Research Topics
Genomes and Genes
| Fumihiko TakeshitaSummaryAffiliation: Yokohama City University Country: Japan Publications
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Detail Information
Publications
The non-canonical role of Atg family members as suppressors of innate antiviral immune signalingFumihiko Takeshita
Department of Molecular Biodefense Research, Yokohama City University Graduate School of Medicine, Yokohama, Japan
Autophagy 4:67-9. 2008..In this addendum, we discuss the non-canonical role of the Atg12-Atg5 conjugate as a suppressor of innate immune responses...- Transcriptional regulation of the human TLR9 geneFumihiko Takeshita
Section of Retroviral Immunology, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, MD 20892, USA
J Immunol 173:2552-61. 2004..These findings will help to elucidate the mechanism of TLR9 gene regulation and to provide insight into the process by which TLR9 evolved in the mammalian immune system... 
Intracellular DNA sensors in immunityFumihiko Takeshita
Department of Molecular Biodefense Research, Yokohama City University Graduate School of Medicine, 3 9 Fukuura, Kanazawaku, Yokohama, Japan
Curr Opin Immunol 20:383-8. 2008....- Muscle creatine kinase/SV40 hybrid promoter for muscle-targeted long-term transgene expressionFumihiko Takeshita
Department of Molecular Biodefense Research, Yokohama City University Graduate School of Medicine, 3 9 Fukuura, Kanazawa Ku, Yokohama 236 0004, Japan
Int J Mol Med 19:309-15. 2007.... - TRAF4 acts as a silencer in TLR-mediated signaling through the association with TRAF6 and TRIFFumihiko Takeshita
Department of Molecular Biodefense Research, Yokohama City University School of Medicine, Yokohama, Japan
Eur J Immunol 35:2477-85. 2005..These results suggest that TRAF4 participates in the molecular mechanism underlying silencing of TLR-mediated signaling through the interaction with molecules harboring phagosome/endosome membrane... 
Toll-like receptor adaptor molecules enhance DNA-raised adaptive immune responses against influenza and tumors through activation of innate immunityFumihiko Takeshita
Department of Molecular Biodefense Research, Yokohama City University Graduate School of Medicine, 3 9 Fukuura, Kanazawaku, Yokohama 236 0004, Japan
J Virol 80:6218-24. 2006..These results indicate that TLR adaptor molecules can bridge innate and adaptive immunity and potentiate the effects of DNA vaccines against virus infection and tumors...- Repetitive elements in mammalian telomeres suppress bacterial DNA-induced immune activationIhsan Gursel
Section of Retroviral Immunology, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, MD 20892, USA
J Immunol 171:1393-400. 2003..These findings are the first to establish that specific host-derived molecules can down-regulate the innate immune response elicited by a TLR ligand... - Blocking of the TLR5 activation domain hampers protective potential of flagellin DNA vaccineSukumar Saha
Department of Molecular Biodefense Research, Yokohama City University Graduate School of Medicine, 3 9 Fukuura, Kanazawa Ku, Yokohama, Japan
J Immunol 179:1147-54. 2007.... - Extrachromosomal histone H2B mediates innate antiviral immune responses induced by intracellular double-stranded DNAKouji Kobiyama
Department of Molecular Biodefense Research, Yokohama City University Graduate School of Medicine, 3 9 Fukuura, Kanazawaku, Yokohama 236 0004, Japan
J Virol 84:822-32. 2010..Thus, the present findings provide evidence indicating that the extrachromosomal histone H2B is engaged in the signaling pathway initiated by dsDNA to trigger antiviral innate immune responses... - The degree of apoptosis as an immunostimulant for a DNA vaccine against HIV-1 infectionYoshitsugu Kojima
Department of Molecular Biodefense Research, Yokohama City University School of Medicine, 3 9 Fukuura, Kanazawa Ku, Yokohama 236 0004, Japan
Vaccine 25:438-45. 2007..These results suggest that the appropriate level of apoptosis is important for DNA vaccine development... - Signal transduction pathways mediated by the interaction of CpG DNA with Toll-like receptor 9Fumihiko Takeshita
