Shigeo Ohno

Summary

Affiliation: Yokohama City University
Country: Japan

Publications

  1. pmc An essential role of the universal polarity protein, aPKClambda, on the maintenance of podocyte slit diaphragms
    Tomonori Hirose
    Department of Molecular Biology, Yokohama City University Graduate School of Medical Science, Yokohama, Kanagawa, Japan
    PLoS ONE 4:e4194. 2009
  2. pmc N- and C-terminal Upf1 phosphorylations create binding platforms for SMG-6 and SMG-5:SMG-7 during NMD
    Yukiko Okada-Katsuhata
    Department of Molecular Biology, Yokohama City University, School of Medicine, 3 9, Fuku ura, Kanazawa Ku, Yokohama 236 0004, Japan
    Nucleic Acids Res 40:1251-66. 2012
  3. pmc Atypical protein kinase C is involved in the evolutionarily conserved par protein complex and plays a critical role in establishing epithelia-specific junctional structures
    A Suzuki
    Department of Molecular Biology, Yokohama City University School of Medicine, Yokohama 236 0004, Japan
    J Cell Biol 152:1183-96. 2001
  4. pmc A cell polarity protein aPKClambda is required for eye lens formation and growth
    Yuki Sugiyama
    School of Biological and Biomedical Sciences, University of Durham, Durham, UK
    Dev Biol 336:246-56. 2009
  5. ncbi request reprint Intercellular junctions and cellular polarity: the PAR-aPKC complex, a conserved core cassette playing fundamental roles in cell polarity
    S Ohno
    Department of Molecular Biology, Yokohama City University School of Medicine, 3 9, Fuku ura, Kanazawa Ku, Yokohama 236 0004, Japan
    Curr Opin Cell Biol 13:641-8. 2001
  6. ncbi request reprint Protein kinase C isotypes and their specific functions: prologue
    Shigeo Ohno
    Department of Molecular Biology, Yokohama City University School of Medicine, Yokohama 236 0004, Japan
    J Biochem 132:509-11. 2002
  7. pmc SMG-8 and SMG-9, two novel subunits of the SMG-1 complex, regulate remodeling of the mRNA surveillance complex during nonsense-mediated mRNA decay
    Akio Yamashita
    Department of Molecular Biology, Yokohama City University School of Medicine, Yokohama, Japan
    Genes Dev 23:1091-105. 2009
  8. doi request reprint Interaction between PAR-3 and the aPKC-PAR-6 complex is indispensable for apical domain development of epithelial cells
    Yosuke Horikoshi
    Department of Molecular Biology, Yokohama City University Graduate School of Medical Science, 3 9 Fuku ura, Kanazawa Ku, Yokohama 236 0004, Japan
    J Cell Sci 122:1595-606. 2009
  9. ncbi request reprint Inactivation of aPKClambda results in the loss of adherens junctions in neuroepithelial cells without affecting neurogenesis in mouse neocortex
    Fumiyasu Imai
    Department of Molecular Biology, Yokohama City University Graduate School of Medical Science, 3 9 Fuku ura, Kanazawa Ku, Yokohama 236 0004, Japan
    Development 133:1735-44. 2006
  10. ncbi request reprint The c-Jun N-terminal kinase activator dual leucine zipper kinase regulates axon growth and neuronal migration in the developing cerebral cortex
    Syu ichi Hirai
    Department of Molecular Biology, Graduate School of Medical Science, Yokohama City University, Yokohama 236 0004, Japan
    J Neurosci 26:11992-2002. 2006

Collaborators

Detail Information

Publications148 found, 100 shown here

  1. pmc An essential role of the universal polarity protein, aPKClambda, on the maintenance of podocyte slit diaphragms
    Tomonori Hirose
    Department of Molecular Biology, Yokohama City University Graduate School of Medical Science, Yokohama, Kanagawa, Japan
    PLoS ONE 4:e4194. 2009
    ..These observations not only establish a critical function of the polarity proteins in the maintenance of slit diaphragms, but also imply their potential involvement in renal failure in FSGS...
  2. pmc N- and C-terminal Upf1 phosphorylations create binding platforms for SMG-6 and SMG-5:SMG-7 during NMD
    Yukiko Okada-Katsuhata
    Department of Molecular Biology, Yokohama City University, School of Medicine, 3 9, Fuku ura, Kanazawa Ku, Yokohama 236 0004, Japan
    Nucleic Acids Res 40:1251-66. 2012
    ....
  3. pmc Atypical protein kinase C is involved in the evolutionarily conserved par protein complex and plays a critical role in establishing epithelia-specific junctional structures
    A Suzuki
    Department of Molecular Biology, Yokohama City University School of Medicine, Yokohama 236 0004, Japan
    J Cell Biol 152:1183-96. 2001
    ..These results indicate that aPKC is involved in the evolutionarily conserved PAR protein complex, and plays critical roles in the development of the junctional structures and apico-basal polarization of mammalian epithelial cells...
  4. pmc A cell polarity protein aPKClambda is required for eye lens formation and growth
    Yuki Sugiyama
    School of Biological and Biomedical Sciences, University of Durham, Durham, UK
    Dev Biol 336:246-56. 2009
    ..These observations indicate that aPKClambda, a regulator of polarity and apical junctions, is required for development of a lens that is the correct size and shape...
  5. ncbi request reprint Intercellular junctions and cellular polarity: the PAR-aPKC complex, a conserved core cassette playing fundamental roles in cell polarity
    S Ohno
    Department of Molecular Biology, Yokohama City University School of Medicine, 3 9, Fuku ura, Kanazawa Ku, Yokohama 236 0004, Japan
    Curr Opin Cell Biol 13:641-8. 2001
    ..Recent studies on the role of this PAR-aPKC complex in epithelial cell polarization provide new insights into the molecular basis of epithelial junctional formation and cell polarity...
