Shin ichi Ishiwata

Summary

Affiliation: Waseda University
Country: Japan

Publications

  1. ncbi request reprint Microscopic analysis of polymerization dynamics with individual actin filaments
    Ikuko Fujiwara
    Department of Physics, School of Science and Engineering, Waseda University, 3 4 1 Okubo, Shinjuku ku, Tokyo 169 8555, Japan
    Nat Cell Biol 4:666-73. 2002
  2. ncbi request reprint Activated protein C via PAR1 receptor regulates survival of neurons under conditions of glutamate excitotoxicity
    L R Gorbacheva
    Biology Faculty, Lomonosov Moscow State University, Moscow, 119899, Russia
    Biochemistry (Mosc) 73:717-24. 2008
  3. doi request reprint Contractile system of muscle as an auto-oscillator
    Shin ichi Ishiwata
    Department of Physics, Faculty of Science and Engineering, Waseda University, 3 4 1 Okubo, Shinjuku ku, Tokyo, Japan
    Prog Biophys Mol Biol 105:187-98. 2011
  4. ncbi request reprint Regulation of muscle contraction by Ca2+ and ADP: focusing on the auto-oscillation (SPOC)
    Shin ichi Ishiwata
    Department of Physics, Faculty of Science and Engineering, Waseda University, 3 4 1 Okubo, Shinjuku ku, Tokyo 169 8555, Japan
    Adv Exp Med Biol 592:341-58. 2007
  5. ncbi request reprint Mechanochemical coupling of two substeps in a single myosin V motor
    Sotaro Uemura
    Department of Physics, School of Science and Engineering, Waseda University, 3 4 1 Okubo, Shinjuku ku, Tokyo 169 8555, Japan
    Nat Struct Mol Biol 11:877-83. 2004
  6. doi request reprint Depressed contractile performance and reduced fatigue resistance in single skinned fibers of soleus muscle after long-term disuse in rats
    Jun Udaka
    Department of Cell Physiology, The Jikei University School of Medicine, Tokyo, Japan
    J Appl Physiol (1985) 111:1080-7. 2011
  7. pmc Myosin light chain 2 modulates MgADP-induced contraction in rabbit skeletal and bovine cardiac skinned muscle
    Hideaki Fujita
    Department of Physics, School of Science and Engineering, Waseda University, 3 4 1 Okubo, Shinjuku ku, Tokyo 169 8555, Japan
    J Physiol 542:221-9. 2002
  8. ncbi request reprint Length-dependent activation and auto-oscillation in skeletal myofibrils at partial activation by Ca2+
    Yuta Shimamoto
    Department of Physics, Faculty of Science and Engineering, Waseda University, 3 4 1 Okubo, Shinjuku ku, Tokyo 169 8555, Japan
    Biochem Biophys Res Commun 366:233-8. 2008
  9. pmc Sarcomere length-dependent Ca2+ activation in skinned rabbit psoas muscle fibers: coordinated regulation of thin filament cooperative activation and passive force
    Norio Fukuda
    Department of Cell Physiology, The Jikei University School of Medicine, Tokyo, Japan
    J Physiol Sci 61:515-23. 2011
  10. pmc Regulatory mechanism of length-dependent activation in skinned porcine ventricular muscle: role of thin filament cooperative activation in the Frank-Starling relation
    Takako Terui
    Department of Cell Physiology, The Jikei University School of Medicine, Tokyo, Japan
    J Gen Physiol 136:469-82. 2010

Collaborators

Detail Information

Publications46

  1. ncbi request reprint Microscopic analysis of polymerization dynamics with individual actin filaments
    Ikuko Fujiwara
    Department of Physics, School of Science and Engineering, Waseda University, 3 4 1 Okubo, Shinjuku ku, Tokyo 169 8555, Japan
    Nat Cell Biol 4:666-73. 2002
    ....
