Rinji Akada

Summary

Affiliation: Yamaguchi University
Country: Japan

Publications

  1. ncbi request reprint DNA extraction method for screening yeast clones by PCR
    R Akada
    Department of Applied Chemistry and Chemical Engineering, Faculty of Engineering, Yamaguchi University, Ube, Japan
    Biotechniques 28:668-70, 672, 674. 2000
  2. ncbi request reprint Use of a YAP1 overexpression cassette conferring specific resistance to cerulenin and cycloheximide as an efficient selectable marker in the yeast Saccharomyces cerevisiae
    Rinji Akada
    Department of Applied Chemistry and Chemical Engineering, Faculty of Engineering, Yamaguchi University, Tokiwadai, Ube 755 8611, Japan
    Yeast 19:17-28. 2002
  3. ncbi request reprint Sets of integrating plasmids and gene disruption cassettes containing improved counter-selection markers designed for repeated use in budding yeast
    Rinji Akada
    Department of Applied Chemistry and Chemical Engineering, Faculty of Engineering, Yamaguchi University, Tokiwadai, Ube 755 8611, Japan
    Yeast 19:393-402. 2002
  4. pmc High-temperature ethanol fermentation and transformation with linear DNA in the thermotolerant yeast Kluyveromyces marxianus DMKU3-1042
    Sanom Nonklang
    Department of Applied Molecular Bioscience, Yamaguchi University Graduate School of Medicine, Tokiwadai, Ube 755 8611, Japan
    Appl Environ Microbiol 74:7514-21. 2008
  5. ncbi request reprint Direct mating between diploid sake strains of Saccharomyces cerevisiae
    Shinji Hashimoto
    Department of Applied Chemistry and Chemical Engineering, Faculty of Engineering, Yamaguchi University, Tokiwadai, Ube, 755 8611, Japan
    Appl Microbiol Biotechnol 69:689-96. 2006
  6. doi request reprint Designed construction of recombinant DNA at the ura3Δ0 locus in the yeast Saccharomyces cerevisiae
    Tomoaki Fukunaga
    Department of Applied Molecular Bioscience, Graduate School of Medicine, Yamaguchi University, Tokiwadai, Ube, Japan
    Yeast 30:243-53. 2013
  7. doi request reprint Random and targeted gene integrations through the control of non-homologous end joining in the yeast Kluyveromyces marxianus
    Babiker M A Abdel-Banat
    Department of Applied Molecular Bioscience, Yamaguchi University Graduate School of Medicine, Tokiwadai, Ube 755 8611, Japan
    Yeast 27:29-39. 2010
  8. ncbi request reprint High-temperature fermentation: how can processes for ethanol production at high temperatures become superior to the traditional process using mesophilic yeast?
    Babiker M A Abdel-Banat
    Department of Applied Molecular Bioscience, Yamaguchi University Graduate School of Medicine, Tokiwadai, Ube, Japan
    Appl Microbiol Biotechnol 85:861-7. 2010
  9. doi request reprint Fusion PCR via novel overlap sequences
    Kamonchai Cha-Aim
    Department of Applied Molecular Bioscience, Yamaguchi University Graduate School of Medicine, Ube, Japan
    Methods Mol Biol 852:97-110. 2012
  10. doi request reprint Identification of genes that enhance cellulase protein production in yeast
    Takao Kitagawa
    Department of Applied Molecular Bioscience, Yamaguchi University Graduate School of Medicine, Tokiwadai, Ube 755 8611, Japan
    J Biotechnol 151:194-203. 2011

