SS Kanwar

Summary

Affiliation: Himachal Pradesh University
Location: Shimla, India
Summary:
For the last ten years I have been working on purification of industrially important enzymes of thermophilic microorganisms (lipases) and their applications as this has been identified as a thrust area of the Department of Biotechnology at H. P. University, Shimla. The lipases(s) of a few thermophilic bacterial isolates namely B. coagulans MTCC-6375 and Bacillus sp. BTS-2, Bacillus cereus, Bacillus licheniformis, Streptomyces sp. STL-D8 and Pseudomonas aeruginosa MTCC-4713 have been fully characterized, immobilized on a variety of hydrophilic and hydrophobic synthetic and natural hydrogels and have been used for achieving hydrolysis in aqueous and esterification reactions in organic media. We are involved in synthesis of various esters (flavor/ fragrance compounds, UV blocking esters of ferulic/ cinnamic acid and precursors of pharmaceuticals and paints) in organic media using thermostable immobilized lipases sourced from Bacillus coagulans MTCC-6375 and Pseudomonas aeruginosa MTCC-4713 and other Bacillus species. We are also working on isolation, purification and characterization of extracellular cholesterol oxidase, alkaline proteases and peroxidase producing bacterial isolated and extending their potential application to develop useful applications and services. Besides industrial enzymes we are also involved in identification of anti-microbial and antilipolytic biomolecules from pteridophytes as an alternative to traditional antibiotics and antilipolytic treatment(s).
Publications:
1. Kumar A and Kanwar SS (2012). Catalytic potential of a nitrocellulose membrane-immobilized lipase in aqueous and organic media. Journal of Applied Polymer Science 124 (S1): E37-E44.
2. Kumar A, Sharma V, Sharma P and Kanwar SS (2012). Efficient immobilization of a lipase to improve its esterification potential and reusability. Chemical Papers 4: 13-23.
3. Kumar A and Kanwar SS (2012). An efficient immobilization of Streptomyces sp. STL-D8 lipase onto photo-chemically modified cellulose-based natural fibers and its application in ethyl ferulate synthesis. Trends in Carbohydrate Research 4 [In Press].
4. Bansal N, Kumari L and Kanwar SS (2012). Peroxidase and its applications. International Journal of Institutional Pharmacy and Life Sciences. www.ijipls.com (Available online) 2: 1-28.
5. Gupta SK, Rai AK, Kanwar SS and Sharma TR (2012). Comparative analysis of zinc finger proteins involved in plant disease resistance. PLoS 7(8): e42578. doi:10.1371/journal.pone.0042578
6. Thakur A, Kumar A and Kanwar SS (2012). Production of n-propyl cinnamate (musty vine amber flavor) by lipase catalysis in a non-aqueous medium. Current Biotechnology 1: 234-240.
7. Kumar A and Kanwar SS (2012). An innovative approach to immobilize lipase onto natural fiber and its application for the synthesis of 2-octyl ferulate in an organic medium Current Biotechnology 1: 241-248.
8. Jarial R, Gupta G and Kanwar SS (2012). Antimicrobial properties of some Himalayan Ferns. Himachal Pradesh University Journal 2: 136-140.
9. Sharma CK and Kanwar SS (2012). Synthesis of methyl cinnamate by Bacillus licheniformis lipase. Research J Recent Sciences 1: 68-71.
10. Sharma CK and Kanwar SS (2012). Purification of a novel thermophilic lipase from B. licheniformis MTCC-10498. ISCA Journal of Biological Sciences 1: 43-48.
11. Sharma SP, Sharma J, Kanwar SS and Chauhan VS (2012). In vitro antibacterial and antimalarial activity of dehydrophenylalanine-containing undecapeptides alone and in combination with drugs. International Journal of Antimicrobial Agents 39: 146-152.
12. Kumar A, Sharma P and Kanwar SS (2012). Lipase catalyzed esters syntheses in organic media: A Review. International Journal of Institutional Pharmacy and Life Sciences. www.ijipls.com (Available online) 2: 91-119.
13. Sharma CK and Kanwar SS (2012). Immobilization of thermocol and its hydrolytic properties. International Journal of Institutional Pharmacy and Life Sciences. www.ijipls.com (Available online) 2: 77-90.
14. Kanwar SS and Puri S (2012). Stem cells and their potential in regenerative medicine. International Journal of Institutional Pharmacy and Life Sciences. www.ijipls.com (Available online) 2: 37-58.
15. Kumari L and Kanwar SS (2012). Cholesterol oxidase and its applications. Advances in Microbiology 2: 49-65.
16. Kanwar SS (2012). Diagnostic methods for Mycobacterium tuberculosis and challenges in its detection in India. ISBN 978-953-307-3.
17. Gupta SK, Kumar A, Kanwar SS, Chand D, Singh NK and Sharma TR (2012). The single functional blast resistance gene Pi54 activates a complex defense mechanism in rice. Journal of Experimental Botany 63: 757-72.
18. Kumar A and Kanwar SS (2011). Synthesis of isopropyl ferulate using silica-immobilized lipase in an organic medium. Enzyme Research doi:10.4061/2012/718949.
19. Bala N, Kaur K, Chimni SS, Saini HS and Kanwar SS (2011). Enantioselective resolution of benzyl glycidyl ether using whole cells of Bacillus alcalophilus. Journal of Basic Microbiology 51: 1-7.
20. Sharma A, Kanwar SS and Vijh PK (2011). Molecular phylogenetic analysis of the sequences of candidate genes involved in milk production traits in riverine buffalo (Bubalus bubalis). International J of Livestock Production 2: 172-181.
21. Chandel C, Kumar A and Kanwar SS (2011). Enzymatic synthesis of butyl ferulate by silica-immobilized lipase in a non-aqueous medium. Journal of Biomaterials and Nanobiotechnology 2: 4002-408.
22. Sharma A, Kanwar SS, Tantia MS and Vijh RK (2011). Evolutionary relatedness of mammary derived kappa casein gene of riverine buffalo (Bubalus bubalis) with human and rat fibrinogen T chain. Wayamba J Animal Sciences ISSN: 2012-578X Number .
23. Sharma N, Kumar V, Sharma R, Kumari M and Kanwar SS (2011). Coordination compounds of hydroxamatooxovanadium (IV) complexes with nitrogen bases and their anti-microbial activities. Bulletin Chemical Society of Japan 84: 855-861.
24. Kanwar SS (2011). Lipases: An interesting class of biocatalysts. In proceedings: New Horizons in Microbial Biotechnology and Pharmaceutical Sciences

