Research Topics
| Shin ichi IshiwataSummaryAffiliation: Waseda University Country: Japan Publications
| Collaborators
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Detail Information
Publications
Microscopic analysis of polymerization dynamics with individual actin filamentsIkuko Fujiwara
Department of Physics, School of Science and Engineering, Waseda University, 3 4 1 Okubo, Shinjuku ku, Tokyo 169 8555, Japan
Nat Cell Biol 4:666-73. 2002....- Regulation of muscle contraction by Ca2+ and ADP: focusing on the auto-oscillation (SPOC)Shin ichi Ishiwata
Department of Physics, Faculty of Science and Engineering, Waseda University, 3 4 1 Okubo, Shinjuku ku, Tokyo 169 8555, Japan
Adv Exp Med Biol 592:341-58. 2007..This newly devised hybrid system is expected to fill the gap between the single-molecule level and the muscle system... 
Contractile system of muscle as an auto-oscillatorShin ichi Ishiwata
Department of Physics, Faculty of Science and Engineering, Waseda University, 3 4 1 Okubo, Shinjuku ku, Tokyo, Japan
Prog Biophys Mol Biol 105:187-98. 2011..Finally, the physiological significance of SPOC is discussed...- Depressed contractile performance and reduced fatigue resistance in single skinned fibers of soleus muscle after long-term disuse in ratsJun Udaka
Department of Cell Physiology, The Jikei University School of Medicine, Tokyo, Japan
J Appl Physiol 111:1080-7. 2011.... 
Sarcomere length-dependent Ca2+ activation in skinned rabbit psoas muscle fibers: coordinated regulation of thin filament cooperative activation and passive forceNorio Fukuda
Department of Cell Physiology, The Jikei University School of Medicine, Tokyo, Japan
J Physiol Sci 61:515-23. 2011....- Sarcomere imaging by quantum dots for the study of cardiac muscle physiologyFuyu Kobirumaki-Shimozawa
Department of Cell Physiology, The Jikei University School of Medicine, Minato ku, Tokyo, Japan
J Biomed Biotechnol 2012:313814. 2012..We propose that QDs are the ideal tool for the study of sarcomere dynamics during excitation-contraction coupling in healthy and diseased cardiac muscle... - Mechanical impulses can control metaphase progression in a mammalian cellTakeshi Itabashi
Department of Physics, Faculty of Science and Engineering, Waseda University, 3 4 1 Okubo, Shinjuku, Tokyo 169 8555, Japan
Proc Natl Acad Sci U S A 109:7320-5. 2012..Thus, the external mechanical force acts as a crucial regulator for metaphase progression, modulating the internal force balance and thereby triggering specific mechanochemical cellular reactions... - Inter-sarcomere coordination in muscle revealed through individual sarcomere response to quick stretchYuta Shimamoto
Department of Physics, Faculty of Science and Engineering, Waseda University, 3 4 1 Okubo, Shinjuku ku, Tokyo 169 8555, Japan
Proc Natl Acad Sci U S A 106:11954-9. 2009..quot; These results strongly suggest that the contractile system of muscle possesses the mechanism of structure-based inter-sarcomere coordination... - The bidirectional depolymerizer MCAK generates force by disassembling both microtubule endsYusuke Oguchi
Department of Physics, Faculty of Science and Engineering, Waseda University, 3 4 1 Okubo, Shinjuku ku, Tokyo 169 8555, Japan
Nat Cell Biol 13:846-52. 2011..These results provide a simple model for the generation of driving force and the regulation of chromosome segregation by the activity of MCAK at both kinetochores and spindle poles through a 'side-sliding, end-catching' mechanism... - Load-dependent ADP binding to myosins V and VI: implications for subunit coordination and functionYusuke Oguchi
Department of Physics, Faculty of Science and Engineering, Waseda University, Tokyo 169 8555, Japan
Proc Natl Acad Sci U S A 105:7714-9. 2008..The distinct load dependence of ADP binding allows these motors to perform different cellular functions... - Microscopic heat pulses induce contraction of cardiomyocytes without calcium transientsKotaro Oyama
Department of Physics, School of Advanced Science and Engineering, Waseda University, Tokyo, Japan
