Norio Kagawa

Summary

Affiliation: Nagoya University
Location: Nagoya, Japan

Publications

  1. ncbi request reprint Genistein stimulates the insulin-dependent signaling pathway
    Ayumi Haneishi
    Department of Health and Nutritional Science, Faculty of Human Health Science, Matsumoto University, Matsumoto, Nagano, Japan
    Front Biosci (Elite Ed) 3:1534-40. 2011
  2. ncbi request reprint The cytochrome P450 gene family CYP157 does not contain EXXR in the K-helix reducing the absolute conserved P450 residues to a single cysteine
    Sanjeewa Rupasinghe
    Department of Cell and Developmental Biology, University of Illinois, 1201 W Gregory Dr, Urbana, IL 61801, USA
    FEBS Lett 580:6338-42. 2006
  3. ncbi request reprint Smoking cessation program and CYP2A6 polymorphism
    Shinichi Ozaki
    Department of Environmental Health, University of Occupational and Environmental Health, Kitakyushu, 807 8555, Japan
    Front Biosci 11:2590-7. 2006
  4. ncbi request reprint Raman evidence for specific substrate-induced structural changes in the heme pocket of human cytochrome P450 aromatase during the three consecutive oxygen activation steps
    Takehiko Tosha
    Okazaki Institute for Integrative Bioscience, National Institutes of Natural Sciences, Okazaki 444 8787, Japan
    Biochemistry 45:5631-40. 2006
  5. ncbi request reprint Purification and characterization of bovine steroid 21-hydroxylase (P450c21) efficiently expressed in Escherichia coli
    Miharu Arase
    Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN 37232, USA
    Biochem Biophys Res Commun 344:400-5. 2006
  6. ncbi request reprint Interaction between mitochondrial CYP27B1 and adrenodoxin: role of arginine 458 of mouse CYP27B1
    Naoko Urushino
    Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Sakyo ku, Kyoto 606 8502, Japan
    Biochemistry 45:4405-12. 2006
  7. ncbi request reprint Identification of the amino acid residue of CYP27B1 responsible for binding of 25-hydroxyvitamin D3 whose mutation causes vitamin D-dependent rickets type 1
    Keiko Yamamoto
    Institute of Biomaterials and Bioengineering and School of Biomedical Sciences, Tokyo Medical and Dental University, 2 3 10 Kanda surugadai, Chiyoda ku, Tokyo 101 0062, Japan
    J Biol Chem 280:30511-6. 2005
  8. ncbi request reprint Expression of aldehyde dehydrogenase 2 in the normal esophageal epithelium and alcohol consumption in patients with esophageal cancer
    Masaru Morita
    Second Department of Surgery, School of Medicine, University of Occupational and Environmental Health, 1 1 Iseigaoka, Kitakyushu 807 8555, Japan
    Front Biosci 10:2319-24. 2005
  9. ncbi request reprint Immunohistochemical evaluation of cytochrome P450 (CYP) and p53 in breast cancer
    Tsunehiro Oyama
    Second Department of Surgery, University of Occupational and Environmental Health, Kitakyushu, 807 8555, Japan
    Front Biosci 10:1156-61. 2005
  10. ncbi request reprint Structural diversities of active site in clinical azole-bound forms between sterol 14alpha-demethylases (CYP51s) from human and Mycobacterium tuberculosis
    Koji Matsuura
    Department of Molecular Engineering, Graduate School of Engineering, Kyoto University, Kyoto 615 8510, Japan
    J Biol Chem 280:9088-96. 2005

Collaborators

Detail Information

Publications61

  1. ncbi request reprint Genistein stimulates the insulin-dependent signaling pathway
    Ayumi Haneishi
    Department of Health and Nutritional Science, Faculty of Human Health Science, Matsumoto University, Matsumoto, Nagano, Japan
    Front Biosci (Elite Ed) 3:1534-40. 2011
    ..Our results suggest that genistein might be a useful dietary supplement to control insulin-independent diabetes mellitus by inducing the SHARP-2 expression via a bypass of the insulin-dependent signaling pathway...