Publications

  1. ncbi request reprint Enhanced thermostability of silica-immobilized lipase from Bacillus coagulans BTS-3 and synthesis of ethyl propionate
    Satyendra Kumar
    Department of Biotechnology, Himachal Pradesh University, Summer Hill, Shimla 171005, India
    Acta Microbiol Immunol Hung 53:219-31. 2006
  2. ncbi request reprint Purification of a moderate thermotolerant Bacillus coagulans BTS1 lipase and its properties in a hydro-gel system
    S S Kanwar
    Department of Biotechnology, Himachal Pradesh University, Summer Hill, Shimla 171 005, India
    Acta Microbiol Immunol Hung 53:77-87. 2006
  3. doi request reprint Enzymatic synthesis of isopropyl myristate using immobilized lipase from Bacillus cereus MTCC 8372
    M L Verma
    Department of Biotechnology, Himachal Pradesh University, Summer Hill, Shimla 171 005, India
    Acta Microbiol Immunol Hung 55:327-42. 2008
  4. pmc Comparative analysis of zinc finger proteins involved in plant disease resistance
    Santosh Kumar Gupta
    National Research Centre on Plant Biotechnology, Indian Agricultural Research Institute, New Delhi, India
    PLoS ONE 7:e42578. 2012
  5. pmc Synthesis of isopropyl ferulate using silica-immobilized lipase in an organic medium
    Ashok Kumar
    Department of Biotechnology, Himachal Pradesh University, Summer Hill, Shimla 171 005, India
    Enzyme Res 2011:718949. 2011
  6. pmc Organic solvent tolerant lipases and applications
    Shivika Sharma
    Department of Biotechnology, Himachal Pradesh University, Summer Hill, Shimla 171 005, India
    ScientificWorldJournal 2014:625258. 2014
  7. pmc Peroxidase(s) in environment protection
    Neelam Bansal
    Department of Biotechnology, Himachal Pradesh University, Shimla 171 005, India
    ScientificWorldJournal 2013:714639. 2013
  8. doi request reprint Purification and characterization of a low molecular mass alkaliphilic lipase of Bacillus cereus MTCC 8372
    M L Verma
    Himachal Pradesh University, Department of Biotechnology, Shimla 171 005, India
    Acta Microbiol Immunol Hung 57:191-207. 2010
  9. doi request reprint Synthesis of ethyl acetate employing celite-immobilized lipase of Bacillus cereus MTCC 8372
    M L Verma
    Department of Biotechnology, Himachal Pradesh University, Shimla, India
    Acta Microbiol Immunol Hung 56:229-42. 2009
  10. doi request reprint Microbial lipases: at the interface of aqueous and non-aqueous media. A review
    M L Verma
    Department of Biotechnology, Himachal Pradesh University, Summer Hill, Shimla 171 005, India
    Acta Microbiol Immunol Hung 55:265-94. 2008