Biochem Biophys Res Commun 417:607-12. 2012..This heat pulse-induced Ca(2+)-decoupled contraction technique has the potential to stimulate heart and skeletal muscles in a manner different from the conventional electrical stimulations... - The role of tropomyosin domains in cooperative activation of the actin-myosin interactionYusuke Oguchi
Department of Physics, Waseda University, 3 4 1 Okubo, Shinjuku ku, Tokyo 169 8555, Japan
J Mol Biol 414:667-80. 2011..These results are consistent with the mechanism that the Tm-actin interaction cooperatively affects actin to result in generation of greater force and velocity... - Regulatory mechanism of length-dependent activation in skinned porcine ventricular muscle: role of thin filament cooperative activation in the Frank-Starling relationTakako Terui
Department of Cell Physiology, The Jikei University School of Medicine, Tokyo, Japan
J Gen Physiol 136:469-82. 2010..These findings demonstrate that the Frank-Starling relation is strongly influenced by thin filament cooperative activation... - Length-dependent activation and auto-oscillation in skeletal myofibrils at partial activation by Ca2+Yuta Shimamoto
Department of Physics, Faculty of Science and Engineering, Waseda University, 3 4 1 Okubo, Shinjuku ku, Tokyo 169 8555, Japan
Biochem Biophys Res Commun 366:233-8. 2008..These results suggest that the force-generating properties of sarcomeres in striated muscle are determined not only by [Ca(2+)], but also by the lattice spacing as a function of SL... - Real-time measurement of the length of a single sarcomere in rat ventricular myocytes: a novel analysis with quantum dotsTakahiro Serizawa
Department of Cell Physiology, The Jikei University School of Medicine, Tokyo, Japan
Am J Physiol Cell Physiol 301:C1116-27. 2011..Therefore, QDs are a useful tool to quantitatively analyze the movement of single sarcomeres in cardiomyocytes, under various experimental settings... - Insights into the mechanisms of myosin and kinesin molecular motors from the single-molecule unbinding force measurementsSergey V Mikhailenko
Department of Physics, Faculty of Science and Engineering, Waseda University, Tokyo 169 8555, Japan
J R Soc Interface 7:S295-306. 2010..We review the application of this technique to various linear molecular motors, both processive and non-processive, giving special attention to the importance of the experimental geometry... - D-loop of actin differently regulates the motor function of myosins II and VHiroaki Kubota
Department of Physics, Faculty of Science and Engineering, Waseda University, 3 4 1 Okubo, Shinjuku ku, Tokyo, Japan
J Biol Chem 284:35251-8. 2009..These results show that the D-loop strongly modulates the force generation by myosin II and the processivity of myosin V, presumably affecting actin-myosin interaction in the actomyosin-ADP.P(i) state of both myosins... - Improvement of the yields of recombinant actin and myosin V-HMM in the insect cell/baculovirus system by the addition of nutrients to the high-density cell cultureTakashi Ohki
Department of Physics, Faculty of Science and Engineering, Waseda University, 3 4 1 Okubo, Tokyo 169 8555, Japan
J Muscle Res Cell Motil 33:351-8. 2012..The functionality of the recombinant actin and myosin V-HMM was confirmed by measuring the rate of actin polymerization, actin-activated ATPase, and the gliding velocity of actin filaments in an in vitro motility assay... - Regulatory mechanism of smooth muscle contraction studied with gelsolin-treated strips of taenia caeci in guinea pigYing Ming Liou
Dept of Physics, Waseda University, Tokyo 169 8555, Japan
Am J Physiol Cell Physiol 296:C1024-33. 2009..We thus conclude that Tm and CaD are necessary for the full regulation of smooth muscle contraction in addition to the other regulatory systems, including the myosin-linked one... - Modulation of the mechano-chemical properties of myosin V by drebrin-EHiroaki Kubota
