Stefan H I Kappe

Summary

Publications

  1. pmc Plasmodium falciparum PF10_0164 (ETRAMP10.3) is an essential parasitophorous vacuole and exported protein in blood stages
    Drew C MacKellar
    Seattle Biomedical Research Institute, 307 Westlake Avenue North, Suite 500, Seattle, WA 98109 5219, USA
    Eukaryot Cell 9:784-94. 2010
  2. pmc A rapid and scalable density gradient purification method for Plasmodium sporozoites
    Mark Kennedy
    Center for Mosquito Production and Malaria Infection Research, Seattle Biomedical Research Institute, 307 Westlake Avenue North, Suite 500, Seattle, WA 98109, USA
    Malar J 11:421. 2012
  3. doi request reprint That was then but this is now: malaria research in the time of an eradication agenda
    Stefan H I Kappe
    Seattle Biomedical Research Institute, Seattle, WA 98109, USA
    Science 328:862-6. 2010
  4. pmc Distinct malaria parasite sporozoites reveal transcriptional changes that cause differential tissue infection competence in the mosquito vector and mammalian host
    Sebastian A Mikolajczak
    Seattle Biomedical Research Institute, Seattle, WA 98109 5219, USA
    Mol Cell Biol 28:6196-207. 2008
  5. pmc Complete Plasmodium falciparum liver-stage development in liver-chimeric mice
    Ashley M Vaughan
    Seattle Biomedical Research Institute, Seattle, Washington 98109, USA
    J Clin Invest 122:3618-28. 2012
  6. pmc Type II fatty acid synthesis is essential only for malaria parasite late liver stage development
    Ashley M Vaughan
    Seattle Biomedical Research Institute, Seattle, WA 98109, USA
    Cell Microbiol 11:506-20. 2009
  7. pmc Targeted deletion of SAP1 abolishes the expression of infectivity factors necessary for successful malaria parasite liver infection
    Ahmed S I Aly
    Seattle Biomedical Research Institute, Seattle, WA 98109, USA
    Mol Microbiol 69:152-63. 2008
  8. pmc Total and putative surface proteomics of malaria parasite salivary gland sporozoites
    Scott E Lindner
    Malaria Program, Seattle Biomedical Research Institute, 307 Westlake Avenue North, Suite 500, Seattle, Washington 98109, USA
    Mol Cell Proteomics 12:1127-43. 2013
  9. doi request reprint A transgenic Plasmodium falciparum NF54 strain that expresses GFP-luciferase throughout the parasite life cycle
    Ashley M Vaughan
    Seattle Biomedical Research Institute, Seattle, WA, USA
    Mol Biochem Parasitol 186:143-7. 2012
  10. ncbi request reprint Protracted sterile protection with Plasmodium yoelii pre-erythrocytic genetically attenuated parasite malaria vaccines is independent of significant liver-stage persistence and is mediated by CD8+ T cells
    Alice S Tarun
    Seattle Biomedical Research Institute, Seattle, WA 98109, USA
    J Infect Dis 196:608-16. 2007

Collaborators

Detail Information

Publications43

  1. pmc Plasmodium falciparum PF10_0164 (ETRAMP10.3) is an essential parasitophorous vacuole and exported protein in blood stages
    Drew C MacKellar
    Seattle Biomedical Research Institute, 307 Westlake Avenue North, Suite 500, Seattle, WA 98109 5219, USA
    Eukaryot Cell 9:784-94. 2010
    ..We conclude that PF10_0164 is a parasitophorous vacuole protein that is essential in asexual blood stages and that does not complement P. yoelii UIS4, and it is thus likely not a functional ortholog of UIS4...
  2. pmc A rapid and scalable density gradient purification method for Plasmodium sporozoites
    Mark Kennedy
    Center for Mosquito Production and Malaria Infection Research, Seattle Biomedical Research Institute, 307 Westlake Avenue North, Suite 500, Seattle, WA 98109, USA
    Malar J 11:421. 2012
    ..However, sporozoites can only be produced in and isolated from mosquitoes, and their isolation results in large amounts of accompanying mosquito debris and contaminating microbes...