Center for Biologics Evaluation and Research Food and Drug Administration, Bldg 29A, Rm 3D10, Bethesda, MD 20892, USA
Semin Immunol 16:17-22. 2004.... - The Atg5 Atg12 conjugate associates with innate antiviral immune responsesNao Jounai
Department of Molecular Biodefense Research, Yokohama City University Graduate School of Medicine, Yokohama 236 0004, Japan
Proc Natl Acad Sci U S A 104:14050-5. 2007..Thus, in contrast to its role in promoting the bactericidal process, a component of the autophagic machinery appears to block innate antiviral immune responses, thereby contributing to RNA virus replication in host cells... - A fused gene of nucleoprotein (NP) and herpes simplex virus genes (VP22) induces highly protective immunity against different subtypes of influenza virusSukumar Saha
Department of Molecular Biodefense Research, Yokohama City University Graduate School of Medicine, 3-9 Fukuura, Yokohama 236-0004, Japan
Virology 354:48-57. 2006..These results indicate that a plasmid DNA vaccine encoding pVP22 plus NP induces a high level of cross-protective immunity against influenza virus subtypes... - A signaling polypeptide derived from an innate immune adaptor molecule can be harnessed as a new class of vaccine adjuvantKouji Kobiyama
Department of Molecular Biodefense Research, Yokohama City University Graduate School of Medicine, Yokohama, Japan
J Immunol 182:1593-601. 2009.... - CpG RNA: identification of novel single-stranded RNA that stimulates human CD14+CD11c+ monocytesTakahiro Sugiyama
Section of Retroviral Immunology, Division of Viral Products, Center for Biologics, Evaluation and Research, Food and Drug Administration, Bethesda, MD 20892, USA
J Immunol 174:2273-9. 2005..These data suggest that CpG ORN represent a novel class of synthetic immunostimulatory nucleic acids with distinct target cells, receptors, and functions from that of previously known immunomodulatory nucleic acids... - Effect of suppressive DNA on CpG-induced immune activationHiroshi Yamada
Section of Retroviral Immunology, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, MD 20892, USA
J Immunol 169:5590-4. 2002..These findings contribute to our understanding of the immunomodulatory activity of DNA-based products and the rules that govern immune recognition of stimulatory and suppressive motifs... - Potential role of phosphatidylinositol 3 kinase, rather than DNA-dependent protein kinase, in CpG DNA-induced immune activationKen J Ishii
Section of Retroviral Immunology, Division of Viral Products, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, MD 20892, USA
J Exp Med 196:269-74. 2002..These results support a model in which CpG signaling is mediated through TLR-9 but not DNA-PK, and suggest that wortmannin-sensitive member(s) of the PI3-kinase family play a critical role in shuttling CpG DNA to TLR-9... - Multivalent DNA vaccine protects mice against pulmonary infection caused by Pseudomonas aeruginosaSukumar Saha
Department of Molecular Biodefense Research, Yokohama City University Graduate School of Medicine, Yokohama 236-0004, Japan
Vaccine 24:6240-9. 2006..These results implied the possible clinical application of multivalent DNA vaccine against P. aeruginosa... - Activator protein 1-mediated transcriptional regulation strategy sustains long-term expression of a xenogeneic gene product in vivo: an implication for gene therapy targeting congenital protein deficienciesShin Sasaki
Department of Molecular Biodefense Research, Yokohama City University Graduate School of Medicine, Yokohama 236-0004, Japan
Int J Mol Med 18:289-97. 2006..Our strategy may open a new avenue for a gene therapy that involves lifelong supplementation of a deficient protein that could be targeted by the host's immune system... - CpG DNA: recognition by and activation of monocytesDennis M Klinman
Retroviral Immunology Section, Center for Biologics Evaluation and Research, FDA, Bldg 29A Rm 3 D 10, Bethesda, MD 20892, USA
