Research Topics
| Fumio WatanabeSummaryCountry: Japan Publications
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Detail Information
Publications
Adenosylcobalamin-dependent methylmalonyl-CoA mutase isozymes in the photosynthetic protozoon Euglena gracilis ZF Watanabe
Department of Food and Nutrition, Kochi Women s University, Japan
Microbiology 142:2631-4. 1996..These results provide evidence that propionate-adapted Euglena contains two MCM isozymes. The induced mutase (M(r) 17,000) permits photoassimilation of propionate...
Pseudovitamin B(12) is the predominant cobamide of an algal health food, spirulina tabletsF Watanabe
Department of Health Science, Kochi Women s University, Kochi 780 8515, Japan
J Agric Food Chem 47:4736-41. 1999..leichmannii as a test organism and the binding of vitamin B(12) to the intrinsic factor...
Dried green and purple lavers (Nori) contain substantial amounts of biologically active vitamin B(12) but less of dietary iodine relative to other edible seaweedsF Watanabe
Department of Health Science, Kochi Women s University, Japan
J Agric Food Chem 47:2341-3. 1999..These results indicate that the dried lavers (nori) are the most excellent source of vitamin B(12) among edible seaweeds, especially for strict vegetarians...
Characterization of a vitamin B12 compound in the edible purple laver, Porphyra yezoensisF Watanabe
Department of Health Science, Kochi Women s University, Japan
Biosci Biotechnol Biochem 64:2712-5. 2000..The silica gel 60 TLC and reversed-phase HPLC patterns of the purified pink-colored compound were identical to those of authentic vitamin B12, but not to those of vitamin B12 analogues inactive for humans...
Inactive corrinoid-compound significantly decreases in Spirulina platensis grown in a cobalt-deficient mediumF Watanabe
Department of Health Science, Kochi Women s University, Kochi 780 8515, Japan
J Agric Food Chem 49:5685-8. 2001..These results indicate that the algal cells grown in the absence of CoSO(4) are suitable for use of human health foods because the inactive corrinoid-compound can be reduced significantly...
Characterization and bioavailability of vitamin B12-compounds from edible algaeFumio Watanabe
Department of Health Science, Kochi Women s University, Kochi 780 8515, Japan
J Nutr Sci Vitaminol (Tokyo) 48:325-31. 2002..Pseudovitamin B12 (an inactive corrinoid) predominated in the spirulina tablets, which are not suitable for use as a vitamin B12 source, especially for vegetarians. algal health food, bioavailability, cobalamin, edible algae, vitamin B12..
Purification and characterization of a corrinoid compound from Chlorella tablets as an algal health foodHiromi Kittaka-Katsura
Department of Health Science, Hiroshima Prefectural Women's University, Hiroshima 734-8558, Japan
J Agric Food Chem 50:4994-7. 2002..The purified corrinoid compound was identified as vitamin B12, on the basis of silica gel 60 TLC, C18 reversed-phase HPLC, 1H NMR spectroscopy, and UV-Vis spectroscopy...
Purification and characterization of homodimeric methylmalonyl-CoA mutase from Sinorhizobium melilotiEmi Miyamoto
Department of Health Science, Kochi Women's University, 780-8515, Kochi, Japan
Arch Microbiol 180:151-4. 2003..0 kDa, indicating that the S. meliloti enzyme is composed of two identical subunits. The NH(2)-terminal sequence was identical to that predicted from the bhbA nucleotide sequence. Monovalent cations were required for enzyme activity...
Purification and characterization of methylmalonyl-CoA mutase from a photosynthetic coccolithophorid alga, Pleurochrysis carteraeEmi Miyamoto
Department of Health Science, Kochi Women's University, 5-15 Eikokuji-cho, Kochi 780-8515, Japan
Comp Biochem Physiol B Biochem Mol Biol 138:163-7. 2004..carterae enzyme occurs as a homodimer. Some properties of methylmalonyl-CoA mutase from P. carterae were studied...
Characterization of corrinoid compounds from a Japanese black tea (Batabata-cha) fermented by bacteriaHiromi Kittaka-Katsura
Department of Food and Nutrition, Kyoto Women s University, Kyoto 605 8501, Japan
J Agric Food Chem 52:909-11. 2004..The results indicate that the vitamin B(12) found in the fermented black tea is bioavailable in mammals...
Occurrence of vitamin B12 in green, blue, red, and black tea leavesHiromi Kittaka-Katsura
Department of Food and Nutrition, Kyoto Women s Untiversity, Kyoto 605 8501, Japan
J Nutr Sci Vitaminol (Tokyo) 50:438-40. 2004....