  6. ncbi request reprint Protein kinase C isotypes and their specific functions: prologue
    Shigeo Ohno
    Department of Molecular Biology, Yokohama City University School of Medicine, Yokohama 236 0004, Japan
    J Biochem 132:509-11. 2002
  7. pmc SMG-8 and SMG-9, two novel subunits of the SMG-1 complex, regulate remodeling of the mRNA surveillance complex during nonsense-mediated mRNA decay
    Akio Yamashita
    Department of Molecular Biology, Yokohama City University School of Medicine, Yokohama, Japan
    Genes Dev 23:1091-105. 2009
    ....
  8. doi request reprint Interaction between PAR-3 and the aPKC-PAR-6 complex is indispensable for apical domain development of epithelial cells
    Yosuke Horikoshi
    Department of Molecular Biology, Yokohama City University Graduate School of Medical Science, 3 9 Fuku ura, Kanazawa Ku, Yokohama 236 0004, Japan
    J Cell Sci 122:1595-606. 2009
    ..Therefore, formation of the PAR-3-aPKC-PAR-6 complex might be required for targeting of not only the aPKC-PAR-6 complex but also of apical protein carrier vesicles to primordial junction structures...
  9. ncbi request reprint Inactivation of aPKClambda results in the loss of adherens junctions in neuroepithelial cells without affecting neurogenesis in mouse neocortex
    Fumiyasu Imai
    Department of Molecular Biology, Yokohama City University Graduate School of Medical Science, 3 9 Fuku ura, Kanazawa Ku, Yokohama 236 0004, Japan
    Development 133:1735-44. 2006
    ..Therefore, we concluded that, at least in the later stages of neurogenesis, regulation of cell cycle exit is independent of adherens junctions...
  10. ncbi request reprint The c-Jun N-terminal kinase activator dual leucine zipper kinase regulates axon growth and neuronal migration in the developing cerebral cortex
    Syu ichi Hirai
    Department of Molecular Biology, Graduate School of Medical Science, Yokohama City University, Yokohama 236 0004, Japan
    J Neurosci 26:11992-2002. 2006
    ..These data suggest that DLK plays a significant role in the coordinated regulation of radial migration and axon projection by modulating JNK activity...
  11. doi request reprint Axon formation in neocortical neurons depends on stage-specific regulation of microtubule stability by the dual leucine zipper kinase-c-Jun N-terminal kinase pathway
    Syu ichi Hirai
    Department of Molecular Biology, Yokohama City University Graduate School of Medicine, Yokohama 236 0004, Japan
    J Neurosci 31:6468-80. 2011
    ..Together, these in vivo and in vitro observations indicate that the DLK-JNK pathway facilitates axon formation in neocortical neurons via stage-specific regulation of microtubule stability...
  12. pmc Affixin interacts with alpha-actinin and mediates integrin signaling for reorganization of F-actin induced by initial cell-substrate interaction
    Satoshi Yamaji
    The First Dept of Internal Medicine, Yokohama City University School of Medicine, 3 9 Fuku ura, Kanazawa Ku, Yokohama 236 0004, Japan
    J Cell Biol 165:539-51. 2004
    ....
  13. ncbi request reprint aPKC acts upstream of PAR-1b in both the establishment and maintenance of mammalian epithelial polarity
    Atsushi Suzuki
    Department of Molecular Biology, Yokohama City University School of Medicine, Yokohama 236 0004, Japan
    Curr Biol 14:1425-35. 2004
    ....
  14. ncbi request reprint Self-association of PAR-3-mediated by the conserved N-terminal domain contributes to the development of epithelial tight junctions
    Keiko Mizuno
    Department of Molecular Biology, Yokohama City University School of Medicine, Fuku ura 3 9, Kanazawa Ku, Yokohama 236 0004, Japan
    J Biol Chem 278:31240-50. 2003
    ..These results support the notion that the CR1-mediated self-association of the PAR-3-containing protein complex plays a role during the formation of functional tight junctions...
  15. pmc Binding of a novel SMG-1-Upf1-eRF1-eRF3 complex (SURF) to the exon junction complex triggers Upf1 phosphorylation and nonsense-mediated mRNA decay
    Isao Kashima
    Department of Molecular Biology, Yokohama City University School of Medicine, Yokohama 236 0004, Japan
    Genes Dev 20:355-67. 2006
    ..Thus, the SMG-1-mediated phosphorylation of Upf1 occurs on the association of SURF with EJC, which provides the link between the EJC and recognition of PTCs and triggers NMD...
  16. ncbi request reprint PAR3 is essential for cyst-mediated epicardial development by establishing apical cortical domains
    Tomonori Hirose
    Department of Molecular Biology, Yokohama City University Graduate School of Medical Science, Yokohama 236 0004, Japan
    Development 133:1389-98. 2006
    ..These results also provide a clear example of the proper organization of epithelial tissues through the regulation of individual cell polarity...
  17. doi request reprint High expression of KIBRA in low atypical protein kinase C-expressing gastric cancer correlates with lymphatic invasion and poor prognosis
    Yohei Yoshihama
    Department of Molecular Biology, Graduate School of Medical Science, Yokohama City University, Yokohama, Japan
    Cancer Sci 104:259-65. 2013
    ..These results suggest that high expression of KIBRA in low aPKC-expressing cells causes massive loss of aPKC activity, leading to loss of polarity and invasiveness of gastric cancer cells...
  18. ncbi request reprint Lgl mediates apical domain disassembly by suppressing the PAR-3-aPKC-PAR-6 complex to orient apical membrane polarity
    Tomoyuki Yamanaka
    Department of Molecular Biology, Yokohama City University, Graduate School of Medical Science, 3 9 Fuku ura, Kanazawa Ku, Yokohama 236 0004, Japan
    J Cell Sci 119:2107-18. 2006
    ....
  19. doi request reprint AAA+ proteins RUVBL1 and RUVBL2 coordinate PIKK activity and function in nonsense-mediated mRNA decay
    Natsuko Izumi
    Department of Molecular Biology, Yokohama City University School of Medicine, Yokohama, Japan
    Sci Signal 3:ra27. 2010
    ..Thus, RUVBL1 and RUVBL2 regulate PIKK functions on two different levels: They control the abundance of PIKKs, and they stimulate the formation of PIKK-containing molecular complexes, such as those involved in NMD...