  2. ncbi request reprint Activated protein C via PAR1 receptor regulates survival of neurons under conditions of glutamate excitotoxicity
    L R Gorbacheva
    Biology Faculty, Lomonosov Moscow State University, Moscow, 119899, Russia
    Biochemistry (Mosc) 73:717-24. 2008
    ..1 nM APC on glutamate-induced cytotoxicity in the hippocampal neurons. Thus, APC at low concentrations, activating PAR1, prevents the death of hippocampal and cortical neurons under conditions of glutamate excitotoxicity...
  3. doi request reprint Contractile system of muscle as an auto-oscillator
    Shin ichi Ishiwata
    Department of Physics, Faculty of Science and Engineering, Waseda University, 3 4 1 Okubo, Shinjuku ku, Tokyo, Japan
    Prog Biophys Mol Biol 105:187-98. 2011
    ..Finally, the physiological significance of SPOC is discussed...
  4. ncbi request reprint Regulation of muscle contraction by Ca2+ and ADP: focusing on the auto-oscillation (SPOC)
    Shin ichi Ishiwata
    Department of Physics, Faculty of Science and Engineering, Waseda University, 3 4 1 Okubo, Shinjuku ku, Tokyo 169 8555, Japan
    Adv Exp Med Biol 592:341-58. 2007
    ..This newly devised hybrid system is expected to fill the gap between the single-molecule level and the muscle system...
  5. ncbi request reprint Mechanochemical coupling of two substeps in a single myosin V motor
    Sotaro Uemura
    Department of Physics, School of Science and Engineering, Waseda University, 3 4 1 Okubo, Shinjuku ku, Tokyo 169 8555, Japan
    Nat Struct Mol Biol 11:877-83. 2004
    ..When the isomerization precedes the 12-nm substep, the 36-nm step occurs without substeps...
  6. doi request reprint Depressed contractile performance and reduced fatigue resistance in single skinned fibers of soleus muscle after long-term disuse in rats
    Jun Udaka
    Department of Cell Physiology, The Jikei University School of Medicine, Tokyo, Japan
    J Appl Physiol (1985) 111:1080-7. 2011
    ....
  7. pmc Myosin light chain 2 modulates MgADP-induced contraction in rabbit skeletal and bovine cardiac skinned muscle
    Hideaki Fujita
    Department of Physics, School of Science and Engineering, Waseda University, 3 4 1 Okubo, Shinjuku ku, Tokyo 169 8555, Japan
    J Physiol 542:221-9. 2002
    ..In other words, extraction of LC2 apparently increased the affinity of myosin for MgADP but decreased the affinity for P(i). These results demonstrate that LC2 modulates MgADP-induced activation of actomyosin interaction...
  8. ncbi request reprint Length-dependent activation and auto-oscillation in skeletal myofibrils at partial activation by Ca2+
    Yuta Shimamoto
    Department of Physics, Faculty of Science and Engineering, Waseda University, 3 4 1 Okubo, Shinjuku ku, Tokyo 169 8555, Japan
    Biochem Biophys Res Commun 366:233-8. 2008
    ..These results suggest that the force-generating properties of sarcomeres in striated muscle are determined not only by [Ca(2+)], but also by the lattice spacing as a function of SL...
  9. pmc Sarcomere length-dependent Ca2+ activation in skinned rabbit psoas muscle fibers: coordinated regulation of thin filament cooperative activation and passive force
    Norio Fukuda
    Department of Cell Physiology, The Jikei University School of Medicine, Tokyo, Japan
    J Physiol Sci 61:515-23. 2011
    ....
  10. pmc Regulatory mechanism of length-dependent activation in skinned porcine ventricular muscle: role of thin filament cooperative activation in the Frank-Starling relation
    Takako Terui
    Department of Cell Physiology, The Jikei University School of Medicine, Tokyo, Japan
    J Gen Physiol 136:469-82. 2010
    ..These findings demonstrate that the Frank-Starling relation is strongly influenced by thin filament cooperative activation...
