitis C: effects of sequence and structural features, and comparision with siRNA
    Alexander V Vlassov
    SomaGenics, Inc, Santa Cruz, CA 95060, USA
    Oligonucleotides 17:223-36. 2007
  10. pmc Inhibition of CD44 gene expression in human skin models, using self-delivery short interfering RNA administered by dissolvable microneedle arrays
    Maria Fernanda Lara
    TransDerm, Santa Cruz, CA 95060, USA
    Hum Gene Ther 23:816-23. 2012

Collaborators

Detail Information

Publications20

  1. pmc Visualization of plasmid delivery to keratinocytes in mouse and human epidermis
    Emilio Gonzalez-Gonzalez
    Molecular Imaging Program at Stanford MIPS, Stanford University School of Medicine, Stanford, CA 94305, USA
    Sci Rep 1:158. 2011
    ..These studies revealed that non-invasive intravital imaging can be used as a guide for developing gene delivery tools, establishing a benchmark for comparative testing of nucleic acid skin delivery technologies...
  2. doi request reprint Use of self-delivery siRNAs to inhibit gene expression in an organotypic pachyonychia congenita model
    Robyn P Hickerson
    TransDerm Inc, Santa Cruz, California, USA
    J Invest Dermatol 131:1037-44. 2011
    ..These results indicate that chemical modification of siRNA may overcome certain limitations to transdermal delivery (specifically keratinocyte uptake) and may have clinical utility for inhibition of gene expression in the skin...
  3. pmc Stability study of unmodified siRNA and relevance to clinical use
    Robyn P Hickerson
    TransDerm Inc, Santa Cruz, California, USA
    Oligonucleotides 18:345-54. 2008
    ..Taken together, these data indicate that unmodified siRNAs are viable therapeutic candidates...
  4. ncbi request reprint Delivery and inhibition of reporter genes by small interfering RNAs in a mouse skin model
    Qian Wang
    Molecular Imaging Program at Stanford, and Department of Radiology, Stanford University School of Medicine, Stanford, California, USA
    J Invest Dermatol 127:2577-84. 2007
    ..These results indicate that small interfering RNA, delivered locally as RNA directly or expressed from viral or non-viral vectors, may be effective agents for treating skin disorders...
  5. ncbi request reprint Single-nucleotide-specific siRNA targeting in a dominant-negative skin model
    Robyn P Hickerson
    TransDerm Inc, Santa Cruz, California, USA
    J Invest Dermatol 128:594-605. 2008
    ..These results suggest that efficient delivery of these "designer siRNAs" may allow effective treatment of numerous genetic disorders including PC...
  6. pmc Silencing of reporter gene expression in skin using siRNAs and expression of plasmid DNA delivered by a soluble protrusion array device (PAD)
    Emilio Gonzalez-Gonzalez
    Department of Pediatrics, Stanford University School of Medicine, Stanford, California, USA
    Mol Ther 18:1667-74. 2010
    ..These results support the use of PADs for delivery of functional nucleic acids to cells in the skin with an efficiency that may support clinical translation...
  7. doi request reprint Development of quantitative molecular clinical end points for siRNA clinical trials
    Robyn P Hickerson
    TransDerm, Santa Cruz, California 95060, USA
    J Invest Dermatol 131:1029-36. 2011
    ....
  8. doi request reprint Biodegradable nanoparticles with sustained release of functional siRNA in skin
    Gunilla B Jacobson
    Department of Chemistry, Stanford University, Stanford, California 94305, USA
    J Pharm Sci 99:4261-6. 2010
    ..In vivo gene silencing experiments were also performed, showing reduction of GFP signal in the epidermis of a reporter transgenic mouse model, which demonstrates that the siRNA retained activity following release from the polymer NPs...
  9. ncbi request reprint shRNAs targeting hepatitis C: effects of sequence and structural features, and comparision with siRNA
    Alexander V Vlassov
    SomaGenics, Inc, Santa Cruz, CA 95060, USA
    Oligonucleotides 17:223-36. 2007
    ..The results indicate that shRNAs, which can be prepared by either transcription or chemical synthesis, may be effective agents for the control of HCV...
  10. pmc Inhibition of CD44 gene expression in human skin models, using self-delivery short interfering RNA administered by dissolvable microneedle arrays
    Maria Fernanda Lara
    TransDerm, Santa Cruz, CA 95060, USA
    Hum Gene Ther 23:816-23. 2012