  3. doi request reprint That was then but this is now: malaria research in the time of an eradication agenda
    Stefan H I Kappe
    Seattle Biomedical Research Institute, Seattle, WA 98109, USA
    Science 328:862-6. 2010
    ..Here, we highlight some of the research into malaria parasite biology that has the potential to provide new intervention targets for antimalarial drugs and vaccines...
  4. pmc Distinct malaria parasite sporozoites reveal transcriptional changes that cause differential tissue infection competence in the mosquito vector and mammalian host
    Sebastian A Mikolajczak
    Seattle Biomedical Research Institute, Seattle, WA 98109 5219, USA
    Mol Cell Biol 28:6196-207. 2008
    ..Genes identified herein might represent targets for vector-based transmission blocking strategies (UOS genes), as well as strategies that prevent mammalian host infection (UIS genes)...
  5. pmc Complete Plasmodium falciparum liver-stage development in liver-chimeric mice
    Ashley M Vaughan
    Seattle Biomedical Research Institute, Seattle, Washington 98109, USA
    J Clin Invest 122:3618-28. 2012
    ..Thus, these mice constitute reliable models to study human LS directly in vivo and demonstrate utility for studies of LS-to-blood-stage transition of a human malaria parasite...
  6. pmc Type II fatty acid synthesis is essential only for malaria parasite late liver stage development
    Ashley M Vaughan
    Seattle Biomedical Research Institute, Seattle, WA 98109, USA
    Cell Microbiol 11:506-20. 2009
    ..Malaria parasites therefore depend on the intrinsic FAS II pathway only at one specific life cycle transition point, from liver to blood...
  7. pmc Targeted deletion of SAP1 abolishes the expression of infectivity factors necessary for successful malaria parasite liver infection
    Ahmed S I Aly
    Seattle Biomedical Research Institute, Seattle, WA 98109, USA
    Mol Microbiol 69:152-63. 2008
    ..These findings demonstrate that SAP1 is essential for liver infection possibly by functioning as a selective regulator controlling the expression of infectivity-associated parasite effector genes...
  8. pmc Total and putative surface proteomics of malaria parasite salivary gland sporozoites
    Scott E Lindner
    Malaria Program, Seattle Biomedical Research Institute, 307 Westlake Avenue North, Suite 500, Seattle, Washington 98109, USA
    Mol Cell Proteomics 12:1127-43. 2013
    ....
  9. doi request reprint A transgenic Plasmodium falciparum NF54 strain that expresses GFP-luciferase throughout the parasite life cycle
    Ashley M Vaughan
    Seattle Biomedical Research Institute, Seattle, WA, USA
    Mol Biochem Parasitol 186:143-7. 2012
    ..This parasite reporter strain will accelerate testing of interventions against pre-erythrocytic life cycle stages...
  10. ncbi request reprint Protracted sterile protection with Plasmodium yoelii pre-erythrocytic genetically attenuated parasite malaria vaccines is independent of significant liver-stage persistence and is mediated by CD8+ T cells
    Alice S Tarun
    Seattle Biomedical Research Institute, Seattle, WA 98109, USA
    J Infect Dis 196:608-16. 2007
    ..These findings have important implications for the development of a P. falciparum GAP malaria vaccine...
  11. doi request reprint An efficient strategy for gene targeting and phenotypic assessment in the Plasmodium yoelii rodent malaria model
    Sebastian A Mikolajczak
    Seattle Biomedical Research Institute, Seattle, WA 98109, USA
    Mol Biochem Parasitol 158:213-6. 2008
    ..This allows for a more rapid assessment of parasite growth in all of its developmental stages. In addition, the introduction of the fluorescent marker via the replacement strategy confers the stable integration of the marker...
  12. pmc Plasmodium yoelii sporozoites with simultaneous deletion of P52 and P36 are completely attenuated and confer sterile immunity against infection
    Mehdi Labaied
    Seattle Biomedical Research Institute, 307 Westlake Avenue North, Suite 500, Seattle, WA 98109 5219, USA
    Infect Immun 75:3758-68. 2007
    ..The study will critically guide the design of Plasmodium falciparum live attenuated malaria vaccines...
  13. doi request reprint SAP1 is a critical post-transcriptional regulator of infectivity in malaria parasite sporozoite stages
    Ahmed S I Aly
    Seattle Biomedical Research Institute, Seattle, WA 98109, USA
    Mol Microbiol 79:929-39. 2011
    ..SAP1 is therefore an appealing candidate locus for attenuation of Plasmodium falciparum...