  11. pmc Load-dependent ADP binding to myosins V and VI: implications for subunit coordination and function
    Yusuke Oguchi
    Department of Physics, Faculty of Science and Engineering, Waseda University, Tokyo 169 8555, Japan
    Proc Natl Acad Sci U S A 105:7714-9. 2008
    ..The distinct load dependence of ADP binding allows these motors to perform different cellular functions...
  12. pmc Kinesin-microtubule binding depends on both nucleotide state and loading direction
    Sotaro Uemura
    Department of Physics, School of Science and Engineering, and Advanced Research Institute for Science and Engineering, Waseda University, 3 4 1 Okubo, Shinjuku ku, Tokyo 169 8555, Japan
    Proc Natl Acad Sci U S A 99:5977-81. 2002
    ..Both these factors must play an important role in the molecular mechanism of kinesin motility...
  13. ncbi request reprint Myocardial sarcomeres spontaneously oscillate with the period of heartbeat under physiological conditions
    Daisuke Sasaki
    Integrative Bioscience and Biomedical Engineering, Graduate School of Science and Engineering, Waseda University, 3 4 1 Okubo, Shinjuku ku, Tokyo 169 8555, Japan
    Biochem Biophys Res Commun 343:1146-52. 2006
    ..On the basis of these results we propose that the intrinsic auto-oscillatory property of sarcomeres (myofibrils) significantly contributes to myocardial beating in vivo...
  14. pmc A new muscle contractile system composed of a thick filament lattice and a single actin filament
    Madoka Suzuki
    Department of Physics, School of Science and Engineering, Waseda University, Tokyo, Japan
    Biophys J 89:321-8. 2005
    ..Thus, we conclude that the A-band motility assay system described here is suitable for studying force generation on a single actin filament, and its sliding movement within a regular three-dimensional thick filament lattice...
  15. pmc Sarcomere imaging by quantum dots for the study of cardiac muscle physiology
    Fuyu Kobirumaki-Shimozawa
    Department of Cell Physiology, The Jikei University School of Medicine, Minato ku, Tokyo, Japan
    J Biomed Biotechnol 2012:313814. 2012
    ..We propose that QDs are the ideal tool for the study of sarcomere dynamics during excitation-contraction coupling in healthy and diseased cardiac muscle...
  16. pmc Mechanical impulses can control metaphase progression in a mammalian cell
    Takeshi Itabashi
    Department of Physics, Faculty of Science and Engineering, Waseda University, 3 4 1 Okubo, Shinjuku, Tokyo 169 8555, Japan
    Proc Natl Acad Sci U S A 109:7320-5. 2012
    ..Thus, the external mechanical force acts as a crucial regulator for metaphase progression, modulating the internal force balance and thereby triggering specific mechanochemical cellular reactions...
  17. doi request reprint The bidirectional depolymerizer MCAK generates force by disassembling both microtubule ends
    Yusuke Oguchi
    Department of Physics, Faculty of Science and Engineering, Waseda University, 3 4 1 Okubo, Shinjuku ku, Tokyo 169 8555, Japan
    Nat Cell Biol 13:846-52. 2011
    ..These results provide a simple model for the generation of driving force and the regulation of chromosome segregation by the activity of MCAK at both kinetochores and spindle poles through a 'side-sliding, end-catching' mechanism...
  18. pmc Inter-sarcomere coordination in muscle revealed through individual sarcomere response to quick stretch
    Yuta Shimamoto
    Department of Physics, Faculty of Science and Engineering, Waseda University, 3 4 1 Okubo, Shinjuku ku, Tokyo 169 8555, Japan
    Proc Natl Acad Sci U S A 106:11954-9. 2009
    ..These results strongly suggest that the contractile system of muscle possesses the mechanism of structure-based inter-sarcomere coordination...
  19. ncbi request reprint Visualization and force measurement of branching by Arp2/3 complex and N-WASP in actin filament
    Ikuko Fujiwara
    Department of Physics, School of Science and Engineering, Waseda University, 3 4 1 Okubo, Shinjuku, Tokyo 169 8555, Japan
    Biochem Biophys Res Commun 293:1550-5. 2002
    ..We found, by single molecule measurements using optical tweezers, that the Arp2/3 complex attaches to the side of actin filaments and the N-WASP appears to detach from the actin-Arp2/3 complex at 6-7 pN...