  20. ncbi request reprint [Regulation of cell polarity in establishment of epithelium]
    Fumiyasu Imai
    Department of Molecular Biology, Yokohama City University School of Medicine, 3 9 Fukuura, Kanazawa Ku, Yokohama 236 0004, Japan
    Seikagaku 78:622-30. 2006
  21. ncbi request reprint Mammalian Lgl forms a protein complex with PAR-6 and aPKC independently of PAR-3 to regulate epithelial cell polarity
    Tomoyuki Yamanaka
    Department of Molecular Biology, Yokohama City University School of Medicine, 3 9 Fuku ura, Kanazawa Ku, Japan
    Curr Biol 13:734-43. 2003
    ..However, how physically separated proteins interact remains to be clarified...
  22. pmc aPKClambda/iota promotes growth of prostate cancer cells in an autocrine manner through transcriptional activation of interleukin-6
    Hitoshi Ishiguro
    Department of Urology, Yokohama City University Graduate School of Medicine, 3 9 Fukuura, Kanazawa Ku, Yokohama, Kanagawa 236 0004, Japan
    Proc Natl Acad Sci U S A 106:16369-74. 2009
    ..Further, aPKClambda/iota expression might be a biomarker for prostate cancer progression...
  23. doi request reprint The 8th and 9th tandem spectrin-like repeats of utrophin cooperatively form a functional unit to interact with polarity-regulating kinase PAR-1b
    Kazunari Yamashita
    Department of Molecular Biology, Yokohama City University Graduate School of Medical Science, 3 9 Fuku ura, Kanazawa Ku, Yokohama 236 0004, Japan
    Biochem Biophys Res Commun 391:812-7. 2010
    ..These results reveal a novel function of the rod domain of utrophin beyond that of a passive structural linker connecting the N- and C-terminal domain...
  24. ncbi request reprint Association of ASIP/mPAR-3 with adherens junctions of mouse neuroepithelial cells
    Naoyuki Manabe
    Department of Molecular Biology, Yokohama City University School of Medicine, Kanazawa Ku, Yokohama, Japan
    Dev Dyn 225:61-9. 2002
    ....
  25. ncbi request reprint aPKC kinase activity is required for the asymmetric differentiation of the premature junctional complex during epithelial cell polarization
    Atsushi Suzuki
    Department of Molecular Biology, Yokohama City University School of Medicine, 3 9 Fuku ura, Kanazawa Ku, Yokohama 236 0004, Japan
    J Cell Sci 115:3565-73. 2002
    ..These results suggest that aPKC contributes to the establishment of epithelial cell polarity by promoting the transition of fibroblastic junctional structures into epithelia-specific asymmetric ones...
  26. doi request reprint aPKC enables development of zonula adherens by antagonizing centripetal contraction of the circumferential actomyosin cables
    Masaru Kishikawa
    Department of Molecular Biology, Yokohama City University Graduate School of Medical Science, 3 9 Fuku ura, Kanazawa Ku, Yokohama, Japan
    J Cell Sci 121:2481-92. 2008
    ..The present results provide novel insights into not only the site of action of aPKC kinase activity but also the role of actomyosin contraction in epithelial polarization...
  27. ncbi request reprint The gamma-parvin-integrin-linked kinase complex is critically involved in leukocyte-substrate interaction
    Ryusuke Yoshimi
    Department of Internal Medicine and Clinical Immunology, Yokohama City Graduate School of Medicine, 3 9 Fuku ura, Kanazawa Ku, Yokohama 236 0004, Japan
    J Immunol 176:3611-24. 2006
    ....
  28. ncbi request reprint Involvement of ASIP/PAR-3 in the promotion of epithelial tight junction formation
    Tomonori Hirose
    Department of Molecular Biology, Yokohama City University School of Medicine, Kanazawa Ku, Japan
    J Cell Sci 115:2485-95. 2002
    ..Together, our present data suggest that ASIP/PAR-3 regulates epithelial tight junction formation positively through interaction with aPKC...
  29. doi request reprint Coexpression of aPKCλ/ι and IL-6 in prostate cancer tissue correlates with biochemical recurrence
    Hitoshi Ishiguro
    Department of Urology, Yokohama City University Graduate School of Medicine, Kanagawa, Japan
    Cancer Sci 102:1576-81. 2011
    ..03 in the multivariate analysis). These results indicate a strong correlation between aPKCλ/ι and IL-6 in prostate tumors, and that the aPKCλ/ι-IL-6 axis is a reliable prognostic factor for the biochemical recurrence of this cancer...
  30. doi request reprint ASPP2 regulates epithelial cell polarity through the PAR complex
    Weili Cong
    Department of Molecular Biology, Yokohama City University Graduate School of Medical Science, 3 9 Fuku ura, Kanazawa Ku, Yokohama, Japan
    Curr Biol 20:1408-14. 2010
    ..Our results, taken together with the known functions of ASPP2, suggest a close relationship between cell polarity and other cell regulatory mechanisms mediated by ASPP2...
  31. ncbi request reprint Developmental changes in the expression pattern of the JNK activator kinase MUK/DLK/ZPK and active JNK in the mouse cerebellum
    Jun Suenaga
    Department of Molecular Biology, Yokohama City University Graduate School of Medical Science, 3 9 Fukuura, Kanazawa Ku, Yokohama 236 0004, Japan
    Cell Tissue Res 325:189-95. 2006
    ....
  32. ncbi request reprint The PAR-aPKC system: lessons in polarity
    Atsushi Suzuki
    Department of Molecular Biology, Yokohama City University Graduate School of Medical Science, 3 9 Fuku ura, Kanazawa Ku, Yokohama 236 0004, Japan
    J Cell Sci 119:979-87. 2006
    ....
  33. ncbi request reprint sPAR-3, a splicing variant of PAR-3, shows cellular localization and an expression pattern different from that of PAR-3 during enterocyte polarization
    Takako Yoshii
    The Third Department of Internal Medicine, Yokohama City University School of Medicine, Fuku ura 3 9, Kanazawa Ku, Yokohama 236 0004, Japan
    Am J Physiol Gastrointest Liver Physiol 288:G564-70. 2005
    ..This makes a contrast to PAR-3, which concentrates at tight junctions in fully polarized cells. These results provide evidence suggesting the difference in the role between sPAR-3 and PAR-3 in epithelial cells...
