Research Topics
| Rinji AkadaSummaryAffiliation: Yamaguchi University Country: Japan Publications
| Collaborators
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Detail Information
Publications
DNA extraction method for screening yeast clones by PCRR Akada
Department of Applied Chemistry and Chemical Engineering, Faculty of Engineering, Yamaguchi University, Ube, Japan
Biotechniques 28:668-70, 672, 674. 2000..25% SDS solution, mixed vigorously and centrifuged. The supernatant can be directly used as a template after dilution to give an SDS concentration of less than 0.01% in the final PCR mixture...- Use of a YAP1 overexpression cassette conferring specific resistance to cerulenin and cycloheximide as an efficient selectable marker in the yeast Saccharomyces cerevisiaeRinji Akada
Department of Applied Chemistry and Chemical Engineering, Faculty of Engineering, Yamaguchi University, Tokiwadai, Ube 755 8611, Japan
Yeast 19:17-28. 2002..The PGKp-YAP1 marker was used to disrupt the LYS2 gene and to transform an industrial yeast strain, indicating that this marker can be used for efficient and reliable gene manipulations in any Saccharomyces cerevisiae strain... - Sets of integrating plasmids and gene disruption cassettes containing improved counter-selection markers designed for repeated use in budding yeastRinji Akada
Department of Applied Chemistry and Chemical Engineering, Faculty of Engineering, Yamaguchi University, Tokiwadai, Ube 755 8611, Japan
Yeast 19:393-402. 2002..The counter-selectable integrating plasmids and the gene disruption cassettes can allow the markers to be used repeatedly for yeast gene manipulations... 
High-temperature ethanol fermentation and transformation with linear DNA in the thermotolerant yeast Kluyveromyces marxianus DMKU3-1042Sanom Nonklang
Department of Applied Molecular Bioscience, Yamaguchi University Graduate School of Medicine, Tokiwadai, Ube 755 8611, Japan
Appl Environ Microbiol 74:7514-21. 2008..Our results demonstrate that K. marxianus DMKU3-1042 can be an alternative cost-effective bioethanol producer and a host for transformation with linear DNA by use of S. cerevisiae-based molecular genetic tools...- Direct mating between diploid sake strains of Saccharomyces cerevisiaeShinji Hashimoto
Department of Applied Chemistry and Chemical Engineering, Faculty of Engineering, Yamaguchi University, Tokiwadai, Ube, 755-8611, Japan
Appl Microbiol Biotechnol 69:689-96. 2006..Isolation of mating-competent auxotrophic mutants directly from industrial yeast strains allows crossbreeding to construct polyploids suitable for industrial use without dependence on sporulation... 
Random and targeted gene integrations through the control of non-homologous end joining in the yeast Kluyveromyces marxianusBabiker M A Abdel-Banat
Department of Applied Molecular Bioscience, Yamaguchi University Graduate School of Medicine, Tokiwadai, Ube 755 8611, Japan
Yeast 27:29-39. 2010..These results indicate that the highly efficient NHEJ pathway can be utilized with random gene disruption techniques such as transposon mutagenesis and plasmid-free gene manipulations in K. marxianus...- High-temperature fermentation: how can processes for ethanol production at high temperatures become superior to the traditional process using mesophilic yeast?Babiker M A Abdel-Banat
Department of Applied Molecular Bioscience, Yamaguchi University Graduate School of Medicine, Tokiwadai, Ube, Japan
Appl Microbiol Biotechnol 85:861-7. 2010.... - Fusion PCR via novel overlap sequencesKamonchai Cha-Aim
Department of Applied Molecular Bioscience, Yamaguchi University Graduate School of Medicine, Ube, Japan
Methods Mol Biol 852:97-110. 2012..We describe how these overlap sequences can be used for fusion DNA construction, in-frame gene fusion, and cloning in yeast... - Identification of genes that enhance cellulase protein production in yeastTakao Kitagawa
Department of Applied Molecular Bioscience, Yamaguchi University Graduate School of Medicine, Tokiwadai, Ube 755 8611, Japan
J Biotechnol 151:194-203. 2011..Our genome-wide analyses using the yeast deletion strain collection identified useful genes that allow efficient expression of cellulase... - PCR-mediated seamless gene deletion and marker recycling in Saccharomyces cerevisiaeRinji Akada
Department of Applied Chemistry and Chemical Engineering, Faculty of Engineering, Yamaguchi University, 2 16 1 Tokiwadai, Ube 755 8611, Japan
Yeast 23:399-405. 2006..All the selected colonies had lost URA3 precisely by recombination between the repeats, resulting in his3 deletion without any extraneous sequences left behind in the chromosome... - Genome-wide analysis of cellular response to bacterial genotoxin CdtB in yeastTakao Kitagawa
Department of Applied Molecular Bioscience, Division of Engineering, Yamaguchi University Graduate School of Medicine, Tokiwadai, Ube 755 8611, Japan