  20. pmc Insights into the mechanisms of myosin and kinesin molecular motors from the single-molecule unbinding force measurements
    Sergey V Mikhailenko
    Department of Physics, Faculty of Science and Engineering, Waseda University, Tokyo 169 8555, Japan
    J R Soc Interface 7:S295-306. 2010
    ..We review the application of this technique to various linear molecular motors, both processive and non-processive, giving special attention to the importance of the experimental geometry...
  21. ncbi request reprint Molecular synchronization in actomyosin motors --from single molecule to muscle fiber via nanomuscle
    Shin ichi Ishiwata
    Department of Physics, School of Science and Engineering, Advanced Research Institute for Science and Engineering, Waseda University, 3 4 1 Okubo, Shinjuku ku, Tokyo 169 8555, Japan
    Adv Exp Med Biol 565:25-35; discussion 35-6, 359-69. 2005
  22. ncbi request reprint Auto-oscillations of skinned myocardium correlating with heartbeat
    Daisuke Sasaki
    Integrative Bioscience and Biomedical Engineering, Graduate School of Science and Engineering, Waseda University, 3 4 1 Okubo, Shinjuku ku, Tokyo, 169 8555, Japan
    J Muscle Res Cell Motil 26:93-101. 2005
    ....
  23. doi request reprint Microscopic heat pulses induce contraction of cardiomyocytes without calcium transients
    Kotaro Oyama
    Department of Physics, School of Advanced Science and Engineering, Waseda University, Tokyo, Japan
    Biochem Biophys Res Commun 417:607-12. 2012
    ..This heat pulse-induced Ca(2+)-decoupled contraction technique has the potential to stimulate heart and skeletal muscles in a manner different from the conventional electrical stimulations...
  24. pmc The role of tropomyosin domains in cooperative activation of the actin-myosin interaction
    Yusuke Oguchi
    Department of Physics, Waseda University, 3 4 1 Okubo, Shinjuku ku, Tokyo 169 8555, Japan
    J Mol Biol 414:667-80. 2011
    ..These results are consistent with the mechanism that the Tm-actin interaction cooperatively affects actin to result in generation of greater force and velocity...
  25. doi request reprint A nanoparticle-based ratiometric and self-calibrated fluorescent thermometer for single living cells
    Yoshiaki Takei
    Department of Life Science and Medical Bioscience, Graduate School of Advanced Science and Engineering, Waseda University, 2 2 TWIns, Wakamatsu cho, Shinjuku ku, Tokyo, 162 8480 Japan
    ACS Nano 8:198-206. 2014
    ..The average temperature elevation within a cell is positively correlated to the increase in Ca(2+), suggesting that the activity and/or number of heat sources are dependent on the Ca(2+) concentration. ..
  26. pmc Disuse-induced preferential loss of the giant protein titin depresses muscle performance via abnormal sarcomeric organization
    Jun Udaka
    Department of Cell Physiology, The Jikei University, Tokyo, Japan
    J Gen Physiol 131:33-41. 2008
    ..These results provide a novel view that disuse-induced preferential titin loss results in altered muscle function via abnormal sarcomeric organization...
  27. doi request reprint Real-time measurement of the length of a single sarcomere in rat ventricular myocytes: a novel analysis with quantum dots
    Takahiro Serizawa
    Department of Cell Physiology, The Jikei University School of Medicine, Tokyo, Japan
    Am J Physiol Cell Physiol 301:C1116-27. 2011
    ..Therefore, QDs are a useful tool to quantitatively analyze the movement of single sarcomeres in cardiomyocytes, under various experimental settings...