  34. ncbi request reprint Muscle develops a specific form of small heat shock protein complex composed of MKBP/HSPB2 and HSPB3 during myogenic differentiation
    Y Sugiyama
    Department of Molecular Biology, Yokohama City University School of Medicine, Kanazawa Ku, Yokohama 236 0004, Japan
    J Biol Chem 275:1095-104. 2000
    ..Taken together, the results suggest that muscle cells develop two kinds of stress response systems composed of diverged sHSP members, and that these systems work independently in muscle maintenance and differentiation...
  35. ncbi request reprint Activation of the JNK pathway by distantly related protein kinases, MEKK and MUK
    S Hirai
    Department of Molecular Biology, Yokohama City University School of Medicine, Japan
    Oncogene 12:641-50. 1996
    ..The structural divergence between MUK and MEKK reveals the multiplicity of signaling pathways that activate JNK/SAPKs...
  36. ncbi request reprint Possible role of ILK-affixin complex in integrin-cytoskeleton linkage during platelet aggregation
    Satoshi Yamaji
    The First Department of Internal Medicine, Yokohama City University School of Medicine, 3 9 Fuku ura, Kanazawa Ku, 236 0004, Yokohama, Japan
    Biochem Biophys Res Commun 297:1324-31. 2002
    ....
  37. ncbi request reprint Regulated protein-protein interaction between aPKC and PAR-3 plays an essential role in the polarization of epithelial cells
    Yoko Nagai-Tamai
    Department of Molecular Biology, Yokohama City University School of Medicine, Yokohama 236 0004, Japan
    Genes Cells 7:1161-71. 2002
    ..Here, we investigated the biochemical nature of the aPKC-PAR-3 interaction in detail to clarify its functional importance in cell polarity...
  38. ncbi request reprint Phosphorylation of hUPF1 induces formation of mRNA surveillance complexes containing hSMG-5 and hSMG-7
    Tetsuo Ohnishi
    Department of Molecular Biology, Yokohama City University School of Medicine, Yokohama 236 0004, Japan
    Mol Cell 12:1187-200. 2003
    ..We propose that sequential phosphorylation and dephosphorylation of hUPF1 by hSMG-1 and PP2A, respectively, contribute to the remodeling of the mRNA surveillance complex...
  39. doi request reprint High expression of atypical protein kinase C lambda/iota in gastric cancer as a prognostic factor for recurrence
    Ryo Takagawa
    Department of Gastroenterological Surgery, Yokohama City University Graduate School of Medicine, Yokohama, Japan
    Ann Surg Oncol 17:81-8. 2010
    ..This study aimed to clarify the relationship between aPKClambda/iota expression and the clinicopathological features of gastric cancer...
  40. ncbi request reprint Expression of MUK/DLK/ZPK, an activator of the JNK pathway, in the nervous systems of the developing mouse embryo
    Syu ichi Hirai
    Department of Molecular Biology, Yokohama City University Graduate School of Medical Science, 3 9, Fukuura, Kanazawa Ku, Yokohama 236 0004, Japan
    Gene Expr Patterns 5:517-23. 2005
    ..The intensely overlapping localization of betaIII-tubulin and MUK/DLK/ZPK indicated that this protein kinase is tightly associated with the microtubules of neurons...
  41. pmc The interaction of Kinesin-1 with its adaptor protein JIP1 can be regulated via proteins binding to the JIP1-PTB domain
    Tomoko Satake
    Department of Molecular Biology, Yokohama City University Graduate School of Medicine, Yokohama 236 0004, Japan
    BMC Cell Biol 14:12. 2013
    ..The kinesin-1-binding protein, JIP1, can function as an adaptor protein that links kinesin-1 and other JIP1-binding "cargo" proteins. However, it is unknown whether these "cargo" proteins influence the JIP1-kinesin-1 binding...
  42. doi request reprint Intracellular polarity protein PAR-1 regulates extracellular laminin assembly by regulating the dystroglycan complex
    Maki Masuda-Hirata
    Department of Molecular Biology, Yokohama City University Graduate School of Medical Science, Kanazawa Ku, Yokohama, Japan
    Genes Cells 14:835-50. 2009
    ..These results reveal the presence of a novel inside-out pathway in which an intracellular polarity protein regulates the ECM organization required for epithelial cell polarity and tissue morphogenesis...
  43. pmc An atypical PKC directly associates and colocalizes at the epithelial tight junction with ASIP, a mammalian homologue of Caenorhabditis elegans polarity protein PAR-3
    Y Izumi
    Department of Molecular Biology, Yokohama City University School of Medicine, Yokohama 236 0004, Japan
    J Cell Biol 143:95-106. 1998
    ....
  44. ncbi request reprint Protein kinase C lambda/iota (PKClambda/iota): a PKC isotype essential for the development of multicellular organisms
    Atsushi Suzuki
    Department of Molecular Biology, Yokohama City University School of Medicine, 3 9 Fuku ura, Kanazawa Ku, Yokohama 239 0004
    J Biochem 133:9-16. 2003
    ....
  45. ncbi request reprint PAR-6 regulates aPKC activity in a novel way and mediates cell-cell contact-induced formation of the epithelial junctional complex
    T Yamanaka
    Department of Molecular Biology, Yokohama City University School of Medicine, 3-9 Fuku-ura, Kanazawa-ku, Yokohama 236-0004, Japan
    Genes Cells 6:721-31. 2001
    ....
  46. doi request reprint Expression and localisation of apical junctional complex proteins in lens epithelial cells
    Yuki Sugiyama
    School of Biological and Biomedical Sciences, Durham University, Science Park, Durham, UK
    Exp Eye Res 87:64-70. 2008
    ..Likewise, immunogold labelling for E-cadherin identified AJs within these AJCs. We also report aPKC immunogold labelling localised to the AJC. These data show that the AJC of lens epithelial cells are a composite of TJs and AJs...