  2. ncbi request reprint The cytochrome P450 gene family CYP157 does not contain EXXR in the K-helix reducing the absolute conserved P450 residues to a single cysteine
    Sanjeewa Rupasinghe
    Department of Cell and Developmental Biology, University of Illinois, 1201 W Gregory Dr, Urbana, IL 61801, USA
    FEBS Lett 580:6338-42. 2006
    ..These results indicate that the EXXR motif is not required in all CYP tertiary architectures and only a single cysteine residue, which coordinates as the fifth thiolate ligand to the P450 haem iron, is invariant in all CYPs structures...
  3. ncbi request reprint Smoking cessation program and CYP2A6 polymorphism
    Shinichi Ozaki
    Department of Environmental Health, University of Occupational and Environmental Health, Kitakyushu, 807 8555, Japan
    Front Biosci 11:2590-7. 2006
    ..Although treatment of nicotine patch is efficacious to smoking cessation, cases with CYP2A619934/19934 might be more sensitive to nicotine adverse effects and more difficult to quit smoking once they have smoking habit...
  4. ncbi request reprint Raman evidence for specific substrate-induced structural changes in the heme pocket of human cytochrome P450 aromatase during the three consecutive oxygen activation steps
    Takehiko Tosha
    Okazaki Institute for Integrative Bioscience, National Institutes of Natural Sciences, Okazaki 444 8787, Japan
    Biochemistry 45:5631-40. 2006
    ..We, therefore, propose that the substrates can regulate the formation of alternative reaction intermediates by modulating the structure on both the heme distal and proximal sites in P450arom...
  5. ncbi request reprint Purification and characterization of bovine steroid 21-hydroxylase (P450c21) efficiently expressed in Escherichia coli
    Miharu Arase
    Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN 37232, USA
    Biochem Biophys Res Commun 344:400-5. 2006
    ..Truncation of the membrane anchor increases solubility of P450c21 facilitating expression of this protein in E. coli yielding sufficient quantities for both biochemical and biophysical studies...
  6. ncbi request reprint Interaction between mitochondrial CYP27B1 and adrenodoxin: role of arginine 458 of mouse CYP27B1
    Naoko Urushino
    Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Sakyo ku, Kyoto 606 8502, Japan
    Biochemistry 45:4405-12. 2006
    ..These results suggest that ADX functions as an effector for the oxygen transfer reaction in addition to being an electron donor for CYP27B1...
  7. ncbi request reprint Identification of the amino acid residue of CYP27B1 responsible for binding of 25-hydroxyvitamin D3 whose mutation causes vitamin D-dependent rickets type 1
    Keiko Yamamoto
    Institute of Biomaterials and Bioengineering and School of Biomedical Sciences, Tokyo Medical and Dental University, 2 3 10 Kanda surugadai, Chiyoda ku, Tokyo 101 0062, Japan
    J Biol Chem 280:30511-6. 2005
    ..This is the first report that shows a critical amino acid residue recognizing the 25-hydroxyl group of the vitamin D3...
  8. ncbi request reprint Expression of aldehyde dehydrogenase 2 in the normal esophageal epithelium and alcohol consumption in patients with esophageal cancer
    Masaru Morita
    Second Department of Surgery, School of Medicine, University of Occupational and Environmental Health, 1 1 Iseigaoka, Kitakyushu 807 8555, Japan
    Front Biosci 10:2319-24. 2005
    ..To our knowledge, this is the first study demonstrating the induction of ALDH2 in the esophagus by ethanol consumption...
  9. ncbi request reprint Immunohistochemical evaluation of cytochrome P450 (CYP) and p53 in breast cancer
    Tsunehiro Oyama
    Second Department of Surgery, University of Occupational and Environmental Health, Kitakyushu, 807 8555, Japan
    Front Biosci 10:1156-61. 2005
    ..Since CYP3A is involved in the conversion of tamoxifen to its metabolites, the variation of the CYP3A expression in breast cancer tissues among ethnic groups might cause differences in the efficacy of tamoxifen...
