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Incorporation of non-natural nucleotides into template-switching oligonucleotides reduces background and improves cDNA synthesis from very small RNA samplesJeremy Kapteyn
School of Plant Sciences and BIO5 Institute, The University of Arizona, 1657 E Helen Street, Tucson, AZ 85721, USA
BMC Genomics 11:413. 2010..However, when applied to very small RNA samples, such as those obtained from tens or hundreds of cells, this approach leads to high background and low cDNA yield due to concatamerization of the TS oligo...