  47. ncbi request reprint MAPK-upstream protein kinase (MUK) regulates the radial migration of immature neurons in telencephalon of mouse embryo
    Syu ichi Hirai
    Department of Molecular Biology, Yokohama City University School of Medicine, 3 9 Fukuura, Kanazawa Ku, Yokohama 236 0004, Japan
    Development 129:4483-95. 2002
    ..In COS-1 cells, MUK/DLK/ZPK overexpression impairs the radial organization of microtubules without massive depolymerization. These results suggest that MUK/DLK/ZPK and JNK regulate radial cell migration via microtubule-based events...
  48. doi request reprint Heat shock protein 90 regulates phosphatidylinositol 3-kinase-related protein kinase family proteins together with the RUVBL1/2 and Tel2-containing co-factor complex
    Natsuko Izumi
    Department of Molecular Biology, School of Medicine, Yokohama City University, Yokohama, Japan
    Cancer Sci 103:50-7. 2012
    ....
  49. doi request reprint Symmetrically dividing cell specific division axes alteration observed in proteasome depleted C. elegans embryo
    Yuki Sugiyama
    Department of Molecular Cell Biology, Yokohama City University Graduate School of Medical Science, 3 9 Fuku ura, Kanazawa Ku, Yokohama 236 0004, Japan
    Mech Dev 125:743-55. 2008
    ..Our data suggest that the expression of RPN-2 is involved in the mechanism to suppress nuclear-centrosome rotation in symmetrically dividing cells and it may work in cooperation with PKC-3...
  50. ncbi request reprint Neonatal pancreatic cells redifferentiate into both neural and pancreatic lineages
    Wenping Zhao
    Department of Regenerative Medicine, Graduate School of Medical Science, Yokohama City University, Yokohama 236 0004, Japan
    Biochem Biophys Res Commun 352:84-90. 2007
    ..The neuronal differentiation of pancreatic cells, however, occurs prior to endocrine differentiation and gradually becomes dominant, thus implying a default neuronal lineage specification for cultured pancreatic cells...
  51. ncbi request reprint The role of SMG-1 in nonsense-mediated mRNA decay
    Akio Yamashita
    Department of Molecular Biology, Yokohama City University School of Medicine and Graduate School of Medical Science, Kanazawa Ku, Yokohama 236 0004, Japan
    Biochim Biophys Acta 1754:305-15. 2005
    ..At present, a variety of tools are available that can specifically suppress NMD, and it is possible to examine the contribution of NMD in a variety of physiological and pathological conditions...
  52. doi request reprint Maintenance of dendritic spine morphology by partitioning-defective 1b through regulation of microtubule growth
    Kenji Hayashi
    Department of Molecular Biology, Yokohama City University Graduate School of Medical Science, Kanazawa Ku, Yokohama 236 0004, Japan
    J Neurosci 31:12094-103. 2011
    ..In conclusion, the present results suggested a novel function for PAR1b in the maintenance of mature dendritic spine morphology by regulating microtubule growth and the accumulation of p140Cap in dendritic spines...
  53. doi request reprint The overexpression and altered localization of the atypical protein kinase C lambda/iota in breast cancer correlates with the pathologic type of these tumors
    Yasuyuki Kojima
    Department of Gastroenterological Surgery, Yokohama City University Graduate School of Medicine, Yokohama 236 0004, Japan
    Hum Pathol 39:824-31. 2008
    ..This is also the first evidence to show aPKC lambda/iota overexpression in breast cancer and demonstrates that its localization is associated with the trend of pathologic type of the tumor...
  54. ncbi request reprint YSK1, a novel mammalian protein kinase structurally related to Ste20 and SPS1, but is not involved in the known MAPK pathways
    S Osada
    Department of Molecular Biology, Yokohama City University School of Medicine, Kanazawa Ku, Yokohama, Japan
    Oncogene 14:2047-57. 1997
    ..These results suggest that YSK1 may be involved in the regulation of a novel intracellular signaling pathway...
  55. pmc Genome typing of adenovirus type 34 isolated in two cases of conjunctivitis in Sapporo, Japan
    W Saitoh-Inagawa
    Department of Ophthalmology, Yokohama City University School of Medicine, Kanazawa Ku, Yokohama, Kanagawa 236 0004, Japan
    J Clin Microbiol 39:4187-9. 2001
    ..The genome types of these two isolates were identical to that of Ad34a...
  56. pmc Human SMG-1, a novel phosphatidylinositol 3-kinase-related protein kinase, associates with components of the mRNA surveillance complex and is involved in the regulation of nonsense-mediated mRNA decay
    A Yamashita
    Department of Molecular Biology, Yokohama City University School of Medicine, Yokohama 236 0004, Japan
    Genes Dev 15:2215-28. 2001
    ..Taken together, we conclude that hSMG-1 plays a critical role in NMD through the direct phosphorylation of hUPF1/SMG-2 in the evolutionally conserved mRNA surveillance complex...
  57. ncbi request reprint Structure and properties of a ubiquitously expressed protein kinase C, nPKC delta
    K Mizuno
    Department of Molecular Biology, Tokyo Metropolitan Institute of Medical Science, Japan
    Eur J Biochem 202:931-40. 1991
    ..These results suggest that nPKC delta is involved in fundamental cellular functions regulated by diacylglycerols and mimicked by phorbol esters...
  58. ncbi request reprint A new member of the third class in the protein kinase C family, PKC lambda, expressed dominantly in an undifferentiated mouse embryonal carcinoma cell line and also in many tissues and cells
    K Akimoto
    Department of Molecular Biology, Yokohama City University School of Medicine, Japan
    J Biol Chem 269:12677-83. 1994
    ..These results, in conjunction with the results obtained in parallel experiments with PKC zeta and other PKC members, suggest a biochemical similarity between PKC lambda and zeta and their clear difference from other PKC members...
  59. ncbi request reprint MAPK upstream kinase (MUK)-binding inhibitory protein, a negative regulator of MUK/dual leucine zipper-bearing kinase/leucine zipper protein kinase
    K Fukuyama
    Departments of Dermatology, 1st Internal Medicine, Molecular Biology, and Urology, Yokohama City University School of Medicine, 3 9, Fukuura, Kanazawa Ku, Yokohama 236 0004, Japan
    J Biol Chem 275:21247-54. 2000
    ..Taken together, these observations indicate that MBIP can function as a regulator of MUK/DLK/ZPK, a finding that may provide a clue to understanding the molecular mechanism of JNK/SAPK activation by hyperosmotic stress...