  10. ncbi request reprint Structural diversities of active site in clinical azole-bound forms between sterol 14alpha-demethylases (CYP51s) from human and Mycobacterium tuberculosis
    Koji Matsuura
    Department of Molecular Engineering, Graduate School of Engineering, Kyoto University, Kyoto 615 8510, Japan
    J Biol Chem 280:9088-96. 2005
    ....
  11. ncbi request reprint Metabolism of vitamin D3 by cytochromes P450
    Toshiyuki Sakaki
    Biotechnology Research Center, Faculty of Engineering, Toyama Prefectural University, Kosugi, Toyama 939 0398, Japan
    Front Biosci 10:119-34. 2005
    ....
  12. ncbi request reprint Tissue-distribution of aldehyde dehydrogenase 2 and effects of the ALDH2 gene-disruption on the expression of enzymes involved in alcohol metabolism
    Tsunehiro Oyama
    Department of Environmental Health, University of Occupational and Environmental Health, Kitakyushu, 807 8555, Japan
    Front Biosci 10:951-60. 2005
    ..This observation suggests that a metabolite(s) of Aldh2 might down-regulate the expression of Cyp2e1 gene...
  13. ncbi request reprint Tetracycline protects myocardium against ischemic injury
    Norio Kagawa
    Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN 37252 0146, USA
    Front Biosci 10:608-19. 2005
    ..This study is also important for providing new insights into the non-antimicrobial effects of tetracycline and its derivatives...
  14. ncbi request reprint Purification and characterization of mouse CYP27B1 overproduced by an Escherichia coli system coexpressing molecular chaperonins GroEL/ES
    Eriko Uchida
    Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Kitashirakawa, Oiwake cho, Sakyo ku, Kyoto 606 8502, Japan
    Biochem Biophys Res Commun 323:505-11. 2004
    ..Based on these results and the fact that human CYP27A1 and Streptomyces CYP105A1 also convert vitamin D3 to 1alpha,25(OH)D3, 1alpha-hydroxylation, and 25-hydroxylation of vitamin D3 appear to be closely linked together...
  15. ncbi request reprint Cytochrome P450 expression (CYP) in non-small cell lung cancer
    Tsunehiro Oyama
    Second Department of Surgery, University of Occupational and Environmental Health, Kitakyushu, 807 8555, Japan
    Front Biosci 12:2299-308. 2007
    ..Therefore, the spectrum of CYP proteins in lung cancer could be useful for changing the present "order-made" therapy to the "tailor-made" therapy...
  16. ncbi request reprint BS69, a corepressor interacting with ZHX1, is a bifunctional transcription factor
    Hiroko Ogata-Kawata
    Department of Biochemistry, Faculty of Medical Sciences, University of Fukui, Fukui 910 1193, Japan
    Front Biosci 12:1911-26. 2007
    ..Therefore, our results suggest that BS69 may function either as a transcriptional repressor or as a transcriptional activator depending on its regulatory partner...
  17. pmc Lung cancer andCYP1A1 orGSTM1 polymorphisms
    Tsunehiro Oyama
    Departments of Environmental Health, University of Occupational and Environmental Health, 807, Kitakyushu, Japan
    Environ Health Prev Med 7:230-4. 2003
    ..In this review, we will summarize the polymorphisms in theGSTM1 andCYP1A1 genes and their relation to lung cancer susceptibility...
  18. doi request reprint The CYPome of Sorangium cellulosum So ce56 and identification of CYP109D1 as a new fatty acid hydroxylase
    Yogan Khatri
    Department of Biochemistry, Saarland University, Campus B2 2, 66123 Saarbrucken, Germany
    Chem Biol 17:1295-305. 2010
    ..The kinetic parameters for the product hydroxylation were derived...
  19. ncbi request reprint Effects of acetaldehyde inhalation in mitochondrial aldehyde dehydrogenase deficient mice (Aldh2-/-)
    Tsunehiro Oyama
    Department of Environmental Health, School of Medicine, University of Occupational and Environmental Health, Kitakyushu, Fukuoka 807 8555, Japan
    Front Biosci (Elite Ed) 2:1344-54. 2010
    ..Acetaldehyde inhalation may cause a high risk in nasal epithelium and lung cancers for individuals with inactive ALDH2...