  14. doi request reprint Disruption of the Plasmodium falciparum liver-stage antigen-1 locus causes a differentiation defect in late liver-stage parasites
    Sebastian A Mikolajczak
    Seattle Biomedical Research Institute, Seattle, WA 98109, USA
    Cell Microbiol 13:1250-60. 2011
    ..LSA-1 is the first P. falciparum protein identified to be required for this transitional stage of the parasite life cycle...
  15. pmc Plasmodium yoelii macrophage migration inhibitory factor is necessary for efficient liver-stage development
    Jessica L Miller
    Seattle Biomedical Research Institute, Seattle, Washington, USA
    Infect Immun 80:1399-407. 2012
    ..Combined, the data indicate that Plasmodium MIF is important for liver-stage development of P. yoelii, during which it is likely to play an intrinsic role in parasite development rather than modulating host immune responses to infection...
  16. pmc Preerythrocytic, live-attenuated Plasmodium falciparum vaccine candidates by design
    Kelley M VanBuskirk
    Seattle Biomedical Research Institute, Seattle, WA 98109, USA
    Proc Natl Acad Sci U S A 106:13004-9. 2009
    ..GAPs might provide a safe and reproducible platform to develop an efficacious whole-cell malaria vaccine that prevents infection at the preerythrocytic stage...
  17. ncbi request reprint Vaccination using radiation- or genetically attenuated live sporozoites
    Ashley M Vaughan
    Seattle Biomedical Research Institute, University of Washington, Seattle, WA, USA
    Methods Mol Biol 923:549-66. 2013
    ....
  18. pmc A systematic analysis of the early transcribed membrane protein family throughout the life cycle of Plasmodium yoelii
    Drew C MacKellar
    Molecular and Cellular Biology Program, University of Washington, Seattle, WA, USA
    Cell Microbiol 13:1755-67. 2011
    ..Systematic characterization of the members of the ETRAMP family reveals the diversity in importance of each family member at the interface between host and parasite throughout the developmental cycle of the malaria parasite...
  19. pmc Perturbations of Plasmodium Puf2 expression and RNA-seq of Puf2-deficient sporozoites reveal a critical role in maintaining RNA homeostasis and parasite transmissibility
    Scott E Lindner
    Seattle Biomedical Research Institute, Seattle, WA 98109, USA
    Cell Microbiol 15:1266-83. 2013
    ..These findings uncover requirements for maintaining a window of opportunity for the malaria parasite to accommodate the unpredictable moment of transmission from mosquito to mammalian host...
  20. pmc Suppression of host p53 is critical for Plasmodium liver-stage infection
    Alexis Kaushansky
    Malaria Program, Seattle Biomedical Research Institute, Seattle, WA 98109, USA
    Cell Rep 3:630-7. 2013
    ..We conclude that perturbation of the hepatocyte p53 pathway critically impacts parasite survival. Thus, host pathways might constitute potential targets for host-based antimalarial prophylaxis...
  21. doi request reprint Malaria parasite pre-erythrocytic infection: preparation meets opportunity
    Scott E Lindner
    Seattle Biomedical Research Institute, Seattle, WA 98109, USA
    Cell Microbiol 14:316-24. 2012
    ..Here we review relevant findings on how sporozoites prepare for infection of the liver and factors crucial to liver stage development as well as key host/parasite interactions...
  22. doi request reprint Subpatent infection with nucleoside transporter 1-deficient Plasmodium blood stage parasites confers sterile protection against lethal malaria in mice
    Ahmed S I Aly
    Seattle Biomedical Research Institute, Seattle, WA 98109, USA
    Cell Microbiol 12:930-8. 2010
    ..The study demonstrates that genetic manipulation provides a platform for the designed, complete attenuation of malaria parasite blood stages and suggests testing the safety and efficacy of P. falciparum NT1 knockout strains in humans...