  60. ncbi request reprint The second phase activation of protein kinase C delta at late G1 is required for DNA synthesis in serum-induced cell cycle progression
    Koichi Kitamura
    Department of Molecular Biology, Yokohama City University School of Medicine, Fuku ura 3 9, Kanazawa Ku, Yokohama 236 0004, Japan
    Genes Cells 8:311-24. 2003
    ..However, the mode of PKC delta activation, the cell cycle stage requiring PKC delta activity, and the exact role of PKC delta at that stage remains unknown...
  61. ncbi request reprint Over-expression of PAR-3 suppresses contact-mediated inhibition of cell migration in MDCK cells
    Aki Mishima
    Department of Molecular Biology, Yokohama City University School of Medicine, 3 9 Fuku ura, Kanazawa Ku, Yokohama 236 0004, Japan
    Genes Cells 7:581-96. 2002
    ..In mammalian epithelial cells, it forms a ternary complex with aPKC and PAR-6, and is localized to the tight junction that has been suggested as being important for creating cell polarity...
  62. doi request reprint aPKC restricts the basolateral determinant PtdIns(3,4,5)P3 to the basal region
    Shoukichi Takahama
    Department of Molecular Biology, Yokohama City University Graduate School of Medical Science, 3 9 Fukuura, Kanazawa Ku, Yokohama, Kanagawa 236 0004, Japan
    Biochem Biophys Res Commun 368:249-55. 2008
    ..In support of this, overexpression of aPKClambda kn during polarization, but not after polarization, caused whole membrane distribution of PIP3 as well as defects in epithelial polarization...
  63. pmc A novel integrin-linked kinase-binding protein, affixin, is involved in the early stage of cell-substrate interaction
    S Yamaji
    The First Department of Internal Medicine, Yokohama City University School of Medicine, Yokohama 236 0004, Japan
    J Cell Biol 153:1251-64. 2001
    ..The coexpression of ILK enhances this effect. These results provide evidence suggesting that affixin is involved in integrin-ILK signaling required for the establishment of cell-substrate adhesion...
  64. ncbi request reprint New genome type of adenovirus serotype 19 causing nosocomial infections of epidemic keratoconjunctivitis in Japan
    K Tanaka-Yokogui
    Department of Ophthalmology, Yokohama City University School of Medicine, 3 9 Fukuura, Kanazawa Ku, Yokohama 236 0004, Japan
    J Med Virol 65:530-3. 2001
    ..Strains from 1992 and 1993 showed restriction patterns similar to those of the worldwide reported variant 19a for all enzymes used. The changes detected in strains from 1997 and 1998 could be the reason for the recent epidemic...
  65. ncbi request reprint Cloning and characterization of the murine PKC alpha promoter: identification of a retinoic acid response element
    D S Desai
    Department of Molecular Biology, Yokohama City University School of Medicine, 3 9 Fuku ura, Kanazawa Ku, Yokohama, 236, Japan
    Biochem Biophys Res Commun 263:28-34. 1999
    ..These results suggest that retinoic acid increases PKC alpha gene expression in B16 cells, at least in part, through direct transcriptional stimulation of its promoter...
  66. doi request reprint Conditionally replicative adenoviral vectors for imaging the effect of chemotherapy on pancreatic cancer cells
    Jun Kimura
    Department of Gastroenterological Surgery, Graduate School of Medicine, Yokohama City University, Yokohama, Japan
    Cancer Sci 104:1083-90. 2013
    ..96), but not with gross tumor volume (R = 0.31). Therefore, CRAds might be reliable imaging tools for monitoring chemotherapy in pancreatic cancer, and could improve our ability to distinguish viable tumor cells from scar tissue...
  67. ncbi request reprint Loss of von Hippel-Lindau protein causes cell density dependent deregulation of CyclinD1 expression through hypoxia-inducible factor
    Masaya Baba
    Department of Molecular Biology, Yokohama City University School of Medicine, 3 9, Fuko ura, Kanazawaka ku, Yokohama 236 0004, Japan
    Oncogene 22:2728-38. 2003
    ..Taken together, these observations indicate that VHL is required for the downregulation of cyclinD1 at a high cell density through HIF...
  68. pmc Integrated regulation of PIKK-mediated stress responses by AAA+ proteins RUVBL1 and RUVBL2
    Natsuko Izumi
    Department of Molecular Biology, Yokohama City University School of Medicine, Yokohama, Japan
    Nucleus 3:29-43. 2012
    ..We also discuss a putative "PIKK regulatory chaperone complex" including other PIKK regulators, Hsp90 and the Tel2 complex...
  69. ncbi request reprint PAR-1/MARK: a kinase essential for maintaining the dynamic state of microtubules
    Kenji Hayashi
    Department of Molecular Biology, Yokohama City University Graduate School of Medical Science, Yokohama, Japan
    Cell Struct Funct 37:21-5. 2012
    ..Kurihara, Y., Hoogenraad, C.C., and Ohno, S. (2011). J. Neurosci., 31: 12094-12103). Importantly, our results further revealed the novel physiological function of PAR-1b in maintaining dendritic spine morphology in mature neurons...
  70. ncbi request reprint Differential induction of protein kinase C isoforms at the cardiac hypertrophy stage and congestive heart failure stage in Dahl salt-sensitive rats
    Yuichi Koide
    Department of Medicine II, Yokohama City University School of Medicine, Yokohama, Japan
    Hypertens Res 26:421-6. 2003
    ....
  71. ncbi request reprint [Regulation of epithelial cell polarity mediated by aPKC-PAR complex]
    Keiko Mizuno
    Tanpakushitsu Kakusan Koso 51:782-8. 2006
  72. ncbi request reprint [mRNA surveillance]
    Akio Yamashita
    Tanpakushitsu Kakusan Koso 47:101-12. 2002
  73. ncbi request reprint Diabetes alters neurite regeneration from mouse retinal explants in culture
    M Takano
    Department of Ophthalmology, Yokohama City University School of Medicine, Yokohama, Japan
    Neurosci Lett 275:175-8. 1999
    ..These results suggest that retina in diabetic mice has impaired capability of neurite regeneration in a normal glucose environment, but is adaptable to a high glucose environment in vitro...