Collaborators

Detail Information

Publications15

  1. ncbi request reprint Enhanced thermostability of silica-immobilized lipase from Bacillus coagulans BTS-3 and synthesis of ethyl propionate
    Satyendra Kumar
    Department of Biotechnology, Himachal Pradesh University, Summer Hill, Shimla 171005, India
    Acta Microbiol Immunol Hung 53:219-31. 2006
    ..The catalytic activity of immobilized enzyme was enhanced in n-hexane and ethanol. The immobilized enzyme when used for esterification of ethanol and propionic acid showed 96% conversion in n-hexane in 12 h at 55 degrees C...
  2. ncbi request reprint Purification of a moderate thermotolerant Bacillus coagulans BTS1 lipase and its properties in a hydro-gel system
    S S Kanwar
    Department of Biotechnology, Himachal Pradesh University, Summer Hill, Shimla 171 005, India
    Acta Microbiol Immunol Hung 53:77-87. 2006
    ..Moreover, the immobilized lipase retained more than 50% of its activity after 3rd cycle of reuse...
  3. doi request reprint Enzymatic synthesis of isopropyl myristate using immobilized lipase from Bacillus cereus MTCC 8372
    M L Verma
    Department of Biotechnology, Himachal Pradesh University, Summer Hill, Shimla 171 005, India
    Acta Microbiol Immunol Hung 55:327-42. 2008
    ..The hydrogel bound lipase when repetitively used to perform esterification under optimized conditions resulted in 38.0 +/- 0.2 mM isopropyl myristate after the 3rd cycle of esterification...
  4. pmc Comparative analysis of zinc finger proteins involved in plant disease resistance
    Santosh Kumar Gupta
    National Research Centre on Plant Biotechnology, Indian Agricultural Research Institute, New Delhi, India
    PLoS ONE 7:e42578. 2012
    ..This domain is unique among all other cloned plant disease resistance genes and might play an important role in broad-spectrum nature of rice blast resistance gene Pi54...
  5. pmc Synthesis of isopropyl ferulate using silica-immobilized lipase in an organic medium
    Ashok Kumar
    Department of Biotechnology, Himachal Pradesh University, Summer Hill, Shimla 171 005, India
    Enzyme Res 2011:718949. 2011
    ..Approximately, one third of total decrease in the esterification efficacy was observed after the 5th repetitive cycle of esterification...
  6. pmc Organic solvent tolerant lipases and applications
    Shivika Sharma
    Department of Biotechnology, Himachal Pradesh University, Summer Hill, Shimla 171 005, India
    ScientificWorldJournal 2014:625258. 2014
    ..The main emphasis is to study the nature of organic solvent tolerant lipases. Also, the potential industrial applications that make lipases the biocatalysts of choice for the present and future have been presented. ..
  7. pmc Peroxidase(s) in environment protection
    Neelam Bansal
    Department of Biotechnology, Himachal Pradesh University, Shimla 171 005, India
    ScientificWorldJournal 2013:714639. 2013
    ..The review describes the sources of peroxidases, the reactions catalyzed by them, and their applications in the management of pollutants in the environment. ..
  8. doi request reprint Purification and characterization of a low molecular mass alkaliphilic lipase of Bacillus cereus MTCC 8372
    M L Verma
    Himachal Pradesh University, Department of Biotechnology, Shimla 171 005, India
