Stefan H I Kappe

Summary

Publications

  1. pmc Plasmodium falciparum PF10_0164 (ETRAMP10.3) is an essential parasitophorous vacuole and exported protein in blood stages
    Drew C MacKellar
    Seattle Biomedical Research Institute, 307 Westlake Avenue North, Suite 500, Seattle, WA 98109 5219, USA
    Eukaryot Cell 9:784-94. 2010
  2. pmc A rapid and scalable density gradient purification method for Plasmodium sporozoites
    Mark Kennedy
    Center for Mosquito Production and Malaria Infection Research, Seattle Biomedical Research Institute, 307 Westlake Avenue North, Suite 500, Seattle, WA 98109, USA
    Malar J 11:421. 2012
  3. doi request reprint That was then but this is now: malaria research in the time of an eradication agenda
    Stefan H I Kappe
    Seattle Biomedical Research Institute, Seattle, WA 98109, USA
    Science 328:862-6. 2010
  4. pmc Distinct malaria parasite sporozoites reveal transcriptional changes that cause differential tissue infection competence in the mosquito vector and mammalian host
    Sebastian A Mikolajczak
    Seattle Biomedical Research Institute, Seattle, WA 98109 5219, USA
    Mol Cell Biol 28:6196-207. 2008
  5. pmc Complete Plasmodium falciparum liver-stage development in liver-chimeric mice
    Ashley M Vaughan
    Seattle Biomedical Research Institute, Seattle, Washington 98109, USA
    J Clin Invest 122:3618-28. 2012
  6. pmc Targeted deletion of SAP1 abolishes the expression of infectivity factors necessary for successful malaria parasite liver infection
    Ahmed S I Aly
    Seattle Biomedical Research Institute, Seattle, WA 98109, USA
    Mol Microbiol 69:152-63. 2008
  7. pmc Type II fatty acid synthesis is essential only for malaria parasite late liver stage development
    Ashley M Vaughan
    Seattle Biomedical Research Institute, Seattle, WA 98109, USA
    Cell Microbiol 11:506-20. 2009
  8. pmc Total and putative surface proteomics of malaria parasite salivary gland sporozoites
    Scott E Lindner
    Malaria Program, Seattle Biomedical Research Institute, 307 Westlake Avenue North, Suite 500, Seattle, Washington 98109, USA
    Mol Cell Proteomics 12:1127-43. 2013
  9. doi request reprint A transgenic Plasmodium falciparum NF54 strain that expresses GFP-luciferase throughout the parasite life cycle
    Ashley M Vaughan
    Seattle Biomedical Research Institute, Seattle, WA, USA
    Mol Biochem Parasitol 186:143-7. 2012
  10. pmc Model for in vivo assessment of humoral protection against malaria sporozoite challenge by passive transfer of monoclonal antibodies and immune serum
    Brandon K Sack
    Seattle Biomedical Research Institute, Seattle, Washington, USA
    Infect Immun 82:808-17. 2014

Collaborators

Detail Information

Publications56

  1. pmc Plasmodium falciparum PF10_0164 (ETRAMP10.3) is an essential parasitophorous vacuole and exported protein in blood stages
    Drew C MacKellar
    Seattle Biomedical Research Institute, 307 Westlake Avenue North, Suite 500, Seattle, WA 98109 5219, USA
    Eukaryot Cell 9:784-94. 2010
    ..We conclude that PF10_0164 is a parasitophorous vacuole protein that is essential in asexual blood stages and that does not complement P. yoelii UIS4, and it is thus likely not a functional ortholog of UIS4...
  2. pmc A rapid and scalable density gradient purification method for Plasmodium sporozoites
    Mark Kennedy
    Center for Mosquito Production and Malaria Infection Research, Seattle Biomedical Research Institute, 307 Westlake Avenue North, Suite 500, Seattle, WA 98109, USA
    Malar J 11:421. 2012
    ..However, sporozoites can only be produced in and isolated from mosquitoes, and their isolation results in large amounts of accompanying mosquito debris and contaminating microbes...
  3. doi request reprint That was then but this is now: malaria research in the time of an eradication agenda
    Stefan H I Kappe
    Seattle Biomedical Research Institute, Seattle, WA 98109, USA
    Science 328:862-6. 2010
    ..Here, we highlight some of the research into malaria parasite biology that has the potential to provide new intervention targets for antimalarial drugs and vaccines...