  28. ncbi request reprint Loading direction regulates the affinity of ADP for kinesin
    Sotaro Uemura
    Department of Physics, School of Science and Engineering, Waseda University, 3 4 1 Okubo, Shinjuku ku, Tokyo 169 8555, Japan
    Nat Struct Biol 10:308-11. 2003
    ..This coupling between loading direction and enzymatic activity is in accord with the idea that the internal load plays a key role in the unidirectional and cooperative movement of processive motors...
  29. pmc D-loop of actin differently regulates the motor function of myosins II and V
    Hiroaki Kubota
    Department of Physics, Faculty of Science and Engineering, Waseda University, 3 4 1 Okubo, Shinjuku ku, Tokyo, Japan
    J Biol Chem 284:35251-8. 2009
    ..These results show that the D-loop strongly modulates the force generation by myosin II and the processivity of myosin V, presumably affecting actin-myosin interaction in the actomyosin-ADP.P(i) state of both myosins...
  30. doi request reprint Micromechanics of the vertebrate meiotic spindle examined by stretching along the pole-to-pole axis
    Jun Takagi
    Department of Physics, Faculty of Science and Engineering, Waseda University, Tokyo, Japan
    Biophys J 106:735-40. 2014
    ..In addition, both the volume of the spindle and the tubulin density are conserved under the stretching force. These results provide insight into how the spindle size is maintained at metaphase. ..
  31. doi request reprint Using micromanipulation to analyze control of vertebrate meiotic spindle size
    Jun Takagi
    Department of Physics, Faculty of Science and Engineering, Waseda University, 3 4 1 Okubo, Shinjuku ku, Tokyo 169 8555, Japan
    Cell Rep 5:44-50. 2013
    ..The equation we obtained quantitatively describes how the spindle size correlates with the amount of MTs while maintaining the shape and the MT density. ..
  32. pmc Mechanical distortion of single actin filaments induced by external force: detection by fluorescence imaging
    Togo Shimozawa
    Department of Physics, Faculty of Science and Engineering, Waseda University, Tokyo, Japan
    Biophys J 96:1036-44. 2009
    ..We thus conclude that the fluorescent dye incorporated into an appropriate site of actin can report the mechanical distortion of the binding site, which is a necessary condition for the bio-nano strain gauge...
  33. doi request reprint Improvement of the yields of recombinant actin and myosin V-HMM in the insect cell/baculovirus system by the addition of nutrients to the high-density cell culture
    Takashi Ohki
    Department of Physics, Faculty of Science and Engineering, Waseda University, 3 4 1 Okubo, Tokyo 169 8555, Japan
    J Muscle Res Cell Motil 33:351-8. 2012
    ..The functionality of the recombinant actin and myosin V-HMM was confirmed by measuring the rate of actin polymerization, actin-activated ATPase, and the gliding velocity of actin filaments in an in vitro motility assay...
  34. doi request reprint Modulation of the mechano-chemical properties of myosin V by drebrin-E
    Hiroaki Kubota
    Department of Physics, Faculty of Science and Engineering, Waseda University, 3 4 1 Okubo, Shinjuku ku, Tokyo 169 8555, Japan
    Biochem Biophys Res Commun 400:643-8. 2010
    ..Our study indicates that, in addition to the regulation of binding affinity of myosin V, drebrin-E also modulates the chemo-mechanical coupling in the motile myosin V molecules, presumably affecting the movement of the growth cone...
  35. doi request reprint Regulatory mechanism of smooth muscle contraction studied with gelsolin-treated strips of taenia caeci in guinea pig
    Ying Ming Liou
    Dept of Physics, Waseda University, Tokyo 169 8555, Japan
    Am J Physiol Cell Physiol 296:C1024-33. 2009
    ..We thus conclude that Tm and CaD are necessary for the full regulation of smooth muscle contraction in addition to the other regulatory systems, including the myosin-linked one...
  36. doi request reprint A theory on auto-oscillation and contraction in striated muscle
    Katsuhiko Sato
    Department of Physics, Faculty of Science, Tohoku University, Aoba, Sendai, Japan
    Prog Biophys Mol Biol 105:199-207. 2011
    ..Thus, the present theory comprehensively explains the characteristics of auto-oscillation and contraction in the contractile system of striated muscle...