Collaborators

Detail Information

Publications26

  1. ncbi request reprint DNA extraction method for screening yeast clones by PCR
    R Akada
    Department of Applied Chemistry and Chemical Engineering, Faculty of Engineering, Yamaguchi University, Ube, Japan
    Biotechniques 28:668-70, 672, 674. 2000
    ..25% SDS solution, mixed vigorously and centrifuged. The supernatant can be directly used as a template after dilution to give an SDS concentration of less than 0.01% in the final PCR mixture...
  2. ncbi request reprint Use of a YAP1 overexpression cassette conferring specific resistance to cerulenin and cycloheximide as an efficient selectable marker in the yeast Saccharomyces cerevisiae
    Rinji Akada
    Department of Applied Chemistry and Chemical Engineering, Faculty of Engineering, Yamaguchi University, Tokiwadai, Ube 755 8611, Japan
    Yeast 19:17-28. 2002
    ..The PGKp-YAP1 marker was used to disrupt the LYS2 gene and to transform an industrial yeast strain, indicating that this marker can be used for efficient and reliable gene manipulations in any Saccharomyces cerevisiae strain...
  3. ncbi request reprint Sets of integrating plasmids and gene disruption cassettes containing improved counter-selection markers designed for repeated use in budding yeast
    Rinji Akada
    Department of Applied Chemistry and Chemical Engineering, Faculty of Engineering, Yamaguchi University, Tokiwadai, Ube 755 8611, Japan
    Yeast 19:393-402. 2002
    ..The counter-selectable integrating plasmids and the gene disruption cassettes can allow the markers to be used repeatedly for yeast gene manipulations...
  4. pmc High-temperature ethanol fermentation and transformation with linear DNA in the thermotolerant yeast Kluyveromyces marxianus DMKU3-1042
    Sanom Nonklang
    Department of Applied Molecular Bioscience, Yamaguchi University Graduate School of Medicine, Tokiwadai, Ube 755 8611, Japan
    Appl Environ Microbiol 74:7514-21. 2008
    ..Our results demonstrate that K. marxianus DMKU3-1042 can be an alternative cost-effective bioethanol producer and a host for transformation with linear DNA by use of S. cerevisiae-based molecular genetic tools...
  5. ncbi request reprint Direct mating between diploid sake strains of Saccharomyces cerevisiae
    Shinji Hashimoto
    Department of Applied Chemistry and Chemical Engineering, Faculty of Engineering, Yamaguchi University, Tokiwadai, Ube, 755 8611, Japan
    Appl Microbiol Biotechnol 69:689-96. 2006
    ..Isolation of mating-competent auxotrophic mutants directly from industrial yeast strains allows crossbreeding to construct polyploids suitable for industrial use without dependence on sporulation...
  6. doi request reprint Designed construction of recombinant DNA at the ura3Δ0 locus in the yeast Saccharomyces cerevisiae
    Tomoaki Fukunaga
    Department of Applied Molecular Bioscience, Graduate School of Medicine, Yamaguchi University, Tokiwadai, Ube, Japan
    Yeast 30:243-53. 2013
    ..coli. Interestingly, we unexpectedly found that several G/C-rich sequences used for fusion PCR lowered gene expression when located adjacent to the start codon...
  7. doi request reprint Random and targeted gene integrations through the control of non-homologous end joining in the yeast Kluyveromyces marxianus
    Babiker M A Abdel-Banat
    Department of Applied Molecular Bioscience, Yamaguchi University Graduate School of Medicine, Tokiwadai, Ube 755 8611, Japan
    Yeast 27:29-39. 2010
    ..These results indicate that the highly efficient NHEJ pathway can be utilized with random gene disruption techniques such as transposon mutagenesis and plasmid-free gene manipulations in K. marxianus...
  8. ncbi request reprint High-temperature fermentation: how can processes for ethanol production at high temperatures become superior to the traditional process using mesophilic yeast?
    Babiker M A Abdel-Banat
    Department of Applied Molecular Bioscience, Yamaguchi University Graduate School of Medicine, Tokiwadai, Ube, Japan
    Appl Microbiol Biotechnol 85:861-7. 2010
    ....
  9. doi request reprint Fusion PCR via novel overlap sequences
    Kamonchai Cha-Aim
    Department of Applied Molecular Bioscience, Yamaguchi University Graduate School of Medicine, Ube, Japan