  23. doi request reprint Plasmodium pyruvate dehydrogenase activity is only essential for the parasite's progression from liver infection to blood infection
    Ying Pei
    Seattle Biomedical Research Institute, 307 Westlake Avenue North, Suite 500, Seattle, WA 98109, USA
    Mol Microbiol 75:957-71. 2010
    ..This phenotype is similar to that observed for deletions of genes involved in FAS II elongation. The data strongly support the hypothesis that the sole role of PDH is to provide acetyl-CoA for FAS II...
  24. pmc A combined transcriptome and proteome survey of malaria parasite liver stages
    Alice S Tarun
    Seattle Biomedical Research Institute, Seattle, WA 98109, USA
    Proc Natl Acad Sci U S A 105:305-10. 2008
    ....
  25. doi request reprint Redefining the role of de novo fatty acid synthesis in Plasmodium parasites
    Alice S Tarun
    Seattle Biomedical Research Institute, Seattle, WA 98109, USA Infectious Diseases and Microbiology, University of Pittsburgh PA 15261, USA
    Trends Parasitol 25:545-50. 2009
    ..We discuss the role of fatty acid metabolism in Plasmodium and why we believe that de novo fatty acid synthesis is only required for parasite late liver-stage development...
  26. pmc Quantitative bioluminescent imaging of pre-erythrocytic malaria parasite infection using luciferase-expressing Plasmodium yoelii
    Jessica L Miller
    Seattle Biomedical Research Institute, Seattle, Washington, USA
    PLoS ONE 8:e60820. 2013
    ..Thus, this rapid, simple and noninvasive method for monitoring P. yoelii infection in the liver provides an efficient system to screen and evaluate the effects of anti-malarial interventions in vivo and in real-time...
  27. pmc Malaria parasite pre-erythrocytic stage infection: gliding and hiding
    Ashley M Vaughan
    Seattle Biomedical Research Institute, Seattle, WA 98109, USA
    Cell Host Microbe 4:209-18. 2008
    ..We discuss the factors, both parasite and host, involved in the interactions that occur during this "silent" phase of infection...
  28. pmc A dispensable Plasmodium locus for stable transgene expression
    Vanessa Y Jacobs-Lorena
    Seattle Biomedical Research Institute, Seattle, WA 98109 5219, USA
    Mol Biochem Parasitol 171:40-4. 2010
    ..yoelii wildtype parasites. Further, we show that a fluorescent transgene can be stably expressed from this site. This demonstrates that the S1 locus can be utilized for stable expression of heterologous genes in rodent malaria parasites...
  29. pmc Genetically engineered, attenuated whole-cell vaccine approaches for malaria
    Ashley M Vaughan
    Seattle Biomedical Research Institute, Seattle, WA, USA
    Hum Vaccin 6:107-13. 2010
    ....
  30. doi request reprint Malaria vaccine development: persistent challenges
    Ashley M Vaughan
    Seattle Biomedical Research Institute, 307 Westlake Avenue North, Seattle, WA 98109, USA
    Curr Opin Immunol 24:324-31. 2012
    ..Whole parasite approaches to vaccine design through attenuation as well as subunit vaccine development continue to move forward to clinical trials and are showing promising results...
  31. pmc Development of a quantitative flow cytometry-based assay to assess infection by Plasmodium falciparum sporozoites
    Alexis Kaushansky
    Seattle Biomedical Research Institute, Seattle, WA, United States
    Mol Biochem Parasitol 183:100-3. 2012
    ..This methodology will aid in assessing functional antibody responses to vaccination and novel drugs that prevent mosquito-to-man transmission of malaria...
  32. pmc Advances and challenges in malaria vaccine development
    Ruobing Wang
    Seattle Biomedical Research Institute and Department of Global Health, University of Washington, Seattle, Washington, USA
    Expert Rev Mol Med 11:e39. 2009
    ..Elimination of malaria will probably ultimately depend on the development of highly effective vaccines...
  33. ncbi request reprint L-FABP is a critical host factor for successful malaria liver stage development
    Sebastian A Mikolajczak
    Seattle Biomedical Research Institute, 307 Westlake Avenue North, Suite 500, Seattle, WA 98109 5219, USA
    Int J Parasitol 37:483-9. 2007
    ..This is the first identified direct liver stage-host cell protein interaction, providing a possible explanation for the importance of UIS3 in liver infection...