  20. ncbi request reprint EGFR gene alterations as a prognostic biomarker in advanced esophageal squamous cell carcinoma
    Kazuhiro Kaneko
    Division of Digestive Endoscopy Gastrointestinal Oncology, National Cancer Center Hospital East, Chiba, 277 8577, Japan
    Front Biosci 15:65-72. 2010
    ..These results suggest that the EGFR SNP at codon 787 of exon 20 determined in pretreatment biopsy specimens may be a clinically useful biomarker for predicting the prognosis of ESCC patients...
  21. ncbi request reprint Hazardous environmental factors enhance impairment of liver function in HBV and HCV hepatitis
    Yasunori Yashima
    Department of Environmental Health, University of Occupational and Environmental Health, Kitakyushu, 807 8555, Japan
    Front Biosci (Landmark Ed) 14:4071-8. 2009
    ..Therefore, hazardous working conditions have to be carefully considered in the progress of chronic liver disease in workers infected with HBV and HCV...
  22. ncbi request reprint ZHX2 and ZHX3 repress cancer markers in normal hepatocytes
    Kazuya Yamada
    Department of Biochemistry, Faculty of Medical Sciences, University of Fukui, Fukui 910 1193, Japan
    Front Biosci (Landmark Ed) 14:3724-32. 2009
    ....
  23. ncbi request reprint Expression of aromatase CYP19 and its relationship with parameters in NSCLC
    Tsunehiro Oyama
    Second Department of Surgery and Department of Environmental Health, University of Occupational and Environmental Health, Kitakyushu, 807 8555, Japan
    Front Biosci (Landmark Ed) 14:2285-92. 2009
    ..The results suggest that the aromatase inhibitors might be useful for the management of non-small cell lung cancer in postmenopausal women...
  24. pmc Aromatase is phosphorylated in situ at serine-118
    Todd W Miller
    Department of Medicine, Vanderbilt Ingram Comprehensive Cancer Center, Vanderbilt University School of Medicine, Nashville, TN 37232, USA
    J Steroid Biochem Mol Biol 112:95-101. 2008
    ..Our findings suggest that phosphorylation of S118 may decrease aromatase activity, presenting a mechanism whereby kinase signaling may modulate estrogen production and hormone balance...
  25. ncbi request reprint Expression of cytochrome P450 in non-small cell lung cancer
    Tsunehiro Oyama
    Second Department of Surgery, University of Occupational and Environmental Health, Kitakyushu, 807 8555, Japan
    Front Biosci 13:5787-93. 2008
    ..The understanding of the local expression of CYP enzymes in tumor tissues is important in the development of better treatment for lung cancer and a standardized treatment, tailor-made, for individual patients...
  26. pmc The ferrous-oxy complex of human aromatase
    Yelena V Grinkova
    Department of Biochemistry, Center for Biophysics and Computational Biology, University of Illinois, Urbana, IL 61801, USA
    Biochem Biophys Res Commun 372:379-82. 2008
    ....
  27. doi request reprint Purification and functional characterization of human 11beta hydroxylase expressed in Escherichia coli
    Andy Zöllner
    Department of Biochemistry, Saarland University, Saarbrucken, Germany
    FEBS J 275:799-810. 2008
    ..In addition, we performed CD measurements to obtain information about the structure of hCYP11B1...
  28. ncbi request reprint Interaction between substrate and oxygen ligand responsible for effective O-O bond cleavage in bovine cytochrome P450 steroid 21-hydroxylase proved by Raman spectroscopy
    Takehiko Tosha
    Okazaki Institute for Integrative Bioscience, National Institutes of Natural Sciences, Okazaki, Aichi 444 8787, Japan
    J Biol Chem 283:3708-17. 2008
    ..In the hydrogen-bonded species, the substrate changes the geometry of the FeOO moiety, thereby performing the hydroxylation reaction more effectively in 17alpha-hydroxyprogesterone than in progesterone...