    Methods Mol Biol 852:97-110. 2012
    ..We describe how these overlap sequences can be used for fusion DNA construction, in-frame gene fusion, and cloning in yeast...
  10. doi request reprint Identification of genes that enhance cellulase protein production in yeast
    Takao Kitagawa
    Department of Applied Molecular Bioscience, Yamaguchi University Graduate School of Medicine, Tokiwadai, Ube 755 8611, Japan
    J Biotechnol 151:194-203. 2011
    ..Our genome-wide analyses using the yeast deletion strain collection identified useful genes that allow efficient expression of cellulase...
  11. ncbi request reprint PCR-mediated seamless gene deletion and marker recycling in Saccharomyces cerevisiae
    Rinji Akada
    Department of Applied Chemistry and Chemical Engineering, Faculty of Engineering, Yamaguchi University, 2 16 1 Tokiwadai, Ube 755 8611, Japan
    Yeast 23:399-405. 2006
    ..All the selected colonies had lost URA3 precisely by recombination between the repeats, resulting in his3 deletion without any extraneous sequences left behind in the chromosome...
  12. pmc Genome-wide analysis of cellular response to bacterial genotoxin CdtB in yeast
    Takao Kitagawa
    Department of Applied Molecular Bioscience, Division of Engineering, Yamaguchi University Graduate School of Medicine, Tokiwadai, Ube 755 8611, Japan
    Infect Immun 75:1393-402. 2007
    ..Our present results provide insights into how CdtB pathogenesis is linked to eukaryotic cellular functions...
  13. doi request reprint Non-homologous end joining-mediated functional marker selection for DNA cloning in the yeast Kluyveromyces marxianus
    Hisashi Hoshida
    Department of Applied Molecular Bioscience, Graduate School of Medicine, Yamaguchi University, Tokiwadai, Ube, Japan
    Yeast 31:29-46. 2014
    ..The one-step DNA cloning method developed here is a relatively simple and reliable procedure among the DNA cloning systems developed to date...
  14. ncbi request reprint Construction of flocculent Kluyveromyces marxianus strains suitable for high-temperature ethanol fermentation
    Sanom Nonklang
    Department of Applied Molecular Bioscience, Yamaguchi University Graduate School of Medicine, Tokiwadai, Ube, Japan
    Biosci Biotechnol Biochem 73:1090-5. 2009
    ..The K. marxianus flocculating strains we generated should be useful in the future development of cost-effective fed-batch and continuous fermentation systems at high temperatures...
  15. ncbi request reprint Self-cloning yeast strains containing novel FAS2 mutations produce a higher amount of ethyl caproate in Japanese sake
    Kazuo Aritomi
    Yamaguchi Prefectural Industrial Technology Institute, Ube, Japan
    Biosci Biotechnol Biochem 68:206-14. 2004
    ..Interestingly, these mutants only showed detectable cerulenin resistance. These 'self-cloning' yeast strains should be acceptable to the public because they can improve sake quality without the presence of extraneous DNA sequences...
  16. ncbi request reprint Identification of auxotrophic mutants of the yeast Kluyveromyces marxianus by non-homologous end joining-mediated integrative transformation with genes from Saccharomyces cerevisiae
    Tohru Yarimizu
    Department of Applied Molecular Bioscience, Graduate School of Medicine, Yamaguchi University, Tokiwadai, Ube, Japan
    Yeast 30:485-500. 2013
    ..cerevisiae genes as selective markers, NHEJ-mediated integrative transformation with PCR-amplified DNA is an attractive system for facilitating genetic analyses in the yeast K. marxianus...
  17. doi request reprint N-Glycosylation deficiency enhanced heterologous production of a Bacillus licheniformis thermostable α-amylase in Saccharomyces cerevisiae
    Hisashi Hoshida
    Department of Applied Molecular Bioscience, Graduate School of Medicine, Yamaguchi University, 2 16 1 Tokiwadai, Ube, Japan
    Appl Microbiol Biotechnol 97:5473-82. 2013
    ..To achieve higher AmyL production in yeast, all six N-glycosylation sites of AmyL were mutated. In wild-type yeast, production of the resulting non-glycosylated form of AmyL was threefold higher than that of the glycosylated form...
  18. doi request reprint Construction of a URA3 deletion strain from the allotetraploid bottom-fermenting yeast Saccharomyces pastorianus