  4. pmc Distinct malaria parasite sporozoites reveal transcriptional changes that cause differential tissue infection competence in the mosquito vector and mammalian host
    Sebastian A Mikolajczak
    Seattle Biomedical Research Institute, Seattle, WA 98109 5219, USA
    Mol Cell Biol 28:6196-207. 2008
    ..Genes identified herein might represent targets for vector-based transmission blocking strategies (UOS genes), as well as strategies that prevent mammalian host infection (UIS genes)...
  5. pmc Complete Plasmodium falciparum liver-stage development in liver-chimeric mice
    Ashley M Vaughan
    Seattle Biomedical Research Institute, Seattle, Washington 98109, USA
    J Clin Invest 122:3618-28. 2012
    ..Thus, these mice constitute reliable models to study human LS directly in vivo and demonstrate utility for studies of LS-to-blood-stage transition of a human malaria parasite...
  6. pmc Targeted deletion of SAP1 abolishes the expression of infectivity factors necessary for successful malaria parasite liver infection
    Ahmed S I Aly
    Seattle Biomedical Research Institute, Seattle, WA 98109, USA
    Mol Microbiol 69:152-63. 2008
    ..These findings demonstrate that SAP1 is essential for liver infection possibly by functioning as a selective regulator controlling the expression of infectivity-associated parasite effector genes...
  7. pmc Type II fatty acid synthesis is essential only for malaria parasite late liver stage development
    Ashley M Vaughan
    Seattle Biomedical Research Institute, Seattle, WA 98109, USA
    Cell Microbiol 11:506-20. 2009
    ..Malaria parasites therefore depend on the intrinsic FAS II pathway only at one specific life cycle transition point, from liver to blood...
  8. pmc Total and putative surface proteomics of malaria parasite salivary gland sporozoites
    Scott E Lindner
    Malaria Program, Seattle Biomedical Research Institute, 307 Westlake Avenue North, Suite 500, Seattle, Washington 98109, USA
    Mol Cell Proteomics 12:1127-43. 2013
    ....
  9. doi request reprint A transgenic Plasmodium falciparum NF54 strain that expresses GFP-luciferase throughout the parasite life cycle
    Ashley M Vaughan
    Seattle Biomedical Research Institute, Seattle, WA, USA
    Mol Biochem Parasitol 186:143-7. 2012
    ..This parasite reporter strain will accelerate testing of interventions against pre-erythrocytic life cycle stages...
  10. pmc Model for in vivo assessment of humoral protection against malaria sporozoite challenge by passive transfer of monoclonal antibodies and immune serum
    Brandon K Sack
    Seattle Biomedical Research Institute, Seattle, Washington, USA
    Infect Immun 82:808-17. 2014
    ..falciparum challenge model. Together, these models constitute unique and sensitive in vivo methods to assess serum-transferable protection against Plasmodium sporozoite challenge. ..
  11. doi request reprint An efficient strategy for gene targeting and phenotypic assessment in the Plasmodium yoelii rodent malaria model
    Sebastian A Mikolajczak
    Seattle Biomedical Research Institute, Seattle, WA 98109, USA
    Mol Biochem Parasitol 158:213-6. 2008
    ..This allows for a more rapid assessment of parasite growth in all of its developmental stages. In addition, the introduction of the fluorescent marker via the replacement strategy confers the stable integration of the marker...
  12. ncbi request reprint Protracted sterile protection with Plasmodium yoelii pre-erythrocytic genetically attenuated parasite malaria vaccines is independent of significant liver-stage persistence and is mediated by CD8+ T cells
    Alice S Tarun
    Seattle Biomedical Research Institute, Seattle, WA 98109, USA
    J Infect Dis 196:608-16. 2007
    ..These findings have important implications for the development of a P. falciparum GAP malaria vaccine...
  13. pmc Plasmodium yoelii sporozoites with simultaneous deletion of P52 and P36 are completely attenuated and confer sterile immunity against infection
    Mehdi Labaied
    Seattle Biomedical Research Institute, 307 Westlake Avenue North, Suite 500, Seattle, WA 98109 5219, USA
    Infect Immun 75:3758-68. 2007
    ..The study will critically guide the design of Plasmodium falciparum live attenuated malaria vaccines...
  14. pmc Disruption of the Plasmodium falciparum liver-stage antigen-1 locus causes a differentiation defect in late liver-stage parasites
    Sebastian A Mikolajczak
    Seattle Biomedical Research Institute, Seattle, WA 98109, USA
    Cell Microbiol 13:1250-60. 2011
    ..LSA-1 is the first P. falciparum protein identified to be required for this transitional stage of the parasite life cycle...