Infect Immun 75:1393-402. 2007..Our present results provide insights into how CdtB pathogenesis is linked to eukaryotic cellular functions... - Self-cloning yeast strains containing novel FAS2 mutations produce a higher amount of ethyl caproate in Japanese sakeKazuo Aritomi
Yamaguchi Prefectural Industrial Technology Institute, Ube, Japan
Biosci Biotechnol Biochem 68:206-14. 2004..Interestingly, these mutants only showed detectable cerulenin resistance. These 'self-cloning' yeast strains should be acceptable to the public because they can improve sake quality without the presence of extraneous DNA sequences... - Construction of flocculent Kluyveromyces marxianus strains suitable for high-temperature ethanol fermentationSanom Nonklang
Department of Applied Molecular Bioscience, Yamaguchi University Graduate School of Medicine, Tokiwadai, Ube, Japan
Biosci Biotechnol Biochem 73:1090-5. 2009..The K. marxianus flocculating strains we generated should be useful in the future development of cost-effective fed-batch and continuous fermentation systems at high temperatures... - Construction of a URA3 deletion strain from the allotetraploid bottom-fermenting yeast Saccharomyces pastorianusNobutada Murakami
Department of Applied Molecular Bioscience, Graduate School of Medicine, Yamaguchi University, Ube, Japan
Yeast 29:155-65. 2012..The ATF1-overexpressing transformant produced significantly more acetate esters than the parent strain. The constructed ura3∆ lager strain will be a useful host for constructing strains of relevance to brewing... - Reliable fusion PCR mediated by GC-rich overlap sequencesKamonchai Cha-Aim
Department of Applied Chemistry, Graduate School of Science and Engineering, Yamaguchi University, Tokiwadai, Ube 755 8611, Japan
Gene 434:43-9. 2009..Novel poly G or C stretches showed strong and also specific annealing to the complementary sequences in the fusion PCR. This DNA fusion method is thus both a simple and versatile recombinant DNA technique... - Screening of drugs that suppress Ste11 MAPKKK activation in yeast identified a c-Abl tyrosine kinase inhibitorTakao Kitagawa
Department of Applied Molecular Bioscience, Yamaguchi University Graduate School of Medicine, Ube, Japan
Biosci Biotechnol Biochem 71:772-82. 2007..These results suggest that drug screening in yeast can identify human tyrosine kinase inhibitors and other drugs for human diseases... - Isolation of auxotrophic mutants of diploid industrial yeast strains after UV mutagenesisShinji Hashimoto
Department of Applied Chemistry and Chemical Engineering, Faculty of Engineering, Yamaguchi University, Tokiwadai, Ube 755-8611, Japan
Appl Environ Microbiol 71:312-9. 2005..We argue that the generally recognized difficulty of isolating auxotrophic mutants of diploid industrial yeast strains is misleading and that genetic techniques used for haploid laboratory strains are applicable for this purpose... - Copper-dependent production of a Pycnoporus coccineus extracellular laccase in Aspergillus oryzae and Saccharomyces cerevisiaeHisashi Hoshida
Department of Applied Chemistry and Chemical Engineering, Faculty of Engineering, Yamaguchi University, Tokiwadai, Ube, Japan
Biosci Biotechnol Biochem 69:1090-7. 2005..Our results indicate that a high copper concentration is required for the production of active laccase in heterologous hosts and that the copper is required for a post-transcriptional process... - Helicobacter pylori CagA inhibits endocytosis of cytotoxin VacA in host cellsJunko K Akada
Department of Biochemistry and Functional Proteomics, Yamaguchi University Graduate School of Medicine, Ube, Yamaguchi, Japan
Dis Model Mech 3:605-17. 2010..This mechanism might allow H. pylori to balance damage to one population of host cells with the preservation of another, allowing for persistent infection... - Isolation of a novel mutant strain of Saccharomyces cerevisiae by an ethyl methane sulfonate-induced mutagenesis approach as a high producer of bioethanolMohsen Mobini-Dehkordi
Department of Biology, Faculty of Sciences, University of Isfahan, Azadi Sq, Daneshgah St, 81746 73441, Isfahan, I R Iran
J Biosci Bioeng 105:403-8. 2008..Among many isolated mutant strains, there were six isolated colonies that grew on ALP medium supplemented with 10% (v/v) ethanol and one of them produced bioethanol 17.3% more than the wild type... - Loss of heterozygosity is induced in Candida albicans by ultraviolet irradiationYukinobu Takagi
Division of Integrated Life Sciences, Graduate School of Biostudies, Kyoto University, Oiwake cho, Kitashirakawa, Sakyo ku, Kyoto, 606 8502, Japan
Appl Microbiol Biotechnol 77:1073-82. 2008..These results suggest that high efficiency LOH caused by UV irradiation could be a prominent tool for gene analyses in C. albicans...