Department of Physics, Faculty of Science and Engineering, Waseda University, 3 4 1 Okubo, Shinjuku ku, Tokyo 169 8555, Japan
Biochem Biophys Res Commun 400:643-8. 2010..Our study indicates that, in addition to the regulation of binding affinity of myosin V, drebrin-E also modulates the chemo-mechanical coupling in the motile myosin V molecules, presumably affecting the movement of the growth cone... - Walking nanothermometers: spatiotemporal temperature measurement of transported acidic organelles in single living cellsKotaro Oyama
Department of Physics, School of Advanced Science and Engineering, Waseda University, Tokyo, Japan
Lab Chip 12:1591-3. 2012..e., endosome/lysosome, and then transported along microtubules in a temperature-dependent manner, working as "walking nanothermometers"... - Purification of cytoplasmic actin by affinity chromatography using the C-terminal half of gelsolinTakashi Ohki
Department of Physics, Waseda University, Okubo, Shinjuku ku, Tokyo, Japan
Biochem Biophys Res Commun 383:146-50. 2009.... - Mass spectrometric screening of ligands with lower off-rate from a clicked-based pooled librarySatoshi Arai
Consolidated Research Institute for Advanced Science and Medical Care, Waseda University, 513 Wasedatsurumaki cho, Tokyo 162 0041, Japan
ACS Comb Sci 14:451-5. 2012..e., off-rates in solution. Finally, we prepared a fluorescent probe utilizing the ligand with lowest off-rate and succeeded in performing single molecule observations of hexahistidine-tagged myosin V walking on actin filaments... - Disuse-induced preferential loss of the giant protein titin depresses muscle performance via abnormal sarcomeric organizationJun Udaka
Department of Cell Physiology, The Jikei University, Tokyo, Japan
J Gen Physiol 131:33-41. 2008..These results provide a novel view that disuse-induced preferential titin loss results in altered muscle function via abnormal sarcomeric organization... - Titin and troponin: central players in the frank-starling mechanism of the heartNorio Fukuda
Department of Cell Physiology, The Jikei University School of Medicine, Tokyo, Japan
Curr Cardiol Rev 5:119-24. 2009..In this review, we discuss how myocardial length-dependent activation is coordinately regulated by sarcomere proteins... - Mechanical distortion of single actin filaments induced by external force: detection by fluorescence imagingTogo Shimozawa
Department of Physics, Faculty of Science and Engineering, Waseda University, Tokyo, Japan
Biophys J 96:1036-44. 2009..We thus conclude that the fluorescent dye incorporated into an appropriate site of actin can report the mechanical distortion of the binding site, which is a necessary condition for the bio-nano strain gauge... - A theory on auto-oscillation and contraction in striated muscleKatsuhiko Sato
Department of Physics, Faculty of Science, Tohoku University, Aoba, Sendai, Japan
Prog Biophys Mol Biol 105:199-207. 2011..Thus, the present theory comprehensively explains the characteristics of auto-oscillation and contraction in the contractile system of striated muscle... - Probing the mechanical architecture of the vertebrate meiotic spindleTakeshi Itabashi
Department of Physics, Faculty of Science and Engineering, Waseda University, 3 4 1 Okubo, Shinjuku, Tokyo 169 8555, Japan
Nat Methods 6:167-72. 2009..The technique we describe here may also be useful for examining the micromechanics of other cellular organelles... - Robust processivity of myosin V under off-axis loadsYusuke Oguchi
Department of Physics, Faculty of Science and Engineering, Waseda University, Tokyo, Japan
Nat Chem Biol 6:300-5. 2010.... - Quasiperiodic distribution of rigor cross-bridges along a reconstituted thin filament in a skeletal myofibrilMadoka Suzuki
Waseda Bioscience Research Institute in Singapore, Singapore
Biophys J 101:2740-8. 2011..Additionally, the unbinding force (i.e., the binding affinity) of CBs for the reconstituted thin filaments was found to be larger and smaller relative to that for actin filaments with and without Ca(2+), respectively...