Purification and characterization of methylmalonyl-CoA mutase from a methanol-utilizing bacterium, Methylobacterium extorquens NR-1Emi Miyamoto
Department of Health Science, Kochi Women's University, Japan
J Nutr Sci Vitaminol (Tokyo) 48:242-6. 2002..extorquens NR-1 enzyme showed no significant homology to those of the enzyme from other species. Some enzymological properties of the M. extorquens NR-1 enzyme were studied...
Uptake and physiological function of vitamin B12 in a photosynthetic unicellular coccolithophorid alga, Pleurochrysis carteraeEmi Miyamoto
Department of Health Science, Kochi Women's University, Japan
Biosci Biotechnol Biochem 66:195-8. 2002..2% of the vitamin B12 accumulated by the algal cells were recovered in the macromolecular fractions with Mr of 150 kDa, although the remaining vitamin B12 was found only in free vitamin B12 fractions...
Loss of vitamin B(12) in fish (round herring) meats during various cooking treatmentsMichiko Nishioka
Department of Health Science, University of Kochi, Kochi, Japan
J Nutr Sci Vitaminol (Tokyo) 57:432-6. 2011..Retention of vitamin B(12) was not dependent on vacuum or temperature (or both) used in the vacuum-packed pouch cooking...
Vitamin B12 sources and bioavailabilityFumio Watanabe
School of Agricultural, Biological and Environmental Sciences, Faculty of Agriculture, Tottori University, 4 101 Koyama Minami, Tottori 680 8553, Japan
Exp Biol Med (Maywood) 232:1266-74. 2007..Fortified breakfast cereals are a particularly valuable source of vitamin B(12) for vegans and elderly people. Production of some vitamin B(12)-enriched vegetables is also being devised...
Hypoxia-induced megaloblastosis in vitamin B12-deficient ratsShuhei Ebara
Department of Applied Biological Chemistry, Osaka Prefecture University, Sakai, Osaka, Japan
Br J Nutr 89:441-4. 2003..These results indicate that a megaloblastic anaemia-like symptom is induced when the vitamin B12-deficient rats are exposed to hypoxia...
Values of water-soluble vitamins in blood and urine of Japanese young men and women consuming a semi-purified diet based on the Japanese Dietary Reference IntakesKatsumi Shibata
School of Human Cultures, The University of Shiga Prefecture, Hikone, Shiga 522 8533, Japan
J Nutr Sci Vitaminol (Tokyo) 51:319-28. 2005..In future, an experiment with various age groups and re-evaluation by repeated experiments will provide more accurate values...
Purification and characterization of a corrinoid-compound in an edible cyanobacterium Aphanizomenon flos-aquae as a nutritional supplementary foodEmi Miyamoto
Department of Health and Nutrition, Nagasaki International University, Sasebo, Japan
J Agric Food Chem 54:9604-7. 2006..The results suggest that the Aphanizomenon cells are not suitable for use as a vitamin B12 source, especially in vegans...
Effect of vitamin B12-enriched thraustochytrids on the population growth of rotifersMasahiro Hayashi
Department of Biological Production and Environmental Science, Faculty of Agriculture, University of Miyazaki, Japan
Biosci Biotechnol Biochem 71:222-5. 2007..Vitamin B12-enriched thraustochytrids, strain mh0186, enhanced the population growth of rotifers fed on the cells as sole feed...
Purification and characterization of corrinoid-compounds from the dried powder of an edible cyanobacterium, Nostoc commune (Ishikurage)Fumio Watanabe
School of Agricultural, Biological, and Environmental Sciences, Tottori University, Tottori 680 8553, Japan
J Nutr Sci Vitaminol (Tokyo) 53:183-6. 2007..These results suggest that the bacterial cells are not suitable for use as a vitamin B12 source, especially in vegetarians...
Vitamin B12 deficiency results in the abnormal regulation of serine dehydratase and tyrosine aminotransferase activities correlated with impairment of the adenylyl cyclase system in rat liverShuhei Ebara
School of Human Science and Environment, University of Hyogo, Himeji, Hyogo 670 0092, Japan
Br J Nutr 99:503-10. 2008..These results suggest that vitamin B(12) deficiency results in decreases in SDH and TAT activities correlated with the impairment of the glucagon signal transduction through the activation of the adenylyl cyclase system in the liver...
Occurrence of coenzyme forms of vitamin B12 in a cultured purple laver (Porphyla yezoensis)Shigeo Takenaka
Department of Veterinary Sciences, Osaka Prefecture University, Sakai, Japan
Biosci Biotechnol Biochem 67:2480-2. 2003..Coenzyme forms of vitamin B(12) (about 60% of the total vitamin B(12)) were found in the cultured purple laver aseptically, which may have the ability to biosynthesize the coenzymes...