  15. doi request reprint SAP1 is a critical post-transcriptional regulator of infectivity in malaria parasite sporozoite stages
    Ahmed S I Aly
    Seattle Biomedical Research Institute, Seattle, WA 98109, USA
    Mol Microbiol 79:929-39. 2011
    ..SAP1 is therefore an appealing candidate locus for attenuation of Plasmodium falciparum...
  16. pmc Plasmodium yoelii macrophage migration inhibitory factor is necessary for efficient liver-stage development
    Jessica L Miller
    Seattle Biomedical Research Institute, Seattle, Washington, USA
    Infect Immun 80:1399-407. 2012
    ..Combined, the data indicate that Plasmodium MIF is important for liver-stage development of P. yoelii, during which it is likely to play an intrinsic role in parasite development rather than modulating host immune responses to infection...
  17. pmc Preerythrocytic, live-attenuated Plasmodium falciparum vaccine candidates by design
    Kelley M VanBuskirk
    Seattle Biomedical Research Institute, Seattle, WA 98109, USA
    Proc Natl Acad Sci U S A 106:13004-9. 2009
    ..GAPs might provide a safe and reproducible platform to develop an efficacious whole-cell malaria vaccine that prevents infection at the preerythrocytic stage...
  18. ncbi request reprint Vaccination using radiation- or genetically attenuated live sporozoites
    Ashley M Vaughan
    Seattle Biomedical Research Institute, University of Washington, Seattle, WA, USA
    Methods Mol Biol 923:549-66. 2013
    ....
  19. pmc A systematic analysis of the early transcribed membrane protein family throughout the life cycle of Plasmodium yoelii
    Drew C MacKellar
    Molecular and Cellular Biology Program, University of Washington, Seattle, WA, USA
    Cell Microbiol 13:1755-67. 2011
    ..Systematic characterization of the members of the ETRAMP family reveals the diversity in importance of each family member at the interface between host and parasite throughout the developmental cycle of the malaria parasite...
  20. pmc Susceptibility to Plasmodium yoelii preerythrocytic infection in BALB/c substrains is determined at the point of hepatocyte invasion
    Alexis Kaushansky
    Seattle Biomedical Research Institute, Seattle, Washington, USA
    Infect Immun 83:39-47. 2015
    ..These data suggest that a yet-unknown receptor for sporozoite infection, present at elevated levels on BALB/cByJ hepatocytes and also polyploid hepatocytes, might facilitate Plasmodium liver infection. ..
  21. pmc Malaria parasite pre-erythrocytic infection: preparation meets opportunity
    Scott E Lindner
    Seattle Biomedical Research Institute, Seattle, WA 98109, USA
    Cell Microbiol 14:316-24. 2012
    ..Here we review relevant findings on how sporozoites prepare for infection of the liver and factors crucial to liver stage development as well as key host/parasite interactions...
  22. pmc Plasmodium yoelii inhibitor of cysteine proteases is exported to exomembrane structures and interacts with yoelipain-2 during asexual blood-stage development
    Ying Pei
    Seattle Biomedical Research Institute, 307 Westlake Avenue North, Suite 500, Seattle, WA 98109, USA
    Cell Microbiol 15:1508-26. 2013
    ..These data show that ICP may be important in regulating proteolytic processes during blood-stage development, and is likely playing a role in liver stage-hepatocyte interactions at the time of exoerythrocytic merozoite release. ..
  23. pmc Suppression of host p53 is critical for Plasmodium liver-stage infection
    Alexis Kaushansky
    Malaria Program, Seattle Biomedical Research Institute, Seattle, WA 98109, USA
    Cell Rep 3:630-7. 2013
    ..We conclude that perturbation of the hepatocyte p53 pathway critically impacts parasite survival. Thus, host pathways might constitute potential targets for host-based antimalarial prophylaxis...
  24. pmc Perturbations of Plasmodium Puf2 expression and RNA-seq of Puf2-deficient sporozoites reveal a critical role in maintaining RNA homeostasis and parasite transmissibility
    Scott E Lindner
    Seattle Biomedical Research Institute, Seattle, WA 98109, USA
    Cell Microbiol 15:1266-83. 2013
    ..These findings uncover requirements for maintaining a window of opportunity for the malaria parasite to accommodate the unpredictable moment of transmission from mosquito to mammalian host...