  74. ncbi request reprint Perifoveal microcirculation before and after vitrectomy for diabetic cystoid macular edema
    K Kadonosono
    Department of Ophthalmology, Yokohama City University School of Medicine, Yokohama, Japan
    Am J Ophthalmol 130:740-4. 2000
    ....
  75. ncbi request reprint HLA class II genotyping of sarcoidosis patients in Hokkaido by PCR-RFLP
    M Ishihara
    Department of Ophthalmology, Yokohama City University School of Medicine, Japan
    Jpn J Ophthalmol 40:540-3. 1996
    ....
  76. ncbi request reprint Serotyping of Chlamydia trachomatis from inclusion conjunctivitis by polymerase chain reaction and restriction fragment length polymorphism analysis
    K Isobe
    Department of Ophthalmology, Yokohama City University School of Medicine, Japan
    Jpn J Ophthalmol 40:279-85. 1996
    ..One isolated strain could not be identified. The C. trachomatis which causes inclusion conjunctivitis in Japan appears to have a distribution of serovars similar to that of the sexually transmitted diseases...
  77. ncbi request reprint NF-kappa B and Sp1 regulate transcription of the human monocyte chemoattractant protein-1 gene
    A Ueda
    First Department of Internal Medicine, Yokohama City University School of Medicine, Japan
    J Immunol 153:2052-63. 1994
    ....
  78. ncbi request reprint MST/MLK2, a member of the mixed lineage kinase family, directly phosphorylates and activates SEK1, an activator of c-Jun N-terminal kinase/stress-activated protein kinase
    S I Hirai
    Department of Molecular Biology, Yokohama City University School of Medicine, 3 9, Fukuura, Kanazawa Ku, Yokohama 236, Japan
    J Biol Chem 272:15167-73. 1997
    ..Taken together, these results suggest that MLK family members are MAPK kinase kinases preferentially acting on the JNK/SAPK pathway...
  79. ncbi request reprint Iris neovascularization after vitrectomy combined with phacoemulsification and intraocular lens implantation for proliferative diabetic retinopathy
    K Kadonosono
    Department of Ophthalmology, Yokohama City University Medical Center, Japan
    Ophthalmic Surg Lasers 32:19-24. 2001
    ..To analyze the incidence of iris neovascularization after vitrectomy combined with phacoemulsification and intraocular lens (IOL) implantation using a small incision in the treatment of proliferative diabetic retinopathy (PDR)...
  80. ncbi request reprint Cloning and characterization of the T-box gene Tbx6 in Xenopus laevis
    H Uchiyama
    Graduate School of Integrated Science, Yokohama City University, 22 2 Seto, Kanazawa Ku, Yokohama 236 0027, Japan
    Dev Growth Differ 43:657-69. 2001
    ..These results suggest that Tbx6 is implicated in ventral mesoderm specification but is involved in muscle differentiation when acting together with the dorsalizing factor noggin...
  81. ncbi request reprint Treatment of retinal detachment resulting from myopic macular hole with internal limiting membrane removal
    K Kadonosono
    Department of Ophthalmology, Yokohama City University School of Medicine, 4 57 Urafune cho Minami ku, Yokohama 232 0024, Japan
    Am J Ophthalmol 131:203-7. 2001
    ..To examine the efficacy of vitrectomy with internal limiting membrane removal for retinal detachment resulting from a macular hole in highly myopic eyes...
  82. ncbi request reprint High prevalence of herpes simplex virus type 2 in acute retinal necrosis syndrome associated with herpes simplex virus in Japan
    N Itoh
    Department of Ophthalmology, Yokohama City University School of Medicine, Yokohama, Japan
    Am J Ophthalmol 129:404-5. 2000
    ..To determine the type of herpes simplex virus in acute retinal necrosis syndrome associated with herpes simplex virus...
  83. ncbi request reprint The proteolytic cleavage of protein kinase C isotypes, which generates kinase and regulatory fragments, correlates with Fas-mediated and 12-O-tetradecanoyl-phorbol-13-acetate-induced apoptosis
    K Mizuno
    Department of Molecular Biology, Yokohama City University School of Medicine, Yokohama, Japan
    Eur J Biochem 250:7-18. 1997
    ....
  84. ncbi request reprint Meiotic maturation induces animal-vegetal asymmetric distribution of aPKC and ASIP/PAR-3 in Xenopus oocytes
    M Nakaya
    Department of Molecular Biology, Yokohama City University School of Medicine, Yokohama 236-0004, Japan
    Development 127:5021-31. 2000
    ..These results suggest that meiotic maturation induces the animal-vegetal asymmetry of aPKC...
  85. pmc Clinical and epidemiological features of acute follicular conjunctivitis with special reference to that caused by herpes simplex virus type 1
    E Uchio
    Department of Ophthalmology, Yokohama City University School of Medicine, Yokohama, Japan
    Br J Ophthalmol 84:968-72. 2000
    ..3% of cases of epidemic keratoconjunctivitis (EKC) diagnosed clinically were caused by herpes simplex virus (HSV). Clinical and virological studies of patients with HSV conjunctivitis were carried out...
  86. ncbi request reprint Acute follicular conjunctivitis caused by adenovirus type 34
    E Uchio
    Department of Ophthalmology, Yokohama City University School of Medicine, Yokohama, Japan
    Am J Ophthalmol 128:680-6. 1999
    ..However, no case of acute conjunctivitis caused by adenovirus 34 has been reported. Recently, we encountered two cases of acute follicular conjunctivitis in which adenovirus 34 was isolated...
  87. pmc Serotyping of adenoviruses on conjunctival scrapings by PCR and sequence analysis
    S Takeuchi
    Department of Ophthalmology, Yokohama City University School of Medicine, 3 9 Fuku ura, Kanazawa Ku, Yokohama 236 0004, Japan
    J Clin Microbiol 37:1839-45. 1999
    ..These findings indicate that our newly developed PCR-sequence method is applicable for the detection and serotyping of human Ads...