    Nobutada Murakami
    Department of Applied Molecular Bioscience, Graduate School of Medicine, Yamaguchi University, Ube, Japan
    Yeast 29:155-65. 2012
    ..The ATF1-overexpressing transformant produced significantly more acetate esters than the parent strain. The constructed ura3∆ lager strain will be a useful host for constructing strains of relevance to brewing...
  19. ncbi request reprint Reliable fusion PCR mediated by GC-rich overlap sequences
    Kamonchai Cha-Aim
    Department of Applied Chemistry, Graduate School of Science and Engineering, Yamaguchi University, Tokiwadai, Ube 755 8611, Japan
    Gene 434:43-9. 2009
    ..Novel poly G or C stretches showed strong and also specific annealing to the complementary sequences in the fusion PCR. This DNA fusion method is thus both a simple and versatile recombinant DNA technique...
  20. ncbi request reprint Screening of drugs that suppress Ste11 MAPKKK activation in yeast identified a c-Abl tyrosine kinase inhibitor
    Takao Kitagawa
    Department of Applied Molecular Bioscience, Yamaguchi University Graduate School of Medicine, Ube, Japan
    Biosci Biotechnol Biochem 71:772-82. 2007
    ..These results suggest that drug screening in yeast can identify human tyrosine kinase inhibitors and other drugs for human diseases...
  21. pmc Isolation of auxotrophic mutants of diploid industrial yeast strains after UV mutagenesis
    Shinji Hashimoto
    Department of Applied Chemistry and Chemical Engineering, Faculty of Engineering, Yamaguchi University, Tokiwadai, Ube 755 8611, Japan
    Appl Environ Microbiol 71:312-9. 2005
    ..We argue that the generally recognized difficulty of isolating auxotrophic mutants of diploid industrial yeast strains is misleading and that genetic techniques used for haploid laboratory strains are applicable for this purpose...
  22. ncbi request reprint Copper-dependent production of a Pycnoporus coccineus extracellular laccase in Aspergillus oryzae and Saccharomyces cerevisiae
    Hisashi Hoshida
    Department of Applied Chemistry and Chemical Engineering, Faculty of Engineering, Yamaguchi University, Tokiwadai, Ube, Japan
    Biosci Biotechnol Biochem 69:1090-7. 2005
    ..Our results indicate that a high copper concentration is required for the production of active laccase in heterologous hosts and that the copper is required for a post-transcriptional process...
  23. ncbi request reprint Minimum GC-rich sequences for overlap extension PCR and primer annealing
    Mikiko Nakamura
    Innovation Center, Yamaguchi University, Ube, Japan
    Methods Mol Biol 1116:165-81. 2014
    ..Here, we describe universal GC-rich annealing sequences useful for overlap extension PCR and simple in-frame addition of desired sequences. ..
  24. ncbi request reprint Helicobacter pylori CagA inhibits endocytosis of cytotoxin VacA in host cells
    Junko K Akada
    Department of Biochemistry and Functional Proteomics, Yamaguchi University Graduate School of Medicine, Ube, Yamaguchi, Japan
    Dis Model Mech 3:605-17. 2010
    ..This mechanism might allow H. pylori to balance damage to one population of host cells with the preservation of another, allowing for persistent infection...
  25. doi request reprint Isolation of a novel mutant strain of Saccharomyces cerevisiae by an ethyl methane sulfonate-induced mutagenesis approach as a high producer of bioethanol
    Mohsen Mobini-Dehkordi
    Department of Biology, Faculty of Sciences, University of Isfahan, Azadi Sq, Daneshgah St, 81746 73441, Isfahan, I R Iran
    J Biosci Bioeng 105:403-8. 2008
    ..Among many isolated mutant strains, there were six isolated colonies that grew on ALP medium supplemented with 10% (v/v) ethanol and one of them produced bioethanol 17.3% more than the wild type...
  26. ncbi request reprint Loss of heterozygosity is induced in Candida albicans by ultraviolet irradiation
    Yukinobu Takagi
    Division of Integrated Life Sciences, Graduate School of Biostudies, Kyoto University, Oiwake cho, Kitashirakawa, Sakyo ku, Kyoto, 606 8502, Japan
    Appl Microbiol Biotechnol 77:1073-82. 2008
    ..These results suggest that high efficiency LOH caused by UV irradiation could be a prominent tool for gene analyses in C. albicans...