Russell Jones

Summary

Affiliation: NIBSC
Location: Potters Bar, UK
Summary:
Senior Scientist

Publications

  1. doi request reprint A functional dual-coated (FDC) microtiter plate method to replace the botulinum toxin LD50 test
    Yvonne Y B Liu
    Division of Bacteriology, National Institute for Biological Standards and Control, Health Protection Agency, South Mimms, Hertfordshire, UK
    Anal Biochem 425:28-35. 2012
  2. pmc Isolation of a nanomolar scFv inhibiting the endopeptidase activity of botulinum toxin A, by single-round panning of an immune phage-displayed library of macaque origin
    Siham Chahboun
    Unité de biotechnologie des anticorps, et des toxines, Departement de Microbiologie, Institut de recherche biomédicale des armées IRBA CRSSA, 24 Avenue des Maquis du Gresivaudan, BP 87, 38702 La Tronche Cedex, France
    BMC Biotechnol 11:113. 2011
  3. doi request reprint Phrenic nerve-hemidiaphragm as a highly sensitive replacement assay for determination of functional botulinum toxin antibodies
    C Rasetti-Escargueil
    Division of Bacteriology and Biostatistics Section, National Institute for Biological Standards and Control NIBSC, Health Protection Agency HPA, South Mimms, Potters Bar, Hertfordshire EN6 3QG, UK
    Toxicon 57:1008-16. 2011
  4. doi request reprint Release of proteolytic activity following reduction in therapeutic human serum albumin containing products: detection with a new neoepitope endopeptidase immunoassay
    Russell G A Jones
    National Institute for Biological Standards and Control, Health Protection Agency, Division of Bacteriology, South Mimms, Potters Bar, Hertfordshire EN6 3QG, UK
    J Pharm Biomed Anal 54:74-80. 2011
  5. pmc Vaccination of rabbits with an alkylated toxoid rapidly elicits potent neutralizing antibodies against botulinum neurotoxin serotype B
    Daniel M Held
    Biosciences Division, SRI International, 333 Ravenswood Ave, Menlo Park, CA 94025, USA
    Clin Vaccine Immunol 17:930-6. 2010
  6. doi request reprint Measurement of botulinum types A, B and E neurotoxicity using the phrenic nerve-hemidiaphragm: improved precision with in-bred mice
    C Rasetti-Escargueil
    Division of Bacteriology, National Institute for Biological Standards and Control Blanche Lane, South Mimms, Potters Bar, Hertfordshire, EN6 3QG, UK
    Toxicon 53:503-11. 2009
  7. doi request reprint New highly specific botulinum type C1 endopeptidase immunoassays utilising SNAP25 or Syntaxin substrates
    Russell G A Jones
    Division of Bacteriology, National Institute for Biological Standards and Control, Hertfordshire, UK
    J Immunol Methods 343:21-7. 2009
  8. doi request reprint An improved method for development of toxoid vaccines and antitoxins
    Russell G A Jones
    Division of Bacteriology, National Institute for Biological Standards and Control, Potters Bar, Hertfordshire, UK
    J Immunol Methods 337:42-8. 2008
  9. ncbi request reprint Development of improved SNAP25 endopeptidase immuno-assays for botulinum type A and E toxins
    R G A Jones
    Division of Bacteriology, National Institute for Biological Standards and Control, South Mimms, Potters Bar, Hertfordshire, EN6 3QG, UK
    J Immunol Methods 329:92-101. 2008
  10. ncbi request reprint Botulinum type A toxin neutralisation by specific IgG and its fragments: a comparison of mouse systemic toxicity and local flaccid paralysis assays
    R G A Jones
    Division of Bacteriology, National Institute for Biological Standards and Control, South Mimms, Potters Bar, Hertfordshire, EN6 3QG, UK
    Toxicon 48:246-54. 2006