  88. ncbi request reprint IL-12-encoding plasmid has a beneficial effect on spontaneous autoimmune disease in MRL/MP-lpr/lpr mice
    E Hagiwara
    Department of Internal Medicine, Yokohama City University of Medical School, Yokohama, Japan
    Cytokine 12:1035-41. 2000
    ..Serum IFN-gamma level was increased by inoculating IL-12 encoding plasmid, suggesting that the cytokine balance was skewed towards Th(1). The clinical implications of this suppression of autoimmune disease are also discussed...
  89. pmc Adenovirus strains of subgenus D associated with nosocomial infection as new etiological agents of epidemic keratoconjunctivitis in Japan
    S Takeuchi
    Department of Ophthalmology, Yokohama City University School of Medicine, Kanazawa Ku, Yokohama 236 0004, Japan
    J Clin Microbiol 37:3392-4. 1999
    ..PCR followed by restriction endonuclease analysis demonstrated that they were type 8. PCR followed by sequencing revealed a high homology rate between them and type 9...
  90. ncbi request reprint A domain containing the Cdc42/Rac interactive binding (CRIB) region of p65PAK inhibits transcriptional activation and cell transformation mediated by the Ras-Rac pathway
    S Osada
    Department of Molecular Biology, Yokohama City University School of Medicine, Kanazawa Ku, Japan
    FEBS Lett 404:227-33. 1997
    ..PAK/RD is a good tool to investigate the signaling pathways in which Rac and Cdc42 are involved...
  91. ncbi request reprint Differential activation of two JNK activators, MKK7 and SEK1, by MKN28-derived nonreceptor serine/threonine kinase/mixed lineage kinase 2
    S Hirai
    Department of Molecular Biology, Yokohama City University School of Medicine, 3 9 Fukuura, Kanazawa Ku, Yokohama 236, Japan
    J Biol Chem 273:7406-12. 1998
    ..These results provide a molecular aspect to the differential regulation of the two JNK activators by a variety of cellular stimuli...
  92. ncbi request reprint Positive and negative regulation of the transcription of the human protein kinase C beta gene
    Y S Niino
    Department of Molecular Biology, Tokyo Metropolitan Institute of Medical Science, Japan
    J Biol Chem 267:6158-63. 1992
    ..These results suggest the presence of multiple trans-acting factors that act on these positive and negative cis-acting elements and regulate the cell type-specific expression of the PKC beta gene...
  93. pmc MKBP, a novel member of the small heat shock protein family, binds and activates the myotonic dystrophy protein kinase
    A Suzuki
    Department of Molecular Biology, Yokohama City University School of Medicine, Kanazawa Ku, Yokohama 236, Japan
    J Cell Biol 140:1113-24. 1998
    ....
  94. ncbi request reprint Four new genotypes of adenovirus type 3 isolated from patients with conjunctivitis in Japan
    N Itoh
    Department of Ophthalmology, Yokohama City University School of Medicine, Yokohama, Japan
    J Med Virol 59:73-7. 1999
    ..This study demonstrates that the emergence of a new genotype of Ad3 may contribute to the replacement of the predominant serotype associated with adenovirus conjunctivitis in Japan...
  95. ncbi request reprint Staining of internal limiting membrane in macular hole surgery
    K Kadonosono
    Department of Ophthalmology, Yokohama City University School of Medicine, Yokohama, Japan
    Arch Ophthalmol 118:1116-8. 2000
    ..Our results show that this technique is safe and useful in visualizing the ILM, leading to the performance of successful removal of an ILM with least damage to the retina. Arch Ophthalmol. 2000;118:1116-1118..
  96. pmc Analysis of interferon-beta mRNA stability control after poly(I:C) stimulation using RNA metabolic labeling by ethynyluridine
    Kaito Abe
    Department of Cardiology, Yokohama City University School of Medicine, 3 9, Fukuura, Kanazawa Ku, Yokohama, Kanagawa 236 0004, Japan
    Biochem Biophys Res Commun 428:44-9. 2012
    ..The results indicate that this method of RNA metabolic labeling provides a general approach for the simultaneous analysis of transcriptional activity and mRNA stability coupled with transcriptional timing...
  97. ncbi request reprint A phorbol ester receptor/protein kinase, nPKC eta, a new member of the protein kinase C family predominantly expressed in lung and skin
    S Osada
    Department of Molecular Biology, Tokyo Metropolitan Institute of Medical Science, Japan
    J Biol Chem 265:22434-40. 1990
    ..These results clearly demonstrate the existence and the possible importance of nPKC eta as a member of the phorbol ester receptor/protein kinase, PKC, family...
  98. ncbi request reprint A novel phorbol ester receptor/protein kinase, nPKC, distantly related to the protein kinase C family
    S Ohno
    Department of Molecular Biology, Tokyo Metropolitan Institute of Medical Science, Japan
    Cell 53:731-41. 1988
    ..These results suggest an additional signaling pathway involving nPKC...
  99. ncbi request reprint A protein kinase Cdelta-binding protein SRBC whose expression is induced by serum starvation
    Y Izumi
    Department of Molecular Biology, Yokohama City University School of Medicine, 3 9, Fuku ura, Kanazawa Ku, Yokohama 236, Japan
    J Biol Chem 272:7381-9. 1997
    ..These results suggest that SRBC serves as a substrate and/or receptor for PKC and might be involved in the control of cell growth mediated by PKC...
  100. pmc A metalloprotease-disintegrin, MDC9/meltrin-gamma/ADAM9 and PKCdelta are involved in TPA-induced ectodomain shedding of membrane-anchored heparin-binding EGF-like growth factor
    Y Izumi
    Department of Molecular Biology, Yokohama City University School of Medicine 3 9, Fuku ura, Kanagawa ku, Yokohama 236 0004, Japan
    EMBO J 17:7260-72. 1998
    ..These results suggest that MDC9 and PKCdelta are involved in the stimulus-coupled shedding of the proHB-EGF ectodomain...