  37. ncbi request reprint A novel method of thermal activation and temperature measurement in the microscopic region around single living cells
    Vadim Zeeb
    Department of Physics, School of Science and Engineering, Waseda University, 3 4 1 Okubo, Shinjuku ku, Tokyo 169 8555, Japan
    J Neurosci Methods 139:69-77. 2004
    ..The microm size of the tip of this pipette was able to measure the local temperature with 0.1 degrees C precision and microm spatial resolution. This new approach is compatible with standard electrophysiological and imaging techniques...
  38. doi request reprint Walking nanothermometers: spatiotemporal temperature measurement of transported acidic organelles in single living cells
    Kotaro Oyama
    Department of Physics, School of Advanced Science and Engineering, Waseda University, Tokyo, Japan
    Lab Chip 12:1591-3. 2012
    ..e., endosome/lysosome, and then transported along microtubules in a temperature-dependent manner, working as "walking nanothermometers"...
  39. ncbi request reprint Mass spectrometric screening of ligands with lower off-rate from a clicked-based pooled library
    Satoshi Arai
    Consolidated Research Institute for Advanced Science and Medical Care, Waseda University, 513 Wasedatsurumaki cho, Tokyo 162 0041, Japan
    ACS Comb Sci 14:451-5. 2012
    ..e., off-rates in solution. Finally, we prepared a fluorescent probe utilizing the ligand with lowest off-rate and succeeded in performing single molecule observations of hexahistidine-tagged myosin V walking on actin filaments...
  40. doi request reprint Purification of cytoplasmic actin by affinity chromatography using the C-terminal half of gelsolin
    Takashi Ohki
    Department of Physics, Waseda University, Okubo, Shinjuku ku, Tokyo, Japan
    Biochem Biophys Res Commun 383:146-50. 2009
    ....
  41. pmc Titin and troponin: central players in the frank-starling mechanism of the heart
    Norio Fukuda
    Department of Cell Physiology, The Jikei University School of Medicine, Tokyo, Japan
    Curr Cardiol Rev 5:119-24. 2009
    ..In this review, we discuss how myocardial length-dependent activation is coordinately regulated by sarcomere proteins...
  42. pmc Chromosome position at the spindle equator is regulated by chromokinesin and a bipolar microtubule array
    Jun Takagi
    1 Department of Physics, Faculty of Science and Engineering, Waseda University, 3 4 1 Okubo, Shinjuku, Tokyo 169 8555, Japan 2
    Sci Rep 3:2808. 2013
    ....
  43. pmc Probing the mechanical architecture of the vertebrate meiotic spindle
    Takeshi Itabashi
    Department of Physics, Faculty of Science and Engineering, Waseda University, 3 4 1 Okubo, Shinjuku, Tokyo 169 8555, Japan
    Nat Methods 6:167-72. 2009
    ..The technique we describe here may also be useful for examining the micromechanics of other cellular organelles...
  44. pmc Robust processivity of myosin V under off-axis loads
    Yusuke Oguchi
    Department of Physics, Faculty of Science and Engineering, Waseda University, Tokyo, Japan
    Nat Chem Biol 6:300-5. 2010
    ....
  45. pmc Equilibrium and transition between single- and double-headed binding of kinesin as revealed by single-molecule mechanics
    Kenji Kawaguchi
    Department of Physics, School of Science and Engineering, Waseda University, 3 4 1 Okubo, Shinjuku ku, Tokyo 169 8555, Japan
    Biophys J 84:1103-13. 2003
    ....
  46. pmc Quasiperiodic distribution of rigor cross-bridges along a reconstituted thin filament in a skeletal myofibril
    Madoka Suzuki
    Waseda Bioscience Research Institute in Singapore, Singapore
    Biophys J 101:2740-8. 2011
    ..Additionally, the unbinding force (i.e., the binding affinity) of CBs for the reconstituted thin filaments was found to be larger and smaller relative to that for actin filaments with and without Ca(2+), respectively...