Collaborators

Detail Information

Publications18

  1. doi request reprint A functional dual-coated (FDC) microtiter plate method to replace the botulinum toxin LD50 test
    Yvonne Y B Liu
    Division of Bacteriology, National Institute for Biological Standards and Control, Health Protection Agency, South Mimms, Hertfordshire, UK
    Anal Biochem 425:28-35. 2012
    ..This is the first known immunobiochemical assay that correlates with in vivo potency and provides a realistic alternative...
  2. pmc Isolation of a nanomolar scFv inhibiting the endopeptidase activity of botulinum toxin A, by single-round panning of an immune phage-displayed library of macaque origin
    Siham Chahboun
    Unité de biotechnologie des anticorps, et des toxines, Departement de Microbiologie, Institut de recherche biomédicale des armées IRBA CRSSA, 24 Avenue des Maquis du Gresivaudan, BP 87, 38702 La Tronche Cedex, France
    BMC Biotechnol 11:113. 2011
    ..In the present study, a phage-displayed library was constructed from a macaque (Macaca fascicularis) hyper-immunized with BoNTA/L in order to isolate scFvs inhibiting BoNT/A endopeptidase activity for clinical use...
  3. doi request reprint Phrenic nerve-hemidiaphragm as a highly sensitive replacement assay for determination of functional botulinum toxin antibodies
    C Rasetti-Escargueil
    Division of Bacteriology and Biostatistics Section, National Institute for Biological Standards and Control NIBSC, Health Protection Agency HPA, South Mimms, Potters Bar, Hertfordshire EN6 3QG, UK
    Toxicon 57:1008-16. 2011
    ..964, respectively). These findings confirm that the mouse nerve-diaphragm preparation can provide a functional ex vivo replacement assay for specific, sensitive and precise assessment of toxin and antitoxin activity...
  4. doi request reprint Release of proteolytic activity following reduction in therapeutic human serum albumin containing products: detection with a new neoepitope endopeptidase immunoassay
    Russell G A Jones
    National Institute for Biological Standards and Control, Health Protection Agency, Division of Bacteriology, South Mimms, Potters Bar, Hertfordshire EN6 3QG, UK
    J Pharm Biomed Anal 54:74-80. 2011
    ..Taken together, these findings indicate a new intrinsic proteolytic activity of the albumin molecule revealed under reducing conditions as the source of the unexpected Arg-Ala cleaving activity...
  5. pmc Vaccination of rabbits with an alkylated toxoid rapidly elicits potent neutralizing antibodies against botulinum neurotoxin serotype B
    Daniel M Held
    Biosciences Division, SRI International, 333 Ravenswood Ave, Menlo Park, CA 94025, USA
    Clin Vaccine Immunol 17:930-6. 2010
    ..Overall, the immunization of rabbits with alkylated BoNT/B toxoid appears to have induced a neutralizing immune response more rapid and more potent than the responses generated by vaccination with formaldehyde toxoid or rHc preparations...
  6. doi request reprint Measurement of botulinum types A, B and E neurotoxicity using the phrenic nerve-hemidiaphragm: improved precision with in-bred mice
    C Rasetti-Escargueil
    Division of Bacteriology, National Institute for Biological Standards and Control Blanche Lane, South Mimms, Potters Bar, Hertfordshire, EN6 3QG, UK
    Toxicon 53:503-11. 2009
    ..These findings indicate that when fully optimised the mouse nerve-diaphragm preparation can provide a functional in vitro model for accurate and reproducible assessment of toxin activity...
  7. doi request reprint New highly specific botulinum type C1 endopeptidase immunoassays utilising SNAP25 or Syntaxin substrates
    Russell G A Jones
    Division of Bacteriology, National Institute for Biological Standards and Control, Hertfordshire, UK
    J Immunol Methods 343:21-7. 2009
    ..These new immunoassays will therefore provide highly specific tools for monitoring botulinum toxin light chain endopeptidase activity and serotype identity...
  8. doi request reprint An improved method for development of toxoid vaccines and antitoxins