  25. doi request reprint Plasmodium pyruvate dehydrogenase activity is only essential for the parasite's progression from liver infection to blood infection
    Ying Pei
    Seattle Biomedical Research Institute, 307 Westlake Avenue North, Suite 500, Seattle, WA 98109, USA
    Mol Microbiol 75:957-71. 2010
    ..This phenotype is similar to that observed for deletions of genes involved in FAS II elongation. The data strongly support the hypothesis that the sole role of PDH is to provide acetyl-CoA for FAS II...
  26. pmc A combined transcriptome and proteome survey of malaria parasite liver stages
    Alice S Tarun
    Seattle Biomedical Research Institute, Seattle, WA 98109, USA
    Proc Natl Acad Sci U S A 105:305-10. 2008
    ....
  27. doi request reprint Subpatent infection with nucleoside transporter 1-deficient Plasmodium blood stage parasites confers sterile protection against lethal malaria in mice
    Ahmed S I Aly
    Seattle Biomedical Research Institute, Seattle, WA 98109, USA
    Cell Microbiol 12:930-8. 2010
    ..The study demonstrates that genetic manipulation provides a platform for the designed, complete attenuation of malaria parasite blood stages and suggests testing the safety and efficacy of P. falciparum NT1 knockout strains in humans...
  28. doi request reprint Redefining the role of de novo fatty acid synthesis in Plasmodium parasites
    Alice S Tarun
    Seattle Biomedical Research Institute, Seattle, WA 98109, USA Infectious Diseases and Microbiology, University of Pittsburgh PA 15261, USA
    Trends Parasitol 25:545-50. 2009
    ..We discuss the role of fatty acid metabolism in Plasmodium and why we believe that de novo fatty acid synthesis is only required for parasite late liver-stage development...
  29. pmc Quantitative bioluminescent imaging of pre-erythrocytic malaria parasite infection using luciferase-expressing Plasmodium yoelii
    Jessica L Miller
    Seattle Biomedical Research Institute, Seattle, Washington, USA
    PLoS ONE 8:e60820. 2013
    ..Thus, this rapid, simple and noninvasive method for monitoring P. yoelii infection in the liver provides an efficient system to screen and evaluate the effects of anti-malarial interventions in vivo and in real-time...
  30. pmc Malaria parasite pre-erythrocytic stage infection: gliding and hiding
    Ashley M Vaughan
    Seattle Biomedical Research Institute, Seattle, WA 98109, USA
    Cell Host Microbe 4:209-18. 2008
    ..We discuss the factors, both parasite and host, involved in the interactions that occur during this "silent" phase of infection...
  31. pmc Genetically engineered, attenuated whole-cell vaccine approaches for malaria
    Ashley M Vaughan
    Seattle Biomedical Research Institute, Seattle, WA, USA
    Hum Vaccin 6:107-13. 2010
    ....
  32. pmc A dispensable Plasmodium locus for stable transgene expression
    Vanessa Y Jacobs-Lorena
    Seattle Biomedical Research Institute, Seattle, WA 98109 5219, USA
    Mol Biochem Parasitol 171:40-4. 2010
    ..yoelii wildtype parasites. Further, we show that a fluorescent transgene can be stably expressed from this site. This demonstrates that the S1 locus can be utilized for stable expression of heterologous genes in rodent malaria parasites...
  33. pmc Host-based Prophylaxis Successfully Targets Liver Stage Malaria Parasites
    Alyse N Douglass
    Seattle Biomedical Research Institute, Seattle, Washington, United States
    Mol Ther 23:857-65. 2015
    ..Our data demonstrate that host-based prophylaxis could be developed into an effective intervention strategy that eliminates LS parasites before the onset of clinical disease and thus opens a new avenue to prevent malaria. ..
  34. pmc A next-generation genetically attenuated Plasmodium falciparum parasite created by triple gene deletion
    Sebastian A Mikolajczak
    Seattle Biomedical Research Institute Seattle Biomed, Seattle, Washington, USA
    Mol Ther 22:1707-15. 2014
    ..Thus, clinical testing of Pf p52(-)/p36(-)/sap1(-) GAP assessing safety, immunogenicity, and efficacy against sporozoite challenge is warranted. ..
  35. doi request reprint Plasmodium vivax Liver Stage Development and Hypnozoite Persistence in Human Liver-Chimeric Mice
    Sebastian A Mikolajczak
    Seattle Biomedical Research Institute, 307 Westlake Avenue North, Suite 500, Seattle, WA 98109, USA Electronic address
    Cell Host Microbe 17:526-35. 2015
    ..Thus, P. vivax-infected FRG KO huHep mice are a model to investigate liver stage development and dormancy and may facilitate the discovery of drugs targeting relapsing malaria. ..