  29. ncbi request reprint Increased cytochrome P450 and aryl hydrocarbon receptor in bronchial epithelium of heavy smokers with non-small cell lung carcinoma carries a poor prognosis
    Tsunehiro Oyama
    Department of Environmental Health, University of Occupational and Environmental Health, Yahatanishi ku, Kitakyushu, Japan
    Front Biosci 12:4497-503. 2007
    ..5%) than those without (12.2%) mutation. Since in patients with early NSCLC, CYP positivity showed a close correlation with a poor survival (p less than 0.01), expression of CYP in bronchial epithelium has a prognostic potential...
  30. ncbi request reprint Characterization of stable human aromatase expressed in E. coli
    Norio Kagawa
    Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN 37232 0146, USA
    Steroids 69:235-43. 2004
    ..The highly stable and active P450arom NmA264R sets the stope for detailed structure/function analyses of this important member of the P450 superfamily...
  31. ncbi request reprint Expression of cytochrome P450 in tumor tissues and its association with cancer development
    Tsunehiro Oyama
    Department of Environmental Health, School of Medicine, University of Occupational and Environmental Health, Kitakyushu 807 8555, Japan
    Front Biosci 9:1967-76. 2004
    ..In this review, we summarize the association of CYP expression in cancer tissues with carcinogenesis and cancer treatment...
  32. ncbi request reprint Escherichia coli flavodoxin sepharose as an affinity resin for cytochromes P450 and use to identify a putative cytochrome P450c17/3beta-hydroxysteroid dehydrogenase interaction
    C M Jenkins
    Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 0146, USA
    Arch Biochem Biophys 347:93-102. 1997
    ..From these results, we propose that 3beta-HSD and P450c17 may associate, perhaps as part of a steroidogenic complex, in the endoplasmic reticulum...
  33. ncbi request reprint cAMP-dependent transactivation involving the homeodomain protein Pbx1
    A Ogo
    Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, Tennessee, 37232 0146, USA
    Arch Biochem Biophys 338:193-200. 1997
    ....
  34. ncbi request reprint Expression and purification of functional human 17 alpha-hydroxylase/17,20-lyase (P450c17) in Escherichia coli. Use of this system for study of a novel form of combined 17 alpha-hydroxylase/17,20-lyase deficiency
    T Imai
    Department of Biochemistry and Obstetrics, University of Texas Southwestern Medical Center, Dallas 75235
    J Biol Chem 268:19681-9. 1993
    ..Upon expression of these mutant proteins in E. coli, the Tyr64 mutant has 15% of the wild type 17 alpha-hydroxylase activity, whereas the Ile112 duplication shows no activity, results consistent with the observed clinical phenotype...
  35. ncbi request reprint cAMP-dependent and tissue-specific expression of genes encoding steroidogenic enzymes in bovine luteal and granulosa cells in primary culture
    M E Lauber
    Cecil H and Ida Green Center for Reproductive Biology Sciences, University of Texas Southwestern Medical Center, Dallas 75235 9051
    Mol Cell Endocrinol 93:227-33. 1993
    ..Northern blot analysis of primary cultures of bovine luteal and granulosa cells revealed that bCYP17 and bCYP21B are not expressed in control and forskolin-treated cultures.(ABSTRACT TRUNCATED AT 250 WORDS)..
  36. ncbi request reprint A cAMP-regulatory sequence (CRS1) of CYP17 is a cellular target for the homeodomain protein Pbx1
    N Kagawa
    Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 0146
    J Biol Chem 269:18716-9. 1994
    ..These results identify the CRS1 of bovine CYP17 as a cellular target for Pbx1 and suggest that one role of this homeodomain protein is in the regulation of steroidogenesis and subsequently sexual development...
  37. ncbi request reprint The role of cytochrome b5 in the biosynthesis of androgens by human P450c17
    M Katagiri
    Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 0146
    Arch Biochem Biophys 317:343-7. 1995
    ....
  38. ncbi request reprint Protein kinase A-dependent transactivation by the E2A-Pbx1 fusion protein
    A Ogo
    Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 0146, USA
    J Biol Chem 270:25340-3. 1995
    ..Thus, it seems plausible that arrest in the pre-B stage leading to pre-B ALL includes cAMP-dependent activation of E2A-Pbx1...