Microbes Infect 4:897-901. 2002..ODNs of the "K"-type interact with Toll-like receptor 9 and induce monocytes to proliferate and secrete IL-6. In contrast, "D"-type ODNs trigger monocytes to differentiate into mature dendritic cells... - NLRP4 negatively regulates autophagic processes through an association with beclin1Nao Jounai
Department of Molecular Biodefense Research, Yokohama City University Graduate School of Medicine, Yokohama 236 0004, Japan
J Immunol 186:1646-55. 2011.... - Modulation of intracellular signaling using protein-transduction technologyKouji Kobiyama
Department of Molecular Biodefense Research, Yokohama City University Graduate School of Medicine, Kanazawaku, Yokohama, Japan
Crit Rev Immunol 30:395-421. 2010..This review introduces the mechanism of action, recent applications, and future perspectives of protein-transduction technology as an alternative therapeutic in the post-genome era... - Hyperproduction of IFN-gamma by CpG oligodeoxynucleotide-induced exacerbation of atopic dermatitis-like skin lesion in some NC/Nga miceMomoko Takakura
Department of Environmental Immuno Dermatology, Yokohama City University Graduate School of Medicine, Yokohama, Japan
J Invest Dermatol 125:1156-62. 2005..These results suggest that the suppression of Th2 cytokines may not completely prevent dermatitis and that IFN-gamma may play a role in developing dermatitis in some NC/Nga mice... - Suppression of iodide uptake and thyroid hormone synthesis with stimulation of the type I interferon system by double-stranded ribonucleic acid in cultured human thyroid folliclesKazuko Yamazaki
Thyroid Disease Institute, Kanaji Hospital, Tokyo, and Department of Molecular Biodefense Research, Yokohama City University Graduate School of Medicine, Japan
Endocrinology 148:3226-35. 2007..These in vitro findings suggest that when thyrocytes are infected with certain viruses, dsRNA formed intracellularly in thyrocytes may be a cause for thyroid dysfunction, leading to development of autoimmune thyroiditis... - Intracellular inflammatory sensors for foreign invaders and substances of self-originNao Jounai
Department of Molecular Biodefense Research, Yokohama City University Graduate School of Medicine, Yokohama, Japan
Adv Exp Med Biol 738:60-78. 2012..Further studies focusing on the role of these inflammatory sensors in the development of immune disorders would highlight new avenues for the development of novel diagnostic and therapeutic applications with regard to these diseases... - Application of innate immune molecules for a new class of drugs: infection, inflammation and beyondH J Kimura
Department of Pathology, The Johns Hopkins School of Medicine, Baltimore, MD 21205, USA
Endocr Metab Immune Disord Drug Targets 11:68-75. 2011.... - Mutant influenza A virus nucleoprotein is preferentially localized in the cytoplasm and its immunization in mice shows higher immunogenicity and cross-reactivityKenji Ohba
Department of Molecular Virology, Graduate School, Tokyo Medical and Dental University, 1 5 45 Yushima, Bunkyo ku, Tokyo 113 8519, Japan
Vaccine 25:4291-300. 2007..Therefore, DNA-based vaccination with NPm may contribute to the development of protective immunity against diverse influenza virus through its ability to stimulate cellular immunity... - Interleukin-1 receptor-associated kinase-1 plays an essential role for Toll-like receptor (TLR)7- and TLR9-mediated interferon-{alpha} inductionSatoshi Uematsu
Department of Host Defense, Research Institute for Microbial Diseases, Osaka University, Suita Osaka 565 0871, Japan
J Exp Med 201:915-23. 2005..These results indicated that IRAK-1 is a specific regulator for TLR7- and TLR9-mediated IFN-alpha induction in pDCs... - Role of the polypeptide region of a 33kDa mycobacterial lipoprotein for efficient IL-12 productionYasuko Yamashita
Department of Microbiology, Leprosy Research Center, National Institute of Infectious Diseases, 4-2-1 Aobacho, Higashimurayama, Tokyo 189-0002, Japan