    Acta Microbiol Immunol Hung 57:191-207. 2010
    ..The lipase was highly specific towards p-nitrophenyl palmitate and showed a Vmax and Km of 0.70 mmol.mg⁻¹.min⁻¹ and 32 mM for hydrolysis of pNPP...
  9. doi request reprint Synthesis of ethyl acetate employing celite-immobilized lipase of Bacillus cereus MTCC 8372
    M L Verma
    Department of Biotechnology, Himachal Pradesh University, Shimla, India
    Acta Microbiol Immunol Hung 56:229-42. 2009
    ..Batch operational stability tests indicated that immobilized lipase had retained 50% of its original catalytic activity after four consecutive batches of 15 h each...
  10. doi request reprint Microbial lipases: at the interface of aqueous and non-aqueous media. A review
    M L Verma
    Department of Biotechnology, Himachal Pradesh University, Summer Hill, Shimla 171 005, India
    Acta Microbiol Immunol Hung 55:265-94. 2008
    ..This review reports the recent development in the field ofnon-aqueous microbial lipase catalysis and factors controlling the esterification/transesterification processes in organic media...
  11. pmc Enzymatic Synthesis of Isopropyl Acetate by Immobilized Bacillus cereus Lipase in Organic Medium
    Madan Lal Verma
    Department of Biotechnology, Himachal Pradesh University, Shimla 171 005, India
    Enzyme Res 2011:919386. 2011
    ..0 mM). Ester synthesis was enhanced by water activity associated with pre-equilibrated saturated salt solution of LiCl. The immobilized lipase retained more than 50% of its activity after the 6th cycle of reuse...
  12. ncbi request reprint Methods for inhibition of residual lipase activity in colorimetric assay: a comparative study
    Shamsher Singh Kanwar
    Department of Biotechnology, Himachal Pradesh University, Summer Hill, Shimla 171 005, India
    Indian J Biochem Biophys 42:233-7. 2005
    ..cepacia and commercial grade lipolytic preparations such as lipozyme, lipolase and porcine pancreatic lipase. However, PMSF (15 mM) completely inhibited the residual activity of lipase of P. aeruginosa...
  13. ncbi request reprint Synthesis and mycobactericidal properties of metal complexes of isonicotinoyldithiocarbazic acid
    S S Kanwar
    Department of Biotechnology, Himachal Pradesh University, Shimla 171 005, India
    Biotechnol Lett 30:677-80. 2008
    ..tuberculosis. They had little cytotoxic effect on the RD cells. In contrast, the Cu complex was highly cytotoxic with a low anti-mycobacterial activity...
  14. ncbi request reprint Enhancement of ethyl propionate synthesis by poly (AAc-co-HPMA-cl-MBAm)-immobilized Pseudomonas aeruginosa MTCC-4713, exposed to Hg2+ and NH4+ ions
    S S Kanwar
    Department of Biotechnology, Himachal Pradesh University, Summer Hill, Shimla 171 005, India
    Acta Microbiol Immunol Hung 53:195-207. 2006
    ..The later showed greater specificity towards p-nitrophenyl caprylate (C 8)...
  15. ncbi request reprint Purification and properties of a novel extra-cellular thermotolerant metallolipase of Bacillus coagulans MTCC-6375 isolate
    S S Kanwar
    Department of Biotechnology, Himachal Pradesh University, Shimla 171 005, India
    Protein Expr Purif 46:421-8. 2006
    ..The lipase had a Vmax and Km of 0.44 mmol mg(-1)min(-1) and 28 mM for hydrolysis of pNPP, and 0.7 mmol mg(-1)min(-1) and 32 mM for hydrolysis of pNPC, respectively...