  39. ncbi request reprint Active-site topology of bovine cholesterol side-chain cleavage cytochrome P450 (P450scc) and evidence for interaction of tyrosine 94 with the side chain of cholesterol
    I A Pikuleva
    Department of Biochemistry, Vanderbilt University, School of Medicine, Nashville, Tennessee 37232 0146, USA
    Arch Biochem Biophys 322:189-97. 1995
    ....
  40. ncbi request reprint Structural analysis of cloned cDNAs for polycyclic hydrocarbon-inducible forms of rabbit liver microsomal cytochrome P-450
    N Kagawa
    Institute for Protein Research, Osaka University
    J Biochem 101:1471-9. 1987
    ....
  41. ncbi request reprint Deduced amino acid sequence of heme binding region of chicken cholesterol side chain cleavage cytochrome P450
    P A Mathew
    Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas 75235
    Protein Seq Data Anal 3:323-5. 1990
    ..The heme binding region of the avian P450scc shows 76% homology with the heme binding regions of rat and human P450scc and 81% homology with that of bovine P450scc...
  42. ncbi request reprint cAMP-dependent transcription of the human CYP21B (P-450C21) gene requires a cis-regulatory element distinct from the consensus cAMP-regulatory element
    N Kagawa
    Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas 75235
    J Biol Chem 265:11299-305. 1990
    ....
  43. ncbi request reprint Evidence that an adrenal-specific nuclear protein regulates the cAMP responsiveness of the human CYP21B (P450C21) gene
    N Kagawa
    Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas 75235
    J Biol Chem 266:11199-204. 1991
    ....
  44. ncbi request reprint Purification and characterization of a transcription factor which appears to regulate cAMP responsiveness of the human CYP21B gene
    N Kagawa
    Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas 75235
    J Biol Chem 267:25213-9. 1992
    ..These results indicate that ASP is a primary transcription factor for the cAMP-dependent regulation of the human CYP21B gene...
  45. ncbi request reprint The homeodomain protein Pbx1 is involved in cAMP-dependent transcription of human CYP17
    A Ogo
    Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 0146, USA
    Arch Biochem Biophys 348:226-31. 1997
    ....
  46. ncbi request reprint Members of the meis1 and pbx homeodomain protein families cooperatively bind a cAMP-responsive sequence (CRS1) from bovine CYP17
    L J Bischof
    Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 0146, USA
    J Biol Chem 273:7941-8. 1998
    ..Thus, CYP17 CRS1 is a transcriptional regulatory element containing both Pbx and Meis1 binding sites, which permit these two homeodomain proteins to bind and potentially regulate cAMP-dependent transcription through this sequence...
  47. ncbi request reprint [Expression of foreign proteins in E. coli: through the expression of P450s]
    Norio Kagawa
    Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN 37232 0146, USA
    Seikagaku 75:279-84. 2003
  48. ncbi request reprint Expression of human aromatase (CYP19) in Escherichia coli by N-terminal replacement and induction of cold stress response
    Norio Kagawa
    Department of Biochemistry, School of Medicine, Vanderbilt University, Nashville, TN 37232 0146, USA
    Steroids 68:205-9. 2003
    ..9 nmol/min/nmol P450. Our results are important to provide large quantities of human P450arom as an active form for structure-function studies...
  49. ncbi request reprint Transcriptional activation of human CYP17 in H295R adrenocortical cells depends on complex formation among p54(nrb)/NonO, protein-associated splicing factor, and SF-1, a complex that also participates in repression of transcription
    Marion B Sewer
    Department of Biochemistry and Center in Toxicology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 0146, USA
    Endocrinology 143:1280-90. 2002
    ..Our studies lend support to the idea that the balance between transcriptional activation and repression is essential in the control of adrenocortical steroid hormone biosynthesis...
  50. ncbi request reprint Osmotic stress induced by carbohydrates enhances expression of foreign proteins in Escherichia coli
    N Kagawa
    Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 0146, USA
    Arch Biochem Biophys 393:290-6. 2001
    ..coli via the induction of the osmotic stress response. The results suggest the important role of the osmotic stress response in posttranscriptional processes required for producing functional proteins...