Cell Immunol 229:13-20. 2004..Therefore, both peptide regions of LpK and lipid residues are necessary for efficient IL-12 production... - A Toll-like receptor-independent antiviral response induced by double-stranded B-form DNAKen J Ishii
Exploratory Research for Advanced Technology, Japan Science and Technology Agency, Research Institute for Microbial Diseases, Osaka University, Osaka 565 0871, Japan
Nat Immunol 7:40-8. 2006..These results suggest that both TBK1 and IKKi are required for innate immune activation by B-DNA, which might be important in antiviral innate immunity and other DNA-associated immune disorders... - Effect of hsp65 DNA vaccination carrying immunostimulatory DNA sequences (CpG motifs) against Mycobacterium leprae multiplication in miceHiroko Nomaguchi
Leprosy Research Center, National Institute of Infectious Diseases, 4-2-1-Aobacho, Higashimurayama, Tokyo, Japan
Int J Lepr Other Mycobact Dis 70:182-90. 2002..leprae. These results suggest that a DNA containing M. leprae-derived hsp65 and immunostimulatory sequences might be a potent vaccine candidate against M. leprae infection... - Adjuvant formulations and delivery systems for DNA vaccinesShin Sasaki
Department of Bioregulation, Leprosy Research Center, National Institute of Infectious Diseases, 189-0002 Tokyo, Japan
Methods 31:243-54. 2003..We also offer a practical guide for the procedure of intramuscular DNA injection... - Improvement of DNA vaccine immunogenicity by a dual antigen expression systemShin Sasaki
Department of Bioregulation, Leprosy Research Center, National Institute of Infectious Diseases, 4 2 1 Aoba cho, Higashimurayama, Tokyo 189 0002, Japan
Biochem Biophys Res Commun 315:38-43. 2004..We concluded that increasing the number of antigen expression cassettes in a vaccine construct improved antigen expression in the transduced tissue, which induced stronger DNA-raised immune responses... - Comparison of antigen-specific cellular immune responses between individual PBMCs and splenocytesKe-Qin Xin
Vaccine 23:2954-5. 2005 - Molecular and cellular mechanisms of DNA vaccinesCevayir Coban
Laboratory of Host Defense, Immunology Frontier Research Center, World Premier Institute for Immunology, Suita, Osaka, Japan
Hum Vaccin 4:453-6. 2008..This additional information about the mechanism of action of DNA vaccines will lead to improvements in their efficacy and safety... - [Molecular mechanisms for intracellular parasitisation and exclusion in macrophage infected with Mycobacterium leprae]Kazunari Tanigawa
Department of Bioregulation, Leprosy Research Center, National Institute of Infectious Diseases, Higashimurayama, Tokyo, Japan
Nihon Hansenbyo Gakkai Zasshi 77:57-61. 2008..However, the functional relationship between TLR2 and CORO1A was not known. We show here that there is a functional counteraction between TLR2 and CORO1A... - TLR9 expression and function is abolished by the cervical cancer-associated human papillomavirus type 16Uzma A Hasan
International Agency for Research on Cancer World Health Organization, 150 cours Albert Thomas, 69372 Lyon Cedex 08, France
J Immunol 178:3186-97. 2007.... - Guidelines for the use and interpretation of assays for monitoring autophagy in higher eukaryotesDaniel J Klionsky
Life Sciences Institute, University of Michigan, Ann Arbor, Michigan 48109 2216, USA
Autophagy 4:151-75. 2008..In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to verify an autophagic response... - Combination of DNA vaccine and adenovirus vector by cutaneous administration induced strong HIV-specific cellular immune responses in miceMomoko Takakura
Vaccine 23:847-8. 2005 - TANK-binding kinase-1 delineates innate and adaptive immune responses to DNA vaccinesKen J Ishii
Exploratory Research for Advanced Technology ERATO, Japan Science and Technology Agency JST
Nature 451:725-9. 2008..These data suggest that TBK1 is a key signalling molecule for DNA-vaccine-induced immunogenicity, by differentially controlling DNA-activated innate immune signalling through haematopoietic and non-haematopoietic cells...