  34. ncbi request reprint Depletion of the Plasmodium berghei thrombospondin-related sporozoite protein reveals a role in host cell entry by sporozoites
    Mehdi Labaied
    Seattle Biomedical Research Institute, Seattle, WA 98109, USA
    Mol Biochem Parasitol 153:158-66. 2007
    ..Thus, TRSP is an additional TSR-containing malaria parasite protein that is mainly involved in initial infection of the mammalian host...
  35. ncbi request reprint Preerythrocytic malaria vaccine development
    Sebastian A Mikolajczak
    Seattle Biomedical Research Institute, Seattle, Washington 98109 5219, USA
    Curr Opin Infect Dis 20:461-6. 2007
    ..This review examines the potential of current preerythrocytic stage malaria vaccine approaches to reduce the global burden of malaria...
  36. pmc Malaria parasite development in the mosquito and infection of the mammalian host
    Ahmed S I Aly
    Seattle Biomedical Research Institute, Seattle, Washington 98109, USA
    Annu Rev Microbiol 63:195-221. 2009
    ..Here, we discuss what is known about the molecular and cellular basis of the developmental progression of parasites and their interactions with host tissues in the mosquito and during the early phase of mammalian infection...
  37. pmc Short-lived effector CD8 T cells induced by genetically attenuated malaria parasite vaccination express CD11c
    Laura A Cooney
    Seattle Biomedical Research Institute, Seattle, Washington, USA
    Infect Immun 81:4171-81. 2013
    ..Together, these results suggest that CD11c marks a subset of highly inflammatory, short-lived, antigen-specific effector cells, which may play an important role in eliminating infected hepatocytes. ..
  38. doi request reprint Plasmodium yoelii inhibitor of cysteine proteases is exported to exomembrane structures and interacts with yoelipain-2 during asexual blood-stage development
    Ying Pei
    Seattle Biomedical Research Institute, 307 Westlake Avenue North, Suite 500, Seattle, WA 98109, USA
    Cell Microbiol 15:1508-26. 2013
    ..These data show that ICP may be important in regulating proteolytic processes during blood-stage development, and is likely playing a role in liver stage-hepatocyte interactions at the time of exoerythrocytic merozoite release. ..
  39. pmc Real-time quantitative reverse transcription PCR for monitoring of blood-stage Plasmodium falciparum infections in malaria human challenge trials
    Sean C Murphy
    Department of Laboratory Medicine, Division of Allergy and Infectious Diseases, University of Washington Medical Center, Seattle, 98195 7110, USA
    Am J Trop Med Hyg 86:383-94. 2012
    ..7 days earlier on average than thick blood smears. This validated, internally controlled qRT-PCR method also uses a small (50 μL) sample volume requiring minimal pre-analytical handling, making it useful for clinical trials...
  40. doi request reprint Model for In Vivo Assessment of Humoral Protection against Malaria Sporozoite Challenge by Passive Transfer of Monoclonal Antibodies and Immune Serum
    Brandon K Sack
    Seattle Biomedical Research Institute, Seattle, Washington, USA
    Infect Immun 82:808-17. 2014
    ..falciparum challenge model. Together, these models constitute unique and sensitive in vivo methods to assess serum-transferable protection against Plasmodium sporozoite challenge. ..
  41. pmc Development of humanized mouse models to study human malaria parasite infection
    Ashley M Vaughan
    Seattle Biomedical Research Institute, WA 98109, USA
    Future Microbiol 7:657-65. 2012
    ..falciparum infection that will accelerate fundamental research into human parasite biology and could accelerate drug and vaccine design in the future...
  42. pmc Assessment and improvement of the Plasmodium yoelii yoelii genome annotation through comparative analysis
    Ashley Vaughan
    Seattle Biomedical Research Institute, Seattle, WA 98109, USA
    Bioinformatics 24:i383-9. 2008
    ..Unfortunately, only preliminary gene models were annotated on the partially sequenced genome, mostly by in silico gene prediction, and there has been no major improvement of the annotation since 2002...
  43. ncbi request reprint Quantitative isolation and in vivo imaging of malaria parasite liver stages
    Alice S Tarun
    Seattle Biomedical Research Institute, Seattle, WA 98109, USA
    Int J Parasitol 36:1283-93. 2006
    ..Intravital imaging thus reveals new, important information on the malaria parasite's transition from tissue to blood stage...