    Russell G A Jones
    Division of Bacteriology, National Institute for Biological Standards and Control, Potters Bar, Hertfordshire, UK
    J Immunol Methods 337:42-8. 2008
    ..Toxoid produced by the new method should substantially improve both antitoxin and vaccine production and be applicable to other toxins and immunogens...
  9. ncbi request reprint Development of improved SNAP25 endopeptidase immuno-assays for botulinum type A and E toxins
    R G A Jones
    Division of Bacteriology, National Institute for Biological Standards and Control, South Mimms, Potters Bar, Hertfordshire, EN6 3QG, UK
    J Immunol Methods 329:92-101. 2008
    ..The principle of using a detecting antibody to a substrate sequence buried within the native substrates alpha-helix may be further expanded to other specific enzyme cleavage reactions in the future...
  10. ncbi request reprint Botulinum type A toxin neutralisation by specific IgG and its fragments: a comparison of mouse systemic toxicity and local flaccid paralysis assays
    R G A Jones
    Division of Bacteriology, National Institute for Biological Standards and Control, South Mimms, Potters Bar, Hertfordshire, EN6 3QG, UK
    Toxicon 48:246-54. 2006
    ..These findings show that a local flaccid paralysis assay provides a less time consuming and more humane alternative to the lethal assay for the potency testing of botulinum IgG and F(ab')(2) antitoxins...
  11. ncbi request reprint A review of WHO International Standards for botulinum antitoxins
    R G A Jones
    Division of Bacteriology, National Institute for Biological Standards and Control, Potters Bar, Hertfordshire EN6 3QG, UK
    Biologicals 34:223-6. 2006
    ..We describe here the production and characterization of stable freeze dried potential candidate replacements along with a new prospective first IS for type G antitoxin. Available toxin A reference preparations are also briefly reviewed...
  12. ncbi request reprint Detection of antibodies against botulinum toxins
    Dorothea Sesardic
    Division of Bacteriology, National Institute for Biological Standards and Control, Potters Bar, Hertfordshire, United Kingdom
    Mov Disord 19:S85-91. 2004
    ..Most recent promising developments are focused on cellular assays utilising primary rat embryonic cord cells or more conveniently in vitro differentiated established cell lines such as human neuroblastoma cells...
  13. ncbi request reprint A protocol for 'enhanced pepsin digestion': a step by step method for obtaining pure antibody fragments in high yield from serum
    R G A Jones
    Division of Bacteriology, National Institute for Biological Standards and Control NIBSC, Blanche Lane, South Mimms, Potters Bar, Hertfordshire EN6 3QG, UK
    J Immunol Methods 275:239-50. 2003
    ..If required, a mild reducing agent may be used finally to create Fab fragments...
  14. ncbi request reprint Enhanced pepsin digestion: a novel process for purifying antibody F(ab')(2) fragments in high yield from serum
    R G A Jones
    Department of Clinical Biochemistry, Barts and The London, Queen Mary s School of Medicine and Dentistry, UK
    J Immunol Methods 263:57-74. 2002
    ..3 g F(ab')(2)/l serum. This novel, high yield method for processing serum to highly pure F(ab')(2) avoids salt precipitation and centrifugation and should be suitable for large-scale production...
  15. doi request reprint Use of a new functional dual coating (FDC) assay to measure low toxin levels in serum and food samples following an outbreak of human botulism
    Russell G A Jones
    1Division of Bacteriology, National Institute for Biological Standards and Control, Health Protection Agency, South Mimms, Hertfordshire, UK
    J Med Microbiol 62:828-35. 2013
    ..32 LD50 per ml extract. The FDC assay provides a real alternative for public health laboratories to unambiguously confirm all cases of type A botulism and, due to its sensitivity, a promising new tool in toxin pharmacokinetic studies...