    ..Taken together, these results demonstrate that sd-siRNA, delivered by microneedle arrays, can reduce expression of a targeted endogenous gene in a human skin xenograft model...
  11. pmc Assessing delivery and quantifying efficacy of small interfering ribonucleic acid therapeutics in the skin using a dual-axis confocal microscope
    Hyejun Ra
    Stanford University, Department of Electrical Engineering, Ginzton Laboratory, Stanford, California 94305, USA
    J Biomed Opt 15:036027. 2010
    ..Visualization of transdermal delivery of nucleic acids will play an important role in the development of innovative strategies for treating skin pathologies...
  12. pmc In vivo imaging of human and mouse skin with a handheld dual-axis confocal fluorescence microscope
    Hyejun Ra
    James H Clark Center for Biomedical Engineering and Sciences, Department of Pediatrics, Stanford University, Stanford, California 94305, USA
    J Invest Dermatol 131:1061-6. 2011
    ..These results suggest that in vivo confocal microscopy may provide an informative clinical end point to evaluate the efficacy of experimental molecular therapeutics...
  13. pmc Intravital fluorescence imaging of small interfering RNA-mediated gene repression in a dual reporter melanoma xenograft model
    Robyn P Hickerson
    TransDerm Inc, Santa Cruz, California, USA
    Nucleic Acid Ther 22:438-43. 2012
    ..No effect was observed with nonspecific control siRNA treatment. This model provides a platform on which siRNA delivery technologies can be screened and optimized in vivo...
  14. ncbi request reprint Inhibition of hepatitis C IRES-mediated gene expression by small hairpin RNAs in human hepatocytes and mice
    Heini Ilves
    SomaGenics, Inc, Santa Cruz, CA 95060, USA
    Ann N Y Acad Sci 1082:52-5. 2006
    ..The results indicate that shRNAs, delivered as naked RNA or expressed from vectors, may be effective agents for the control of HCV and related viruses...
  15. pmc Hairpin ribozyme-antisense RNA constructs can act as molecular Lassos
    Anne Dallas
    SomaGenics, Inc, Santa Cruz, CA 95060, USA
    Nucleic Acids Res 36:6752-66. 2008
    ..We also show in cell culture experiments that Lassos directed against Fas pre-mRNA were able to induce a change in alternative splicing patterns...
  16. doi request reprint Rapamycin selectively inhibits expression of an inducible keratin (K6a) in human keratinocytes and improves symptoms in pachyonychia congenita patients
    Robyn P Hickerson
    TransDerm, Inc, Santa Cruz, CA, USA
    J Dermatol Sci 56:82-8. 2009
    ..Published sequence data suggest the 5' untranslated regions of K6a and K6b mRNAs contain 5' TOP motifs and therefore may be sensitive to rapamycin treatment...
  17. ncbi request reprint Small hairpin RNAs efficiently inhibit hepatitis C IRES-mediated gene expression in human tissue culture cells and a mouse model
    Qian Wang
    Molecular Imaging Program at Stanford, Department of Radiology, and Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA 94305, USA
    Mol Ther 12:562-8. 2005
    ..These results indicate that shRNAs, delivered as RNA or expressed from viral or nonviral vectors, may be effective agents for the control of HCV and related viruses...
  18. ncbi request reprint SiRNA-mediated selective inhibition of mutant keratin mRNAs responsible for the skin disorder pachyonychia congenita
    Robyn P Hickerson
    TransDerm, Santa Cruz, California 95060, USA
    Ann N Y Acad Sci 1082:56-61. 2006
    ..These studies suggest that siRNAs can discriminate single nucleotide mutations and further suggest that "designer siRNAs" may allow effective treatment of a host of genetic disorders including PC...
  19. pmc Designed guanidinium-rich amphipathic oligocarbonate molecular transporters complex, deliver and release siRNA in cells
    Erika I Geihe
    Department of Chemistry, Stanford University, Stanford, CA 94305, USA
    Proc Natl Acad Sci U S A 109:13171-6. 2012
    ..The speed and versatility of this approach and the biodegradability of the designed agents make this an attractive strategy for biological tool development, imaging, diagnostics, and therapeutic applications...
  20. doi request reprint Nanoparticle formation of organic compounds with retained biological activity
    Gunilla B Jacobson
    Department of Chemistry, Stanford University, 333 Campus Drive, Stanford, California 94305 5080, USA
    J Pharm Sci 99:2750-5. 2010
    ..In both cases we achieve retention of bioactivity as well as a narrow particle size distribution in which the particles are free of impurities...