  36. doi request reprint Malaria vaccine development: persistent challenges
    Ashley M Vaughan
    Seattle Biomedical Research Institute, 307 Westlake Avenue North, Seattle, WA 98109, USA
    Curr Opin Immunol 24:324-31. 2012
    ..Whole parasite approaches to vaccine design through attenuation as well as subunit vaccine development continue to move forward to clinical trials and are showing promising results...
  37. pmc Development of a quantitative flow cytometry-based assay to assess infection by Plasmodium falciparum sporozoites
    Alexis Kaushansky
    Seattle Biomedical Research Institute, Seattle, WA, United States
    Mol Biochem Parasitol 183:100-3. 2012
    ..This methodology will aid in assessing functional antibody responses to vaccination and novel drugs that prevent mosquito-to-man transmission of malaria...
  38. ncbi request reprint L-FABP is a critical host factor for successful malaria liver stage development
    Sebastian A Mikolajczak
    Seattle Biomedical Research Institute, 307 Westlake Avenue North, Suite 500, Seattle, WA 98109 5219, USA
    Int J Parasitol 37:483-9. 2007
    ..This is the first identified direct liver stage-host cell protein interaction, providing a possible explanation for the importance of UIS3 in liver infection...
  39. pmc Advances and challenges in malaria vaccine development
    Ruobing Wang
    Seattle Biomedical Research Institute and Department of Global Health, University of Washington, Seattle, Washington, USA
    Expert Rev Mol Med 11:e39. 2009
    ..Elimination of malaria will probably ultimately depend on the development of highly effective vaccines...
  40. ncbi request reprint Depletion of the Plasmodium berghei thrombospondin-related sporozoite protein reveals a role in host cell entry by sporozoites
    Mehdi Labaied
    Seattle Biomedical Research Institute, Seattle, WA 98109, USA
    Mol Biochem Parasitol 153:158-66. 2007
    ..Thus, TRSP is an additional TSR-containing malaria parasite protein that is mainly involved in initial infection of the mammalian host...
  41. pmc Immunization of mice with live-attenuated late liver stage-arresting Plasmodium yoelii parasites generates protective antibody responses to preerythrocytic stages of malaria
    Gladys J Keitany
    Seattle Biomedical Research Institute, Seattle, Washington, USA
    Infect Immun 82:5143-53. 2014
    ..Furthermore, they highlight the importance of considering both the route of challenge and the genetic background of the mouse strains used when interpreting vaccine efficacy studies in animal models of malaria infection. ..
  42. ncbi request reprint Preerythrocytic malaria vaccine development
    Sebastian A Mikolajczak
    Seattle Biomedical Research Institute, Seattle, Washington 98109 5219, USA
    Curr Opin Infect Dis 20:461-6. 2007
    ..This review examines the potential of current preerythrocytic stage malaria vaccine approaches to reduce the global burden of malaria...
  43. pmc Malaria parasite development in the mosquito and infection of the mammalian host
    Ahmed S I Aly
    Seattle Biomedical Research Institute, Seattle, Washington 98109, USA
    Annu Rev Microbiol 63:195-221. 2009
    ..Here, we discuss what is known about the molecular and cellular basis of the developmental progression of parasites and their interactions with host tissues in the mosquito and during the early phase of mammalian infection...
  44. pmc Cyclic GMP balance is critical for malaria parasite transmission from the mosquito to the mammalian host
    Viswanathan Lakshmanan
    Seattle Biomedical Research Institute Seattle Biomed, Seattle, Washington, USA
    MBio 6:e02330. 2015
    ..Our data reveal a crucial role for PDEγ in maintaining the cyclic nucleotide balance in the malaria parasite sporozoite stage, which in turn is essential for parasite transmission from mosquito to mammal...
  45. pmc SSP3 is a novel Plasmodium yoelii sporozoite surface protein with a role in gliding motility
    Anke Harupa
    Seattle Biomedical Research Institute, Seattle, Washington, USA Institute of Biology, Freie Universitaet Berlin, Berlin, Germany
    Infect Immun 82:4643-53. 2014
    ..Together, these data reveal a previously unappreciated complexity of the Plasmodium sporozoite surface proteome and the roles of surface proteins in distinct biological activities of sporozoites. ..