  51. ncbi request reprint Importance of a proline-rich sequence in the amino-terminal region for correct folding of mitochondrial and soluble microbial p450s
    K Kusano
    Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN 37232 0146, USA
    J Biochem 129:271-7. 2001
    ..Furthermore, we predict that the importance of the PR in P450 folding is to reduce the tendency of the polypeptide to misfold prior to heme binding...
  52. ncbi request reprint Microsomal p450s use specific proline-rich sequences for efficient folding, but not for maintenance of the folded structure
    K Kusano
    Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN 37232 0146, USA
    J Biochem 129:259-69. 2001
    ..We conclude that PR is important for directing the folding pathway leading to the functional P450, but not for maintaining the functional form...
  53. ncbi request reprint Three zinc finger nuclear proteins, Sp1, Sp3, and a ZBP-89 homologue, bind to the cyclic adenosine monophosphate-responsive sequence of the bovine adrenodoxin gene and regulate transcription
    P Y Cheng
    Department of Biochemistry, Medical School, Vanderbilt University, Nashville, Tennessee 37232 0146, USA
    Biochemistry 39:4347-57. 2000
    ..Sp1 and Sp3 confer basal transcriptional activities, yet only Sp1 confers cAMP-responsive activity. ZBP-89 represses basal transcriptional activity...
  54. ncbi request reprint Protein synthesis inhibitors and ethanol selectively enhance heterologous expression of P450s and related proteins in Escherichia coli
    K Kusano
    Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, Tennessee, 37232 0146, USA
    Arch Biochem Biophys 367:129-36. 1999
    ..coli. This study also indicates that protein synthesis inhibitors associated with the cold shock response may act as protein synthesis enhancers under certain conditions...
  55. ncbi request reprint Biochemical diversity of peptide-hormone-dependent regulation of steroidogenic P450s
    N Kagawa
    Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 0146, USA
    Drug Metab Rev 31:333-42. 1999
  56. ncbi request reprint The bovine CYP17 promoter contains a transcriptional regulatory element cooperatively bound by tale homeodomain proteins
    L J Bischof
    Department of Biochemistry, Vanderbilt University, Nashville, TN, USA
    Endocr Res 24:489-95. 1998
    ..A current model for the function of these proteins in CYP17 regulation is that they may enhance the cAMP response through the downstream SF-1 binding site...
  57. ncbi request reprint Structure/function relationship of CYP11B1 associated with Dahl's salt-resistant rats--expression of rat CYP11B1 and CYP11B2 in Escherichia coli
    Y Nonaka
    Department of Basic Laboratory Sciences, Osaka University Medical School, Japan
    Eur J Biochem 258:869-78. 1998
    ..A decrease in these enzyme activities may be responsible for the normotension of the DR rats when fed a high-salt diet...
  58. ncbi request reprint [The regulation of steroidogenesis by 17 alpha-hydroxylase/17,20-lyase (P450c17)]
    M Katagiri
    Division of Natural Science, Osaka Kyoiku University, Japan
    Nihon Yakurigaku Zasshi 112:43-50. 1998
    ..In addition, the age-dependent expression of P450c17 in immature rat livers is also discussed...
  59. ncbi request reprint Evidence that immature rat liver is capable of participating in steroidogenesis by expressing 17alpha-hydroxylase/17,20-lyase P450c17
    M Katagiri
    Division of Natural Science, Osaka Kyoiku University, Kashiwara, Japan
    J Steroid Biochem Mol Biol 64:121-8. 1998
    ....
  60. ncbi request reprint Distinct biochemical mechanisms for cAMP-dependent transcription of CYP17 and CYP21
    U M Zanger
    Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas 75235
    FASEB J 6:719-23. 1992
    ..Thus the ACTH-dependent transcription of these two genes, which have a common evolutionary origin and are coordinately expressed in the adrenal cortex, involves distinct biochemical mechanisms...
  61. doi request reprint Efficient expression of human aromatase (CYP19) in E. coli
    Norio Kagawa
    Office of Global COE, Nagoya University School of Medicine, Nagoya 466 8550, Japan
    Methods Mol Biol 705:109-22. 2011
    ..Therefore, the expression methods for the two forms of human aromatase as well as a method for the determination of the reduced CO-difference spectrum will be described...