  46. doi request reprint Interferon-mediated innate immune responses against malaria parasite liver stages
    Jessica L Miller
    Seattle Biomedical Research Institute, 307 Westlake Avenue North, Suite 500, Seattle, WA 98109, USA
    Cell Rep 7:436-47. 2014
    ..Our findings demonstrate innate immune sensing of malaria parasite liver-stage infection and that the ensuing innate responses can eliminate the parasite...
  47. pmc Enzymes involved in plastid-targeted phosphatidic acid synthesis are essential for Plasmodium yoelii liver-stage development
    Scott E Lindner
    Seattle Biomedical Research Institute, Seattle, WA, 98109, USA
    Mol Microbiol 91:679-93. 2014
    ..Our results suggest that P. yoelii has an incomplete apicoplast-targeted phosphatidic acid synthesis pathway that is essential for liver-stage maturation...
  48. pmc Short-lived effector CD8 T cells induced by genetically attenuated malaria parasite vaccination express CD11c
    Laura A Cooney
    Seattle Biomedical Research Institute, Seattle, Washington, USA
    Infect Immun 81:4171-81. 2013
    ..Together, these results suggest that CD11c marks a subset of highly inflammatory, short-lived, antigen-specific effector cells, which may play an important role in eliminating infected hepatocytes. ..
  49. pmc Real-time quantitative reverse transcription PCR for monitoring of blood-stage Plasmodium falciparum infections in malaria human challenge trials
    Sean C Murphy
    Department of Laboratory Medicine, Division of Allergy and Infectious Diseases, University of Washington Medical Center, Seattle, 98195 7110, USA
    Am J Trop Med Hyg 86:383-94. 2012
    ..7 days earlier on average than thick blood smears. This validated, internally controlled qRT-PCR method also uses a small (50 μL) sample volume requiring minimal pre-analytical handling, making it useful for clinical trials...
  50. doi request reprint Model for In Vivo Assessment of Humoral Protection against Malaria Sporozoite Challenge by Passive Transfer of Monoclonal Antibodies and Immune Serum
    Brandon K Sack
    Seattle Biomedical Research Institute, Seattle, Washington, USA
    Infect Immun 82:808-17. 2014
    ..falciparum challenge model. Together, these models constitute unique and sensitive in vivo methods to assess serum-transferable protection against Plasmodium sporozoite challenge. ..
  51. pmc Development of humanized mouse models to study human malaria parasite infection
    Ashley M Vaughan
    Seattle Biomedical Research Institute, WA 98109, USA
    Future Microbiol 7:657-65. 2012
    ..falciparum infection that will accelerate fundamental research into human parasite biology and could accelerate drug and vaccine design in the future...
  52. pmc Assessment and improvement of the Plasmodium yoelii yoelii genome annotation through comparative analysis
    Ashley Vaughan
    Seattle Biomedical Research Institute, Seattle, WA 98109, USA
    Bioinformatics 24:i383-9. 2008
    ..Unfortunately, only preliminary gene models were annotated on the partially sequenced genome, mostly by in silico gene prediction, and there has been no major improvement of the annotation since 2002...
  53. pmc Plasmodium falciparum genetic crosses in a humanized mouse model
    Ashley M Vaughan
    Center for Infectious Disease Research formerly Seattle Biomedical Research Institute, Seattle, Washington, USA
    Nat Methods 12:631-3. 2015
    ..Here we demonstrate that human-liver chimeric mice support recovery of recombinant progeny for the identification of genetic determinants of parasite traits and adaptations. ..
  54. pmc Of men in mice: the success and promise of humanized mouse models for human malaria parasite infections
    Alexis Kaushansky
    Seattle Biomedical Research Institute, Seattle, WA, 98109, USA
    Cell Microbiol 16:602-11. 2014
    ....
  55. pmc Susceptibility to Plasmodium liver stage infection is altered by hepatocyte polyploidy
    Laura S Austin
    Department of Global Health, University of Washington, Seattle, WA, USA Seattle Biomedical Research Institute, Seattle, WA, USA
    Cell Microbiol 16:784-95. 2014
    ..This parasite preference for host cells of high ploidy cannot be explained by differences in hepatocyte size or DNA replication. We conclude that Plasmodium preferentially infects and develops in polyploid hepatocytes. ..
  56. ncbi request reprint Quantitative isolation and in vivo imaging of malaria parasite liver stages
    Alice S Tarun
    Seattle Biomedical Research Institute, Seattle, WA 98109, USA
    Int J Parasitol 36:1283-93. 2006
    ..Intravital imaging thus reveals new, important information on the malaria parasite's transition from tissue to blood stage...